A machine-free nucleic acid extraction tube and extraction method

Abstract

The invention relates to the technical field of nucleic acid extraction tubes, and discloses a machine-free nucleic acid extraction tube and an extraction method, comprising a main body, a piston rod is slidably arranged inside the main body, an operating tube is arranged at the bottom end of the main body, a mounting block is arranged outside the lower part of the main body, a plurality of groups of reagent tubes are arranged around the outside of the main body, a connecting tube is connected between the mounting block and the reagent tube, a movable block penetrates the outside of the connecting tube, and the movable block is slidably arranged along the axial direction of the connecting tube; the connecting tube comprises an outer sleeve, an inner hose is penetrated inside the outer sleeve, an extrusion rod is installed on the movable block, and two groups of extrusion rods are arranged corresponding to the two sides of the inner hose, and the two groups of extrusion rods are close to each other for extruding the inner hose and squeezing the reagent inside the inner hose to the mounting block; the invention pushes the liquid reagent remaining in the inner hose into the main body by arranging a movable movable block and an extrusion rod, so as to ensure that the dosage of the liquid reagent introduced into the main body is sufficient.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid extraction tubes, and more specifically, to a nucleic acid extraction tube and an extraction method that do not require a machine. Background Art

[0002] A nucleic acid extraction tube is a tool used to separate DNA or RNA from biological samples. It usually contains chemical reagents that can lyse sample cells and protect nucleic acids from degradation. Nucleic acid extraction can be performed without the need for specialized machinery and equipment. Some simple manual methods can be used, which mainly rely on the action of chemical reagents to complete the nucleic acid extraction process.

[0003] During the manual nucleic acid extraction process, several different types of liquid reagents are used to help lyse cells, purify nucleic acids and remove impurities. Liquid reagents include lysis solutions, detergents and buffers. In order to avoid cross-infection between liquid reagents, the existing technology uses multiple reagent tubes on the outside of the main tube. Different reagent tubes store different liquid reagents, and then various liquid reagents are squeezed into the main tube in sequence.

[0004] In order to reduce the loss of liquid reagents caused by adsorption on the inner wall of the tube during transportation, the prior art sets the connection position between the reagent tube and the main tube at the lower part of the main tube. When the required types of liquid reagents are large, the number of reagent tubes set increases. Since the connection position between the reagent tube and the main tube has been determined, and the number of reagent tubes is limited by the outer area of ​​the main tube, when the diameter of the main tube remains unchanged, the need to connect multiple reagent tubes on the outside of the main tube can be met by increasing the length of the connecting tube between the reagent tube and the main tube.

[0005] When a high-concentration liquid reagent is used for operation, part of the high-concentration liquid reagent will adhere to the inner wall of the connecting tube, resulting in insufficient dosage of the liquid reagent introduced into the main tube. Summary of the invention

[0006] The present invention provides a machine-free nucleic acid extraction tube and extraction method, and aims to solve the problem that when the existing machine-free nucleic acid extraction tube is operated with a large amount of liquid reagents with high viscosity, the dosage of the liquid reagent introduced into the main tube may be insufficient or the liquid reagent after the operation inside the main tube may be difficult to squeeze out.

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a nucleic acid extraction tube that does not require a machine, comprising a main body, a piston rod is slidably provided inside the main body, an operating tube is provided at the bottom end of the main body, a mounting block is provided on the outer side of the lower part of the main body, a plurality of groups of reagent tubes are arranged around the outer side of the main body, a connecting tube is connected between the mounting block and the reagent tube, a movable block passes through the outer side of the connecting tube, and the movable block is slidably arranged along the axial direction of the connecting tube; the connecting tube comprises an outer sleeve, an inner hose passes through the inner side of the outer sleeve, an extrusion rod is installed on the movable block, and two groups of extrusion rods are arranged corresponding to the two sides of the inner hose, and the two groups of extrusion rods are close to each other for extruding the inner hose and squeezing the reagent inside the inner hose to the mounting block.

[0008] In a preferred embodiment, a guide groove is formed on the inner wall of the outer sleeve, and guide blocks are fixedly provided on both sides of the movable block, and the guide blocks are slidably arranged along the inside of the corresponding guide groove.

