Optical detection device and control method thereof, sample analyzer

Abstract

The application discloses an optical detection device and a control method thereof and a sample analyzer. In the optical detection device, a fluorescence excitation assembly is used to emit an excitation light beam, and a bright / dark field illumination assembly is used to emit an illumination light beam. A bearing table is located on an out-light path of the excitation light beam and the illumination light beam. An objective lens is located on the lower side of the bearing table, is used to perform microscopic imaging on fluorescent particles in a sample to be detected to form a first imaging light beam, is used to perform imaging on the sample to be detected under a bright field to form a second imaging light beam, and is used to perform imaging on the sample to be detected under a dark field to form a third imaging light beam. An imaging receiving assembly is located on an out-light path of the objective lens, and is used to receive the first imaging light beam, the second imaging light beam and the third imaging light beam. The optical detection device can not only perform fluorescence detection on a sample, but also perform bright / dark field detection on the sample. Moreover, the optical detection device has the advantages of simple and compact structure, low cost, clear imaging effect and improved accuracy of sample detection.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical equipment, in particular to an optical detection device, a control method thereof and a sample analyzer. BACKGROUND

[0002] Blood component detection items mainly include red blood cell detection, white blood cell detection and platelet detection, etc., and blood component detection is an important clinical medical index. The optical detection method is commonly used for blood component detection. Different detection items generally select different optical detection devices.

[0003] In the related art, the optical detection device of the sample analyzer generally adopts an infinite micro system for imaging, and the optical component cost is relatively high, and the detection items are single, and it is difficult to switch multiple detection modes. SUMMARY

[0004] The present application provides an optical detection device, a control method thereof and a sample analyzer to solve the technical problem that the optical component cost is relatively high in the prior art, and the detection items are single, and it is difficult to switch multiple detection modes.

[0005] To solve the above technical problems, one technical solution adopted by the present application is to provide an optical detection device, which comprises: a fluorescence excitation assembly and a bright / dark field illumination assembly arranged at intervals, a bearing table, an objective lens and an imaging receiving assembly, the fluorescence excitation assembly is used to emit an excitation light beam, and the bright / dark field illumination assembly is used to emit an illumination light beam; the bearing table is located on the light output path of the excitation light beam and the illumination light beam, and is used to carry a sample to be detected; the objective lens is located on the lower side of the bearing table, and is used to perform microscopic imaging on fluorescent particles in the sample to be detected to form a first imaging light beam, and is used to image the sample to be detected under bright field to form a second imaging light beam, and is used to image the sample to be detected under dark field to form a third imaging light beam; the imaging receiving assembly is located on the light output path of the objective lens, and is used to receive the first imaging light beam, the second imaging light beam and the third imaging light beam.

[0006] Further, the imaging receiving assembly comprises: a light selection piece and a receiver, the receiver is located on the light output path of the light selection piece, and the light selection piece comprises at least two light selection sub-pieces connected in sequence, the at least two light selection sub-pieces are movably arranged to move to between the objective lens and the receiver, and are used to select an imaging mode of the first imaging light beam, the second imaging light beam or the third imaging light beam, and the receiver is used to receive the imaging light beam in the preset mode.

[0007] Further, the at least two light selection sub-pieces comprise a cavity body and at least one optical filter connected in sequence, the cavity body is formed with a hole, the hole is used for the first imaging light beam and / or the second imaging light beam to pass through directly, and the optical filter is used for filtering the first imaging light beam.

[0008] Further, the bright / dark field illumination assembly comprises: an illumination light source configured to emit an illumination light beam; a collimating lens located in an optical path of the illumination light source and configured to collimate the illumination light beam; a light shielding member located in the optical path of the collimating lens and configured to shield a preset range of the illumination light beam; and a first focusing lens located in the optical path of the light shielding member and configured to focus the collimated illumination light beam.

[0009] Further, the bright / dark field illumination assembly comprises: an illumination light source configured to emit an illumination light beam; a collimating lens located in an optical path of the illumination light source and configured to collimate the illumination light beam; and a first focusing lens located in the optical path of the collimating lens and configured to focus the collimated illumination light beam.

