Analysis device

Abstract

The invention relates to an analysis device. Due to the relationship between a flow path wall and an electrode with respect to a porous base material, both adherence between the electrode and the base material and suppression of electrode cracks caused by deformation of the base material in a high-humidity environment and the like are difficult to achieve, and in some cases, the inability to achieve both may hinder the correct proceeding of ion concentration measurement. Provided is a structure in which, in an electrode portion that makes contact with an external measurement instrument, an electrode has at least a portion formed inside a porous substrate, and a flow path wall is always present on a straight line in which the electrode is located.

Description

Technical Field

[0001] The present invention relates to an analysis device in which an electrode is formed by a flow path using a porous substrate. Background Art

[0002] In recent years, the development of microanalysis chips that can effectively (micro, fast and easily) perform analysis in biochemistry in one chip by utilizing micro-sized micro-flow paths has attracted attention in a wide range of fields. The wide range of fields includes not only biochemical research, but also medicine, drug discovery, health care, environment and food. Among them, paper-based paper microanalysis chips have advantages over prior art devices in terms of light weight, low cost, no power supply and high disposability. Therefore, paper microanalysis chips are expected to be used as test devices, such as for: medical activities in developing countries, remote areas and disaster sites with imperfect medical equipment; and airports that need to prevent the spread of infectious diseases at the border. In addition, due to its low price and easy operation, paper microanalysis chips have always attracted attention as medical care devices that enable individuals to manage and monitor their own health conditions.

[0003] As an example of a paper microanalysis chip, a structure is described in U.S. Patent Application Publication No. 2016 / 033438, in which electrodes are formed on a porous substrate to span a hydrophobic flow path wall made of wax. In this structure, one end of the electrode reacts with a sample in the flow path to form a predetermined potential, and a measuring instrument is connected to the other electrode end for measurement. The structure consists of two electrodes, namely, a reference electrode indicating a fixed potential of a reference solution and an ion selective electrode indicating a potential corresponding to the ion concentration of the sample, and the potential difference between the two electrodes is measured, thereby being able to measure the ion concentration of the sample.

[0004] In addition, in Analytical Chemistry 89, pages 10,608-10,616, 2017, an analytical chip capable of measuring the ion concentration of a sample by measuring the potential difference between two electrodes is described, but in the construction of this document, the electrode serving as the contact part of the measuring instrument is formed on a hydrophobic flow path wall made of wax.

[0005] However, in the method described in U.S. Patent Application Publication No. 2016 / 033438, for example, when the analysis chip is placed in a high humidity environment, there is a concern that the paper may be deformed due to moisture absorption and cracks may occur in the electrode. When cracks occur in the electrode, the measured value of the potential is naturally affected, and thus it becomes difficult to correctly measure the ion concentration.

[0006] In addition, in the method described in Analytical Chemistry 89, pp. 10,608-10,616, 2017, the electrode used as the contact portion of the measuring instrument is formed on the flow path wall, and therefore the adhesion may be deteriorated compared to the case where it is formed on a porous material. When the adhesion of the electrode is poor, there is a concern that the electrode may be peeled off due to pressing or rubbing performed to achieve measurement contact. And, when the electrode is peeled off, the measured value of the potential is naturally affected, and it becomes difficult to correctly measure the ion concentration. Summary of the invention

[0007] Provided is an analysis device comprising: a porous substrate, a flow path wall formed by filling pores of the porous substrate with a hydrophobic material, and an electrode, wherein when the analysis device is observed from above, the analysis device comprises a first region and a second region separated from each other by the flow path wall, the electrode is formed to span the first region, the flow path wall, and the second region, a straight line passing through the electrode always intersects the flow path wall, and, in the second region, the electrode also has at least a portion formed inside the porous substrate.

[0008] Further features of the present invention will become apparent from the following description of exemplary embodiments with reference to the attached drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 It is a top view of the analysis device (M1) after the flow path wall (11) according to the first embodiment is formed (before the electrode is formed).

[0010] Figure 2A It is a top view of the working electrode side of the analysis device (M1) according to the first embodiment.

[0011] Figure 2B It is a cross-sectional view taken along the dotted line portion (D1) of the analysis device (M1) according to the first embodiment.

[0012] Figure 3A It is a top view of the analysis device (M1) after various electrodes are formed according to the first embodiment.

[0013] Figure 3B is a cross-sectional view taken along a dotted line ( D2 ) of the analysis device ( M1 ) according to the first embodiment.

