Method for secondary fruiting of phlebopus portentosus

Abstract

The invention relates to the technical field of edible mushrooms, in particular to a method for secondary fruiting of phlebopus portentosus, which comprises the following steps: (1) mycelium stimulation; (2) punching; (3) adding a nutritional supplement; (4) earthing; and (5) fruiting management. According to the method, the culture medium after primary fruiting can be further converted and utilized, the yield is greatly increased, and the production cost is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of edible fungi, and in particular to a method for secondary fruiting of dark brown-veined boletus. Background Art

[0002] Phlebopus portentosus, also known as dark brown web-stalked boletus, commonly known as black boletus, belongs to the Boletales, Boletinellaceae, and Phlebopus. Phlebopus is a traditional wild mushroom ingredient with unique flavor in southern Yunnan Province, especially in Xishuangbanna. It does not rot even after long cooking, and always keeps its meat crispy, tender and smooth, with excellent taste. At present, this species has been successfully cultivated artificially and can be mass-produced in factories.

[0003] In the current artificial cultivation of Boletus edulis, the production process is mostly terminated after one fruiting. In view of the low bioconversion rate of Boletus edulis itself, the culture medium after one fruiting is not fully utilized, and there are still many nutrients remaining. Therefore, in the artificial cultivation of Boletus edulis, how to make high-value use of the culture medium after one fruiting is a topic worthy of in-depth research, and it is also an important way to achieve further cost reduction and efficiency improvement in its large-scale mass production. Summary of the invention

[0004] In view of the above technical problems, the present invention provides a method for secondary fruiting of dark brown-veined boletus, which can further transform and utilize the culture medium after primary fruiting, greatly improve the yield, and reduce the production cost.

[0005] In order to achieve the above object, the present invention mainly provides the following technical solutions:

[0006] A method for secondary fruiting of dark brown-veined boletus, the method comprising the following steps:

[0007] (1) Scratching bacteria;

[0008] (2) Punching;

[0009] (3) Add nutritional supplements;

[0010] (4) Covering with soil;

[0011] (5) Mushroom production management.

[0012] Specifically, the second fruiting operation is carried out using the dark brown-veined boletus mushroom bottle or mushroom bag after the first fruiting.

[0013] In step (1), the covering soil layer in the mushroom bottle or bag of the dark brown-veined boletus after the mushrooms have been produced once and the surface fungus skin of the cultivation substrate are removed by scratching the fungi.

[0014] In step (2), 3 to 5 holes are punched in the cultivation substrate with the surface fungus skin removed, and the hole diameter is 0.5 to 0.8 cm.

[0015] In step (3), nutritional supplements are added to the perforated cultivation substrate, and the amount of nutritional supplements added is: 0.5-1g of xylan, 0.1-0.3g of yeast extract, 0.1-0.3g of potassium dihydrogen phosphate, 0.1-0.3g of magnesium sulfate, 0.1-0.2g of xylanase, 0.05-0.1g of amylase and 50-100ml of water per 1kg of cultivation substrate.

[0016] In step (4), soil is covered on the cultivation substrate supplemented with the nutritional supplement, and a mixed bacterial agent is added to the soil covering material used. The soil covering material in this step is an unused soil covering material, which is a conventional soil covering material for cultivating dark brown veined boletus, and can be purchased commercially.

[0017] Specifically, the mixed bacterial agent added to the covering material is obtained by using the covering material of the mushroom bottle or mushroom bag after the mushrooms have grown once, and culturing the soil with bacteria.

[0018] The bacterial culture is specifically as follows: selecting the soil covering material of the mushroom bottle or mushroom bag after the mushrooms have been grown once, transferring it to the bacterial LB culture medium, culturing it at pH 7.2 and 32° C. for 2 days to obtain a mixed bacterial agent.

[0019] The components of the bacterial LB culture medium are: 10 g / L peptone, 5 g / L yeast powder, and 10 g / L NaCl.

[0020] In step (5), the mushroom production management is carried out in stages: in the first stage, the carbon dioxide concentration in the culture environment is 800-1500 ppm, and the culture is carried out under dim light until the mycelium covers the soil layer; in the second stage, the carbon dioxide concentration in the culture environment is 300-800 ppm, and the culture is carried out under bright light to stimulate mushroom production.

