Additive for preventing and treating pig limb and hoof diseases, pig feed, preparation method and application

Abstract

The present invention discloses an additive for preventing and treating pig limb and hoof disease. The raw materials for preparing the additive include at least a composite bacterial fermentation liquid and a traditional Chinese medicine composition; the composite bacterial fermentation liquid includes a Bacillus subtilis fermentation liquid, a Lactobacillus lactis fermentation liquid and a Saccharomyces cerevisiae fermentation liquid; the traditional Chinese medicine composition includes at least psoralea corylifolia, Atractylodes macrocephala, Poria cocos, frankincense, Curcuma aromatica and Rehmannia glutinosa. The present invention removes or weakens the bitterness of the medicinal materials by fermenting the traditional Chinese medicine with microorganisms, thereby improving the taste of the traditional Chinese medicine after adding it to pig feed and avoiding adverse effects on the pig's feed intake; more importantly, the present invention synergistically compounds the probiotics and the traditional Chinese medicine composition, so that the present invention can prevent and treat mild cases of nutritional limb and hoof disease and bacterial limb and hoof disease. The present invention also discloses a preparation method of the additive, pig feed containing the additive and application.

Description

Technical Field

[0001] The invention belongs to the technical field of pig breeding, and particularly relates to an additive for preventing and treating pig limb and hoof diseases. The invention also relates to pig feed containing the additive, a preparation method of the additive and application of the additive. Background Art

[0002] Pig hoof and limb disease is a general term for a type of disease, which generally refers to movement disorders in the pig's hooves and limbs or ulcers, cracks, erosions and other symptoms in the hooves and limbs. It mainly includes nutritional hoof and limb diseases, bacterial hoof and limb diseases, viral hoof and limb diseases, hereditary hoof and limb diseases, etc.

[0003] It is difficult for infected pigs to carry out normal daily activities (such as eating and walking), and treatment is not effective when symptoms are severe. If it is a contagious bacterial limb and hoof disease, it may also infect other healthy pigs, which has a negative impact on the production efficiency of the pig herd. Pig limb and hoof disease has become one of the important reasons for the elimination of breeding pigs in large-scale pig farms, seriously damaging the economic benefits of the pig farming industry. Viral limb and hoof diseases progress quickly, and hereditary limb and hoof diseases are genetic problems. They are more difficult to prevent in daily feeding or to treat in time when the symptoms are mild. However, for nutritional limb and hoof diseases and bacterial limb and hoof diseases, prevention is more important than treatment and has a higher cost-effectiveness.

[0004] In order to effectively prevent pig limb and hoof diseases, the patent document with announcement number CN113040283B discloses a premix feed and its application for protecting the health of sows' limbs and hooves. The main ingredients of the premix feed are manganese gluconate, zinc lactate, copper citrate, phytase and rice husk powder. The premix feed can significantly reduce the incidence of sows' heel morbidity and the incidence of the heel-sole junction.

[0005] The patent document with announcement number CN109924342B discloses a compound feed, a compound feed additive and a method for preparing a compound feed additive for reducing limb and hoof diseases in sows, comprising: sintering dried rice husks in an oxygen-free environment to form carbonized rice husks; then adding the carbonized rice husks to a compound enzyme preparation for enzymolysis, taking them out and draining them, then pouring them into a compound trace element aqueous solution for soaking, then taking them out for freeze-drying and crushing them, and after the crushing is completed, adding calcium dihydrogen phosphate and calcium lactate and mixing them evenly to obtain a first material; crushing green whole-plant corn and then mixing them evenly with a fermentation agent and then sealing and fermenting them, and after the fermentation is completed, adding the first material, alfalfa powder and compound vitamins and mixing them evenly, then freeze-drying and crushing them, and then adding carboxymethyl dextran and sodium tripolyphosphate and mixing them evenly to obtain a compound feed additive. The compound feed additive can effectively reduce the occurrence of limb and hoof diseases in sows.

[0006] However, the above-mentioned existing technologies primarily focus on formulating nutritional elements such as vitamins and minerals, which have a good preventive and therapeutic effect on pigs suffering from nutritional limb and hoof diseases, but are more limited in their effectiveness against bacterial limb and hoof diseases. Furthermore, the overuse of antibiotics on pig farms is a serious problem, making it inappropriate to use antibiotics for prevention or treatment during the early stages of symptoms.

[0007] The applicant has deep roots in the field of animal husbandry technology and has an in-depth understanding of downstream farms. He believes that it is necessary to develop an antibiotic-free additive that can simultaneously prevent and treat nutritional and bacterial limb and hoof diseases, and apply it to daily feeding in farms. Summary of the Invention

[0008] In response to the problems in the related art, the present invention proposes an additive for preventing and treating pig limb and hoof diseases to overcome the above-mentioned technical problems existing in the existing related art; the present invention also discloses a preparation method of the additive, pig feed containing the additive, and application.

