Microbial fermented feed suitable for frogs and preparation method of microbial fermented feed

Abstract

The invention relates to the technical field of microbial fermentation feeds, in particular to a microbial fermentation feed suitable for frogs and a preparation method of the microbial fermentation feed. The invention relates to a microbial fermentation feed suitable for frogs and a preparation method thereof. The preparation method comprises the following steps: preparing jerusalem artichoke whole powder; the bacillus is activated; preparing a compound bacterium suspension; carrying out enzymolysis treatment on the fermentation raw materials; performing fermentation; and preparing a microbial fermentation feed finished product. Water-soluble fructo-oligosaccharide, namely the jerusalem artichoke whole powder, is added in the process of preparing the microbial fermentation feed and can serve as prebiotics, the jerusalem artichoke whole powder is added into the feed, the intestinal micro-ecological balance of frogs can be adjusted, the absorption and digestion functions of intestines and stomach are promoted, the immune function is improved, the dissolution rate of the jerusalem artichoke whole powder is very low, and the effect of improving the immunity of the frogs is achieved. And the frogs do not lose too much when eating.

Description

Technical Field

[0001] The invention relates to the technical field of microbial fermentation feed, and in particular to a microbial fermentation feed suitable for frogs and a preparation method thereof. Background Art

[0002] Frogs are a type of frog, commonly known as "frogs". They have high edible value, tender meat, high nutritional value, low fat and low sugar content. They can also clear away heat and detoxify, reduce swelling and relieve pain. They are classified as a national second-class protected animal. In recent years, the demand for frogs has been very large. Due to their strong reproductive capacity and low breeding costs, frogs have now become one of the hottest breeding projects.

[0003] At present, the feeds used for breeding frogs mainly include granular feeds and puffed feeds. Traditional granular feeds are not easy to preserve and are easy to absorb water and deteriorate. In addition, the particle sizes are different and cannot adapt well to the changes in the frog's body shape to adjust the particle size. Traditional puffed feeds are easy to dissolve in water and do not contain more nutrients, and cannot allow frogs to take in various nutrients in a balanced manner. The utilization rate of feed is too low and the economic benefits are not high. Therefore, a new type of microbial fermentation feed for frogs and a preparation method thereof are needed. Summary of the invention

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a microbial fermentation feed suitable for frogs and a preparation method thereof.

[0005] A microbial fermented feed for frogs and a preparation method thereof, specifically comprising the following steps:

[0006] S1: Preparation of Jerusalem artichoke powder

[0007] Wash the mud and sand on the epidermis of fresh Jerusalem artichoke tubers with clean water, peel them, slice the Jerusalem artichokes into Jerusalem artichoke slices with a thickness of 1-3 mm using a slicer, then soak them in a 0.2-0.3% citric acid aqueous solution, dry the Jerusalem artichoke slices in an air drying oven, crush them, and pass them through a 100-300 mesh sieve to obtain Jerusalem artichoke whole powder;

[0008] S2: Activation of Bacillus species

[0009] Use a sterile pipette to inoculate the frozen Bacillus solution into the solid activation medium, put it into an incubator, and culture it at 36-38°C for 22-26 hours. Pick a single colony and inoculate it into the liquid activation medium, and culture it at 36-38°C and 180-220r / min for 43-45 hours. Store it at 3-5°C for later use.

[0010] S3: Preparation of composite bacterial suspension

[0011] The activated bacillus was inoculated into a nutrient agar slant medium, and yeast and lactic acid bacteria solution were added, and the mixture was cultured at 35-37°C for 20-24 hours, and the composite bacteria were washed off with sterile water, and the mixture was heated at 60-80°C for 20-40 minutes to obtain an intermediate bacterial solution I, and the concentration of the intermediate bacterial solution I was adjusted to 100-120 cfu / mL with a diluent, and the mixture was stored at 3-5°C for later use;

[0012] S4: Enzymatic hydrolysis of fermentation raw materials

[0013] Take 15-40 parts of soybean meal, 3-25 parts of corn flour, 1-5 parts of fish meal and 3-10 parts of kelp powder by weight, grind, pass through a 180-220 mesh sieve, add 0.5-1.5 parts of cellulase, 0.8-1.2 parts of protease, and 90-110 parts of water, control the temperature to 50-60° C., the pH to 6-8, treat for 14-16 hours, then heat to 90-110° C., for 8-12 minutes, perform enzyme inactivation treatment, and obtain a treated fermentation raw material;