[0010] Further, the bright / dark field illumination assembly is movably arranged above the carrier table in a vertical direction, so that the bright / dark field illumination assembly has a first state and a second state, in the first state, the first focusing lens is configured to focus the collimated illumination light beam at the pupil of the objective lens, in the second state, a light shielding member is arranged between the first focusing lens and the collimating lens, the light shielding member is configured to shield a preset range of the illumination light beam, and the first focusing lens is configured to focus the illumination light beam passing through the light shielding member on the sample to be detected.

[0011] Further, the bright / dark field illumination assembly further comprises: a light homogenizing member arranged in the optical path of the collimating lens and configured to homogenize the collimated illumination light beam.

[0012] Further, the optical detection device further comprises: a light splitting assembly located in the optical path of the fluorescence excitation assembly, the light splitting assembly is configured to emit the excitation light beam to the objective lens, the objective lens is configured to collimate the excitation light beam and emit it to the sample to be detected, and the light splitting assembly is further configured to guide the first imaging light beam, the second imaging light beam and the third imaging light beam emitted by the objective lens to the imaging receiving assembly.

[0013] To solve the above technical problems, one technical scheme adopted by the present application is to provide a control method of an optical detection device, which is based on the optical detection device of any of the above embodiments. Specifically, the control method comprises: placing a sample to be detected on a carrier table; emitting an excitation light beam by a fluorescence excitation assembly and emitting an illumination light beam by a bright / dark field illumination assembly, wherein the optical paths of the excitation light beam and the illumination light beam pass through the sample to be detected; performing microscopic imaging of fluorescent particles in the sample to be detected by an objective lens to form a first imaging light beam, imaging the sample to be detected under bright field by the objective lens to form a second imaging light beam, and imaging the sample to be detected under dark field by the objective lens to form a third imaging light beam; and receiving the first imaging light beam, the second imaging light beam and the third imaging light beam by an imaging receiving assembly.

[0014] To solve the above technical problems, one of the technical solutions adopted by the present application is to provide a sample analyzer, which comprises a sample preparation device and the optical detection device of any one of the above embodiments, the sample preparation device is used for preparing a sample to obtain a sample to be detected, and the optical detection device is used for optically detecting the sample to be detected.

[0015] The present application has the following advantages: Different from the prior art, the optical detection device provided by the present application comprises a fluorescent excitation assembly and a bright / dark field illumination assembly arranged at intervals, a bearing table, an objective lens, and an imaging receiving assembly. The sample to be detected on the bearing table can generate fluorescence under the action of excitation light. The objective lens can be used for microscopic imaging of fluorescent particles in the sample to be detected to form a first imaging light beam, and can also be used for imaging the sample to be detected under bright field to form a second imaging light beam, and can also be used for imaging the sample to be detected under dark field to form a third imaging light beam. The imaging receiving assembly is used for receiving the first imaging light beam, the second imaging light beam, and the third imaging light beam to obtain an image formed by the sample to be detected. The optical detection device in the present application has a simple and compact structure and low cost. It can not only detect the sample to be detected by fluorescence, but also detect the sample to be detected by bright / dark field. That is, the optical detection device can detect multiple items, and the optical imaging effect is clear, and the detection reliability is high. BRIEF DESCRIPTION OF DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0017] Figure 1 is a structural schematic diagram of an embodiment of the optical detection device provided by the present application;

[0018] Figure 2 is a structural schematic diagram of another embodiment of the optical detection device provided by the present application;

[0019] Figure 3 is a flowchart of an embodiment of the control method of the optical detection device provided by the present application;

[0020] Figure 4 is a structural schematic diagram of an embodiment of the sample analyzer provided by the present application. DETAILED DESCRIPTION

[0021] In order to make the above objectives, characteristics and advantages of the present application more apparent, a detailed description of the specific embodiments of the present application will be given below with reference to the drawings. It should be understood that the specific embodiments described herein are merely intended to explain the present application, but not to limit the present application. In addition, it should be noted that only the parts related to the present application are shown in the drawings for the convenience of description. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative efforts fall within the scope of the present application.

