Chromatography device with backwashing function

Abstract

The present invention discloses a chromatography device with a backwashing function, which relates to the technical field of chromatography devices, and aims to solve the technical problem of the simple overall structure of the chromatography device with a flushing function, including a guide rail; the chromatography component is arranged on the guide rail through a support frame, wherein the internal gap of the chromatography component constitutes a chromatography channel, and both ends of the chromatography channel are annularly provided with a number of connecting gaps at equal intervals; the connecting gaps are all sealed by a one-way sealing component. The present invention adapts to high-concentration chromatography detection objects through a backwashing operation, although the adsorption equilibrium of the solid phase will be broken, the flushing and cleaning effect is good, and adapts to low-concentration chromatography detection object operation through a forward flushing operation, which can meet the basic flushing effect and reduce the occurrence of adsorption equilibrium breaking of the solid phase. The above two operation modes can effectively carry out chromatography detection objects of different concentrations, effectively improving the operational functionality.

Description

Technical Field

[0001] The invention relates to the technical field of chromatography devices, and more specifically to a chromatography device with a backwashing function. Background Art

[0002] Chromatography is a highly efficient chemical separation tool designed based on the principle of chemical affinity differences. It is widely used in medicine, life sciences, chemistry, environmental protection and other fields. The following is a detailed introduction to chromatography:

[0003] Stationary phase: An important component in chromatographic separation, it refers to a compound coated on a carrier in a column. Its function is to adsorb or react the compounds in the mixture to be separated according to the affinity between them and the stationary phase. Common stationary phases include silica gel, dextran, polyvinyl alcohol, etc. Mobile phase: Also known as mobile phase, it refers to a solvent or solution, a liquid that contacts the stationary phase in the column and gradually flows downward. It gradually brings out the mixture adsorbed or reacted on the stationary phase by continuously flowing downward, and separates them according to the affinity between them and the mobile phase. Common mobile phases include water, methanol, chloroform, etc. Column: One of the most important components of a chromatographic device, made of a tube with a stationary phase coated on the inner wall. According to different experimental requirements, the column can be divided into an adsorption column, an ion exchange column, a gel filtration column, etc. Its function is to gradually separate the mixture to be separated through the stationary phase layer. Matching detector: It is used to detect and record various compounds flowing out of the column, and to perform quantitative or qualitative analysis on these compounds. Common detectors include ultraviolet detectors, fluorescence detectors, conductivity detectors, etc.

[0004] The technical principles of chromatography devices are mainly based on molecular sieving, ion exchange and affinity chromatography. The molecular sieving effect is due to the pore size of a certain specification on the filler. Samples with different molecular weights are subjected to different forces when flowing through the column, and the paths they take are also different. Therefore, the time it takes to flow out of the column is different, so that different components can be separated. Ion exchange is due to the attraction of positive and negative charges, which causes the sample to hang on the filler, changing the solution environment, causing the charge carried by the sample molecules to change, and thus being eluted. Different components have different adsorption forces with the column material, so the elution conditions are also different. Affinity chromatography combines the column material with the specific adsorption of the separated sample to separate the separated substance from other components.

[0005] The existing chromatography device or adapter is equipped with a structure or device to assist in flushing to perform flushing operation on the chromatography device after the chromatography operation is completed. The most critical flushing is the adsorption column, ion exchange column, gel filtration column and the chromatographic vessel for the chromatography. The existing flushing device is mostly a single one-way flushing operation. During the flushing process, the internal structural characteristic pores of the adsorption column, ion exchange column and gel filtration column will more or less produce certain adsorbents remaining in the pores, which leads to the inaccuracy of the flushing operation and the limitations of the existing one-way flushing operation function;

[0006] During a single forward flush: Follow the normal flow direction of the mobile phase: The main reasons why the forward flush operation is not likely to cause packing loss or damage the column bed structure are as follows: Flow direction consistency: During forward flushing, the flow direction of the flushing liquid is the same as the mobile phase direction during normal operation. This consistency ensures that the pressure and shear force on the packing particles during the flushing process are relatively small, thereby reducing the risk of packing loss. Tightly arranged packing: In chromatography columns, the packing is usually tightly arranged, which helps to maintain the stability of the packing during forward flushing. Due to the interaction and support between the packing, the packing particles are not easy to move out of their position even if they are flushed by a certain amount of fluid. Column packing pressure: When packing the column, a certain amount of pressure is usually applied to ensure that the packing is tightly packed. This pressure forms a relatively stable structure between the packing particles, which can resist a certain degree of fluid flushing. During the forward flushing process, this stable structure is maintained, thereby reducing the risk of packing loss. This operation mode is suitable for low-concentration chromatographic detection objects.

[0007] In a single backwash: in the opposite direction of the mobile phase: During backwashing, the flushing liquid enters from the other end of the column and flows through the packing layer in the opposite direction of the normal flow direction. This reverse flow can break the adsorption state of pollutants and impurities that are tightly bound to the packing. Remove pollutants and impurities that are tightly bound to the packing: Since backwashing can break the adsorption balance between pollutants and impurities and the packing, these difficult-to-remove substances can be desorbed from the packing to achieve a deeper level of cleaning. Achieve deep cleaning: Backwashing can flush every corner of the packing layer, including areas that are difficult to reach during forward flushing, so a more comprehensive and deeper cleaning effect can be achieved. This operation mode is suitable for high-concentration chromatographic detection objects.

[0008] The existing chromatography device with flushing function has a simple overall structure and a single function, and cannot effectively adapt to the flushing operation after the detection of chromatographic test objects with different concentrations. Therefore, it is particularly important to propose a chromatography device with rich functionality and efficient flushing and cleaning operation. In view of this, we propose a chromatography device with backwashing function. Summary of the invention

[0009] The object of the present invention is to provide a chromatography device with a backwashing function, so as to solve the technical problem of a simple overall structure of the chromatography device with a flushing function.