[0014] Figure 4A This is a top view of the analysis device (M2) after various electrodes are formed according to Comparative Example 1.

[0015] Figure 4B This is a perspective view of an analysis device (M2) after various electrodes are formed according to Comparative Example 1.

[0016] Figure 51 is a diagram showing the results of potential measurement of a chip in which cracks occurred and a chip in which cracks did not occur.

[0017] Fig. 6A It is a top view of the analysis device (M3) according to Comparative Example 2.

[0018] Figure 6B It is a cross-sectional view of the analysis device (M3) according to Comparative Example 2 taken along the dotted line (D6). DETAILED DESCRIPTION

[0019] The following describes an exemplary embodiment of the present invention with reference to the accompanying drawings. The following embodiments are exemplary, and the present invention is not limited to the contents of the embodiments. In addition, in the following drawings, constituent elements that are not necessary for describing the embodiments are omitted from the drawings.

[0020] According to an embodiment of the present invention, there is provided an analysis device comprising: a porous substrate, a flow path wall formed by filling pores of the porous substrate with a hydrophobic material, and an electrode, wherein when the analysis device is observed from above, the analysis device comprises a first region and a second region separated from each other by the flow path wall, the electrode is formed to span the first region, the flow path wall, and the second region, a straight line passing through the electrode always intersects with the flow path wall, and, in the second region, the electrode also has at least a portion formed inside the porous substrate.

[0021] The first area may be an area penetrated by the sample. The second area may be a connection area with an external measuring instrument, and the analysis device may be connected to the external measuring instrument through the portion of the electrode present in the second area. The analysis device may include a first electrode and a second electrode as electrodes, the first electrode may be a reference electrode, and the second electrode may be a working electrode.

[0022] [First embodiment]

[0023] <Substrate / Flow path>

[0024] Reference Figure 1 The substrate of the analytical device M1 is described. Figure 1 is a simplified top view of the analysis device M1 before the various electrodes are formed.

[0025] In this embodiment, filter paper is used as the porous substrate S1. The material of the filter paper used is cellulose, and the thickness is 100 μm and the porosity is 50%. Such filter paper has satisfactory hydrophilicity due to the capillary phenomenon caused by the fine gaps between the cellulose fibers, and plays the role of a flow path that is smoothly penetrated by the liquid. It should be understood that the porous substrate S1 is not limited to filter paper, as long as the porous substrate S1 plays the role of a flow path, and its examples may include not only paper such as ordinary paper, high-quality paper, watercolor paper, Kent paper and synthetic paper, but also synthetic resin porous membranes, fabrics and fiber products. The porosity can be appropriately selected according to the purpose, and the porosity is preferably 20% or more and 90% or less. When the porosity exceeds 90%, the strength of the substrate may not be maintained, and when the porosity is less than 20%, the permeability of the sample liquid may deteriorate.

[0026] The porosity (%) is calculated by "porosity (%) = ((true density) - (apparent density)) / (true density) x 100".

[0027] The hydrophobic resin as a hydrophobic material is infiltrated into a partial area of ​​the porous substrate S1, thereby forming a flow path wall 11 with a longitudinal width H1 of 14 mm and a lateral width L1 of 22 mm. The flow path wall 11 is formed by the method described in Japanese Patent Application Laid-Open No. 2021-37612, in which an electrophotographic printing using a hydrophobic resin as a toner is performed, and the resin is melted and infiltrated by heating. As a hydrophobic material for forming a flow path wall, a hydrophobic resin is preferably used. There is no particular restriction on the hydrophobic resin, and for example, polyester resins, vinyl resins, acrylic resins, styrene acrylic resins, polyethylene, polypropylene, polyolefins, ethylene-vinyl acetate copolymer resins and ethylene-acrylic acid copolymer resins are used. An example of a method for configuring a hydrophobic resin may include not only a method involving the use of an electrophotographic device, but also a method involving the use of an inkjet printer, a method for infiltrating wax by a wax printer, and a method for infiltrating resin by screen printing. In addition, a variety of methods can be used in combination to form a hydrophobic resin.

