A microbial test piece production line and production method thereof

Abstract

The present invention relates to the field of microbial test sheet production, and more specifically, to a microbial test sheet production line, comprising a production line support frame, an upper substrate paper tray, a middle substrate paper tray and a lower substrate paper tray, wherein the production line support frame is provided with a lower substrate coating device, a lower substrate drying and dehumidifying device, a middle substrate punching and merging device, an upper film powder coating and merging device and a coding and cutting device in sequence from an inlet end to an outlet end; the lower substrate coating device coats a mixed liquid on the lower substrate paper; the middle substrate punching and merging device punches holes on the middle substrate paper, and the lower substrate paper coated with the mixed liquid passes through the lower substrate drying and dehumidifying device and is pressed with the punched middle substrate paper, and the pressed middle substrate paper and the lower substrate paper are transmitted to the upper film powder coating and merging device; the upper film powder coating and merging device presses the upper substrate paper coated with powder on the middle substrate paper; and the coding and cutting device codes and cuts the upper substrate paper on which the middle substrate paper and the lower substrate paper are pressed.

Description

Technical Field

[0001] The present invention relates to the field of microbial test piece production, and more specifically, to a microbial test piece production line and a production method thereof. Background Art

[0002] Food microbial indicators, such as total colony count and coliform group, are an important quality testing standard in the field of food safety, and are also important monitoring items for food risk prediction and hazard assessment.

[0003] At present, the mainstream method for detecting various microbial indicators is still the plate count method, but the test piece method has gradually been recognized and written into the national food safety standards, such as GB4789.2-2022 "National Food Safety Standard Food Microbiology Examination Colony Count Determination". Compared with the traditional plate count method, the test piece method eliminates a lot of labor-intensive and time-consuming auxiliary work such as culture medium preparation, sterilization, and vessel washing, and simplifies microbial detection into steps such as sample solution preparation, sample culture and counting. It is simple and convenient, and has strong practicality such as counting, and has great application prospects.

[0004] Among the commercialized test pieces for microbial detection, the Petrifil TM series with cold water gel as carrier produced by 3M Company of the United States has always been at the forefront of the world, with users all over the world. The microbial test pieces independently developed by my country are also mostly with cold water gel and non-woven fabric as carrier, but there are still some shortcomings, and the growth of colonies, typical color and counting accuracy need to be effectively improved. For example, a domestic manufacturer's total colony count test piece product with non-woven fabric as carrier still has atypical colony growth and color when used to detect Bacillus, resulting in inaccurate counting. At the same time, there is still a lot of room for improvement in the production process of test pieces for microbial detection. For example, taking the test piece product with cold water gel as carrier as an example, it can be divided into dry method and wet method according to the attachment form of the nutrient components of the bottom film in the product.

[0005] The dry method is that each component is attached to the base film in the form of powder, such as the process used in CN116948803A. This production process has the disadvantages that the powder is difficult to attach quantitatively and it is easy to cause dust pollution during production. The wet method is that each component is evenly dissolved in the liquid phase, and quantitative attachment can be achieved after coating it on the base film and drying it, such as US20200056219A1. This method overcomes the various defects of the dry method, but it causes new problems. For example, the volatilization of organic solvents causes pollution and harm to the environment and workshop personnel. In order to speed up the drying and volatilization of organic solvents, a certain merchant even selected highly harmful organic solvents such as isopropyl alcohol. Therefore, we are committed to developing a complete series of microbial test pieces and corresponding automated production processes, which are health-friendly to production personnel and environmentally friendly.

[0006] The core process of the test piece production line is the powder or liquid fixation of the upper and lower membranes. The current test piece production lines often solve this problem manually, which will lead to low production efficiency and an increase in the defective rate. Therefore, it is necessary to propose a microbial test piece production line and a production method thereof that can solve the above problems. Summary of the invention

[0007] In order to overcome at least one defect (deficiency) of the prior art, the present invention provides a microbial test piece production line and a production method thereof.

[0008] To solve the above technical problems, the technical solution of the present invention is as follows: a microbial test piece production line, comprising a production line support frame, an upper substrate paper tray for winding an upper substrate paper, a middle substrate paper tray for winding a middle substrate paper, and a lower substrate paper tray for winding a lower substrate paper;

[0009] The production line support frame is provided with a lower substrate coating device, a lower substrate drying and dehumidifying device, a middle substrate punching and merging device, an upper film powder coating and merging device and a coding and cutting device in sequence from the inlet end to the outlet end;

[0010] The lower substrate paper tray is arranged at the inlet end of the production line support frame, and the lower substrate paper tray is connected to the lower substrate coating device, and the lower substrate coating device coats the mixed liquid on the lower substrate paper;

[0011] The middle-layer substrate paper tray is connected to the middle-layer substrate punching merger, and the middle-layer substrate punching merger punches holes in the middle-layer substrate paper. The lower-layer substrate paper coated with the mixed liquid passes through the lower-layer substrate drying and dehumidifying device, and then passes through the middle-layer substrate punching merger to press the punched middle-layer substrate paper together. The pressed middle-layer substrate paper and the lower-layer substrate paper are transmitted to the upper-layer film powder coating and merging device.

[0012] The upper film powder coating merging device is connected to the upper substrate paper tray, and the upper film powder coating merging device presses the upper substrate paper coated with powder onto the middle substrate paper;

[0013] The coding and cutting device performs coding and cutting on the upper substrate paper laminated with the middle substrate paper and the lower substrate paper. The mixed liquid can be evenly coated on the lower substrate paper through the lower substrate coating device. After the mixed liquid is coated on the lower substrate paper, the water in the mixed liquid can be effectively removed after passing through the lower substrate drying dehumidifier. After punching, the middle substrate paper is laminated on the lower substrate paper, so that the middle substrate paper and the lower substrate paper form a stable and tightly bonded composite structure. After applying powder on the upper substrate paper, the upper substrate paper is laminated on the composite structure formed by the middle substrate paper and the lower substrate paper. After coding and cutting, the coding and cutting device can output the test piece. Since the test piece is produced in a fully automatic manner, the production efficiency can be improved while the defective rate of the microbial test piece can be reduced.