[0014] S5: Fermentation

[0015] 100-120 parts by weight of the processed fermentation raw materials are taken out, 20-40 parts of Jerusalem artichoke powder and 1.8-3.6 parts of attractants are added, and the mixture is evenly mixed, and the mixture is further mixed with the composite bacterial suspension, and put into a fermentation machine for fermentation for 22-24 hours at a temperature of 36-40° C. and a pH of 6-8, and low-temperature drying is performed at a temperature of 32-34° C. and a moisture mass percentage of 30%-35%, to obtain a primary fermented feed;

[0016] S6: Preparation of finished microbial fermented feed

[0017] Casein and gelatin are added to the primary fermented feed, and after adding a small amount of water to fully mix, the emulsion passing through a 90-110 mesh sieve is added into a sprayer, and it is sprayed into a 4-6% calcium chloride solution under pressure, and the particles are collected, and then placed in a 0.4-0.6% calcium chloride solution and heated for 8-12 minutes at 70-90°C, and then placed in a carbonate solution and soaked for 20-40 minutes, and then washed with pure water and screened to obtain a finished microbial fermented feed.

[0018] Furthermore, it is characterized in that step S2 activates the Bacillus species, specifically comprising the following steps:

[0019] S2.1: Use a sterile pipette to inoculate the frozen Bacillus solution into the solid activation medium, place it in an incubator, and culture it at a constant temperature of 36-38°C for 22-26 hours to obtain a primary activation product;

[0020] S2.2: Pick a single colony and inoculate it into liquid activation culture medium, culture it at 36-38℃ and 180-220r / min for 43-45h to obtain secondary activation product, and store it at 3-5℃ for later use.

[0021] Furthermore, it is characterized in that the activation medium in step S2.1 is LB medium, and the activation medium in step S2.2 is broth medium.

[0022] Furthermore, it is characterized in that the preparation of the S3 composite bacterial suspension specifically comprises the following steps:

[0023] S3.1: Inoculate the Bacillus into the nutrient agar slant medium, add yeast and lactic acid bacteria, culture at 35-37℃ for 20-24h, wash the composite bacteria with sterile water, and heat at 60-80℃ for 20-40min to obtain intermediate bacterial solution I;

[0024] S3.2: Use diluent to adjust the concentration of intermediate bacterial solution I to 100-120 cfu / mL and store at 3-5℃ for later use.

[0025] Furthermore, it is characterized in that the diluent in step S3.2 can be 0.8-1.0% sterile sodium chloride solution, 0.08-0.12% protein peptide aqueous solution and sodium chloride-protein peptide buffer with a pH of 6-8.

[0026] Furthermore, it is characterized in that step S4 performs enzymatic hydrolysis on the fermentation raw material, which specifically comprises the following steps:

[0027] S4.1: Take 15-40 parts by weight of soybean meal, 3-25 parts of corn meal, 1-5 parts of fish meal and 3-10 parts of kelp meal, grind them, and pass them through a 180-220 mesh sieve to obtain a mixture;

[0028] S4.2: adding 0.5-1.5 parts of cellulase, 0.8-1.2 parts of protease, and 90-110 parts of water to the mixture, controlling the temperature to 50-60°C and the pH to 6-8, treating for 14-16 hours, and obtaining a fermentation raw material solution after enzymatic hydrolysis;

[0029] S4.3: The solution is heated to 90-110°C for 8-12 minutes to inactivate the enzyme and obtain the treated fermentation raw material.

[0030] Furthermore, it is characterized in that step S5 is fermented, which specifically includes the following steps:

[0031] S5.1: Take out 100-120 parts by weight of the processed fermented raw materials, add 20-40 parts of chrysanthemum powder and 1.8-3.6 parts of attractant, and mix well;

[0032] S5.2: the mixture is further mixed with the composite bacterial suspension, and placed in a fermentation machine for fermentation for 22-24 hours at a temperature of 36-40°C and a pH of 6-8 to obtain a fermentation intermediate solution I;

[0033] S5.3: The fermentation intermediate solution I is dried at low temperature at a temperature of 32-34° C. and a water content of 30%-35% by mass to obtain primary fermented feed.