[0028] In the area inside the flow path wall 11, there is an area that is not penetrated by the hydrophobic resin forming the flow path wall, that is, an area without a flow path wall. The analysis device according to this embodiment includes at least a first area 101 and a second area 102 as an area without a flow path wall. The first area 101 is an area that is penetrated by a sample due to a capillary phenomenon caused by the porosity of the porous substrate S1. In this embodiment, the first area 101 is formed by: a working electrode configuration part S1b configured with a working electrode, a reference electrode configuration part S1c configured with a reference electrode, a dispensing part S1d located between the working electrode configuration part S1b and the reference electrode configuration part S1c, and a flow path S1f each having a width of 2 mm, and the dispensing part S1d is connected to the working electrode configuration part S1b and the reference electrode configuration part S1c through the flow path S1f. However, the first area 101 does not always need to include all of these, and may include other parts and flow paths. In this embodiment, the working electrode configuration part S1b and the reference electrode configuration part S1c are each a square with a side length of 6mm, and the dispensing part S1d is a circle with a diameter of 3mm. The size and shape of each configuration part and the flow path are not limited to this. Next, the second region 102 is a region without a flow path wall, and is a region as a position for contact with an external measuring instrument during measurement, and the second region 102 is not always penetrated by the sample. In this embodiment, the second region 102 includes a working electrode contact part S1a and a reference electrode contact part S1e, and each contact part is a square with a side length of 4mm. In this embodiment, an example is described in which the working electrode contact part S1a and the reference electrode contact part S1e are separated from each other by the flow path wall and are not connected to each other, but the working electrode contact part S1a and the reference electrode contact part S1e can be connected to each other. At the same time, the first region 101 and the second region 102 are not connected to each other, and are formed to be separated from each other by the flow path wall 11. The details of the contact are described later together with the description of the electrode.

[0029] Now, refer to Figure 2A and Figure 2B The formation of the working electrode of the analytical device M1 is described.

[0030] Figure 2A is a top view of the analysis device M1 after the working electrode 31 is formed, and the cross section thereof taken along the dotted line portion D1 is as shown in FIG. Figure 2B shown.

[0031] <Working Electrode>

[0032] The working electrode 31 is an electrode having a shape in which a square with a side length of 4 mm accommodated in the working electrode arrangement portion S1b and a rectangle with a width of 1 mm and a length of 6 mm extending from the working electrode arrangement portion S1b to the working electrode contact portion S1a are continuous. As the material of the working electrode 31, Ag was used as the detection Cl- The working electrode 31 may be an electrode of an ion concentration of 100 Å, but the material is not limited thereto, and any material or structure that functions as a working electrode is sufficient. In addition to the electrode, the working electrode 31 may also form an ion selective membrane.

[0033] The working electrode 31 is formed by using a screen printer DP-320 manufactured by NEWLONG SEIMITSU KOGYO Co., LTD. Printing is performed using a #200 plate, and drying is performed at 80°C for 10 minutes. However, the method for forming the working electrode 31 is not limited thereto. As an electrode, in addition to Ag / AgCl and PEDOT / PSS, an electrode using carbon, etc. is also used without any particular restrictions. For a method of producing an electrode by using Ag / AgCl or carbon, reference may be made to, for example, U.S. Patent Application Publication No. 2016 / 033438. For a method of producing an electrode by using PEDOT (poly (3,4-ethylenedioxythiophene)) / PSS (poly (4-styrenesulfonate)), reference may be made to, for example, Analytical Chemistry 89, pp. 10,608-10,616, 2017.

[0034] In the case where the working electrode 31 has an ion selective membrane, the ion selective membrane can be any commonly used membrane, and can be any selective membrane that is sensitive to the target ion and has sufficient selectivity to the interfering ions. The ion selective membrane contains a compound with ion selectivity. There is no particular restriction on the compound with ion selectivity, and its preferred examples can include ion carriers and anion scavengers that exhibit ion selectivity. Examples of ion carriers include 12-crown 4-ether with a crown ether structure, and examples of anion scavengers include sodium tetraphenylborate (NaTPB). Any known material for ion selective membranes can be used, and as such materials, reference can be made to pages 138 to 140 of "How to Measure Ion Concentration with Electrodes" by, for example, DOJINDO LABORATORIES.

[0035] The ion selective membrane may further include a polymer compound to maintain its structure. Examples of the polymer compound include polyvinyl chloride (abbreviation: PVC) and copolymers of vinyl chloride and vinyl acetate. In order to be able to handle ion carriers, the ion selective membrane may further include a plasticizer. Well-known plasticizers are applicable, and more preferably a lipophilic ion exchange material as a material used as a lipophilic ion exchanger in the ion selective membrane. Examples of well-known materials include 2-nitrophenyl octyl ether (abbreviation: NPOE), bis(2-ethylhexyl) sebacate (abbreviation: DOS), bis(2-ethylhexyl) adipate (abbreviation: DOA) and di-n-octylphenyl phosphonate (abbreviation: DOPP).