[0010] In order to solve the above technical problems, the present invention provides the following technical solutions: a chromatography device with backwashing function, comprising a guide rail; the chromatography component is arranged on the guide rail through a support frame, wherein the internal gap of the chromatography component constitutes a chromatography channel, and both ends of the chromatography channel are provided with a plurality of connecting gaps in a circular shape and at equal intervals; the connecting gaps are all sealed by a one-way sealing component; wherein the connecting gap arranged relatively close to the input end of the chromatography channel is a forward flushing connection channel; and the connecting gap arranged relatively close to the output end of the chromatography channel is a reverse flushing connection channel ; The flushing system is slidably arranged outside the chromatography component and connected to the movable end of the guide rail; the flushing system includes a basic connecting ring frame slidably arranged outside the chromatography component; one side of the basic connecting ring frame is hinged with a plurality of connecting arms in a circular shape with equal spacing; the inner wall gaps of the plurality of connecting arms arranged in a circular shape with equal spacing constitute a rotation adjustment cavity; a driving disk is rotatably arranged inside the rotation adjustment cavity; the connecting arm is hinged with a fitting adjustment unit; wherein, any one of the fitting adjustment units is provided with a flushing connector connected to an external water purification pump to provide equipment; wherein, a plurality of connecting arms arranged in a circular shape with equal spacing constitute a rotation adjustment cavity; a driving disk is rotatably arranged inside the rotation adjustment cavity; the connecting arm is hinged with a fitting adjustment unit; wherein, a flushing connector connected to an external water purification pump to provide equipment is provided on the surface of the connecting arm; wherein, a plurality of connecting arms arranged in a circular shape with equal spacing constitute a rotation adjustment cavity; The fitting adjustment units distributed at a certain distance are connected by an articulated telescopic tube assembly; wherein, a plurality of the fitting adjustment units are connected to the inside of the articulated telescopic tube assembly to form a single-channel inner shaft perfusion and flushing structure; and the flushing connector is connected to the inner channel of the fitting adjustment unit; wherein, the single-channel inner shaft perfusion and flushing structure has an extrusion-type connection state and a tangential-type loose state; in the tangential-type loose state of the single-channel inner shaft perfusion and flushing structure, when the guide rail drives the flushing system to slide close to the chromatographic channel input end of the communication gap to the forward flushing connection channel, the single-channel The inner shaft perfusion and flushing structure is adjusted to an extrusion-type connected state, so that the single-channel inner shaft perfusion and flushing structure is connected to the chromatography component to form a forward flushing structure for flushing the stationary phase inside the chromatography component; when the guide rail drives the flushing system to slide toward the chromatography channel output end of the connecting notch to the reverse flushing connection channel, the single-channel inner shaft perfusion and flushing structure is adjusted to an extrusion-type connected state, so that the single-channel inner shaft perfusion and flushing structure is connected to the chromatography component to form a reverse flushing structure for flushing the stationary phase inside the chromatography component.

[0011] The present invention is based on a single-channel inner shaft perfusion flushing structure to perform an extrusion-type connection state and a tangential loose state and switch adjustment, so that the flushing output end of the single-channel inner shaft perfusion flushing structure is connected with the connecting gap and away from the blockage, and cooperates with the guide rail sliding drive adjustment so that the flushing system as a whole can be freely selected to be adjusted to the forward flushing connection channel and the reverse flushing connection channel, and cooperates with the channel diversion adjustment of the diverter valve. It has reverse flushing and forward flushing operations while maintaining basic chromatography operations. The reverse flushing operation is used to adapt to high-concentration chromatography detection objects. Although the adsorption equilibrium of the solid phase will be disrupted, the flushing and cleaning effect is good. The forward flushing operation is used to adapt to low-concentration chromatography detection object operations, which can meet the basic flushing effect and reduce the occurrence of solid phase adsorption equilibrium disruption. The above two operation modes can effectively carry out chromatography detection objects of different concentrations, effectively improving the operational functionality.

[0012] Preferably, the two support frames are symmetrically arranged on the guide rail, and the two support frames are connected by at least one auxiliary connecting shaft.

[0013] Preferably, the chromatography component comprises a chromatography receiving pipe arranged on the support frame, and both ends of the chromatography receiving pipe are connected to the external processing pipe through a diverter valve; hollow baffle plates are symmetrically arranged on both axial sides inside the chromatography receiving pipe between the forward flushing connecting channel and the reverse flushing connecting channel, wherein a stationary phase is filled between the two baffle plates.

[0014] Preferably, the one-way sealing assembly includes a three-way sealing block arranged inside the connecting gap through a spring; wherein a "Y"-shaped connecting port is provided inside the three-way sealing block; the cross-section of the three-way sealing block is a trapezoidal structure; wherein the cross-section of the connecting gap is spindle-shaped, and an auxiliary matching gap with a larger outside and a smaller inside is provided relatively close to the outside of the chromatography receiving pipe; and the connecting gap is relatively close to one side of the inclined surface of the three-way sealing block and is matched with the inclination angle of the inclined surface of the three-way sealing block.

[0015] Preferably, the basic connecting ring frame is slidably arranged on the auxiliary connecting shaft, and the basic connecting ring frame and the chromatography receiving pipe are coaxially arranged; and an extension convex shaft is arranged on the side of the basic connecting ring frame, and a push rod is hingedly arranged on the extension convex shaft; wherein, a plurality of hinge shafts are arranged in a circular shape at equal intervals on the surface of the basic connecting ring frame, and a "convex"-shaped limit block is rotatably arranged on the hinge shaft; the connecting arm is hingedly arranged on the hinge shaft, and the relative gaps between a plurality of the connecting arms and a plurality of hinge shafts constitute a rotation adjustment chamber.