[0009] The technical solution of the present invention is achieved as follows:

[0010] An additive for preventing and treating pig limb and hoof diseases, wherein the raw materials for preparation include at least a composite bacterial fermentation liquid and a traditional Chinese medicine composition;

[0011] The composite bacterial fermentation liquid comprises Bacillus subtilis fermentation liquid, Lactobacillus lactis fermentation liquid and Saccharomyces cerevisiae fermentation liquid;

[0012] The traditional Chinese medicine composition at least comprises psoralea corylifolia, atractylodes macrocephala, tuckahoe, frankincense, turmeric and rehmannia root.

[0013] Compared with the existing technology, the present invention can remove or weaken the bitterness of Chinese medicine through microbial fermentation, thereby improving the taste of Chinese medicine after adding it to pig feed and avoiding adverse effects on pigs' feed intake; more importantly, the present invention combines probiotics and Chinese medicine compositions, so that the present invention can prevent and treat mild cases of nutritional limb and hoof diseases and bacterial limb and hoof diseases.

[0014] Preferably, the weight ratio of the Bacillus subtilis fermentation broth, the Lactobacillus lactis fermentation broth and the Saccharomyces cerevisiae fermentation broth is 0.8-1.0:1.0-1.2:0.8-1.0.

[0015] Preferably, the Chinese medicine composition comprises at least the following raw materials in parts by weight:

[0016] 40-70 parts of Psoralea corylifolia, 40-60 parts of Atractylodes macrocephala, 40-60 parts of Poria cocos, 30-50 parts of Frankincense, 30-50 parts of Curcuma aromatica and 30-50 parts of Rehmannia root.

[0017] More preferably, the Chinese medicine composition comprises the following raw materials in parts by weight:

[0018] 55-70 parts of Psoralea corylifolia, 45-53 parts of Atractylodes macrocephala, 45-53 parts of Poria cocos, 35-43 parts of Frankincense, 35-43 parts of Curcuma aromatica, 35-43 parts of Rehmannia root, 20-33 parts of Dipsacus root, 20-33 parts of Alisma orientalis, 20-33 parts of White Peony Root, 20-33 parts of Myrrha and 20-33 parts of Licorice root.

[0019] From the perspective of traditional Chinese medicine, the spleen governs the limbs and muscles, so spleen damage will cause swelling or damage to the joints and muscles of the pig's limbs. The spleen is the foundation of acquired constitution. If the spleen is not functioning well, the body's energy supply will be insufficient. The kidney governs the bones and reproductive system, which is the foundation of congenital constitution. During pregnancy and childbirth, sows will continuously consume essence and kidney energy, which will have adverse effects on bones and hoofs. The liver governs tendons, and the kidney is water, and the liver is wood. Water produces wood. When the kidney water is insufficient, the liver wood is affected, and then the tendons of the limbs will have adverse symptoms. Therefore, sows will have difficulty standing and have diarrhea. Therefore, the present invention focuses on the design of Chinese medicine formulas from the aspects of strengthening the spleen, consolidating the kidneys, and soothing the liver, so as to achieve the effect of strengthening bones and preventing pig limb and hoof diseases. Specifically, Psoralea corylifolia, Dipsacus root and Rehmannia root are mainly used to tonify the kidneys and strengthen the kidneys, and then have a bone strengthening effect on sows. The main functions of Atractylodes macrocephala, Poria cocos, Alisma orientalis and White Peony Root are to strengthen the spleen and eliminate dampness, so that sows have better nutrient absorption efficiency; Frankincense, Curcuma and Myrrh work together to soothe the liver and strengthen the tendons, while relieving pain and promoting muscle regeneration, thereby reducing the pain of pigs with limb and hoof diseases; Licorice is mainly used to harmonize the various medicines. Through the above Chinese medicines, the overall nutrient absorption capacity and immune capacity of the sow are enhanced, the tendons and bones are strengthened, and it is suitable for the prevention and mild treatment of nutritional limb and hoof diseases and bacterial limb and hoof diseases.

[0020] The preparation method of the above-mentioned additive for preventing and treating pig limb and hoof diseases comprises the following steps:

[0021] 1) reviving and culturing Bacillus subtilis, Lactobacillus lactis and Saccharomyces cerevisiae to obtain Bacillus subtilis fermentation broth, Lactobacillus lactis fermentation broth and Saccharomyces cerevisiae fermentation broth;

[0022] 2) mixing Bacillus subtilis fermentation broth, Lactobacillus lactis fermentation broth and Saccharomyces cerevisiae fermentation broth in a preset weight ratio to produce a composite bacterial fermentation broth;

[0023] 3) preparing the Chinese medicine composition according to the preset components and weight proportions, and crushing the mixture;

[0024] 4) preparing fermentation raw materials and performing fermentation, wherein the fermentation raw materials at least include the composite bacterial fermentation liquid and the traditional Chinese medicine composition;

[0025] 5) After the fermentation is completed, the mixture is bottled to obtain an additive for preventing and treating pig limb and hoof diseases.

