A cell culture device and a cell culture method thereof

Abstract

The present invention relates to the field of cell culture technology, and discloses a cell culture device and a culture method thereof, comprising a culture box and a culture rack, wherein the culture rack is located in the culture box, and the culture rack comprises a culture seat, a connecting piece and a controllable sealing piece, wherein the culture seats are arranged in a linear array in the culture box, and the connecting piece is arranged between adjacent culture seats. In the present invention, during the cell culture process, a partition, a culture room and a sliding door together form a partition, and the partition is sealed. During the opening period, the remaining cells in the culture box are avoided from being affected, thereby ensuring a stable and suitable growth environment for the entire cells in the culture box. The present invention has the characteristics of strong practicality and a stable growth environment.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a cell culture device and a culture method thereof. Background Art

[0002] Cell culture is a technology that simulates the growth environment of cells in vivo under in vitro conditions. This technology has a wide range of applications in biology, medicine, pharmacy, bioengineering and other fields, such as drug screening, disease model research, gene expression analysis, cell therapy, etc. The basic principle of cell culture is to provide cells with a suitable growth environment, including nutrients, temperature, pH value, oxygen and carbon dioxide concentrations, etc. Cells can proliferate and differentiate in such an environment, thereby conducting various biological studies.

[0003] Cell culture incubators are devices used to culture, preserve and test bacteria, molds, microorganisms, etc. They are widely used in food, biology and other technical fields. With the continuous deepening of cell research, the requirements for cell culture incubators are getting higher and higher. For example, in the process of cell culture, temperature and humidity have a great influence on the results of culture. If they are not well controlled, the efficiency of cell culture will be reduced or even fail.

[0004] When the existing cell culture device is used to culture cells inside the culture cabinet, the inside of the culture box will be connected with the outside, so that the temperature environment inside the culture box changes, which affects the cell culture inside the culture box. If it is not well controlled, it will lead to reduced efficiency or even failure of cell culture. For example, the invention patent with publication number CN118146944A discloses a constant temperature incubator for stem cell culture, wherein a carrying plate is provided inside the constant temperature incubator body, two guide rails are symmetrically arranged on the inner wall of the constant temperature incubator body, and guide grooves are provided inside the guide rails. Both sides of the carrying plate are located inside the guide grooves, and the culture box is placed on the carrying plate. However, during the use of the invention, in addition to the target culture medium, The rest of the culture medium is exposed to the external air, which may easily cause the cell culture efficiency to decrease or even fail. For example, the invention patent with publication number CN113528325A discloses an electric constant temperature incubator that is easy to observe, which relates to the technical field of electric constant temperature incubators. By setting the box body to a spherical shape and keeping it stable through a right-angle support frame, the heating module can use the existing technical means of the electric constant temperature incubator to heat the inside of the box body, and a curved observation window is vertically opened on the side wall of the spherical box body. During the use of this invention, the rest of the culture medium will be affected during the process of taking and placing the culture medium inside the box body. Therefore, it is very necessary to design a cell culture device and a culture method thereof with strong practicality and a stable growth environment. Summary of the invention

[0005] The object of the present invention is to provide a cell culture device and a culture method thereof to solve the problems raised in the above background technology.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a cell culture device, comprising an incubator and a culture rack, wherein the culture rack is located in the incubator, the culture rack comprises a culture seat, a connecting piece and a controllable sealing piece, the culture seats are arranged in a linear array in the incubator, the connecting piece is arranged between adjacent culture seats, the controllable sealing piece is arranged on the connecting piece, culture rooms are evenly opened on the peripheral wall of the culture seat, the side wall of the incubator is evenly distributed with access ports, the access ports and the culture seats are arranged in a one-to-one correspondence, a sliding door is movably installed in the access ports, partitions are evenly distributed in the incubator, the partitions and the access ports are arranged in a one-to-one correspondence, the partitions and the culture rooms are evenly distributed. and the sliding doors together form a partition, the partition includes a fixed frame, a telescopic frame, a telescopic cavity, a spring and a telescopic plate, the fixed frame is fixedly installed on the inner wall of the incubator, the telescopic frame is arranged at one end of the fixed frame, the telescopic cavity is opened in the fixed frame, one end of the telescopic frame extends into the telescopic cavity and the telescopic plate is fixedly installed, the spring is fixedly installed between the telescopic plate and the telescopic cavity, the spring is evenly distributed in the telescopic cavity, a reinforced sealing part is provided at the other end of the telescopic frame, a temperature control component is provided on the outer wall of the incubator, the temperature control component performs temperature control on the incubator and the partition, a driving part is provided at the lower end of the incubator, and the driving part drives the incubator rack to rotate.

