Application of a compound ROT300 in resisting tobacco mosaic virus in plants

Abstract

The invention relates to the technical field of agricultural drugs, and discloses an application of a compound ROT300 in resisting plant tobacco mosaic virus. The compound ROT300 is N-N'-bis(2,6-diisopropylphenyl)-1,6,7,12-tetraphenoxy-3,4,9,10-perylenetetracarboxylic acid diimide, and its chemical formula is C 72 H 58 N 2 O 8 . The compound can be used in the preparation of anti-plant tobacco mosaic virus drugs. The plant virus is tobacco mosaic virus (TMV), the drug is a plant tobacco mosaic virus inhibitor, and the active ingredient of the plant tobacco mosaic virus inhibitor includes ROT300. When performing plant antiviral operations, distilled water is used to dissolve the ROT300 compound into a working concentration of 100‑500 µg / mL, and 0.5% DMSO is added to promote dissolution, and the inhibition rate of TMV reaches more than 80%. After the tobacco was treated with ROT300, the growth state of the tobacco was good, without obvious inhibition or poor growth, indicating that ROT300 is safe for the host plant and has no phytotoxic effect.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural drugs, and in particular to application of a compound ROT300 in resisting plant tobacco mosaic virus. Background Art

[0002] Plant viral diseases are a global challenge. Their high frequency of occurrence, rapid spread, difficulty in control, and significant negative impact on crop yield and quality have made this issue a focus of attention. Under environmental conditions such as high temperature and drought that are conducive to the reproduction and spread of viruses, the risk of outbreaks of plant viral diseases increases significantly. These environmental factors not only promote the replication and spread of viruses, but may also weaken the immunity of plants, making them more vulnerable to viruses. Plant viruses are transmitted through a variety of pathways, including insect vectors, soil, seeds, and pollen, which makes prevention and control work particularly complex and difficult. Every year, the economic losses caused by plant viral diseases can reach tens of billions of euros worldwide, seriously affecting agricultural production and the development of the national economy.

[0003] Currently, there are relatively few types of control agents for plant viral diseases on the market, which cannot meet the diverse control needs. The control effects of some agents are not ideal, the agents are not highly targeted or the viruses have developed drug resistance, especially when facing newly emerging viruses or mutant viruses. Based on this, the present application proposes the use of a compound ROT300 in resistance to plant tobacco mosaic virus to solve the above problems. Summary of the invention

[0004] 1. Technical issues to be solved

[0005] In view of the deficiencies of the prior art, the present invention provides an application of a compound ROT300 in resisting tobacco mosaic virus. The compound of the present invention has a novel structure and low toxicity, and can be used as a high-grade pigment for coloring plastics. After the compound ROT300 is used to treat tobacco, the tobacco grows well without obvious inhibition or poor growth, indicating that ROT300 is safe for host plants and has no phytotoxic effect.

[0006] (II) Technical solution

[0007] To achieve the above object, the present invention provides an application of a compound ROT300 in resisting tobacco mosaic virus in plants, wherein the compound ROT300 is N-N'-bis(2,6-diisopropylphenyl)-1,6,7,12-tetraphenoxy-3,4,9,10-perylenetetracarboxylic acid diimide, and its chemical formula is C 72 H 58N2O8. The compound can be used in the preparation of an anti-plant tobacco mosaic virus drug, wherein the plant virus is tobacco mosaic virus (TMV), the drug is a plant tobacco mosaic virus inhibitor, and the active ingredient of the plant tobacco mosaic virus inhibitor includes ROT300. When performing plant anti-virus operations, the ROT300 compound is dissolved in distilled water to a working concentration of 100-500 μg / mL, and 0.5% DMSO is added to promote dissolution, and then incubated with the plant virus to inhibit the virus; or first applied to the leaf surface of a healthy plant to protect the plant from being infected by the plant virus; or applied to leaves infected with the virus to inhibit the virus.

[0008] (III) Beneficial effects

[0009] Compared with the prior art, the present invention provides a compound for inhibiting plant tobacco mosaic virus, which has the following beneficial effects:

[0010] The anti-plant virus activity of ROT300 was evaluated by the half-leaf spot method. The results showed that ROT300 effectively inhibited the proliferation of tobacco mosaic virus. The inactivation activity of 100 and 500 μg / mL of ROT300 against tobacco mosaic virus reached 68.4% and 87.2%, respectively. The protective activity of 100 and 500 μg / mL of ROT300 against tobacco mosaic virus reached 39.1% and 81.3%, respectively. The inactivation activity of 100 and 500 μg / mL of ROT300 against tobacco mosaic virus reached 43.1% and 48.6%, respectively. In addition, after treatment, the tobacco grew well without obvious inhibition or poor growth, indicating that ROT300 is safe for host plants and has no phytotoxic effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 is the molecular formula of the compound ROT300 of the present invention;

[0012] Figure 2 This is a schematic diagram of the inactivation activity of the compound ROT300 of the present invention in inhibiting tobacco mosaic virus TMV;

[0013] Figure 3 This is a schematic diagram of the protective activity of the compound ROT300 of the present invention in inhibiting tobacco mosaic virus TMV;

