Insect acousto-optic behavior testing device

Abstract

The invention relates to the technical field of insect biological research, in particular to an insect acousto-optic behavior testing device. An insect acousto-optic behavior testing device comprises a box body, a suspension system is used for suspending and fixing an insect to be observed in the center of the device, night vision cameras are arranged on the periphery of the insect to be observed, infrared laser sensors are arranged on the two sides of the insect to be observed, and a rotating disc system is arranged below the insect to be observed. The rotating disc system moves back and forth through the sliding rail and rotates by itself, and is used for adjusting the position of the stimulation system. According to the insect flying and walking behavior testing device provided by the invention, through multi-directional camera shooting recording, adjustable sound pressure and wind speed control, accurate flapping frequency recording, a flexible sound and visual stimulation system, an innovative tracing ball system and reliable experimental environment control, the insect flying and walking behavior testing device can be used for testing the insect flying and walking behavior. The comprehensive, accurate and controllable test of insect flying and walking behaviors is realized, and powerful technical support is provided for insect behavioristics research.

Description

Technical Field

[0001] The invention relates to the technical field of insect biology research, and in particular to an insect sound and light behavior testing device. Background Art

[0002] Studying the flight and walking behaviors of insects under acoustic signals and visual stimulation is of great significance for research in the fields of ecology, agricultural science, and entomology. Insects often communicate and respond through environmental signals such as sound and light during their survival and reproduction. These signals not only affect the mating behavior of insects, but also play a key role in the communication between insects and their natural enemies. Recording the behavioral responses of insects to acoustic and visual stimulation helps to reveal their behavioral ecological characteristics and provide a scientific basis for field pest control strategies.

[0003] Although the current research devices can observe and analyze insect behaviors to a certain extent, they usually have some limitations. For example, the technical solution disclosed in Chinese patent CN215380946U: an insect phototaxis test device based on acoustic sensing, including a phototaxis reaction box, a dark reaction box, an acoustic sensor, and a light source LED, characterized in that the interior of the phototaxis reaction box is a cavity, and a partition is installed in the phototaxis reaction box; the partition is connected to the phototaxis reaction box; the acoustic sensor is installed on the back of the phototaxis reaction box, and the acoustic sensor is connected to the phototaxis reaction box; the light source LED is installed on the partition, and the light source LED is connected to the partition; a dark reaction box is installed at the bottom of the phototaxis reaction box, and the dark reaction box is connected to the phototaxis reaction box. The phototaxis reaction box is divided into two parts: a box body and a baffle; the baffle is installed on the back of the box body, and the baffle is detachably connected to the box body; the baffle is installed on the surface of the baffle, and the baffle is fixedly connected to the baffle; the baffle is provided with multiple. Hinge structures are installed on the left and right sides of the box body; hinge structures are installed on the left and right sides of the partition in the middle of the baffle; one end of the hinge structure is fixedly connected to the box body and the partition respectively. A light source LED is installed on the other end of the hinge structure, and the light source LED is fixedly connected to the hinge structure; the light source LED is adjusted by the hinge structure; reflectors are installed diagonally on the left and right sides of the box body, and the reflectors are fixedly connected to the box body. A dark reaction box is installed at the bottom of the box body of the phototaxis reaction box, and the dark reaction box is connected to the bottom of the box body through the through-connection; the bottom surface of the dark reaction box is an opening, and a movable door is provided on the bottom surface of the dark reaction box; the movable door is rotatably connected to the dark reaction box through a hinge. A sliding groove is provided in the dark reaction box, and a through groove is provided on the side of the dark reaction box; a movable plug plate is installed on the dark reaction box, and the movable plug plate is slidably connected to the dark reaction box through the through groove. A power control box is installed at the bottom of the box body of the phototaxis reaction box, and the interior of the power control box is a cavity; a power module, a drive module, a system control module, a remote control module and a display module are installed in the power control box; the system control module is a microcomputer, and the system control module is wirelessly connected to the sound sensor.