[0007] According to the above technical solution, the connecting part includes a partition plate 1 and a connecting shaft, the partition plate 1 is fixedly installed on the incubator, the connecting shaft is movably installed in the middle position of the partition plate 1, the upper and lower ends of the connecting shaft 1 are respectively fixedly connected to the culture seat by fastening bolts, a movable groove is opened in the partition plate 1, and the controllable sealing part is arranged in the movable groove.

[0008] According to the above technical solution, the controllable seal includes a partition plate 2, an arc-shaped ventilation groove 1 and an arc-shaped ventilation groove 2, the partition plate 2 is movably installed in the movable groove through a control component, the arc-shaped ventilation groove 1 and the arc-shaped ventilation groove 2 are respectively opened on the partition plate 1 and the partition plate 2, the arc-shaped ventilation groove 1 and the arc-shaped ventilation groove 2 are respectively arranged in a linear array on the partition plate 1 and the partition plate 2, the arc-shaped ventilation groove 1 and the arc-shaped ventilation groove 2 are arranged one by one, and the arc-shaped ventilation groove 1 and the arc-shaped ventilation groove 2 are evenly distributed around the circumference.

[0009] According to the above technical solution, the control component includes a drive motor 1, a half-tooth gear, a reciprocating groove and a rack. The drive motor 1 is fixedly mounted on the partition plate 1. The reciprocating groove is opened at the upper end of the partition plate 2. The output end of the drive motor 1 extends into the reciprocating groove and is fixedly mounted with the half-tooth gear. The rack is symmetrically fixedly mounted on the side wall of the reciprocating groove. The half-tooth gear can and can only mesh with the rack at one end at the same time.

[0010] According to the above technical solution, the reinforced seal includes a sealing strip, a roller, a sealing groove and a groove. The sealing strip is fixedly installed at the other end of the telescopic frame, the roller is arranged at the four corners of the sealing strip, the sealing groove and the culture room are arranged in a one-to-one correspondence, the sealing strip and the sealing groove match each other, the roller is arranged at the four corners of the sealing strip, the groove is arranged at the four corners of the sealing groove, the roller is movably installed in the corresponding groove, and the left and right groove bodies of adjacent sealing grooves are arranged overlappingly.

[0011] According to the above technical solution, the temperature control assembly includes a temperature control seat, temperature control plate 1 and temperature control plate 2. The temperature control seat is fixedly installed on the outer wall of the incubator, the temperature control plate 1 is arranged on the inner wall of the incubator, and the temperature control plate 2 is arranged on the inner wall of the fixed frame. The temperature control seat is electrically connected to the temperature control plate 1 and the temperature control plate 2 respectively through wires.

[0012] According to the above technical solution, the driving member includes a second driving motor, a driving gear and a driven gear. The second driving motor is fixedly installed at the lower edge of the incubator. The output end of the second driving motor is fixedly installed with the driving gear. The lower end of the lowermost connecting shaft extends out of the incubator and is fixedly installed with a driven gear. The driving gear and the driven gear are meshed.

[0013] According to the above technical solution, the incubator includes a top cover, a box body, a bottom plate and support legs, the top cover, the box body, the bottom plate and the support legs are arranged in sequence from top to bottom, the top cover and the box body and the bottom plate are fixedly connected by fastening bolts respectively, the support legs are evenly arranged in a circle at the lower end of the bottom plate, the side wall of the box body is provided with a transparent observation window, and the transparent observation window is mirror-symmetrically arranged on both sides of the box body.

[0014] A cell culture method comprising the following steps:

[0015] S1, prepare culture medium: basal medium contains DMEM or RPMI1640, supplements contain fetal bovine serum and antibiotics;

[0016] S2, cell inoculation: inoculate the cell suspension into the culture flask to ensure that the cells are evenly distributed in the culture medium;

[0017] S3, setting culture conditions: setting the culture temperature inside the incubator through the temperature control component. In the present application, the interior of the incubator is configured as multiple layers of separate culture chambers through a partition plate 1, and different culture conditions can be set. Each layer of the culture chamber is correspondingly provided with a take-out port, and each layer of the separate culture chamber is provided with a temperature control plate 1 and a corresponding temperature sensor, and different culture temperatures can be set; at the same time, a connection setting can also be performed, and multiple layers of separate culture chambers are connected through the control component to form a culture chamber with unified culture conditions;

[0018] S4, placing in culture: open the sliding door, place the culture bottle in the culture room through the access port, and place one culture bottle in each culture room. After placing, close the sliding door, drive the culture rack to rotate through the control component, and culture the cells inside;

