A skin care composition with repairing and anti-aging effects

Abstract

The present invention belongs to the technical field of cosmetics, and specifically relates to a skin care composition with repairing and anti-aging effects, wherein the skin care composition comprises the following components in parts by weight: 2.5-4 parts of common wheat extract, 0.1-1 parts of phospholipids, 1-2 parts of squalane, 2.5-4 parts of caprylic / capric triglycerides, 0.5-1.5 parts of glycolipids, 0.01-0.5 parts of phytosphingosine, 0.001-0.1 parts of ceramide NS / ceramide NG, 0.05-3 parts of gentian root extract, 0.1-2 parts of sea fennel extract, 0.01-0.5 parts of milo leaf / stem extract, 0.01-1.0 parts of rose extract, and 0.01-0.8 parts of schizonepeta extract. The skin care composition of the present invention has repairing and anti-aging effects, and can be used to prepare sebum biomimetic cosmetics.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a skin care composition with repairing and anti-aging effects. Background Art

[0002] The sebum film, also known as the natural moisturizing film, is a transparent film covering the surface of the skin. It is formed by the mixture of sebum secreted by the skin (secreted by the sebaceous glands) and sweat (secreted by the sweat glands) to form a weak acidic protective film on the skin surface, which protects the skin. The amount of sebum secreted by the sebaceous glands of middle-aged and elderly people will decrease, and its composition will also change. The barrier function of the sebum film will weaken, which is prone to dry skin, itching, redness and other uncomfortable symptoms. Dry skin is also due to the low amount of sebum secretion and the weak barrier function of the sebum film. The skin is relatively sensitive and easily stimulated by the outside world.

[0003] Currently, a large number of sebum compositions are used in the field of cosmetics. Cosmetics that simulate sebum ingredients can help replenish lipids on the surface of the skin and enhance the skin's ability to retain water, thereby improving the problems of dryness and roughness. However, there are also some problems with sebum compositions in the field of cosmetics: 1. Stability: Some sebum ingredients may oxidize during storage, resulting in a decline in product quality. 2. Absorbability: It is also a challenge to ensure that the ingredients in the formula can be effectively absorbed by the skin. If the ingredients are too greasy, it may cause clogged pores. 3. Allergic reactions: Even if the formula is as close to human sebum as possible, it may still cause allergic reactions in some individuals.

[0004] In order to improve the stability of the sebum composition, the prior art simultaneously adds Sphingomonas fermentation product extract and fermented fruit oil to improve the stability of the product. However, in actual applications, it is found that the simultaneous addition of these two substances increases allergic reactions, which is not conducive to consumer use.

[0005] Therefore, there is an urgent need for a skin care composition with repairing and anti-aging effects. Summary of the invention

[0006] The object of the present invention is to provide a skin care composition having repairing and anti-aging effects.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] A skin care composition with repairing and anti-aging effects, comprising the following components in parts by weight: 2.5-4 parts of common wheat extract, 0.1-1 parts of phospholipids, 1-2 parts of squalane, 2.5-4 parts of caprylic / capric triglycerides, 0.5-1.5 parts of glycolipids, 0.01-0.5 parts of phytosphingosine, 0.001-0.1 parts of ceramide NS / ceramide NG, 0.05-3 parts of gentian root extract, 0.1-2 parts of sea fennel extract, 0.01-0.5 parts of milo leaf / stem extract, 0.01-1.0 parts of rose extract and 0.01-0.8 parts of schizonepeta tenuifolia extract;

[0009] The preparation method of the common wheat extract comprises the following steps:

[0010] (1) Grinding common wheat into a size smaller than 20-50 mesh to obtain wheat flour, adding water to the wheat flour to obtain a mixture, adding cellulase, pectinase and neutral protease, performing enzymolysis at 30-35° C. for 5-8 hours, inactivating the enzymes, adding Lactobacillus brevis, performing anaerobically fermenting at 30-35° C. for 2-3 days, adding Lactobacillus plantarum, performing anaerobically fermenting at 30-35° C. for 1-2 days, adding Lactobacillus bulgaricus, performing anaerobically fermenting at 30-35° C. for 1-2 days, sterilizing, and vacuum drying to a moisture content of 2-5% to obtain a wheat flour fermentation product;

