Insecticidal composition containing bishydrazide compound and application thereof

Abstract

The invention belongs to the technical field of pesticide disinfestation, and discloses an insecticidal composition containing a bishydrazide compound and application thereof.The insecticidal composition comprises an active ingredient A and an active ingredient B. The active ingredient A is isoflualanam, the active ingredient B is the bishydrazide compound, and the active ingredient A is isoflualanam. The dihydrazide compound is any one of tebufenozide, methoxyfenozide or chromafenozide, and the mass ratio of the active component A to the active component B is (1: 36)-(45: 1). According to the insecticidal composition disclosed by the invention, insecticides with different action mechanisms are reasonably combined, so that the obtained composition has an enhanced control effect, the insecticidal spectrum is expanded, the rapid action and the long-acting action of pest control are improved, and the generation of drug resistance is favorably controlled or overcome.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide insecticides and discloses an insecticide composition containing a dihydrazide compound and application thereof. Background Art

[0002] Bisaccharides are ecdysone receptor agonists. According to the classification of the Insecticide Resistance Working Committee (IRAC), they are classified as insect growth regulators (IGRs) along with five other types of insecticides, including juvenile hormone analogs, chitin synthesis inhibitors, dipteran insect molting disruptors, and acetyl-CoA carboxylase inhibitors. IGRs are different from other traditional neurotropic insecticides in that they mainly simulate the effects of ecdysone to cause larvae to enter the molting stage in advance, thereby stopping feeding and eventually causing the larvae to die, thereby achieving the purpose of prevention and control. Bisaccharides have gradually become the preferred agent for integrated pest management due to their high efficiency, low toxicity, low residue, and safety to the environment and non-target organisms.

[0003] In recent years, the prohibition of highly toxic pesticides and the increasing insect resistance have caused the previous chemical agents to significantly reduce or even fail to prevent diseases and insect pests, so the prevention and control of pests has become increasingly difficult. Therefore, finding low-toxic and high-efficiency new pesticides and controlling insect resistance have become a hot spot in pesticide research and development. The present invention rationally compounds dihydrazide pesticides with isoflualanam, studies the field control effect of the active agent after the combination on lepidopteran pests, and provides a theoretical basis for scientific and rational use of drugs. Summary of the invention

[0004] In order to solve the above problems existing in the prior art, the present invention provides an insecticide composition containing a dihydrazide compound and its application. The insecticide composition has excellent control effect on agricultural lepidopteran pests, reduces the dosage of pesticides used, and slows down the generation of pest resistance.

[0005] In order to achieve the above object, the present invention adopts the following technical scheme: an insecticidal composition containing a hydrazide compound, the insecticidal composition comprises an active ingredient A and an active ingredient B, the active ingredient A is isoflualanam, the active ingredient B is a dihydrazide compound, the dihydrazide compound is any one of fenpropizolin, methoxyfenopyrazide or chromafenopyrazide, and the mass ratio of the active ingredient A to the active ingredient B is 1:36 to 45:1, or any value within the above numerical range.

[0006] Furthermore, the active ingredient B is tebufenozide, and the mass ratio of isoflualanam to tebufenozide is 1:25 to 35:1, or any value within the above numerical range;

[0007] The active ingredient B is methoxyfenozide, and the mass ratio of isoflualanam to methoxyfenozide is 1:25 to 30:1, or any value within the above numerical range;

[0008] The active ingredient B is chlorfenapyr, and the mass ratio of isoflualanam to chlorfenapyr is 1:30 to 20:1, or any value within the above numerical range.

[0009] Furthermore, the active ingredient B is tebufenozide, and the mass ratio of isoflualanam to tebufenozide is 1:25 to 22:1, or any value within the above numerical range;

[0010] The active ingredient B is methoxyfenozide, and the mass ratio of isoflualanam to methoxyfenozide is 1:15 to 30:1, or any value within the above numerical range;

[0011] The active ingredient B is chlorfenapyr, and the mass ratio of isoflualanam to chlorfenapyr is 1:22 to 20:1, or any value within the above numerical range.

[0012] Furthermore, the active ingredient B is tebufenozide, and the mass ratio of isoflualanam to tebufenozide is 1:20 to 18:1, or any value within the above numerical range;

[0013] The active ingredient B is methoxyfenozide, and the mass ratio of isoflualanam to methoxyfenozide is 1:15 to 20:1, or any value within the above numerical range;

[0014] The active ingredient B is chlorfenapyr, and the mass ratio of isoflualanam to chlorfenapyr is 1:14 to 15:1, or any value within the above numerical range.

