Insecticide composition and its application

Abstract

The present invention belongs to the field of pesticides and insecticides and relates to an insecticidal composition. The insecticidal composition comprises active ingredients A and B, wherein active ingredient A is a compound of formula (I) and active ingredient B is any one of flupyroxystrobin, indazapyroxamet, and nicofluprole. The mass ratio of active ingredient A to active ingredient B is 1:50 to 34:1. The insecticidal composition of the present invention has the advantages of enhancing synergy and delaying resistance, and is used to control resistant pests.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides and insecticides, and particularly relates to an insecticide composition and application thereof. Background Art

[0002] Flupyroxystrobin is a methoxyacrylate insecticide with high activity against the dipteran pests fruit flies, leafminers, leafminers, and bradya fungimids, as well as thrips. Its chemical name is (2E)-3-methoxy-2-(2-{[4-(trifluoromethyl)pyridin-2-yl]oxy}phenyl)prop-2-enoic acid methyl ester, and its chemical formula is:

[0003]

[0004] Indazapyroxamet is a pyridine-containing amide insecticide developed by FMC. It is used to control Thysanoptera or Hemiptera pests such as western flower thrips, potato leafhoppers, aphids, and whiteflies. Its chemical name is: N-(1-methylcyclopropyl)-2-(3-pyridyl)-2H-benzopyrazole-4-carboxamide, and its chemical formula is:

[0005]

[0006] Nicofluprole is a nicotinamide compound with broad-spectrum insecticidal activity, showing excellent insecticidal activity against Lepidoptera pests, Hemiptera pests, and Acarina pests. Its chemical name is 2-chloro-N-cyclopropyl-5-(1-(2,6-dichloro-4-(perfluoropropane-2-yl)phenyl)-1H-pyrazol-4-yl)-N-methylnicotinamide, and its chemical formula is:

[0007]

[0008] In agricultural production, the long-term, continuous, high-dose use of a single chemical pesticide or pesticide with a single mode of action can easily lead to problems such as pest resistance and the evolution of resistance. Rational compounding or mixing of pesticides offers advantages such as reduced pesticide usage, improved control effectiveness, and delayed development and progression of pest resistance, making it one of the most effective approaches to addressing these issues. Mixing the compound of formula (I) with flupyroxystrobin, indazapyroxamet, and nicofluprole can achieve rapid and simultaneous control of different pests, broadening the control spectrum, improving pesticide efficacy, and mitigating the development of pest resistance. Currently, no reports on this topic have been found. Summary of the Invention

[0009] The present invention aims to provide an insecticide composition which has synergistic effects, reduces resistance and has low use cost.

[0010] To achieve the above object, the technical solution adopted by the present invention is: an insecticide composition comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I) with the following structural formula:

[0011] The active ingredient B is any one of Flupyroxystrobin, Indazapyroxamet, and Nicofluprole;

[0012] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 34:1;

[0013] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:24 to 28:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:27 to 27:1, such as 1:27, 1:18, 1:9, 2:3, 3:2, 9:2, 18:1, 27:1 or any value therebetween;

[0015] Furthermore, the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:18 to 27:1;

[0016] Furthermore, the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:18, 1:9, 2:3, 3:2, 9:2, 18:1, and 27:1;

[0017] Furthermore, the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:50 to 30:1, such as 1:50, 1:32, 1:22, 1:16, 1:11, 1:10, 1:6, 2:11, 2:5, 5:2, 10:3, 11:2, 17:1, 18:1, 24:1, 30:1 or any value therebetween;

[0018] Furthermore, the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:32 to 24:1;

[0019] Furthermore, the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:32, 1:22, 1:16, 1:11, 1:10, 1:6, 2:11, 2:5, 5:2, 10:3, 11:2, 17:1, 18:1, and 24:1;

[0020] Furthermore, the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 34:1, such as 1:24, 1:14, 1:8, 2:3, 7:1, 14:3, 28:1, 34:1 or any value therebetween;

[0021] Furthermore, the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 28:1;

[0022] Furthermore, the mass ratio of the compound of formula (I) to Nicofluprole is 1:24, 1:14, 1:8, 2:3, 7:1, 14:3, and 28:1;

[0023] Furthermore, based on the total mass of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 0.5% to 90%;