[0009] In a preferred embodiment, a reset mechanism is installed on the inner wall of the outer sleeve. When the two groups of extrusion rods squeeze the inner hose, a bulge is generated so that the inner hose moves away from the extrusion point. The reset mechanism is arranged corresponding to the bulge and is used to reset the bulge.

[0010] In a preferred embodiment, the reset mechanism includes a hollow tube with an airbag installed on it. The movable block and the airbag are connected via the hollow tube. An inflation component is installed inside the movable block. When the extrusion rod moves away from the inner hose, the airbag is inflated through the inflation component.

[0011] In a preferred embodiment, the inflation component includes a push plate, a piston rod 2 is fixedly provided on one side of the push plate corresponding to the movable block, a piston block 1 is fixedly provided at one end of the piston rod 2, an air cavity is opened inside the movable block, the piston block 1 is slidably arranged along the inside of the corresponding air cavity, the push plate is fixedly connected to the extrusion rod, and when the piston rod 2 moves in a direction away from the inner hose, the gas inside the air cavity is filled into the interior of the airbag.

[0012] In a preferred embodiment, an air channel 1 is opened inside the movable block, and the air channel 1 is located outside the air cavity, and an end of the air cavity away from the inner hose is connected to one end of the air channel 1.

[0013] In a preferred embodiment, the diameter of the main pipe is enlarged to twice the original diameter, so that the distance between two adjacent groups of mounting blocks along the circumferential direction is increased to twice.

[0014] In a preferred embodiment, a piston block 2 is fixedly provided at one end of the piston rod 1, and the piston block 2 is slidably arranged inside the main pipe body, an air duct runs through the interior of the piston block 2, the input end of the air duct is located at the top end of the piston rod 1, and the output end of the air duct is located at the bottom end of the piston block 2.

[0015] In a preferred embodiment, a connecting block is fixedly provided at the bottom end of the air guide tube, a rubber plate is fixedly provided at the bottom of the second piston block, and the connecting block is fixedly connected to the middle part of the rubber plate, a groove is provided at the bottom of the second piston block, and an air hole is provided in the middle part of the rubber plate, and the middle part of the rubber plate is pulled upward by the air guide tube to bulge into the interior of the groove.

[0016] The present invention also provides a method for extracting nucleic acid without a machine, comprising the following steps:

[0017] S1: Prepare biological samples and send them into the host body;

[0018] S2: squeeze one of the liquid reagents into the main tube through one of the reagent tubes;

[0019] S3: Push the liquid reagent adhered to the connecting tube into the main tube by squeezing the rod;

[0020] S4: squeeze another liquid reagent into the main body through another set of reagent tubes, and repeat step S3;

[0021] S5: Repeat steps S2-S4 to perform nucleic acid extraction on the biological sample.

[0022] The beneficial effects of the present invention are:

[0023] The present invention pushes the residual liquid reagent in the inner hose into the inner main body by arranging a movable active block and an extrusion rod, thereby reducing the high-concentration liquid reagent adhering to the inner wall of the inner hose and ensuring that the dosage of the liquid reagent introduced into the main body is sufficient.

[0024] The present invention can separate the inner walls of the inner hose that are stuck together by arranging a reset mechanism. When the squeezing rod removes the squeezing force, the inner walls of the inner hose corresponding to the two sides are stuck together by a high-concentration liquid reagent, and the airbag is inflated and expands toward the inside of the outer sleeve, squeezing the protruding part of the inner hose back to its original position. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the main body structure of the present invention.

[0026] Figure 2 It is a schematic diagram of the side cross-sectional structure of the connecting pipe of the present invention.

[0027] Figure 3 It is a top view schematic diagram of the connecting pipe structure of the present invention.

[0028] Figure 4 It is a schematic top view of the reset mechanism structure of the present invention.

[0029] Figure 5 It is a schematic top view of the cross-sectional structure of the movable block of the present invention.

[0030] Figure 6 This is a schematic diagram of the extrusion rod of the present invention performing extrusion work.

[0031] Figure 7 This is a schematic diagram of the retracting operation of the extrusion rod of the present invention.

[0032] Figure 8 It is a schematic diagram of the structure after the diameter of the main pipe of the present invention is enlarged.

