A complete pet canned food for maintaining bone health and a method of preparation

Abstract

The application provides a full-value pet can for maintaining bone health and a preparation method, which comprises the following raw materials in mass parts: chicken breast meat 15-20 parts, chicken skeleton 3-7 parts, chicken heart 2-6 parts, beef 1-5 parts, beef liver 2-5 parts, tuna 5-10 parts, starch 9-14 parts, vegetables 17-23 parts, EPA 3-7 parts, vitamin K1-3 parts, folic acid 1-2 parts, nicotinic acid 0.5-1 part, mineral 2-6 parts, plant fermentation extract 5-9 parts, plant enzymolysis extract 3-6 parts and water 50-60 parts. The full-value pet can can provide full-value nutrition for pet cats, is beneficial to maintaining the bone health of the pet cats, prolongs the can storage time, inhibits the breeding of bacteria on the bottle opening and in the bottle after the can is opened, and improves the edible safety and storage time after the can is opened.

Description

Technical Field

[0001] This invention belongs to the field of pet food technology, specifically relating to a complete pet canned food for maintaining bone health and its preparation method. Background Technology

[0002] Canned pet food has many advantages, including high moisture content, ease of feeding, good palatability, and the ability to be mixed with dry food, making it a common wet pet food product. Canned pet food provides pets with the necessary nutrients, including protein, fat, vitamins, and minerals. Currently, canned pet food with natural ingredients is becoming increasingly popular. These canned foods typically do not contain artificial colors, flavors, preservatives, or fillers, but instead use real meat, vegetables, fruits, and other naturally derived ingredients, which are closer to the types of food pets might consume in the wild and are considered more beneficial to their health.

[0003] To address common skeletal diseases in pets, commercially available pet food typically increases the content of calcium and vitamin D. However, excessive calcium supplementation can actually increase the burden on a pet's body. While commercially available canned food products are very high in nutrients, their nutritional balance is not ideal and cannot meet the precise nutritional needs of pets throughout their growth period to maintain bone health.

[0004] Therefore, there is an urgent need for a natural, complete pet food that helps maintain bone health. Summary of the Invention

[0005] The purpose of this invention is to provide a complete pet food for maintaining bone health and a method for its preparation.

[0006] To achieve the above objectives, the present invention provides the following technical solution:

[0007] A complete pet food for maintaining bone health comprises the following ingredients by weight: 15-20 parts chicken breast, 3-7 parts chicken carcass, 2-6 parts chicken heart, 1-5 parts beef, 2-5 parts beef liver, 5-10 parts tuna, 9-14 parts starch, 17-23 parts vegetables, 3-7 parts EPA, 1-3 parts vitamin K, 1-2 parts folic acid, 0.5-1 part niacin, 2-6 parts minerals, 5-9 parts plant fermentation extract, 3-6 parts plant enzymatic hydrolysis extract, and 50-60 parts water;

[0008] The preparation method of the plant fermentation extract:

[0009] (1) Mix water shield, bracken, wild chrysanthemum and bamboo leaves in a mass ratio of 10:(7-9):(3-6):(2-4), crush them, and pass them through a 100-300 mesh sieve to obtain mixed powder A;

[0010] (2) Mix powder A with sterile water at a mass ratio of 1:10-15, add Lactobacillus branii, and carry out anaerobic fermentation at a fermentation temperature of 30-35℃ for 10-12 hours. Sterilize, add Lactobacillus plantarum, and continue anaerobic fermentation at a fermentation temperature of 30-35℃ for 8-10 hours. Sterilize to obtain the fermentation product.

[0011] (4) The fermentation product is filtered to obtain the filtrate, which is then concentrated and dried to obtain the plant fermentation extract.

[0012] Furthermore, the mass of Lactobacillus branii accounts for 2-4% of the total mass of mixed powder A and sterile water.

[0013] Furthermore, the mass of *Lactobacillus plantarum* accounts for 1-3% of the total mass of mixed powder A and sterile water.