[0019] S5, regular liquid replacement: fresh culture medium is replaced every - days to maintain the growth state of cells. When taking and placing culture bottles, only one culture bottle is taken and placed each time. The partition, culture room and sliding door together form a partition, which is sealed to avoid affecting the remaining cells inside the incubator. After opening the sliding door, take out the culture medium inside, replace it, and successfully place it. The temperature inside the partition needs to be controlled and adjusted by the temperature control component, and because the range of the partition is small, the temperature control can be quickly adjusted to quickly adjust the re-placed culture bottle to a suitable growth environment temperature. When the internal temperature of the culture room to be opened is the same as the culture temperature inside the incubator, the control component can continue to rotate the culture rack to ensure a stable and suitable growth environment for the remaining cells inside. When the culture conditions of different layers of culture rooms are unified, each layer of separate culture rooms can also be temporarily isolated and protected by the control component when the culture bottles are taken and placed, which further plays a role of early warning and prevention, avoiding leakage of the partition and affecting the overall cells of the incubator.

[0020] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0021] (1) In the present application, the culture space inside the incubator is configured as multiple layers of separate culture chambers through a partition plate, and different culture conditions can be set. Each layer of the culture chamber is provided with a corresponding access port, and each layer of the separate culture chamber is provided with a temperature control component to adjust its temperature, and different culture temperatures can be set. At the same time, it can also be connected. The multiple layers of separate culture chambers are connected through the control component to form a culture chamber with unified culture conditions;

[0022] (2) The partition, the culture room and the sliding door together form a partition, which is sealed to avoid affecting the other cells inside the incubator during opening, thus ensuring a stable and suitable growth environment for the entire cell in the incubator;

[0023] (3) Each time the culture medium is taken out and placed, the temperature inside the compartment needs to be controlled and adjusted by the temperature control component. Since the compartment is small, the temperature control can be adjusted quickly, and the replaced culture bottle can be quickly adjusted to a suitable growth environment temperature, which is conducive to the cells quickly being in a suitable growth environment and proliferating and differentiating in a suitable growth environment;

[0024] (4) When the culture conditions of different layers of culture chambers are unified, each layer of individual culture chambers can also be temporarily isolated and protected through the control component when the culture bottles are taken out and placed, which further plays the role of early warning and prevention, avoids leakage in the partitions, and affects the overall cells of the incubator, which is conducive to reducing overall losses. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0026] Figure 1 It is a three-dimensional schematic diagram of the present invention;

[0027] Figure 2 is a first partial stereoscopic schematic diagram of the present invention;

[0028] Figure 3 is a second partial stereoscopic schematic diagram of the present invention;

[0029] Figure 4 is a third partial stereoscopic schematic diagram of the present invention;

[0030] Figure 5 is a fourth partial stereoscopic schematic diagram of the present invention;

[0031] Figure 6 is a fifth partial stereoscopic schematic diagram of the present invention;

[0032] Figure 7 is a sixth partial stereoscopic schematic diagram of the present invention;

[0033] Figure 8 is a top cross-sectional schematic diagram of the present invention;

[0034] Fig. 9 It is a three-dimensional schematic diagram of the partition of the present invention;

[0035] Fig.10 It is a partial three-dimensional schematic diagram of the partition of the present invention;

[0036] In the figure: 1-culture box, 11-top cover, 12-box, 13-bottom plate, 14-support leg, 2-culture rack, 21-culture seat, 211-culture room, 22-connector, 221-partition plate 1, 2211-movable groove, 222-connecting shaft, 23-controllable seal, 231-partition plate 2, 232-arc ventilation groove 1, 233-arc ventilation groove 2, 3-sliding door, 4-partition, 41-fixed frame, 42-telescopic frame, 43-telescopic Shrinkage cavity, 44-spring, 45-telescopic plate, 5-temperature control component, 51-temperature control seat, 52-temperature control plate one, 53-temperature control plate two, 6-driving member, 61-driving motor two, 62-driving gear, 63-driven gear, 7-control component, 71-driving motor one, 72-half-tooth gear, 73-reciprocating groove, 74-rack, 8-reinforced seal, 81-sealing strip, 82-roller, 83-sealing groove, 84-groove, 9-transparent observation window. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] See also Figure 1-10The present invention provides a technical solution: a cell culture device, comprising a culture box 1 and a culture rack 2, wherein the culture rack 2 is located in the culture box 1, and the culture rack 2 comprises a culture seat 21, a connecting piece 22 and a controllable sealing piece 23, wherein the culture seats 21 are arranged in a linear array in the culture box 1, the connecting piece 22 is arranged between adjacent culture seats 21, and the controllable sealing piece 23 is arranged on the connecting piece 22, and culture rooms 211 are evenly opened on the peripheral wall of the culture seats 21, and the side wall of the culture box 1 is evenly distributed with access ports, and the access ports and the culture seats 21 are arranged one-to-one, and a sliding door 3 is movably installed in the access ports, and partitions 4 are evenly distributed in the culture box 1, and the partitions 4 and the access ports are arranged one-to-one, and the partitions 4 and the culture rooms 211 and the sliding door 3 form a common shape. The partition 4 comprises a fixed frame 41, a telescopic frame 42, a telescopic cavity 43, a spring 44 and a telescopic plate 45. The fixed frame 41 is fixedly mounted on the inner wall of the incubator 1. The telescopic frame 42 is arranged at one end of the fixed frame 41. The telescopic cavity 43 is provided in the fixed frame 41. One end of the telescopic frame 42 extends into the telescopic cavity 43 and the telescopic plate 45 is fixedly mounted thereon. The spring 44 is fixedly mounted between the telescopic plate 45 and the telescopic cavity 43. The spring 44 is evenly distributed in the telescopic cavity 43. A reinforcing seal 8 is provided at the other end of the telescopic frame 42. A temperature control component 5 is provided on the outer wall of the incubator 1. The temperature control component 5 performs temperature control on the incubator 1 and the partition. A driving component 6 is provided at the lower end of the incubator 1. The driving component 6 drives the culture rack 2 to rotate.