[0033] Fig. 9 It is a schematic diagram of the side cross-section structure of the main body of the present invention.

[0034] Fig.10 It is a schematic diagram of the two-side cross-sectional structure of the piston block of the present invention.

[0035] Fig.11 It is a schematic diagram of the extraction method of the present invention.

[0036] The accompanying drawings are marked as follows: 1. main body; 11. piston rod 1; 12. operating tube; 2. mounting block; 3. reagent tube; 4. connecting tube; 41. outer sleeve; 42. inner hose; 43. guide groove; 5. movable block; 51. guide block; 52. air cavity; 53. airway 1; 6. extrusion rod; 61. push plate; 62. piston rod 2; 63. piston block 1; 7. reset mechanism; 71. hollow tube; 72. air bag; 8. piston block 2; 81. air guide tube; 82. connecting block; 83. rubber plate; 831. air hole; 84. groove. DETAILED DESCRIPTION

[0037] The present application is further described in detail below in conjunction with the accompanying drawings. It is necessary to point out here that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technical personnel in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0038] Refer to the instruction manual Figures 1 to 7 A machine-free nucleic acid extraction tube comprises a main body 1, a piston rod 11 is slidably provided inside the main body 1, an operating tube 12 is provided at the bottom end of the main body 1, a mounting block 2 is provided on the outer side of the lower part of the main body 1, and a plurality of groups of reagent tubes 3 are arranged around the outer side of the main body 1, a connecting tube 4 is connected between the mounting block 2 and the reagent tubes 3, a movable block 5 is passed through the outer side of the connecting tube 4, and the movable block 5 is slidably arranged along the axial direction of the connecting tube 4; the connecting tube 4 comprises an outer sleeve 41, an inner hose 42 is passed through the inner side of the outer sleeve 41, an extrusion rod 6 is installed on the upper side of the movable block 5, and two groups of extrusion rods 6 are arranged corresponding to the two sides of the inner hose 42, and the two groups of extrusion rods 6 are close to each other for extruding the inner hose 42 and squeezing the reagent inside the inner hose 42 to the mounting block 2.

[0039] It should be noted that the operation tube 12 is a nucleic acid adsorption operation tube of the prior art, which is used to collect captured nucleic acids. A discharge port is provided at the bottom end of the operation tube 12 for discharging waste liquid and captured nucleic acids. The specific steps refer to the nucleic acid extraction device of the prior art. A one-way valve is provided at the connection between the reagent tube 3 and the connecting tube 4 or at the connection between the mounting block 2 and the connecting tube 4. The liquid reagent flows from the reagent tube 3 to the connecting tube 4, the mounting block 2 and the main body 1 in sequence. The reagent tube 3 is a soft rubber tube. The extrusion rod 6 passes through the middle of the movable block 5, and the extrusion rod 6 is arranged to slide horizontally. The outer sleeve 41 is a hard tube for supporting the reagent tube 3 and the inner hose 42.

[0040] In this embodiment, the implementation scenario is specifically as follows: the reagent tube 3 is squeezed by fingers to squeeze the liquid reagent inside the reagent tube 3 into the connecting tube 4, and then the liquid reagent is allowed to enter the main body 1 through the mounting block 2. When part of the high-concentration liquid reagent sticks to the inner wall of the connecting tube 4, the movable block 5 is moved to the upper end close to the reagent tube 3, and the two sets of squeezing rods 6 are pressed to make them close to each other, so that the inner hose 42 can be squeezed from both sides, and then the movable block 5 is moved downward to make the squeezing rod 6 push the liquid reagent remaining inside the inner hose 42 to flow downward until the liquid reagent reaches the mounting block 2 and enters the main body 1. By setting the movable movable block 5 and the squeezing rod 6 as a whole, the liquid reagent remaining inside the inner hose 42 is pushed into the main body 1, thereby reducing the high-concentration liquid reagent sticking to the inner wall of the inner hose 42 and ensuring that the dosage of the liquid reagent entering the main body 1 is sufficient.

[0041] Furthermore, a guide groove 43 is formed on the inner wall of the outer sleeve 41 , and guide blocks 51 are fixedly provided on both sides of the movable block 5 , and the guide blocks 51 are slidably arranged along the inside of the corresponding guide groove 43 .

