Sinapic acid plant growth regulator and its application

Abstract

The present invention provides a sinapinic acid plant growth regulator and its application. The sinapinic acid plant growth regulator is used to alleviate the continuous cropping disorder of ramie plants. The raw materials include, by weight, 10 to 30 parts of sinapinic acid and 1 to 3 parts of a slow-release solvent. The pH value of the sinapinic acid plant growth regulator is 5.0 to 7.0. The sinapinic acid plant growth regulator provided in this application reduces the damage of continuous cropping to ramie plants and improves related indicators such as plant agronomic traits, root vitality, chlorophyll content, and antioxidant system enzyme activity. This preliminarily reveals a new cultivation technology for alleviating soil damage caused by continuous cropping of ramie.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and in particular relates to a sinapinic acid plant growth regulator and an application thereof. Background Art

[0002] Sinapinic acid, 3,5-dimethoxy-4-hydroxycinnamic acid, chemical formula C 11 H 12 O5, a light yellow powder, is widely distributed in the plant kingdom and exists in spices, fruits, vegetables, grains and oil crops. Literature has reported that low concentrations of 0.1 to 1 mM sinapic acid can promote seed germination and seedling growth. However, there are currently no relevant literature and patents on sinapic acid alleviating plant continuous cropping problems.

[0003] Continuous cropping disorder refers to abnormal crop growth and development caused by the continuous cultivation of the same crop or closely related crops on the same soil. Symptoms generally include poor growth and development, reduced yield and quality, and in extreme cases, localized seedling death, no seedling emergence, or weak seedling emergence. Most affected plants experience browning of the roots, reduced branching, low vitality, and a narrow distribution range, resulting in a reduced ability to absorb water and nutrients. Ramie, a perennial herb with perennial roots, is an important textile fiber crop in my country. Continuous cropping disorder is a common problem in continuous cropping of ramie in greenhouses, leading to a serious decline in ramie production. Therefore, using sinapinic acid plant growth regulators to address the problem of continuous cropping of ramie is of great practical significance. Summary of the Invention

[0004] The technical problem to be solved by the present invention is that continuous cropping obstacles lead to a serious decline in ramie production, overcomes the deficiencies and defects mentioned in the above background technology, and provides a sinapinic acid plant growth regulator and its application.

[0005] In order to solve the above technical problems, the technical solution proposed by the present invention is:

[0006] A sinapinic acid plant growth regulator is used for alleviating the continuous cropping disorder of ramie plants. The raw materials comprise, by mass, 10 to 30 parts of sinapinic acid and 1 to 3 parts of a slow-release solvent. The pH value of the sinapinic acid plant growth regulator is 5.0 to 7.0.

[0007] Sinapic acid has a pKa value of 8.2, which means that when the pH of a solution is below 8.2, the thio group in the sinapic acid molecule becomes a negative ion, significantly increasing its solubility in acidic environments. This property allows the solubility of sinapic acid to be controlled by adjusting the pH of the solution during organic synthesis and drug development, thereby optimizing reaction conditions or improving product purity.

[0008] Preferably, the concentration of sinapinic acid is 200 mg·L -1 ~300mg·L -1 .

[0009] Sinapic acid promotes plant growth at low concentrations, but inhibits it at high concentrations. Sinapic acid has a complex relationship with auxin and abscisic acid. Sinapic acid and its derivatives can affect the activity and stability of auxin and abscisic acid in plants through glycosylation, as well as their transport and distribution. These interactions together constitute a complex hormone regulatory network in plants, which has an important regulatory effect on plant growth and development. Within the range selected by the present invention, sinapic acid has the best regulatory effect on ramie, with ramie seedlings growing optimally and also significantly alleviating the yellowing of ramie leaves.

[0010] Preferably, the soil used for the continuous cropping barrier is sandy clay loam in which ramie crops have been continuously planted for 3-10 years.