[0022] The terms "first", "second", and the like in the present application are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "include" and "have" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device including a series of steps or units is not limited to the listed steps or units, but optionally further includes steps or units not listed or optionally further includes other steps or units inherent to the process, method, product or device.

[0023] Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearance of the phrase in various places in the specification does not necessarily all refer to the same embodiment, nor does it necessarily refer to a separate or alternative embodiment. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with each other.

[0024] The present application provides an optical detection device which can be used for both fluorescence detection of a sample to be detected and detection of the sample to be detected under bright / dark field, thereby expanding the detection range of the optical detection device. In addition, the optical detection device provided by the present application has a simple and compact structure, low cost, clear optical imaging effect for the sample to be detected, high reliability and strong practicability.

[0025] Please refer to Figure 1 shown, Figure 1 is a structural schematic diagram of an embodiment of the optical detection device provided by the present application. Specifically, the optical detection device 10 comprises a fluorescence excitation assembly 11, a bright / dark field illumination assembly 12, a bearing table 13, an objective lens 14 and an imaging receiving assembly 15 which are arranged at intervals. Optionally, the optical detection device 10 can further comprise a light splitting assembly 16.

[0026] The carrying table 13 is used for carrying a sample to be detected (not shown in the figure), which can be a blood sample, a sweat sample or a saliva sample after specific processing. In a specific embodiment, when the blood sample is detected by fluorescence, the blood sample can be dyed with acridine orange dye 1:1 for 4 minutes to obtain the sample to be detected, and then 5 microliters of the sample to be detected is sucked by a pipette and dropped on the center of the slide, and then a cover glass is used to press the droplet of the sample to be detected at a small acute angle, so that the droplet is uniformly diffused. After diffusion, the slide is placed on the carrying table 13 for optical detection by the optical detection device 10. In other embodiments, the sample can be processed according to the requirements of the detection item to obtain the sample to be detected, such as dilution by a diluent, and the like, which will not be listed one by one.

[0027] The fluorescence excitation assembly 11 is used for emitting an excitation light beam, and the bright / dark field illumination assembly 12 is used for emitting an illumination light beam. The carrying table 13 is located in the light emitting path of the excitation light beam and the illumination light beam. Specifically, the excitation light beam emitted by the fluorescence excitation assembly 11 can be emitted to the sample to be detected on the carrying table 13, and the fluorescent particles in the sample to be detected can excite a fluorescent light beam under the action of the excitation light beam. The illumination light beam emitted by the bright / dark field illumination assembly 12 is emitted to the sample to be detected on the carrying table 13, and the sample to be detected can transmit the illumination light beam.

[0028] The objective lens 14 is located below the carrying table 13 and is used for microscopic imaging of the fluorescent particles in the sample to be detected to form a first imaging light beam, and is used for imaging the sample to be detected in the bright field to form a second imaging light beam, and is used for imaging the sample to be detected in the dark field to form a third imaging light beam. That is, the objective lens 14 is used for magnifying imaging of the sample to be detected. The objective lens 14 can be used for fluorescence detection, bright / dark field detection, and has a simple and compact structure and low cost.

[0029] The imaging receiving assembly 15 is located in a light emitting path of the objective lens 14 and is used for receiving the first imaging light beam, the second imaging light beam and the third imaging light beam. That is, the imaging receiving assembly 15 is used for receiving the image of the sample to be detected formed by the objective lens 14.

[0030] In this application, a fluorescence detection system can be composed of the fluorescence excitation assembly 11, the objective lens 14 and the imaging receiving assembly 15. The excitation light beam emitted by the fluorescence excitation assembly 11 is emitted to the sample to be detected, the sample to be detected is excited to generate fluorescence, the fluorescent particles are imaged by the objective lens 14 to form a first imaging light beam, and the imaging receiving assembly 15 receives the first imaging light beam to obtain the image of the sample to be detected. Based on the image, the sample to be detected is analyzed by fluorescence detection.