[0011] (2) Mixing the wheat flour fermentation product with an ethanol aqueous solution having a volume concentration of 60-70%, placing the mixture in a microwave reactor, extracting the mixture at a microwave power of 200-250 W and 30-35° C. for 1-2 h to obtain a crude wheat extract;

[0012] (3) The crude wheat extract is loaded onto a macroporous resin column and eluted with solvent I to obtain eluent A, then eluted with solvent II to obtain eluent B, and finally eluted with solvent III to obtain eluent C. After removing the solvents from eluents A, B and C respectively, the eluents are mixed and dried to obtain a common wheat extract.

[0013] Common wheat extract contains a variety of ingredients that are beneficial to the skin. These ingredients can simulate or support the natural sebum membrane function of the skin to a certain extent. When mixed with other natural ingredients, the anti-aging effect of the skin care composition can be improved, and its function and the characteristics of simulating the sebum membrane can be enhanced. However, when commercially available common wheat extract is added to the system of the present invention, it may react with the ingredients in other components to produce irritating or allergic ingredients, causing certain irritation to the skin. The common wheat extract prepared by enzymatic hydrolysis and fermentation in the present invention is added to the system of the present invention, and after mixing with other components, the irritation of the skin care composition can be improved. The analysis shows that the ingredients are milder through enzymatic hydrolysis and degradation treatment, and when mixed with other skin care ingredients, the irritation to the skin is reduced.

[0014] Further, the weight ratio of the Myrtlesia japonica leaf / stem extract, the rose extract and the Spinach spatholobi extract is (0.1-0.5):1:(0.4-0.8).

[0015] In the system of the present invention, when the milano leaf / stem extract, rose extract and schisandra extract are used in a specific ratio, the repair effect of the skin care composition can be improved. Analysis shows that the milano extract contains a variety of moisturizing ingredients that can help the skin retain moisture; the milano extract can also promote the regeneration of skin cells and help repair damaged skin barriers, thereby indirectly increasing the water content of the skin. The rose extract contains a variety of natural moisturizing factors, such as amino acids, sugars, etc., which can help the skin lock in moisture and reduce moisture evaporation. The schisandra extract may promote local blood circulation, thereby improving the microcirculation of the skin, which is beneficial to the nutrient supply of skin cells and indirectly increasing the water content of the skin; at the same time, the active ingredients of the three plant extracts can complement each other and work together on different levels of the skin. Some ingredients may help other ingredients to better penetrate into the deep layers of the skin and improve the absorption efficiency of skin care products. The appropriate ratio can ensure that each extract can exert its maximum effect, thereby achieving a better overall effect.

[0016] Furthermore, the weight ratio of the common wheat extract, the gentian root extract, and the sea fennel extract is (2.6-3.0):1:(1.3-1.7).

[0017] After adding the Milo leaf / stem extract, rose extract and Globenia extract to the system of the present invention, the content of the ingredients will change greatly over time, and the use effect will decrease. When the common wheat extract, gentian root extract and sea fennel extract are used in a specific ratio, the stability of the effective ingredients of the entire system can be maintained, the skin barrier can be repaired more lastingly, and the anti-aging and repair effects can be improved. The analysis shows that when the common wheat extract, gentian root extract and sea fennel extract are used in a specific ratio, the microenvironment of the skin care composition can be changed, the activity of the active components that cause the decomposition of other ingredients can be inhibited, and the stability of other ingredients can be improved.

[0018] Furthermore, in the step (1), the amount of cellulase in the mixture is 100 U / mL-120 U / mL.

[0019] Furthermore, in step (1), the amount of pectinase in the mixture is 60U / mL-80U / mL.

[0020] Furthermore, in the step (1), the amount of the neutral protease in the mixture is 140 U / mL-160 U / mL.

[0021] Furthermore, in step (1), the concentration of Lactobacillus brevis in the mixture is 10 10-10 11 CFU / mL.