[0011] Preferably, the sustained-release solvent is an ethanol solution with a volume concentration of 50-60%, which effectively improves the solubility of sinapinic acid and maintains its stability.

[0012] Ethanol solutions between 50% and 60% can enhance the solubility of sinapinic acid, prevent degradation, and provide antioxidant properties. The dissolution process of sinapinic acid in {EG(1)+water(2)} solution is driven by endothermicity and entropy. In 50% to 60% ethanol solutions, this process also conforms to thermodynamic laws, which is beneficial to the improvement of solubility.

[0013] Sinapic acid may degrade under certain conditions, particularly in strongly acidic or alkaline environments. A 50% to 60% ethanol solution provides a mild chemical environment, reducing the risk of sinapic acid degradation at extreme pH values.

[0014] Sinapic acid and its derivatives have certain antioxidant activity. Using an appropriate concentration of ethanol solution as a solvent can maintain these antioxidant properties, thereby achieving better results during extraction and application.

[0015] Furthermore, considering the cost-effectiveness, safety and convenience, these advantages make 50% to 60% ethanol solution an ideal choice for dissolving sinapinic acid.

[0016] Preferably, the physical and chemical properties of the soil used to treat continuous cropping obstacles include: pH 4.0-7.0; soil conductivity 80-200 S / cm; organic matter content 20-200 g / kg; hydrolyzable nitrogen content 100-200 mg / kg; available phosphorus content 20-200 mg / kg; and available potassium content 20-300 mg / kg. Selecting southern acidic soil for continuous cropping, combined with the sinapinic acid regulator of this application, can effectively alleviate continuous cropping obstacles.

[0017] Under the same technical concept, the present application also provides an application of a sinapinic acid plant growth regulator, which is used to alleviate the continuous cropping obstacle of ramie, specifically comprising the following steps:

[0018] (1) Cultivating ramie seedlings, and after the ramie seedlings have grown, irrigating the roots of the ramie seedlings with a sinapinic acid plant growth regulator, 50 to 100 mL each time, once every three days;

[0019] (2) After 30 days of root irrigation, the cultivation was completed and the plant height, stem diameter, SPAD value, total chlorophyll content, antioxidant enzyme activity (SOD, POD, CAT) and plant leaf transcriptome sequencing were analyzed.

[0020] Preferably, the height of the ramie plants during the root irrigation treatment is 1.5 to 2 cm; and the irrigation range of the root irrigation treatment is within a radius of 2 cm from the ramie roots.

[0021] Preferably, topdressing and pest control are performed during the root irrigation treatment, the fertilizer used for the topdressing includes Hoagland nutrient solution, and the objects of the pest control treatment include armyworms and / or board armyworms.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) The sinapinic acid plant growth regulator provided in this application reduces the damage of continuous cropping to ramie plants, improves the plant's agronomic traits, root vitality, chlorophyll content, antioxidant system enzyme activity and other related indicators, and preliminarily reveals a new cultivation technology to alleviate ramie continuous cropping soil;

[0024] (2) Provide an application method of the sinapinic acid plant growth regulator, which is simple to operate. After application, the enzyme activity and chlorophyll content of the ramie plants grown are significantly improved, the growth condition is good, the yield is increased, and it has a good market prospect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0026] Figure 1 are photos of the growth status of ramie plants in Example 1 and Comparative Example 1;

[0027] Figure 2 1 is a graph showing the regulation of enzyme activity in ramie plants by applying sinapinic acid concentration in Example 1 and Comparative Example 1;

[0028] Figure 3 This is a test chart of regulating the chlorophyll content of ramie plants by applying sinapinic acid concentration in Example 1 and Comparative Example 1;

[0029] Figure 4 This is a graph showing the transcriptome sequencing results of Example 1. DETAILED DESCRIPTION

[0030] To facilitate understanding of the present invention, the present invention will be described in more comprehensive and detailed form below in conjunction with the accompanying drawings and preferred embodiments. However, the protection scope of the present invention is not limited to the following specific embodiments.

