Biochip

Abstract

This application discloses a biochip, including a main body and multiple protrusions. The main body forms a first cavity and a second cavity, an inlet port and an outlet port. The second cavity is located below and communicates with the first cavity, and has multiple third sidewalls. The main body has a stepped surface, which includes a first region and a second region. Each third sidewall is provided with at least one first region. Multiple protrusions are located in the first cavity, and the protrusions are connected to and protrude from the first regions. The second region is a blank area. In this design, each third sidewall of the second cavity is provided with at least one first region, and the protrusions connect to the first regions, so that the entire upper circumference of the second cavity is provided with protrusions. On the one hand, multiple protrusions can adsorb suspended matter from multiple directions, improving the adsorption effect of suspended matter. On the other hand, multiple protrusions can constrain external organs within the second cavity, thus fixing the position of the external organs.

Description

Technical Field

[0001] This application relates to the field of organ-on-a-chip technology, and more particularly to a biochip. Background Technology

[0002] Currently, brain-like organoid models have been widely used to model human nervous system diseases, but there are still many problems, such as organoids cultured under long-term oscillations not being in the same working state as those in actual operation, and the inability to simulate the organoid growth process under extreme conditions.

[0003] The primary purpose of this chip design is to cultivate in vitro organ models under special environmental conditions, such as brain-like organ model cultivation in aerospace environments, to minimize the impact of these special conditions on their growth status. Summary of the Invention

[0004] This application provides a biochip to mitigate the impact of experimental materials undergoing extreme conditions such as hypergravity, weightlessness, and violent oscillations on cultured in vitro organ models during the upward (launch) process, microgravity culture, and downward (retrieval) process.

[0005] This application provides a biochip, comprising:

[0006] The main body has a first cavity, a second cavity located below and communicating with the first cavity, and an inlet and an outlet communicating with the first cavity and spaced apart from each other. The second cavity has a plurality of third sidewalls connected in sequence. The main body has a stepped surface located between the first cavity and the second cavity and facing the first cavity. The stepped surface includes a first area and a second area connecting the first area. Each third sidewall is provided with at least one first area.

[0007] Multiple protrusions are located in the first cavity, and the protrusions are connected to the first area one by one and protrude from the first area. The second area is a blank area.

[0008] In some embodiments, the body forms a plurality of spaced second cavities, all of which are located below and communicate with the first cavity, and the stepped surface includes a first region corresponding to each second cavity, each first region being connected to a protrusion.

[0009] In some embodiments, multiple second cavities are arranged in an array along the row and column directions, with the row and column directions perpendicular to the thickness direction of the body.

[0010] In some embodiments, the first cavity has two first sidewalls arranged opposite each other in the row direction and two second sidewalls arranged opposite each other in the column direction. Each first sidewall is connected between the two second sidewalls, and the liquid inlet and liquid outlet are respectively provided at the junction of one of the first sidewalls and the two second sidewalls.

[0011] In some embodiments, the plurality of protrusions includes a first protrusion and a second protrusion, wherein the height of the first protrusion is less than the height of the first cavity, and the height of the second protrusion is equal to the height of the first cavity;

[0012] Along the row direction, a first protrusion is provided between every two adjacent columns of second cavities; along the row direction, a first protrusion is provided between the second cavity located at the edge and the first sidewall;

[0013] Along the column direction, a first protrusion and two second protrusions are provided between each two adjacent rows of second cavities, and the first protrusion is located between the two second protrusions along the row direction; along the column direction, a first protrusion is provided between the second cavity located at the edge and the second sidewall.

[0014] In some embodiments, the second protrusion extends along the row direction to the adjacent first sidewall.

[0015] In some embodiments, the plurality of protrusions includes a first protrusion, the height of which is less than the height of the first cavity.

[0016] In some embodiments, the plurality of protrusions includes a second protrusion, the height of which is equal to the height of the first cavity.

[0017] In some embodiments, the second region connects at least two sides of the first region.

[0018] In some embodiments, the liquid inlet is located on the stepped surface.

[0019] In some embodiments, the liquid outlet is located on the stepped surface.

[0020] In some embodiments, the first cavity has a first sidewall, and the liquid inlet and liquid outlet are respectively provided on opposite sides of the first sidewall.