[0016] Preferably, a plurality of inclined extrusion grooves are arranged in an annular shape at equal intervals on the surface of the driving disk, and the side extension end of the driving disk is hingedly connected to the telescopic end of the push rod.

[0017] Preferably, the fitting adjustment unit includes a connecting block; the connecting block is hingedly arranged at the end of the connecting arm, wherein an extrusion pin that slides with the extrusion groove is provided on the surface of the connecting block; and, the connecting block is relatively close to the chromatography receiving pipe and is provided with an arc surface portion and a straight line portion in sequence, and the arc surface portion and the straight line portion are tangently distributed, wherein an extrusion connecting protrusion is provided on the outer wall of the connecting block relatively close to the chromatography receiving pipe; wherein a hinge protrusion and a hinge accommodating groove are respectively provided on the side of the connecting block relatively close to the adjacent connecting block; wherein, an internal connecting channel that is connected to the hinge protrusion, the hinge accommodating groove and the extrusion connecting protrusion is provided inside the connecting block.

[0018] Preferably, the articulated telescopic tube assembly includes a hollow rotating sleeve A hingedly arranged on the hinged protrusion; a hollow rotating sleeve B is provided on one side of the hollow rotating sleeve A through a telescopic tube, and the hollow rotating sleeve B is rotatably connected to the hinged accommodating groove.

[0019] Preferably, the hollow rotating sleeve A and the hollow rotating sleeve B are both provided with a conveying hole on the outside, the size of which is smaller than the size of the internal connecting channel.

[0020] A method for using a chromatography device with a backwashing function comprises the following steps:

[0021] Installation and processing: connect the two axial ends of the chromatography receiving pipeline in the chromatography device to the detection object delivery end and the sewage receiving tank through the diverter valve, and connect the other end to the detection object processing receiving vessel and the sewage receiving tank through the diverter valve; and connect the water purification tank pump delivery end to the flushing connector through the pipeline;

[0022] S2, chromatography treatment: by opening the detection object delivery end on one side of the chromatography receiving pipeline input end and opening the detection object processing receiving vessel at the chromatography receiving pipeline output end, the chromatography detection is chromatographed through the solid phase;

[0023] S3, flushing pretreatment: by closing the test object delivery end on one side of the chromatography receiving pipeline input end and closing the test object processing receiving vessel at the chromatography receiving pipeline output end;

[0024] S4, flushing and adjustment process:

[0025] S4.1. If positive flushing adjustment is performed after chromatography on low concentration test substances:

[0026] First, the basic connection ring frame is driven to rotate by the push rod return work, and the extrusion pin is rotated and offset by the inclined extrusion groove, so that the connection block is adjusted synchronously, so that the connection block is unfolded, and then the flushing system is driven to slide as a whole by the guide rail, so that the connection block moves relatively close to the forward flushing connection channel, and then the basic connection ring frame is driven to rotate by the push rod stroke work, and the extrusion pin is rotated and offset by the inclined extrusion groove, so that the connection block is adjusted synchronously, so that the connection block shrinks, and at the same time, the hollow rotating sleeve A and the hollow rotating sleeve B move close, and the telescopic tube moves in compression, and then the extrusion connecting protrusion is squeezed on the three-way blocking block based on the rotation offset adjustment, so that the connecting gap is connected, and then the diverter valve of the detection object conveying end and the sewage receiving tank is closed, and the diverter valve of the detection object processing receiving vessel at the other end is opened;

[0027] S4.2. If backwashing and adjustment are performed after chromatography on high-concentration test substances:

[0028] First, the basic connection ring frame is driven to rotate by the push rod return work, and the extrusion pin is rotated and offset by the inclined extrusion groove, so that the connection block is adjusted synchronously, so that the connection block is unfolded, and then the flushing system is driven to slide as a whole by the guide rail, so that the connection block moves relatively close to the reverse flushing connection channel, and then the basic connection ring frame is driven to rotate by the push rod stroke work, and the extrusion pin is rotated and offset by the inclined extrusion groove, so that the connection block is adjusted synchronously, so that the connection block shrinks, and at the same time, the hollow rotating sleeve A and the hollow rotating sleeve B move close, and the telescopic tube moves in compression, and then the extrusion connecting protrusion is squeezed on the three-way blocking block based on the rotation offset adjustment, so that the connecting gap is connected, and then the diverter valves of the detection object processing receiving vessel and the sewage receiving tank at the output end are closed, and the diverter valves of the detection object conveying end and the sewage receiving tank are opened;

[0029] S5. Flushing treatment: The clean water is pumped into the flushing connector through the clean water tank pump, and then diverted through the internal connecting channel of the connecting block and poured into the connected hinged telescopic pipe assembly to form a circular flow, and finally flows from the extruded connecting protrusion into the connecting notch to flush the solid phase into the sewage receiving tank.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] 1. The present invention is based on a single-channel inner shaft perfusion flushing structure to perform an extrusion-type connection state and a tangential loose state and switch adjustment, so that the flushing output end of the single-channel inner shaft perfusion flushing structure is connected with the connection gap and away from the blockage, and cooperates with the guide rail sliding drive adjustment so that the flushing system as a whole can be freely selected to be adjusted to the forward flushing connection channel and the reverse flushing connection channel, and cooperates with the channel diversion adjustment of the diverter valve. It has reverse flushing and forward flushing operations while maintaining basic chromatography operations. The reverse flushing operation is adapted to high-concentration chromatography detection objects. Although the adsorption equilibrium of the solid phase will be disrupted, the flushing and cleaning effect is good. The forward flushing operation is adapted to low-concentration chromatography detection object operation, which can meet the basic flushing effect and reduce the occurrence of solid phase adsorption equilibrium disruption. The above two operation modes can effectively carry out chromatography detection objects of different concentrations, effectively improving the operational functionality.

