A method for planting isatis root based on artificial light source

Abstract

The present invention discloses a method for cultivating Radix Isatidis using artificial light sources. The method comprises spraying a nutrient solution onto the roots of Radix Isatidis seedlings using an atomizing cultivation device, and controlling the cultivation conditions during the seedling, growth, and maturation stages to produce mature Radix Isatidis. This invention, for the first time, proposes a method for cultivating Radix Isatidis using artificial light sources through atomizing cultivation. By regulating the cultivation process and combining it with a specific culture solution formulation, the cultivation cycle is significantly shortened while ensuring product quality.

Description

Technical Field

[0001] The invention belongs to the technical field of soilless cultivation of traditional Chinese medicine, and particularly relates to a planting method of isatis root based on artificial light source. Background Art

[0002] Isatis indigotica, a common medicinal plant, is a Chinese herbal remedy. It clears heat and detoxifies, reduces inflammation, and relieves coughs. It is used to treat coughs, sore throats, tonsillitis, oral ulcers, bad breath, and periodontitis. Due to its broad-spectrum antimicrobial properties, it is also effective in treating bacterial infections, making it a commonly used Chinese herbal remedy. Its medicinal components possess various physiological activities, including antibacterial, anti-inflammatory, analgesic, and immunomodulatory. They effectively clear heat and detoxify, relieving fever, cough, sore throat, and muscle pain.

[0003] In recent years, as people's demand for Chinese medicinal materials has gradually increased, however, since isatis root is an annual herb with a growing period of nine months, most of it is cultivated artificially; the yield is low and depends on the weather.

[0004] At present, the cultivation of Isatis root relies on natural light cultivation, which has a long cycle and low efficiency. Summary of the Invention

[0005] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the abstract and title of this application to avoid obscuring the purpose of this section, the abstract and the title of the invention, and such simplifications or omissions should not be used to limit the scope of the present invention.

[0006] The present invention is proposed in view of the above problems and / or the problems in the prior art.

[0007] Therefore, the purpose of the present invention is to overcome the deficiencies in the prior art and provide a method for planting Radix Isatidis based on artificial light sources.

[0008] In order to solve the above technical problems, the present invention provides the following technical solutions: a planting method of Radix Isatidis based on artificial light source, comprising:

[0009] The nutrient solution is sprayed on the roots of the Radix Isatidis seedlings by an atomizing cultivation device, and the cultivation conditions in the seedling stage, the growth stage, and the maturity stage are controlled to obtain mature Radix Isatidis;

[0010] The culture conditions during the seedling stage are as follows: the red-blue light ratio is 4:1, and the light intensity is 200-250 μmol / m 2 / s, the light duration is 12~14 hours / day, which is called the light period; the light-off time is 10~12 hours / day, which is called the dark period, the photoperiod is 14~18 hours / day, and the growth cycle is 15 days;

[0011] The culture conditions during the growth period were: a red-blue light ratio of 5:1, and a light intensity of 200-250 μmol / m 2 / s, the light duration is 14-18 hours / day, which is called the light period; the lights are turned off for 6-10 hours / day, which is called the dark period. The light cycle is 14-18 hours / day, and the growth cycle is 20 days;

[0012] The culture conditions during the mature stage were as follows: the red-blue light ratio was 4:1, and the light intensity was 200-250 μmol / m 2 / s, the lighting time is 20~22 hours / day, which is called the light period; the lights are turned off for no more than 2~4 hours / day, which is called the dark period. The light period is 20~22 hours / day, and the growth cycle is 15 days.

[0013] As a preferred embodiment of the planting method of the present invention, the isatis root seedlings include isatis root seedlings that are free of pests and diseases and mechanical damage, have strong and neat root systems, and are 5 to 8 cm in height.

[0014] As a preferred embodiment of the planting method of the present invention, the roots of the isatis seedlings are planted in a cultivation device.

[0015] As a preferred embodiment of the planting method of the present invention, the CO2 concentration during the seedling stage is 800-1000 ppm, the relative humidity is 50-80%, and the culture temperature is 20-30°C.