[0034] Furthermore, it is characterized in that the attractant in step S5.1 contains 1-3 parts of betaine and 8-15 parts of squid viscera powder.

[0035] Furthermore, it is characterized in that step S6 prepares the finished product of microbial fermentation feed, which specifically comprises the following steps:

[0036] S6.1: Add 2-4 parts of casein and 4-6 parts of gelatin to 96-100 parts of primary fermented feed, add a small amount of water and mix thoroughly, and collect the emulsion that passes through a 90-110 mesh sieve;

[0037] S6.2: Load the emulsion into a sprayer, spray it onto a 4-6% calcium chloride solution at a pressure of 1400-1600 kPa, and collect the particles;

[0038] S6.3: Place the particles in a 0.4-0.6% calcium chloride solution and heat for 8-12 minutes at 70-90°C, then soak them in a carbonate solution for 20-40 minutes, wash them with pure water and select particles of 40-60 mesh to obtain the finished microbial fermented feed.

[0039] The beneficial effects are: 1. The present invention adds a water-soluble oligofructose, namely Jerusalem artichoke powder, in the process of preparing microbial fermentation feed, which can be used as a prebiotic and added to the feed to regulate the intestinal microecological balance of frogs, promote gastrointestinal absorption and digestion function and improve the immune function. The dissolution rate of Jerusalem artichoke powder is very low, and the frog will not lose too much when eating.

[0040] 2. The present invention can ferment a variety of raw materials well by using composite bacteria to ferment the fermentation raw materials during the preparation of microbial fermented feed. By using Bacillus, yeast and lactic acid bacteria, a variety of nutrients in the raw materials can be quickly and efficiently converted, thereby increasing feed utilization and improving feed quality.

[0041] 3. The present invention uses cellulase and protease to hydrolyze the fermentation raw materials when preparing microbial fermentation feed, and first hydrolyzes the macromolecular cellulose and protein in the raw materials into small molecular substances, thereby improving the efficiency of subsequent raw material fermentation, better fermenting and utilizing most of the raw materials, and making the nutrition in the feed more balanced.

[0042] 4. The present invention adds casein and gelatin when preparing the finished product of microbial fermentation feed, so that the feed particles have better adhesion and are not easy to disperse in water, so that they can be better eaten by frogs. In addition, the method of preparing particles by spray drying can obtain feed with less water content, which is easier to preserve. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 The present invention is a flow chart of a method for preparing a frog microbial fermentation feed adopted in an embodiment of the present invention. DETAILED DESCRIPTION

[0044] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] Example 1

[0046] A microbial fermented feed for frogs and a preparation method thereof, such as Figure 1 As shown, the specific steps include:

[0047] S1: Preparation of Jerusalem artichoke powder

[0048] The mud and sand on the surface of the fresh Jerusalem artichoke tuber are washed with clean water, peeled, sliced ​​into 2 mm thick Jerusalem artichoke slices with a slicer, then immersed in a 0.25% citric acid aqueous solution, dried in a blast drying oven, crushed and sieved through a 200-mesh sieve to obtain Jerusalem artichoke whole powder. The Jerusalem artichoke whole powder can be used as a prebiotic and added to feed to regulate the intestinal microecological balance of frogs, promote gastrointestinal absorption and digestion function and improve the immune function. The dissolution rate of the Jerusalem artichoke whole powder is very low, and the frog will not lose too much when eating.

[0049] S2: Activation of Bacillus species

[0050] Use a sterile pipette to inoculate the frozen Bacillus liquid into the solid activation culture medium, put it into the incubator, and culture it at 37°C for 24 hours to obtain a primary activation product. Pick a single colony and inoculate it into the liquid activation culture medium, culture it at 37°C and 200r / min for 44 hours to obtain a secondary activation product, which is stored at 4°C for later use. The activation culture medium in step S2.1 is LB culture medium, and the activation culture medium in step S2.2 is broth culture medium.