[0032] 2. The present invention linearly stabilizes the flushing system by setting an auxiliary connecting shaft, thereby preventing the basic connecting ring frame from following the movement, which would cause a change in the size distance between the flushing system and the connecting gap.

[0033] 3. The present invention limits the fixed phase by means of a hollow blocking plate, so that the limited solid phase can always be fully contacted during the forward flushing or reverse flushing operation, thereby improving the flushing effect.

[0034] 4. The present invention applies elastic force to the three-way sealing block through a spring, so that the three-way sealing block can use the inclined surface of the three-way sealing block to closely fit the connecting gap to form a sealing effect without the action of external pressure; at the same time, an auxiliary adapter gap is set to meet the space size required when the protrusion at the output end of the flushing system squeezes the three-way sealing block. This setting is used to realize the pressing and connecting operation of the three-way sealing block while avoiding the unstable movement of the protrusion at the output end of the flushing system contacting the connecting gap.

[0035] 5. The present invention can drive the driving disk to perform forward and reverse rotation operations through the push rod's return operation to cause several connecting blocks to rotate and dislocate, so that the extruded connecting protrusions are close to or away from the connecting notches to achieve the filling and flushing operations of clean water, as well as the forward and reverse flushing operations during the operation, and basic chromatography operations.

[0036] 6. The present invention is based on the fitting movement characteristics of the side of the connecting block in the fitting adjustment unit, so that the hinged telescopic tube assembly as a whole performs embedded telescopic adjustment, and the hinged telescopic tube assembly is adjusted to adapt to the filling and flushing operation of purified water and the forward and reverse flushing operation in the operation.

[0037] 7. The present invention provides a delivery hole with a size smaller than that of the internal connecting channel on the outside of the hollow rotating sleeve A and the hollow rotating sleeve B, so that the angular deviation of the hollow rotating sleeve A and the hollow rotating sleeve B during the close matching and away operations of the filling and flushing of purified water can be compensated, thereby avoiding the misalignment of the internal connecting channel due to the synchronous adjustment of the rotating delivery hole. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0039] Figure 2 It is a schematic diagram of the side structure of the flushing system of the present invention;

[0040] Figure 3 It is a schematic diagram of the cross-sectional three-dimensional structure of the chromatography component of the present invention;

[0041] Figure 4 It is a schematic diagram of the three-dimensional structure of the one-way sealing assembly of the present invention;

[0042] Figure 5 For the present invention Figure 1 A schematic diagram of the partially enlarged structure at center A;

[0043] Figure 6 It is a schematic diagram of the side view structure of the drive disk of the present invention;

[0044] Figure 7 It is a schematic diagram of the cross-sectional three-dimensional structure of the fitting adjustment unit of the present invention;

[0045] Figure 8 For the present invention Figure 7 A schematic diagram of the partially enlarged structure at B in the middle;

[0046] Fig. 9 It is a schematic diagram of the installation structure of the fitting adjustment unit and the articulated telescopic tube assembly of the present invention;

[0047] Fig.10 It is a side view structural diagram of the bonding adjustment unit and the chromatography component in the present invention to illustrate the bonding state of the chromatography components.

[0048] Description of the numbers in the figure:

[0049] 1. Guide rail; 2. Chromatography assembly; 3. Support frame; 4. Flushing system; 5. Connecting gap; 6. One-way sealing assembly; 7. Basic connecting ring frame; 8. Connecting arm; 9. Driving disc; 10. Fitting adjustment unit; 11. Articulated telescopic tube assembly;

[0050] 201, chromatography receiving pipeline; 202, blocking plate;

[0051] 501, auxiliary adaptation gap;

[0052] 601, three-way blocking block; 6011, connecting port; 602, spring;

[0053] 701, hinge shaft; 702, limit block; 703, push rod;

[0054] 901, extrusion tank;

[0055] 1001, connecting block; 1002, arc surface; 1003, straight portion; 1004, extrusion connection protrusion; 1005, hinged protrusion; 1006, hinged receiving groove; 1007, internal communication channel;

[0056] 1101. Hollow rotating sleeve A; 1102. Telescopic tube; 1103. Hollow rotating sleeve B. DETAILED DESCRIPTION