[0016] As a preferred embodiment of the planting method of the present invention, the CO2 concentration during the growth period is 800-1000 ppm, the relative humidity is 50-80%, and the culture temperature is 20-30°C.

[0017] As a preferred embodiment of the planting method of the present invention, the CO2 concentration during the cultivation at the maturity stage is 800-1000 ppm, the relative humidity is 50-80%, and the cultivation temperature is 20-30°C.

[0018] As a preferred embodiment of the planting method of the present invention, the nutrient solution comprises the following components per liter of nutrient solution:

[0019] 5Ca(NO3)2.NH4.NO3.10H2O 470mg / L; KNO3390mg / L; EDTA-Fe38mg / L; MgSO4.7H2O200mg / L; KH2PO471mg / L; NH4H2PO444.6mg / L; H3BO32.92mg / L; MnSO4.4H2O 2.08mg / L; ZnSO4.7H2O 0.24mg / L; CuSO4.5H2O 0.06mg / L; (NH4)6Mo7O 24 .4H2O 0.03mg / L;

[0020] The solvent is distilled water, and the pH of the nutrient solution is 5.5~6.5.

[0021] As a preferred embodiment of the planting method of the present invention, the atomizing cultivation device comprises a nutrient solution tank (100), a water pump (200), a controller (300), a solenoid valve (400), a planting plate (500), and a cross atomizing nozzle (600) arranged under the planting plate (500); wherein,

[0022] Automatically controlling the spraying is achieved through a water pump (200), a controller (300), a solenoid valve (400), and a cross-atomizing nozzle (600);

[0023] A high-density polystyrene board with holes is selected as the planting board (500), and a cross-shaped atomizing nozzle (600) is hung upside down on the planting board at intervals of 40 cm to ensure that the entire internal space is evenly atomized during spraying.

[0024] As a preferred embodiment of the planting method of the present invention, the flow rate of the atomizing nozzle is 20-50 L / H, and the working pressure is 1-2.0 kg;

[0025] Spray frequency: spray duration / spray interval is 15~30s / 5min

[0026] Beneficial effects of the present invention:

[0027] (1) The present invention proposes for the first time a method for cultivating Radix Isatidis by atomization using artificial light sources. By regulating the cultivation process and combining it with a specific culture solution formula, the cultivation cycle is greatly shortened while ensuring the quality of the product.

[0028] In view of the fact that the current growth cycle of Isatis indigotica is 9 months, the present invention cultivates Isatis indigotica by aeroponic cultivation in a plant factory with artificial light sources, with a total growth period of 50 days, which greatly shortens the planting cycle.

[0029] (2) The present invention adjusts different light conditions during the growth and maturation periods of Radix Isatidis, thereby achieving the purpose of rapid cultivation and maturation while ensuring the activity of Radix Isatidis. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort. Among them:

[0031] Figure 1 This is a diagram of an atomization cultivation device in an embodiment of the present invention. DETAILED DESCRIPTION

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0033] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0034] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive of other embodiments.

[0035] In the embodiment of the present invention, the red-blue light ratio uses artificial light source LED light to replace sunlight. The number of red light sources and the number of blue light sources are the red-blue light ratio. Specifically, the illumination light source is formed by combining a monochromatic red light emitting unit (peak wavelength 660 nm) and a monochromatic blue light emitting unit (peak wavelength 455 nm).

[0036] Example 1

[0037] This embodiment provides a method for planting Radix Isatidis based on artificial light sources, the main steps of which are:

[0038] (1) Prepare the nutrient solution for soilless vertical cultivation of Isatis root:

[0039] Each liter of nutrient solution contains the following components:

[0040] 5Ca(NO3)2.NH4.NO3.10H2O 470mg / L; KNO3390mg / L; EDTA-Fe38mg / L; MgSO4.7H2O200mg / L; KH2PO471mg / L; NH4H2PO444.6mg / L; H3BO32.92mg / L; MnSO4.4H2O 2.08mg / L; ZnSO4.7H2O 0.24mg / L; CuSO4.5H2O 0.06mg / L; (NH4)6Mo7O 24 .4H2O 0.03mg / L;

[0041] The solvent is distilled water.