[0004] Although the above device can be used to study the phototaxis of insects, its experimental purpose is relatively single. Current research equipment has problems such as low precision, incomplete data recording, and large interference with insect behavior in terms of spatial flexibility of the stimulus source and capturing insect behavior data, which reduces the accuracy and reliability of the data. Therefore, it is of great scientific value and application prospect to develop a test device that can fully stimulate and accurately record the flight and walking behavior of insects with minimal interference. Summary of the invention

[0005] The purpose of the present invention is to address the defects of the prior art and provide an insect sound and light behavior testing device, which can set sound and light stimulation sources at any spatial point, accurately record the behavior of insects during flight and walking, and adjust the test environment to obtain accurate data with minimal interference.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions: An insect sound and light behavior testing device, characterized in that it comprises a box, wherein the box is provided with a suspension system, a night vision camera, a microphone, an infrared laser sensor, a turntable system, a stimulation system, a tracer ball system, a signal receiving system and a video signal receiving system, and an output system; The suspension system is used to suspend the insect to be observed at the center of the device, the night vision cameras are arranged around the insect to be observed, infrared laser sensors are arranged on both sides of the insect to be observed, the turntable system is arranged below the insect to be observed, the turntable system moves back and forth through the slide rail and rotates itself, and is used to adjust the position of the stimulation system, the stimulation system is arranged on the turntable, and includes a sound stimulation mechanism and a light stimulation mechanism, and the tracer ball system is arranged below the insect to be observed, and is used for the insect to be observed to walk; The signal receiving system collects infrared laser sensor data, tracer ball data and signal data of the stimulation system, and the video signal receiving system is used to collect night vision camera data; The output system processes the camera and microphone data and integrates them into a multi-channel split-screen video, and records the type and start and end time of the stimulus, the insect's body posture, environmental parameters, sound pressure meter readings, and laser sensor data.

[0007] Specifically: 1. Precision tweezers are used as hanging claws to hang small insects in the air, but they are not completely fixed. The small insects can move freely to simulate the normal flight process.

[0008] 2. The tracer ball system is relatively large and heavy, and can be raised and lowered on the bottom surface. The tracer ball system does not work when the small insect is flying, and rises to work when the small insect is walking.

[0009] 3. The video signal receiving and output system is specially designed. Because the application scenario of this test is to shoot tiny insects in a dark environment, a camera with high magnification, night vision, red light filtering, and split-screen output is required. Traditional night vision cameras need to illuminate the object being photographed, and the light spots generated by the strong fill light facing them need to be filtered. Such high-magnification night vision cameras cannot do split-screen output. Therefore, this application integrates the output of the high-magnification night vision camera into a four-split-screen output, which is an innovative solution for this application in a special application scenario.

[0010] 4. The infrared laser sensor is also connected to a three-axis micromanipulator, which enables precise adjustment of the infrared laser sensor. This high-precision control system can ensure that the recorded wingbeat frequency is accurate, thereby improving the accuracy of the data and the repeatability of the experiment.

[0011] Furthermore, there are four night vision cameras, which are respectively arranged above, below, left and right of the insects to be observed. The night vision cameras integrate high-magnification night vision, red light filtering and split-screen output functions, can effectively illuminate in dark environments and avoid light spot interference, and provide clear video images of tiny insects.

[0012] Furthermore, a fan system is embedded in the box, and the fan system is a silent fan.

[0013] Furthermore, the sound stimulation mechanism is a speaker placed on the turntable, and the light stimulation mechanism includes an LED matrix light arranged in the box. The speaker can be a wide-spectrum speaker, an ultrasonic transmitter, etc., which can be replaced according to actual needs.

[0014] Furthermore, the device also includes a three-dimensional movable slide rail, and the infrared laser sensor and the suspension system are respectively fixed on the three-dimensional movable slide rail. Through the combination of the turntable and the slide rail system, the experimental equipment can arbitrarily adjust the position of the sound playback in space, which greatly facilitates the study of the impact of sound on the flight behavior of insects and improves the flexibility and exploratory nature of the experiment. At the same time, the high-precision adjustment of the three-dimensional slide rail system ensures that the relative position and angle between the sensor and the insect can be accurately adjusted, ensuring the accuracy of the experimental data, so as to conduct complex behavior pattern analysis.

[0015] Furthermore, the suspension system includes a clip and tweezers, wherein the clip is fixed on a three-dimensional movable slide rail, one end of the tweezers is fixed to the clip, and the other end is fixed to the insect to be observed.

[0016] Furthermore, the device also includes a retractable sound pressure meter, which is arranged directly below the insect to be observed.