[0021] The invention uses the mushroom bottles or mushroom bags of dark brown-veined boletus after primary mushroom production, and obtains dark brown-veined boletus fruiting bodies for secondary mushroom production after the steps of scratching, punching, adding nutrient supplements, covering with soil and mushroom production management, further utilizes the culture medium after primary mushroom production, increases the yield, saves the cost of cultivation materials, and greatly improves the production efficiency. DETAILED DESCRIPTION

[0022] The technical solution of the present invention is described in detail below in conjunction with embodiments.

[0023] Example 1 Comparison of the effects of different nutritional supplements

[0024] Use the dark brown veined boletus bottle after the first fruiting to carry out the second fruiting operation:

[0025] (1) Remove the soil layer in the mushroom bottle and the fungus skin on the surface of the cultivation medium by scratching the fungus.

[0026] (2) After the first fruiting, three holes with a diameter of 0.6 cm were punched in the culture medium of the mushroom bottle.

[0027] (3) The following different nutritional supplements were added to each 1kg of heavy mushroom bottle matrix after the first fruiting, and a comparative test was conducted:

[0028] Supplement ①: xylan 0.5g, yeast extract 0.1g, potassium dihydrogen phosphate 0.1g, magnesium sulfate 0.1g, xylanase 0.1g, amylase 0.05g, water 100ml;

[0029] Supplement ②: xylan 1g, yeast extract 0.3g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.3g, xylanase 0.2g, amylase 0.1g, water 50ml;

[0030] Supplement ③: yeast extract 0.3g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.3g, water 50ml;

[0031] Control: 50 ml of water;

[0032] (4) Adding a mixed bacterial agent to the covering material and covering the soil. The mixed bacterial agent is obtained by using the covering material of the mushroom bottle or mushroom bag after the mushroom is grown once, and then culturing the mixture with bacteria. The bacterial culture is specifically as follows: selecting the covering material of the mushroom bottle or mushroom bag after the mushroom is grown once, transferring it to the bacterial LB culture medium, culturing at pH 7.2 and 32°C for 2 days to obtain the mixed bacterial agent. The components of the bacterial LB culture medium are: peptone 10g / L, yeast powder 5g / L, NaCl 10g / L.

[0033] (5) After covering with soil, mushroom production management is carried out in stages: in the first stage, the carbon dioxide concentration in the culture environment is 1200ppm, and the culture is carried out under dim light until the mycelium covers the soil layer; in the second stage, the carbon dioxide concentration in the culture environment is 500ppm, and the culture is carried out under bright light to stimulate mushroom production.

[0034] (6) Statistics:

[0035] Table 1 Effects of different nutritional supplements on secondary fruiting

[0036]

[0037] As can be seen from the above table, nutritional supplements ① and ② can effectively increase the average mushroom weight in the secondary fruiting of Boletus edulis, and the fruiting rate reached more than 94.5%.

[0038] Example 2 Effect of adding mixed bacterial agent

[0039] Use the dark brown veined boletus bottle after the first fruiting to carry out the second fruiting operation:

[0040] (1) Remove the soil layer in the mushroom bottle and the fungus skin on the surface of the cultivation medium by scratching the fungus.

[0041] (2) Punch three holes in the substrate of the fungus bottle after the first fruiting, with a hole diameter of 0.6 cm.

[0042] (3) The following different nutritional supplements were added to each 1 kg of the heavy mushroom bottle substrate after the first fruiting, and two treatments were set:

[0043] Supplement ①: xylan 0.5g, yeast extract 0.1g, potassium dihydrogen phosphate 0.1g, magnesium sulfate 0.1g, xylanase 0.1g, amylase 0.05g, water 100ml;

[0044] Supplement ②: xylan 1g, yeast extract 0.3g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.3g, xylanase 0.2g, amylase 0.1g, water 50ml;

[0045] (4) Preparation of mixed bacterial agent for covering soil:

[0046] Select the mushroom bottles with higher yield in one mushroom production, sample the covering materials, sample 5g from each bottle, transfer the sample to bacterial LB medium (g / L): peptone 10, yeast powder 5, NaCl 10, add water to make the volume 1L, pH 7.2, and culture at 32℃ for 2 days to obtain the mixed bacterial agent.