[0014] Maintaining the stability of nutritional components is a critical issue in pet food, especially canned food. This invention adds EPA (eicosapentaenoic acid), vitamin K, folic acid, and niacin to the formula to support pets' bone and joint health. However, these components themselves have poor stability. EPA, an omega-3 fatty acid, is easily oxidized, and vitamin K, niacin, and folic acid may also degrade due to the effects of light, oxygen, temperature, and humidity, resulting in poor long-term feeding effects after opening. This invention adds plant fermentation extracts to the canned food ingredients to improve the stability of the bone health complex and enhance the feeding effect of canned food. Fermentation extracts from water shield, bracken, wild chrysanthemum, and bamboo leaves offer an innovative method to improve the stability of the bone health complex. The fermented extracts contain antioxidants that can slow down the oxidation and degradation of EPA, vitamin K, folic acid, and niacin. Simultaneously, the metabolites produced during fermentation may help improve the absorption and utilization efficiency of bone health nutrients.

[0015] Furthermore, the preparation method of the plant enzymatic hydrolysis extract is as follows:

[0016] (1) Mix bamboo fungus, schisandra chinensis, water chestnut peel and licorice in a mass ratio of 10:(1-3):(4-7):(6-8), pulverize and pass through a 100-300 mesh sieve to obtain mixed powder B;

[0017] (2) Mix powder B with sterile water at a mass ratio of 1:10-15, add cellulase, hydrolyze at 34-38℃ for 8-10 hours, inactivate the enzyme, add neutral protease, hydrolyze at 35-40℃ for 12-15 hours, inactivate the enzyme, and obtain the hydrolysis product.

[0018] (4) The enzymatic hydrolysis product is filtered to obtain the filtrate, which is then concentrated and dried to obtain the plant enzymatic hydrolysis extract.

[0019] Furthermore, the mass of cellulase accounts for 1-3% of the total mass of mixed powder B and sterile water.

[0020] Furthermore, the mass of neutral protease accounts for 3-5% of the total mass of mixed powder A and sterile water.

[0021] Because canned food is rich in nutrients, once opened, the contents come into contact with external microorganisms, which multiply rapidly, leading to visible or invisible bacterial contamination at the can lid or inside the can. This is especially detrimental to the health of young cats, who are very vulnerable. This invention, by incorporating enzymatic extracts of bamboo fungus, schisandra chinensis, water chestnut peel, and licorice, combined with antioxidant components from plant fermentation extracts, significantly improves the shelf life of canned food and inhibits bacterial growth at the can opening and inside the can after opening, thus enhancing the safety and shelf life of the canned food after opening.

[0022] Furthermore, the minerals comprise the following raw materials in parts by weight: 1-3 parts light calcium carbonate, 1-4 parts copper sulfate, 4-7 parts potassium iodide, 3-6 parts magnesium sulfate, 2-5 parts zinc sulfate, and 2-5 parts ferrous sulfate.

[0023] Furthermore, the vegetables comprise the following ingredients in parts by weight: 3-8 parts carrots, 1-4 parts pumpkin, 4-9 parts beets, 1-3 parts mung beans, and 2-7 parts broccoli.

[0024] Furthermore, the starch comprises the following raw materials in parts by weight: 1-3 parts corn starch, 2-6 parts potato starch, 2-5 parts sweet potato starch, 5-9 parts wheat starch, 1-3 parts cassava starch, and 3-7 parts kudzu starch.

[0025] This invention also provides a method for preparing complete pet food that maintains bone health, the method comprising the following steps:

[0026] (1) Thaw and process the chicken breast, chicken carcass, chicken heart, beef, beef liver, and tuna into a paste; dice the vegetables and mix them together;

[0027] (2) Mix starch and minerals in water until evenly mixed to obtain soup;

[0028] (3) Mix the minced meat and broth, and put them into an emulsifier for high-speed emulsification to obtain the material after the initial emulsification process;

[0029] (4) The material after the initial emulsification is cooked and matured to obtain the cooked and matured material, and the vegetable cubes are steamed.