[0042] It should be noted that the guide block 51 and the guide groove 43 are both arranged in an arc shape, and when the movable block 5 slides along the axial direction of the outer sleeve 41 , the guide block 51 and the guide groove 43 play a guiding role.

[0043] Furthermore, a reset mechanism 7 is installed on the inner wall of the outer sleeve 41. When the two groups of extrusion rods 6 squeeze the inner hose 42, a bulge is generated so that the inner hose 42 is away from the extrusion position. The reset mechanism 7 is set corresponding to the bulge and is used to reset the bulge.

[0044] It should be noted that, when the squeezing rod 6 removes the squeezing force, the inner walls on both sides of the inner hose 42 corresponding to each other are stuck together by the high-concentration liquid reagent, and the inner walls on both sides of the inner hose 42 that are stuck together can be separated by the reset mechanism 7 .

[0045] Furthermore, the reset mechanism 7 includes a hollow tube 71, on which an airbag 72 is installed. The movable block 5 and the airbag 72 are connected via the hollow tube 71. An inflation component is installed inside the movable block 5. When the extrusion rod 6 moves away from the inner hose 42, the airbag 72 is inflated through the inflation component.

[0046] It should be noted that the hollow tube 71 is hollow, and the airbag 72 is arranged corresponding to the protruding portion. After the airbag 72 is inflated, it expands toward the inside of the outer sleeve 41.

[0047] Furthermore, the inflation component includes a push plate 61, a piston rod 62 is fixedly provided on one side of the push plate 61 corresponding to the movable block 5, a piston block 63 is fixedly provided on one end of the piston rod 62, an air cavity 52 is opened inside the movable block 5, the piston block 63 is slidably arranged along the inside of the corresponding air cavity 52, the push plate 61 is fixedly connected to the extrusion rod 6, and when the piston rod 62 moves in a direction away from the inner hose 42, the gas inside the air cavity 52 is filled into the interior of the airbag 72.

[0048] It should be noted that the extrusion rod 6 and the second piston rod 62 are both controlled by the push plate 61 . When the second piston rod 62 moves toward the direction close to the inner hose 42 , the gas inside the airbag 72 is sucked into the air cavity 52 .

[0049] Furthermore, an air channel 53 is opened inside the movable block 5 , and the air channel 53 is located outside the air cavity 52 , and one end of the air cavity 52 away from the inner hose 42 is connected to one end of the air channel 53 .

[0050] It should be noted that when the piston block 63 is located in the middle of the air cavity 52, it is the original point. When the piston rod 62 moves away from the inner hose 42, after the piston block 63 passes the original point, the piston rod 62 continues to move to perform the exhaust action, and the gas at the connection between the piston block 63 and the airway 53 is discharged to the airway 53, the hollow tube 71 and the airbag 72 in sequence. When the piston rod 62 moves close to the inner hose 42, after the piston block 63 passes the original point, the piston rod 62 continues to move to perform the pumping action, and the gas inside the airbag 72 is pumped back into the air cavity 52.

[0051] In this embodiment, the implementation scenario is specifically as follows: when the two groups of extrusion rods 6 squeeze the inner hose 42, the piston block 63 passes the original point and draws the gas inside the airbag 72 back into the air cavity 52, causing the airbag 72 to shrink and make room. The inner hose 42 is squeezed by the two groups of extrusion rods 6, and the other two sides corresponding to the airbag 72 bulge outward. When the two groups of extrusion rods 6 are retracted, after the piston block 63 passes the original point, the piston rod 2 62 continues to move to perform the exhaust action, and the gas at the connection between the piston block 63 and the airway 53 is discharged to the airbag 72 in turn. The expanded airbag 72 squeezes the bulge at the inner hose 42, restores it to its original position, and removes the adhesion effect between the inner walls of the inner hose 42.

[0052] Refer to the instruction manual Figures 8 to 10 The length of the connecting tube 4 is lengthened because the space outside the main body 1 is limited when the mounting block 2 is used as the connection point. Connecting multiple reagent tubes 3 requires lengthening the connecting tube 4 to increase the distance between the reagent tubes 3 and the main body 1 to obtain a larger space. In order to solve this problem, the following technical solution is also provided: the diameter of the main body 1 is enlarged to twice the original diameter, so that the distance between two adjacent groups of mounting blocks 2 along the circumferential direction is increased to twice.