[0051] S3: Preparation of composite bacterial suspension

[0052] The bacillus was inoculated into a nutrient agar slant medium, and yeast and lactic acid bacteria were added, and the culture was carried out at 36°C for 22 hours. The composite bacteria were washed with sterile water, and the intermediate bacterial solution I was obtained after heating at 70°C for 30 minutes. The concentration of the intermediate bacterial solution I was adjusted to 110 cfu / mL using a diluent, and the solution was stored at 4°C for later use. The diluent in step S3.2 can be 0.9% sterile sodium chloride solution, 0.1% protein peptide aqueous solution, and sodium chloride-protein peptide buffer with a pH of 7. The composite bacteria are used to ferment the fermentation raw materials, and a variety of raw materials can be fermented well. By using bacillus, yeast and lactic acid bacteria, a variety of nutrients in the raw materials can be quickly and efficiently converted, thereby increasing feed utilization and improving feed quality.

[0053] S4: Enzymatic hydrolysis of fermentation raw materials

[0054] Take 30 parts of soybean meal, 20 parts of corn flour, 3 parts of fish meal and 7 parts of kelp powder by weight, grind them, pass through a 200-mesh sieve to obtain a mixture, add 1.0 part of cellulase, 1.0 part of protease and 100 parts of water to the mixture, control the temperature to 55°C and the pH to 7, treat for 15 hours to obtain a fermentation raw material solution after enzymatic hydrolysis, heat the solution to 100°C for 10 minutes, perform enzyme inactivation treatment to obtain treated fermentation raw materials, first use cellulase and protease to hydrolyze the fermentation raw materials, first hydrolyze the macromolecular cellulose and protein in the raw materials into small molecular substances, improve the efficiency of subsequent raw material fermentation, better ferment and utilize most of the raw materials, and make the nutrition in the feed more balanced.

[0055] S5: Fermentation

[0056] Take out 110 parts of the processed fermentation raw materials by weight, add 30 parts of whole chrysanthemum powder and 2.4 parts of attractant, mix well, further mix the mixture with the composite bacterial suspension, put it into a fermentation machine for fermentation for 23 hours at a temperature of 38°C and a pH of 7 to obtain a fermentation intermediate solution I, and low-temperature dry the fermentation intermediate solution I at a temperature of 33°C and a moisture mass percentage of 32% to obtain a primary fermented feed. The attractant in step S5.1 contains 2 parts of betaine and 12 parts of squid viscera powder.

[0057] S6: Preparation of finished microbial fermented feed

[0058] 3 parts of casein and 5 parts of gelatin are added to 98 parts of primary fermented feed, a small amount of water is added and fully mixed, and then an emulsion passing through a 100-mesh sieve is collected, the emulsion is loaded into a sprayer, and the emulsion is sprayed onto a 5% calcium chloride solution at a pressure of 1500 kPa, and particles are collected, and the particles are placed in a 0.5% calcium chloride solution and heated for 10 minutes at 80°C, and then placed in a carbonic acid solution and soaked for 30 minutes, and then washed with pure water and 50-mesh particles are screened out to obtain a finished microbial fermented feed. By adding casein and gelatin, the feed particles have better adhesion, are not easy to disperse in water, and can be better eaten by frogs, and a method for preparing particles by spray drying can obtain feed with less water content, which is easier to preserve.

[0059] Example 2

[0060] A microbial fermented feed for frogs and a preparation method thereof, such as Figure 1 As shown, the specific steps include:

[0061] S1: Preparation of Jerusalem artichoke powder

[0062] Wash the mud and sand on the epidermis of fresh Jerusalem artichoke tubers with clean water, peel them, cut the Jerusalem artichoke into 1 mm thick Jerusalem artichoke slices with a slicer, and then soak them in a 0.2% citric acid aqueous solution. Dry the Jerusalem artichoke slices in a blast drying oven, grind them and pass them through a 100-mesh sieve to obtain Jerusalem artichoke whole powder. The Jerusalem artichoke whole powder can be used as a prebiotic and added to feed to regulate the intestinal microecological balance of frogs, promote gastrointestinal absorption and digestion functions, and improve the immune function. The dissolution rate of the Jerusalem artichoke whole powder is very low, and the frogs will not lose too much when eating.

[0063] S2: Activation of Bacillus species

[0064] Use a sterile pipette to inoculate the frozen Bacillus liquid into the solid activation culture medium, put it into the incubator, and culture it at 36°C for 26 hours to obtain a primary activation product. Pick a single colony and inoculate it into the liquid activation culture medium, culture it at 36°C and 220r / min for 45 hours to obtain a secondary activation product, which is stored at 3°C ​​for later use. The activation culture medium in step S2.1 is LB culture medium, and the activation culture medium in step S2.2 is broth culture medium.