[0026] Preferably, step 1) includes the following steps:

[0027] 1-1) Bacillus subtilis, Lactobacillus lactis, and Saccharomyces cerevisiae were inoculated into the appropriate culture medium, and the shaking incubator was set at a temperature of 30-37°C and a speed of 120-180 rpm / min to revitalize the culture until the viable cell count was ≥0.8×10 8 CFU / ml, as the primary seed solution;

[0028] 1-2) Transfer each first-level seed solution to a fermentation tank with an inoculation amount of 5-8% of the total mass of the fermentation product, culture temperature at 25-30°C, stirring speed at 200-250 rpm, and ferment until the bacterial content is ≥0.8×10 8 CFU / ml, as the secondary seed solution;

[0029] 1-3) Transfer each secondary seed solution to the production tank, inoculate it at a rate of 10-15% of the total mass of the fermentation product, culture at a temperature of 25-30°C, stir at a speed of 120-180 rpm, and ferment until the bacterial count is ≥1.0×10 8 CFU / ml, the fermentation is completed, and Bacillus subtilis fermentation broth, Lactobacillus lactis fermentation broth and Saccharomyces cerevisiae fermentation broth are obtained respectively.

[0030] Preferably, in step 1-2), the fermentation product in the fermentation tank comprises the following raw materials in the following mass percentages:

[0031] Soybean meal hydrolyzate: 5-8wt%;

[0032] Glucose: 1-3 wt%;

[0033] Corn steep liquor: 1-3 wt%;

[0034] Disodium hydrogen phosphate: 0.1-0.5wt%;

[0035] Manganese sulfate or manganese acid phosphate: 0.1-0.5wt%;

[0036] Magnesium sulfate: 0.01-0.05wt%;

[0037] Zinc sulfate: 0.01-0.05wt%;

[0038] Copper sulfate: 0.01-0.05wt%;

[0039] The balance is water.

[0040] Preferably, in step 1-3), the fermentation product in the production tank comprises the following raw materials in the following mass percentages:

[0041] Soybean meal hydrolyzate: 10-12wt%;

[0042] Glucose: 3-5wt%;

[0043] Corn steep liquor: 3-5wt%;

[0044] Disodium hydrogen phosphate: 0.1-0.5wt%;

[0045] Manganese sulfate or manganese acid phosphate: 0.1-0.5wt%;

[0046] Magnesium sulfate: 0.01-0.05wt%;

[0047] Zinc sulfate: 0.01-0.05wt%;

[0048] Copper sulfate: 0.01-0.05wt%;

[0049] The balance is water.

[0050] Preferably, in step 3), the traditional Chinese medicine composition is crushed to 300-500 mesh.

[0051] Preferably, in step 4), the fermentation raw materials contain at least 100 parts by weight of the traditional Chinese medicine composition and 8 to 12 parts by weight of the composite bacterial fermentation liquid.

[0052] More preferably, in step 4), the fermentation raw material comprises the following components in parts by weight:

[0053] 100 parts of crushed Chinese medicine composition,

[0054] 8 to 12 parts of compound bacterial fermentation liquid,

[0055] 20-25 portions of wheat bran,

[0056] 5-10 parts soybean meal,

[0057] 2.5-8.0 parts calcium carbonate,

[0058] 0.4-0.6 parts tryptophan

[0059] 0.1-0.5 parts of ferrous sulfate,

[0060] 0.1-0.3 parts of zinc sulfate,

[0061] 0.05-0.1 parts of manganese sulfate or manganese acid phosphate,

[0062] 0.05-0.1 parts of magnesium sulfate,

[0063] 0.05-0.1 parts of copper sulfate,

[0064] 0.01-0.05 parts of disodium hydrogen phosphate.

[0065] The probiotics, vitamins and Chinese medicine composition are synergistically compounded to further improve the effect of the present invention on preventing and treating nutritional limb and hoof diseases and bacterial limb and hoof diseases and mild symptoms.

[0066] Preferably, in step 4), water is added to the fermentation raw materials to adjust the water content to 50-60%, the pH value is adjusted to 5.5-7.0, the fermentation temperature is 25-30° C., and the fermentation time is 96-120 h;

[0067] Stir at least twice every 24 hours, each time for 8 to 10 minutes, when the total bacterial count reaches ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

[0068] The present invention also discloses a pig feed comprising the above additive, wherein the additive is added in an amount of 1 to 3 kg per ton of pig feed.