[0039] When the present invention is used for cell culture, a culture space is created by the culture box 1, and the temperature of the culture box 1 is controlled and adjusted by the temperature control component 5 to create a suitable growth environment. The culture rack 2 is arranged in the culture box 1 and driven to rotate by the driving member 6. When the cells are cultured, the culture bottle is placed in the culture room 211, and only one culture bottle is placed in each culture room 211;

[0040] In the present application, the culture space inside the incubator 1 is configured as multiple layers of separate culture chambers through a partition plate 221, and different culture conditions can be set. Each layer of the culture chamber is correspondingly provided with a take-out port, and each layer of the separate culture chamber is provided with a temperature control component 5 to adjust its temperature, and different culture temperatures can be set; at the same time, a connection setting can also be performed, and multiple layers of separate culture chambers are connected through a control component 7 to form a culture chamber with uniform culture conditions;

[0041] Each time when taking and placing a culture bottle, only one culture bottle is taken and placed each time. The partition 4, the culture room 211 and the sliding door 3 together form a partition. The partition is sealed. During the opening period, it is avoided to affect the remaining cells in the incubator 1. The sliding door 3 can be provided with an electromagnetic lock for switch control. After opening the sliding door 3, the culture medium inside is taken out and replaced. After it is successfully placed, the temperature inside the partition needs to be controlled and adjusted by the temperature control component 5. Since the range of the partition is small, the temperature control can be adjusted quickly to quickly adjust the re-placed culture bottle to a suitable growth environment temperature. The temperature of the partition is monitored and adjusted by the temperature control component 5. When the internal temperature of the culture room 211 to be opened is the same as the internal culture temperature of the incubator 1, the culture rack 2 can be continuously rotated by the control component 7 to ensure a stable and suitable growth environment for the remaining cells inside.

[0042] When the culture chambers on different layers have the same culture conditions, when the culture bottles are taken out and placed, the control component 7 can also be used to temporarily isolate and protect the individual culture chambers on each layer, which further plays a role of early warning and prevention, avoids leakage between the partitions, and affects the overall cells of the incubator 1, which is conducive to reducing the overall loss;

[0043] The partition 4 includes a fixed frame 41, a telescopic frame 42, a telescopic cavity 43, a spring 44 and a telescopic plate 45. Through the design of the partition 4 and the reinforced seal 8, a closed partition can be formed between the culture room 211 and the sliding door 3. The culture bottles to be taken out and placed are separated by the partition, so as to ensure the overall growth environment inside the incubator 1. The growth environment is stable and avoids the influence of the external environment on it, which is conducive to the smooth progress of cell culture. At the same time, due to the small range of the partition, rapid temperature control can be performed to quickly adjust the re-placed culture bottles to a suitable growth environment temperature, which is conducive to the cells quickly being in a suitable growth environment and proliferating and differentiating in a suitable growth environment;

[0044] Specifically, the connecting member 22 includes a partition plate 221 and a connecting shaft 222, the partition plate 221 is fixedly installed on the culture box 1, the connecting shaft 222 is movably installed in the middle position of the partition plate 221, the upper and lower ends of the connecting shaft 222 are respectively fixedly connected to the culture seat 21 by fastening bolts, and a movable groove 2211 is opened in the partition plate 221, and the controllable sealing member 23 is arranged in the movable groove 2211;

[0045] Specifically, the controllable seal 23 includes a partition plate 231, an arc-shaped ventilation groove 1 232 and an arc-shaped ventilation groove 233. The partition plate 231 is movably installed in the movable groove 2211 through the control component 7. The arc-shaped ventilation groove 1 232 and the arc-shaped ventilation groove 2 233 are respectively arranged on the partition plate 1 221 and the partition plate 2 231. The arc-shaped ventilation groove 1 232 and the arc-shaped ventilation groove 2 233 are respectively arranged in a linear array on the partition plate 1 221 and the partition plate 2 231. The arc-shaped ventilation groove 1 232 and the arc-shaped ventilation groove 2 233 are arranged in a one-to-one correspondence, and the arc-shaped ventilation groove 1 232 and the arc-shaped ventilation groove 2 233 are evenly distributed around the circumference.