[0031] The bright / dark field detection system can be composed of the bright / dark field illumination assembly 12, the objective lens 14 and the imaging receiving assembly 15. The illumination beam emitted by the bright / dark field illumination assembly 12 exits to the sample to be detected. Under the bright field, the objective lens 14 images the sample to be detected to form a second imaging beam. Under the dark field, the objective lens 14 images the sample to be detected to form a third imaging beam. The bright / dark field detection analysis of the sample to be detected is based on the images.

[0032] Therefore, the optical detection device 10 of the present application integrates the fluorescence detection system and the bright / dark field detection system, which can detect the sample to be detected by fluorescence detection and bright / dark field detection, so that the optical detection device 10 can perform multiple item detection and improve the application range of the optical detection device 10. The fluorescence detection system and the bright / dark field detection system can share the objective lens 14 and the imaging receiving assembly 15 and other components, so that the structure of the optical detection device 10 can be simplified and the cost of the optical detection device 10 can be saved. Moreover, the present application uses a finite distance system for imaging, which has clear imaging effect and can improve the accuracy of the detection of the sample to be detected.

[0033] Further, as shown in Figure 1 The imaging receiving assembly 15 includes a light selection member 151 and a receiver 152. The receiver 152 is located on the light path of the light selection member 151, and the light selection member 151 is used for selecting the imaging mode of the first imaging beam, the second imaging beam and the third imaging beam.

[0034] Specifically, the light selection member 151 can include at least two light selection sub-members (not labeled) connected in sequence. The light selection member 151 is movably arranged to move the at least two light selection sub-members alternatively between the objective lens 14 and the receiver 152 for selecting the imaging mode of the first imaging beam, the second imaging beam and the third imaging beam. That is, the light selection member 151 can move the required light selection sub-member between the objective lens 14 and the receiver 152 during the movement to select the mode of the imaging beam. The plurality of light selection sub-members can be arranged in a straight line, and the light selection member 151 can reciprocate in the straight line direction to switch the light selection sub-members. In other embodiments, the plurality of light selection sub-members can also be arranged in a circle, and the circular light selection member 151 rotates to switch the light selection sub-members.

[0035] In Figure 1In the illustrated embodiment, the light selection member 151 includes a light selection sub-member 1511, a light selection sub-member 1512, a light selection sub-member 1513, and a light selection sub-member 1514. When the optical detection device 10 performs a differential count of leukocytes, erythrocytes, and platelets in a blood sample, the light selection sub-member 1511 can be a green filter, the light selection sub-member 1512 can be a red filter, the light selection sub-member 1513 can be a long-pass filter, and the light selection sub-member 1514 can be a cavity. When red fluorescent cells need to be observed, the light selection member 151 is moved to switch to the red filter. When green fluorescent cells need to be observed, the light selection member 151 is moved to switch to the green filter. When red and green fluorescent cells need to be observed simultaneously, the light selection member 151 is moved to switch to the long-pass filter, for example, the long-pass filter can have a transmission wavelength range of 510-690 nm. The filters filter the first imaging light beam. The cavity has an aperture (not labeled) for allowing the second imaging light beam or the third imaging light beam to pass directly through. It can be understood that the number and type of filters in the light selection member 151 can be specifically set according to the requirements of the detection items, which are not specifically limited here.

[0036] In other embodiments, the light selection member 151 can only include a cavity and a long-pass filter to simplify the structure of the light selection member 151 and save costs.

[0037] The receiver 152 is located on the light exit path of the light selection member 151 and is used to receive the imaging light beam in the preset mode. The receiver 152 can be a camera module, for example, the receiver 152 can be a CMOS (Complementary Metal Oxide Semiconductor) camera.

[0038] Further, as Figure 1 illustrated, the optical detection device 10 can further include a light splitting assembly 16 located on the light exit path of the fluorescence excitation assembly 11. The light splitting assembly 16 is used to guide the excitation light beam to the objective lens 14, and the objective lens 14 is used to collimate the excitation light beam to exit to the sample to be detected, that is, the excitation light beam emitted from the objective lens 14 is collimated light, so that uniform excitation is achieved, thereby improving the accuracy of sample detection.