[0021] In some embodiments, the body includes:

[0022] Base plate;

[0023] A middle plate, a bottom plate located below the middle plate and forming a second cavity between them; and

[0024] A cover plate is placed above the middle plate, forming a first cavity between the two.

[0025] In some embodiments, the upper surface of the intermediate plate is provided with an upper groove, and the inner wall of the upper groove forms a first cavity between the lower surface of the cover plate.

[0026] In some embodiments, the lower surface of the intermediate plate is provided with a lower groove, and a second cavity is formed between the inner wall of the lower groove and the upper surface of the bottom plate.

[0027] In this embodiment of the biochip, the second chamber is located below the first chamber, and both the inlet and outlet ports are connected to the first chamber. Fluid (e.g., culture medium) entering through the inlet port will first enter the first chamber, and then flow from the first chamber into the second chamber below it and the outlet port. Suspended matter in the fluid entering through the inlet port will at least partially adhere to the protrusions in the first chamber as it flows towards the outlet port, thus mitigating the problem of suspended matter accumulating at the outlet port and causing blockage.

[0028] In the above design, each third sidewall of the second cavity is provided with at least one first region, and the protrusions are connected to the first region one by one and protrude from the first region, so that the entire upper circumference of the second cavity is provided with protrusions. On the one hand, multiple protrusions can adsorb suspended matter from multiple directions, thereby improving the adsorption effect of suspended matter. On the other hand, multiple protrusions can constrain the external organ in the second cavity, thereby fixing the position of the external organ.

[0029] The aforementioned stepped surface also includes a second zone connecting the first zone. The second zone is a blank zone, allowing the fluid to pass through the blank zone, that is, to pass near the protrusion, which facilitates the adsorption of suspended matter in the fluid onto the protrusion and improves the adsorption effect of suspended matter. Attached Figure Description

[0030] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0031] Figure 1 These are schematic diagrams of the structure of the biochip provided in some embodiments of this application;

[0032] Figure 2 yes Figure 1 The diagram shows the exploded structure of a biochip.

[0033] Figure 3 yes Figure 1 A schematic diagram of the structure of the intermediate plate in a biochip is shown;

[0034] Figure 4 yes Figure 1 A schematic diagram of a partial perspective structure of a biochip is shown.

[0035] Figure 5 yes Figure 4 Schematic diagram of the cross-sectional structure along the AA direction;

[0036] Figure 6 yes Figure 4 A schematic diagram of the cross-sectional structure along the BB direction.

[0037] Explanation of reference numerals in the attached figures:

[0038] 1. Biochip;

[0039] 10. Main body; 11. First cavity; 111. First sidewall; 112. Second sidewall; 12. Second cavity; 121. Third sidewall; 13. Liquid inlet channel; 131. Liquid inlet connector; 132. Liquid inlet port; 14. Liquid outlet channel; 141. Liquid outlet connector; 142. Liquid outlet port; 15. Stepped surface; 151. First zone; 152. Second zone; 16. Base plate; 17. Intermediate plate; 171. Upper groove; 172. Lower groove; 18. Cover plate;

[0040] 20. Protrusion; 21. First protrusion; 22. Second protrusion;

[0041] 30. Liquid inlet line; 31. Liquid inlet pipe; 32. Liquid inlet filter; 33. Luer female connector; 34. Luer male connector;

[0042] 40. Liquid outlet pipeline; 41. Liquid outlet pipe; 42. Liquid outlet filter; 43. Luer female connector; 44. Luer male connector;

[0043] 50. Parts to remove air bubbles;

[0044] x: row direction; y: column direction; z: thickness direction. Detailed Implementation

[0045] To make the objectives, technical solutions, and advantages of this application clearer, the embodiments of this application will be described in further detail below with reference to the accompanying drawings.

[0046] In the following description, when referring to the accompanying drawings, the same numbers in different drawings denote the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with this application. Rather, they are merely examples of apparatuses and methods consistent with some aspects of this application as detailed in the appended claims.

[0047] Please see Figures 1 to 6 This application provides a biochip 1, which includes a main body 10 and a plurality of protrusions 20.