[0069] The invention also discloses application of the additive for preventing and treating pig limb and hoof diseases in preventing and treating nutritional limb and hoof diseases and bacterial limb and hoof diseases. DETAILED DESCRIPTION

[0070] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0071] Unless otherwise specified, the following materials are common commercially available materials.

[0072] Example 1

[0073] 1-1) Inoculate Bacillus subtilis (strain number CGMCC1.3358) into BPY medium;

[0074] Lactobacillus lactis (strain number CGMCC 1.1880) was inoculated into MRS medium;

[0075] Saccharomyces cerevisiae (strain number CGMCC 2.1554) was inoculated into PDA medium;

[0076] The shaker was set at 30°C and 120 rpm / min for culture revival until the viable bacterial count was ≥ 0.8 × 10 8 CFU / ml, used as the primary seed solution.

[0077] 1-2) Transfer each first-level seed solution to a fermenter with an inoculation amount of 5% of the total mass of the fermentation product, culture temperature at 25°C, stirring speed at 200 rpm, and ferment until the bacterial content is ≥ 0.8 × 10 8 CFU / ml, used as secondary seed solution.

[0078] The fermentation in the fermenter contains the following qualities of raw materials:

[0079] Soybean meal hydrolyzate: 5 kg (Hubei Xinrunde Chemical Co., Ltd.);

[0080] Glucose: 1kg;

[0081] Corn steep liquor: 1kg;

[0082] Disodium hydrogen phosphate: 0.1kg;

[0083] Manganese sulfate / manganese acid phosphate: 0.1kg;

[0084] Magnesium sulfate: 0.01kg;

[0085] Zinc sulfate: 0.01kg;

[0086] Copper sulfate: 0.01kg;

[0087] Add water to 100 kg.

[0088] 1-3) Transfer each secondary seed solution to the production tank, inoculate at 10% of the total mass of the fermentation product, culture at 25°C, stir at 120 rpm, and ferment until the bacterial count is ≥1.0×10 8 CFU / ml, the fermentation is completed to obtain Bacillus subtilis fermentation broth, Lactobacillus lactis fermentation broth and Saccharomyces cerevisiae fermentation broth.

[0089] The fermentation in the production tanks contains the following raw materials of the following qualities:

[0090] Soybean meal hydrolyzate: 10 kg (Hubei Xinrunde Chemical Co., Ltd.);

[0091] Glucose: 3kg;

[0092] Corn steep liquor: 3kg;

[0093] Disodium hydrogen phosphate: 0.1kg;

[0094] Manganese sulfate: 0.1kg;

[0095] Magnesium sulfate: 0.01kg;

[0096] Zinc sulfate: 0.01kg;

[0097] Copper sulfate: 0.01kg;

[0098] Add water to 100 kg.

[0099] 2) The Bacillus subtilis fermentation liquid, the Lactobacillus lactis fermentation liquid and the Saccharomyces cerevisiae fermentation liquid were mixed in a weight ratio of 0.8:1.0:0.8 to prepare a composite bacterial fermentation liquid.

[0100] 3) Prepare the following Chinese medicine composition by weight:

[0101] 55kg of Psoralea corylifolia, 45kg of Atractylodes macrocephala, 45kg of Poria cocos, 35kg of Frankincense, 35kg of Curcuma zedoaria, 35kg of Rehmannia root, 20kg of Dipsacus root, 20kg of Alisma orientalis, 20kg of White Peony Root, 20kg of Myrrha and 20kg of Licorice root.

[0102] The above-mentioned Chinese medicine composition is ultrafinely ground to 300 mesh.

[0103] 4) Prepare the following fermentation raw materials by weight:

[0104] 10kg of crushed Chinese medicine composition,

[0105] 0.8kg composite bacterial fermentation liquid,

[0106] 2kg wheat bran,

[0107] 0.5kg soybean meal,

[0108] 0.25kg calcium carbonate,

[0109] 40g tryptophan,

[0110] 10g ferrous sulfate,

[0111] 10g zinc sulfate,

[0112] 5g manganese sulfate,

[0113] 5g magnesium sulfate,

[0114] 5g copper sulfate,

[0115] 1g disodium hydrogen phosphate.

[0116] The fermentation raw materials were placed in a fermentation tank, water was added to make the water content 50%, the pH value was adjusted to 5.5, the fermentation temperature was controlled at 25-30 °C, and the fermentation time was 96 h; the fermentation was stirred twice every 24 h, each time for 8 min, and the total bacterial count was ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

[0117] 5) Filling into a specification of 25 kg / barrel and storing in a ventilated and cool place to prepare an additive for preventing limb and hoof diseases of sows.

[0118] Example 2

[0119] 1-1) Inoculate Bacillus subtilis (strain number CGMCC1.3358) into BPY medium;

[0120] Lactobacillus lactis (strain number CGMCC 1.1880) was inoculated into MRS medium;

[0121] Saccharomyces cerevisiae (strain number CGMCC 2.1554) was inoculated into PDA medium;

[0122] The shaker was set at 35°C and 150 rpm / min for revival until the viable bacterial count was ≥ 0.8 × 10 8 CFU / ml, used as the primary seed solution.