[0024] Furthermore, based on the total mass of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 1% to 85%;

[0025] Furthermore, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 1% to 85%;

[0026] Furthermore, the insecticide composition further comprises, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients, wherein the auxiliary ingredients are selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0027] Furthermore, the insecticide composition further comprises an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist and a carrier;

[0028] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0029] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0030] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0031] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0032] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0033] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0034] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0035] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0036] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0037] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0038] Synergists are selected from synergist, piperonyl butoxide; and / or

[0039] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0040] Furthermore, the insecticide composition can be prepared into an agriculturally acceptable formulation, wherein the formulation is selected from a solid formulation and / or a liquid formulation;

[0041] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0042] Furthermore, the liquid preparation includes a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a fat, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion;

[0043] Furthermore, the solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions;

[0044] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from emulsifiable concentrates and suspension concentrates;

[0045] Application of the insecticide composition of the present invention in preventing and controlling agricultural and forestry pests and sanitary pests;

[0046] The insecticide composition of the present invention can be used to control pests on fruit trees, vegetables, ornamental plants, tea, cotton, and cereal crops;

[0047] Furthermore, the agricultural and forestry pests and sanitary pests are Lepidoptera and Thysanoptera pests;

[0048] Furthermore, the Lepidoptera pests include: Adoxophyes spp., Adoxophyes orana, Agrotis spp. (cutworms), Agrotis ipsilon (black cutworm), Alabama argillacea (cotton leafworm), Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anacamptodes defectaria, Anarsia lineatella (peach twig borer), Anomis sabulifera (jute looper), Anticarsia g emma talis, Archips argyrospila (fruittree leafroller), Archips rose leafroller, and A. rosana (rose leaf roller), Argyrotaenia spp. (tortricid moths), Argyrotaenia citrana (orange tortrix), Autographa gamma, Bonagota cranaodes, Borbo cinnara (rice leaf folder), Bucculatrix thurberiella (cotton leaf perforator), Caloptilia spp. (leaf miners), Capua reticulana, Carposina niponensis (peach fruit moth), Chilo spp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded rose leaf roller), leafroller)), Chrysodeixis spp.), Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus (carpenter moth), Crambus spp. (sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella (codling moth), Darna diducta, Diaphania spp. (stem borer), borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugarcane borer), Diatraea graniosella (southwester corn borer), Earias spp. (cotton bollworm), Earias insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopopha aurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Epiphysias postruttana (light brown apple moth), Ephestia spp. (flour moths), Ephestia cautella)(almond moth), tobacco pink moth.

[0049] Ephestia elutella (tobbaco moth), Ephestiakuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella (grape berry moth), Euxoa auxiliaris (army cutworm), Feltia spp. (cutworms), Gortyna spp. (stemblerers), Grapholita molesta (oriental fruit moth), Hedylepta indicate (bean leaf roller), leafwebber), Helicoverpa sp. (spotworm), Helicoverpa paarmigera, Helicoverpa zea, Heliothis spp., Heliothis virescens, Hellula undalis (cabbage webworm), Indarbela spp. (root borers), Keiferia lycopersicella, Leucinodes orbonalis, Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruitmoth), Loxagrotis spp.), Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp. (tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis (bean pod borer), Metisa plana, Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis, Nymphula depunctalis, Operophtherabrumata, Ostrinia nubilalis (European corn borer), Oxydia vesulia, Pandemis cerasana (common currant tortrix), Pandemis heparana (brown apple tortrix), Papilio demodocus, Pectinophora gossypiella (pink bollworm), Peridroma spp. (cutworms), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp.), Pieris rapae, Plathypena scabra, Plodia interpunctella, Plutellaxylostella (diamondback moth), Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays oleae (olive moth), Pseudaletia spp. (moths), Spodoptera litura Fabricius, Pseudaletia unipunctata (armyworm), Pseudoplusia includes (, Rachiplusia nu), Chilo suppressalis (Walker), Scirpophaga incertulas, Sesamia spp. (stem borer). spp.) (stemborers), Sesamia inferens (pink rice stem borer), Spodoptera frugiperda (JESmmith), Sesamia nonagrioides, Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp., Spodoptera exigua, Spodoptera fugiperda, Spodoptera oridania (southern armyworm), Synanthedon spp.), Thecla basilides, Thermisia gemmatalis, Tineola bisselliella (webbing clothesmoth), Trichoplusia ni (cabbage worm), Pierisrapae Linne (cabbage worm), Tuta absoluta (tomato leafminer);