[0048] The main body 10 comprises a first cavity 11, a second cavity 12 located below and communicating with the first cavity 11, and liquid inlet 131 and liquid outlet 141 communicating with and spaced apart from the first cavity 11. The second cavity 12 has a plurality of third sidewalls 121 connected in sequence. The main body 10 has a stepped surface 15 located between the first cavity 11 and the second cavity 12 and facing the first cavity 11. The stepped surface 15 includes a first region 151 and a second region 152 connecting the first region 151. Each third sidewall 121 is correspondingly provided with at least one first region 151. A plurality of protrusions 20 are located in the first cavity 11, and the protrusions 20 are connected to the first regions 151 one by one and protrude from the first regions 151. The second region 152 is a blank area.

[0049] The second chamber 12 is located below the first chamber 11. Both the inlet port 131 and the outlet port 141 are connected to the first chamber 11. Fluid (e.g., culture medium) entering through the inlet port 131 will first enter the first chamber 11, and then flow from the first chamber 11 into the second chamber 12 below it and the outlet port 141. Suspended matter in the fluid entering through the inlet port 131 will at least partially adhere to the protrusions 20 within the first chamber 11 as it flows towards the outlet port 141, thus mitigating the problem of suspended matter accumulating at the outlet port 141 and causing blockage.

[0050] In the above design, each third sidewall 121 of the second cavity 12 is provided with at least one first region 151, and the protrusions 20 are connected to the first region 151 one by one and protrude from the first region 151, so that the entire upper circumference of the second cavity 12 is provided with protrusions 20. On the one hand, multiple protrusions 20 can adsorb suspended matter from multiple directions, thereby improving the adsorption effect of suspended matter. On the other hand, multiple protrusions 20 can constrain the external organs within the second cavity 12, thereby fixing the position of the external organs.

[0051] Each of the third sidewalls 121 of the aforementioned second cavity 12 is provided with at least one first region 151, and the protrusions 20 are connected to and protrude from the first region 151 in a one-to-one correspondence. This can be: (See reference) Figure 4 The second cavity 12 has four third sidewalls 121 connected in sequence, and each third sidewall 121 is provided with at least one protrusion 20. The number of protrusions 20 provided on each third sidewall 121 may be equal or unequal, and this application does not limit this. If a third sidewall 121 is provided with at least two protrusions 20, these at least two protrusions 20 may be spaced apart along the extending direction of the corresponding third sidewall 121 to achieve a better effect of fixing the external organs within the second cavity 12.

[0052] The aforementioned stepped surface 15 also includes a second region 152 connecting the first region 151. The second region 152 is a blank area, allowing fluid to pass through the blank area, that is, to pass near the protrusion 20, which facilitates the adsorption of suspended matter in the fluid onto the protrusion 20 and improves the adsorption effect of suspended matter.

[0053] The second region 152 can be connected to one side of the first region 151, or to at least both sides of the first region 151. If the second region 152 is connected to one side of the first region 151, fluid can pass through that side of the first region 151, allowing the protrusion 20 to adsorb suspended matter in the fluid on that side. If the second region 152 is connected to at least both sides of the first region 151, fluid can pass through that side of the first region 151, allowing the protrusion 20 to adsorb suspended matter in the fluid on those sides, thus improving the adsorption effect on suspended matter.

[0054] In this embodiment of the application, the second region 152 connects to the entire circumference of the first region 151. That is, the first region 151 is completely wrapped by the second region 152, which can ensure that fluid passes through the entire circumference of the first region 151 and that the entire circumference of the protrusion 20 can contact the fluid, thereby achieving the adsorption of suspended matter in the fluid.

[0055] The main body 10 has multiple spaced second cavities 12, all of which are located below and connected to the first cavity 11. Each second cavity 12 can be used to place an in vitro organ, allowing the biochip 1 to simultaneously culture multiple in vitro organs.

[0056] Furthermore, the stepped surface 15 includes a first region 151 corresponding to each second cavity 12, and each first region 151 is connected to a protrusion 20. Thus, the protrusion 20 can confine the extracorporeal organ within the corresponding second cavity 12, preventing multiple extracorporeal organs from agglomerating and adhering, thereby preventing necrosis. The second cavity 12 can undergo anti-adhesion treatment, for example, by immersing it in an anti-adhesion rinsing solution for 10 minutes, enabling 3D growth of brain organoids without the need for vibration.