[0030] (5) After cooling, the cooked and matured material is mixed with EPA, vitamin K, folic acid and niacin and then put into an emulsifier for high-speed emulsification; then it is mixed evenly with the cooled, steamed vegetables, plant fermentation extracts and plant enzymatic hydrolysate extracts.

[0031] (6) Canning and sterilization: Vacuum sealing machine is used to vacuum seal the cans, and sterilization is performed after sealing.

[0032] (7) Stasis, testing, and finished product processing.

[0033] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0034] 1. The complete pet food of the present invention can provide complete nutrition for pet cats, which is beneficial to maintaining the bone health of pet cats, improves the shelf life of canned food and inhibits the growth of bacteria at the bottle opening and inside the bottle after the can is opened, thereby improving the safety of the canned food after opening and its shelf life.

[0035] 2. This invention adds EPA, vitamin K, folic acid, and niacin to the formula, which can support the bone and joint health of pet cats. At the same time, adding plant fermentation extracts to the canned food ingredients can improve the stability of the bone health compound and improve the feeding effect of the canned food.

[0036] 3. This invention significantly improves the shelf life of canned food and inhibits bacterial growth at the bottle opening and inside the bottle after opening by adding enzymatic extracts of bamboo fungus, schisandra chinensis, water chestnut peel, and licorice, and combining them with antioxidant components in plant fermentation extracts. This improves the safety of canned food and its shelf life after opening. Detailed Implementation

[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] The bacteria and enzymes used in this invention are all commercially available products, as detailed below:

[0039] Lactobacillus fragrans, with accession number SHBCC D24371 JCM 2762, is deposited at the Shanghai Center for Microbiological Collection.

[0040] Lactobacillus plantarum, accession number: CCTCC CB 20081798, is deposited at the China Center for Type Culture Collection.

[0041] Lactobacillus helveticus, accession number CCTCC AB 2013168, is deposited at the China Center for Type Culture Collection.

[0042] Lactobacillus brevis, with accession number CCTCC DB 20081559, is deposited at the China Center for Type Culture Collection.

[0043] β-glucosidase, 50 U / mg, Jiangsu Aofu Biotechnology Co., Ltd.

[0044] Neutral protease, 50 U / mg, Hunan Yunbang Biotechnology Co., Ltd.

[0045] Cellulase, 50 U / mg, Hunan Yunbang Biotechnology Co., Ltd.

[0046] Pectinase, 50 U / mg, Hunan Yunbang Biotechnology Co., Ltd.

[0047] Example 1

[0048] This embodiment provides a complete pet food for maintaining bone health, comprising the following ingredients in parts by weight: 17 parts chicken breast, 5 parts chicken carcass, 4 parts chicken heart, 3 parts beef, 4 parts beef liver, 7 parts tuna, 11 parts starch, 20 parts vegetables, 5 parts EPA, 2 parts vitamin K, 1.5 parts folic acid, 0.7 parts niacin, 4 parts minerals, 7 parts plant fermentation extract, 4 parts plant enzymatic hydrolysis extract, and 55 parts water;

[0049] The preparation method of the plant fermentation extract:

[0050] (1) Mix water shield, bracken, wild chrysanthemum and bamboo leaves in a mass ratio of 10:8:5:3, crush them, and pass them through a 200-mesh sieve to obtain mixed powder A;

[0051] (2) Mix powder A at a mass ratio of 1:12 with sterile water, add Lactobacillus branii, and carry out anaerobic fermentation at a fermentation temperature of 32℃ for 11 hours. Sterilize, add Lactobacillus plantarum, and continue anaerobic fermentation at a fermentation temperature of 32℃ for 9 hours. Sterilize to obtain the fermentation product. The mass of Lactobacillus branii accounts for 3% of the total mass of powder A and sterile water, and the mass of Lactobacillus plantarum accounts for 2% of the total mass of powder A and sterile water.

