A lysozyme effervescent tablet for surface disinfection of objects and preparation method thereof

Abstract

The invention relates to the technical field of disinfection products, and provides a lysozyme effervescent tablet for surface disinfection of an object and a preparation method thereof, wherein the components of the lysozyme effervescent tablet include lysozyme, an alkaline agent, an acidic agent, sodium lauryl sarcosinate, a disintegrant, carboxymethylated methoxy pectin and polyethylene glycol 6000. The combined use of carboxymethylated methoxy pectin and polyethylene glycol 6000 in the effervescent tablet of the invention can effectively improve the activity and stability of the lysozyme effervescent tablet during storage, and ensure that the lysozyme effervescent tablet can maintain a good disinfection effect when in use.

Description

Technical Field

[0001] The invention relates to the technical field of disinfection products, and in particular to a lysozyme effervescent tablet for disinfecting the surface of an object and a preparation method thereof. Background Art

[0002] With the frequent occurrence of public health events and people's attention to health, the demand for disinfection products has increased significantly. As a new type of disinfection product, disinfection effervescent tablets have attracted widespread attention due to their convenience and high efficiency. Effervescent tablets can quickly dissolve and release active ingredients after contact with water, and can quickly prepare disinfection solutions, which are suitable for disinfection needs in various occasions. Compared with traditional liquid disinfectants, effervescent tablets have the advantages of convenient storage, safe transportation, and simple use. In addition, the solid form of effervescent tablets makes them more stable during storage and reduces the risk of degradation of active ingredients. However, existing disinfection effervescent tablets are mostly based on chemical disinfectants, which may pose potential hazards to the environment and human body. Therefore, the development of a safe, environmentally friendly and efficient disinfection effervescent tablet has become the focus of current research.

[0003] Lysozyme is a naturally occurring antibacterial enzyme with broad-spectrum antibacterial activity, especially against Gram-positive bacteria. It hydrolyzes the peptidoglycan in the bacterial cell wall and destroys the structure of the bacteria, thereby achieving a bactericidal effect. Since lysozyme is naturally derived and has no toxic side effects, it has been widely used in the fields of food preservation and medical device disinfection in recent years. Since the activity of lysozyme is greatly affected by the environment, especially in a high temperature or humid environment, the activity will decrease, thereby affecting the disinfection effect. For this reason, it is necessary to study a highly stable lysozyme effervescent tablet for disinfection. Summary of the invention

[0004] In view of this, the present invention provides a lysozyme effervescent tablet with high stability for disinfecting the surface of an object.

[0005] The technical solution of the present invention is implemented as follows: On the one hand, the present invention provides a lysozyme effervescent tablet for surface disinfection of objects, comprising lysozyme, an alkaline agent, an acidic agent, sodium lauryl sarcosinate, a disintegrant, carboxymethylated methoxy pectin and polyethylene glycol 6000.

[0006] Specifically, carboxymethylated methoxy pectin forms hydrogen bonds and electrostatic interactions with lysozyme molecules through its carboxymethyl groups, which can effectively encapsulate and protect lysozyme and prevent it from being inactivated by environmental factors during storage. In addition, carboxymethylation also increases the hydrophilicity of pectin, making it more soluble in water, which helps to quickly release lysozyme when the effervescent tablet dissolves and improves the disinfection effect. Polyethylene glycol 6000 can stabilize the protein structure by forming a protective hydration layer to prevent lysozyme from denaturing or aggregating during storage.

[0007] The combination of carboxymethylated methoxy pectin and polyethylene glycol 6000 can form a more effective protective barrier to reduce the impact of the external environment on lysozyme. Pectin provides chemical protection, while polyethylene glycol 6000 provides physical stability. When the effervescent tablet is dissolved, the good solubility of carboxymethylated methoxy pectin and the stabilizing effect of polyethylene glycol 6000 can promote the rapid release and uniform distribution of lysozyme, thereby improving its disinfection effect.

[0008] On the basis of the above technical scheme, preferably, the effervescent tablet comprises the following components, calculated by weight: 1-3 parts of lysozyme, 10-30 parts of alkaline agent, 5-25 parts of acidic agent, 5-10 parts of sodium lauryl sarcosinate, 5-15 parts of disintegrant, 3-5 parts of carboxymethylated methoxy pectin and 5-8 parts of polyethylene glycol 6000.