[0057] Furthermore, when multiple spaced second cavities 12 are formed on the main body 10, for each second cavity 12, each third sidewall 121 is correspondingly provided with at least one first region 151, and the protrusions 20 are connected to and protrude from the first regions 151 in a one-to-one correspondence. That is, the entire circumference of the upper part of each second cavity 12 is provided with protrusions 20, which can achieve adsorption of suspended matter from multiple directions, improve the adsorption effect of suspended matter, and improve the restraint effect on external organs.

[0058] It is understood that the first region 151 and the protrusion 20 corresponding to the two adjacent third sidewalls 121 of two adjacent second cavities 12 can be shared or set separately. See the embodiment of this application for reference. Figure 4 The two adjacent second cavities 12 share the first region 151 and protrusion 20 corresponding to the two adjacent third sidewalls 121, so that the structure design of the biochip 1 is more compact and more second cavities 12 can be set in the same size to realize the culture of more in vitro organs.

[0059] The plurality of second cavities 12 on the main body 10 can be randomly arranged, or they can be arranged in an array along the row direction x or the column direction y. In this embodiment, the plurality of second cavities 12 on the main body 10 are arranged in an array along the row direction x and the column direction y, and the row direction x and the column direction y are perpendicular to the thickness direction z of the main body 10. The positions of the row direction x and the column direction y can be switched and are not limited thereto.

[0060] The first cavity 11 has two first sidewalls 111 arranged opposite each other along the row direction x and two second sidewalls 112 arranged opposite each other along the column direction y. Each first sidewall 111 is connected between two second sidewalls 112. The above design arranges multiple second cavities 12 in an array along the row direction x and column direction y, which allows for a regular arrangement of the multiple second cavities 12 below the first cavity 11. This facilitates the flow of culture medium entering through the first cavity 11 into each of the lower second cavities 12 and also promotes a regular structural design for each second cavity 12. For example, Figure 4 In each second cavity 12, there are four third sidewalls 121, two of which are arranged opposite each other along the row direction x, and the other two are arranged opposite each other along the column direction y. The two third sidewalls 121 arranged opposite each other along the row direction x can be parallel to the first sidewall 111, and the two third sidewalls 121 arranged opposite each other along the column direction y can be parallel to the second sidewall 112.

[0061] The inlet port 131 and outlet port 141, which communicate with the first cavity 11, can be respectively arranged on opposite sides of the first sidewall 111. Specifically, the inlet port 131 and outlet port 141 can be respectively arranged at the junction of one of the first sidewalls 111 and the two second sidewalls 112. It can be understood that the inlet port 131 and outlet port 141 are respectively arranged at two adjacent corners of the first cavity 11, which can separate the inlet port 131 and outlet port 141 as much as possible, so that the fluid entering through the inlet port 131 can reach the second cavity 12 and then flow out through the outlet port 141.

[0062] It should be noted that the liquid inlet port 131 and the liquid outlet port 141 can also be respectively set at two opposite corners of the first cavity 11 to meet different usage requirements. This application embodiment does not limit this.

[0063] The inlet port 131 can be located on the stepped surface 15. Since the fluid enters the first cavity 11 through the inlet port 131, placing the inlet port 131 on the stepped surface 15 at the bottom of the first cavity 11 helps to keep the fluid entering the first cavity 11 at a low position, enabling it to flow efficiently and promptly into the second cavity 12 below the stepped surface 15. It is understood that the inlet port 131 can also be located on the side wall of the first cavity 11, such as the first side wall 111 or the second side wall 112, etc., and there is no limitation on this.

[0064] In some embodiments, the main body 10 further includes a liquid inlet channel 13, which communicates with the first cavity 11 via a liquid inlet port 131. A liquid inlet port 132 communicating with the liquid inlet channel 13 is formed on the surface of the main body 10. This facilitates the introduction of fluid from the surface of the main body 10 into the liquid inlet channel 13, allowing the fluid to enter the first cavity 11 within the main body 10, and provides better sealing compared to related technologies that require opening the cap to add liquid.