[0014] Furthermore, the lower substrate coating device includes a stirring motor, a stirring paddle, a stirring tank, a glass sight glass, a feeding cylinder, a digital micrometer, a coating device and a conveyor belt;

[0015] The lower substrate paper is conveyed to the bottom of the applicator via a conveyor belt;

[0016] The stirring paddle is arranged in the stirring tank, and the stirring motor is arranged on the top of the stirring paddle and drives the stirring paddle to rotate;

[0017] The glass sight glass is arranged at the bottom of the stirring tank and is connected to the stirring tank;

[0018] The material discharge cylinder is arranged at the bottom of the glass sight glass, and the liquid applicator is connected with the material discharge cylinder;

[0019] The digital micrometer is arranged at the outlet of the liquid coater, and controls the discharge amount of the liquid coater. The stirring motor drives the stirring paddle to rotate, so that the mixed liquid in the stirring tank can be stirred more evenly. The glass sight glass is arranged to facilitate production personnel to inspect and observe the use of the mixed liquid in the stirring tank. The discharge cylinder is controlled by a computer, which can control the discharge speed of the mixed liquid in the stirring tank. The digital micrometer on the liquid coater can be used to adjust the thickness of the liquid coating on the surface of the lower substrate paper.

[0020] Furthermore, the stirring paddle is hollow. Since the stirring paddle is hollow, bubbles can be avoided as much as possible in the mixed liquid during the stirring process, because the appearance of bubbles will make the coated surface uneven, thereby leading to the formation of unqualified products.

[0021] Further, the lower substrate drying dehumidifier includes a dehumidifier, a dehumidification box, a dry air inlet, a dry air outlet and a transmission base;

[0022] The dehumidification box is arranged on the production line support frame;

[0023] The dry air inlet and the dry air outlet are evenly arranged on the dehumidification box, and the dehumidifier is connected to the dry air inlet and the dry air outlet through pipelines to form a circulation loop;

[0024] The transmission base is arranged in the dehumidification box, and the lower substrate paper coated with the mixed liquid passes through the dehumidification box through the transmission base to remove the moisture of the mixed liquid. By continuously blowing dry gas into the dehumidification box, at this time, as long as the humidity, flow rate and temperature of the dry gas are controlled well, the moisture removal problem of the mixed liquid can be achieved.

[0025] Furthermore, the middle-layer substrate punching merger includes a middle-layer substrate paper pulling motor, a paper tape positioning cylinder, a punch, an easy-tear paper recovery tray, a release paper paper pulling motor, and a middle- and lower-layer paper merging and pressing roller arranged on a production line support frame;

[0026] The middle layer substrate paper comprises a middle layer stone paper and a release paper which are pressed together in sequence from top to bottom;

[0027] The middle-layer substrate paper pulling motor pulls the middle-layer substrate paper on the middle-layer substrate paper tray to the paper tape positioning cylinder for positioning;

[0028] The puncher is arranged at the end of the paper tape positioning cylinder, and the puncher punches holes in the positioned middle-layer substrate paper;

[0029] The release paper pulling motor is arranged at the end of the puncher. After the release paper pulling motor separates the middle-layer stone paper and the release paper, the release paper is wound in the easy-tear paper recovery plate. After the middle-layer stone paper is wound through the middle-lower paper merging and pressing roller, it is pressed against the lower-layer substrate paper output by the transmission base. The middle-layer substrate paper is punched by the puncher. The middle-layer substrate paper with holes is recycled into a roll under the action of the middle-layer substrate pulling motor and the easy-tear paper recovery plate, exposing one side of the middle-layer stone paper with strong adhesion. Then, under the action of the middle-lower paper merging and pressing roller, a stable and tightly bonded composite structure is formed.

[0030] Furthermore, it also includes a waste trough, which is arranged below the puncher. Through the setting of the waste trough, the discs cut by the puncher can be collected, which is simple and convenient.

[0031] Furthermore, the upper film powder coating and merging device includes an upper easy-tear paper recovery tray, an upper paper pulling cylinder, a lifting and lowering adjustment hand wheel, a powder stirring motor, a powder stirring paddle, a material storage tank, a powder outlet, a powder suction head, a powder suction pump, a blocking sheet and a merging roller arranged on a production line support frame;

[0032] The upper substrate paper comprises an upper release paper and a biaxially oriented polypropylene film which are pressed against each other in sequence from top to bottom. One end of the upper substrate paper is wound on the upper substrate paper tray, and the other end is guided by the upper paper pulling cylinder to roll the upper release paper in the upper easy-tear paper recovery paper tray, and the biaxially oriented polypropylene film is driven to the lower powder port and the merging roller in sequence.

[0033] The powder stirring paddle is arranged in the material storage tank, and the powder stirring motor is arranged on the powder stirring paddle;

[0034] One end of the powder lowering port is connected to the lifting and lowering adjustment hand wheel, and the other end is arranged at the bottom of the storage tank, and the blocking sheet is arranged at the bottom of the powder lowering port;

[0035] The powder suction head is arranged around the outer side of the powder outlet, and the powder suction head is connected to the powder suction pump;

[0036] The merging roller presses the biaxially oriented polypropylene film onto the middle-layer substrate paper to form a test sheet. The barrier sheet can be positioned 1 cm away from the edge of the powder outlet to cover the powder, thereby leaving adhesiveness so that it can form adhesion with the lower substrate paper. The lifting and lowering adjustment hand wheel can adjust the distance between the powder outlet and the biaxially oriented polypropylene film. If the distance is too large, a large amount of powder will be discharged. At this time, too much powder will remain on the biaxially oriented polypropylene film, which will cause a large working pressure of the subsequent powder suction pump to suck out all the residual powder. If the distance is too small, the powder outlet may directly stick to the biaxially oriented polypropylene film, causing it to break. At the same time, uneven powder application will occur, resulting in unqualified products.

[0037] Furthermore, a paper belt deflection corrector is provided on the outer side of the merging roller to prevent the biaxially oriented polypropylene film from deviating or twisting. The paper belt deflection corrector can be used to correct the deviation or twisting of the biaxially oriented polypropylene film, so that the biaxially oriented polypropylene film is straightened and does not deviate, thereby ensuring that the biaxially oriented polypropylene film can be better bonded to the middle layer of stone paper.

[0038] Furthermore, the coding and cutting device includes a coding and cutting platform, a cutting and positioning cylinder, a paper cutting cylinder, a paper cutting knife, a paper cutting and pulling cylinder and a paper cutting and pulling motor;

[0039] The cutting and pulling paper cylinder is connected to the cutting and pulling paper motor, and the cutting and pulling paper motor pulls the test paper sheet out of the merging roller;

[0040] The inkjet station is arranged on the cutting and positioning cylinder to inkjet the test paper;

[0041] The cutting and positioning cylinder abuts against the cutting and pulling cylinder to position the test paper after coding;

[0042] The paper cutting cylinder is arranged on the production line support frame, and the paper cutting knife is connected to the paper cutting cylinder. The paper cutting knife cuts the test piece paper after positioning and coding. When the test piece paper passes the inkjet terminal, the inkjet terminal starts to print according to the set batch number and content. At this time, the specified batch number and other information will be printed on the test piece paper. At this time, the test piece paper continues to move forward, and the cutting positioning cylinder starts to work to accurately position the test piece paper under the cutting knife. The cutting positioning cylinder fixes the test piece paper in the correct position of the cutting knife to ensure accurate cutting. When the positioning of the test piece paper is completed, the cutting cylinder starts to push the cutting knife for cutting. The cutting knife cuts the test piece paper according to the predetermined size. After the cutting is completed, the cutting cylinder resets the cutting knife.