[0009] On the basis of the above technical scheme, preferably, the preparation method of the carboxymethylated methoxy pectin is:

[0010] S1, dissolving methoxy pectin in water to obtain a pectin solution, then adding a sodium hydroxide solution, stirring evenly, and adjusting the pH of the solution to 10-12;

[0011] S2, slowly add sodium chloroacetate to the alkalized pectin solution under stirring, continue stirring and heat to 50°C, and react for 2-4 hours;

[0012] S3, after the reaction is completed, dilute hydrochloric acid is added to neutralize until the pH drops to 7, and carboxymethyl methoxy pectin is precipitated;

[0013] S4, filtering, washing the filter residue with deionized water for multiple times, and then drying to obtain carboxymethyl methoxy pectin.

[0014] On the basis of the above technical solution, preferably, the amount of sodium chloroacetate used is 1.5-2 times the mass of methoxy pectin.

[0015] On the basis of the above technical solution, preferably, the sodium hydroxide solution is a 5%-10% mass fraction sodium hydroxide aqueous solution, and the dilute hydrochloric acid is a 1-2 mol / L hydrochloric acid solution.

[0016] On the basis of the above technical scheme, preferably, the acidic agent is one or more combinations of tartaric acid, L-cysteine ​​and citric acid, the alkaline agent is one or more combinations of sodium bicarbonate, sodium carbonate and potassium bicarbonate, and the disintegrant is microcrystalline cellulose or cross-linked polyvinyl pyrrolidone.

[0017] On the basis of the above technical solution, preferably, polyethyleneimine is also included.

[0018] Specifically, polyethyleneimine (PEI) is a polymer with a large amount of positive charges. These positive charges can form ionic bonds or electrostatic interactions with negatively charged components in effervescent tablets (such as carboxymethylated methoxy pectin and lysozyme). This charge barrier can regulate and wrap the lysozyme molecules, making it difficult to release them quickly into the solution, thereby achieving the technical effect of slow release of lysozyme and extending the sterilization time. When lysozyme is slowly released, PEI can form a positively charged film on the surface of the object. This film can interact with the negatively charged bacterial outer membrane, increase the permeability of the bacterial cell membrane, and improve the cumulative sterilization effect of lysozyme.

[0019] Through these combined mechanisms, the addition of PEI effectively achieves the goal of sustained-release lysozyme, providing a long-lasting disinfection effect, allowing the effervescent tablet to prolong the effective disinfection time on the surface of objects after dissolution.

[0020] On the basis of the above technical solution, preferably, the mass ratio of polyethyleneimine to lysozyme is 2-5:1-3.

[0021] On the other hand, the present invention also provides a method for preparing a lysozyme effervescent tablet for surface disinfection of an object, comprising the following steps:

[0022] S1, pass the powdered raw material through a 80-100 mesh sieve for later use;

[0023] S2, dissolving carboxymethylated methoxy pectin and polyethylene glycol 6000 in deionized water, then adding lysozyme and stirring evenly, and then vacuum drying to obtain a lysozyme-pectin-PEG complex, and passing through an 80-100 mesh sieve for later use;

[0024] S3, mixing the lysozyme-pectin-PEG complex with an alkaline agent, an acidic agent, sodium lauryl sarcosinate, a disintegrant and polyethyleneimine in a mixer for 10-15 minutes, then adding 3%-5% of anhydrous ethanol by weight of the mixture, continuing to mix for 5-10 minutes and then passing through a 20-40 mesh sieve for wet granulation;

[0025] S4, after the wet granules in step S3 are dried, a lubricant is added and mixed evenly, and tableting is performed.

[0026] On the basis of the above technical solution, preferably, the lubricant is magnesium stearate or sodium lauryl sulfate, and the added amount is 5%-10% of the weight of the wet granules.

[0027] The lysozyme effervescent tablet for surface disinfection of an object and the preparation method thereof of the present invention have the following beneficial effects compared with the prior art:

[0028] (1) The combined use of carboxymethylated methoxy pectin and polyethylene glycol 6000 can effectively improve the activity and stability of lysozyme effervescent tablets during storage, ensuring that they have a good disinfection effect when used.