[0057] like Figures 1 to 10As shown, the present invention relates to a chromatography device with backwashing function, comprising a guide rail 1; a chromatography component 2 is arranged on the guide rail 1 through a support frame 3, wherein the internal gap of the chromatography component 2 constitutes a chromatography channel, and a plurality of connecting gaps 5 are arranged at equal intervals in a circular shape at both ends of the chromatography channel; the connecting gaps 5 are all sealed by a one-way sealing component 6; wherein the connecting gap 5 arranged relatively close to the input end of the chromatography channel is a forward flushing connection channel; and the connecting gap 5 arranged relatively close to the output end of the chromatography channel is a reverse flushing connection channel; the flushing system 4 is arranged slidingly The movable end of the guide rail 1 is connected to the outside of the chromatography component 2; the flushing system 4 includes a basic connecting ring frame 7 slidably arranged outside the chromatography component 2; a plurality of connecting arms 8 are hingedly connected in a circular shape with equal spacing on one side of the basic connecting ring frame 7; the inner wall gaps of the plurality of connecting arms 8 arranged in a circular shape with equal spacing constitute a rotation adjustment cavity; a driving disk 9 is rotatably arranged inside the rotation adjustment cavity; a fitting adjustment unit 10 is hingedly arranged on the connecting arm 8; wherein, a flushing connector connected to an external clean water pump to the device is arranged on the surface of any fitting adjustment unit 10; wherein, a plurality of fitting adjustment units 10 are distributed in a circular shape with equal spacing. The sections 10 are connected by an articulated telescopic tube assembly 11; wherein, a plurality of fitting adjustment units 10 are connected to the inside of the articulated telescopic tube assembly 11 to form a single-channel inner shaft perfusion and flushing structure; and, the flushing connector is connected to the inner channel of the fitting adjustment unit 10; wherein, the single-channel inner shaft perfusion and flushing structure has an extrusion-type connection state and a tangential-type loose state; in the tangential-type loose state of the single-channel inner shaft perfusion and flushing structure, when the guide rail 1 drives the flushing system 4 to slide toward the chromatographic channel input end of the connecting notch 5 to the forward flushing connection channel, the single-channel inner shaft perfusion and flushing The flushing structure is adjusted to an extrusion-type connected state, so that the single-channel inner-axis perfusion flushing structure is connected to the chromatography component 2 to form a forward flushing structure for flushing the stationary phase inside the chromatography component 2; when the single-channel inner-axis perfusion flushing structure is in a tangentially loosened state, the guide rail 1 drives the flushing system 4 to slide toward the chromatography channel output end of the connecting notch 5 to the reverse flushing connection channel, and the single-channel inner-axis perfusion flushing structure is adjusted to an extrusion-type connected state, so that the single-channel inner-axis perfusion flushing structure is connected to the chromatography component 2 to form a reverse flushing structure for flushing the stationary phase inside the chromatography component 2.The present invention is based on a single-channel inner shaft perfusion flushing structure to perform an extrusion-type connection state and a tangential loose state and switch adjustment, so that the flushing output end of the single-channel inner shaft perfusion flushing structure is connected with the connecting gap 5 and away from the blockage, and cooperates with the sliding drive adjustment of the guide rail 1 so that the flushing system 4 as a whole can be freely selected to adjust to the forward flushing connection channel and the reverse flushing connection channel, and cooperates with the channel diversion adjustment of the diverter valve. While maintaining the basic chromatography operation, it has reverse flushing and forward flushing operations. The reverse flushing operation is used to adapt to high-concentration chromatography detection objects. Although the adsorption equilibrium of the solid phase will be broken, the flushing and cleaning effect is good. The forward flushing operation is used to adapt to the operation of low-concentration chromatography detection objects, which can meet the basic flushing effect and reduce the occurrence of solid phase adsorption equilibrium breaking. The above two operation modes can effectively carry out chromatography detection objects of different concentrations, effectively improving the operational functionality.

[0058] In the embodiment of the present invention, two support frames 3 are symmetrically arranged on the guide rail 1, and the two support frames 3 are connected by at least one auxiliary connecting shaft. The present invention linearly stabilizes the flushing system 4 by setting the auxiliary connecting shaft, and avoids the basic connecting ring frame 7 from following the movement, which causes the size distance between the flushing system 4 and the connecting gap 5 to change.

[0059] In the embodiment of the present invention, the chromatography assembly 2 includes a chromatography receiving pipe 201 arranged on the support frame 3, and both ends of the chromatography receiving pipe 201 are connected to the external processing pipe through a diverter valve; hollow blocking plates 202 are symmetrically arranged on both axial sides of the inside of the chromatography receiving pipe 201, relatively located between the forward flushing connection channel and the reverse flushing connection channel, wherein a stationary phase is filled and arranged between the two blocking plates 202. The present invention limits the stationary phase by the hollow blocking plate 202, so that the limited solid phase can always be fully contacted during the forward flushing or reverse flushing operation, thereby improving the flushing effect.

[0060] In an embodiment of the present invention, the one-way sealing component 6 includes a three-way sealing block 601 arranged inside the connecting gap 5 through a spring 602; wherein, a "Y"-shaped connecting port 6011 is provided inside the three-way sealing block 601; the cross-section of the three-way sealing block 601 is a trapezoidal structure; wherein, the cross-section of the connecting gap 5 is spindle-shaped, and, an auxiliary adaption gap 501 with a larger outside and a smaller inside is provided relatively close to the outside of the chromatography receiving pipe 201; and, the connecting gap 5 is relatively close to the inclined surface of the three-way sealing block 601 on one side and is adapted to the inclination angle of the inclined surface of the three-way sealing block 601. The present invention uses a spring 602 to apply an elastic force to the three-way sealing block 601, so that the three-way sealing block 601 uses the inclined surface of the three-way sealing block 601 to tightly fit the connecting gap 5 to form a sealing effect in the absence of external pressure; at the same time, the auxiliary adapter gap 501 is set to the required space size when the protrusion at the output end of the flushing system 4 squeezes the three-way sealing block 601. This setting is used to realize the pressing and connecting operation of the three-way sealing block 601 while avoiding the unstable movement of the protrusion at the output end of the flushing system 4 contacting the connecting gap 5.

[0061] In an embodiment of the present invention, the basic connecting ring frame 7 is slidably arranged on the auxiliary connecting shaft, and the basic connecting ring frame 7 and the chromatography receiving pipe 201 are coaxially arranged; and an extension convex shaft is arranged on the side of the basic connecting ring frame 7, and a push rod 703 is hingedly arranged on the extension convex shaft; wherein, a plurality of hinge shafts 701 are arranged in a circular shape with equal intervals on the surface of the basic connecting ring frame 7, and a "convex"-shaped limit block 702 is rotatably arranged on the hinge shaft 701; the connecting arm 8 is hingedly arranged on the hinge shaft 701, and the relative gap between a plurality of connecting arms 8 and a plurality of hinge shafts 701 constitutes a rotation adjustment cavity.

[0062] In the embodiment of the present invention, a plurality of inclined extrusion grooves 901 are arranged in a ring shape at equal intervals on the surface of the driving disk 9 , and the side extension end of the driving disk 9 is hingedly connected to the telescopic end of the push rod 703 .