[0052] (4) The fermentation product is filtered to obtain the filtrate, which is then concentrated and dried to obtain the plant fermentation extract.

[0053] The method for preparing the plant enzymatic hydrolysis extract is as follows:

[0054] (1) Mix bamboo fungus, schisandra chinensis, water chestnut peel and licorice in a mass ratio of 10:2:6:7, pulverize and pass through a 200-mesh sieve to obtain mixed powder B;

[0055] (2) Mix powder B with sterile water at a mass ratio of 1:13, add cellulase, hydrolyze at 36°C for 9 hours, inactivate the enzyme, add neutral protease, hydrolyze at 36°C for 14 hours, inactivate the enzyme, and obtain the hydrolysis product; the mass of cellulase accounts for 2% of the total mass of powder B and sterile water; the mass of neutral protease accounts for 4% of the total mass of powder A and sterile water.

[0056] (4) The enzymatic hydrolysis product is filtered to obtain the filtrate, which is then concentrated and dried to obtain the plant enzymatic hydrolysis extract.

[0057] The minerals comprise the following raw materials in parts by weight: 2 parts light calcium carbonate, 3 parts copper sulfate, 6 parts potassium iodide, 4 parts magnesium sulfate, 3 parts zinc sulfate, and 2 parts ferrous sulfate.

[0058] The vegetables comprise the following ingredients in parts by weight: 6 parts carrots, 3 parts pumpkin, 6 parts beets, 2 parts mung beans, and 5 parts broccoli.

[0059] The starch comprises the following raw materials in parts by weight: 2 parts corn starch, 5 parts potato starch, 3 parts sweet potato starch, 7 parts wheat starch, 2 parts cassava starch, and 5 parts kudzu starch.

[0060] The method for preparing complete pet food that maintains bone health includes the following steps:

[0061] (1) Thaw and process the chicken breast, chicken carcass, chicken heart, beef, beef liver, and tuna into a paste; dice the vegetables and mix them together;

[0062] (2) Mix starch and minerals in water until evenly mixed to obtain soup;

[0063] (3) Mix the minced meat and broth, and put them into an emulsifier for high-speed emulsification to obtain the material after the initial emulsification process;

[0064] (4) The material after the initial emulsification is cooked and matured to obtain the cooked and matured material, and the vegetable cubes are steamed.

[0065] (5) After cooling, the cooked and matured material is mixed with EPA, vitamin K, folic acid and niacin and then put into an emulsifier for high-speed emulsification; then it is mixed evenly with the cooled, steamed vegetables, plant fermentation extracts and plant enzymatic hydrolysate extracts.

[0066] (6) Canning and sterilization: Vacuum sealing machine is used to vacuum seal the cans, and sterilization is performed after sealing.

[0067] (7) Stasis, testing, and finished product processing.

[0068] Example 2

[0069] This embodiment provides a complete pet food for maintaining bone health, comprising the following ingredients in parts by weight: 15 parts chicken breast, 7 parts chicken carcass, 2 parts chicken heart, 5 parts beef, 2 parts beef liver, 10 parts tuna, 9 parts starch, 23 parts vegetables, 3 parts EPA, 3 parts vitamin K, 1 part folic acid, 1 part niacin, 2 parts minerals, 9 parts plant fermentation extract, 3 parts plant enzymatic hydrolysis extract, and 60 parts water.

[0070] The preparation method of the plant fermentation extract:

[0071] (1) Mix water shield, bracken, wild chrysanthemum and bamboo leaves in a mass ratio of 10:7:3:4, crush them, and pass them through a 100-mesh sieve to obtain mixed powder A;

[0072] (2) Mix powder A and sterile water at a mass ratio of 1:15, add Lactobacillus branii, and carry out anaerobic fermentation at a fermentation temperature of 30℃ for 12 hours. Sterilize, add Lactobacillus plantarum, and continue anaerobic fermentation at a fermentation temperature of 30℃ for 10 hours. Sterilize to obtain the fermentation product. The mass of Lactobacillus branii accounts for 2% of the total mass of powder A and sterile water, and the mass of Lactobacillus plantarum accounts for 3% of the total mass of powder A and sterile water.