[0029] (2) The addition of PEI effectively achieved the goal of sustained-release lysozyme, providing a long-lasting disinfection effect, so that the effervescent tablet can prolong the effective disinfection time on the surface of objects after dissolution. DETAILED DESCRIPTION

[0030] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1

[0032] The lysozyme effervescent tablet of this embodiment comprises 25 g of lysozyme, 180 g of sodium bicarbonate, 150 g of tartaric acid, 75 g of sodium lauryl sarcosinate, 125 g of microcrystalline cellulose, 45 g of carboxymethylated methoxy pectin and 65 g of polyethylene glycol 6000.

[0033] Wherein, the preparation method of carboxymethylated methoxy pectin comprises the following steps:

[0034] S1, dissolving 20 g of methoxy pectin in 10 times the mass (200 g) of water to obtain a pectin solution, then adding 5% by mass aqueous sodium hydroxide solution, stirring evenly, and adjusting the pH of the solution to 10.

[0035] S2. While stirring, slowly add 36 g of sodium chloroacetate into the alkalized pectin solution, continue stirring and heat to 50° C., and react for 3 h.

[0036] S3, after the reaction is completed, 1 mol / L hydrochloric acid solution is added to neutralize until the pH drops to 7, and carboxymethyl methoxy pectin is precipitated.

[0037] S4, filtering, washing the filter residue with deionized water for multiple times, and then drying to obtain carboxymethyl methoxy pectin.

[0038] The preparation method of lysozyme effervescent tablets comprises the following steps:

[0039] S1, pass the powdered raw material through a 100-mesh sieve for later use.

[0040] S2, dissolving 45 g of carboxymethylated methoxy pectin and 65 g of polyethylene glycol 6000 in 1000 g of deionized water, then adding 25 g of lysozyme and stirring evenly, and then vacuum drying to obtain a lysozyme-pectin-PEG complex, and passing through a 100-mesh sieve for later use.

[0041] S3, the lysozyme-pectin-PEG complex prepared in step S2 is mixed with 180 g of sodium bicarbonate, 150 g of tartaric acid, 75 g of sodium lauryl sarcosinate and 125 g of microcrystalline cellulose in a mixer for 15 min, and then 4% of the mass of the mixture is added with anhydrous ethanol, and the mixture is mixed for 5 more minutes before wet granulation through a 20-mesh sieve.

[0042] S4, after the wet granules in step S3 are dried, 8% of the weight of the wet granules of magnesium stearate are added and mixed evenly, and then tabletted. The size of the effervescent tablet is 2.6 cm×2.6 cm×1 cm.

[0043] Example 2

[0044] The difference between Example 2 and Example 1 is that 40 g of polyethyleneimine is added to the components, and the preparation method of carboxymethylated methoxy pectin and the remaining components and their contents are the same as those in Example 1.

[0045] The preparation method of lysozyme effervescent tablets comprises the following steps:

[0046] S1, pass the powdered raw material through a 100-mesh sieve for later use.

[0047] S2, dissolving 45 g of carboxymethylated methoxy pectin and 65 g of polyethylene glycol 6000 in 1000 g of deionized water, then adding 25 g of lysozyme and stirring evenly, and then vacuum drying to obtain a lysozyme-pectin-PEG complex, and passing through a 100-mesh sieve for later use.

[0048] S3, the lysozyme-pectin-PEG complex prepared in step S2 is mixed with 180 g of sodium bicarbonate, 150 g of tartaric acid, 75 g of sodium lauryl sarcosinate, 125 g of microcrystalline cellulose and 40 g of polyethyleneimine in a mixer for 15 min, and then 4% of anhydrous ethanol by weight of the mixture is added, and the mixture is mixed for 5 more minutes before wet granulation through a 20-mesh sieve.

[0049] S4, after the wet granules in step S3 are dried, 8% of the weight of the wet granules of magnesium stearate are added and mixed evenly, and then tabletted. The size of the effervescent tablet is 2.6 cm×2.6 cm×1 cm.

[0050] Example 3

[0051] The lysozyme effervescent tablet of the present embodiment comprises 10 g of lysozyme, 150 g of sodium carbonate, 50 g of L-cysteine, 60 g of sodium lauryl sarcosinate, 50 g of cross-linked polyvinyl pyrrolidone, 40 g of carboxymethylated methoxy pectin, 70 g of polyethylene glycol 6000 and 30 g of polyethyleneimine.