[0031] Unless otherwise defined, all technical terms used hereinafter have the same meanings as those generally understood by those skilled in the art. The technical terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0032] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0033] The examples and comparative examples of this application were conducted in a greenhouse at the Institute of Bast and Hemp, Chinese Academy of Agricultural Sciences, in 2023. Located at 112°58'42" east longitude and 3°11'49" north latitude, the region enjoys a subtropical monsoon climate, boasting abundant rainfall, an average annual precipitation of 1361.6 mm, simultaneous rain and heat, four distinct seasons, and an average temperature of 17.2°C. The ramie (Boehmeria nivea L.) variety used for testing was 'Zhongzhu No. 1' (from the Institute of Bast and Hemp); sinapinic acid (SA) was purchased from Macklin.

[0034] The test soil is a sandy clay loam soil from a field where ramie has been grown for eight consecutive years. The basic physical and chemical properties are as follows:

[0035]

[0036]

[0037] Select plump, uniform seeds and, after 24 hours of germination, sow them in nutrient pots (10 cm x 10 cm) filled with test soil. When the seedlings have three leaves and one heart, transplant them into test pots filled with 10 kg of continuous cropping test soil. After the seedlings have grown, select 200 plants with consistent growth as test materials. Repeat this three times, and arrange them in completely random order.

[0038] Example 1:

[0039] The sinapinic acid plant growth regulator of this embodiment comprises, by weight, sinapinic acid and a slow-release solvent in a volume ratio of 10:1, and a solution pH of 5.0 to 7.0.

[0040] The application of a sinapinic acid plant growth regulator according to this embodiment comprises the following steps:

[0041] After the experimental ramie seedlings were grown, sinapic acid plant growth regulator was applied to all the plants except the control group. The sinapic acid concentrations in the sinapic acid plant growth regulator were 50, 100, 150, 200, 250, 300, and 400 mg·L -1 Each treatment was repeated 3 times, and the ramie seedlings were irrigated with 100 mL of water at 9:00 am each time, and the treatment was repeated every 3 days.

[0042] During the root irrigation treatment, top dressing and pest control are carried out. The fertilizer used for top dressing is Hogland nutrient solution. The targets of pest control include armyworms and / or board armyworms.

[0043] After the cultivation of ramie was completed, the growth traits of ramie were measured, including plant height, stem diameter, SPAD value, fresh weight and dry weight; the chlorophyll content was determined using the national standard method HJ897-2017; the plant antioxidant enzyme activity (SOD, POD, CAT enzyme activity) was tested using a Solebaugh test kit; and the total roots (cm), average root diameter (mm), total root surface area (cm2), total root surface area (cm2), total root volume (cm3), number of root tips, number of branches and number of intersections of the plants were analyzed using a root scanner.

[0044] Transcriptome Analysis: Total RNA was extracted from ramie leaves and reverse-transcribed into cDNA. The cDNA was end-repaired, A-tailed, and adapter-ligated to construct a library suitable for high-throughput sequencing. The library was sequenced by Shanghai Personal Biotechnology Co., Ltd. (Shanghai, China). The constructed library was sequenced using an Illumina HiSeq platform. The resulting raw data were quality-controlled and aligned with the Qingye ramie reference genome (https: / / www.ncbi.nlm.nih.gov / assembly / GCA_018132145.1) for annotation and identification of corresponding genes and functions. Significantly differentially expressed genes were then identified. Functional enrichment analysis of differentially expressed genes using Kyoto Encyclopedia of Genes and Genomes (KEGG) databases was performed, with p-values ​​less than 0.05.