[0065] S3: Preparation of composite bacterial suspension

[0066] The bacillus was inoculated into a nutrient agar slant medium, and yeast and lactic acid bacteria were added, and the culture was carried out at 35°C for 24 hours. The composite bacteria were washed with sterile water, and the intermediate bacterial solution I was obtained after heating at 60°C for 20 minutes. The concentration of the intermediate bacterial solution I was adjusted to 120 cfu / mL using a diluent, and the solution was stored at 3°C ​​for later use. The diluent in step S3.2 can be 0.8% sterile sodium chloride solution, 0.12% protein peptide aqueous solution, and sodium chloride-protein peptide buffer with a pH of 8. The composite bacteria are used to ferment the fermentation raw materials, and a variety of raw materials can be fermented well. By using bacillus, yeast and lactic acid bacteria, a variety of nutrients in the raw materials can be quickly and efficiently converted, thereby increasing feed utilization and improving feed quality.

[0067] S4: Enzymatic hydrolysis of fermentation raw materials

[0068] Take 30 parts of soybean meal, 20 parts of corn flour, 3 parts of fish meal and 7 parts of kelp powder by weight, grind them, pass through a 220-mesh sieve to obtain a mixture, add 1.0 part of cellulase, 1.0 part of protease and 110 parts of water to the mixture, control the temperature to 50°C and the pH to 6, treat for 14 hours to obtain a fermentation raw material solution after enzymatic hydrolysis, heat the solution to 110°C for 12 minutes, perform enzyme inactivation treatment to obtain treated fermentation raw materials, first use cellulase and protease to hydrolyze the fermentation raw materials, first hydrolyze the macromolecular cellulose and protein in the raw materials into small molecular substances, improve the efficiency of subsequent raw material fermentation, better ferment and utilize most of the raw materials, and make the nutrition in the feed more balanced.

[0069] S5: Fermentation

[0070] Take out 110 parts of the processed fermentation raw materials by weight, add 30 parts of chrysanthemum whole powder and 2.4 parts of attractant, mix well, further mix the mixture with the composite bacterial suspension, put it into a fermentation machine for fermentation for 24 hours at a temperature of 36°C and a pH of 6 to obtain a fermentation intermediate solution I, and dry the fermentation intermediate solution I at a temperature of 34°C and a moisture content of 30% to obtain a primary fermented feed. The attractant in step S5.1 contains 2 parts of betaine and 12 parts of squid viscera powder.

[0071] S6: Preparation of finished microbial fermented feed

[0072] 3 parts of casein and 5 parts of gelatin are added to 98 parts of primary fermented feed, a small amount of water is added and mixed thoroughly, an emulsion passing through a 110-mesh sieve is collected, the emulsion is loaded into a sprayer, and the emulsion is sprayed onto a 4% calcium chloride solution at a pressure of 1400 kPa, particles are collected, the particles are placed in a 0.4% calcium chloride solution and heated for 12 minutes at 70°C, and then placed in a carbonic acid solution and soaked for 20 minutes, and 40-mesh particles are screened out after washing with pure water to obtain a finished microbial fermented feed. By adding casein and gelatin, the feed particles have better adhesion, are not easy to disperse in water, and can be better eaten by frogs. In addition, a method for preparing particles by spray drying can obtain feed with less water content, which is easier to preserve.

[0073] Example 3

[0074] A microbial fermented feed for frogs and a preparation method thereof, such as Figure 1 As shown, the specific steps include:

[0075] S1: Preparation of Jerusalem artichoke powder

[0076] The mud and sand on the surface of the fresh Jerusalem artichoke tuber are washed with clean water, peeled, sliced ​​into 2 mm thick Jerusalem artichoke slices with a slicer, then immersed in a 0.25% citric acid aqueous solution, dried in a blast drying oven, crushed and sieved through a 200-mesh sieve to obtain Jerusalem artichoke whole powder. The Jerusalem artichoke whole powder can be used as a prebiotic and added to feed to regulate the intestinal microecological balance of frogs, promote gastrointestinal absorption and digestion function and improve the immune function. The dissolution rate of the Jerusalem artichoke whole powder is very low, and the frog will not lose too much when eating.