[0043] The present invention also discloses a method for producing a microbial test piece, comprising the following steps:

[0044] S1, the conveyor belt conveys the lower substrate paper to the bottom of the coating device, and the digital micrometer controls the feeding amount of the coating device so that the mixed liquid is evenly coated on the lower substrate paper;

[0045] S2, transferring the lower substrate paper coated with the mixed liquid to the lower substrate drying dehumidifier to remove moisture from the mixed liquid;

[0046] S3, the puncher punches holes in the middle substrate paper to form sample adding slots, and the middle and lower layers of paper are combined with a pressing roller to press the punched middle substrate paper onto the lower substrate paper after the water of the mixed liquid is removed in S2;

[0047] S4, the height of the lower powder port is adjusted by the lifting and adjusting hand wheel, so that the powder is evenly applied on the upper substrate paper after passing through the lower powder port, and the powder suction head arranged around the outer side of the lower powder port sucks the excess powder back into the storage tank, and the merging roller presses the biaxially oriented polypropylene film on the middle substrate paper of step S3 to form a test sheet;

[0048] S5, the cutting and pulling paper cylinder drives the cutting and pulling paper motor to pull the test paper out of the merging roller, the cutting positioning cylinder positions the pulled test paper, the inkjet terminal inkjet codes the pulled test paper, and the cutting cylinder drives the paper cutter to cut the test paper into the required shape.

[0049] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0050] The microbial test piece production line disclosed in the present invention can evenly coat the mixed liquid on the lower substrate paper through the lower substrate coating device. After the mixed liquid is coated on the lower substrate paper, the lower substrate paper can effectively remove moisture in the mixed liquid after passing through the lower substrate drying dehumidifier. After punching, the middle substrate paper is pressed on the lower substrate paper, so that the middle substrate paper and the lower substrate paper form a stable and tightly bonded composite structure. After the upper substrate paper is powdered, the upper substrate paper is pressed on the composite structure formed by the middle substrate paper and the lower substrate paper. After the coding cutting device is coding cutting, the test piece can be output. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 It is the overall framework diagram of the microbial test piece production line in the present invention.

[0052] Figure 2 It is a schematic structural diagram of the lower substrate coating device in the present invention.

[0053] Figure 3 It is a structural schematic diagram of the lower substrate drying dehumidifier in the present invention.

[0054] Figure 4 It is a structural schematic diagram of the middle layer substrate punching merger in the present invention.

[0055] Figure 5 It is a structural schematic diagram of the upper film powder coating and merging device in the present invention.

[0056] Figure 6 It is a structural schematic diagram of the coding and cutting device in the present invention.

[0057] In the figure, 1 is an upper substrate paper tray, 2 is a middle substrate paper tray, 3 is a lower substrate paper tray, 4 is a lower substrate coating device, 5 is a lower substrate drying dehumidifier, 6 is a middle substrate punching merger, 7 is an upper film powder coating merger device, 8 is a coding cutting device, 9 is a stirring motor, 10 is a stirring paddle, 11 is a stirring tank, 12 is a glass sight glass, 13 is a feeding cylinder, 14 is a digital micrometer, 15 is a coating device, 16 is a conveyor belt, 17 is a dehumidifier, 18 is a dehumidification box, 19 is a drying air inlet, 20 is a drying air outlet, 21 is a transmission base, 22 is a middle substrate paper pulling motor, 23 is a paper tape positioning cylinder, 24 is a hole puncher, 25 is an easy-tear paper recovery tray, 26 is a release paper pulling motor, 27 is a middle and lower paper merging and pressing roller, 28 is an upper easy-tear paper recovery tray, 29 is an upper paper pulling cylinder, 30 is a lifting and adjusting hand wheel, 31 is a powder stirring motor, 32 is a powder stirring paddle, 33 is a storage tank, 34 is a powder outlet, 35 is a powder suction head, 36 is a powder suction pump, 37 is a blocking sheet, 38 is a merging roller, 39 is a spray dock, 40 is a cutting positioning cylinder, 41 is a paper cutting cylinder, 42 is a paper cutter, 43 is a cutting paper pulling cylinder, and 44 is a cutting paper pulling motor. DETAILED DESCRIPTION

[0058] The drawings are only used for illustrative purposes and should not be construed as limitations on this patent. In order to better illustrate this embodiment, some parts of the drawings may be omitted, enlarged, or reduced, and do not represent the size of the actual product. For those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted.

[0059] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. It can be said that the internal connection of two components is connected. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The technical solution of the present invention is further explained below in conjunction with the accompanying drawings and embodiments.

[0060] like Figure 1As shown, a microbial test piece production line comprises a production line support frame, an upper substrate paper disc 1 for winding an upper substrate paper, a middle substrate paper disc 2 for winding a middle substrate paper and a lower substrate paper disc 3 for winding a lower substrate paper; the production line support frame is provided with a lower substrate coating device 4, a lower substrate drying and dehumidifying device 5, a middle substrate punching and merging device 6, an upper film powder coating and merging device 7 and a coding and cutting device 8 in sequence from the inlet end to the outlet end; the lower substrate paper disc is arranged at the inlet end of the production line support frame, the lower substrate paper disc is connected to the lower substrate coating device, and the lower substrate coating device coats the mixed liquid on the lower substrate paper; the middle substrate paper disc is connected to the middle substrate punching and merging device, and the middle substrate punching and merging device punches the middle substrate paper, and the lower substrate paper coated with the mixed liquid passes through the lower substrate drying and dehumidifying device, and is pressed together with the punched middle substrate paper through the middle substrate punching and merging device, and the pressed middle substrate paper and the lower substrate paper are transmitted to the upper substrate The invention relates to a membrane powder coating merging device; the upper membrane powder coating merging device is connected to the upper substrate paper disc, and the upper membrane powder coating merging device presses the upper substrate paper coated with powder on the middle substrate paper; the coding and cutting device performs coding and cutting on the upper substrate paper pressed with the middle substrate paper and the lower substrate paper, and the mixed liquid can be evenly coated on the lower substrate paper through the lower substrate coating device, and after the lower substrate paper is coated with the mixed liquid, it can effectively remove the moisture in the mixed liquid after passing through the lower substrate drying and dehumidifier, and the middle substrate paper is pressed on the lower substrate paper after punching, so that the middle substrate paper and the lower substrate paper form a stable and tightly bonded composite structure, and after the upper substrate paper is coated with powder, the upper substrate paper is pressed on the composite structure formed by the middle substrate paper and the lower substrate paper, and the coding and cutting device can output the test piece after coding and cutting. Since the test piece is produced in a fully automatic way, the production efficiency can be improved while the defective rate of the microbial test piece can be reduced.