[0065] In some embodiments, at least a portion of the inlet channel 13 is curved. Designing the inlet channel 13 to be at least partially curved helps to lengthen the inlet channel 13, extending the path of the fluid into the first chamber 11. A longer path for the fluid into the first chamber 11 results in a better de-bubbling effect. For example, see... Figure 4 The liquid inlet channel 13 may include an inner ring section and two semi-outer ring sections disposed around the inner ring section and connected to the inner ring section. The fluid in the liquid inlet channel 13 can flow through one half of the outer ring section, the inner ring section and the other half of the outer ring section in sequence, thereby extending the path of the fluid into the first chamber 11 and improving the degassing effect.

[0066] In some embodiments, the biochip 1 further includes a degassing element 50 communicating with the liquid inlet channel 13. The degassing element 50 can be any degassing element from the related art, and will not be described in detail here.

[0067] The liquid outlet 141 is located on the stepped surface 15. Since the fluid exits the first chamber 11 via the liquid outlet 141, placing the liquid outlet 141 on the stepped surface 15 at the bottom of the first chamber 11 facilitates its low position within the first chamber 11, enabling timely and efficient drainage of the fluid. It is understood that the liquid outlet 141 can also be located on the side wall of the first chamber 11, such as the first side wall 111 or the second side wall 112, etc., and this is not limited.

[0068] In some embodiments, the main body 10 further forms a liquid outlet channel 14, which communicates with the first cavity 11 through a liquid outlet port 141, and a liquid outlet port 142 communicating with the liquid outlet channel 14 is formed on the surface of the main body 10. This facilitates the discharge of fluid from the surface of the main body 10.

[0069] The liquid outlet channel 14 and the liquid inlet channel 13 are independent of each other, ensuring that the channels for fluid entering and exiting the first chamber 11 are independent and will not cause interference. For example, see... Figure 4 The liquid outlet channel 14 may include a short ring section, which may be located below the inner ring section and two semi-outer ring sections of the liquid inlet channel 13, so as to be independent of the inner ring section and two semi-outer ring sections to which the liquid enters 13.

[0070] In some embodiments, the inlet channel 13 and the outlet channel 14 may be located on the same side of the first cavity 11 so that the arrangement of the inlet channel 13 and the outlet channel 14 does not interfere with the first cavity 11 and the second cavity 12. For example, see Figure 4 Both the inlet channel 13 and the outlet channel 14 are located on one side of one of the first sidewalls 111 of the first cavity 11; that is, both the inlet channel 13 and the outlet channel 14 are located on the right / left side of the first cavity 11. Of course, both the inlet channel 13 and the outlet channel 14 can also be located on one side of one of the second sidewalls 112 of the first cavity 11; that is, both the inlet channel 13 and the outlet channel 14 are located on the front / rear side of the first cavity 11, and there is no limitation on this.

[0071] In some embodiments, the inlet port 132 of the inlet channel 13 and the outlet port 142 of the outlet channel 14 may be located on the same side of the main body 10, so that the inlet and outlet of the first cavity 11 can be realized on the same side of the biochip 1. For example, see Figure 4 The inlet port 132 of the inlet channel 13 and the outlet port 142 of the outlet channel 14 are both located on the right side of the main body 10 (e.g., the right sidewall or right end face of the main body 10). Of course, the inlet port 132 of the inlet channel 13 and the outlet port 142 of the outlet channel 14 can also be located on the left side (e.g., the left sidewall or left end face of the main body 10), the front side (e.g., the front sidewall or front end face of the main body 10), or the rear side (e.g., the rear sidewall or rear end face of the main body 10).

[0072] See Figure 5 and Figure 6 The main body 10 includes a base plate 16, an intermediate plate 17, and a cover plate 18. The base plate 16 is disposed below the intermediate plate 17, and a second cavity 12 is formed between the two. The cover plate 18 is disposed above the intermediate plate 17, and a first cavity 11 is formed between the two. By disassembling the main body 10 into a structure including the base plate 16, the intermediate plate 17, and the cover plate 18, it is beneficial to process and shape the second cavity 12 and the first cavity 11.

[0073] The base plate 16 and the intermediate plate 17 can be connected by adhesive or other means, and the cover plate 18 and the intermediate plate 17 can be connected by adhesive or other means, without limitation. Highly biocompatible medical-grade pressure-sensitive adhesives can be used for bonding, without limitation.