[0077] S2: Activation of Bacillus species

[0078] Use a sterile pipette to inoculate the frozen Bacillus liquid into the solid activation culture medium, put it into the incubator, and culture it at 37°C for 24 hours to obtain a primary activation product. Pick a single colony and inoculate it into the liquid activation culture medium, culture it at 37°C and 200r / min for 44 hours to obtain a secondary activation product, which is stored at 4°C for later use. The activation culture medium in step S2.1 is LB culture medium, and the activation culture medium in step S2.2 is broth culture medium.

[0079] S3: Preparation of composite bacterial suspension

[0080] The bacillus was inoculated into a nutrient agar slant medium, and yeast and lactic acid bacteria were added, and the culture was carried out at 36°C for 22 hours. The composite bacteria were washed with sterile water, and the intermediate bacterial solution I was obtained after heating at 70°C for 30 minutes. The concentration of the intermediate bacterial solution I was adjusted to 110 cfu / mL using a diluent, and the solution was stored at 4°C for later use. The diluent in step S3.2 can be 0.9% sterile sodium chloride solution, 0.1% protein peptide aqueous solution, and sodium chloride-protein peptide buffer with a pH of 7. The composite bacteria are used to ferment the fermentation raw materials, and a variety of raw materials can be fermented well. By using bacillus, yeast and lactic acid bacteria, a variety of nutrients in the raw materials can be quickly and efficiently converted, thereby increasing feed utilization and improving feed quality.

[0081] S4: Enzymatic hydrolysis of fermentation raw materials

[0082] 40 parts of soybean meal, 25 parts of corn flour, 5 parts of fish meal and 10 parts of kelp powder are taken by weight, crushed, and passed through a 200-mesh sieve to obtain a mixture, 1.5 parts of cellulase, 1.2 parts of protease, and 100 parts of water are added to the mixture, the temperature is controlled to be 55°C and the pH is 7, and the mixture is treated for 15 hours to obtain a fermentation raw material solution after enzymatic hydrolysis, the solution is heated to 100°C for 10 minutes, and enzyme inactivation treatment is performed to obtain treated fermentation raw materials, cellulase and protease are first used to hydrolyze the fermentation raw materials, and macromolecular cellulose and protein in the raw materials are first hydrolyzed into small molecular substances, so as to improve the efficiency of subsequent raw material fermentation, better ferment and utilize most of the raw materials, and make the nutrition in the feed more balanced.

[0083] S5: Fermentation

[0084] Take out 120 parts of the processed fermentation raw materials by weight, add 40 parts of chrysanthemum whole powder and 3.6 parts of attractant, mix well, further mix the mixture with the composite bacterial suspension, put it into a fermentation machine for fermentation for 23 hours at a temperature of 38°C and a pH of 7 to obtain a fermentation intermediate solution I, and low-temperature dry the fermentation intermediate solution I at a temperature of 33°C and a moisture mass percentage of 32% to obtain a primary fermented feed. The attractant in step S5.1 contains 3 parts of betaine and 15 parts of squid viscera powder.

[0085] S6: Preparation of finished microbial fermented feed

[0086] Four parts of casein and six parts of gelatin are added to 100 parts of primary fermented feed, a small amount of water is added and fully mixed, an emulsion passing through a 100-mesh sieve is collected, the emulsion is loaded into a sprayer, and the emulsion is sprayed onto a 5% calcium chloride solution at a pressure of 1500 kPa, particles are collected, the particles are placed in a 0.5% calcium chloride solution and heated at 80°C for 10 minutes, and then placed in a carbonic acid solution and soaked for 30 minutes, and particles of 50 mesh are screened out after washing with pure water to obtain a finished microbial fermented feed. By adding casein and gelatin, the feed particles have better adhesion, are not easy to disperse in water, and can be better eaten by frogs. In addition, a method for preparing particles by spray drying can obtain feed with less water content, which is easier to preserve.