[0061] like Figure 2As shown, the lower substrate coating device includes a stirring motor 9, a stirring paddle 10, a stirring tank 11, a glass sight glass 12, a feeding cylinder 13, a digital micrometer 14, a coating device 15 and a conveyor belt 16; the lower substrate paper is conveyed to the bottom of the coating device by the conveyor belt; the stirring paddle is arranged in the stirring tank, the stirring motor is arranged on the top of the stirring paddle and drives the stirring paddle to rotate; the glass sight glass is arranged at the bottom of the stirring tank and is connected to the stirring tank; the feeding cylinder is arranged at the bottom of the glass sight glass, and the coating device is connected to the feeding cylinder; the digital micrometer is arranged at the outlet of the coating device, and the digital micrometer controls the feeding amount of the coating device. The motor drives the stirring paddle to rotate, so that the mixed liquid in the stirring tank can be stirred more evenly. The glass sight glass is set to facilitate production personnel to inspect and observe the use of the mixed liquid in the stirring tank. The feeding cylinder is controlled by a computer to control the discharge speed of the mixed liquid in the stirring tank, and the digital micrometer on the coating device can be used to adjust the thickness of the liquid coating on the surface of the lower substrate paper. Among them, the stirring paddle is hollow. Since the stirring paddle is hollow, during the stirring process, bubbles can be avoided as much as possible in the mixed liquid, because the appearance of bubbles will make the coated surface uneven, thus leading to the formation of unqualified products.

[0062] like Figure 3 As shown, the lower substrate drying dehumidifier includes a dehumidifier 17, a dehumidification box 18, a dry air inlet 19, a dry air outlet 20 and a transmission base 21; the dehumidification box is arranged on a production line support frame; the dry air inlet and the dry air outlet are evenly arranged on the dehumidification box, and the dehumidifier is connected to the dry air inlet and the dry air outlet through pipelines to form a circulation loop; the transmission base is arranged in the dehumidification box, and the lower substrate paper coated with the mixed liquid passes through the dehumidification box through the transmission base to remove the moisture of the mixed liquid, and by continuously blowing dry gas into the dehumidification box, at this time, as long as the humidity, flow rate and temperature of the dry gas are well controlled, the problem of removing the moisture of the mixed liquid can be achieved.

[0063] like Figure 4As shown, the middle-layer substrate punching merger includes a middle-layer substrate paper pulling motor 22, a paper tape positioning cylinder 23, a puncher 24, an easy-tear paper recovery tray 25, a release paper paper pulling motor 26 and a middle-lower paper merging and pressing roller 27 arranged on a production line support frame; the middle-layer substrate paper includes a middle-layer stone paper and a release paper that are pressed against each other in sequence from top to bottom; the middle-layer substrate paper pulling motor pulls the middle-layer substrate paper on the middle-layer substrate paper tray to the paper tape positioning cylinder for positioning; the puncher is arranged at the end of the paper tape positioning cylinder, and the puncher punches holes in the positioned middle-layer substrate paper; the release paper pulling motor is arranged at the end of the puncher, and the release paper pulling motor pulls the middle-layer stone paper and the release paper After separation, the release paper is wound on the easy-tear paper recovery plate, the middle-layer stone paper is wound on the middle-lower-layer paper merging and compacting roller and then pressed against the lower-layer substrate paper output by the transmission base, and the middle-layer substrate paper is punched by a hole puncher. The middle-layer substrate paper with holes is recycled into a roll under the action of the middle-layer substrate paper pulling motor and the easy-tear paper recovery plate, exposing one side of the middle-layer stone paper with strong adhesion, and then under the action of the middle-lower-layer paper merging and compacting roller, a stable and tight composite structure is formed. In addition, it also includes a waste trough, which is arranged below the hole puncher. Through the setting of the waste trough, the discs cut by the hole puncher can be collected, which is simple and convenient.

[0064] like Figure 5As shown in the figure, the upper-layer film powder coating merging device includes an upper-layer easy-tear paper recycling paper tray 28, an upper-layer paper pulling cylinder 29, a lifting adjustment handwheel 30, a powder stirring motor 31, a powder stirring paddle 32, a storage tank 33, a powder discharging port 34, a powder suction head 35, a powder suction pump 36, a blocking piece 37, and a merging roller 38 arranged on the production line support frame; the upper-layer base paper includes an upper-layer release paper and a biaxially oriented polypropylene film that are sequentially pressed together from top to bottom. One end of the upper-layer base paper is wound around the upper-layer base paper tray, and the other end, under the guidance of the upper-layer paper pulling cylinder, winds the upper-layer release paper into the upper-layer easy-tear paper recycling paper tray, and drives the biaxially oriented polypropylene film into the powder discharging port and the merging roller in sequence; the powder stirring paddle is arranged in the storage tank, and the powder stirring motor is arranged on the powder stirring paddle; one end of the powder discharging port is connected to the lifting adjustment handwheel, and the other end is arranged at the bottom of the storage tank. The blocking piece is arranged at the bottom of the powder discharging port; the powder suction head is arranged around the outside of the powder discharging port, and the powder suction head is connected to the powder suction pump; the merging roller presses the biaxially oriented polypropylene film onto the middle-layer base paper. Through the setting of the blocking piece, the blocking piece can be positioned at a position 1 cm away from the edge of the powder discharging port, so as to make the powder cover it, thereby leaving adhesiveness so that it can form adhesion with the lower-layer base paper. The lifting adjustment handwheel can adjust the distance between the powder discharging port and the biaxially oriented polypropylene film. If the distance is too large, it will cause a large amount of powder discharging. At this time, there is too much powder remaining on the biaxially oriented polypropylene film, which will cause a large working pressure for the powder suction pump in the subsequent powder suction work and fail to suck all the remaining powder. If the distance is too small, it is possible that the powder discharging port directly adheres to the biaxially oriented polypropylene film and causes it to break. At the same time, there will be uneven powder coating and unqualified products. Among them, a paper tape deviation rectifier for preventing the biaxially oriented polypropylene film from deviating or twisting is arranged outside the merging roller. Through the setting of the paper tape deviation rectifier, it can be used to rectify the deviation or twisting of the biaxially oriented polypropylene film, so that the biaxially oriented polypropylene film is straightened and does not deviate, thereby ensuring that the biaxially oriented polypropylene film can better adhere to the middle-layer stone paper.

