Co-assembled nanoarray surface enhanced Raman scattering substrate and preparation method and application thereof

Abstract

The present invention discloses a co-assembled nano-array surface enhanced Raman scattering substrate, which relates to the field of biological detection technology. The co-assembled nano-array surface enhanced Raman scattering substrate is obtained by assembling No. 1 nano-gold particles into the gaps of No. 2 nano-gold particle arrays to improve the hot spots and sensitivity of the surface enhanced Raman scattering substrate, obtaining a gold film, and transferring the gold film to the substrate. The particle size of the No. 1 nano-gold particles is 20nm to 35nm; the particle size of the No. 2 nano-gold particles is 150nm to 200nm. The present invention utilizes two nano-gold particles with significant differences in particle size scales for co-assembly to obtain more surface enhanced Raman scattering hot spots, improve the sensitivity of the co-assembled nano-array surface enhanced Raman scattering substrate, and the adsorption of malachite green on the surface of the co-assembled nano-array surface enhanced Raman scattering substrate realizes surface enhanced Raman scattering detection with higher sensitivity of malachite green.

Claims

1. A method for detecting malachite green, characterized in that: The following steps are involved: The co-assembled nanoarray surface enhanced Raman scattering substrate is placed in the malachite green solution to be detected and immersed for 28 minutes to 32 minutes, taken out, dried, and then the surface enhanced Raman scattering signal is detected to obtain the Raman intensity of the malachite green solution to be detected; Substituting the Raman intensity of the malachite green solution to be detected into the standard curve of malachite green solution concentration-Raman intensity to obtain the concentration of the malachite green solution to be detected; the pH value of the malachite green solution to be detected is 3-5; The preparation steps of the co-assembled nanoarray surface enhanced Raman scattering substrate are as follows: Sodium citrate solution and chloroauric acid solution are added into water to carry out reduction reaction, thereby obtaining nano-gold solution No. 1; Mixing a gold seed solution, a sodium citrate solution, a hydroxylamine hydrochloride solution and a chloroauric acid solution to obtain an intermediate, and mixing the intermediate with a reducing agent and a chloroauric acid solution to obtain a No. 2 nanogold solution; Co-assembling the No. 1 nanogold solution and the No. 2 nanogold solution in a water-organic solvent system, assembling the No. 1 nanogold particles into the gaps of the No. 2 nanogold particle array to obtain a gold film, and configuring the gold film on a substrate to obtain a co-assembled nanoarray surface enhanced Raman scattering substrate; The particle size of the No. 1 nano-gold particles is 30 nm; The particle size of the No. 2 nano-gold particles is 190 nm; The mass percentage concentration of the sodium citrate solution is 1%, and the mass percentage concentration of the chloroauric acid solution is 1%; The volume ratio of the No. 1 nanogold solution to the No. 2 nanogold solution is 100:500; In the water-organic solvent system, the organic solvents are dichloromethane and n-hexane; In the water-organic solvent system, the volume ratio of dichloromethane, n-hexane and water is 2:1:0.8~1.

1.

2. The method for detecting malachite green according to claim 1, characterized in that: In the process of preparing No. 1 nanogold, the volume ratio of the sodium citrate solution, the chloroauric acid solution and water is 9-11:1:90-110.

3. The method for detecting malachite green according to claim 2, characterized in that: The specific preparation process of the gold seed solution is as follows: Add sodium citrate solution and chloroauric acid solution into water to obtain a gold seed solution; The volume ratio of the sodium citrate solution, the chloroauric acid solution and water is 1.15-1.17:1:100-102.

4. The method for detecting malachite green according to claim 3, characterized in that: In the process of preparing the No. 2 nanogold solution, the reducing agent is a hydroxylamine hydrochloride solution; The concentration of the hydroxylamine hydrochloride solution is 0.005 mol / L to 0.015 mol / L; The volume ratio of the gold seed solution, the hydroxylamine hydrochloride solution, the chloroauric acid solution and the sodium citrate solution is 30-35:10-20:1:2.

Images