[0046] The controllable seal 23 includes a partition plate 231, an arc-shaped ventilation groove 1 232 and an arc-shaped ventilation groove 233. The partition plate 231 is arranged in the partition plate 1 221 and can be rotated back and forth in a certain angle by the control component 7. When rotating, the arc-shaped ventilation groove 233 is driven to rotate, thereby adjusting the interaction area between the arc-shaped ventilation groove 233 and the arc-shaped ventilation groove 1 232. When the arc-shaped ventilation groove 1 232 and the arc-shaped ventilation groove 233 are in contact with each other, different culture chambers are connected through and through, so that uniform culture conditions can be formed;

[0047] Specifically, the control assembly 7 includes a driving motor 1 71, a half-toothed gear 72, a reciprocating groove 73 and a rack 74. The driving motor 1 71 is fixedly mounted on the partition plate 1 221. The reciprocating groove 73 is provided at the upper end of the partition plate 231. The output end of the driving motor 1 71 extends into the reciprocating groove 73 and is fixedly mounted with the half-toothed gear 72. The rack 74 is symmetrically fixedly mounted on the side wall of the reciprocating groove 73. The half-toothed gear 72 can and can only mesh with the rack 74 at one end at the same time.

[0048] The driving motor 71, the half-toothed gear 72, the reciprocating groove 73 and the rack 74 are all common technical means in the technical field. In the case of not interfering with the rotation of the culture seat 21, the existing models can be selected according to the design requirements. No more details are given here. The driving motor 71 is provided with a control terminal through an electrical connection outside, and the control terminal is used to control the switch of the driving motor 71.

[0049] Specifically, the reinforced seal 8 includes a sealing strip 81, a roller 82, a sealing groove 83 and a groove 84, the sealing strip 81 is fixedly installed at the other end of the telescopic frame 42, the roller 82 is arranged at the four corners of the sealing strip 81, the sealing groove 83 and the culture room 211 are arranged in a one-to-one correspondence, the sealing strip 81 and the sealing groove 83 match, the roller 82 is arranged at the four corners of the sealing strip 81, the groove 84 is arranged at the four corners of the sealing groove 83, the roller 82 is movably installed in the corresponding groove 84, and the left and right grooves of the adjacent sealing grooves 83 are overlapped;

[0050] The sealing strip 81 matches the sealing groove 83, and the sealing strip 81 can be completely embedded in the sealing groove 83, which is helpful for the sealing of the partition. The setting of the roller 82 can facilitate the rotation of the culture seat 21, and avoid the culture seat 21 and the sealing strip 81 from generating large friction during the rotation process, so that both sides are damaged. The roller 82 can be completely embedded in the groove 84, which is helpful for the matching of the sealing strip 81 and the sealing groove 83, so that the sealing strip 81 and the sealing groove 83 are fully in contact, thereby ensuring the sealing of the partition.

[0051] Specifically, the temperature control assembly 5 includes a temperature control seat 51, a temperature control plate 1 52, and a temperature control plate 2 53. The temperature control seat 51 is fixedly mounted on the outer wall of the incubator 1, the temperature control plate 1 52 is arranged on the inner wall of the incubator 1, and the temperature control plate 2 53 is arranged on the inner wall of the fixed frame 41. The temperature control seat 51 is electrically connected to the temperature control plate 1 52 and the temperature control plate 2 53 through wires.

[0052] The temperature control assembly 5 includes a temperature control seat 51, a temperature control board 1 52 and a temperature control board 2 53. The temperature control seat 51 is the central control end. The inner wall of the incubator 1 is respectively provided with a temperature control board 1 52 and a corresponding temperature sensor to control and monitor the temperature in the incubator 1. Each culture chamber in the incubator 1 is provided with a separate temperature control board 1 52 and a corresponding temperature sensor. The inner wall of the fixed frame 41 is respectively provided with a temperature control board 2 53 and a corresponding temperature sensor to control and monitor the temperature in the compartment. Each compartment is provided with a separate temperature control board 2 53 and a corresponding temperature sensor.