[0047] The mixed bacterial agent was added to the soil covering material at 5 ml / bottle and then covered with soil. Meanwhile, a soil covering material without the mixed bacterial agent was provided for comparison.

[0048] (5) After covering with soil, mushroom production management is carried out in stages: in the first stage, the carbon dioxide concentration in the culture environment is 1200ppm, and the culture is carried out under dim light until the mycelium covers the soil layer; in the second stage, the carbon dioxide concentration in the culture environment is 500ppm, and the culture is carried out under bright light to stimulate mushroom production.

[0049] (6) Statistics:

[0050] Table 2 Effect of adding mixed fungi on secondary fruiting

[0051]

[0052] It can be seen from the above table that by adding soil-covering mixed bacterial agents, the mushroom yield can be effectively increased, thereby increasing the yield.

[0053] The process parameters (such as temperature, time, etc.) of the present invention can realize the method by taking upper and lower limits and interval values, and the embodiments are not listed one by one here.

[0054] Any contents not described in detail in the present invention can be based on the conventional technical knowledge in the art.

[0055] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the embodiments, it should be understood by those skilled in the art that any modification or equivalent replacement of the technical solutions of the present invention does not depart from the spirit and scope of the technical solutions of the present invention and should be included in the scope of the claims of the present invention.

Claims

1. A method for secondary fruiting of dark brown-veined boletus, the method comprising the following steps: (1) Scratching bacteria; (2) Punching; (3) Add nutritional supplements; (4) Covering with soil; (5) Mushroom production management.

2. The method for secondary fruiting of Boletus edulis according to claim 1, characterized in that: In step (1), the covering soil layer in the mushroom bottle or bag of the dark brown-veined boletus after the mushrooms have been produced once and the surface fungus skin of the cultivation substrate are removed by scratching the fungi.

3. The method for secondary fruiting of Boletus edulis according to claim 1, characterized in that: In step (2), 3 to 5 holes are punched in the cultivation substrate with the surface fungus skin removed, and the hole diameter is 0.5 to 0.8 cm.

4. The method for secondary fruiting of Boletus edulis according to claim 1, characterized in that: In step (3), nutritional supplements are added to the perforated cultivation substrate, and the amount of nutritional supplements added is: 0.5-1g of xylan, 0.1-0.3g of yeast extract, 0.1-0.3g of potassium dihydrogen phosphate, 0.1-0.3g of magnesium sulfate, 0.1-0.2g of xylanase, 0.05-0.1g of amylase and 50-100ml of water per 1kg of cultivation substrate.

5. The method for secondary fruiting of Boletus edulis according to claim 1, characterized in that: In step (4), soil is covered on the cultivation substrate supplemented with the nutritional supplement, and a mixed bacterial agent is added to the soil covering material.

6. The method for secondary fruiting of Boletus edulis according to claim 5, characterized in that: The mixed bacterial agent is obtained by using the covering material of the mushroom bottle or mushroom bag after the mushrooms are produced once, and then culturing the soil through bacteria.

7. The method for secondary fruiting of Boletus edulis according to claim 6, characterized in that: The bacterial culture is specifically as follows: selecting the soil covering material of the mushroom bottle or mushroom bag after the mushrooms have been grown once, transferring it to the bacterial LB culture medium, culturing it at pH 7.2 and 32° C. for 2 days to obtain a mixed bacterial agent.

8. The method for secondary fruiting of Boletus edulis according to claim 7, characterized in that: The components of the bacterial LB culture medium are: 10 g / L peptone, 5 g / L yeast powder, and 10 g / L NaCl.

9. The method for secondary fruiting of Boletus edulis according to claim 1, characterized in that: In step (5), the mushroom production management is carried out in stages: in the first stage, the carbon dioxide concentration in the culture environment is 800-1500 ppm, and the culture is carried out under dim light until the mycelium covers the soil layer; in the second stage, the carbon dioxide concentration in the culture environment is 300-800 ppm, and the culture is carried out under bright light to stimulate mushroom production.

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