[0015] Furthermore, the total weight of the insecticide composition is calculated as 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the insecticide composition, or any value within the above numerical range.

[0016] Furthermore, the insecticide composition contains, in addition to the active ingredients, auxiliary ingredients permitted in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

[0017] Furthermore, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0018] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0019] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0020] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0021] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0022] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0023] The defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0024] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0025] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0026] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0027] The synergist is selected from synergist phosphite, synergist ether; and / or

[0028] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0029] Furthermore, the insecticide composition is prepared into a formulation form permitted by pesticides, and the formulation form is a solid formulation or a liquid formulation;

[0030] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0031] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions;

[0032] Furthermore, the solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension, an emulsifiable concentrate, an aqueous emulsion, a microemulsion or a dispersible oil suspension.

[0033] The invention also discloses the use of the insecticide composition and its preparation for preventing and controlling agricultural pests.

[0034] Furthermore, the pests are lepidopteran pests;

[0035] Furthermore, the Lepidoptera includes, but is not limited to, Plutellaxylostella, diamondback moth, Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays oleae (olive moth), Pseudaletia spp. (snoctuids), Pseudaletia unipunctata (armyworm), Pseudoplusia includes (soybean looper), Rachiplusia nu, Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia inferens (pink rice stem borer), Sesamia spp. (stem borers), nonagrioides), Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp. (snooper moth), Spodoptera exigua (beetarmyworm), Spodoptera fugiperda (fall armyworm), Adoxophyes spp., Adoxophyes orana, Agrotis spp.) (cutworm), Agrotis ipsilon (black cutworm), Alabamaargillacea, Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anarsia lineatella (peach twig borer), Anomissa bulifera (jute looper), Anticarsia gemmata lis (velvet bean pilla r), Archips argyrospila (fruittree leafroller), Archips rosana (rose leaf roller), Argyrotaenia spp. (tortricid moths), Argyrotaenia citrana, Autographa gamma, Bonagota cranaodes, Borbo cinnara), Caloptilia spp. (leaf miners), Capuareticulana, Carposina niponensis (peach fruit moth), Chilospp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded leafroller), Chrysodeixis spp., Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus, Crambus spp.) (Sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella, Darna diducta, Diaphania spp. (stem borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugar cane borer), Diatraea graniosella (southwester cornborer), Earias spp. (cotton bollworms), Earias spp. (Earias spp.) insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopophaaurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Ephestia spp. (flour moths), Ephestia cautella (almond moth), Ephestia elutella (tobbaco moth), Ephestia kuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella, Euxoa auxiliaris (army cutworm), Feltia spp., Gortyna spp.) (stem borers), Grapholita molesta (oriental fruit moth), Hedylepta indicate, bean leaf webber, Helicoverpas p p. (spotworms), Helicover paarmigera, Helicoverpa zea, Heliothis spp. (spotworms), Heliothis virescens, tobacco budworm, Hellula undalis (cabbage webworm), Indarbela spp., root borers, Keiferia lycopersicella, (tomato pinworm, Leucinodes orbonalis (eggplant fruit borer) borer), Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruit moth), Loxagrotis spp. (south armyworm), Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp.)(tent caterpillars), Mamestra brassicae (cabbagearmyworm), Maruca testulalis, Metisa plana, Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis (small tomato borer), Operophthera brumata (winter moth), Ostrinia nubilalis (European corn borer), Pandemis cerasana, common curranttortrix, Pandemis heparana (brown apple tortrix), Papilio demodocus, Pectinophora gossypiella (pink bollworm), Peridroma species (Peridroma spp.) (cutworm), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potatotuber moth), Phyllocnisitis citrella, Phyllonorycter spp. (leafminers), Pieris rapae (imported cabbageworm), Plathypena scabra, Plodia interpunctella (Indian mealmoth), Spodoptera oridania (southern armyworm), Synanthedon spp.)(root borers), Thecla basilides, Thermisiagemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusia ni (pigworm), Tuta absoluta, Yponomeuta spp., Zeuzera coffeae (red branch borer), and Zeuzera pyrina (leopard moth).

[0036] In particular, the insecticide composition of the present invention has excellent control effects on diamondback moth, beet armyworm and rice borer.