[0039] The light splitting assembly 16 is also used to guide the first imaging light beam, the second imaging light beam, and the third imaging light beam emitted by the objective lens 14 to the imaging receiving assembly 15. By using the light splitting assembly 16, the structure of the optical detection device 10 is more compact.

[0040] In Figure 1In the embodiment shown, the light splitting component 16 can reflect the excitation light beam into the objective lens 14, and can transmit the first, second and third imaging light beams to the imaging receiving component 15. It can be understood that in other embodiments, the light splitting component 16 can also reflect the first, second and third imaging light beams to the imaging receiving component 15, and transmit the excitation light beam into the objective lens 14. In this way, the structure of the optical detection device 10 can be more compact.

[0041] In Figure 1 In the embodiment shown, the light splitting component 16 is a dichroic mirror for filtering the first imaging light beam and a hole for transmitting the second or third imaging light beam.

[0042] Further, as Figure 1 As shown, the fluorescence excitation component 11 includes, in sequence, an excitation light source 111, a diaphragm 113, a second condenser lens 112 and a filter 114.

[0043] The excitation light source 111 is configured to emit an excitation light beam. Specifically, a light-emitting diode can be used as the excitation light source 111. For example, when performing white blood cell and platelet counting on a blood sample, a blue light-emitting diode can be used as the excitation light source 111, which outputs a wavelength of 470 nm ± 20 nm. The filter 114 can be a blue filter that allows blue light of 470 nm to pass through. In other embodiments, a green light-emitting diode can also be used as the excitation light source 111, etc.

[0044] The diaphragm 113 can be used to reduce the divergence angle of the light beam and block stray light. The excitation light beam emitted by the excitation light source 111 passes through the diaphragm 113 to filter out stray light with a large divergence angle, and then converges by the second condenser lens 112, and then filters by the filter 114 to allow excitation light beams of a specific wavelength to pass through. The excitation light beams of the specific wavelength are reflected by the light splitting component 16 to the back focal point of the objective lens 14, so that the excitation light beams emitted from the objective lens 14 are collimated light, thereby achieving uniform excitation of the sample to be detected.

[0045] Further, the diaphragm 113, the second condenser lens 112 and the filter 114 have the same geometric diameter, and the excitation light source 111, the diaphragm 113, the second condenser lens 112, the filter 114, the light splitting component 16 and the objective lens 14 can be coaxially arranged to improve the utilization rate of light.

[0046] In other embodiments, the fluorescence excitation assembly 11 can also only include the excitation light source 111 and a second focusing lens 112, which is arranged on the light path of the excitation light source 111 and used to converge the excitation light beam. The converged excitation light beam is guided to the back focal point of the objective lens 14 through the light splitting assembly 16, so as to simplify the structure of the fluorescence excitation assembly 11. The structure of the fluorescence excitation assembly 11 can be selected according to the actual detection item, which will not be listed here.

[0047] Further, as shown in Figure 1 , the bright / dark field illumination assembly 12 can include, in sequence, an illumination light source 121, a collimating lens 122 and a first focusing lens 123.

[0048] The illumination light source 121 is used to emit an illumination light beam. The illumination light source 121 can be a white light-emitting diode. In other embodiments, the illumination light source 121 can also be a yellow light-emitting diode, a red light-emitting diode or a green light-emitting diode (such as an output wavelength of 518±18 nm).

[0049] The collimating lens 122 is arranged on the light path of the illumination light source 121 and used to collimate the illumination light beam. Optionally, a light homogenizing element 125 can also be arranged on the light path of the collimating lens 122 and used to homogenize the collimated illumination light beam, so as to make the illumination light beam have a uniform incident light intensity.

[0050] The first focusing lens 123 is used to focus the collimated illumination light beam. Preferably, the first focusing lens 123 can be arranged to focus the collimated illumination light beam at the pupil of the objective lens 14. After the light rays are processed by the objective lens 14, the image plane imaging brightness is uniform, so as to improve the imaging effect and improve the accuracy of the sample analyzer detection.