[0123] 1-2) Transfer each first-level seed solution to a fermentation tank with an inoculation amount of 6% of the total mass of the fermentation product, culture temperature at 28°C, stirring speed at 225 rpm, and ferment until the bacterial content is ≥ 0.8 × 10 8 CFU / ml, used as secondary seed solution.

[0124] The fermentation in the fermenter contains the following qualities of raw materials:

[0125] Soybean meal hydrolyzate: 7 kg (Hubei Xinrunde Chemical Co., Ltd.);

[0126] Glucose: 2kg;

[0127] Corn steep liquor: 2kg;

[0128] Disodium hydrogen phosphate: 0.3kg;

[0129] Manganese sulfate / manganese acid phosphate: 0.3kg;

[0130] Magnesium sulfate: 0.03kg;

[0131] Zinc sulfate: 0.03kg;

[0132] Copper sulfate: 0.03kg;

[0133] Add water to 100 kg.

[0134] 1-3) Each secondary seed solution was transferred to a production tank with an inoculation amount of 12% of the total mass of the fermentation product, culture temperature of 27°C, stirring speed of 150 rpm, and fermentation until the bacterial content was ≥1.0×10 8 CFU / ml, the fermentation is completed to obtain Bacillus subtilis fermentation broth, Lactobacillus lactis fermentation broth and Saccharomyces cerevisiae fermentation broth.

[0135] The fermentation in the production tanks contains the following raw materials of the following qualities:

[0136] Soybean meal hydrolyzate: 11 kg (Hubei Xinrunde Chemical Co., Ltd.);

[0137] Glucose: 4 kg;

[0138] Corn steep liquor: 4kg;

[0139] Disodium hydrogen phosphate: 0.3kg;

[0140] Manganese acid phosphate: 0.3kg;

[0141] Magnesium sulfate: 0.03kg;

[0142] Zinc sulfate: 0.03kg;

[0143] Copper sulfate: 0.03kg;

[0144] Add water to 100 kg.

[0145] 2) The Bacillus subtilis fermentation liquid, the Lactobacillus lactis fermentation liquid and the Saccharomyces cerevisiae fermentation liquid were mixed in a weight ratio of 0.9:1.1:0.9 to prepare a composite bacterial fermentation liquid.

[0146] 3) Prepare the following Chinese medicine composition by weight:

[0147] 65kg of Psoralea corylifolia, 50kg of Atractylodes macrocephala, 48kg of Poria cocos, 40kg of Frankincense, 40kg of Curcuma zedoaria, 38kg of Rehmannia root, 25kg of Dipsacus root, 28kg of Alisma orientalis, 25kg of White Peony Root, 25kg of Myrrha and 30kg of Licorice root.

[0148] The above-mentioned Chinese medicine composition is ultrafinely ground to 400 mesh.

[0149] 4) Prepare the following fermentation raw materials by weight:

[0150] 10kg of crushed Chinese medicine composition,

[0151] 1kg composite bacterial fermentation liquid,

[0152] 2.2kg wheat bran,

[0153] 0.8kg soybean meal,

[0154] 0.5kg calcium carbonate,

[0155] 50g tryptophan,

[0156] 30g ferrous sulfate,

[0157] 20g zinc sulfate,

[0158] 8g manganese acid phosphate,

[0159] 8g magnesium sulfate,

[0160] 8g copper sulfate,

[0161] 3g disodium hydrogen phosphate.

[0162] The fermentation raw materials were placed in a fermentation tank, water was added to make the water content 55%, the pH value was adjusted to 6.0, the fermentation temperature was controlled at 25-30 °C, and the fermentation time was 100 h; the fermentation was stirred 3 times every 24 h, each time for 9 min, and the total bacterial count was ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

[0163] 5) Filling into a specification of 25 kg / barrel and storing in a ventilated and cool place to prepare an additive for preventing limb and hoof diseases of sows.

[0164] Example 3

[0165] 1-1) Inoculate Bacillus subtilis (strain number CGMCC1.3358) into BPY medium;

[0166] Lactobacillus lactis (strain number CGMCC 1.1880) was inoculated into MRS medium;

[0167] Saccharomyces cerevisiae (strain number CGMCC 2.1554) was inoculated into PDA medium;

[0168] The shaker was set at 37°C and 180 rpm / min for culture revival until the viable bacterial count was ≥ 0.8 × 10 8 CFU / ml, used as the primary seed solution.

[0169] 1-2) Transfer each first-level seed solution to a fermentation tank with an inoculation amount of 8% of the total mass of the fermentation product, culture temperature at 30°C, stirring speed at 250 rpm, and ferment until the bacterial content is ≥ 0.8 × 10 8 CFU / ml, used as secondary seed solution.