[0036] like Figure 2B As shown, a part of the working electrode 31 formed in the above manner is formed inside the porous substrate of the first region 101 and the second region 102. That is, in the working electrode configuration part S1b as the first region 101 and the working electrode contact part S1a as the second region 102, a part of the electrode penetrates into the pores of the porous substrate S1, and the electrode is also formed inside the porous substrate S1. Therefore, not only is the contact with the sample properly made in the working electrode configuration part S1b as the first region 101, but also in the working electrode contact part S1a as the second region 102, the adhesion between the electrode and the porous substrate S1 can be enhanced due to the anchoring effect. Therefore, the working electrode contact part S1a as the second region 102 is set as the contact part with the external measuring instrument, so that it is possible to form an electrode that is unlikely to peel off even when the electrode is pressed and rubbed due to the measurement contact. In order to obtain this effect, it is necessary to expose the porous substrate at least a part of the surface of the second region 102.

[0037] In this embodiment, both the working electrode contact portion S1a and the reference electrode contact portion S1e forming the second region 102 have a structure in which the interior of the porous substrate S1 does not contain any substance. However, for the purpose of adjusting the physical properties of the substrate, etc., the required materials can be arranged in the porous substrate of these contact portions, as long as at least a portion of the porous substrate is exposed on the surface. For example, in order to enhance the rigidity of the working electrode contact portion S1a, a predetermined resin can be impregnated in the porous material, thereby improving the ease of contact with an external measuring instrument. At this time, within the range where the pores of the porous substrate S1 are exposed on the surface, the type, shape and amount of the material formed in the porous substrate S1 can be freely selected.

[0038] Now, refer to Figure 3A and Figure 3BThe formation of the reference electrode of the analysis device M1 is described.

[0039] Figure 3A is a top view of the analysis device M1 after the working electrode 31 is formed, and its cross-sectional view taken along the dotted line portion D2 is as shown in FIG. Figure 3B shown.

[0040] <Reference Electrode>

[0041] The reference electrode 32 is formed to span from the reference electrode arrangement portion S1c as the first region 101 to the reference electrode contact portion S1e as the second region 102, and has a rectangular shape of (1 mm)×(10 mm). The reference electrode 32 reacts with the sample permeating the flow path in the reference electrode arrangement portion S1c as the first region 101 in the same manner as in the case of the working electrode 31, and can achieve contact with an external measuring instrument at the reference electrode contact portion S1e as the second region 102. In the case of a width as small as 1 mm, when applying the electrolyte layer 41 described later, it is easy to arrange the electrolyte layer 41 on the porous substrate in the reference electrode arrangement portion S1c including the back side of the reference electrode 32.

[0042] As the material of the reference electrode 32, Ag / AgCl is used. By using Ag / AgCl, an equilibrium reaction according to formula (1) occurs in the aqueous solution, and thus Cl around the reference electrode 32 - When the concentration is stable, a stable potential can be obtained.

[0043]

[0044] The reference electrode 32 is applied to the reference electrode arrangement portion S1c by using a screen printer DP-320 manufactured by NEWLONG SEIMITSU KOGYO Co., LTD. Printing is performed using a #200 plate, and drying is performed at 80°C for 10 minutes. However, the method of producing the electrode is not limited thereto, and any kind of method described in the working electrode section may be used.

[0045] In the reference electrode contact portion S1e as the second region, the reference electrode 32 has a portion formed inside the porous substrate S1 in the same manner as in the case of the working electrode contact portion S1. Therefore, the reference electrode 32 is less likely to peel off.

[0046] <Electrolyte Layer>

[0047] When the sample introduced into the dispensing section S1d permeates the reference electrode arrangement section S1c, the electrolyte layer 41 is dissolved by the moisture of the sample, and Cl - Set to saturation concentration. -When the concentration becomes saturated, the potential of the reference electrode 32 is stabilized. Therefore, in this embodiment, KCl is used as the material of the electrolyte layer 41. KCl is easily soluble in water, and the diffusion speeds of K ions and Cl ions are substantially equal, resulting in a low possibility of liquid junction potential, and KCl is selected based on this. It should be understood that the material of the electrolyte layer 41 is not limited thereto, and any material such as NaCl can be used as long as the potential of the reference electrode 32 can be stabilized.