[0050] Further, the Thysanoptera pests include: Thrips palmiKarny, Thrips tabaci, Frankliniella fusca (tobacco thrips), Frankliniella occidentalis, (western flower thrips), Frankliniella shultzei, Frankliniella williamsi (cornthrips), Heliothrips haemorrhaidalis, greenhouse thrips, Riphiphorothrips cruentatus, Scirtothrips spp., Scirtothrips citri (citrus thrips), Scirtothrips spp. dorsalis) (yellow tea thrips), Taeniothrips rhopalantennalis, and Thrips spp.;

[0051] Furthermore, the lepidopteran pests are cabbage worm, diamondback moth, beet armyworm, Spodoptera litura, Spodoptera litura, Chilo suppressalis, and cotton bollworm;

[0052] Furthermore, the Thysanoptera pests are western flower thrips, tobacco thrips, palm thrips, and watermelon thrips;

[0053] The present invention also provides a method for using the insecticide composition as described above, specifically applying an effective dose to the pests to be controlled or the medium in which they grow.

[0054] The insecticide composition of the present invention has the following beneficial effects:

[0055] 1) The insecticide composition of the present invention exhibits a synergistic effect within a certain ratio range, and has a significant control effect;

[0056] 2) The insecticide composition of the present invention can delay the development of insecticide resistance, is environmentally friendly, can reduce the amount of pesticides used, and lower the cost of pest control. DETAILED DESCRIPTION

[0057] The present invention is further described below with reference to the examples. The percentages in the examples are all by weight, but the present invention is not limited thereto.

[0058] The composition of the present invention can be provided in the form of a formulation. It can be formulated as a suspension concentrate, emulsifiable concentrate, water-dispersible granules, aqueous emulsion, granules, wettable powder, or dispersible oil suspension, as needed. The content of the active ingredient in the composition of the present invention depends on the dosage when used alone, as well as the blending ratio and the degree of synergistic effect. The optimal range of the active ingredient content varies depending on the formulation type of the composition.

[0059] Preparation Example

[0060] Example 1:

[0061] 25% formula (I) compound flupyroxystrobin suspension (15+10)

[0062] Formula: 15% compound of formula (I), 10% flupyroxystrobin,

[0063] 4% fatty alcohol polyoxyethylene ether phosphate, 2% sodium lignin sulfonate, 2.5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% magnesium aluminum silicate, 0.2% xanthan gum, 0.5% methyl paraben, 5% ethylene glycol, 0.5% silicone defoamer, and deionized water to make up the balance;

[0064] Preparation method: Add active ingredients to the wetting dispersant and defoamer, use zirconium oxide beads, and wet grind with a sand mill to D 90 (90% of the particles have a particle size of <10 μm) to obtain a pulverized slurry. A thickener, antifreeze agent, preservative, etc. are added to the pulverized slurry and mixed evenly. Deionized water is added to 100% and high-speed shearing is performed to obtain a suspension preparation of the composition of the present invention.

[0065] Example 2:

[0066] 28% compound of formula (I)·indazapyroxamet suspension (8+20)

[0067] Formula: 8% compound of formula (I), 20% indazapyroxamet,

[0068] 2% sodium lignin sulfonate, 2.5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 4% alkylphenol polyoxyethylene ether phosphate, 1% magnesium aluminum silicate, 0.2% xanthan gum, 0.5% methyl paraben, 5% ethylene glycol, 0.5% silicone defoamer, and deionized water to make up the balance;

[0069] Preparation method: Same as Example 1.

[0070] Example 3:

[0071] 20% compound of formula (I)·nicofluprole suspension (8+12)

[0072] Formula: 8% compound of formula (I), 12% nicofluprole,

[0073] 4.2% styrylphenol ethoxylate phosphate, 2.5% sodium lignin sulfonate, 2.5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% magnesium aluminum silicate, 0.3% xanthan gum, 0.5% sodium sorbate, 5% ethylene glycol, 0.5% silicone defoamer, and deionized water to make up the balance;

[0074] Preparation method: Same as Example 1.