[0053] Specifically, the driving member 6 includes a second driving motor 61, a driving gear 62 and a driven gear 63. The second driving motor 61 is fixedly installed at the lower edge of the incubator 1. The output end of the second driving motor 61 is fixedly installed with the driving gear 62. The lower end of the lowermost connecting shaft 222 extends out of the incubator 1 and is fixedly installed with the driven gear 63. The driving gear 62 is meshed with the driven gear 63.

[0054] The second driving motor 61, the driving gear 62 and the driven gear 63 are all common technical means in the technical field. Appropriate models can be selected according to the needs. No further details are given here. The second driving motor 61 is also provided with a control terminal outside, and the control terminal is used to control the switch of the motor.

[0055] Specifically, the incubator 1 includes a top cover 11, a box body 12, a bottom plate 13 and support legs 14, wherein the top cover 11, the box body 12, the bottom plate 13 and the support legs 14 are arranged in sequence from top to bottom, and the top cover 11, the box body 12 and the bottom plate 13 are fixedly connected by fastening bolts respectively, and the support legs 14 are evenly arranged at the lower end of the bottom plate 13 in a circumferential manner, and a transparent observation window 9 is provided on the side wall of the box body 12, and the transparent observation window 9 is arranged on both sides of the box body 12 in a mirror-symmetrical manner;

[0056] The incubator 1 includes a top cover 11, a box body 12, a bottom plate 13 and support legs 14, which are used for stable support. The number of support legs 14 is not limited. In the present application, three support legs 14 are provided and are evenly distributed around the circumference to form a triangular stable support. The transparent observation window 9 is provided on the side wall and is staggered with the sliding door 3 and the temperature control component 5 to avoid interference and provide a larger viewing angle observation area, so as to facilitate timely observation and recording of the cell culture conditions inside the incubator 1.

[0057] A cell culture method comprising the following steps:

[0058] S1, prepare culture medium: basal medium contains DMEM or RPMI1640, supplements contain fetal bovine serum and antibiotics;

[0059] S2, cell inoculation: inoculate the cell suspension into the culture flask to ensure that the cells are evenly distributed in the culture medium;

[0060] S3, setting culture conditions: setting the culture temperature inside the incubator 1 through the temperature control component 5. In the present application, the interior of the incubator 1 is configured as multiple layers of separate culture chambers through a partition plate 221, and different culture conditions can be set. Each layer of the culture chamber is correspondingly provided with a take-out port, and each layer of the separate culture chamber is provided with a temperature control plate 52 and a corresponding temperature sensor, and different culture temperatures can be set; at the same time, a connection setting can also be performed, and multiple layers of separate culture chambers are connected through the control component 7 to form a culture chamber with unified culture conditions;

[0061] S4, placing in culture: open the sliding door 3, place the culture bottle in the culture room 211 through the access port, and place one culture bottle in each culture room 211. After placement, close the sliding door 3, and drive the culture rack 2 to rotate through the control component 7 to culture the cells inside;

[0062] S5, regular liquid replacement: fresh culture medium is replaced every 2-3 days to maintain the growth state of cells. Each time the culture bottle is taken out and placed, only one culture bottle is taken out and placed each time. The partition 4, the culture room 211 and the sliding door 3 together form a partition, which is sealed to avoid affecting the remaining cells inside the incubator 1. After opening the sliding door 3, the culture medium inside is taken out, replaced, and successfully placed. The temperature inside the partition needs to be controlled and adjusted by the temperature control component 5, and because the range of the partition is small, the temperature control can be quickly adjusted to quickly adjust the re-placed culture bottle to a suitable growth environment temperature. When the internal temperature of the culture room 211 to be opened is the same as the internal culture temperature of the incubator 1, the control component 7 can continue to rotate the culture rack 2 to ensure a stable and suitable growth environment for the remaining cells inside. When the culture rooms on different layers have unified culture conditions, when the culture bottles are taken out and placed, the control component 7 can also be used to temporarily isolate and protect the individual culture rooms on each layer, which further plays a role of early warning and prevention, avoiding leakage of the partition and affecting the overall cells of the incubator 1.

[0063] Working principle: In the present application, the culture space inside the incubator 1 is configured as multiple layers of separate culture chambers through a partition plate 221, and different culture conditions can be set. Each layer of the culture chamber is correspondingly provided with a take-out port, and each layer of the separate culture chamber is provided with a temperature control component 5 to adjust its temperature, and different culture temperatures can be set; at the same time, a connection setting can also be performed, and multiple layers of separate culture chambers are connected through a control component 7 to form a culture chamber with uniform culture conditions;

[0064] Each time when taking and placing a culture bottle, only one culture bottle is taken and placed each time, and a partition is formed between the partition 4, the culture room 211 and the sliding door 3. The partition is sealed to avoid affecting the remaining cells in the incubator 1 during opening. After opening the sliding door 3, the culture medium inside is taken out and replaced, and then successfully placed. The temperature inside the partition needs to be controlled and adjusted by the temperature control component 5, and because the range of the partition is small, the temperature control can be quickly adjusted to quickly adjust the re-placed culture bottle to a suitable growth environment temperature. The temperature of the partition is monitored and adjusted by the temperature control component 5 set. When the internal temperature of the culture room 211 to be opened is the same as the internal culture temperature of the incubator 1, the culture rack 2 can continue to be rotated by the control component 7 to ensure a stable and suitable growth environment for the remaining cells inside.