[0065] As Figure 6As shown, the coding and cutting device includes a coding station 39, a cutting positioning cylinder 40, a paper cutting cylinder 41, a paper cutting knife 42, a cutting paper pulling cylinder 43 and a cutting paper pulling motor 44; the cutting paper pulling cylinder is connected to the cutting paper pulling motor, and the cutting paper pulling motor pulls the test piece out of the merging roller; the coding station is arranged on the cutting positioning cylinder to code the test piece; the cutting positioning cylinder is against the cutting paper pulling cylinder to position the test piece after coding; the paper cutting cylinder is arranged on the production line support frame, and the paper cutting knife is connected to the paper cutting cylinder, and the paper cutting knife positions and codes the test piece after coding. The test piece is cut. When the test piece passes the inkjet printing station, the inkjet printing station starts to print according to the set batch number and content. At this time, the specified batch number and other information will be printed on the test piece. At this time, the test piece continues to move forward, and the cutting positioning cylinder starts to work to accurately position the test piece under the cutting knife. The cutting positioning cylinder fixes the test piece in the correct position of the cutting knife to ensure accurate cutting. When the test piece is positioned, the cutting cylinder starts to push the cutting knife for cutting. The cutting knife cuts the test piece according to the predetermined size. After cutting is completed, the cutting cylinder resets the cutting knife.

[0066] The present invention also discloses a method for producing a microbial test piece, comprising the following steps:

[0067] S1, the conveyor belt conveys the lower substrate paper to the bottom of the coating device, and the digital micrometer controls the feeding amount of the coating device so that the mixed liquid is evenly coated on the lower substrate paper;

[0068] S2, transferring the lower substrate paper coated with the mixed liquid to the lower substrate drying dehumidifier to remove moisture from the mixed liquid;

[0069] S3, the puncher punches holes in the middle substrate paper to form sample adding slots, and the middle and lower layers of paper are combined with a pressing roller to press the punched middle substrate paper onto the lower substrate paper after the water of the mixed liquid is removed in S2;

[0070] S4, the height of the lower powder port is adjusted by the lifting and adjusting hand wheel, so that the powder is evenly applied on the upper substrate paper after passing through the lower powder port, and the powder suction head arranged around the outer side of the lower powder port sucks the excess powder back into the storage tank, and the merging roller presses the biaxially oriented polypropylene film on the middle substrate paper of step S3 to form a test sheet;

[0071] S5, the cutting and pulling paper cylinder drives the cutting and pulling paper motor to pull the test paper out of the merging roller, the cutting positioning cylinder positions the pulled test paper, the inkjet terminal inkjet codes the pulled test paper, and the cutting cylinder drives the paper cutter to cut the test paper into the required shape.

[0072] Example

[0073] In this embodiment, the complete test piece is composed of two layers of stone paper structure in the middle and lower layers, the coating of culture components and gel in the groove, and the upper film of biaxially oriented polypropylene film (BOPP) with gel and indicator or other transparent film with certain air permeability but impermeability. Such a structure forms a complete microbial culture detection system when the liquid sample is added to the groove. The carbon source, nitrogen source, growth factor, salt ion and other components required for microbial growth are provided by the structure in the lower groove. The cold water gel in the upper and lower structures provides a stable medium for the growth of microorganisms into focused colonies. The indicator makes the microorganisms color and is easy to count and observe. Therefore, such a culture detection system, except for the indicator, is very similar to the plate pouring method in the national food safety standard. The bacteria do not stay on the surface of agar or cold water gel, but are mixed together to form a stable focused colony morphology.

[0074] When it is necessary to produce microbial test pieces, the stirring motor in the lower substrate coating device drives the stirring paddle to stir in the stirring tank in order to make the nutrients and gel in the stirring tank in a uniform suspension state. Therefore, by driving the stirring paddle through the stirring motor to stir, it can be ensured that the components in the formula are evenly distributed. Among them, the paddle of the stirring paddle is a hollow structure. The purpose of this is to make sure that during the stirring process, when the mixed liquid is about to be pushed into the coating by the feeding cylinder, bubbles will appear as little as possible, because the appearance of bubbles will make the coating surface uneven and form unqualified products. Of course, defoaming agents are also introduced into the formula. The defoaming agents have been selected and evaluated in the laboratory and are required not to affect the growth of microorganisms. In the production of this test piece, the defoaming agent used is food-grade concentrated polyglycol, which can effectively control the formation of foam from protein components such as beef extract powder and peptone.

[0075] Among them, the mixing tank puts the nutrients and gel mixture prepared in the preparation room into it, and its working volume can be 0 to 10L. For example, 5L is the most suitable for each feeding. Each test piece consumes 0.68ml of liquid, and about 7350 test pieces can be made. At the efficiency of 1000 pieces per hour, it only takes 7 hours to feed. In fact, the microbial test piece production line does not work in an absolutely sterile production environment. Therefore, the feeding process cannot ensure sterile operation, so the culture components in the mixing tank cannot be retained for too long to prevent the possible reproduction of a large number of bacteria.

[0076] The glass sight glass is provided to facilitate production personnel to inspect and observe the usage of the mixed liquid in the mixing tank. Although there is no original usage alarm mechanism, production personnel can decide the timing of feeding through their own experience or timer, and the timing of feeding does not need to be very accurate. The discharge cylinder is controlled by a computer, which can control the discharge speed of the mixed liquid in the mixing tank. This discharge speed is also directly related to the speed of the conveyor belt and the consumption rate of the mixed liquid in the applicator. Therefore, the start, operation and pause of the applicator are all achieved by the linkage of the discharge cylinder and the applicator under computer control.