[0075] Example 4:

[0076] 45% formula (I) compound flupyroxystrobin water dispersible granules (25+20)

[0077] Formula: 25% compound of formula (I), 20% flupyroxystrobin,

[0078] 2% sodium lignin sulfonate, 6% sodium polycarboxylate, 5% naphthalene sulfonate formaldehyde condensate, 3.5% sodium lauryl sulfate, 5% white carbon black, 2% bentonite, and kaolin to make up the balance;

[0079] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow grinding, appropriate water is added, and then kneading, granulation, drying, and sieving can be made into a water-dispersible granule preparation of the composition of the present invention.

[0080] Example 5:

[0081] 50% compound of formula (I)·indazapyroxamet water dispersible granules (37.5+22.5)

[0082] Formula: 37.5% compound of formula (I), 22.5% indazapyroxamet,

[0083] 6% sodium polycarboxylate, 2.5% sodium lignin sulfonate, 6% naphthalene sulfonate formaldehyde condensate, 3.5% sodium lauryl sulfate, 3% ammonium sulfate, 4% white carbon black, 2% bentonite, and kaolin makes up the balance;

[0084] Preparation method: same as Example 4.

[0085] Example 6:

[0086] 1.4% compound of formula (I)·indazapyroxamet aqueous emulsion (1+0.4)

[0087] Formula: 1% compound of formula (I), 0.4% indazapyroxamet,

[0088] 12% trimethylbenzene, 10% cyclohexanone, 1% calcium dodecylbenzene sulfonate, 1% sodium fatty alcohol polyoxyethylene ether sulfate, 6.5% EO / PO block copolymer, 5% glycerol, 1.5% glycerol, 0.1% silicone defoamer, 0.2% xanthan gum, 0.1% sodium benzoate, and deionized water to make up the balance;

[0089] Preparation method: According to the formula ratio of the embodiment, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase. Deionized water and antifreeze are mixed together to form a uniform water phase; under high-speed shear, the water phase is added to the oil phase to form a well-dispersed water emulsion preparation.

[0090] Example 7:

[0091] 5.2% compound of formula (I)·nicofluprole aqueous emulsion (3.2+2)

[0092] Formula: 3.2% compound of formula (I), 2% nicofluprole,

[0093] 15% cyclohexanone, 12% trimethylbenzene, 1% styrylphenol ethoxylate phosphate, 6% EO / PO block copolymer, 5% glycerol, 2% glycerol, 0.1% silicone defoamer, 0.1% xanthan gum, 0.1% sodium benzoate, and deionized water to make up the balance;

[0094] Preparation method: same as Example 6.

[0095] Example 8:

[0096] 20% compound of formula (I)·nicofluprole dispersible oil suspension (8+12)

[0097] Formula: 8% compound of formula (I), 12% nicofluprole,

[0098] 2.5% fatty alcohol polyoxyethylene ether, 15% castor oil polyoxyethylene ether, 0.3% organic bentonite, 0.2% organosilicon defoamer, 2% succinate sulfonate, and soybean oil to make up the balance;

[0099] Preparation method: According to the formula ratio, the active ingredient, surfactant and other functional additives are placed in a reaction kettle in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand grinding, and finally homogenized filtration to prepare a dispersible oil suspension preparation of the composition of the present invention.

[0100] Indoor biological activity assay

[0101] Refer to agricultural industry standards:

[0102] Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 6: Insect Dipping Method (NY / T 1154.6-2006);

[0103] Guidelines for Indoor Bioassay Tests of Pesticides—Insecticides Part 14: Leaf Dip Method (NY / T 1154.14-2008);

[0104] Guidelines for Indoor Bioassay Tests of Pesticides - Pesticides Part 7: Determination of Combined Action of Mixtures (NY / T1154.7-2006), etc.

[0105] Specific indoor toxicity test:

[0106] 1) Lepidoptera larvae: a combination of leaf and insect dipping;

[0107] Test insect age: Use sensitive populations raised indoors for multiple generations, and select healthy and consistent second-instar Lepidoptera larvae.

[0108] Use tweezers to place fresh cabbage slices in the drug solution and immerse them for 10 seconds, then take them out. After the drug solution dries naturally, place the cabbage slices in a culture dish lined with moisturizing filter paper. Place 16 second-instar lepidopteran larvae in the drug solution for 5 seconds, absorb the excess drug solution with filter paper, and place the test insects in a culture dish with leaves soaked in the corresponding concentration.