[0037] Furthermore, the insecticide composition or its preparation is applied to the pests to be controlled or the medium in which they grow.

[0038] In order to obtain the desired insecticidal effect, the dosage of the insecticidal composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application site, application method, dosage form used, etc.

[0039] The beneficial effects of the present invention are as follows:

[0040] The insecticide composition of the invention is compounded with isoflualanam and dihydrazide compounds having different action mechanisms, and has obvious synergistic effect on lepidopteran pests within a suitable ratio, and has remarkable control effect, and reduces the dosage of the pesticide and slows down the development of pest resistance. DETAILED DESCRIPTION

[0041] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0042] Preparation Example:

[0043] Preparation Example 1: 20% isoflualanam·tebufenozide suspension (2:3)

[0044] Formula: 8% isoflualanam, 12% tebufenozide, 1% sodium lauryl sulfate, 2% sodium alkyl polyoxyethylene ether sulfonate, 3% polyoxyethylene sorbitan monooleate, 3% tristyrylphenol ethoxylate phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 4% propylene glycol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0045] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0046] Preparation Example 2: 6% isoflualanam·tebufenozide EC (5:1)

[0047] Formula: 5% isoflualanam, 1% tebufenozide, 10% dimethyl sulfoxide, 10% tristyrylphenol polyoxyethylene ether, 2% calcium dodecylbenzene sulfonate, 25% propylene carbonate, and xylene to make up the balance;

[0048] Preparation method: add the measured active ingredients, solvents and co-solvents into a mixing kettle and stir to dissolve them, then add an emulsifier, make up the balance with the remaining solvent, stir evenly in a stirring kettle, and filter to obtain the desired emulsifiable concentrate of the present invention.

[0049] Preparation Example 3: 30% isoflualanam·tebufenozide wettable powder (1:5)

[0050] Formula: 5% isoflualanam, 25% tebufenozide, 2% sodium dodecyl sulfate, 3% sodium lignin sulfonate, 4% alkyl naphthalene sulfonate, 6% naphthalene sulfonate formaldehyde condensate, 15% kaolin, 10% white carbon black, and bentonite makes up the balance;

[0051] Preparation method: The active ingredients, dispersants, wetting agents and fillers are mixed according to the formula ratio, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly for multiple times by a jet mill to prepare the wettable powder of the composition of the present invention.

[0052] Preparation Example 4: 20% isoflualanam·methoxyfenozide suspension (4:1)

[0053] Formula: 16% isoflualanam, 4% methoxyfenozide, 1% triphenylethylphenol polyoxyethylene ether, 5% arylphenol polyoxyethylene ether phosphate, 1% naphthalenesulfonate formaldehyde condensate, 0.25% xanthan gum, 5% ethylene glycol, 0.1% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0054] Preparation method: Same as Preparation Example 1.

[0055] Preparation Example 5: 3% isoflualanam·methoxyfenozide emulsifiable concentrate (2:1)

[0056] Formula: 2% isoflualanam, 1% methoxyfenozide, 22% propylene glycol methyl ether, 12% ethylene glycol oxyethylene polyoxypropylene ether, 2% calcium dodecylbenzene sulfonate, 12% DMF, and methyl oleate makes up the balance.

[0057] Preparation method: Same as Preparation Example 2.

[0058] Preparation Example 6: 36% isoflualanam·methoxyfenozide wettable powder (1:8)

[0059] Formula: 4% isoflualanam, 32% methoxyfenozide, 5% sodium polycarboxylate, 2% sodium alkyl polyoxyethylene ether sulfonate, 2% BX powder, 7% white carbon black, and kaolin to make up the balance;

[0060] Preparation method: Same as Preparation Example 3.

[0061] Preparation Example 7: 16% isoflualanam·chloranil hydrazide suspension (7:1)

[0062] Formula: 14% isoflualanam, 2% chlorfenapyr, 2% anhydrous sorbitan polyoxyethylene ether, 3% isomeric tridecanol polyoxyethylene ether, 2% styrenated phenol polyoxyethylene ether phosphate, 2% polycarboxylic acid sodium salt, 1.5% magnesium aluminum silicate, 0.15% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0063] Preparation method: Same as Preparation Example 1.