[0052] Wherein, the preparation method of carboxymethylated methoxy pectin comprises the following steps:

[0053] S1, dissolving 20 g of methoxy pectin in 10 times the mass (200 g) of water to obtain a pectin solution, then adding 8% by mass aqueous sodium hydroxide solution, stirring evenly, and adjusting the pH of the solution to 11.

[0054] S2. While stirring, slowly add 40 g of sodium chloroacetate into the alkalized pectin solution, continue stirring and heat to 50° C., and react for 2 h.

[0055] S3, after the reaction is completed, add 2 mol / L hydrochloric acid solution to neutralize until the pH drops to 7, and carboxymethyl methoxy pectin precipitates.

[0056] S4, filtering, washing the filter residue with deionized water for multiple times, and then drying to obtain carboxymethyl methoxy pectin.

[0057] The preparation method of lysozyme effervescent tablets comprises the following steps:

[0058] S1, pass the powdered raw material through an 80-mesh sieve for later use.

[0059] S2, dissolving 40 g of carboxymethylated methoxy pectin and 70 g of polyethylene glycol 6000 in 1000 g of deionized water, then adding 10 g of lysozyme and stirring evenly, and then vacuum drying to obtain a lysozyme-pectin-PEG complex, and passing through an 80-mesh sieve for later use.

[0060] S3, the lysozyme-pectin-PEG complex prepared in step S2 is mixed with 150 g of sodium carbonate, 50 g of L-cysteine, 60 g of sodium lauryl sarcosinate, 50 g of cross-linked polyvinyl pyrrolidone and 30 g of polyethyleneimine in a mixer for 10 min, and then 5% of the mass of the mixture is added with anhydrous ethanol, and the mixture is mixed for another 5 min and then passed through a 20-mesh sieve for wet granulation.

[0061] S4, after the wet granules in step S3 are dried, sodium lauryl sulfate (5% by weight of the wet granules) is added and mixed evenly, and then tableted. The size of the effervescent tablet is 2.6 cm×2.6 cm×1 cm.

[0062] Example 4

[0063] The lysozyme effervescent tablet of the present embodiment comprises 30 g of lysozyme, 280 g of potassium bicarbonate, 250 g of citric acid, 100 g of sodium lauryl sarcosinate, 150 g of microcrystalline cellulose, 50 g of carboxymethylated methoxy pectin, 80 g of polyethylene glycol 6000 and 45 g of polyethyleneimine.

[0064] Wherein, the preparation method of carboxymethylated methoxy pectin comprises the following steps:

[0065] S1, dissolving 20 g of methoxy pectin in 10 times the mass (200 g) of water to obtain a pectin solution, then adding 10% by mass aqueous sodium hydroxide solution, stirring evenly, and adjusting the pH of the solution to 12.

[0066] S2. While stirring, slowly add 30 g of sodium chloroacetate into the alkalized pectin solution, continue stirring and heat to 50° C., and react for 4 h.

[0067] S3, after the reaction is completed, 1.5 mol / L hydrochloric acid solution is added to neutralize until the pH drops to 7, and carboxymethyl methoxy pectin is precipitated.

[0068] S4, filtering, washing the filter residue with deionized water for multiple times, and then drying to obtain carboxymethyl methoxy pectin.

[0069] The preparation method of lysozyme effervescent tablets comprises the following steps:

[0070] S1, pass the powdered raw material through a 100-mesh sieve for later use;

[0071] S2, dissolving 50 g of carboxymethylated methoxy pectin and 80 g of polyethylene glycol 6000 in 1000 g of deionized water, then adding 30 g of lysozyme and stirring evenly, and then vacuum drying to obtain a lysozyme-pectin-PEG complex, and passing through a 100-mesh sieve for later use;

[0072] S3, mixing the lysozyme-pectin-PEG complex prepared in step S2 with 280 g potassium bicarbonate, 250 g citric acid, 100 g sodium lauryl sarcosinate, 150 g microcrystalline cellulose and 45 g polyethyleneimine in a mixer for 15 min, then adding 3% anhydrous ethanol by weight of the mixture, continuing to mix for 5 min, and then passing through a 20-mesh sieve for wet granulation;

[0073] S4, after the wet granules in step S3 are dried, magnesium stearate accounting for 10% of the weight of the wet granules is added and mixed evenly, and then tableted. The size of the effervescent tablet is 2.6 cm×2.6 cm×1 cm.