[0109] Each treatment was repeated 4 times, with a blank treatment as a control. The treated insects were reared in an artificial intelligence culture chamber at 26±1°C, a light intensity of L:D=16h:8h, and a relative humidity of 60%.

[0110] Investigation method: After the pesticide treatment, the mortality of the test insects was investigated 48 hours later. The criterion for judging the death of the test insects was obvious shrinkage of the insect body or inability to crawl normally after being pricked with a needle. The number of dead insects was recorded.

[0111] 2) Thysanoptera adults: glass roller tube method;

[0112] Test insect age: Use sensitive populations raised indoors for multiple generations, and select healthy and consistent female adults of the order Thysanoptera.

[0113] After the glass tube is evenly rolled with 250ul of the drug solution, the cabbage leaves are punched into 1.5cm diameter discs with a hole punch. The fresh cabbage slices are placed in the drug solution with tweezers and immersed for 10s before being taken out. After the drug solution is naturally dried, the cabbage slices are placed in the glass tube. 20 female adults of Thysanoptera are sucked into the glass tube with an insect sucker and the tube is sealed with a 200-mesh gauze.

[0114] Each treatment was repeated 4 times, with a blank treatment as a control. The treated insects were reared in an artificial intelligence culture chamber at 26±1°C, a light intensity of L:D=16h:8h, and a relative humidity of 60%.

[0115] Investigation method: After the treatment with the pesticide, the death of the test insects was investigated 48 hours later. The criterion for judging the death of the test insects was that they could not crawl normally after being pricked with a needle. The number of dead insects was recorded.

[0116] Data statistics and analysis:

[0117] Based on the survey data, calculate the adjusted mortality rate of each treatment using the following formula, with the results rounded to two decimal places:

[0118]

[0119] Where:

[0120] P——mortality rate, in percentage (%);

[0121] K——indicates the number of dead insects, the unit is head;

[0122] N——represents the total number of insects processed, in heads.

[0123]

[0124] Where:

[0125] P1——adjusted mortality rate, in percentage (%);

[0126] P t ——Treatment mortality rate, expressed in percentage (%);

[0127] P0 - blank control mortality rate, in percentage (%).

[0128] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0129] The data were processed by probability value analysis. The DPS statistical analysis system was used to analyze the toxicity regression line and LC 50The activity of the test agents on the biological materials was evaluated by using the values, 95% confidence limits and correlation coefficient r.

[0130] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0131]

[0132] Where:

[0133] ATI - measured toxicity index of mixture;

[0134] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0135] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0136] TTI=TI A *P A +TI B *P B

[0137] Where:

[0138] TTI – Theoretical Toxicity Index of Mixtures;

[0139] TI A ——Agent toxicity index;

[0140] P A ——The percentage of agent A in the mixture, in percentage (%);

[0141] TI B ——Toxicity index of agent B;

[0142] P B ——The percentage of agent B in the mixture, in percentage (%).

[0143]

[0144] Where:

[0145] CTC – Co-toxicity coefficient;

[0146] ATI - measured toxicity index of mixture;

[0147] TTI - Theoretical Toxicity Index of Mixture.

[0148] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0149] Test results:

[0150] Combined toxicity of mixed pesticides to Pieris rapae:

[0151] The results in Tables 1 and 2 show that the mixture of the compound of formula (I) with Indazapyroxamet and Nicofluprole has a good combined effect on Pieris rapae, and exhibits an obvious synergistic effect at a certain mass ratio.

[0152] When the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:32 to 30:1, the co-toxicity coefficient of the control effect against Pieris rapae is greater than 80, showing an additive or synergistic effect; when the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:32 to 18:1, the co-toxicity coefficient of the control effect against Pieris rapae is greater than 120, showing a synergistic effect.

[0153] Table 1 Toxicity test results of different ratios of compound of formula (I) and Indazapyroxamet against Pieris rapae

[0154]

[0155] When the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 34:1, the co-toxicity coefficient of the control effect against Pieris rapae is greater than 80, showing an additive or synergistic effect; when the mass ratio of the compound of formula (I) to Nicofluprole is 1:24 to 28:1, the co-toxicity coefficient of the control effect against Pieris rapae is greater than 120, showing a synergistic effect.