[0073] (4) The fermentation product is filtered to obtain the filtrate, which is then concentrated and dried to obtain the plant fermentation extract.

[0074] The method for preparing the plant enzymatic hydrolysis extract is as follows:

[0075] (1) Mix bamboo fungus, schisandra chinensis, water chestnut peel and licorice in a mass ratio of 10:1:4:8, pulverize and pass through a 300-mesh sieve to obtain mixed powder B;

[0076] (2) Mix powder B with sterile water at a mass ratio of 1:10, add cellulase, hydrolyze at 38°C for 8 hours to inactivate the enzyme, add neutral protease, hydrolyze at 40°C for 12 hours to inactivate the enzyme, and obtain the hydrolysis product; the mass of cellulase accounts for 3% of the total mass of powder B and sterile water; the mass of neutral protease accounts for 3% of the total mass of powder A and sterile water.

[0077] (4) The enzymatic hydrolysis product is filtered to obtain the filtrate, which is then concentrated and dried to obtain the plant enzymatic hydrolysis extract.

[0078] The minerals comprise the following raw materials in parts by weight: 3 parts light calcium carbonate, 1 part copper sulfate, 7 parts potassium iodide, 3 parts magnesium sulfate, 5 parts zinc sulfate, and 2 parts ferrous sulfate.

[0079] The vegetables comprise the following ingredients in parts by weight: 8 parts carrots, 1 part pumpkin, 9 parts beets, 1 part mung beans, and 7 parts broccoli.

[0080] The starch comprises the following raw materials in parts by weight: 1 part corn starch, 6 parts potato starch, 2 parts sweet potato starch, 5 parts wheat starch, 1 part cassava starch, and 7 parts kudzu starch.

[0081] The method for preparing complete pet food that maintains bone health includes the following steps:

[0082] (1) Thaw and process the chicken breast, chicken carcass, chicken heart, beef, beef liver, and tuna into a paste; dice the vegetables and mix them together;

[0083] (2) Mix starch and minerals in water until evenly mixed to obtain soup;

[0084] (3) Mix the minced meat and broth, and put them into an emulsifier for high-speed emulsification to obtain the material after the initial emulsification process;

[0085] (4) The material after the initial emulsification is cooked and matured to obtain the cooked and matured material, and the vegetable cubes are steamed.

[0086] (5) After cooling, the cooked and matured material is mixed with EPA, vitamin K, folic acid and niacin and then put into an emulsifier for high-speed emulsification; then it is mixed evenly with the cooled, steamed vegetables, plant fermentation extracts and plant enzymatic hydrolysate extracts.

[0087] (6) Canning and sterilization: Vacuum sealing machine is used to vacuum seal the cans, and sterilization is performed after sealing.

[0088] (7) Stasis, testing, and finished product processing.

[0089] Comparative Example 1

[0090] The difference between this comparative example and Example 1 is that water shield, bracken, wild chrysanthemum, and bamboo leaves are mixed in a mass ratio of 1:1:1:1.

[0091] Comparative Example 2

[0092] The difference between this comparative example and Example 1 is that Lactobacillus branii was replaced with Lactobacillus helveticus; and Lactobacillus plantarum was replaced with Lactobacillus brevis.

[0093] Comparative Example 3

[0094] The difference between this comparative example and Example 1 is that: long-skirted bamboo fungus, southern schisandra, water chestnut peel, and licorice are mixed in a mass ratio of 1:1:1:1.

[0095] Comparative Example 4

[0096] The difference between this comparative example and Example 1 is that cellulase was replaced with β-glucosidase and neutral protease was replaced with pectinase.

[0097] Comparative Example 5

[0098] The difference between this comparative example and Example 1 is that 7 parts of plant fermentation extract were replaced with 3 parts; and 4 parts of plant enzymatic hydrolysis extract were replaced with 8 parts.