[0074] In some embodiments, the lower surface of the intermediate plate 17 is provided with a lower groove 172, and a second cavity 12 is formed between the inner wall of the lower groove 172 and the upper surface of the base plate 16. That is, by providing a groove only on the intermediate plate 17, the second cavity 12 can be formed when the intermediate plate 17 and the base plate 16 are connected, which can simplify the manufacturing process. In other embodiments, the groove can also be provided on the upper surface of the base plate 16, or the groove can be provided on both the upper surface of the base plate 16 and the lower surface of the intermediate plate 17 to form the second cavity 12, and there is no limitation on this.

[0075] In some embodiments, see Figure 5 and Figure 6 The base plate 16 can be configured to correspond to the second chamber 12, the inlet channel 13, and the outlet channel 14; that is, the base plate 16 is approximately from... Figure 5 The second cavity 12 on the left side of the intermediate plate 17 extends to the inlet channel 13 and outlet channel 14 on its right side. At this time, the portion of the base plate 16 corresponding to the inlet channel 13 and outlet channel 14 (i.e., the right side of the base plate 16) can form at least a portion of the inlet channel 13 and / or at least a portion of the outlet channel 14 with the intermediate plate 17. In other embodiments, the base plate 16 may only correspond to the second cavity 12; that is, the base plate 16 is generally located at... Figure 5 The left side of the middle plate 17.

[0076] In some embodiments, the upper surface of the intermediate plate 17 is provided with an upper groove 171, and a first cavity 11 is formed between the inner wall of the upper groove 171 and the lower surface of the cover plate 18. That is, by providing a groove only on the intermediate plate 17, the first cavity 11 can be formed when the intermediate plate 17 and the cover plate 18 are connected, which can simplify the manufacturing process. In other embodiments, the groove can also be provided on the lower surface of the cover plate 18, or the groove can be provided on both the lower surface of the cover plate 18 and the upper surface of the intermediate plate 17 to form the first cavity 11, and there is no limitation on this.

[0077] In some embodiments, see Figure 5 and Figure 6 The cover plate 18 can be provided only for the first cavity 11; that is, the cover plate 18 is approximately located in Figure 5 The left side of the middle plate 17. In other embodiments, the cover plate 18 may also be provided corresponding to the first cavity 11 and the liquid inlet channel 13 and the liquid outlet channel 14; that is, the cover plate 18 is approximately from the left side of the middle plate 17. Figure 5The liquid inlet channel 13 and liquid outlet channel 14 extend from the first cavity 11 on the left side of the middle plate 17 to its right side. In this embodiment, the cover plate 18 is only provided corresponding to the first cavity 11; that is, the cover plate 18 is approximately located at... Figure 5 The left side of the intermediate plate 17 is provided so that the right side of the intermediate plate 17 can be provided with a degassing component 50 that communicates with the liquid inlet channel 13.

[0078] The shape of the protrusion 20 can be arbitrary. For example, the cross-section of the protrusion 20 can be approximately circular, square, etc., without limitation. Along the extension direction of the protrusion 20, its cross-section can remain unchanged or change; for example, from one end of the protrusion 20 connecting the first region 151 to the other end, its cross-section can gradually increase, gradually decrease, or partially increase and partially decrease, etc., without limitation.

[0079] In some embodiments, the plurality of protrusions 20 include a first protrusion 21, the height of which is less than the height of the first cavity 11. Designing the height of the first protrusion 21 to be less than the height of the first cavity 11 can reduce the obstruction of fluid flow by the arrangement of the first protrusion 21 and improve the fluid exchange efficiency of the biochip 1.

[0080] In some embodiments, the plurality of protrusions 20 include a second protrusion 22, the height of which is equal to the height of the first cavity 11. Thus, the second protrusion 22 not only serves the functions described above for adsorbing suspended matter in the fluid and fixing the position of external organs within the second cavity 12, but also provides support for the cover plate 18, thereby enhancing the structural strength of the biochip 1.

[0081] It is understandable that the multiple protrusions 20 can all be the first protrusion 21, all be the second protrusion 22, or partly be the first protrusion 21 and partly be the second protrusion 22, without any limitation.