[0170] The fermentation in the fermenter contains the following qualities of raw materials:

[0171] Soybean meal hydrolyzate: 8 kg (Hubei Xinrunde Chemical Co., Ltd.);

[0172] Glucose: 3kg;

[0173] Corn steep liquor: 3kg;

[0174] Disodium hydrogen phosphate: 0.5kg;

[0175] Manganese sulfate: 0.5kg;

[0176] Magnesium sulfate: 0.05kg;

[0177] Zinc sulfate: 0.05kg;

[0178] Copper sulfate: 0.05kg;

[0179] Add water to 100 kg.

[0180] 1-3) Transfer each secondary seed solution to the production tank, inoculate at 15% of the total mass of the fermentation product, culture at 30°C, stir at 180 rpm, and ferment until the bacterial count is ≥1.0×10 8 CFU / ml, the fermentation is completed to obtain Bacillus subtilis fermentation broth, Lactobacillus lactis fermentation broth and Saccharomyces cerevisiae fermentation broth.

[0181] The fermentation in the production tanks contains the following raw materials of the following qualities:

[0182] Soybean meal hydrolyzate: 12 kg (Hubei Xinrunde Chemical Co., Ltd.);

[0183] Glucose: 5kg;

[0184] Corn steep liquor: 5kg;

[0185] Disodium hydrogen phosphate: 0.5kg;

[0186] Manganese sulfate: 0.5kg;

[0187] Magnesium sulfate: 0.05kg;

[0188] Zinc sulfate: 0.05kg;

[0189] Copper sulfate: 0.05kg;

[0190] Add water to 100 kg.

[0191] 2) The Bacillus subtilis fermentation liquid, the Lactobacillus lactis fermentation liquid and the Saccharomyces cerevisiae fermentation liquid were mixed in a weight ratio of 1.0:1.2:1.0 to prepare a composite bacterial fermentation liquid.

[0192] 3) Prepare the following Chinese medicine composition by weight:

[0193] 70kg of Psoralea corylifolia, 53kg of Atractylodes macrocephala, 53kg of Poria cocos, 43kg of Frankincense, 43kg of Curcuma zedoaria, 43kg of Rehmannia root, 33kg of Dipsacus root, 33kg of Alisma orientalis, 33kg of White Peony Root, 33kg of Myrrha and 33kg of Licorice.

[0194] The above-mentioned Chinese medicine composition is ultrafinely ground to 500 mesh.

[0195] 4) Prepare the following fermentation raw materials by weight:

[0196] 10kg of crushed Chinese medicine composition,

[0197] 1.2kg composite bacterial fermentation liquid,

[0198] 2.5kg wheat bran,

[0199] 1kg soybean meal,

[0200] 0.8kg calcium carbonate,

[0201] 60g tryptophan,

[0202] 50g ferrous sulfate,

[0203] 30g zinc sulfate,

[0204] 10g manganese sulfate,

[0205] 10g magnesium sulfate,

[0206] 10g copper sulfate,

[0207] 5g disodium hydrogen phosphate.

[0208] The fermentation raw materials were placed in a fermentation tank, water was added to make the water content 60%, the pH value was adjusted to 7.0, the fermentation temperature was controlled at 25-30 °C, and the fermentation time was 120 h; the fermentation was stirred 4 times every 24 h, each time for 10 min, and the total bacterial count was ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

[0209] 5) filling into a specification of 25 kg / barrel and storing in a ventilated and cool place to prepare an additive for preventing limb and hoof diseases of sows.

[0210] Comparative Example 1

[0211] "Super Vita", a commercially available product for the prevention and treatment of limb and hoof diseases.

[0212] Comparative Example 2

[0213] Compared with Example 2, this comparative example comprises the following raw materials by weight:

[0214] 2.2kg wheat bran,

[0215] 0.8kg soybean meal,

[0216] 0.5kg calcium carbonate,

[0217] 50g tryptophan

[0218] 30g ferrous sulfate:

[0219] 20g zinc sulfate:

[0220] 8g manganese sulfate / manganese acid phosphate:

[0221] 8g magnesium sulfate:

[0222] 8g copper sulfate;

[0223] 3g disodium hydrogen phosphate.

[0224] That is, this comparative example does not include a fermented Chinese medicine composition and related production steps.

[0225] (1) In vitro antibacterial experiment

[0226] Apply to and order Streptococcus suis, Haemophilus parasuis, Erysipelothrix rhizogenes, Staphylococcus aureus and Corynebacterium pyogenes from the National Veterinary Microbial Bacteria (Virus) Collection Center, and inoculate them onto the culture medium.