[0099] Comparative Example 6

[0100] The difference between this comparative example and Example 1 is that no plant fermentation extract was added; and 17 parts of chicken breast were replaced with 24 parts.

[0101] Comparative Example 7

[0102] The difference between this comparative example and Example 1 is that no plant enzymatic hydrolysate was added; and 17 parts of chicken breast were replaced with 21 parts.

[0103] Performance testing

[0104] 1. The Napoleon cat is a specific breed of cat characterized by short legs, possibly related to a genetic condition called "short-limb dwarfism." This condition can affect bone structure and bone density, although it doesn't mean all Napoleon cats will have low bone density or skeletal problems. In fact, short-limb dwarfism is usually caused by specific gene mutations that affect bone development, leading to shortened limbs.

[0105] Forty-four Napoleon cats of similar weight and age were randomly divided into 11 groups of four each. One group served as a normal control group, fed a normal diet. The remaining 10 groups underwent a 12-week osteoporosis model establishment process, and the successful modeling was confirmed by experimental bone mineral density measurement. The control group was fed a normal diet. Within the experimental groups, one group served as the model group, fed a normal diet, while the other nine groups were fed the pet canned food described in Examples 1-2 and Comparative Examples 1-7. After 12 weeks of feeding, the BMD values ​​of the Napoleon cats were measured using dual-energy X-ray absorptiometry (DXA). The results are shown in Table 1.

[0106] Table 1 Performance Test Results

[0107]

[0108]

[0109] The performance test results above show that Examples 1-2 have a better effect on bone density recovery in Napoleon cats, especially Example 1, which has the most outstanding overall performance. This is mainly due to the synergistic effect of multiple raw materials. The comparative example, however, did not employ the necessary technical solutions, resulting in significantly worse performance than the examples in the corresponding tests. These experimental results further demonstrate the importance of the technical solutions defined in this invention for its technical effectiveness.

[0110] 2. The antibacterial spectrum of the pet food prepared according to this invention was determined using the Oxford cup method. 100 μL of diluted bacterial suspension was evenly spread onto a solid agar plate. A sterile Oxford cup was gently placed on the culture medium and marked. 200 μL of complete pet food was then added to the Oxford cup. Deionized water was used as a blank control group. Each group had three replicates. The plates were then placed in a constant temperature incubator at 37°C and incubated for 12 hours. The diameter of the inhibition zone was then observed and measured. The size of the inhibition zone diameter was used to determine the inhibitory effect of the natural antibacterial extract on the test bacteria. The results are shown in Table 2.

[0111] Table 2. Inhibitory effect of complete pet food canned food on common foodborne pathogens.

[0112]

[0113] In Table 2, "+" indicates an inhibition zone diameter of 8-9 mm (inclusive); "++" indicates an inhibition zone diameter of 9-12 mm (inclusive); "+++" indicates an inhibition zone diameter greater than 12 mm; and "-" indicates no significant antibacterial effect. The results show that the complete pet food products of Examples 1-2 have a strong antibacterial effect against common foodborne pathogens, indicating that the combined use of each component yields the best results.