[0045] Table 1 Test data of agronomic traits of ramie

[0046]

[0047]

[0048] The root growth data of ramie are shown in Table 2 below:

[0049] Table 2 Root growth data of ramie

[0050]

[0051] Through experimental research, it was found that Figure 1 As shown in the figure, for ramie seedlings in acidified continuous cropping soil, it can promote their growth, increase plant height and stem diameter, and increase ramie yield. Figure 1 (a), (b), (c) are photos of the plant height, leaf and stem diameter of ramie plants, respectively. When the sinapic acid concentration in the sinapic acid plant growth regulator was 200 mg·L -1 ~300mg·L -1 Within this range, the growth state of ramie seedlings is the best, and it also has a significant alleviating effect on the yellowing of ramie leaves.

[0052] like Figure 2 As shown, the plant growth regulator of this embodiment also significantly regulates the enzyme activity of ramie leaves. After the application of sinapinic acid, the SOD, POD, and CAT values ​​of the ramie plants first decrease and then tend to be stable, indicating that the growth state of the ramie plants is relatively good, the damage to the plants in the continuous cropping soil is gradually reduced, and it is beneficial to the growth and development of the plants.

[0053] like Figure 3 As shown in the figure, for continuous cropping ramie in facilities with serious continuous cropping soil, both SPAD value and total chlorophyll content showed an upward trend.

[0054] like Figure 4 As shown, transcriptome sequencing was performed, and the test results demonstrated increased expression of genes related to photosynthesis, glutamine, and flavonoid metabolism. Photosynthesis is directly linked to leaf greening, glutamine can promote lateral root growth, and flavonoid metabolism is a downstream product of exogenously applied sinapinic acid, which can promote the flavonoid metabolic pathway. The sequencing results demonstrate that the plant growth regulator of this example achieves overall trait regulation in ramie plants.

[0055] Comparative Example 1:

[0056] The comparative example uses 0mg·L -1 The sinapic acid solution was used as the control group (denoted as CK), i.e., deionized water. The rest of the experiment was the same as in Example 1. The experimental results are shown in Tables 1, 2, Figure 1 、 Figure 2 shown.

Claims

1. An application of a sinapinic acid plant growth regulator, characterized in that: The sinapinic acid plant growth regulator is used to alleviate the continuous cropping disorder of ramie plants. The raw materials include 10-30 parts of sinapinic acid and 1-3 parts of slow-release solvent by mass. The pH value of the sinapinic acid plant growth regulator is 5.0-7.0, and the concentration of the sinapinic acid is 200 mg·L -1 ~300mg·L -1 ; The slow-release solvent is ethanol with a volume concentration of 50~60%.

2. The use according to claim 1, characterized in that The soil used for the continuous cropping barrier is sandy clay loam where ramie crops have been planted continuously for 3-10 years.

3. The use according to claim 1, characterized in that The physical and chemical properties of the soil used for the continuous cropping barrier include: pH 4.0-7.0; soil conductivity 80-200 S / cm; organic matter content 20-200 g / kg; hydrolyzable nitrogen content 100-200 mg / kg; available phosphorus content 20-200 mg / kg; and available potassium content 20-300 mg / kg.

4. The use according to claim 1, wherein The sinapinic acid plant growth regulator is used to alleviate the continuous cropping disorder of ramie plants, and specifically comprises the following steps: (1) Cultivate ramie seedlings. After the ramie seedlings have grown, use sinapinic acid plant growth regulator to irrigate the roots of the ramie seedlings, 50-100 mL each time, once every three days; (2) After 30 days of root irrigation, the cultivation was completed and the plant height, stem diameter, SPAD value, total chlorophyll content, antioxidant enzyme activity and leaf transcriptome sequencing analysis were performed.

5. The use according to claim 4, characterized in that During the root irrigation treatment, the height of the ramie plant is 1.5-2 cm; and the irrigation range of the root irrigation treatment is within a radius of 2 cm from the ramie root.

6. The use according to claim 4, characterized in that During the root irrigation treatment, topdressing and pest control are performed. The fertilizer used in the topdressing includes Hogland nutrient solution, and the objects of the pest control treatment include armyworms and / or board armyworms.

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