[0022] Furthermore, in step (1), the concentration of Lactobacillus plantarum in the mixture is 10 8 -10 9 CFU / mL.

[0023] Furthermore, in step (1), the concentration of Lactobacillus bulgaricus in the mixture is 10 6 -10 7 CFU / mL.

[0024] Furthermore, in the step (3), the solvent I is an ethanol aqueous solution with a volume concentration of 20-30%; the solvent II is an ethanol aqueous solution with a volume concentration of 40-50%; and the solvent III is an ethanol aqueous solution with a volume concentration of 60-70%.

[0025] Furthermore, the preparation method of the skin care composition comprises the following steps: mixing the components to obtain.

[0026] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0027] 1. The present invention mixes the common wheat extract with other natural ingredients to improve the anti-aging effect of the skin care composition. The common wheat extract prepared by enzymatic hydrolysis and fermentation is added to the system of the present invention and mixed with other components to improve the irritation of the skin care composition.

[0028] 2. In the system of the present invention, when the Myrtillus chinensis leaf / stem extract, the rose extract and the Spinach extract are used in a specific ratio, the repair effect of the skin care composition can be improved.

[0029] 3. When common wheat extract, gentian root extract and sea fennel extract are used in a specific proportion, the stability of the effective ingredients of the entire system can be maintained. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] The raw materials used in the following examples of the present invention are all commercially available products:

[0032] Lecithin was purchased from Anqing Zhongchuang Engineering Technology Co., Ltd., transparent lecithin, general lecithin TY.

[0033] Squalane, Xi'an Zhongkeda Biotechnology Co., Ltd., squalane with product number ZKD1279.

[0034] Caprylic / capric triglyceride, Guangzhou Youyu New Materials Co., Ltd., relative density 0.920-0.960g / cm 3 .

[0035] Glycolipids, Ceramela, Toyobo Co., Ltd.

[0036] Phytosphingosine, Aladdin, product number D136389.

[0037] Ceramide NS / Ceramide NG, Shanghai Shunfeng Chemical Technology Co., Ltd.

[0038] Gentiana root extract, from Xinyang Mufan Biotechnology Co., Ltd., product number MF-006135, specification 5%.

[0039] Sea fennel extract, Gansu Yishengxiang Biotechnology Co., Ltd., specification 10:1 ratio extraction.

[0040] Preparation of Milo leaf / stem extract: Coarsely grind Milo and pass it through a 20-mesh sieve; take 20g of Milo and dissolve it in 500mL of deionized water, heat it to 90℃, stir and extract it for 1h, and cool it to room temperature; after coarse filtration through a 100-mesh filter, centrifuge it at 5000r / min for 5min, take the supernatant, finely filter the supernatant through 0.8μm pore size cardboard, and dry it to obtain the Milo leaf / stem extract.

[0041] Rose extract, Shanyang Lianfeng Biotechnology Co., Ltd., specification 10:1 ratio extraction.

[0042] Globenia extract, Gansu Yishengxiang Biotechnology Co., Ltd., specification 10:1 ratio extraction.

[0043] Lactobacillus bulgaricus, number: CCTCCCB20082295, was purchased from China Center for Type Culture Collection.

[0044] Lactobacillus brevis was purchased from Shanghai Collection of Microorganisms, with the number: SHBCCD14346.

[0045] Lactobacillus plantarum was purchased from Shanghai Collection of Microorganisms with the number: SHBCC D16929.

[0046] Lactobacillus casei was purchased from Shanghai Collection of Microorganisms Center, with the number: SHBCCD24737.

[0047] Lactobacillus helveticus was purchased from China Center for Type Culture Collection, with the number: CCTCCAB2010205.

[0048] Bifidobacterium lactis was purchased from Shanghai Microbiological Collection Center, with the number: SHBCCD24728.