[0053] It should be noted that by increasing the diameter of the main body 1, a larger space is obtained on the outside of the main body 1, and the same number of reagent tubes 3 as in the above scheme have more placement space, thereby shortening the length of the connecting tube 4 or directly eliminating the connecting tube 4 to avoid more high-concentration liquid reagents sticking to the inner wall of the connecting tube 4.

[0054] However, after increasing the diameter of the main body 1, the contact area between the piston end of the piston rod 11 and the high-concentration liquid reagent inside the main body 1 increases accordingly, causing the piston end of the piston rod 11 to be more easily adhered after contacting the high-concentration liquid reagent, and the increase in adhesion strength affects the vertical sliding of the piston rod 11.

[0055] Furthermore, a piston block 8 is fixedly provided at one end of the piston rod 11, and the piston block 8 is slidably arranged inside the main body 1, and an air guide pipe 81 runs through the interior of the piston block 8, the input end of the air guide pipe 81 is located at the top end of the piston rod 11, and the output end of the air guide pipe 81 is located at the bottom end of the piston block 8.

[0056] It should be noted that pure gas is introduced into the main body 1 through the air duct 81 to form an "air wall" at the bottom end of the piston block 8, and the piston block 8 is isolated from the high-concentration liquid reagent at the bottom by the "air wall".

[0057] Furthermore, a connecting block 82 is fixedly provided at the bottom end of the air guide tube 81, a rubber plate 83 is fixedly provided at the bottom of the piston block 8, and the connecting block 82 is fixedly connected to the middle part of the rubber plate 83, a groove 84 is provided at the bottom of the piston block 8, and an air hole 831 is provided in the middle part of the rubber plate 83, and the middle part of the rubber plate 83 is pulled upward by the air guide tube 81 and enters the interior of the groove 84.

[0058] It should be noted that when the middle part of the rubber plate 83 protrudes downward, it is convenient for the piston rod 11 to push downward. When the middle part of the rubber plate 83 protrudes upward, it can be away from the high-concentration liquid reagent at the bottom, making it less likely to stick.

[0059] In this embodiment, the implementation scenario is specifically as follows: by increasing the diameter of the main body 1, a larger space is obtained on the outside of the main body 1, thereby shortening the length of the connecting tube 4 or directly eliminating the connecting tube 4, so as to prevent more high-concentration liquid reagents from sticking to the inner wall of the connecting tube 4. Before the piston rod 11 is pushed downward, the middle part of the rubber plate 83 protrudes downward, and pure gas is introduced into the inside of the main body 1 through the air guide tube 81, forming an "air wall" at the bottom end of the piston block 2 8. When the piston rod 11 is pushed downward, the middle part of the rubber plate 83 protrudes upward, and the high-concentration liquid reagent entering the main body 1 is pushed through the "air wall", which can keep the piston block 2 8 away from the high-concentration liquid reagent at its bottom, making it less likely to stick.

[0060] Working principle:

[0061] 1. Squeeze the reagent tube 3 with your fingers to squeeze the liquid reagent in the reagent tube 3 into the connecting tube 4, and then use the mounting block 2 to allow the liquid reagent to enter the main tube 1. When part of the high-concentration liquid reagent sticks to the inner wall of the connecting tube 4, move the movable block 5 to the upper end of the reagent tube 3, and press the two sets of squeezing rods 6 to make them close to each other, so that the inner hose 42 can be squeezed from both sides, and then move the movable block 5 downward to make the squeezing rods 6 push the residual liquid reagent in the inner hose 42 to flow downward until the liquid reagent reaches the mounting block 2 and enters the main tube 1.

[0062] Second, when the squeezing rod 6 removes the squeezing force, the inner walls of the inner hose 42 on both sides corresponding to each other are stuck together by the high-concentration liquid reagent, and the inner walls of the inner hose 42 on both sides that are stuck together can be separated by the reset mechanism 7.