[0042] (2) Planting the Isatis indigotica seedlings with no pests, diseases or mechanical damage, strong and neat root systems, and a height of 5 to 8 cm in a cultivator and cultivating them in an atomized cultivation device;

[0043] Among them, the atomization cultivation device Figure 1 , comprising a nutrient solution pool (100), a water pump (200), a controller (300), a solenoid valve (400), a planting plate (500), and a cross-atomizing nozzle (600) disposed under the planting plate (500);

[0044] Automatically controlling the spraying is achieved through a water pump (200), a controller (300), a solenoid valve (400), and a cross-atomizing nozzle (600);

[0045] A high-density polystyrene board with holes is selected as a planting board (500), and a cross-shaped atomizing nozzle (600) is hung upside down on the planting board at intervals of 40 cm to ensure that the entire internal space is evenly atomized during spraying;

[0046] When planting, wrap the plant with a sponge and place it in the planting hole;

[0047] The flow rate of the atomizing nozzle is 50L / H and the working pressure is 2.0kg;

[0048] The spray frequency (spray duration / spray interval) is 15s / 5min.

[0049] (3) Spraying the nutrient solution onto the roots of the Radix Isatidis seedlings through an atomizing cultivation device, controlling the culture conditions during the seedling stage, the growth stage, and the maturity stage to obtain mature Radix Isatidis;

[0050] The culture conditions during the seedling stage were as follows: the red-blue light ratio was 4:1, and the light intensity was 250 μmol / m 2 / s, light duration of 14 hours / day, called the light period; lights-off time of 10 hours / day, called the dark period, photoperiod of 14 hours / day, growth period of 15 days, CO2 concentration of 800ppm, relative humidity of 80%, and culture temperature of 25℃;

[0051] The culture conditions during the growth period were: a red-blue light ratio of 5:1, and a light intensity of 250 μmol / m 2 / s, light duration of 18 hours / day, called the light period; lights off for 6 hours / day, called the dark period, photoperiod of 18 hours / day, growth period of 20 days, CO2 concentration of 800ppm, relative humidity of 80%, and culture temperature of 25℃;

[0052] The culture conditions during the mature stage were as follows: the red-blue light ratio was 4:1, and the light intensity was 250 μmol / m 2 / s, the lighting time is 22 hours / day, which is called the light period; the lights are turned off for no more than 2 hours / day, which is called the dark period. The photoperiod is 22 hours / day, the growth cycle is 15 days, the CO2 concentration is 800ppm, the relative humidity is 80%, and the culture temperature is 25℃.

[0053] Comparative Example 1

[0054] The difference between this comparative example and Example 1 lies in step (3). Other conditions are the same as those in Example 1. The specific difference lies in:

[0055] (3) Spraying the nutrient solution onto the roots of the Radix Isatidis seedlings through an atomizing cultivation device, controlling the culture conditions during the seedling stage, the growth stage, and the maturity stage to obtain mature Radix Isatidis;

[0056] The culture conditions during the seedling stage were as follows: the red-blue light ratio was 3:2, and the light intensity was 250 μmol / m 2 / s, light duration of 14 hours / day, called the light period; lights-off time of 10 hours / day, called the dark period, photoperiod of 14 hours / day, growth period of 15 days, CO2 concentration of 800ppm, relative humidity of 80%, and culture temperature of 25℃;

[0057] The culture conditions during the growth period were: a red-blue light ratio of 3:2, and a light intensity of 250 μmol / m 2 / s, light duration of 18 hours / day, called the light period; lights off for 6 hours / day, called the dark period, photoperiod of 18 hours / day, growth period of 20 days, CO2 concentration of 800ppm, relative humidity of 80%, and culture temperature of 25℃;

[0058] The culture conditions during the mature stage were as follows: the red-blue light ratio was 3:2, and the light intensity was 250 μmol / m 2 / s, the lighting time is 22 hours / day, which is called the light period; the lights are turned off for no more than 2 hours / day, which is called the dark period. The photoperiod is 22 hours / day, the growth cycle is 15 days, the CO2 concentration is 800ppm, the relative humidity is 80%, and the culture temperature is 25℃.