[0049] Pectinase, cellulase, neutral protease, ficin, β-glucanase, and bromelain were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0050] Example 1

[0051] The present embodiment provides a skin care composition with repairing and anti-aging effects, which comprises the following components in parts by weight: 2.8 parts of common wheat extract, 0.4 parts of phospholipids, 1.3 parts of squalane, 3.2 parts of caprylic / capric triglycerides, 1 part of glycolipids, 0.13 parts of phytosphingosine, 0.05 parts of ceramide NS / ceramide NG, 1 part of gentian root extract, 1.5 parts of sea fennel extract, 0.34 parts of mirabilis leaf / stem extract, 1 part of rose extract and 0.5 parts of angelica extract.

[0052] The preparation method of the common wheat extract comprises the following steps:

[0053] (1) Common wheat was crushed into less than 30 meshes to obtain wheat flour, and water 10 times the weight of the wheat flour was added to the wheat flour to obtain a mixture, and cellulase, pectinase and neutral protease were added, and the amount of pectinase in the mixture was 70 U / mL; the amount of cellulase in the mixture was 110 U / mL; the amount of neutral protease in the mixture was 150 U / mL; enzymolysis was performed at 32°C for 6 h, and after the enzyme was inactivated, Lactobacillus brevis was continued to be added, and the concentration of Lactobacillus brevis in the mixture was 10 10 CFU / mL; anaerobic fermentation at 32℃ for 2 days, and then Lactobacillus plantarum was added. The concentration of Lactobacillus plantarum in the mixture was 10 8 CFU / mL; anaerobic fermentation at 32℃ for 1 day, and then Lactobacillus bulgaricus was added. The concentration of Lactobacillus bulgaricus in the mixture was 10 7 CFU / mL; anaerobic fermentation at 32°C for 2 days, sterilization, vacuum drying to a moisture content of 3%, and obtaining wheat flour fermentation product;

[0054] (2) Mix the wheat flour fermentation product with a volume ratio of 1:5 and an ethanol aqueous solution with a volume concentration of 65%, put it into a microwave reactor, extract it at a microwave power of 230 W and 32°C for 1.5 h to obtain a crude wheat extract;

[0055] (3) The crude wheat extract is loaded onto a macroporous resin column, first eluted with solvent I to obtain eluent A, then eluted with solvent II to obtain eluent B, and finally eluted with solvent III to obtain eluent C, wherein solvent I is an ethanol aqueous solution with a volume concentration of 25%; the solvent II is an ethanol aqueous solution with a volume concentration of 45%; and the solvent III is an ethanol aqueous solution with a volume concentration of 65%. After removing the solvents from eluents A, B, and C, the eluents are mixed and dried to obtain a common wheat extract.

[0056] The preparation method of the skin care composition comprises the following steps: mixing the components uniformly to obtain the skin care composition.

[0057] Example 2

[0058] The present embodiment provides a skin care composition with repairing and anti-aging effects, which comprises the following components in parts by weight: 2.6 parts of common wheat extract, 1 part of phospholipids, 1 part of squalane, 4 parts of caprylic / capric triglycerides, 0.5 parts of glycolipids, 0.5 parts of phytosphingosine, 0.001 parts of ceramide NS / ceramide NG, 1 part of gentian root extract, 1.3 parts of sea fennel extract, 0.1 parts of mirabilis leaf / stem extract, 1 part of rose extract and 0.8 parts of angelica extract.

[0059] The preparation method of the common wheat extract comprises the following steps:

[0060] (1) Grind common wheat to less than 50 mesh to obtain wheat flour, add water 10 times the weight of wheat flour to the wheat flour to obtain a mixture, add cellulase, pectinase and neutral protease, the amount of pectinase in the mixture is 80U / mL. The amount of cellulase in the mixture is 100U / mL. The amount of neutral protease in the mixture is 160U / mL. After enzymolysis at 30℃ for 7h and enzyme inactivation, continue to add Lactobacillus brevis, the concentration of Lactobacillus brevis in the mixture is 10 10 CFU / mL. Anaerobic fermentation was carried out at 35℃ for 2 days, and Lactobacillus plantarum was added continuously. The concentration of Lactobacillus plantarum in the mixture was 10 8 CFU / mL. Anaerobic fermentation was carried out at 35℃ for 1 day, and Lactobacillus bulgaricus was added continuously. The concentration of Lactobacillus bulgaricus in the mixture was 10 6 CFU / mL. Anaerobic fermentation at 35°C for 2 days, sterilization, and vacuum drying to a moisture content of 2% to obtain wheat flour fermentation product;