[0014] Figure 4 This is a schematic diagram of the therapeutic activity of the compound ROT300 of the present invention in inhibiting tobacco mosaic virus TMV;

[0015] Figure 5 This is a schematic diagram showing the inhibition of the compound ROT300 of the present invention on tobacco mosaic virus TMV. DETAILED DESCRIPTION

[0016] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] See also Figure 1-5 The present invention provides a compound ROT300, which is N-N'-bis(2,6-diisopropylphenyl)-1,6,7,12-tetraphenoxy-3,4,9,10-perylenetetracarboxylic acid diimide, and its chemical formula is C 72 H 58 N2O8. The compound can be used in the preparation of anti-plant tobacco mosaic virus drugs, the plant virus is one or more of tobacco mosaic virus (TMV), potato virus Y and cucumber mosaic virus, specifically, the drug is a plant tobacco mosaic virus inhibitor, the active ingredient of the plant tobacco mosaic virus inhibitor includes ROT300, when performing plant antiviral operation, use distilled water to dissolve the ROT300 compound into a working concentration of 100-500 μg / mL, add 0.5% DMSO to promote dissolution, and then apply it on leaves infected with the virus.

[0018] In order to demonstrate the effect of ROT300 in resisting plant viruses, the present invention evaluates the anti-plant virus activity of ROT300 by the half-leaf spot method. The application test process of ROT300 in inhibiting plant viruses is as follows:

[0019] 1) Dissolve the ROT300 compound in distilled water to a working concentration of 100 and 500 μg / mL;

[0020] 2) Incubate the virus: Add TMV extract (TMV final concentration is 0.05 mg / mL) to the ROT300 aqueous solution, mix gently, incubate on ice for 30 min, and add the same concentration of TMV extract to the water as the control group;

[0021] 3) Frictional poisoning: ① Use 6-7 leaf stage Sansi tobacco as test tobacco, add appropriate amount of quartz sand to the leaves of Sansi tobacco, use gauze swab to apply 100μL of ROT300 and TMV mixed solution to the left half of the tobacco leaf along the main vein of the leaf, and apply 100μL TMV mixed solution to the right half of the leaf as the control group. This method is to test the inhibitory activity of ROT300 on TMV;

[0022] ② Apply ROT300 compound solution at different concentrations on one side of healthy tobacco leaves, and apply 0.5% DMSO aqueous solution on the other side as a blank. After 24 hours of incubation, apply equal amounts of TMV extract on both sides of the tobacco leaves. This method is to test the protective activity of ROT300 in inhibiting TMV;

[0023] ③ The ROT300 compound solution was applied to one side of the tobacco leaves infected with TMV at different concentrations, and the other side contained 0.5% DMSO aqueous solution as a blank control. This method was used to test the therapeutic activity of ROT300 in inhibiting TMV;

[0024] 4) The inhibition rate of ROT300 compound on TMV was calculated by the number of leaf lesions (the calculation formula is: inhibition rate (%) = [(CT) / C] × 100%, where "C" represents the average number of lesions in the control half leaf and "T" represents the average number of lesions in the half leaf treated with ROT300).

[0025] The experimental results are as follows:

[0026] The test results show that the anti-plant virus activity of ROT300 was evaluated by the half-leaf spot method. The results show that ROT300 effectively inhibits the proliferation of TMV. The inactivation activity of ROT300 at 100 and 500 μg / mL to inhibit tobacco mosaic virus reached 68.4% and 87.2%, respectively. Figure 2 and Figure 5 ; The protective activity of ROT300 against tobacco mosaic virus at 100 and 500 μg / mL reached 39.1% and 81.3%, respectively. Figure 3 and Figure 5 ; 100 and 500 μg / mL of ROT300 showed 43.1% and 48.6% inhibitory activity against tobacco mosaic virus, respectively. Figure 4 and Figure 5 After ROT300 was applied to tobacco, the growth of Sansi tobacco was good, with no obvious inhibition or poor growth, indicating that ROT300 was safe to host plants and had no phytotoxic effects, indicating that ROT300 had the potential to be developed into a plant virus inhibitor.

[0027] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An application of a compound ROT300 in resisting tobacco mosaic virus in plants, characterized in that: The compound ROT300 is N-N'-bis(2,6-diisopropylphenyl)-1,6,7,12-tetraphenoxy-3,4,9,10-perylenetetracarboxylic acid diimide, and its chemical formula is C 72 H 58 N2O8.

2. The use of a compound ROT300 according to claim 1 in resisting tobacco mosaic virus in plants, characterized in that: The plant virus is tobacco mosaic virus (TMV).

3. The use of a compound ROT300 according to claim 2 in resisting tobacco mosaic virus in plants, characterized in that: The compound ROT300 is used in preparing drugs for resisting plant tobacco mosaic virus.

4. The use of a compound ROT300 according to claim 3 in resisting tobacco mosaic virus in plants, characterized in that: The drug is a plant tobacco mosaic virus inhibitor.

5. A method for plant antiviral treatment using the compound ROT300 according to any one of claims 1 to 4, characterized in that: ROT300 compounds were dissolved in distilled water to a working concentration of 100-500 µg / mL, and 0.5% DMSO was added for dissolution, and then applied to leaves infected with tobacco mosaic virus.

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