[0082] In this embodiment, the plurality of protrusions 20 include both a first protrusion 21 and a second protrusion 22. Specifically, see [reference needed]. Figure 3 and Figure 4Along the row direction x, a first protrusion 21 is provided between every two adjacent rows of second cavities 12; along the row direction x, a first protrusion 21 is provided between the second cavity 12 located at the edge and the first sidewall 111. Along the column direction y, a first protrusion 21 and two second protrusions 22 are provided between every two adjacent rows of second cavities 12, and the first protrusion 21 is located between the two second protrusions 22 along the row direction x; along the column direction y, a first protrusion 21 is provided between the second cavity 12 located at the edge and the second sidewall 112. The above design provides a first protrusion 21 around the entire circumference of each second cavity 12, which can reduce the obstruction when fluid enters the second cavity 12 from the entire circumference of the second cavity 12, and improve the adsorption effect of suspended matter around the entire circumference of the second cavity 12 and the fixation effect on external organs inside the second cavity 12. Along the column direction y, each pair of adjacent rows of second chambers 12 is provided with a first protrusion 21 and two second protrusions 22. The first protrusion 21 is located between the two second protrusions 22 along the row direction x. This facilitates the flow of fluid through the second protrusions 22 to the liquid outlet 141 directly via the first sidewall 111 after the liquid inlet 131 is disconnected. This allows the fluid to reach multiple second chambers 12 before flowing out through the liquid outlet 141.

[0083] Furthermore, see Figure 4 The second protrusion 22 can extend along the row direction x to the adjacent first sidewall 111 to improve the structural stability of the second protrusion 22.

[0084] In some embodiments, see Figure 1 and Figure 2 The biochip 1 also includes a liquid inlet conduit 30, which includes a liquid inlet pipe 31 and a liquid inlet filter 32. The liquid inlet pipe 31 is connected to the liquid inlet port 132 on the surface of the main body 10 and the liquid inlet filter 32. In this way, liquid can be introduced into the first cavity 11 through the liquid inlet pipe 31 connected to the liquid inlet filter 32, resulting in better sealing.

[0085] In some embodiments, the inlet pipe 31 and the inlet filter 32, as well as the inlet pipe 31 and the main body 10, can be connected via Luer connectors to improve the sealing performance between the inlet pipe 31 and the inlet filter 32, and between the inlet pipe 31 and the main body 10, thus meeting high sealing requirements. Specifically, the inlet pipe 31 and the inlet filter 32 can be connected via an interference fit at both ends of a Luer female connector 33; for example, the inlet pipe 31 can be connected to the threaded end of the Luer female connector 33 with an interference fit, and the inlet filter 32 can be connected to the pagoda end of the Luer female connector 33 with an interference fit. Specifically, the inlet pipe 31 and the main body 10 can be connected via an interference fit at both ends of a Luer male connector 34, which will not be elaborated further.

[0086] In some embodiments, the inlet filter 32 can be a Millex 0.22μm filter, the Luer female connector 33 can be a 1.6mm Luer female connector, and the Luer male connector 34 can be a 1.6mm Luer male connector. The inlet tube 31 can be a medical-grade silicone tube.

[0087] In some embodiments, see Figure 1 and Figure 2 The biochip 1 also includes a liquid outlet conduit 40, which includes a liquid outlet pipe 41 and a liquid outlet filter 42. The liquid outlet pipe 41 is connected to the liquid outlet port 142 on the surface of the main body 10 and the liquid outlet filter 42. In this way, the fluid in the first chamber 11 can be discharged through the liquid outlet pipe 41 connected to the liquid outlet filter 42, resulting in better sealing.

[0088] In some embodiments, the outlet pipe 41 and the outlet filter 42, as well as the outlet pipe 41 and the main body 10, can be connected via Luer connectors to improve the sealing performance between the outlet pipe 41 and the outlet filter 42, and between the outlet pipe 41 and the main body 10, thus meeting high sealing requirements. Specifically, the outlet pipe 41 and the outlet filter 42 can be connected via an interference fit at both ends of a Luer female connector 43; for example, the outlet pipe 41 can be interference-fitted to the threaded end of the Luer female connector 43, and the outlet filter 42 can be interference-fitted to the pagoda end of the Luer female connector 43. Specifically, the outlet pipe 41 and the main body 10 can be connected via an interference fit at both ends of a Luer male connector 44, which will not be elaborated further.