[0061] (2) Mix the wheat flour fermentation product with a volume ratio of 1:5 and an ethanol aqueous solution with a volume concentration of 70%, put it into a microwave reactor, extract it at a microwave power of 250 W and 30°C for 2 h to obtain a crude wheat extract;

[0062] (3) The crude wheat extract is loaded onto a macroporous resin column, and first eluted with solvent I to obtain eluent A, then eluted with solvent II to obtain eluent B, and finally eluted with solvent III to obtain eluent C. Solvent I is an ethanol aqueous solution with a volume concentration of 20%; the solvent II is an ethanol aqueous solution with a volume concentration of 50%; and the solvent III is an ethanol aqueous solution with a volume concentration of 60%. After removing the solvents from eluents A, B, and C, the eluents are mixed and dried to obtain a common wheat extract.

[0063] The preparation method of the skin care composition comprises the following steps: mixing the components uniformly to obtain the skin care composition.

[0064] Comparative Example 1

[0065] The difference between this comparative example and Example 1 is that: cellulase, pectinase and neutral protease are added, and the amount of pectinase in the mixture is 70 U / mL; the amount of cellulase in the mixture is 110 U / mL; the amount of neutral protease in the mixture is 150 U / mL; replaced by: adding ficin, β-glucanase and bromelain, the concentration of ficin in the mixture is 70 U / mL; the concentration of β-glucanase in the mixture is 110 U / mL; the concentration of bromelain in the mixture is 150 U / mL.

[0066] Comparative Example 2

[0067] The difference between this comparative example and Example 1 is that Lactobacillus brevis is continued to be added, and the concentration of Lactobacillus brevis in the mixture is 10 10 CFU / mL. Anaerobic fermentation was carried out at 32℃ for 2 days, and Lactobacillus plantarum was added continuously. The concentration of Lactobacillus plantarum in the mixture was 10 8 CFU / mL; anaerobic fermentation at 32℃ for 1 day, and then Lactobacillus bulgaricus was added. The concentration of Lactobacillus bulgaricus in the mixture was 10 7 CFU / mL; anaerobic fermentation at 32°C for 2 days, replaced by: continue to add Lactobacillus casei, the concentration of Lactobacillus casei in the mixture is 10 10 CFU / mL. Anaerobic fermentation was carried out at 32℃ for 2 days, and Lactobacillus helveticus was added. The concentration of Lactobacillus helveticus in the mixture was 10 8 CFU / mL; anaerobic fermentation at 32℃ for 1 day, and then Bifidobacterium lactis was added. The concentration of Bifidobacterium lactis in the mixture was 10 7 CFU / mL; anaerobic fermentation at 32℃ for 2 days.

[0068] Comparative Example 3

[0069] The difference between this comparative example and Example 1 is that the composition ratio is different.

[0070] A skin care composition with repairing and anti-aging effects, comprising the following components in parts by weight: 1 part of common wheat extract, 1.2 parts of phospholipids, 2.5 parts of squalane, 2 parts of caprylic / capric triglyceride, 2 parts of glycolipids, 1.13 parts of phytosphingosine, 0.05 parts of ceramide NS / ceramide NG, 2.45 parts of gentian root extract, 0.05 parts of sea fennel extract, 1 part of mirabilis leaf / stem extract, 0.34 parts of rose extract and 1.5 parts of spatholobi extract.

[0071] Comparative Example 4

[0072] The difference between this comparative example and Example 1 is that the ratios of the Mironia sibiricum leaf / stem extract, the rose extract and the Spinach striata extract are different.