[0074] Example 5

[0075] The lysozyme effervescent tablet of the present embodiment comprises 20 g of lysozyme, 100 g of sodium bicarbonate, 100 g of tartaric acid, 100 g of citric acid, 50 g of sodium lauryl sarcosinate, 100 g of cross-linked polyvinyl pyrrolidone, 30 g of carboxymethylated methoxy pectin, 50 g of polyethylene glycol 6000 and 20 g of polyethyleneimine.

[0076] Wherein, the preparation method of carboxymethylated methoxy pectin comprises the following steps:

[0077] S1, dissolving 20 g of methoxy pectin in 10 times the mass (200 g) of water to obtain a pectin solution, then adding 5%-10% by mass of an aqueous sodium hydroxide solution, stirring evenly, and adjusting the pH of the solution to 11.

[0078] S2. While stirring, slowly add 32 g of sodium chloroacetate into the alkalized pectin solution, continue stirring and heat to 50°C, and react for 2.5 hours.

[0079] S3, after the reaction is completed, 1.5 mol / L hydrochloric acid solution is added to neutralize until the pH drops to 7, and carboxymethyl methoxy pectin is precipitated.

[0080] S4, filtering, washing the filter residue with deionized water for multiple times, and then drying to obtain carboxymethyl methoxy pectin.

[0081] The preparation method of lysozyme effervescent tablets comprises the following steps:

[0082] S1, pass the powdered raw material through a 100-mesh sieve for later use;

[0083] S2, dissolving 30 g of carboxymethylated methoxy pectin and 50 g of polyethylene glycol 6000 in deionized water, then adding 20 g of lysozyme and stirring evenly, and then vacuum drying to obtain a lysozyme-pectin-PEG complex, and passing through a 100-mesh sieve for later use;

[0084] S3, mixing the lysozyme-pectin-PEG complex prepared in step S2 with 100 g of sodium bicarbonate, 100 g of tartaric acid, 100 g of citric acid, 50 g of sodium lauryl sarcosinate, 100 g of cross-linked polyvinyl pyrrolidone and 20 g of polyethyleneimine in a mixer for 12 min, then adding 3.5% of the mass of the mixture of anhydrous ethanol, continuing to mix for 5 min, and then passing through a 20-mesh sieve for wet granulation;

[0085] S4, after the wet granules in step S3 are dried, sodium lauryl sulfate (9% by weight of the wet granules) is added and mixed evenly, and then tableted. The size of the effervescent tablet is 2.6 cm×2.6 cm×1 cm.

[0086] Example 6

[0087] The lysozyme effervescent tablet of the present embodiment comprises 15 g of lysozyme, 300 g of sodium carbonate, 50 g of L-cysteine, 50 g of citric acid, 85 g of sodium lauryl sarcosinate, 80 g of microcrystalline cellulose, 35 g of carboxymethylated methoxy pectin, 75 g of polyethylene glycol 6000 and 35 g of polyethyleneimine.

[0088] Wherein, the preparation method of carboxymethylated methoxy pectin comprises the following steps:

[0089] S1, dissolving 20 g of methoxy pectin in 10 times the mass (200 g) of water to obtain a pectin solution, then adding 10% by mass aqueous sodium hydroxide solution, stirring evenly, and adjusting the pH of the solution to 12.

[0090] S2. While stirring, slowly add 40 g of sodium chloroacetate into the alkalized pectin solution, continue stirring and heat to 50° C., and react for 4 h.

[0091] S3, after the reaction is completed, add 2 mol / L hydrochloric acid solution to neutralize until the pH drops to 7, and carboxymethyl methoxy pectin precipitates.

[0092] S4, filtering, washing the filter residue with deionized water for multiple times, and then drying to obtain carboxymethyl methoxy pectin.

[0093] The preparation method of lysozyme effervescent tablets comprises the following steps:

[0094] S1, pass the powdered raw material through a 100-mesh sieve for later use;

[0095] S2, dissolving 35 g of carboxymethylated methoxy pectin and 75 g of polyethylene glycol 6000 in deionized water, then adding 15 g of lysozyme and stirring evenly, and then vacuum drying to obtain a lysozyme-pectin-PEG complex, and passing through a 100-mesh sieve for later use;

[0096] S3, mixing the lysozyme-pectin-PEG complex prepared in step S2 with 300 g of sodium carbonate, 50 g of L-cysteine, 50 g of citric acid, 85 g of sodium lauryl sarcosinate, 80 g of microcrystalline cellulose and 35 g of polyethyleneimine in a mixer for 10 min, then adding 5% of the mass of the mixture with anhydrous ethanol, continuing to mix for 5 min and then passing through a 20-mesh sieve for wet granulation;

[0097] S4, after the wet granules in step S3 are dried, magnesium stearate accounting for 10% of the weight of the wet granules is added and mixed evenly, and then tableted. The size of the effervescent tablet is 2.6 cm×2.6 cm×1 cm.