[0051] In this embodiment, the illumination light source 121, the collimating lens 122, the light homogenizing element 125, the first focusing lens 123 and the objective lens 14 can be coaxially arranged, so as to improve the utilization rate of light. It can be understood that the bright / dark field illumination assembly 12 can also not be provided with the above-mentioned light homogenizing element 125, so as to simplify the structure of the bright / dark field illumination assembly 12.

[0052] The structure of the above-mentioned bright / dark field illumination assembly 12 can be used to detect the sample to be detected under the bright field, such as detecting the red blood cells in the blood sample. The bright field detection system has a simple structure, low cost and clear imaging, and can improve the accuracy of sample detection.

[0053] In another embodiment, as shown in Figure 2 , the bright / dark field illumination assembly 12 can include, in sequence, an illumination light source 121, a collimating lens 122 and a first focusing lens 123. Figure 2is a structural schematic diagram of another embodiment of the optical detection device provided in the present application. Different from the above-mentioned embodiment of bright field illumination, in the present embodiment, a light shielding piece 124 is arranged on the light path of the collimating lens 122, and the light shielding piece 124 is used to shield the illumination light beam in a preset range, so that the scattered light of the illumination light source 121 cannot enter the field of view of the objective lens 14, thereby providing a dark field environment for the detection of the sample to be detected.

[0054] In the present embodiment, the illumination light beam emitted by the illumination light source 121 is collimated by the collimating lens 122, and then can pass through the light homogenizing piece 125 to homogenize the light intensity of the illumination light beam, and then pass through the light shielding piece 124, and then pass through the first condenser lens 123, so that the illumination light beam is focused on the sample to be detected, that is, the glass slide on the carrying table 13 is located on the focal plane of the first condenser lens 123, and the position of the focal plane is the object plane of the objective lens 14, thereby providing a dark field environment for the imaging of the sample to be detected.

[0055] In the present embodiment, the scattered light of the illumination light source 121 cannot enter the field of view of the objective lens 14, thereby forming a dark field detection system. In the present dark field detection system, the scattered light of the illuminated sample to be detected is magnified and imaged by the objective lens 14, and is guided to the imaging receiving assembly 15 by the light splitting assembly 16. For example, the present dark field detection system can be used for classifying and counting red blood cells and platelets in a blood sample.

[0056] In the present embodiment, the light homogenizing piece 125 and the light shielding piece 124 have the same geometric size, and the collimating lens 122 and the first condenser lens 123 have the same geometric size. The illumination light source 121, the collimating lens 122, the light homogenizing piece 125, the light shielding piece 124, the first condenser lens 123 and the objective lens 14 can be coaxially arranged to improve the utilization rate of light.

[0057] Further, in some embodiments, the bright / dark field illumination assembly 12 is movably arranged above the carrying table 13 in the vertical direction, so that the bright / dark field illumination assembly 12 has a first state and a second state. Specifically, the bright / dark field illumination assembly 12 can be moved in the direction of approaching and moving away from the carrying table 13, so as to switch the bright / dark field illumination assembly 12 between the first state and the second state.

[0058] In the first state, the bright / dark field illumination assembly 12 is not provided with the light shielding piece 124, and the bright / dark field illumination assembly 12 is close to the carrying table 13, so that the first condenser lens 123 can focus the collimated illumination light beam at the pupil of the objective lens 14, and the objective lens 14 can perform microscopic imaging on the sample to be detected which is uniformly illuminated by the sample to be detected, thereby forming a second imaging light beam for bright field detection.

[0059] In the second state, the bright / dark field illumination assembly 12 is provided with a light shield 124, specifically, the light shield 124 is arranged between the first condenser lens 123 and the collimating lens 122, and the light shield 124 is used to shield a preset range of illumination light beams. The bright / dark field illumination assembly 12 is away from the bearing table 13, and the illumination light beams emitted by the bright / dark field illumination assembly 12 are shielded by the light shield 124, focused on the sample to be detected, and the light beams passing through the sample to be detected cannot enter the objective lens 14. The illuminated sample to be detected is imaged by the objective lens 14 to form a third imaging light beam for dark field detection.