[0073] A skin care composition with repairing and anti-aging effects, comprising the following components in parts by weight: 2.8 parts of common wheat extract, 0.4 parts of phospholipids, 1.3 parts of squalane, 3.2 parts of caprylic / capric triglycerides, 1 part of glycolipids, 0.13 parts of phytosphingosine, 0.05 parts of ceramide NS / ceramide NG, 1 part of gentian root extract, 1.5 parts of sea fennel extract, 1 part of mirabilis leaf / stem extract, 0.5 parts of rose extract and 0.34 parts of schisandra chinensis extract.

[0074] Comparative Example 5

[0075] The difference between this comparative example and Example 1 is that the ratios of the common wheat extract, the gentian root extract and the critrine extract are different.

[0076] A skin care composition with repairing and anti-aging effects, comprising the following components in parts by weight: 1.5 parts of common wheat extract, 0.4 parts of phospholipids, 1.3 parts of squalane, 3.2 parts of caprylic / capric triglycerides, 1 part of glycolipids, 0.13 parts of phytosphingosine, 0.05 parts of ceramide NS / ceramide NG, 2.8 parts of gentian root extract, 1 part of sea fennel extract, 0.34 parts of mirabilis leaf / stem extract, 1 part of rose extract and 0.5 parts of spatholobi extract.

[0077] Comparative Example 6

[0078] The difference between this comparative example and Example 1 is that the common wheat extract is a commercially available product purchased from Shanghai Shunfeng Chemical Technology Co., Ltd.

[0079] Performance Testing

[0080] The skin care compositions prepared in Examples 1-2 and Comparative Examples 1-6 were subjected to performance tests.

[0081] 1. Human skin patch test

[0082] Test population: Volunteers aged between 20 and 50 were selected and randomly divided into 10 groups, with 20 people in each group;

[0083] Test method: First clean the skin of the test area with physiological saline and wait for 5 minutes to dry; select a qualified patch tester, use a closed patch test method, take 0.5mL of the mixed extract in the patch tester, apply it to the back of the subject with special tape, and ask the subject how it feels; bathing is prohibited during the test; apply the cream sample, and the subject should keep the patch area dry within 48 hours, avoid intense exercise, scratching the patch area, long-term sunlight exposure, etc. After 48 hours, remove the tester and mark it. After 30 minutes, wait until the indentation disappears and make a judgment under sufficient light. Record the results according to the skin reaction grade standard in the "Hygiene Standards for Skin Care Products". The skin adverse reaction grade standard is shown in Table 1 below. If there is any discomfort during the process, stop the test immediately. The results are shown in Table 2.

[0084] Table 1 Grading of skin adverse reactions

[0085] Degree of reaction Rating Skin reactions - 0 Negative reaction ± 1 Suspicious reaction, only slight erythema ＋ 2 Weak positive reaction (erythema reaction), erythema, infiltration, edema, and papules may be present ＋＋ 3 Strong positive reaction (herpes reaction), erythema, infiltration, edema, papules and herpes, the reaction may extend beyond the test area ＋＋＋ 4 Very strong reaction (confluent herpes reaction), obvious erythema, severe infiltration, edema, confluent herpes, reaction beyond the test area

[0086] Table 2 Human skin patch test results

[0087]

[0088] 2. Repair: The skin care compositions prepared in the examples and comparative examples were prepared with PBS solution to prepare sample solutions with a mass percentage concentration of 0.5% of each plant composition, and then tested according to the cell scratch test method described below.

[0089] HaCaT cells in logarithmic growth phase (purchased from Wuhan Pronocell Co., Ltd.) were collected and cultured at a cell density of 6×10 5 Cells were inoculated into 6-well culture plates at 100 μg / well. The plates were cultured in a 37°C, 5% CO2 constant temperature incubator for 24 hours. A 200uL pipette tip was used to scratch "damage" perpendicular to the 6-well plate. The cells were washed 3 times with PBS to remove the scratched cells. A culture medium containing 5% FBS was used as a positive control (PC), and untreated cells were used as a negative control (NC). 2 mL of sample solution was added to the sample group, and the plates were cultured in a 37°C, 5% CO2 constant temperature incubator for 24 hours. Three parallels were set up for each group. Each group of migrated cells was photographed using an inverted microscope, and the image pro plus software was used to calculate the average scratch area. The cell migration rate calculation formula for each group is as follows:

[0090] Cell migration rate = (initial scratch area - final scratch area) / initial scratch area × 100%.