[0227] Take appropriate amounts of Examples 1 to 3 and Comparative Example 2 and centrifuge them. After centrifugation, take equal amounts of the supernatant and add them to the culture medium of each pathogenic bacteria. Incubate at 37°C for 12 hours, measure and record the diameter d of the inhibition zone. The standard is as follows:

[0228] Sensitivity Highly sensitive Moderately sensitive Low sensitivity Insensitive d / mm ＞20 15～20 10～15 ＜10

[0229] The results are as follows:

[0230] pathogenic bacteria Streptococcus suis Haemophilus parasuis Erysipelothrix rhizogenes staphylococcus Corynebacterium pyogenes Example 1 Moderately sensitive Moderately sensitive Low sensitivity Highly sensitive Highly sensitive Example 2 Highly sensitive Highly sensitive Moderately sensitive Highly sensitive Highly sensitive Example 3 Highly sensitive Moderately sensitive Moderately sensitive Highly sensitive Highly sensitive Comparative Example 2 Insensitive Insensitive Insensitive Insensitive Insensitive

[0231] The two types of experimental pigs involved in the following experimental projects were provided by Guangzhou Yangcheng Pig Industry Co., Ltd.

[0232] (2) Sixty sows in early pregnancy were randomly divided into six groups, with 10 sows in each group. The sows were in similar body condition and had healthy limbs and hooves. The groups were numbered EG1 to EG3 and CG1 to CG3.

[0233] Groups EG1 to EG3 were added with Examples 1 to 3 in their feed, respectively, at a ratio of 2 kg per ton of feed;

[0234] Groups CG1 and CG2 were added with Comparative Example 1 and Comparative Example 2, respectively, in the feed at a ratio of 2 kg per ton of feed;

[0235] There was no additive in the feed of group CG3.

[0236] The trial period was three months. The initial body weight, backfat thickness and hoof keratin hardness of each experimental pig were measured when it was enrolled in the group. Then, they were measured and recorded again on the 1st and 15th of each month. The food intake of each experimental pig was recorded every day for three months and the occurrence of limb and hoof disease symptoms was observed.

[0237] All experimental pigs were fed three times a day with free access to feed and water. The breeding area was kept clean, hygienic, and well-ventilated.

[0238] (2-1) Backfat measurement and results

[0239] The initial backfat thickness of each test pig was measured by a backfat meter using the P2 point method. The backfat thickness was then measured and recorded again on the 1st and 15th of each month for three months.

[0240] If the final backfat thickness of each test pig fluctuates within ±1 mm relative to the initial backfat thickness, it is considered that there is no significant change in backfat thickness. If the fluctuation range exceeds this, it is considered that the backfat thickness has increased or decreased.

[0241] Group Number of pigs with increased backfat thickness No significant change in backfat thickness Number of pigs with reduced backfat thickness EG1 8 2 0 EG 2 10 0 0 EG 3 9 1 0 CG 1 6 2 2 CG 2 4 3 3 CG 3 2 3 5

[0242] (2-2) Recording and tracking appetite and weight

[0243] Record the initial daily food intake of each experimental pig. A change in food intake within ±0.5 kg or an increase in food intake >0.5 kg was considered to indicate no decrease in appetite; an increase below this fluctuation range was considered to indicate a decrease in appetite.

[0244] Record the initial weight of each test pig. Measure and record the pig's weight on the morning of the 1st and 15th of each month, before eating. If the final weight of each test pig increases by at least 10 kg compared to the initial weight, it is considered healthy and has normal nutrient absorption efficiency. Otherwise, it is considered abnormal and has low nutrient absorption efficiency.

[0245]

[0246] (2-3) Measurement and tracking of pig hoof keratin hardness

[0247] The horny tissue of the hoof bottom of the test pig was collected, cleaned, and trimmed into 1cm pieces with a roughly uniform size. 2 The hardness of pig hoof keratin was tested using a Leeb hardness tester.

[0248] The data of each measurement were compared with the initial hoof keratin hardness of the test sows. Hardness values ​​within the range of ±1.5 were recorded as no significant change, and hardness values ​​outside this range were recorded as an increase or decrease.

[0249]

[0250] (2-4) Statistics on the number of pigs with limb and hoof disease symptoms during the 3-month trial period

[0251] Group EG 1 EG 2 EG 3 CG 1 CG 2 CG 3 Number of animals with limb and hoof symptoms 1 0 0 2 2 4

[0252] The above data show that: compared with CG 3 (no additives in feed), EG1-3 show that the present invention can effectively reduce the prevalence of hoof and limb diseases in pigs; compared with CG 1, the present invention has a slightly better preventive effect on hoof and limb diseases than the commercially available product "Super Vita"; compared with CG 2, it shows that the fermented Chinese medicine composition design of the present invention can effectively improve the preventive effect on hoof and limb diseases, which is the key design of the present invention.