[0063] In an embodiment of the present invention, the fitting adjustment unit 10 includes a connecting block 1001; the connecting block 1001 is hingedly arranged at the end of the connecting arm 8, wherein the surface of the connecting block 1001 is provided with an extrusion pin that slides with the extrusion groove 901; and, the connecting block 1001 is relatively close to the side of the chromatography receiving pipe 201 and is sequentially provided with an arc portion 1002 and a straight portion 1003, and the arc portion 1002 and the straight portion 1003 are tangentially distributed, wherein the outer wall of the connecting block 1001 is relatively close to the chromatography receiving pipe 201 and is provided with an extrusion connecting protrusion 1004; wherein, the side of the connecting block 1001 is relatively close to the adjacent connecting block 1001 and is respectively provided with a hinge protrusion 1005 and a hinge accommodating groove 1006; wherein, the interior of the connecting block 1001 is provided with an internal connecting channel 1007 that is connected to the hinge protrusion 1005, the hinge accommodating groove 1006 and the extrusion connecting protrusion 1004. The present invention can drive the driving disk 9 to perform forward and reverse rotation operations through the return operation of the push rod 703 to cause the multiple connecting blocks 1001 to rotate and dislocate, so that the extruded connecting protrusion 1004 is close to or away from the connecting notch 5 to achieve the filling and flushing operation of clean water, the approach and distance, the forward and reverse flushing operation during the operation, and the basic chromatography operation.

[0064] In the embodiment of the present invention, the articulated telescopic tube assembly 11 includes a hollow rotating sleeve A1101 hingedly arranged on the hinge protrusion 1005; a hollow rotating sleeve B1103 is provided on one side of the hollow rotating sleeve A1101 through the telescopic tube 1102, and the hollow rotating sleeve B1103 is rotatably connected to the hinged receiving groove 1006. Based on the fitting movement characteristics of the side of the connecting block 1001 in the fitting adjustment unit 10, the present invention enables the articulated telescopic tube assembly 11 to be integrally telescopically adjusted in an embedded manner, and the articulated telescopic tube assembly 11 is telescopically adjusted to adapt to the filling and flushing operation of purified water and the forward and reverse flushing operation in the operation.

[0065] In the embodiment of the present invention, the hollow rotating sleeve A1101 and the hollow rotating sleeve B1103 are both provided with a delivery hole whose size is smaller than the size of the internal connecting channel 1007. The present invention compensates for the angular deviation of the hollow rotating sleeve A1101 and the hollow rotating sleeve B1103 during the close matching and far away operation of the filling and flushing operation of the purified water, thereby avoiding the misalignment of the internal connecting channel 1007 due to the synchronous adjustment of the rotating delivery hole.

[0066] Working principle: This embodiment provides a chromatography device with backwashing function, comprising the following steps:

[0067] S1. Installation: The two axial ends of the chromatography receiving pipe 201 in the chromatography device are connected to the detection object delivery end and the sewage receiving tank through the diverter valve, and the other end is connected to the detection object processing receiving vessel and the sewage receiving tank through the diverter valve; and the pump delivery end of the clean water tank is connected to the flushing connector through the pipe;

[0068] S2, chromatography treatment: by opening the detection object delivery end on one side of the input end of the chromatography receiving pipeline 201 and opening the detection object processing receiving vessel at the output end of the chromatography receiving pipeline 201, the chromatography detection is chromatographed through the solid phase;

[0069] S3, flushing pretreatment: by closing the detection object conveying end on one side of the input end of the chromatography receiving pipe 201 and closing the detection object processing receiving vessel at the output end of the chromatography receiving pipe 201;

[0070] S4, flushing and adjustment process:

[0071] S4.1. If positive flushing adjustment is performed after chromatography on low concentration test substances:

[0072] First, the push rod 703 is used to drive the basic connection ring frame 7 to rotate, and the inclined extrusion groove 901 is used to make the extrusion pin rotate and deviate, so that the connection block 1001 is adjusted synchronously, so that the connection block 1001 is unfolded, and then the flushing system 4 is driven to slide as a whole through the guide rail 1, so that the connection block 1001 moves relatively close to the forward flushing connection channel, and then ... unfolded, and then the flushing system 4 is driven to slide as a whole through the guide rail 1, so that the connection block 1001 moves relatively close to the forward flushing connection channel, and then the push rod 703 is used to drive the basic connection ring frame 7 to rotate, and the inclined extrusion groove 901 is used to make the extrusion pin rotate and deviate, so that the connection block 1001 is unfolded, and then the flushing system 4 is driven to slide as a whole through the guide rail 1, so that the connection block 1001 moves relatively close to the forward flushing connection channel, and then the push rod 703 is used to drive the basic connection ring frame 7 to rotate, and the inclined extrusion groove 901 is used to make the extrusion pin rotate and deviate, so that the connection block 10 The pressing pin performs a rotational offset movement, causing the connecting block 1001 to be adjusted synchronously, so that the connecting block 1001 contracts, and at the same time the hollow rotating sleeve A1101 and the hollow rotating sleeve B1103 move closer, and the telescopic tube 1102 compresses and moves, and then the extrusion connecting protrusion 1004 squeezes the three-way blocking block 601 based on the rotational offset adjustment, so that the connecting gap 5 is connected, and then the diverter valves of the detection object conveying end and the sewage receiving tank are closed, and the diverter valve of the detection object processing receiving vessel at the other end is opened;

[0073] S4.2. If backwashing and adjustment are performed after chromatography on high-concentration test substances:

[0074] First, the push rod 703 is used to drive the basic connection ring frame 7 to rotate by the return work, and the extrusion pin is rotated and offset by the inclined extrusion groove 901, so that the connection block 1001 is adjusted synchronously, so that the connection block 1001 is unfolded, and then the flushing system 4 is driven to slide as a whole by the guide rail 1, so that the connection block 1001 moves relatively close to the reverse flushing connection channel, and then the basic connection ring frame 7 is driven to rotate by the stroke work of the push rod 703, and the extrusion pin is rotated by the inclined extrusion groove 901. The rotational offset movement causes the connection block 1001 to be adjusted synchronously, so that the connection block 1001 shrinks, and at the same time the hollow rotating sleeve A 1101 and the hollow rotating sleeve B 1103 move closer, and the telescopic tube 1102 moves in compression, and then the extrusion connection protrusion 1004 squeezes the three-way blocking block 601 based on the rotational offset adjustment, so that the communication gap 5 is connected, and then the diverter valves of the output end detection object processing receiving vessel and the sewage receiving tank are closed, and the diverter valves of the detection object delivery end and the sewage receiving tank are opened;