[0077] The liquid coater is the core module for stirring the microbial culture system evenly and quantitatively applying it to the lower substrate paper of the test piece. Its working mode is to control the lower substrate paper to move at a uniform speed under the liquid coater through the speed of the conveyor belt. The lower substrate paper is the lower stone paper, and the feeding cylinder controls the amount of liquid. The two balance adjustment knobs of the digital micrometer on the liquid coater can be used to adjust the thickness of the liquid coating on the surface of the lower stone paper. The accuracy can be controlled at about 10μm. For the mixed liquid with a fixed formula, we need to evenly apply the mixed liquid to the surface of the 250μm lower stone paper with a thickness of 100μm. The lower stone paper is a carbonated The polymer of calcium and polyethylene pe has the characteristics of paper. It has a certain hardness to maintain the flatness of the test piece, but it is impermeable and has a certain air permeability. Microorganisms also need a certain amount of gas to grow, so it is suitable as the bottom substrate for culturing bacteria on the test piece. When using powder spraying (dry method), the lower layer of stone paper needs to be prepared with pressure-sensitive adhesives such as acrylate isooctyl ester before the culture medium and other powders can be fixed on the lower layer of stone paper, but there will be a certain amount of shedding. Liquid coating does not need this preparation because the cold gel added to the mixed components also has a certain viscosity. After being applied to the lower layer of stone paper that has not been prepared with glue and dried to remove moisture, it still It can form a stable coating that is not easy to fall off. After being simplified, this process can reduce the use of isooctyl acrylate and its solvents, and is also very environmentally friendly. The lower layer of stone paper is cut by a cutting machine before it goes on the production line. Because the width of the test piece is 96mm, the width of the lower layer of stone paper roll is 96mm and the length is about 450 meters. Because the lower layer of stone paper is very heavy, it is not suitable to be rolled too long, which is not conducive to the manual fixing of the lower layer of stone paper on the line, so the applicator needs to adjust the thickness to 100μm under the control of the digital micrometer balance adjustment button. In addition, there is another parameter, which is the coating width. Although the test piece is 96mm wide, the applicator only adjusts the middle 70 mm for coating, leaving 13mm blank on both sides. There are two reasons for this. First, the coating after the coating liquid is dried (the 70mm segment in the middle) is compared with the uncoated blank lower stone paper (the 13mm segments at both ends). When entering the next process section and bonding with the 60mm diameter intermediate lower stone paper with the prepared glue, the 13mm lower stone paper at both ends is more tightly bonded, which can ensure better adhesion of the two layers of the test piece and more stability of the test piece; second, the 70mm segment can ensure that the circular hole with an aperture of 60mm is covered with the components of the culture system, and the nutrients and gel in this aperture are the effective action area. Therefore, the coating liquid is not applied to the entire 96 width, but only to the 70 width. According to the overall linkage effect, the lower substrate coating device can achieve an output of more than 5,000 pieces per hour, but because the entire production process is affected by the lower substrate drying dehumidifier and the upper film powder coating merging device, the final production capacity is maintained at a speed of 1,000 pieces per hour.In terms of performance, the stone paper coating material produced by this independently designed lower substrate coating device is both stable and uniform, and is suitable for mass production of test pieces.

[0078] Since the microbial test sheet adopts a wet production process, after the lower stone paper is evenly coated with the mixed liquid, it will be transported to the lower substrate drying dehumidifier for drying. Compared with the dry process of fixing the powder on the pressure-sensitive adhesive, the wet method is to first dissolve the culture system and the gel in sterile deionized water to form a uniform mixed liquid, and then apply it to the lower stone paper substrate. Therefore, the wet method needs to remove the moisture in the mixed system after the coating is formed. By using deionized water as the solvent of the powder system, although the problems of uniform dissolution, personnel-friendliness and environmental protection are solved, it is different from the dry method. Compared with other manufacturers who use organic solvents as the medium for drying, the drying and volatilization of water is obviously more difficult than that of organic volatile solvents. Therefore, it is necessary to specially design a targeted lower substrate drying dehumidifier to solve this problem. This lower substrate drying dehumidifier mainly relies on the introduction of 45-degree dry gas into the dehumidification box. There are multiple parallel dry air inlets and dry air outlets in the dehumidification box. The dehumidifier continuously blows dry gas into the dehumidification box to dry the moisture in the lower stone paper. At this time, as long as the humidity, flow rate and temperature of the dry gas are controlled, the moisture removal problem of the mixed liquid on the coating can be achieved.

[0079] Specifically, the lower substrate drying dehumidifier can perform rotary dehumidification on the lower stone paper through dry gas. In order to achieve an efficiency of 1500 sheets per hour, the dry gas wind speed and its processing air volume can be set to 580m 3 The regeneration air volume is set to 175m per hour. 3 The working parameters are set to 400m per hour. 3 per hour; its working dry gas temperature can reach 60 degrees Celsius, and the working parameters are set to 43-35 degrees Celsius; its rated dehumidification capacity is 3.3kg per hour, and the moisture content of each test piece is 0.48g. Theoretically, the rotary dehumidifier can dehumidify about 6,000 sheets. Under this parameter setting, it can mass produce 1,500 sheets per hour.

[0080] By continuously circulating the dry gas, the lower layer of stone paper within 3 meters can be completely dried, and the temperature in the dehumidification box is set to 45 degrees Celsius. Because when the temperature is higher than this temperature, the lower layer of stone paper may become uneven during the drying process, and when it is added to the next process section for bonding, it will cause the problem of loose bonding. Therefore, the present invention sets the temperature in the dehumidification box to 45 degrees Celsius.

[0081] After the water in the lower layer of stone paper is removed, the lower layer of stone paper will be transported to the middle layer substrate punching merger, which will first punch the middle layer substrate paper to form a groove with a 60mm aperture. This groove will be used as a sample loading slot for subsequent test pieces for adding and testing 1ml samples. This middle layer substrate paper is composed of a 400μm thick middle layer of stone paper with glue and a release paper coated with silicone oil. The release paper coated with silicone oil is to facilitate separation from the sticky middle layer of stone paper after punching, so that the sticky middle layer of stone paper can form a stable composite structure with the dried coated lower layer of stone paper.

[0082] When the puncher is pressed down onto the middle layer of stone paper, round holes will appear on the middle layer of stone paper. The punched round pieces will fall off from below into the waste bin as garbage, leaving only the middle layer of stone paper tape with round holes spaced 26mm apart, which can be used to cut into test pieces with grooves 13mm away from the edge later.

[0083] After the middle-layer stone paper is punched, the release paper coated with silicone oil is recycled into a roll under the action of the middle-layer substrate paper pulling motor and the easy-tear paper recovery disk, exposing one side of the middle-layer stone paper with strong adhesion. Then, the coated lower-layer stone paper and the middle-layer stone paper are combined under the action of the middle-lower-layer paper merging and pressing roller to form a stable and tightly bonded composite structure. At this point, the punching and bonding actions are completed, and the base of the test piece is also formed.