[0065] When the culture conditions of different layers of culture chambers are unified, each layer of individual culture chambers can also be temporarily isolated and protected by the control component 7 when the culture bottles are taken out and placed, which further plays a role of early warning and prevention, avoids leakage in the partitions, and affects the overall cells of the incubator 1, which is beneficial to reduce overall losses.

[0066] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0067] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell culture device, comprising a culture box (1) and a culture rack (2), characterized in that: The culture rack (2) is located in the culture box (1). The culture rack (2) comprises a culture seat (21), a connecting piece (22) and a controllable sealing piece (23). The culture seats (21) are arranged in a linear array in the culture box (1). The connecting piece (22) is arranged between adjacent culture seats (21). The controllable sealing piece (23) is arranged on the connecting piece (22). The culture seats (21) are uniformly provided with culture rooms (211) on the peripheral wall. The side wall of the culture box (1) is uniformly provided with access ports. The access ports and the culture seats (21) are arranged in a one-to-one correspondence. A sliding door (3) is movably installed in the access ports. Partitions (4) are uniformly distributed in the culture box (1). The partitions (4) and the access ports are arranged in a one-to-one correspondence. The partitions (4) and the culture rooms (211) and the sliding door (3) together form a partition. The partitions (4) comprise a fixing frame (41), A telescopic frame (42), a telescopic cavity (43), a spring (44) and a telescopic plate (45), wherein the fixed frame (41) is fixedly mounted on the inner wall of the incubator (1), the telescopic frame (42) is arranged at one end of the fixed frame (41), the telescopic cavity (43) is provided in the fixed frame (41), one end of the telescopic frame (42) extends into the telescopic cavity (43) and is fixedly mounted with the telescopic plate (45), the spring (44) is fixedly mounted between the telescopic plate (45) and the telescopic cavity (43), the spring (44) is evenly distributed in the telescopic cavity (43), the other end of the telescopic frame (42) is provided with a reinforced sealing member (8), the outer wall of the incubator (1) is provided with a temperature control component (5), the temperature control component (5) performs temperature control on the incubator (1) and the partition, and the lower end of the incubator (1) is provided with a driving member (6), the driving member (6) drives the incubator rack (2) to rotate.

2. A cell culture device according to claim 1, characterized in that: The connecting member (22) comprises a partition plate 1 (221) and a connecting shaft (222); the partition plate 1 (221) is fixedly mounted on the culture box (1); the connecting shaft (222) is movably mounted at a middle position of the partition plate 1 (221); the upper and lower ends of the connecting shaft (222) are fixedly connected to the culture seat (21) via fastening bolts respectively; a movable groove (2211) is provided in the partition plate 1 (221); and the controllable sealing member (23) is arranged in the movable groove (2211).

3. A cell culture device according to claim 2, characterized in that: The controllable sealing member (23) comprises a partition plate 2 (231), an arc-shaped ventilation groove 1 (232) and an arc-shaped ventilation groove 2 (233); the partition plate 2 (231) is movably mounted in the movable groove (2211) via a control component (7); the arc-shaped ventilation groove 1 (232) and the arc-shaped ventilation groove 2 (233) are respectively arranged through the partition plate 1 (221) and the partition plate 2 (231); the arc-shaped ventilation groove 1 (232) and the arc-shaped ventilation groove 2 (233) are respectively arranged in a linear array on the partition plate 1 (221) and the partition plate 2 (231); the arc-shaped ventilation groove 1 (232) and the arc-shaped ventilation groove 2 (233) are arranged in a one-to-one correspondence; the arc-shaped ventilation groove 1 (232) and the arc-shaped ventilation groove 2 (233) are evenly distributed around the circumference.

4. A cell culture device according to claim 3, characterized in that: The control component (7) comprises a driving motor (71), a half-toothed gear (72), a reciprocating groove (73) and a rack (74); the driving motor (71) is fixedly mounted on the partition plate (221); the reciprocating groove (73) is arranged at the upper end of the partition plate (231); the output end of the driving motor (71) extends into the reciprocating groove (73) and is fixedly mounted with the half-toothed gear (72); the rack (74) is symmetrically fixedly mounted on the side wall of the reciprocating groove (73); and the half-toothed gear (72) can and can only mesh with the rack (74) at one end at the same time.