[0075] S5. Flushing treatment: clean water is poured into the flushing connector through the clean water tank pump, and is diverted through the internal connecting channel 1007 of the connecting block 1001 and poured into the connected articulated telescopic tube assembly 11 to form a circular flow, and finally flows from the extruded connecting protrusion 1004 into the connecting notch 5 to flush the solid phase into the sewage receiving tank.

[0076] The embodiments of the present invention disclose preferred embodiments, but are not limited thereto. A person skilled in the art can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. However, as long as they do not deviate from the spirit of the present invention, they are all within the protection scope of the present invention.

Claims

1. A chromatography device with backwashing function, characterized in that: The invention comprises a guide rail (1) and a chromatography component (2); the chromatography component (2) is arranged on the guide rail (1) via a support frame (3); the internal gap of the chromatography component (2) constitutes a chromatography channel, and a plurality of communication gaps (5) are arranged at equal intervals in a circular shape at both ends of the chromatography channel; the communication gaps (5) are sealed by a one-way sealing component (6); Wherein, the connecting gap (5) arranged relatively close to the input end of the chromatography channel is a forward flushing connecting channel; Furthermore, the communication gap (5) arranged relatively close to the output end of the chromatography channel is a backwash connection channel; The flushing system (4) is slidably arranged outside the chromatography component (2) and connected to the movable end of the guide rail (1); The flushing system (4) comprises a base connecting ring frame (7) slidably arranged outside the chromatography component (2); a plurality of connecting arms (8) are hingedly connected in a circular shape with equal spacing on one side of the base connecting ring frame (7); inner wall gaps of the plurality of connecting arms (8) arranged in a circular shape with equal spacing form a rotation adjustment cavity; a driving disk (9) is rotatably arranged inside the rotation adjustment cavity; and a fitting adjustment unit (10) is hingedly provided on the connecting arm (8); Wherein, a flushing connector connected to an external water purification pump is provided on the surface of any one of the fitting adjustment units (10); Wherein, a plurality of fitting adjustment units (10) distributed in an annular shape and at equal intervals are connected via a hinged telescopic tube assembly (11); Wherein, a plurality of the fitting adjustment units (10) are internally connected to the hinged telescopic tube assembly (11) to form a single-channel inner shaft perfusion and flushing structure; and the flushing connector is connected to the internal channel of the fitting adjustment unit (10); Wherein, the single-channel inner shaft perfusion and flushing structure has an extrusion-type connection state and a tangential-type loose state; When the single-channel inner shaft perfusion and flushing structure is in a tangentially loosened state, the guide rail (1) drives the flushing system (4) to slide toward the chromatographic channel input end of the connecting notch (5) to the forward flushing connection channel, and the single-channel inner shaft perfusion and flushing structure is adjusted to an extrusion-type connected state, so that the single-channel inner shaft perfusion and flushing structure is connected to the chromatographic component (2) to form a forward flushing structure for flushing the stationary phase inside the chromatographic component (2); When the single-channel inner shaft perfusion and flushing structure is in a tangentially loosened state, the guide rail (1) drives the flushing system (4) to slide toward the output end of the chromatography channel of the communication gap (5) to the reverse flushing connection channel, and the single-channel inner shaft perfusion and flushing structure is adjusted to an extrusion-type connected state, so that the single-channel inner shaft perfusion and flushing structure is connected to the chromatography component (2) to form a reverse flushing structure for flushing the stationary phase inside the chromatography component (2); The one-way sealing component (6) comprises a three-way sealing block (601) arranged inside the communication gap (5) via a spring (602); wherein the three-way sealing block (601) is provided with a "Y"-shaped communication port (6011) inside; the cross section of the three-way sealing block (601) is a trapezoidal structure; wherein the cross section of the communication gap (5) is spindle-shaped, and an auxiliary matching gap (501) with a larger outer side and a smaller inner side is provided on the cross section of the communication gap (5) relatively close to the outside of the chromatography receiving pipe (201); and the communication gap (5) is relatively close to the inclined surface of the three-way sealing block (601) and is matched with the inclination angle of the inclined surface of the three-way sealing block (601); The basic connection ring frame (7) is slidably arranged on the auxiliary connection shaft, and the basic connection ring frame (7) and the chromatography receiving pipe (201) are coaxially arranged; and an extension convex shaft is arranged on the side of the basic connection ring frame (7), and a push rod (703) is hingedly arranged on the extension convex shaft; The surface of the basic connecting ring frame (7) is provided with a plurality of hinge shafts (701) at equal intervals in a ring shape, and the hinge shaft (701) is rotatably provided with a limit block (702) in the shape of a "convex" character; The connecting arm (8) is hingedly arranged on the hinge shaft (701), and a relative gap between a plurality of the connecting arms (8) and a plurality of the hinge shafts (701) forms a rotation adjustment cavity; The surface of the driving disk (9) is provided with a plurality of inclined extrusion grooves (901) at equal intervals in a ring shape, and the side extension end of the driving disk (9) is hinged to the telescopic end of the push rod (703); The fitting adjustment unit (10) comprises a connecting block (1001); the connecting block (1001) is hingedly arranged at the end of the connecting arm (8), wherein the surface of the connecting block (1001) is provided with an extrusion pin that slidably cooperates with the extrusion groove (901); and the connecting block (1001) is provided with an arc portion (1002) and a straight portion (1003) in sequence on a side relatively close to the chromatography receiving pipe (201), and the arc portion (1002) and the straight portion (1003) are tangentially distributed, wherein the connecting The outer wall of the block (1001) on one side relatively close to the chromatography receiving pipe (201) is provided with an extruded communication protrusion (1004); wherein, the side of the connecting block (1001) relatively close to the adjacent connecting block (1001) is respectively provided with a hinged protrusion (1005) and a hinged receiving groove (1006); wherein, the interior of the connecting block (1001) is provided with an internal communication channel (1007) communicating with the hinged protrusion (1005), the hinged receiving groove (1006) and the extruded communication protrusion (1004); The hinged telescopic tube assembly (11) comprises a hollow rotating shaft sleeve A (1101) hingedly arranged on the hinged protrusion (1005); a hollow rotating shaft sleeve B (1103) is provided on one side of the hollow rotating shaft sleeve A (1101) via a telescopic tube (1102), and the hollow rotating shaft sleeve B (1103) is rotatably connected to the hinged receiving groove (1006).