[0253] (3) 20 farmed pigs with early symptoms of limb and hoof disease, specifically redness, swelling or slight erosion of the limbs and hooves, accompanied by loss of appetite and / or mild diarrhea (i.e., generally indicating bacterial limb and hoof disease), were randomly divided into two groups, 10 in each group, group numbers SP-EG1 and SP-CG1, and Example 2 and Comparative Example 2 were added to the feed, respectively, at a ratio of 2 kg per ton of feed.

[0254] The trial lasted two weeks, during which time the pigs were observed for improvement or recovery. All pigs were fed three times daily with ad libitum access to feed and water. The breeding area was maintained clean, sanitary, and well-ventilated.

[0255] Group SP-EG1 SP-CG1 Number of animals with improved limb and hoof symptoms 10 6

[0256] The data of Comparative Example 2 show that the scientific and reasonable configuration of nutrients such as vitamins can improve the symptoms of limb and hoof disease in pigs by enhancing the immunity of pigs. However, the improvement of limb and hoof disease in pigs by adding the feed of Comparative Example 2 is not as good as that by adding the feed of Example 2. This shows that the fermented Chinese medicine composition designed by the present invention can effectively improve the therapeutic effect of the additive on mild limb and hoof disease.

[0257] Based on the disclosure and teachings of the above description, those skilled in the art may also make changes and modifications to the above embodiments. Therefore, the present invention is not limited to the specific embodiments disclosed and described above, and some modifications and variations of the present invention should also fall within the scope of protection of the claims of the present invention. In addition, although some specific terms are used in this description, these terms are only for convenience of description and do not constitute any limitation to the present invention.

Claims

1. An additive for preventing and treating pig limb and hoof diseases, characterized in that: The preparation raw materials at least include composite bacterial fermentation liquid and traditional Chinese medicine composition; The composite bacterial fermentation liquid comprises Bacillus subtilis fermentation liquid, Lactobacillus lactis fermentation liquid and Saccharomyces cerevisiae fermentation liquid in a weight ratio of 0.8-1.0:1.0-1.2:0.8-1.0; The Chinese medicine composition is composed of the following raw materials in parts by weight: 55-70 parts of psoralea corylifolia, 45-53 parts of atractylodes macrocephala, 45-53 parts of poria cocos, 35-43 parts of frankincense, 35-43 parts of turmeric, 35-43 parts of raw rehmannia root, 20-33 parts of dipsaci, 20-33 parts of orientalis, 20-33 parts of white peony root, 20-33 parts of myrrh and 20-33 parts of liquorice; When preparing the additive, the fermentation raw materials at least include the following components in parts by weight: 100 parts of crushed traditional Chinese medicine composition, 8-12 parts of composite bacterial fermentation liquid, 20-25 parts of wheat bran, 5-10 parts of soybean meal, 2.5-8.0 parts of calcium carbonate, 0.4-0.6 parts of tryptophan, 0.1-0.5 parts of ferrous sulfate, 0.1-0.3 parts of zinc sulfate, 0.05-0.1 parts of manganese sulfate or acid manganese phosphate, 0.05-0.1 parts of magnesium sulfate, 0.05-0.1 parts of copper sulfate and 0.01-0.05 parts of disodium hydrogen phosphate; the preparation method of the additive at least includes: adding water to the fermentation raw materials to adjust the water content to 50-60%, adjusting the pH value to 5.5-7.0, fermenting at 25-30°C, and fermenting for 96-120 hours; turning and stirring at least twice every 24 hours, each time for 8-10 minutes, and when the total bacterial count reaches ≥1.0×10 7 Fermentation was stopped when the CFU / ml was 3≤pH≤4.

2. The preparation method of the additive for preventing and treating pig limb and hoof disease according to claim 1, characterized in that: The steps include: 1) reviving and culturing Bacillus subtilis, Lactobacillus lactis and Saccharomyces cerevisiae to obtain Bacillus subtilis fermentation broth, Lactobacillus lactis fermentation broth and Saccharomyces cerevisiae fermentation broth; 2) mixing Bacillus subtilis fermentation broth, Lactobacillus lactis fermentation broth and Saccharomyces cerevisiae fermentation broth in a preset weight ratio to produce a composite bacterial fermentation broth; 3) preparing the Chinese medicine composition according to the preset components and weight proportions, and crushing the mixture; 4) preparing fermentation raw materials and performing fermentation; 5) After the fermentation is completed, the product is bottled to obtain an additive for preventing and treating pig limb and hoof diseases.

3. A pig feed, characterized in that ， The additive for preventing and treating pig limb and hoof diseases according to claim 1 is added at a ratio of 1 to 3 kg of the additive per ton of pig feed.

4. Use of the additive for preventing and treating pig limb and hoof diseases according to claim 1 in preparing feed for preventing and treating nutritional limb and hoof diseases and bacterial limb and hoof diseases.

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