[0064] Preparation Example 8: 9% isoflualanam·chlorfenapyr EC (5:4)

[0065] Formula: 5% isoflualanam, 4% chlorfenapyr, 12% EO / PO block copolymer, 12% acetophenone, 10% propylene glycol methyl ether, 3% calcium dodecylbenzene sulfonate, and trimethylbenzene makes up the balance;

[0066] Preparation method: Same as Preparation Example 2.

[0067] Preparation Example 9: 36% isoflualanam·chlorfenapyr wettable powder (2:7)

[0068] Formula: 8% isoflualanam, 28% chlorfenapyr, 2% sodium dodecyl sulfate, 2% naphthalene sulfonate formaldehyde condensate, 5% alkyl naphthalene sulfonate, 5% fatty alcohol polyoxyethylene ether sulfate, 10% kaolin, 8% white carbon black, and bentonite makes up the balance;

[0069] Preparation method: Same as Preparation Example 3.

[0070] Example 1: Indoor biological activity test on beet armyworm

[0071] Test basis: The test refers to NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 14 - Leaf Dipping Method".

[0072] Test target: 3rd instar larvae of Spodoptera exigua.

[0073] Experimental agents: isoflualanam, fenbumid, methoxyfenbumid, and chlorfenapyr technical drugs.

[0074] Test method: Select sensitive test insects that are raised indoors and of the same age. Dissolve the above original drugs with a suitable solvent, and then dilute with an aqueous solution containing 0.1% Tween-80. According to the activity of the agent, set 5 mass concentration gradients according to the proportional method. Select fresh cabbage leaves that have not been used with drugs, and use a hole puncher to make leaf discs. After dipping the leaf disc in the test solution for 10 seconds, take it out and dry it. Place it in a culture dish containing moisturizing filter paper and inoculate the test insects. The treated test insects are raised under the conditions of a temperature of (26±1)℃, a relative humidity of 65%±5%, and a light cycle of L:D=14h:10h. Each treatment is repeated 4 times, and 20 insects are immersed in each repetition. A treatment with a corresponding organic solvent without drugs is set as a blank control.

[0075] Experimental investigation: Investigate the mortality of test insects 72 hours after treatment and record the total number of insects and the number of dead insects.

[0076] Calculation method:

[0077] According to the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0078]

[0079] Where:

[0080] P——mortality rate, in percentage (%);

[0081] K——indicates the number of dead insects, in units of heads;

[0082] N——represents the total number of insects treated, in heads.

[0083]

[0084] Where:

[0085] P1——adjusted mortality rate, in percentage (%);

[0086] P t ——Treatment mortality rate, expressed as percentage (%);

[0087] P0——blank control mortality rate, in percentage (%).

[0088] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0089] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50 Value, to evaluate the activity of the test agent on the biological test material.

[0090] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0091]

[0092] Where:

[0093] ATI——Actual Toxicity Index of Mixture;

[0094] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0095] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0096] TTI=TI A ×P A +TI B ×P B

[0097] Where:

[0098] TTI – Theoretical Toxicity Index of Mixtures;

[0099] TI A ——A: toxicity index of the agent;

[0100] P A ——The percentage of agent A in the mixture, in percentage (%);

[0101] TI B ——Toxicity index of agent B;

[0102] P B——The percentage of agent B in the mixture, in percentage (%).

[0103]

[0104] Where:

[0105] CTC – Co-toxicity coefficient;

[0106] ATI——Actual Toxicity Index of Mixture;

[0107] TTI - Theoretical Toxicity Index of Mixture.

[0108] The co-toxicity coefficient of the combination CTC ≥ 120 shows a synergistic effect; CTC ≤ 80 shows an antagonistic effect; 80 < CTC < 120 shows an additive effect.

[0109] The indoor test results are shown in the following table:

[0110] Table 1 Results of the indoor biological activity test of isoflualanam and tebufenozide on beet armyworm

[0111]

[0112]

[0113] The results of the indoor test showed that the compounding of isoflualanam and tebufenozide in an appropriate mass ratio showed a synergistic effect on the beet armyworm within a certain ratio range. When the mass ratio of isoflualanam to tebufenozide was 1:25-22:1, the co-toxicity coefficient to the beet armyworm was greater than 120, showing a synergistic effect; when the mass ratio of isoflualanam to tebufenozide was 1:20-18:1, the co-toxicity coefficient to the beet armyworm was greater than 130, showing a significant synergistic effect; when the mass ratio of isoflualanam to tebufenozide was 1:10-12:1, the co-toxicity coefficient was greater than 140, showing a significant synergistic effect.