5. A cell culture device according to claim 1, characterized in that: The reinforced seal (8) comprises a sealing strip (81), a roller (82), a sealing groove (83) and a groove (84); the sealing strip (81) is fixedly mounted on the other end of the telescopic frame (42); the roller (82) is arranged at the four corners of the sealing strip (81); the sealing groove (83) and the culture room (211) are arranged in a one-to-one correspondence; the sealing strip (81) and the sealing groove (83) match each other; the roller (82) is arranged at the four corners of the sealing strip (81); the groove (84) is arranged at the four corners of the sealing groove (83); the roller (82) is movably mounted in the corresponding groove (84); and the left and right groove bodies of adjacent sealing grooves (83) are arranged overlappingly.

6. A cell culture device according to claim 1, characterized in that: The temperature control assembly (5) comprises a temperature control seat (51), a first temperature control plate (52) and a second temperature control plate (53); the temperature control seat (51) is fixedly mounted on the outer wall of the incubator (1); the first temperature control plate (52) is arranged on the inner wall of the incubator (1); the second temperature control plate (53) is arranged on the inner wall of the fixed frame (41); and the temperature control seat (51) is electrically connected to the first temperature control plate (52) and the second temperature control plate (53) respectively via wires.

7. A cell culture device according to claim 2, characterized in that: The driving member (6) comprises a second driving motor (61), a driving gear (62) and a driven gear (63); the second driving motor (61) is fixedly mounted at the lower edge of the incubator (1); the driving gear (62) is fixedly mounted at the output end of the second driving motor (61); the lower end of the lowermost connecting shaft (222) extends out of the incubator (1) and is fixedly mounted with the driven gear (63); the driving gear (62) and the driven gear (63) are meshed.

8. A cell culture device according to claim 1, characterized in that: The incubator (1) comprises a top cover (11), a box body (12), a bottom plate (13) and supporting legs (14); the top cover (11), the box body (12), the bottom plate (13) and the supporting legs (14) are arranged in sequence from top to bottom; the top cover (11), the box body (12) and the bottom plate (13) are respectively fixedly connected by fastening bolts; the supporting legs (14) are evenly arranged in a circular shape at the lower end of the bottom plate (13); a transparent observation window (9) is provided on a side wall of the box body (12); and the transparent observation windows (9) are arranged on both sides of the box body (12) in a mirror-symmetrical manner.

9. A cell culture method according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1, prepare culture medium: basal medium contains DMEM or RPMI1640, supplements contain fetal bovine serum and antibiotics; S2, cell inoculation: inoculate the cell suspension into the culture flask to ensure that the cells are evenly distributed in the culture medium; S3, setting culture conditions: setting the culture temperature inside the culture box 1 by means of the temperature control component (5). In the present application, the interior of the culture box (1) is configured as multiple layers of separate culture chambers by means of a partition plate (221), and different culture conditions can be set. Each layer of the culture chamber is provided with a corresponding access port. Each layer of the separate culture chamber is provided with a temperature control plate (52) and a corresponding temperature sensor, and different culture temperatures can be set. At the same time, a connection setting can also be performed, and multiple layers of separate culture chambers can be connected by means of a control component (7) to form a culture chamber with uniform culture conditions. S4, placing in culture: opening the sliding door (3), placing the culture bottle in the culture room (211) through the access opening, with one culture bottle placed in each culture room (211). After placement, closing the sliding door (3), driving the culture rack (2) to rotate through the control component (7), and culturing the cells inside; S5, regular liquid replacement: fresh culture medium is replaced every 2-3 days to maintain the growth state of the cells. When taking and placing the culture bottle, only one culture bottle is taken and placed at a time. The partition (4), the culture room (211) and the sliding door (3) together form a partition. The partition is sealed to avoid affecting the remaining cells in the incubator (1). After opening the sliding door (3), the culture medium inside is taken out and replaced. After it is successfully placed, the temperature inside the partition needs to be controlled and adjusted by the temperature control component (5). Since the range of the partition is small, it can be quickly adjusted. Temperature control is used to quickly adjust the re-placed culture bottles to a suitable growth environment temperature. When the internal temperature of the culture room (211) to be opened is the same as the internal culture temperature of the incubator (1), the culture rack (2) can be continuously rotated by the control component (7), thereby ensuring a stable and suitable growth environment for the remaining cells inside. When the culture rooms on different layers have the same culture conditions, the control component (7) can also be used to temporarily isolate and protect the individual culture rooms on each layer when the culture bottles are taken out and placed, thereby further playing a role of early warning and prevention, thereby avoiding leakage in the partition room and affecting the overall cells in the incubator (1).

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