[0089] In some embodiments, the outlet filter 42 can be a Millex 0.22μm filter, the Luer female connector 43 can be a 1.6mm Luer female connector, and the Luer male connector 44 can be a 1.6mm Luer male connector. The outlet tube 41 can be a medical-grade silicone tube.

[0090] In the description of this application, it should be understood that the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances. Furthermore, in the description of this application, unless otherwise stated, "multiple" means at least two, for example, two, three, four, etc. "And / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. The character " / " generally indicates that the preceding and following related objects are in an "or" relationship.

[0091] The above-disclosed embodiments are merely preferred embodiments of this application and should not be construed as limiting the scope of this application. Therefore, any equivalent variations made in accordance with the claims of this application shall still fall within the scope of this application.

Claims

1. A biochip, characterized by, The main body is formed with a first cavity, a second cavity below the first cavity and communicating with the first cavity, a liquid inlet communication port and a liquid outlet communication port communicating with the first cavity and spaced from each other, the second cavity has a plurality of third side walls connected in sequence; the main body has a step surface between the first cavity and the second cavity and facing the first cavity, the step surface comprises a first area and a second area connecting the first area, and each third side wall is provided with at least one first area corresponding thereto; A plurality of protrusions are arranged in the first cavity, each protrusion is connected to the first area corresponding thereto and protrudes from the first area, the second area is a blank area, and each first area is connected to a protrusion. The main body is formed with a plurality of second cavities spaced from each other, all the second cavities are below the first cavity and communicate with the first cavity, and the step surface comprises the first area corresponding to each second cavity.

2. The biochip according to claim 1, wherein The plurality of second cavities are arranged in a row direction and a column direction, and the row direction, the column direction and the thickness direction of the main body are perpendicular to each other.

3. The biochip according to claim 2, wherein The first cavity has two first side walls arranged opposite in the row direction and two second side walls arranged opposite in the column direction, each first side wall is connected between two second side walls, 4. The biochip according to claim 3, wherein The liquid inlet communication port and the liquid outlet communication port are respectively arranged at the junction of one first side wall and two second side walls. The plurality of protrusions comprise first protrusions and second protrusions, the height of the first protrusion is less than the height of the first cavity, and the height of the second protrusion is equal to the height of the first cavity; 5. The biochip according to claim 4, wherein In the row direction, the first protrusion is arranged between every two adjacent columns of second cavities; in the row direction, the first protrusion is arranged between the second cavity at the edge and the first side wall. In the column direction, the first protrusion and two second protrusions are arranged between every two adjacent rows of second cavities, and the first protrusion is located between the two second protrusions in the row direction; in the column direction, the first protrusion is arranged between the second cavity at the edge and the second side wall. The second protrusion extends to the adjacent first side wall in the row direction.

6. The biochip according to claim 5, wherein The plurality of protrusions comprise first protrusions, and the height of the first protrusion is less than the height of the first cavity; 7. The biochip according to claim 1, wherein And / or, the plurality of protrusions comprise second protrusions, and the height of the second protrusion is equal to the height of the first cavity. The second area connects at least two sides of the first area; 8. The biochip according to claim 1, wherein And / or, the liquid inlet communication port is arranged on the step surface; And / or, the liquid outlet communication port is arranged on the step surface; And / or, the first cavity has a first side wall, and the liquid inlet communication port and the liquid outlet communication port are respectively arranged on opposite sides of the first side wall. The main body comprises:

9. The biochip according to claim 1, wherein a bottom plate; an intermediate plate, the bottom plate is arranged below the intermediate plate and forms the second cavity therebetween; and a cover plate arranged above the intermediate plate and forming the first cavity therebetween. An upper surface of the intermediate plate is provided with an upper groove, and an inner wall of the upper groove and a lower surface of the cover plate form the first cavity therebetween; 10. The biochip according to claim 9, wherein ​ And / or, the lower surface of the intermediate plate is provided with a lower groove, and the inner wall of the lower groove and the upper surface of the bottom plate form the second cavity.

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