[0114] Table 2 Results of the indoor biological activity test of isoflualanam and methoxyfenozide on beet armyworm

[0115]

[0116] The results of the indoor test showed that the compounding of isoflualanam and methoxyfenozide in an appropriate mass ratio showed a synergistic effect on the beet armyworm. When the mass ratio of isoflualanam to methoxyfenozide was 1:25-30:1, the co-toxicity coefficient against the beet armyworm was greater than 120, showing a synergistic effect; when the mass ratio of isoflualanam to methoxyfenozide was 1:15-30:1, the co-toxicity coefficient against the beet armyworm was greater than 130, showing a significant synergistic effect; when the mass ratio of isoflualanam to methoxyfenozide was 1:15-20:1, the co-toxicity coefficient against the beet armyworm was greater than 140, showing a significant synergistic effect.

[0117] Table 3 Results of the indoor biological activity test of isoflualanam and chlorfenapyr combined with beet armyworm

[0118]

[0119]

[0120] The results of the indoor test showed that the compounding of isoflualanam and chlorfenapyr in an appropriate mass ratio showed a synergistic effect on the beet armyworm. When the mass ratio of isoflualanam to chlorfenapyr was 1:30-20:1, the co-toxicity coefficient against the beet armyworm was greater than 120, showing a synergistic effect; when the mass ratio of isoflualanam to chlorfenapyr was 1:22-15:1, the co-toxicity coefficient against the beet armyworm was greater than 140, showing a significant synergistic effect.

[0121] Example 2: Indoor biological activity test on rice stem borer

[0122] Test basis: The test refers to NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 14 - Leaf Dipping Method".

[0123] Test target: 3rd instar larvae of Chilo suppressalis.

[0124] Experimental agents: isoflualanam, fenbumid, methoxyfenbumid, and chlorfenapyr technical drugs.

[0125] Test method: Select sensitive test insects that are reared indoors and of the same age. Dissolve the above original drugs with a suitable solvent and dilute with a 0.1% Tween-80 aqueous solution. According to the activity of the drug, set 5 mass concentration gradients according to the equal ratio method. Select the upper leaves of fresh tillering rice that have not been treated with drugs, about 10 cm, immerse them in the drug solution for 30 seconds, and after air drying on filter paper, spread about 20 rice leaves flat in a 20cm germination box, and use a soft brush to gently transfer the 3rd instar larvae of rice leaf roller to the germination box. There are 20 test insects in each germination box. Cover the germination box with plastic wrap and pierce it with a toothpick for ventilation. The treated test insects are placed at a temperature of (26±1)℃, a relative humidity of 65%±5%, and a light cycle of L:D=14h:10h. Each treatment is repeated 4 times, and 20 insects are immersed in each repeat. The corresponding organic solvent treatment without the drug is set as a blank control. The mortality of larvae was observed after 48 hours. No reaction to needle prick or black and shrunken larvae were regarded as the mortality standard of rice leaf folder larvae.

[0126] Calculation method:

[0127] According to the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0128]

[0129] Where:

[0130] P——mortality rate, in percentage (%);

[0131] K——indicates the number of dead insects, in units of heads;

[0132] N——represents the total number of insects treated, in heads.

[0133]

[0134] Where:

[0135] P1——adjusted mortality rate, in percentage (%);

[0136] P t ——Treatment mortality rate, expressed as percentage (%);

[0137] P0——blank control mortality rate, in percentage (%).

[0138] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0139] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50Value, to evaluate the activity of the test agent on the biological test material.

[0140] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0141]

[0142] Where:

[0143] ATI——Actual Toxicity Index of Mixture;

[0144] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0145] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0146] TTI=TI A ×P A +TI B ×P B

[0147] Where:

[0148] TTI – Theoretical Toxicity Index of Mixtures;

[0149] TI A ——A: toxicity index of the agent;

[0150] P A ——The percentage of agent A in the mixture, in percentage (%);

[0151] TI B ——Toxicity index of agent B;

[0152] P B ——The percentage of agent B in the mixture, in percentage (%).

[0153]

[0154] Where:

[0155] CTC – Co-toxicity coefficient;

[0156] ATI——Actual Toxicity Index of Mixture;

[0157] TTI - Theoretical Toxicity Index of Mixture.