[0048] The electrolyte layer 41 is coated by using a BioSpot manufactured by MICROJET Co., Ltd. A solution is prepared by dissolving KCl in pure water to a concentration of 16% by mass. Printing is performed over the entire area of ​​the reference electrode configuration portion S1c with a droplet size of 6nL / drop, a frequency of 10Hz, and a pitch of 300μm in both the vertical and horizontal directions, and three overlay coatings are performed to adjust the amount of KCl. The amount of KCl is set to a saturated amount relative to the sample amount that permeates the reference electrode configuration portion S1c, so that the potential of the reference electrode 32 can be stabilized. Therefore, the coating amount is not limited to this, as long as the amount of KCl contained is sufficient to become saturated KCl. The KCl aqueous solution has a lower viscosity than the solution of the ion selective membrane 21, and as the droplet size increases and the frequency increases, the KCl aqueous solution attached to the porous substrate S1 diffuses in the porous substrate S1 before volatilizing, so that it can be as Figure 3B As shown, KCl can also be arranged on the back side of the reference electrode 32. Therefore, when the sample penetrates, the Cl around the reference electrode 32 - The concentration becomes stable, thereby stabilizing the potential of the reference electrode 32. That is, the reference electrode 32 functions as a reference electrode with higher stability.

[0049] In the analysis device M1, the straight line passing through the electrode always passes through the flow path wall. Figure 3A In the dotted line portion D3, the flow path wall 11 is formed at a position other than the working electrode contact portion S1a and the reference electrode contact portion S1e. In the same manner, the dotted line portion D4 taken at a changed angle is configured to pass through the flow path wall and through the electrode. With this configuration, the porous substrate S1 can be prevented from being deformed even when placed in a high humidity environment, and cracks in the electrode can be suppressed.

[0050] [Comparative Example 1]

[0051] Reference Figure 4A and Figure 4B , an analyzing device M2 according to Comparative Example 1 is described with respect to the analyzing device M1.

[0052] Figure 4Ais a simplified top view of the analysis device M2, and Figure 4B 2 is a simplified perspective view of the analyzing device M2. The configuration of the analyzing device M2 is the same as that of the analyzing device M1 except for the shape of the flow path wall, so the same components are denoted by the same reference numerals and the description of the same parts is omitted.

[0053] like Figure 4A As shown, when viewed toward the drawing, the flow path wall 51 of the analysis device M2 includes a hydrophobic flow path wall only above the paper surface within the range where the longitudinal width H2 of the analysis device M2 is 7 mm. At the same time, within the range indicated by H3 corresponding to the second area of ​​the analysis device M1, etc., that is, within the range below the paper surface corresponding to the analysis device M2 when viewed toward the drawing, no flow path wall is provided. Therefore, at the position of the dotted line portion D5, there is an electrode, but there is no flow path wall. That is, when the analysis device is viewed from above, the analysis device M2 has a straight line that passes through the electrode but not through the flow path wall, and does not satisfy the condition that the straight line passing through the electrode always intersects with the flow path wall.

[0054] A case where the analyzing device M2 is placed in a high humidity environment will now be described.

[0055] In a high humidity environment, water is adsorbed on the porous substrate S1 made of cellulose having high hydrophilicity, and thus fibers of paper swell, thereby causing corrugations in the porous substrate S1 and deformation thereof. Figure 4B The state is shown in which the working electrode 31 and the reference electrode 32 formed thereon are also deformed due to the influence of the deformation of the porous substrate S1. When such deformation occurs, the electrodes cannot withstand the stress of the deformation, causing cracks therein, and when potential measurement is performed by an external measuring instrument, an increase in load resistance and poor conduction due to the cracks may prevent correct ion concentration measurement.

[0056] At this time, at the position where the flow path wall 51 exists, water adsorption does not occur due to the hydrophobicity of the flow path wall 51, and deformation due to expansion of the porous substrate S1, etc. does not occur. In addition, the presence of the flow path wall 51 increases rigidity compared to the case where only the porous substrate S1 exists, thereby suppressing deformation of the flow path surrounded by the flow path wall 51. That is, on a straight line not involving the flow path wall (for example, in the dotted line portion D5), expansion due to water adsorption, etc. may cause deformation of the porous substrate S1, and when an electrode is present at the deformed position, measurement may not be performed correctly due to cracks in the electrode.