[0084] In the present invention, the powder in the storage tank is a mixed powder of cold water gel and a developer, and the upper substrate paper tray is rolled with an upper release paper and a biaxially oriented polypropylene film (BOPP) which are pressed together in sequence. The biaxially oriented polypropylene film has a thickness of 40 μm and a width of 100 mm, while the width of the lower stone paper is 96 mm, that is, the extra 4 mm is reserved for the user to open the biaxially oriented polypropylene film for sample addition; the thickness of the biaxially oriented polypropylene film is 5-12 μm, and the main component of the prepared glue is acrylic acid. Isooctyl acrylate / ethyl acrylate / methyl acrylate / acrylamide, the ratio is 67 / 11 / 19.4 / 2.6. The glue preparation process is to evenly apply the liquid glue on the substrate, and then dry it at high temperature. The liquid glue originally contains a main agent and a solvent. In the above ratio, isooctyl acrylate / ethyl acrylate / methyl acrylate are the main agents, and acrylamide is the solvent, so the content of acrylamide after drying is very small. Experiments have shown that the main agent component on the substrate after drying of the glue preparation in this formula has no obvious effect on the growth of microorganisms.

[0085] The upper substrate paper starts to be supplied from the upper substrate paper tray and is pulled by the upper paper pulling cylinder. The upper paper pulling cylinder controls the conveying speed and direction of the upper substrate paper to ensure that the upper substrate paper moves forward smoothly, and one side of the upper release paper is collected by the upper easy-tear paper recycling paper tray, and the lifting and lowering adjustment hand wheel is to adjust the distance between the powder outlet of the storage tank and the biaxially oriented polypropylene film, because if the distance is too large, it will cause a large amount of powder to be discharged from the powder outlet, and too much powder will remain on the adhesive biaxially oriented polypropylene film, which will cause the subsequent powder suction pump to have a large working pressure for suction, and fail to suck out all the residual powder, which will cause the powder to be scattered to the lower layer after bonding with the middle and lower stone paper. If the distance is too small, the powder outlet may directly stick to the biaxially oriented polypropylene film, causing it to break, and at the same time, uneven powder application will occur, making the product unqualified.

[0086] After adjusting the lifting and lowering adjustment hand wheel to the optimal distance as mentioned above and putting on the biaxially oriented polypropylene film, the powder added in the storage tank is evenly sprinkled onto the adhesive biaxially oriented polypropylene film through the powder outlet. The specification of the powder outlet is 7.2cm*2.7cm in length and width. Then the biaxially oriented polypropylene film with powder is transmitted to the powder suction head which is only 5cm away from the powder outlet horizontally. The powder suction head will suck away the excess residual powder and return it to the storage tank. This cycle continues. During the powder discharging and powder suction process, the blocking piece is positioned at a distance of 1cm from the edge of the powder outlet to leave a distance of 1cm for the adhesive biaxially oriented polypropylene film to prevent the powder from covering it, thereby leaving the adhesiveness so that it can form adhesion with the lower stone paper. The volume of the storage tank is 13L, but 500g is generally selected as the normal working feeding amount. Calculated at 0.25g powder coating per piece, 2000 test pieces can be produced. If the production speed is set to 1000 pieces / hour, 500g powder can be used for 2 hours. If the amount of powder added exceeds this 500g setting, it will cause a large amount of powder to be discharged from the lower powder port, and even powder explosion. This is also related to the air volume of the powder return system of the powder suction port. Of course, if it is too little, manual powder adding work will be frequently increased. The role of the paper belt deviation corrector is to ensure that the above-mentioned biaxially oriented polypropylene film coated with powder maintains the correct track during the transmission process, prevent the paper belt from deviating or twisting, and affect the bonding with the lower stone-paper composite structure.

[0087] When the biaxially oriented polypropylene film is bonded to the middle and lower composite structures, the powder-coated biaxially oriented polypropylene film paper tape is bonded to the lower stone paper composite structure through a merging roller. This bonding is mainly due to the 1 cm wide structure with stickiness left by the above-mentioned biaxially oriented polypropylene film. The merging roller performs the final combination and compaction to form a complete composite structure of two layers of stone paper and one layer of biaxially oriented polypropylene film.

[0088] Finally, the operator sets the batch number of this production on the inkjet printer, enters the batch number on the control panel of the inkjet printer, and sets the inkjet terminal's inkjet coding content to the corresponding batch number and other information that needs to be printed. When the cutting and pulling paper motor starts to run, the uncut test piece of paper is pulled out from the upper film powder coating and merging device, and the cutting and pulling paper cylinder cooperates with the operation of the cutting and pulling paper motor to ensure that the uncut test piece of paper moves forward smoothly and evenly. When the uncut test piece of paper passes through the inkjet terminal, the inkjet terminal starts printing according to the set batch number and content. At this time, the uncut test piece of paper will be printed with the specified batch number and other information, and qualified paper continues to move forward, and the cutting positioning air cylinder stops printing. The cylinder starts to work and accurately positions the uncut test piece of paper under the cutting knife. The cutting positioning cylinder fixes the uncut test piece of paper in the correct position of the cutting knife to ensure accurate cutting. When the uncut test piece of paper is positioned, the cutting positioning cylinder starts to push the cutting knife for cutting. The cutting knife cuts the uncut test piece of paper according to the predetermined size. After cutting, the cutting positioning cylinder resets the cutting knife. The cut test piece is transmitted to the collection area by the motor. The operator can view the working status of each module in real time through the monitoring system to ensure the smooth operation of the production line. The machine modules are regularly maintained and adjusted to ensure the accuracy and consistency of coding, positioning and cutting.

[0089] In the figure, the description of the positional relationship is only for illustrative purposes and should not be understood as a limitation on this patent; obviously, the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations on the implementation methods of the present invention. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made on the basis of the above description. It is not necessary and impossible to list all the implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A microbial test sheet production line, comprising a production line support frame, an upper substrate paper tray for winding an upper substrate paper, a middle substrate paper tray for winding a middle substrate paper, and a lower substrate paper tray for winding a lower substrate paper, characterized in that: The production line support frame is provided with a lower substrate coating device, a lower substrate drying and dehumidifying device, a middle substrate punching and merging device, an upper film powder coating and merging device and a coding and cutting device in sequence from the inlet end to the outlet end; The lower substrate paper tray is arranged at the inlet end of the production line support frame, and the lower substrate paper tray is connected to the lower substrate coating device, and the lower substrate coating device coats the mixed liquid on the lower substrate paper; the lower substrate coating device includes a stirring motor, a stirring paddle, a stirring tank, a glass sight glass, a feeding cylinder, a digital micrometer, a coating device and a conveyor belt, the glass sight glass is arranged at the bottom of the stirring tank and is connected to the stirring tank, the feeding cylinder is arranged at the bottom of the glass sight glass, the coating device is connected to the feeding cylinder, the digital micrometer is arranged at the outlet of the coating device, and the digital micrometer controls the feeding amount of the coating device. The lower substrate drying dehumidifier includes a dehumidifier, a dehumidification box, a dry air inlet, a dry air outlet and a transmission base; The middle-layer substrate paper tray is connected to the middle-layer substrate punching merger, and the middle-layer substrate punching merger punches the middle-layer substrate paper. After the lower-layer substrate paper coated with the mixed liquid passes through the lower-layer substrate drying and dehumidifying device, it is pressed together with the punched middle-layer substrate paper through the middle-layer substrate punching merger, and the pressed middle-layer substrate paper and the lower-layer substrate paper are transmitted to the upper film powder coating and merging device; the middle-layer substrate punching merger includes a middle-layer substrate paper pulling motor, a paper tape positioning cylinder, a puncher, an easy-tear paper recovery disk, a release paper paper pulling motor, and a middle-lower-layer paper merging and pressing roller arranged on a production line support frame; The upper film powder coating and merging device comprises an upper easy-tear paper recycling tray, an upper paper pulling cylinder, a lifting and adjusting hand wheel, a powder stirring motor, a powder stirring paddle, a storage tank, a lower powder port, a powder suction head, a powder suction pump, a blocking plate and a merging roller arranged on a production line support frame; the upper film powder coating and merging device is connected to the upper substrate paper tray, and the upper film powder coating and merging device presses the upper substrate paper coated with powder onto the middle substrate paper, one end of the lower powder port is connected to the lifting and adjusting hand wheel, and the other end is arranged at the bottom of the storage tank, the blocking plate is arranged at the bottom of the lower powder port, the powder suction head is arranged around the outside of the lower powder port, and the powder suction head and the powder suction pump are connected to each other; The coding and cutting device performs coding and cutting on the upper substrate paper laminated with the middle substrate paper and the lower substrate paper.