[0091] The results are shown in Table 3.

[0092] Table 3 Repair effect test results

[0093] Cell migration rate% Example 1 73.2 Example 2 70.6 Comparative Example 1 63.1 Comparative Example 2 62.8 Comparative Example 3 42.5 Comparative Example 4 63.3 Comparative Example 5 63.7 Comparative Example 6 42.4

[0094] 3. Anti-aging test: Take 5 mg of DPPH (1,1-diphenyl-2-picrylhydrazyl free radical) and dissolve it in 100 ml of solvent (anhydrous ethanol), dissolve it by ultrasonic for 5 minutes, and mix it thoroughly. Take 5 ml of the DPPH solution and check the absorbance A value at 519 nm. The A value is best between 1.2-1.3, which is the DPPH standard solution. Prepare the skin care composition into 0.5 mg / ml sample solution with anhydrous ethanol, take 5 ml of the sample solution and add it to 5 ml of the DPPH standard solution and mix it evenly. Put it in a dark room at room temperature to react for 0.5 hours, take 7.5 ml of the sample solution and add it to 10 ml of anhydrous ethanol solution and mix it evenly, and test the absorbance value at 519 nm.

[0095] DPPH free radical scavenging rate (%) = 1-(A2 / A1) × 100%; A1: absorbance value of a mixed solution consisting of a DPPH solution and anhydrous ethanol; A2: absorbance value of a mixed solution consisting of a DPPH solution and an ethanol solution, and the sample solutions of the embodiment and the comparative example. The results are shown in Table 4.

[0096] Table 4 DPPH free radical scavenging rate test results

[0097] DPPH free radical scavenging rate% Example 1 93.5 Example 2 92.7 Comparative Example 1 89.1 Comparative Example 2 87.8 Comparative Example 3 84.6 Comparative Example 4 85.7 Comparative Example 5 86.4 Comparative Example 6 87.2

[0098] 4. Stability

[0099] The skin care compositions prepared in Examples 1-2 and Comparative Examples 1-6 were sealed and stored at room temperature 25° C. for 90 days, and then subjected to repair and anti-aging tests using the same methods as above. The results are shown in Tables 5 and 6.

[0100] Table 5 Repair effect test results after storage

[0101] Cell migration rate% Example 1 72.1 Example 2 68.8 Comparative Example 1 58.2 Comparative Example 2 57.4 Comparative Example 3 33.7 Comparative Example 4 56.6 Comparative Example 5 56.5 Comparative Example 6 34.1

[0102] Table 6 DPPH free radical scavenging rate test results after storage

[0103] DPPH free radical scavenging rate% Example 1 92.5 Example 2 91.9 Comparative Example 1 83.7 Comparative Example 2 83.8 Comparative Example 3 80.4 Comparative Example 4 83.2 Comparative Example 5 82.1 Comparative Example 6 81.3

[0104] It can be seen from the above performance test results that the skin care composition of Examples 1-2 has excellent repair and anti-aging effects, which is mainly due to the synergistic effect between the components.

[0105] However, the comparative examples are significantly inferior to the embodiments in the corresponding performance tests because they do not adopt the necessary technical solutions. The enzyme types used in comparative example 1 are different, and the fungus types used in comparative example 2 are different, so the repair effect is reduced, and the irritation of the product is increased. In comparative example 3, the ratio of the components of the skin care composition is changed, and it can be seen that the repair and anti-aging effects are reduced, and the product is also irritating. It can be seen that the components of the present invention synergistically exert anti-allergic effects. In comparative example 4, the ratios of the Milo leaf / stem extract, the rose extract, and the Globenia extract are different, and the repair effect of the skin care composition is reduced. In comparative example 5, the ratios of the common wheat extract, the gentian root extract, and the sea fennel extract are different, and the stability of the skin care composition is reduced. In comparative example 6, the common wheat extract is a commercially available product, and the irritation of the skin care composition is increased. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effects.