[0063] 3. By increasing the diameter of the main body 1, a larger space is obtained outside the main body 1, and the same number of reagent tubes 3 as in the above scheme have more placement space, thereby shortening the length of the connecting tube 4 or directly eliminating the connecting tube 4 to prevent more high-concentration liquid reagents from sticking to the inner wall of the connecting tube 4.

[0064] Fourth, by increasing the diameter of the main body 1, a larger space is obtained outside the main body 1, thereby shortening the length of the connecting tube 4 or directly eliminating the connecting tube 4, so as to prevent more high-concentration liquid reagents from sticking to the inner wall of the connecting tube 4. Before the piston rod 11 is pushed downward, the middle part of the rubber plate 83 protrudes downward, and pure gas is introduced into the main body 1 through the air guide pipe 81, forming an "air wall" at the bottom end of the piston block 2 8. When the piston rod 11 is pushed downward, the middle part of the rubber plate 83 protrudes upward, and the high-concentration liquid reagent entering the main body 1 is pushed through the "air wall", so that the piston block 2 8 can be kept away from the high-concentration liquid reagent at its bottom.

[0065] In order to reduce the loss of liquid reagents caused by adsorption on the inner wall of the connecting tube 4 during transportation, the prior art sets the connection position between the reagent tube 3 and the main body 1 at the lower part of the main body 1. When the required types of liquid reagents are large, the number of reagent tubes 3 set increases. Since the connection position between the reagent tube 3 and the main body 1 has been determined, and the number of reagent tubes 3 is limited by the outer area of ​​the main body 1, the following two situations occur: one: the diameter of the main body 1 remains unchanged, and the need to connect multiple reagent tubes 3 on the outside of the main body 1 is met by increasing the distance between the reagent tube 3 and the main body 1. In this case, the length of the connecting tube 4 between the reagent tube 3 and the main body 1 increases; two: the distance between the reagent tube 3 and the main body 1 remains unchanged, and the outer area of ​​the main body 1 is enlarged by increasing the diameter of the main body 1, so that more reagent tubes 3 can be connected at the determined connection position. In this case, the operating space inside the main body 1 is increased.

[0066] When a high-concentration liquid reagent is used for operation, in the first case mentioned above, part of the high-concentration liquid reagent will stick to the inner wall of the reagent tube 3, resulting in insufficient dosage of the liquid reagent introduced into the main body 1. In the second case mentioned above, due to the increase in the operating space inside the main body 1, it is necessary to increase the dosage of the required liquid reagent, which increases the viscosity inside the main body 1, making it difficult to squeeze out the liquid reagent after the operation. Therefore, the prior art does not require a machine for nucleic acid extraction tubes. When a large number of liquid reagents with high viscosity are used for operation, there will be a problem of insufficient dosage of the liquid reagent introduced into the main body 1 or difficulty in squeezing out the liquid reagent after the operation inside the main body 1. The present invention solves this problem through the above-mentioned scheme.

[0067] Refer to the instruction manual Fig.11 The present invention also provides a method for extracting nucleic acid without a machine, comprising the following steps:

[0068] S1: Prepare biological samples and send the biological samples into the main body 1;

[0069] S2: squeeze one of the liquid reagents into the main body 1 through one of the groups of reagent tubes 3;

[0070] S3: Push the liquid reagent adhered to the connecting tube 4 into the main tube 1 through the squeezing rod 6;

[0071] S4: squeeze another liquid reagent into the main body 1 through another set of reagent tubes 3, and repeat step S3;

[0072] S5: Repeat steps S2-S4 to perform nucleic acid extraction on the biological sample.

[0073] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the present invention. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention.