2. The microbial test piece production line according to claim 1, characterized in that: The lower substrate paper is conveyed to the bottom of the applicator via a conveyor belt; The stirring paddle is arranged in the stirring tank, and the stirring motor is arranged on the top of the stirring paddle and drives the stirring paddle to rotate.

3. The microbial test piece production line according to claim 2, characterized in that: The stirring paddle is hollow.

4. The microbial test piece production line according to claim 1, characterized in that: The dehumidification box is arranged on the production line support frame; The dry air inlet and the dry air outlet are evenly arranged on the dehumidification box, and the dehumidifier is connected to the dry air inlet and the dry air outlet through pipelines to form a circulation loop; The transmission base is arranged in the dehumidification box, and the lower substrate paper coated with the mixed liquid passes through the dehumidification box through the transmission base to remove the moisture of the mixed liquid.

5. The microbial test piece production line according to claim 4, characterized in that: The middle layer substrate paper comprises a middle layer stone paper and a release paper which are pressed together in sequence from top to bottom; The middle-layer substrate paper pulling motor pulls the middle-layer substrate paper on the middle-layer substrate paper tray to the paper tape positioning cylinder for positioning; The puncher is arranged at the end of the paper tape positioning cylinder, and the puncher punches holes in the positioned middle-layer substrate paper; The release paper pulling motor is arranged at the end of the puncher. After the release paper pulling motor separates the middle layer stone paper and the release paper, the release paper is wound in the easy-to-tear paper recovery tray. The middle layer stone paper is wound through the middle and lower layer paper merging and pressing roller and then pressed against the lower layer substrate paper output by the transmission base.

6. The microbial test piece production line according to claim 5, characterized in that: Also included is a waste trough disposed below the hole punch.

7. The microbial test piece production line according to claim 6, characterized in that: The upper substrate paper comprises an upper release paper and a biaxially oriented polypropylene film which are pressed against each other in sequence from top to bottom. One end of the upper substrate paper is wound on the upper substrate paper tray, and the other end is guided by the upper paper pulling cylinder to roll the upper release paper in the upper easy-tear paper recovery paper tray, and the biaxially oriented polypropylene film is driven to the lower powder port and the merging roller in sequence. The powder stirring paddle is arranged in the material storage tank, and the powder stirring motor is arranged on the powder stirring paddle; The merging roller presses the biaxially oriented polypropylene film onto the middle layer substrate paper to form a test sheet paper.

8. The microbial test piece production line according to claim 7, characterized in that: A paper belt deviation corrector is arranged on the outer side of the merging roller to prevent the biaxially oriented polypropylene film from deviating or twisting.

9. The microbial test piece production line according to claim 7, characterized in that: The coding and cutting device comprises a coding station, a cutting positioning cylinder, a paper cutting cylinder, a paper cutting knife, a paper cutting and pulling cylinder and a paper cutting and pulling motor; The cutting and pulling paper cylinder is connected to the cutting and pulling paper motor, and the cutting and pulling paper motor pulls the formed test sheet paper out of the merging roller; The inkjet station is arranged on the cutting and positioning cylinder to inkjet the paper sheets forming the test sheets; The cutting and positioning cylinder abuts against the cutting and pulling cylinder to position the test sheet after coding. The paper cutting cylinder is arranged on a production line support frame, and the paper cutting knife is connected to the paper cutting cylinder. The paper cutting knife cuts the paper pieces forming the test pieces after positioning and coding.

10. A method for producing a microbial test piece, characterized in that: The steps include: S1, the stirring motor in the lower substrate coating device drives the hollow structure stirring paddle to stir in the stirring tank, and the mixture is transported to the coating device through the feeding cylinder, and the conveyor belt conveys the lower substrate paper to the bottom of the coating device, and the digital micrometer controls the feeding amount of the coating device, so that the mixed liquid is evenly coated on the lower substrate paper; S2, the lower substrate paper coated with the mixed liquid is transferred to the lower substrate drying dehumidifier to remove the moisture of the mixed liquid, and the 45-degree dry air enters the dehumidification box, and the dehumidification box is provided with a plurality of parallel dry air inlets and dry air outlets. The dehumidifier continuously blows dry air into the dehumidification box to dry the moisture in the lower substrate; S3, the puncher punches holes in the middle substrate paper to form sample loading slots, and the middle and lower substrates are combined with a pressing roller to press the punched middle substrate paper onto the lower substrate paper after the water content of the mixed liquid is removed in S2; S4, the height of the lower powder port is adjusted by the lifting and adjusting hand wheel, so that the powder is evenly applied on the upper substrate paper after passing through the lower powder port, and the powder suction head arranged around the outer side of the lower powder port sucks the excess powder back into the storage tank, and the merging roller presses the biaxially oriented polypropylene film on the middle substrate paper in step S3 to form a test sheet; S5, the cutting and pulling paper cylinder drives the cutting and pulling paper motor to pull the test paper sheet out from the merging roller, the cutting positioning cylinder positions the pulled test paper sheet, the inkjet terminal inkjet codes the pulled test paper sheet, and the cutting cylinder drives the paper cutter to cut the test paper sheet into the required shape.

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