[0106] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A skin care composition with repairing and anti-aging effects, characterized in that: The skin care composition comprises the following components in parts by weight: 2.5-4 parts of common wheat extract, 0.1-1 parts of phospholipids, 1-2 parts of squalane, 2.5-4 parts of caprylic / capric triglycerides, 0.5-1.5 parts of glycolipids, 0.01-0.5 parts of phytosphingosine, 0.001-0.1 parts of ceramide NS / ceramide NG, 0.05-3 parts of gentian root extract, 0.1-2 parts of sea fennel extract, 0.01-0.5 parts of milo leaf / stem extract, 0.01-1.0 parts of rose extract and 0.01-0.8 parts of schizonepeta tenuifolia extract; The preparation method of the common wheat extract comprises the following steps: (1) Grinding common wheat into a size smaller than 30 meshes to obtain wheat flour, adding water to the wheat flour to obtain a mixture, adding cellulase, pectinase and neutral protease, performing enzymolysis at 30-35°C for 5-8 hours, inactivating the enzymes, adding Lactobacillus brevis, performing anaerobically fermenting at 30-35°C for 2-3 days, adding Lactobacillus plantarum, performing anaerobically fermenting at 30-35°C for 1-2 days, adding Lactobacillus bulgaricus, performing anaerobically fermenting at 30-35°C for 1-2 days, sterilizing, and vacuum drying to a moisture content of 2-5% to obtain a fermented wheat flour; (2) Mixing the wheat flour fermentation product with an ethanol aqueous solution having a volume concentration of 60-70%, placing the mixture in a microwave reactor, extracting the mixture at a microwave power of 200-250 W and 30-35° C. for 1-2 h to obtain a crude wheat extract; (3) The crude wheat extract is loaded onto a macroporous resin column, first eluted with solvent I to obtain eluent A, then eluted with solvent II to obtain eluent B, and finally eluted with solvent III to obtain eluent C. After removing the solvent from eluent A, eluent B and eluent C, the eluents are mixed and dried to obtain a common wheat extract; In the step (3), the solvent I is an ethanol aqueous solution with a volume concentration of 20-30%; the solvent II is an ethanol aqueous solution with a volume concentration of 40-50%; and the solvent III is an ethanol aqueous solution with a volume concentration of 60-70%.

2. The skin care composition with repairing and anti-aging effects according to claim 1, characterized in that: The weight ratio of the Mironia leaf / stem extract, the Rosa rugosa extract and the Spinach extract is (0.1-0.5):1:(0.4-0.8).

3. The skin care composition with repairing and anti-aging effects according to claim 2, characterized in that: The weight ratio of common wheat extract, gentian root extract and sea fennel extract is (2.6-3.0):1:(1.3-1.7).

4. The skin care composition with repairing and anti-aging effects according to claim 1, characterized in that: In the step (1), the amount of cellulase used in the mixture is 100 U / mL-120 U / mL.

5. The skin care composition with repairing and anti-aging effects according to claim 1, characterized in that: In the step (1), the amount of pectinase in the mixture is 60U / mL-80U / mL.

6. The skin care composition with repairing and anti-aging effects according to claim 1, characterized in that: In the step (1), the amount of the neutral protease in the mixture is 140 U / mL-160 U / mL.

7. The skin care composition with repairing and anti-aging effects according to claim 1, characterized in that: In the step (1), the concentration of Lactobacillus brevis in the mixture is 10 10 -10 11 CFU / mL.

8. The skin care composition with repairing and anti-aging effects according to claim 1, characterized in that: In the step (1), the concentration of Lactobacillus plantarum in the mixture is 10 8 -10 9 CFU / mL.

9. The skin care composition with repairing and anti-aging effects according to claim 1, characterized in that: In step (1), the concentration of Lactobacillus bulgaricus in the mixture is 10 6 -10 7 CFU / mL.

10. The skin care composition with repairing and anti-aging effects according to claim 1, characterized in that: The preparation method of the skin care composition comprises the following steps: mixing the components to obtain the skin care composition.

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