[0158] The co-toxicity coefficient of the combination CTC ≥ 120 shows a synergistic effect; CTC ≤ 80 shows an antagonistic effect; 80 < CTC < 120 shows an additive effect.

[0159] The indoor test results are shown in the following table:

[0160] Table 4 Results of the indoor biological activity test on isoflualanam and tebufenozide against rice stem borer

[0161]

[0162]

[0163] The results of the indoor test showed that the compounding of isoflualanam and tebufenozide in an appropriate mass ratio showed a synergistic effect on the rice stem borer within a certain ratio range. When the mass ratio of isoflualanam to tebufenozide was 1:25-35:1, the co-toxicity coefficient to the rice stem borer was greater than 120, showing a synergistic effect; when the mass ratio of isoflualanam to tebufenozide was 1:18-24:1, the co-toxicity coefficient to the rice stem borer was greater than 130, showing a significant synergistic effect; when the mass ratio of isoflualanam to tebufenozide was 1:18-16:1, the co-toxicity coefficient was greater than 140, showing a significant synergistic effect.

[0164] Table 5 Results of the indoor biological activity test on isoflualanam and methoxyfenozide against rice stem borer

[0165]

[0166] The results of the indoor test showed that the compounding of isoflualanam and methoxyfenozide in an appropriate mass ratio showed a synergistic effect on the rice stem borer. When the mass ratio of isoflualanam to methoxyfenozide was 1:24-30:1, the co-toxicity coefficient to the rice stem borer was greater than 120, showing a synergistic effect; when the mass ratio of isoflualanam to methoxyfenozide was 1:16-16:1, the co-toxicity coefficient to the rice stem borer was greater than 130, showing a significant synergistic effect; when the mass ratio of isoflualanam to methoxyfenozide was 1:8-16:1, the co-toxicity coefficient to the rice stem borer was greater than 140, showing a significant synergistic effect.

[0167] Table 6 Results of the indoor biological activity test on isoflualanam and chlorfenapyr against rice stem borer

[0168]

[0169]

[0170] The results of the indoor test showed that the compounding of isoflualanam and chlorfenapyr in an appropriate mass ratio showed a synergistic effect on the rice stem borer. When the mass ratio of isoflualanam to chlorfenapyr was 1:36-45:1, the co-toxicity coefficient to the rice stem borer was greater than 120, showing a synergistic effect; when the mass ratio of isoflualanam to chlorfenapyr was 1:25-32:1, the co-toxicity coefficient to the rice stem borer was greater than 130, showing a significant synergistic effect; when the mass ratio of isoflualanam to chlorfenapyr was 1:20-28:1, the co-toxicity coefficient to the rice stem borer was greater than 140, showing a significant synergistic effect.

[0171] Example 3: Field test on efficacy of drugs against beet armyworm

[0172] Experimental location: The experiment was arranged in Sunjiazhai Village, Xiaodian District, Taiyuan City, Shanxi Province. The soil of the experimental site is sandy loam with medium fertility. The cultivation and management of the experimental site are in line with local scientific agricultural practices.

[0173] Test target: Beet armyworm.

[0174] Experimental crops: Cabbage.

[0175] Experimental design: The experiment set up 8 treatments, each treatment was repeated 4 times, and each plot was 15m 2 All experimental plots were arranged in random blocks.

[0176] Test method: The application time is the early stage of beet armyworm outbreak, the pesticide is mixed with water for foliar spraying, and the water consumption is 750kg / hm 2 The spray should be evenly and carefully applied on both sides of the cabbage leaves, so that the leaves are wet but not dripping. For the blank treatment, spray an equal amount of clean water.

[0177] Survey method: fixed-point survey, sampling at 5 points in each plot, 10 plants at each point, and a total of 50 plants in each plot. Investigate the insect population base before spraying, and investigate the number of residual insects 2, 5, and 10 days after spraying.

[0178] Calculation method of drug efficacy:

[0179]

[0180] Field efficacy test results:

[0181] Table 7 Results of field efficacy test on control of beet armyworm

[0182]

[0183]

[0184] It can be seen from the field efficacy test in the above table that the combination of isoflualanam and dihydrazide compounds showed good control effect on beet armyworm. Ten days after application, the control effect of the combined preparation on beet armyworm was 89.86% to 93.25%.