[0098] Comparative Example 1

[0099] The difference between Comparative Example 1 and Example 1 is that the carboxymethylated methoxy pectin is missing, and the rest of the contents are the same as Example 1.

[0100] Comparative Example 2

[0101] The difference between Comparative Example 2 and Example 1 is that polyethylene glycol 6000 is missing, and the rest of the contents are the same as Example 1.

[0102] Comparative Example 3

[0103] The difference between Comparative Example 3 and Example 1 is that carboxymethylated methoxy pectin and polyethylene glycol 6000 are missing, and the rest of the contents are the same as Example 1.

[0104] Comparative Example 4

[0105] The difference between Comparative Example 4 and Example 1 is that methoxy pectin is used to replace carboxymethylated methoxy pectin, and the rest of the contents are the same as Example 1.

[0106] The stability, disintegration time and bactericidal effect of the lysozyme effervescent tablets prepared in the examples and comparative examples were tested, and the results are shown in the table below.

[0107] The disintegration time is tested according to the dissolution method and requirements for effervescent tablets in the 2015 edition of the Chinese Pharmacopoeia.

[0108] The bactericidal effect detection method refers to the method specified in Appendix C4 "Test method for antibacterial performance of dissolution antibacterial (inhibitory) products" in GB / T15979-2002 "Hygiene Standard for Disposable Sanitary Products", where the test bacteria are Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 25922), and Candida albicans (ATCC 10231), the detection concentration is 1:100, and the action time is 30 minutes.

[0109]

[0110] Table 1 shows that the lysozyme effervescent tablets prepared by the embodiment of the present invention, its disintegration time is all shorter than the comparative example, and the bactericidal effect is all higher than the comparative example. It is known from Example 1 and Example 2 that although the disintegration time is slightly prolonged by adding polyethyleneimine, the bactericidal effect is significantly improved. It is known from Example 1 and Comparative Examples 1 to 3 that carboxymethylated methoxy pectin and polyethylene glycol 6000 lack any one, which will affect the disintegration time and bactericidal effect, and it is proved that carboxymethylated methoxy pectin and polyethylene glycol 6000 are composite, which can not only increase the bactericidal effect of lysozyme effervescent tablets, but also can shorten the disintegration time. It is known from Comparative Example 4 that after lacking carboxymethyl, the disintegration time is prolonged, and the bactericidal effect is also decreased simultaneously, and it is proved that carboxymethylated methoxy pectin has the effect of increasing pectin solubility and improving disinfection effect.

[0111] The long-term bactericidal effect of 30min-96h was tested, taking Escherichia coli as an example. The test results are shown in the table below.

[0112]

[0113] As shown in Table 2, the lysozyme effervescent tablets prepared in the embodiments of the present invention have a long-term bactericidal effect that is better than that of the comparative examples, wherein after adding polyethyleneimine (Example 2), the 96h bactericidal effect is still maintained at more than 95%. As shown in Example 1 and Comparative Examples 1 to 3, the carboxymethylated methoxy pectin and polyethylene glycol 6000 lack any one of them, and their bactericidal effect shows a significant downward trend over time, and the bactericidal effect after 96h is only 29% to 35%, which proves that carboxymethylated methoxy pectin and polyethylene glycol 6000 also have the purpose of extending the bactericidal effect. As shown in Comparative Example 4, methoxy pectin does not add carboxymethyl, and the protective effect on lysozyme is weakened, thereby reducing the bactericidal effect of the effervescent tablet.

[0114] Stability: Under the conditions of temperature 45±2℃ and humidity 60±5%, the disintegration time and sterilization effect are tested after storage for 12 months.