[0156] Table 2 Toxicity test results of different ratios of compound of formula (I) and Nicofluprole to Pieris rapae

[0157]

[0158]

[0159] Combined toxicity of mixed pesticides to Thrips tabaci:

[0160] The results in Tables 3 and 4 show that the compound of formula (I) mixed with Flupyroxystrobin and Indazapyroxamet has a good combined effect on Thrips tabaci, and exhibits obvious synergistic effect at a certain mass ratio.

[0161] When the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:27 to 27:1, the co-toxicity coefficient of the control effect on Thrips tabaci is greater than 80, showing an additive or synergistic effect; when the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:18 to 27:1, the co-toxicity coefficient of the control effect on Thrips tabaci is greater than 120, showing a synergistic effect.

[0162] Table 3 Toxicity test results of different ratios of compound of formula (I) and Flupyroxystrobin against Thrips tabaci

[0163]

[0164] When the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:50 to 24:1, the co-toxicity coefficient of the control effect on Thrips tabaci is greater than 80, showing an additive or synergistic effect; when the mass ratio of the compound of formula (I) to Indazapyroxamet is 1:22 to 24:1, the co-toxicity coefficient of the control effect on Thrips tabaci is greater than 120, showing a synergistic effect.

[0165] Table 4 Toxicity test results of different ratios of compound of formula (I) and Indazapyroxamet against Thrips tabaci

[0166]

[0167]

[0168] Field efficacy examples

[0169] Field trial 1

[0170] Experimental crops: Chinese chives (Dajingou);

[0171] Conditions of the experimental field: medium fertility, convenient irrigation and drainage, and sandy soil.

[0172] Test target: leek thrips;

[0173] Experimental location: Qiantianbutou Village, Haiyang City, Yantai City;

[0174] Trial date: November 8, 2022;

[0175] Application method: In the test field, a knapsack electric sprayer was used to apply pesticides manually according to the designed plan. The whole plant was sprayed first, and then the back of the leaves was sprayed with emphasis. The application machine was a 3WBS-16 knapsack electric sprayer with a spray volume of 30 L / mu.

[0176] Experimental environment: All experimental plots were uniformly cultivated, with the same fertilizer and water management level, good water and fertilizer conditions, and clay loam soil;

[0177] Experimental drug:

[0178] Table 5 Field test design and dosage

[0179]

[0180] Test method: Apply the pesticide once at the early stage of leek thrips infestation (small white spots appear on the leaves).

[0181] Plot size and repetition: random block arrangement, plot size 25m 2 , each treatment was repeated 4 times.

[0182] Survey method: Randomly sample five points in each plot and use the clapping method. At each point, randomly survey 5 clumps (20 plants) together and clap them 3 times. Count the number of thrips shaken into the white porcelain plate. Survey the base number of insects before spraying, and survey the number of remaining insects 1 day and 7 days after spraying.

[0183] Calculation formula and data analysis:

[0184]

[0185] Field efficacy test results:

[0186] The field control effects of each agent on leek thrips are shown in Table 6. It can be seen from the table that 1 day after application, 25% formula (Ⅰ) compound·flupyroxystrobin suspension concentrate (15+10) and 28% formula (Ⅰ) compound·indazapyroxamet suspension concentrate (8+20) showed good fast-acting properties against leek thrips, and the active ingredient dosage was 10g ai / hm 2 The control effects were 87.35% and 86.19% respectively. With the increase of time, 7 days after application, the control effects of 25% formula (Ⅰ) compound·flupyroxystrobin suspension (15+10) and 28% formula (Ⅰ) compound·indazapyroxamet suspension (8+20) on leek thrips increased. The dosage of active ingredient was 10g ai / hm 2 The protective effects were 92.27% and 91.44% respectively, which were significantly higher than the control single-dose and lasted longer.

[0187] Table 6 Field control effects of different treatment agents on leek thrips

[0188]

[0189] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0190] Field trial 2

[0191] Test crops: Chinese cabbage (Green Show);

[0192] Test target: Pieris rapae;

[0193] Experimental location: Chinese cabbage planting experimental field in Lijia Village, Zhucheng, Weifang City, Shandong Province;

[0194] Trial date: October 6, 2022;

[0195] Application method: Use a knapsack electric sprayer in the test field to apply pesticides manually according to the designed plan. First spray the whole plant, and then focus on spraying the back of the leaves.