[0017] Furthermore, the device also includes a retractable anemometer, which is arranged directly below the insect to be observed.

[0018] The retractable sound pressure meter and anemometer described above can accurately measure sound and wind speed parameters when needed, ensuring accurate environmental control and data recording.

[0019] Furthermore, the tracer ball system is a sphere held up by airflow, with an optical infrared sensor under the sphere to record the movement and speed of the ball and output data in the form of switch quantity. The tracer ball system monitors the movement and speed of the ball through laser optical infrared sensors, provides accurate records of insect walking behavior, and outputs accurate pulse data, further enriching the research data of insect behavior.

[0020] Furthermore, the box contains a darkroom, and the box is equipped with a temperature and humidity sensor. The integrated design of the external box and environmental control realizes comprehensive control of the experimental environment. The laser sensor, night vision camera and other sensitive components are stabilized by the vibration-proof platform, and the temperature and humidity sensor monitors the environmental data in real time. The micro humidifier automatically adjusts the humidity to ensure the stability of the experimental environment. It is particularly suitable for darkroom experiments that require strict environmental control.

[0021] Among them, the device is remotely connected to a control terminal, such as a computer host connected via a local area network, which can remotely monitor settings to reduce physical interference, and monitor and control experimental equipment located in another space through an external workstation, thereby realizing real-time data transmission and parameter adjustment of experimental equipment, greatly reducing physical interference during the experiment, and improving data accuracy and experimental repeatability.

[0022] By adopting the technical solution of the present invention, the beneficial effects of the present invention are: 1. All-round video data acquisition: It adopts modular design and is equipped with four night vision cameras to achieve all-round recording of insect flight and walking behaviors. By flexibly configuring the placement of the night vision cameras, it can accurately adjust the ventral and dorsal directions of the insects, output an integrated four-split screen video interface, and perfectly adapt to different observation needs. Correspondingly, the laser system and fan system will be optimized and adjusted according to the changes in the position of the night vision camera to ensure the maximum shooting effect during the experiment.

[0023] 2. Diversity and stability of environmental simulation: It has a highly integrated sensor array, combined with various environmental sensors, sound pressure meters and anemometers, to accurately record experimental conditions and adjust internal settings in real time based on data feedback to ensure the optimization of the best experimental environment. Environmental sensors refer to thermometers, hygrometers and air quality sensors. Among them, the silent fan with adjustable wind speed and air volume can simulate different flight environments, thereby enhancing the naturalness and reliability of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a structural schematic diagram of a first embodiment of an insect sound and light behavior testing device provided by the present invention.

[0025] Figure 2 It is a structural schematic diagram of a second embodiment of an insect sound and light behavior testing device provided by the present invention.

[0026] Among them, there are a box body 1, a three-dimensional movable slide rail 2, an infrared laser sensor 3, a suspension system 4, a clip 5, a night vision camera 6, a turntable system 7, a sound stimulation mechanism 8, and a light stimulation mechanism 9. DETAILED DESCRIPTION

[0027] The specific embodiments of the present invention are further described in conjunction with the accompanying drawings.

[0028] Embodiment 1, as Figure 1 As shown, an insect sound and light behavior testing device includes a box 1 to achieve the functions of temperature and humidity control.

[0029] Furthermore, the box 1 is a darkroom, and the box 1 is equipped with a temperature and humidity sensor to monitor the temperature and humidity in real time, and output this information and behavioral data to the computer synchronously. A micro humidifier is installed in the box 1, which automatically humidifies when the humidity is too low. The box 1 can conduct darkroom experiments, monitor environmental data in real time through temperature and humidity sensors, and automatically control the micro humidifier to adjust the humidity.

[0030] Furthermore, the device also includes a three-dimensional movable slide rail 2, and the infrared laser sensor 3 and the suspension system 4 are respectively fixed on the three-dimensional movable slide rail 2, so as to accurately adjust the angle between the infrared laser sensor 3 and the front wings and hind wings of the insect to be observed. The three-dimensional movable slide rail 2 can be adjusted and set according to actual needs, such as Figure 1 As shown, it is only represented by simple lines. It is actually a rod-shaped frame structure. A caliper-like structure is set on the rod-shaped frame to adaptively adjust and fix the rods in multiple directions in three dimensions. Among them, the longitudinally arranged rods can also be circular rods to facilitate the adjustment of the lateral rod angles.