[0060] By movably arranging the bright / dark field illumination assembly 12, for example, the bright / dark field illumination assembly 12 is vertically adjustable up and down, the switching of bright / dark field can be realized, the flexibility of the optical detection device 10 is improved, and the applicability of the optical detection device 10 is further improved.

[0061] The optical detection device 10 of the present application has the advantages of simple and compact structure, low cost, clear optical imaging effect, and improved sample detection accuracy. Moreover, the optical detection device 10 can not only perform fluorescence detection on the sample to be detected, but also perform bright / dark field detection on the sample to be detected, and the optical detection device 10 has a wide range of applications.

[0062] The present application also provides a control method of an optical detection device, which is based on the optical detection device 10 of any one of the above embodiments. As shown in the Figure 3 control method includes:

[0063] S11: placing the sample to be detected on the bearing table.

[0064] S12: emitting an excitation light beam by the fluorescence excitation assembly and emitting an illumination light beam by the bright / dark field illumination assembly, wherein the light emission paths of the excitation light beam and the illumination light beam pass through the sample to be detected.

[0065] S13: performing microscopic imaging on the fluorescent particles in the sample to be detected by the objective lens to form a first imaging light beam, imaging the sample to be detected in the bright field by the objective lens to form a second imaging light beam, and imaging the sample to be detected in the dark field by the objective lens to form a third imaging light beam.

[0066] S14: receiving the first imaging light beam, the second imaging light beam and the third imaging light beam by the imaging receiving assembly.

[0067] The optical detection device of this application, when detecting a sample, first places the sample on a stage. A fluorescence detection system is formed by a fluorescence excitation component, an objective lens, and an imaging receiving component. The excitation beam emitted by the fluorescence excitation component is projected onto the sample, causing it to fluoresce. The fluorescent particles are imaged by the objective lens to form a first imaging beam. The imaging receiving component receives this first imaging beam to obtain an image of the sample. Fluorescence detection analysis is then performed on the sample based on this image. Alternatively, a bright / dark field detection system is formed by a bright / dark field illumination component, an objective lens, and an imaging receiving component. The illumination beam emitted by the bright / dark field illumination component is projected onto the sample. In bright field conditions, the objective lens images the sample to form a second imaging beam; in dark field conditions, the objective lens images the sample to form a third imaging beam, thus obtaining an image of the sample. Bright / dark field detection analysis is then performed on the sample based on this image.

[0068] For specific detection methods of the fluorescence detection system and the bright / dark field detection system, please refer to the description in the above embodiments, which will not be repeated here.

[0069] The optical inspection device of this application can perform both fluorescence detection and bright / dark field detection on the sample to be tested, enabling the optical inspection device to perform multiple tests and improving the applicability of the optical inspection device.

[0070] This application also provides a sample analyzer, such as... Figure 4 As shown, Figure 4 This is a schematic diagram of an embodiment of the sample analyzer provided in this application. Specifically, the sample analyzer 30 includes a sample preparation device 31 and an optical detection device 32. The sample preparation device 31 is used to prepare a sample to obtain a sample to be tested, and the optical detection device 32 is used to perform optical detection on the sample to be tested.

[0071] The optical detection device 32 in this embodiment can be any of the optical detection devices 10 described above. For the structure of the optical detection device 32, please refer to the description of any of the above embodiments, which will not be repeated here.

[0072] The sample analyzer 30 in this application can be a blood analyzer or a body fluid analyzer, etc. The sample to be tested by the sample analyzer 30 can be blood, such as animal blood or human blood, or it can be body fluids such as saliva, sweat, or urine. The sample analyzer 30 of this application can perform multiple tests on the sample, has a wide range of applications, and is simple in structure and low in cost.

[0073] The above merely describes the embodiments of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation, or direct or indirect application in other related technical fields, which is made according to the content of the present application specification and drawings, is also included in the patent protection scope of the present application.