[0059] Comparative Example 2

[0060] The difference between this comparative example and Example 1 lies in step (3). Other conditions are the same as those in Example 1. The specific difference lies in:

[0061] The nutrient solution is sprayed on the roots of the Radix Isatidis seedlings by an atomizing cultivation device, and the cultivation conditions in the seedling stage, the growth stage, and the maturity stage are controlled to obtain mature Radix Isatidis;

[0062] The culture conditions during the seedling stage were as follows: the red-blue light ratio was 5:1, and the light intensity was 250 μmol / m 2 / s, light duration of 14 hours / day, called the light period; lights-off time of 10 hours / day, called the dark period, photoperiod of 14 hours / day, growth period of 15 days, CO2 concentration of 800ppm, relative humidity of 80%, and culture temperature of 25℃;

[0063] The culture conditions during the growth period were: a red-blue light ratio of 5:1, and a light intensity of 250 μmol / m 2 / s, light duration of 18 hours / day, called the light period; lights off for 6 hours / day, called the dark period, photoperiod of 18 hours / day, growth period of 20 days, CO2 concentration of 800ppm, relative humidity of 80%, and culture temperature of 25℃;

[0064] The culture conditions during the mature stage were as follows: the red-blue light ratio was 5:1, and the light intensity was 250 μmol / m 2 / s, the lighting time is 22 hours / day, which is called the light period; the lights are turned off for no more than 2 hours / day, which is called the dark period. The photoperiod is 22 hours / day, the growth cycle is 15 days, the CO2 concentration is 800ppm, the relative humidity is 80%, and the culture temperature is 25℃.

[0065] Comparative Example 3

[0066] The difference between this comparative example and Example 1 lies in step (3). Other conditions are the same as those in Example 1. The specific difference lies in:

[0067] The nutrient solution is sprayed on the roots of the Radix Isatidis seedlings by an atomizing cultivation device, and the cultivation conditions in the seedling stage, the growth stage, and the maturity stage are controlled to obtain mature Radix Isatidis;

[0068] The culture conditions during the seedling stage were as follows: the red-blue light ratio was 3:2, and the light intensity was 250 μmol / m 2 / s, light duration of 14 hours / day, called the light period; lights-off time of 10 hours / day, called the dark period, photoperiod of 14 hours / day, growth period of 15 days, CO2 concentration of 800ppm, relative humidity of 80%, and culture temperature of 25℃;

[0069] The culture conditions during the growth period were: a red-blue light ratio of 3:2, and a light intensity of 250 μmol / m 2 / s, light duration of 18 hours / day, called the light period; lights off for 6 hours / day, called the dark period, photoperiod of 18 hours / day, growth period of 20 days, CO2 concentration of 800ppm, relative humidity of 80%, and culture temperature of 25℃;

[0070] The culture conditions during the mature stage were as follows: the red-blue light ratio was 3:2, and the light intensity was 250 μmol / m 2 / s, the lighting time is 23 hours / day, which is called the light period; the lights are turned off for no more than 1 hour / day, which is called the dark period. The photoperiod is 23 hours / day, the growth cycle is 15 days, the CO2 concentration is 800ppm, the relative humidity is 80%, and the culture temperature is 25℃.

[0071] The (R,S)-galactopyran content of the Radix Isatidis in the Examples and Comparative Examples of the present invention was determined according to high performance liquid chromatography (General Rule 0512). In the Examples and Comparative Examples of the present invention, the Radix Isatidis was cleaned, dried, and its dry weight was determined. The root length (the length from the base of the leaf disc to the bottom of the root, accurate to 0.01 cm) was measured.

[0072] The results are shown in Table 1.

[0073] Table 1

[0074] Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 (R,S)-Co-Isopropylamine (mg / g) 6.72 5.68 5.28 5.15 Dry weight (g) 21.24 18.43 16.36 21.42 Root length (cm) 31.13 18.68 19.88 31.22

[0075] As can be seen from Table 1, the artificial light-based cultivation method of the present invention, by regulating the cultivation process and combining a specific culture solution formula, greatly shortens the cultivation cycle while ensuring product cultivation quality. The current growth cycle of isatis root is 9 months. The present invention cultivates isatis root through aeroponic cultivation in a plant factory with artificial light, with a total growth period of 40 days, greatly shortening the cultivation cycle.