[0185] Example 4: Field test on efficacy of controlling rice stem borer

[0186] Test location: rice transplanting field in Liguan Village, Jiyang District, Jinan City, Shandong Province. The previous crop of the test site was wheat. The test site is flat and the soil fertility of each test plot is uniform.

[0187] Test target: Rice stem borer.

[0188] Experimental design: There are 8 treatments in the experiment, and the area of ​​each plot is 30m 2 All experimental plots were arranged in random blocks, and isolation zones were set between the plots.

[0189] Test method: When the rice stem borer reached the peak of egg hatching, the pesticide was applied using a 3WBJ-16DZ multi-functional backpack electric sprayer at a rate of 1.5 times per 667 m 2 Add 30L of water and spray evenly, the water consumption is 450L / hm 2 There should be no rainfall on the day of application and within 3 days after application, and water should be retained for 1 week after application.

[0190] Efficacy investigation: 20 days after application, when the damage in the control area is obvious or the contemporary damage is finalized, parallel jumping sampling is carried out at 10 points, with 5 clumps at each point and a total of 50 clumps in each plot. The total number of plants and the number of dead cores are recorded, and the dead core rate and control effect are calculated.

[0191] Calculation method of drug efficacy:

[0192]

[0193] Field efficacy test results:

[0194] Table 8 Field efficacy test on control of rice stem borer

[0195]

[0196]

[0197] The results of the field efficacy test showed that the mixture of isoflualanam and dihydrazide compounds had a good control effect on the rice stem borer; 20 days after the application, the comprehensive control effect of the compound preparation on the rice stem borer was above 88%, showing better persistence compared with the control single agent.

[0198] Although the application describes specific embodiments in detail by means of examples, the disclosure of the application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the application is not limited to the disclosed specific forms. On the contrary, the disclosure of the application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the application, and the scope of the application is limited by the attached claims and their legal equivalents.

Claims

1. An insecticidal composition containing a dihydrazide compound, characterized in that: The insecticide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is isoflualanam, and the active ingredient B is a dihydrazide compound, wherein the dihydrazide compound is any one of fenpropizolin, methoxyfenoporozide or chromafenoporozide, and the mass ratio of the active ingredient A to the active ingredient B is 1:36 to 45:

1.

2. The insecticidal composition according to claim 1, characterized in that The active ingredient B is tebufenozide, and the mass ratio of isoflualanam to tebufenozide is 1:25 to 35:1; The active ingredient B is methoxyfenozide, and the mass ratio of isoflualanam to methoxyfenozide is 1:25 to 30:1; The active ingredient B is chlorfenapyr, and the mass ratio of isoflualanam to chlorfenapyr is 1:30 to 20:

1.

3. The insecticidal composition according to claim 2, characterized in that The active ingredient B is tebufenozide, and the mass ratio of isoflualanam to tebufenozide is 1:25 to 22:1; The active ingredient B is methoxyfenozide, and the mass ratio of isoflualanam to methoxyfenozide is 1:15 to 30:1; The active ingredient B is chlorfenapyr, and the mass ratio of isoflualanam to chlorfenapyr is 1:22 to 20:

1.

4. The insecticidal composition according to claim 3, characterized in that The active ingredient B is tebufenozide, and the mass ratio of isoflualanam to tebufenozide is 1:20 to 18:1; The active ingredient B is methoxyfenozide, and the mass ratio of isoflualanam to methoxyfenozide is 1:15 to 20:1; The active ingredient B is chlorfenapyr, and the mass ratio of isoflualanam to chlorfenapyr is 1:14-15:

1.

5. The insecticidal composition according to claim 1, characterized in that The total weight of the insecticide composition is calculated as 100 wt %, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the insecticide composition.

6. The insecticidal composition according to claim 1, characterized in that The insecticide composition contains, in addition to the active ingredients, auxiliary ingredients permitted in pesticides, wherein the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

7. The insecticidal composition according to claim 1, characterized in that The insecticide composition is prepared into a formulation form permitted by pesticides, and the formulation form is a solid formulation or a liquid formulation.

8. The insecticidal composition according to claim 7, characterized in that The solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension, an emulsifiable concentrate, an aqueous emulsion, a microemulsion or a dispersible oil suspension.

9. Use of the insecticidal composition and the preparation thereof according to any one of claims 1 to 8 for controlling agricultural pests.

10. The use according to claim 9, characterized in that: The pests are lepidoptera pests.

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