Claims

1. An optical detection device, characterized in that, The optical detection device includes: A fluorescence excitation component and a bright / dark field illumination component are arranged at intervals, wherein the fluorescence excitation component is used to emit an excitation beam and the bright / dark field illumination component is used to emit an illumination beam; A support stage is located on the light exit path of the excitation beam and the illumination beam, and the support stage is used to hold the sample to be tested; The objective lens, located below the stage, is used to perform microscopic imaging of fluorescent particles in the sample to be tested to form a first imaging beam, and to image the sample to be tested under bright field to form a second imaging beam, and to image the sample to be tested under dark field to form a third imaging beam. An imaging receiving component, located in one of the light exit paths of the objective lens, is used to receive the first imaging beam, the second imaging beam, and the third imaging beam. The fluorescence excitation component and the bright / dark field illumination component share the objective lens and the imaging receiving component. The imaging receiving component includes a light selector and a receiver. The receiver is located in the light output path of the light selector. The light selector includes at least two light selector sub-components connected in sequence. The at least two light selector sub-components are movably configured so that one of the at least two light selector sub-components moves between the objective lens and the receiver to select the imaging mode of the first imaging beam, the second imaging beam, or the third imaging beam. The receiver is used to receive the imaging beam of the preset mode.

2. The optical detection device according to claim 1, characterized in that, The at least two optical selection components include a cavity and at least one filter connected in sequence. The cavity has a hole for allowing the first imaging beam and / or the second imaging beam to pass through directly, and the filter is used to filter the first imaging beam.

3. The optical detection device according to claim 1, characterized in that, The bright / dark field lighting assembly includes: A light source used to emit a beam of light; A collimating lens is located in the light-emitting path of the illumination source and is used to collimate the illumination beam. A light-shielding component is disposed in the light-emitting path of the collimating lens to block the illumination beam within a preset range; The first focusing lens is located in the light-emitting path of the light-shielding member and is used to focus the illumination beam.

4. The optical detection device according to claim 1, characterized in that, The bright / dark field lighting assembly includes: A light source used to emit a beam of light; A collimating lens is located in the light-emitting path of the illumination source and is used to collimate the illumination beam. The first condenser lens is located in the light output path of the collimating lens and is used to focus the collimated illumination beam.

5. The optical detection device according to claim 4, characterized in that, The bright / dark field lighting component is movably mounted above the support platform in a vertical direction, so that the bright / dark field lighting component has a first state and a second state. In the first state, the first condenser lens is used to focus the collimated illumination beam onto the pupil of the objective lens; In the second state, a light-blocking component is provided between the first condensing lens and the collimating lens. The light-blocking component is used to block the illumination beam within a preset range, and the first condensing lens is used to focus the illumination beam passing through the light-blocking component onto the sample to be tested.

6. The optical detection device according to claim 4 or 5, characterized in that, The bright / dark field lighting assembly also includes: A light-diffusing element is disposed in the light-emitting path of the collimating lens and is used to dilute the light of the collimated illumination beam.

7. The optical detection device according to claim 1, characterized in that, The optical detection device further includes: A beam splitter is located in the light output path of the fluorescence excitation component. The beam splitter is used to output the excitation beam to the objective lens. The objective lens is used to collimate the excitation beam and output it to the sample to be tested. The beam splitter is also used to guide the first imaging beam, the second imaging beam and the third imaging beam output from the objective lens to the imaging receiving component.

8. A control method for an optical detection device, characterized in that, Based on the optical detection device according to any one of claims 1-7, the control method includes: Place the sample to be tested on the support platform; An excitation beam is emitted through a fluorescence excitation component, and an illumination beam is emitted through a bright / dark field illumination component, wherein the light exit paths of the excitation beam and the illumination beam pass through the sample under test; The fluorescent particles in the sample under test are imaged by an objective lens to form a first imaging beam. The sample under test under bright field conditions is imaged by the objective lens to form a second imaging beam. The sample under test under dark field conditions is imaged by the objective lens to form a third imaging beam. The imaging receiving component receives the first imaging beam, the second imaging beam, and the third imaging beam.

9. A sample analyzer, characterized in that, The sample analyzer includes: A sample preparation device is used to prepare samples to obtain samples to be tested; The optical detection device according to any one of claims 1-7 is used to perform optical detection on the sample to be detected.

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