[0057] Figure 5The potential difference between the working electrode and the reference electrode displayed when a sample prepared by dissolving 100 mM NaCl is actually dispensed into the dispensing portion S1d of the analysis device M2 is shown. For the potential measurement, the potential difference between the working electrode contact portion S1a and the reference electrode contact portion S1e is measured by using VSP-300 manufactured by Bio-Logic. The analysis device used for the measurement is a total of three chips (analysis device), namely, two chips in which no cracks occur in the electrodes (chip 1 without cracks and chip 2 without cracks) and one chip in which cracks occur (chip 1 with cracks). As Figure 5 As shown, it should be understood that the chip without cracks shows almost the same potential after, for example, 10 seconds, but the chip with cracks is greatly different in potential, and even when trying to measure the ion concentration, the ion concentration of the sample cannot be measured correctly.

[0058] [Comparative Example 2]

[0059] Next, refer to Fig. 6A and Figure 6B , an analyzing device M3 according to Comparative Example 2 is described with respect to the analyzing device M1.

[0060] Fig. 6A is a simplified top view of the analysis device M3, and a cross section thereof taken along the dashed line portion D6 is shown in FIG. Figure 6B The configuration of the analyzing device M3 is the same as that of the analyzing device M1 except for the shape of the flow path wall, so the same components are denoted by the same reference numerals and the description of the same parts is omitted.

[0061] like Fig. 6A As shown, the analysis device M3 includes a flow path wall 61 having a longitudinal width H1 of 14 mm in the same manner as the analysis device M1, but also has a flow path wall 61 formed in the working electrode contact portion S1a and the reference electrode contact portion S1e. That is, the analysis device M3 does not include the second region. With this configuration, unlike the above-described analysis device M2, the analysis device M3 is less likely to have a deformed porous substrate S1 even when placed in a high humidity environment, and cracks do not occur even in the electrodes.

[0062] At the same time, if Figure 6BAs shown, the analysis device M3 does not include the second region, and naturally, the working electrode does not include the portion inside the porous substrate formed in the second region. In the working electrode contact portion S1a and the reference electrode contact portion S1e, the working electrode 31 and the reference electrode 32 are formed on the upper layer of the flow path wall 61. That is, the pores of the porous substrate S1 are filled with the flow path wall 61, and electrodes are formed thereon. Therefore, the working electrode 31 and the reference electrode 32 cannot obtain the above-mentioned anchoring effect on the substrate, resulting in low adhesion. This results in the working electrode 31 and the reference electrode 32 being peeled off from the porous substrate S1 when pressed or rubbed by the measurement contact with the external measuring instrument during potential measurement, and therefore, the potential measurement may not be stably performed in some cases.

[0063] The results of the above-described embodiments and comparative examples are tabulated below.

[0064] Table 1

[0065]

[0066] This embodiment has been described by taking the case where the analysis device is placed in a high humidity environment as an example of deformation of the substrate. However, the case is not limited to this, and the same phenomenon occurs during the process of producing the analysis device, for example, in the case of cleaning after electrode formation and in the case of a solvent or the like penetrating the porous substrate S1 in a pretreatment step. Therefore, even in such a case, stable potential measurement can be performed using the configuration of this embodiment.

[0067] As described above, using a structure in which an electrode is provided that is formed to span a second area exposed on the surface of a porous material and a first area separated from the second area by a flow path wall, and the flow path wall always exists on a straight line where the electrode is located, the adhesion between the electrode and the substrate can be enhanced while suppressing electrode cracks caused by deformation of the substrate.

[0068] That is, using this configuration, it is possible to provide an analysis device capable of stably performing potential measurement.

[0069] That is, according to one embodiment of the present disclosure, a microanalysis chip can be provided which has high adhesion between an electrode and a porous substrate while suppressing electrode cracks due to deformation of the substrate and enables stable potential measurement.

[0070] While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. The scope of the following claims is to be accorded the broadest interpretation so as to encompass all such modifications and equivalent structures and functions.

Claims

1. An analysis device, comprising: Porous substrates; a flow path wall formed by filling the pores of the porous substrate with a hydrophobic material; and electrode, When the analysis device is viewed from above, The analysis device includes a first region and a second region separated from each other by the flow path wall, The electrode is formed to span the first region, the flow path wall, and the second region, A straight line through the electrode always intersects the flow path wall, and in, In the second region, the electrode also has at least a portion formed inside the porous substrate. 2 . The analytical device according to claim 1 , wherein the first area comprises an area permeated by the sample.

3. The analysis device according to claim 1, wherein the second region comprises a connection region, and The analysis device is connected to an external measuring instrument via the portion of the electrode present in the second region.

4. The analysis device according to claim 1, wherein the electrodes include a first electrode and a second electrode, wherein the first electrode comprises a reference electrode, and The second electrode comprises a working electrode.

Images