[0031] Furthermore, infrared laser sensors 3 are provided on both sides of the insect to be observed. In this embodiment, two infrared laser sensors are provided to record the wing beat frequency, and the output signal is a switch quantity. Each infrared laser sensor 3 is fixed on a three-axis micromanipulator to accurately adjust the angle between the sensor and the front and rear wings of the insect, wherein the three-axis micromanipulator is an existing product available on the market.

[0032] Furthermore, the suspension system 4 is used to suspend and fix the insect to be observed in the center of the device to ensure its stable position during the test. The suspension system 4 is in the form of tweezers, which can clamp the insect to be observed, and the tweezers are fixed on the three-dimensional movable slide rail 2 through the clamp 5.

[0033] Furthermore, night vision cameras 6 are arranged around the insects to be observed. In this embodiment, four cameras are arranged, which are respectively located above, below, to the left and to the right of the insects to be observed, for all-round photography of the flight behavior of the insects to be observed. The data of the four night vision cameras are integrated through a video signal receiver and then enter the PC for real-time split-screen monitoring, recording and storage.

[0034] Furthermore, the turntable system 7 is arranged below the insect to be observed. The turntable system 7 moves back and forth and rotates itself through the slide rail connected to it in the three-dimensional movable slide rail 2, and is used for adjusting the position of the stimulation system.

[0035] Furthermore, the stimulation system is arranged on the turntable system 7, including the sound stimulation mechanism 8 and the light stimulation mechanism 9. The turntable system 7 has a slide rail that can move in and out, and a slider that can slide up and down and fix the sound stimulation mechanism 8 and the light stimulation mechanism 9 is erected on the slide rail. By remotely controlling the turntable system 7 and the slide rail, the stimulation source can be realized relative to any point in space of the insect to be observed.

[0036] Furthermore, the sound stimulation mechanism 8 is a broad-spectrum speaker placed on the turntable system 7, which can emit sound signals of different frequency ranges. The light stimulation mechanism 9 includes a 5cm*5cm LED matrix light arranged on the turntable, which can emit light of different colors and shapes. The sound signal or LED visual signal is output from the PC into two signals through software design, one enters the speaker or LED light board, and the other enters the analog-to-digital conversion card, which is used to collect various types of data and monitor, record and save them in real time through the PC.

[0037] Furthermore, a tracer ball system is provided under the insect to be observed to record the whereabouts of the insect to be observed. The tracer ball system is a sphere held up by airflow, and the insect to be observed can be fixed on it and walk freely. An optical infrared sensor is provided under the sphere to record the movement and speed of the tracer ball system and output data in the form of switch quantity to ensure accurate recording of the insect's walking behavior.

[0038] Furthermore, the signal receiving system collects infrared laser sensor data, tracer ball data and signal data of the stimulation system. The signal receiver is an analog-to-digital conversion card, which collects data from the left infrared laser sensor, the right infrared laser sensor, tracer ball data, sound, and LED light board signal data, and directly connects to the PC for real-time monitoring, recording and storage through software. The video signal receiving system is used to collect night vision camera data. The data of four night vision cameras are connected to the video signal receiver, and the four video signals are integrated together and connected to the PC for real-time split-screen monitoring, recording and storage. The sound signal is designed using software and is divided into two signals after entering the signal separator. One signal enters the speaker to play the sound, and the other signal is connected to the analog-to-digital conversion card.

[0039] Furthermore, a fan system is installed on the box body, and the fan system is a silent fan with adjustable wind speed and air volume for precise control of airflow.

[0040] Furthermore, the device also includes a retractable sound pressure meter, which is embedded in the box and is an existing product on the market.

[0041] Furthermore, the device also includes a retractable anemometer embedded in the box, and the anemometer is an existing product available on the market.