[0115]

[0116] As shown in Table 3, under high temperature and high humidity conditions, the lysozyme effervescent tablet of the present invention can still maintain a high bactericidal effect and a short disintegration time, which proves that carboxymethylated methoxy pectin and polyethylene glycol 6000 can improve the technical effect of lysozyme stability. The disintegration time of Comparative Example 4 is prolonged and the bactericidal effect is weakened, which shows that the carboxylmethyl group of carboxymethylated methoxy pectin interacts with the lysozyme molecule, can effectively wrap and protect the lysozyme, and thus improves the storage stability of the lysozyme effervescent tablet.

[0117] When the lysozyme effervescent tablet of the present invention is used for surface disinfection of an object, it is only necessary to put the lysozyme effervescent tablet into water (50 mL of water for 1 gram of lysozyme effervescent tablet), spray or soak it for disinfection after it disintegrates. It can also be used for disinfection of dentures, braces, orthodontic retainers, take one lysozyme effervescent tablet, add 40°C warm water, put dentures, braces, orthodontic retainers, etc. into it after it disintegrates, and completely submerge it in the water, soak it for 15-30 minutes, and if there are stains, scrub it with a soft-bristled toothbrush, and rinse it with clean water after cleaning.

[0118] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A lysozyme effervescent tablet for surface disinfection of objects, characterized in that: Calculated by weight, the effervescent tablet comprises the following components: 1-3 parts of lysozyme, 10-30 parts of alkaline agent, 5-25 parts of acidic agent, 5-10 parts of sodium lauryl sarcosinate, 5-15 parts of disintegrant, 3-5 parts of carboxymethylated methoxy pectin and 5-8 parts of polyethylene glycol 6000; The preparation method of the carboxymethylated methoxy pectin is: S1, dissolving methoxy pectin in water to obtain a pectin solution, then adding a sodium hydroxide solution, stirring evenly, and adjusting the pH of the solution to 10-12; S2, slowly add sodium chloroacetate to the alkalized pectin solution under stirring, continue stirring and heat to 50°C, and react for 2-4 hours; S3, after the reaction is completed, dilute hydrochloric acid is added to neutralize until the pH drops to 7, and carboxymethyl methoxy pectin is precipitated; S4, filtering, washing the filter residue with deionized water for multiple times, and then drying to obtain carboxymethyl methoxy pectin; The amount of sodium chloroacetate used is 1.5-2 times the mass of methoxy pectin.

2. A lysozyme effervescent tablet for surface disinfection according to claim 1, characterized in that: The sodium hydroxide solution is a sodium hydroxide aqueous solution with a mass fraction of 5%-10%, and the dilute hydrochloric acid is a hydrochloric acid solution with a mass fraction of 1-2 mol / L.

3. A lysozyme effervescent tablet for surface disinfection according to claim 1, characterized in that: The acidic agent is one or more combinations of tartaric acid, L-cysteine ​​and citric acid, the alkaline agent is one or more combinations of sodium bicarbonate, sodium carbonate and potassium bicarbonate, and the disintegrant is microcrystalline cellulose or cross-linked polyvinyl pyrrolidone.

4. A lysozyme effervescent tablet for surface disinfection according to claim 1, characterized in that: Also included are polyethyleneimines.

5. A lysozyme effervescent tablet for surface disinfection according to claim 4, characterized in that: The mass ratio of the polyethyleneimine to the lysozyme is 2-5:1-3.

6. The method for preparing the lysozyme effervescent tablet for surface disinfection of an object according to any one of claims 4 to 5, characterized in that: The following steps are involved: S1, pass the powdered raw material through a 80-100 mesh sieve for later use; S2, dissolving carboxymethylated methoxy pectin and polyethylene glycol 6000 in deionized water, then adding lysozyme and stirring evenly, and then vacuum drying to obtain a lysozyme-pectin-PEG complex, and passing through an 80-100 mesh sieve for later use; S3, mixing the lysozyme-pectin-PEG complex with an alkaline agent, an acidic agent, sodium lauryl sarcosinate, a disintegrant and polyethyleneimine in a mixer for 10-15 minutes, then adding 3%-5% of anhydrous ethanol by weight of the mixture, continuing to mix for 5-10 minutes and then passing through a 20-40 mesh sieve for wet granulation; S4, after the wet granules in step S3 are dried, a lubricant is added and mixed evenly, and tableting is performed.

7. The method for preparing the lysozyme effervescent tablet for surface disinfection of an object according to claim 6, characterized in that: The lubricant is magnesium stearate or sodium lauryl sulfate, and the added amount is 5%-10% of the weight of the wet granules.

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