Claims

1. A nucleic acid extraction tube that does not require a machine, comprising a main body (1), a piston rod (11) is slidably provided inside the main body (1), an operating tube (12) is provided at the bottom end of the main body (1), a mounting block (2) is provided on the outer side of the lower part of the main body (1), and a plurality of groups of reagent tubes (3) are provided around the outer side of the main body (1), characterized in that: A connecting pipe (4) is connected between the mounting block (2) and the reagent tube (3); a movable block (5) penetrates the outer side of the connecting pipe (4), and the movable block (5) is slidably arranged along the axial direction of the connecting pipe (4); The connecting pipe (4) comprises an outer sleeve (41), an inner hose (42) passing through the inner sleeve (41), an extrusion rod (6) is mounted on the movable block (5), and two groups of extrusion rods (6) are arranged on both sides corresponding to the inner hose (42), and when the movable block (5) drives the corresponding extrusion rods (6) to move toward the mounting block (2), the two groups of extrusion rods (6) approach each other so that the reagent in the inner hose (42) flows toward the mounting block (2); The inner wall of the outer sleeve (41) is provided with a guide groove (43), and guide blocks (51) are fixedly provided on both sides of the movable block (5), and the guide blocks (51) are slidably arranged along the inside of the corresponding guide groove (43); A reset mechanism (7) is installed on the inner wall of the outer sleeve (41). When the two groups of squeeze rods (6) squeeze the inner hose (42), a protrusion is generated so that the inner hose (42) moves away from the squeezed position. The reset mechanism (7) is arranged corresponding to the protrusion. The reset mechanism (7) is used to reset the protrusion. The resetting mechanism (7) comprises a hollow tube (71), an airbag (72) is mounted on the hollow tube (71), the movable block (5) and the airbag (72) are connected via the hollow tube (71), an inflatable component is mounted inside the movable block (5), and when the extrusion rod (6) moves in a direction away from the inner hose (42), air is inflated into the airbag (72) via the inflatable component.

2. The machine-free nucleic acid extraction tube according to claim 1, characterized in that: The inflation assembly comprises a push plate (61), a piston rod 2 (62) is fixedly provided on one side of the push plate (61) corresponding to the movable block (5), a piston block 1 (63) is fixedly provided on one end of the piston rod 2 (62), an air cavity (52) is provided inside the movable block (5), the piston block 1 (63) is slidably arranged along the inside of the corresponding air cavity (52), the push plate (61) is fixedly connected to the extrusion rod (6), and when the piston rod 2 (62) moves in a direction away from the inner hose (42), the gas inside the air cavity (52) is filled into the inside of the airbag (72).

3. A machine-free nucleic acid extraction tube according to claim 2, characterized in that: An air channel 1 (53) is provided inside the movable block (5), and the air channel 1 (53) is located outside the air cavity (52), and an end of the air cavity (52) away from the inner hose (42) is connected to an end of the air channel 1 (53).

4. The machine-free nucleic acid extraction tube according to claim 1, characterized in that: The diameter of the main pipe (1) is enlarged to twice the original diameter, so that the distance between two adjacent groups of mounting blocks (2) along the circumferential direction is increased to twice.

5. The machine-free nucleic acid extraction tube according to claim 4, characterized in that: A piston block 2 (8) is fixedly provided at one end of the piston rod 1 (11), and the piston block 2 (8) is slidably arranged inside the main pipe body (1), and an air guide pipe (81) runs through the interior of the piston block 2 (8), the input end of the air guide pipe (81) is located at the top end of the piston rod 1 (11), and the output end of the air guide pipe (81) is located at the bottom end of the piston block 2 (8).

6. The machine-free nucleic acid extraction tube according to claim 5, characterized in that: A connecting block (82) is fixedly provided at the bottom end of the air guide tube (81), a rubber plate (83) is fixedly provided at the bottom of the second piston block (8), and the connecting block (82) and the middle part of the rubber plate (83) are fixedly connected, a groove (84) is provided at the bottom of the second piston block (8), and an air hole (831) is provided in the middle part of the rubber plate (83), and the middle part of the rubber plate (83) is pulled upward by the air guide tube (81) and enters the interior of the groove (84).

7. An extraction method using the machine-free nucleic acid extraction tube according to claim 1, characterized in that: The steps include: S1: preparing a biological sample and sending the biological sample into the main body (1); S2: squeezing one of the liquid reagents into the main body (1) through one of the groups of reagent tubes (3); S3: pushing the liquid reagent adhered to the connecting tube (4) into the main tube (1) through the squeezing rod (6); S4: another liquid reagent is squeezed into the main body (1) through another set of reagent tubes (3), and step S3 is repeated; S5: Repeat steps S2-S4 to perform nucleic acid extraction on the biological sample.

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