[0076] At the same time, the (R,S)-coitrin content of the prepared product meets the requirements;

[0077] The ratio of red and blue light in Comparative Examples 1 and 2 is inappropriate, and the obtained effect is also poor. This may be because during the cultivation process, blue light promotes the growth of Isatis root, and red light helps Isatis root to dormant and shed leaves, and promotes maturity. When the ratio of the two is inappropriate, the expected goal of our invention cannot be achieved.

[0078] In Comparative Example 3, during the cultivation process at the mature stage, the illumination time was inappropriate (too long), and the expected goal could not be achieved, and the effective content was reduced.

[0079] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, and all of these should be included in the scope of the present invention.

Claims

1. A method for planting Radix Isatidis based on artificial light source, characterized by: include, The nutrient solution is sprayed on the roots of the Radix Isatidis seedlings by an atomizing cultivation device, and the cultivation conditions in the seedling stage, the growth stage, and the maturity stage are controlled to obtain mature Radix Isatidis; The culture conditions during the seedling stage are as follows: the red-blue light ratio is 4:1, and the light intensity is 200-250 μmol / m 2 / s, light duration of 14 hours / day, called the light period; lights off for 10 hours / day, called the dark period, the photoperiod is 14 hours / day, the growth cycle is 15 days, the CO2 concentration in the culture is 800~1000ppm, the relative humidity is 50~80%, and the culture temperature is 20~30℃; The culture conditions during the growth period were: a red-blue light ratio of 5:1, and a light intensity of 200-250 μmol / m 2 / s, light duration of 14-18 hours / day, called the light period; lights-off time of 6-10 hours / day, called the dark period, light period of 14-18 hours / day, growth period of 20 days, CO2 concentration of culture of 800-1000ppm, relative humidity of 50-80%, culture temperature of 20-30℃; The culture conditions during the mature stage were as follows: the red-blue light ratio was 4:1, and the light intensity was 200-250 μmol / m 2 / s, the light duration is 20-22 hours / day, which is called the light period; the lights are turned off for no more than 2-4 hours / day, which is called the dark period, the light period is 20-22 hours / day, the growth period is 15 days, the CO2 concentration in the culture is 800-1000ppm, the relative humidity is 50-80%, and the culture temperature is 20-30℃; The nutrient solution comprises the following components per liter of nutrient solution: 5Ca(NO3)2.NH4.NO3.10H2O 470mg / L; KNO3390mg / L; EDTA-Fe38mg / L; MgSO4.7H2O 200mg / L; KH2PO471mg / L; NH4H2PO444.6mg / L; H3BO32.92mg / L; MnSO4.4H2O 2.08mg / L; ZnSO4.7H2O0.24mg / L; CuSO4.5H2O 0.06mg / L; (NH4)6Mo7O 24 .4H2O 0.03mg / L; the solvent is distilled water, and the pH of the nutrient solution is 5.5~6.

5.

2. The planting method according to claim 1, wherein: The isatis root seedlings include isatis root seedlings that are free of diseases, insect pests and mechanical damage, have strong and neat root systems and are 5 to 8 cm in height.

3. The planting method according to claim 1 or 2, characterized in that: The roots of the isatis root seedlings are planted in a cultivation device.

4. The planting method according to claim 1, wherein: The atomizing cultivation device comprises a nutrient solution pool (100), a water pump (200), a controller (300), a solenoid valve (400), a planting plate (500), and a cross atomizing nozzle (600) arranged under the planting plate (500); wherein, Automatically controlling the spraying is achieved through a water pump (200), a controller (300), a solenoid valve (400), and a cross-atomizing nozzle (600); A high-density polystyrene board with holes is selected as the planting board (500), and a cross-shaped atomizing nozzle (600) is hung upside down on the planting board at intervals of 40 cm to ensure that the entire internal space is evenly atomized during spraying.

5. The planting method according to claim 4, characterized in that: The flow rate of the atomizing nozzle is 20~50L / H, and the working pressure is 1~2.0kg; Spray frequency: Spray duration / spray interval is 15~30s / 5min.

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