2. A chromatography device with backwashing function according to claim 1, characterized in that: The two support frames (3) are symmetrically arranged on the guide rail (1), and the two support frames (3) are connected via at least one auxiliary connecting shaft.

3. A chromatography device with backwashing function according to claim 2, characterized in that: The chromatography component (2) comprises a chromatography receiving pipe (201) arranged on the support frame (3), and both ends of the chromatography receiving pipe (201) are connected to an external processing pipe via a diverter valve; hollow blocking plates (202) are symmetrically arranged on both axial sides inside the chromatography receiving pipe (201) and relatively located between the forward flushing connection channel and the reverse flushing connection channel, wherein a stationary phase is filled between the two blocking plates (202).

4. A chromatography device with backwashing function according to claim 3, characterized in that: The hollow rotating sleeve A (1101) and the hollow rotating sleeve B (1103) are both provided with a conveying hole on the outside, the size of which is smaller than the size of the internal connecting channel (1007).

5. The method for using a chromatography device with backwashing function according to claim 4, characterized in that: The following steps are involved: S1, installation process: connecting the two axial ends of the chromatography receiving pipe (201) in the chromatography device to the detection object delivery end and the sewage receiving tank through the diverter valve, and connecting the other end to the detection object processing receiving vessel and the sewage receiving tank through the diverter valve; and connecting the pump delivery end of the clean water tank to the flushing connector through the pipe; S2, chromatography treatment: by opening the detection object conveying end on one side of the input end of the chromatography receiving pipeline (201) and opening the detection object processing receiving container at the output end of the chromatography receiving pipeline (201), the chromatography detection is subjected to chromatography work through the solid phase; S3, flushing pretreatment: by closing the detection object conveying end on one side of the input end of the chromatography receiving pipeline (201) and closing the detection object processing receiving container at the output end of the chromatography receiving pipeline (201); S4, flushing and adjustment process: S4.

1. If positive flushing adjustment is performed after chromatography on low concentration test substances: First, the push rod (703) is driven to rotate the base connection ring frame (7) by the return stroke, and the extrusion pin is caused to rotate and deflect by the inclined extrusion groove (901), so that the connection block (1001) is adjusted synchronously, so that the connection block (1001) is unfolded, and then the flushing system (4) is driven to slide as a whole by the guide rail (1), so that the connection block (1001) moves relatively close to the forward flushing connection channel, and then the push rod (703) is driven to rotate the base connection ring frame (7) by the stroke, and the extrusion groove (901) is caused to rotate. The extrusion pin performs a rotational offset movement, causing the connection block (1001) to be adjusted synchronously, so that the connection block (1001) contracts, and at the same time the hollow rotating shaft sleeve A (1101) and the hollow rotating shaft sleeve B (1103) move closer, and the telescopic tube (1102) moves in compression, and then the extrusion connection protrusion (1004) is caused to squeeze the three-way blocking block (601) based on the rotational offset adjustment, so that the communication gap (5) is connected, and then the diverter valves of the detection object conveying end and the sewage receiving tank are closed, and the diverter valve of the detection object processing receiving vessel at the other end is opened; S4.

2. If backwashing and adjustment are performed after chromatography on high-concentration test substances: First, the push rod (703) is driven to rotate the base connection ring frame (7) by the return stroke, and the extrusion pin is caused to rotate and deflect by the inclined extrusion groove (901), so that the connection block (1001) is adjusted synchronously, so that the connection block (1001) is unfolded, and then the flushing system (4) is driven to slide as a whole by the guide rail (1), so that the connection block (1001) moves relatively close to the reverse flushing connection channel, and then the push rod (703) is driven to rotate the base connection ring frame (7) by the stroke, and the extrusion pin is caused to rotate and deflect by the inclined extrusion groove (901). The connecting block (1001) is rotated and offset, so that the connecting block (1001) is adjusted synchronously, so that the connecting block (1001) contracts, and at the same time the hollow rotating shaft sleeve A (1101) and the hollow rotating shaft sleeve B (1103) move closer, and the telescopic tube (1102) moves in compression, and then the extrusion connecting protrusion (1004) is squeezed on the three-way blocking block (601) based on the rotational offset adjustment, so that the connecting gap (5) is connected, and then the diverter valves of the detection object processing receiving container and the sewage receiving tank at the output end are closed, and the diverter valves of the detection object delivery end and the sewage receiving tank are opened; S5, flushing treatment: clean water is pumped into the flushing connector through the clean water tank pump, and is diverted through the internal connecting channel (1007) of the connecting block (1001) and poured into the connected hinged telescopic pipe assembly (11), forming a circular flow, and finally flows from the extruded connecting protrusion (1004) into the connecting notch (5) to flush the solid phase into the sewage receiving tank.

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