[0042] The specific observation method of this embodiment is: the insect to be observed is hung in the center of the device, a liftable tracer ball system is placed below, and its flight and walking behaviors are recorded in real time through four night vision cameras 6. The silent fan 12 in the front can adjust the wind speed and air volume to provide different flight environments for the insect to be observed. The infrared laser sensor 3 records the wingbeat frequency of the insect to be observed in real time, and adjusts the sensor angle through a three-axis micromanipulator to ensure the accuracy of the data. The turntable system 7 and the three-dimensional movable slide rail 2 below allow the speaker to move in three-dimensional space, and by playing sounds and visual signals at different positions, the influence of sound on the flight and walking behaviors of insects is studied. The retractable sound pressure meter is used to measure the intensity of the sound signal to ensure the accuracy of the sound stimulation experiment; the retractable anemometer is used to measure the wind speed and air volume in real time to ensure the accuracy of the environmental simulation.

[0043] In the second embodiment, infrared laser sensors 3 are provided on both sides of the insect to be observed. In this embodiment, three infrared laser sensors 3 are provided to record the wing beat frequency, and the output signal is a switch quantity. Each infrared laser sensor 3 is fixed on a three-axis micromanipulator to accurately adjust the angle between the sensor and the front and rear wings of the insect, wherein the three-axis micromanipulator is an existing product available on the market. The rest is the same as in the first embodiment.

[0044] Note that the above are only preferred embodiments of the present invention and the technical principles used. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and that various obvious changes, readjustments and substitutions can be made by those skilled in the art without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in more detail through the above embodiments, the present invention is not limited to the above embodiments, and may also include more other equivalent embodiments without departing from the concept of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. An insect sound and light behavior testing device, characterized in that: It includes a box body, in which a suspension system, a night vision camera, a microphone, an infrared laser sensor, a turntable system, a stimulation system, a tracer ball system, a signal receiving system and a video signal receiving system, and an output system are arranged; The suspension system is used to suspend the insect to be observed at the center of the device, the night vision cameras are arranged around the insect to be observed, infrared laser sensors are arranged on both sides of the insect to be observed, the turntable system is arranged below the insect to be observed, the turntable system moves back and forth through the slide rail and rotates itself, and is used to adjust the position of the stimulation system, the stimulation system is arranged on the turntable, and includes a sound stimulation mechanism and a light stimulation mechanism, and the tracer ball system is arranged below the insect to be observed, and is used for the insect to be observed to walk; The signal receiving system collects infrared laser sensor data, tracer ball data and signal data of the stimulation system, and the video signal receiving system is used to collect night vision camera data; The output system processes the camera and microphone data and integrates them into a multi-channel split-screen video, and records the type and start and end time of the stimulus, the insect's body posture, environmental parameters, sound pressure meter readings, and laser sensor data.

2. The insect sound and light behavior testing device according to claim 1, characterized in that: There are four night vision cameras, which are respectively arranged above, below, left and right of the insect to be observed. The night vision cameras integrate high-magnification night vision, red light filtering and split-screen output functions, can effectively illuminate in dark environments and avoid light spot interference, and provide clear video images of tiny insects.

3. The insect sound and light behavior testing device according to claim 1, characterized in that: A fan system is embedded in the box, and the fan system is a silent fan.

4. The insect sound and light behavior testing device according to claim 1, characterized in that: The sound stimulation mechanism is a loudspeaker placed on the turntable, and the light stimulation mechanism includes an LED matrix lamp arranged in a box.

5. The insect sound and light behavior testing device according to claim 1, characterized in that: The device also includes a three-dimensional movable slide rail, and the infrared laser sensor and the suspension system are respectively fixed on the three-dimensional movable slide rail.

6. The insect sound and light behavior testing device according to claim 5, characterized in that: The suspension system comprises a clip and tweezers, wherein the clip is fixed on a three-dimensional movable slide rail, one end of the tweezers is fixed to the clip, and the other end is fixed to the insect to be observed.

7. The insect sound and light behavior testing device according to claim 1, characterized in that: The device also includes a retractable sound pressure meter, which is arranged directly below the insect to be observed.

8. The insect sound and light behavior testing device according to claim 1, characterized in that: The device also includes a retractable anemometer, which is arranged directly below the insect to be observed.

9. The insect sound and light behavior testing device according to claim 1, characterized in that: The tracer ball system is a sphere held up by air flow, with an optical infrared sensor under the sphere to record the movement and speed of the ball and output the data in the form of switch quantity.

10. The insect sound and light behavior testing device according to claim 1, characterized in that: The box body is a darkroom, and the box body is provided with temperature and humidity sensors.

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