[0196] Experimental environment: The terrain is flat, the soil is loamy, and the fertility is medium. The cultivation conditions in all experimental plots are uniform and consistent, and the same fertilizer and water management level is adopted. The water and fertilizer conditions are relatively good, and the soil is clay loam;

[0197] Experimental drug:

[0198] Table 7 Field test design and dosage

[0199]

[0200] Test method: Apply the pesticide once during the peak period of cabbage looper occurrence (mostly 2nd to 4th instar).

[0201] Plot size and repetition: random block arrangement, plot size 20m 2 , each treatment was repeated 4 times.

[0202] Survey method: Randomly sample five points in each plot, hang a sign on 4 cabbage plants at each point, investigate the number of cabbage loopers on all cabbages, investigate the base number of insects before spraying, and investigate the number of remaining insects 3 days and 7 days after spraying.

[0203] Calculation formula and data analysis:

[0204]

[0205] Field efficacy test results: The field control effects of various agents on cabbage loopers are shown in Table 8. It can be seen from the table that 3 days after application, 1.4% formula (I) compound·indazapyroxamet emulsion (1+0.4) and 20% formula (I) compound·nicofluprole dispersible oil suspension (8+12) showed good fast-acting properties against cabbage loopers, and the active ingredient dosage was 15 g a.i / hm 2 The control effects were 84.17% and 85.28% respectively. With the increase of time, 7 days after application, the control effects of 1.4% formula (Ⅰ) compound·indazapyroxamet emulsion (1+0.4) and 20% formula (Ⅰ) compound·nicofluprole dispersible oil suspension (8+12) on Pieris rapae increased. The dosage of active ingredient was 15g ai / hm 2 The protective effects at different times were 89.63% and 90.20% respectively, which were significantly higher than the control single-dose and lasted longer.

[0206] Table 8 Field control effects of different treatment agents on cabbage worms

[0207]

[0208] Note: The above insect population base and control efficacy data are the average of 4 replicates, and the values ​​are rounded to two decimal places. Different letters after the control efficacy data in the same column indicate significant differences (P < 0.05).

[0209] Safety: During the trial period and subsequent observations, the Chinese cabbage grew normally and no obvious pesticide damage was observed.

[0210] The insecticide composition or formulation obtained by compounding the present invention exhibits significant control efficacy, outperforming single agents in delaying the development of insecticide resistance and prolonging insecticide retention. Furthermore, no crop damage was observed with the compounded formulation in tests, demonstrating that the insecticide composition or formulation, while enhancing synergistic insecticide efficacy, can reduce production and use costs and is safe for crops.

[0211] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. An insecticidal composition, characterized in that The invention comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), The structural formula is as follows: (I); The active ingredient B is Flupyroxystrobin, and the mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:18-27:

1.

2. The insecticidal composition according to claim 1, wherein: The mass ratio of the compound of formula (I) to Flupyroxystrobin is 1:18, 1:9, 2:3, 3:2, 9:2, 18:1, and 27:

1.

3. The insecticidal composition according to claim 1, wherein: Based on the total mass of the insecticide composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the insecticide composition is 0.5% to 90%.

4. The insecticidal composition according to claim 1, wherein: In addition to the active ingredients, the insecticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

5. The insecticidal composition according to claim 4, characterized in that: The insecticide composition is prepared into an agriculturally acceptable formulation, wherein the formulation is selected from solid formulations and / or liquid formulations; the solid formulation is selected from wettable powders and water-dispersible granules; and the liquid formulation is selected from emulsifiable concentrates, emulsions in water, microemulsions, suspensions, suspoemulsions, and dispersible oil suspensions.

6. Use of the insecticide composition according to any one of claims 1 to 5 in preventing and controlling agricultural and forestry pests and sanitary pests.

7. The use according to claim 6, characterized in that: The agricultural and forestry pests and sanitary pests are Thysanoptera pests, and the Thysanoptera pests are western flower thrips, tobacco thrips, palm thrips and watermelon thrips.

8. The use according to claim 6, characterized in that: The insecticide composition is applied to the pests to be controlled or the medium where the pests grow at an effective dose.

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