[0114] The above description represents the preferred embodiments of the present invention. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A complete pet food for maintaining bone health, characterized in that, The ingredients include the following parts by weight: 15-20 parts chicken breast, 3-7 parts chicken carcass, 2-6 parts chicken heart, 1-5 parts beef, 2-5 parts beef liver, 5-10 parts tuna, 9-14 parts starch, 17-23 parts vegetables, 3-7 parts EPA, 1-3 parts vitamin K, 1-2 parts folic acid, 0.5-1 part niacin, 2-6 parts minerals, 5-9 parts plant fermentation extract, 3-6 parts plant enzymatic hydrolysis extract, and 50-60 parts water; The preparation method of the plant fermentation extract: (1) Mix water shield, bracken, wild chrysanthemum and bamboo leaves in a mass ratio of 10:(7-9):(3-6):(2-4), crush them, and pass them through a 100-300 mesh sieve to obtain mixed powder A; (2) Mix powder A with sterile water at a mass ratio of 1:10-15, add Lactobacillus branii, and carry out anaerobic fermentation at a fermentation temperature of 30-35℃ for 10-12 hours. Sterilize, add Lactobacillus plantarum, and continue anaerobic fermentation at a fermentation temperature of 30-35℃ for 8-10 hours. Sterilize to obtain the fermentation product. (4) The fermentation product is filtered to obtain the filtrate, which is then concentrated and dried to obtain the plant fermentation extract; The method for preparing the plant enzymatic hydrolysis extract is as follows: (1) Mix bamboo fungus, schisandra chinensis, water chestnut peel and licorice in a mass ratio of 10:(1-3):(4-7):(6-8), pulverize and pass through a 100-300 mesh sieve to obtain mixed powder B; (2) Mix powder B with sterile water at a mass ratio of 1:10-15, add cellulase, hydrolyze at 34-38℃ for 8-10 hours, inactivate the enzyme, add neutral protease, hydrolyze at 35-40℃ for 12-15 hours, inactivate the enzyme, and obtain the hydrolysis product. (4) The enzymatic hydrolysis product is filtered to obtain the filtrate, which is then concentrated and dried to obtain the plant enzymatic hydrolysis extract.

2. The complete pet food for maintaining bone health according to claim 1, characterized in that, The mass of Lactobacillus branii accounts for 2-4% of the total mass of mixed powder A and sterile water.

3. The complete pet food for maintaining bone health according to claim 1, characterized in that, The mass of Lactobacillus plantarum accounts for 1-3% of the total mass of mixed powder A and sterile water.

4. The complete pet food for maintaining bone health according to claim 1, characterized in that, The mass of cellulase accounts for 1-3% of the total mass of mixed powder B and sterile water.

5. The complete pet food for maintaining bone health according to claim 1, characterized in that, The mass of neutral protease accounts for 3-5% of the total mass of mixed powder B and sterile water.

6. The complete pet food for maintaining bone health according to claim 1, characterized in that, The minerals mentioned include the following raw materials in parts by weight: 1-3 parts light calcium carbonate, 1-4 parts copper sulfate, 4-7 parts potassium iodide, 3-6 parts magnesium sulfate, 2-5 parts zinc sulfate, and 2-5 parts ferrous sulfate.

7. The complete pet food for maintaining bone health according to claim 1, characterized in that, The vegetables comprise the following ingredients in parts by weight: 3-8 parts carrots, 1-4 parts pumpkin, 4-9 parts beets, 1-3 parts mung beans, and 2-7 parts broccoli.

8. The complete pet food for maintaining bone health according to claim 1, characterized in that, The starch comprises the following raw materials in parts by weight: 1-3 parts corn starch, 2-6 parts potato starch, 2-5 parts sweet potato starch, 5-9 parts wheat starch, 1-3 parts cassava starch, and 3-7 parts kudzu starch.

9. A method for preparing a complete pet food for maintaining bone health as described in any one of claims 1-8, characterized in that, The preparation method includes the following steps: (1) Thaw and process the chicken breast, chicken carcass, chicken heart, beef, beef liver, and tuna into a paste; dice the vegetables and mix them together; (2) Mix starch and minerals in water until evenly mixed to obtain soup; (3) Mix the minced meat and broth, and put them into an emulsifier for high-speed emulsification to obtain the material after the initial emulsification process; (4) The material after the initial emulsification is cooked and matured to obtain the cooked and matured material, and the diced vegetables are steamed. (5) After cooling, the cooked and matured material is mixed with EPA, vitamin K, folic acid and niacin and then put into an emulsifier for high-speed emulsification; then it is mixed evenly with the cooled, steamed vegetables, plant fermentation extracts and plant enzymatic hydrolysate extracts. (6) Canning and sterilization: Vacuum sealing is performed using a vacuum sealing machine, followed by sterilization. (7) Stasis, testing, and finished product processing.

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