[0087] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A microbial fermented feed suitable for frogs and a preparation method thereof, characterized in that: The specific steps include: S1: Preparation of Jerusalem artichoke powder Wash the mud and sand on the epidermis of fresh Jerusalem artichoke tubers with clean water, peel them, slice the Jerusalem artichokes into Jerusalem artichoke slices with a thickness of 1-3 mm using a slicer, then soak them in a 0.2-0.3% citric acid aqueous solution, dry the Jerusalem artichoke slices in an air drying oven, crush them, and pass them through a 100-300 mesh sieve to obtain Jerusalem artichoke whole powder; S2: Activation of Bacillus species Use a sterile pipette to inoculate the frozen Bacillus solution into the solid activated culture medium and place it in the incubator. Culture at 36-38℃ for 22-26h, pick a single colony and inoculate it into liquid activation medium, culture at 36-38℃ and 180-220r / min for 43-45h, and store at 3-5℃ for later use; S3: Preparation of composite bacterial suspension Inoculate the activated Bacillus into the nutrient agar slant medium, add yeast and lactic acid bacteria solution, culture at 35-37℃ for 20-24h, wash off the composite bacteria with sterile water, and heat at 60-80℃. After 20-40 minutes, the intermediate bacterial solution I is obtained, and the concentration of the intermediate bacterial solution I is adjusted to 100-120 cfu / mL, store at 3-5℃ for future use; S4: Enzymatic hydrolysis of fermentation raw materials Take 15-40 parts of soybean meal, 3-25 parts of corn flour, 1-5 parts of fish meal and 3-10 parts of kelp powder by weight, grind, pass through a 180-220 mesh sieve, add 0.5-1.5 parts of cellulase, 0.8-1.2 parts of protease, and 90-110 parts of water, control the temperature to 50-60° C., the pH to 6-8, treat for 14-16 hours, then heat to 90-110° C., for 8-12 minutes, perform enzyme inactivation treatment, and obtain a treated fermentation raw material; S5: Fermentation 100-120 parts by weight of the processed fermentation raw materials are taken out, 20-40 parts of Jerusalem artichoke powder and 1.8-3.6 parts of attractants are added, and the mixture is evenly mixed, and the mixture is further mixed with the composite bacterial suspension, and put into a fermentation machine for fermentation for 22-24 hours at a temperature of 36-40° C. and a pH of 6-8, and low-temperature drying is performed at a temperature of 32-34° C. and a moisture mass percentage of 30%-35%, to obtain a primary fermented feed; S6: Preparation of finished microbial fermented feed Casein and gelatin are added to the primary fermented feed, and after adding a small amount of water to fully mix, the emulsion passing through a 90-110 mesh sieve is added into a sprayer, and it is sprayed into a 4-6% calcium chloride solution under pressure, and the particles are collected, and then placed in a 0.4-0.6% calcium chloride solution and heated for 8-12 minutes at 70-90°C, and then placed in a carbonate solution and soaked for 20-40 minutes, and then washed with pure water and screened to obtain a finished microbial fermented feed.

2. A microbial fermented feed suitable for frogs and a preparation method thereof according to claim 1, characterized in that: Step S2 activates the Bacillus species, specifically comprising the following steps: S2.1: Use a sterile pipette to inoculate the frozen Bacillus solution into the solid activation medium, place it in an incubator, and culture it at a constant temperature of 36-38°C for 22-26 hours to obtain a primary activation product; S2.2: Pick a single colony and inoculate it into liquid activation culture medium, culture it at 36-38℃ and 180-220r / min for 43-45h to obtain secondary activation product, and store it at 3-5℃ for later use.

3. A microbial fermented feed suitable for frogs and a preparation method thereof according to claim 2, characterized in that: The activation medium in step S2.1 is LB medium, and the activation medium in step S2.2 is broth medium.

4. A microbial fermented feed suitable for frogs and a preparation method thereof according to claim 1, characterized in that: The preparation of S3 composite bacterial suspension specifically includes the following steps: S3.1: Inoculate the Bacillus into the nutrient agar slant medium, add yeast and lactic acid bacteria, culture at 35-37℃ for 20-24h, wash the composite bacteria with sterile water, and heat at 60-80℃ for 20-40min to obtain intermediate bacterial solution I; S3.2: Use diluent to adjust the concentration of intermediate bacterial solution I to 100-120 cfu / mL and store at 3-5℃ for later use.

5. A microbial fermented feed suitable for frogs and a preparation method thereof according to claim 4, characterized in that: The diluent in step S3.2 can be 0.8-1.0% sterile sodium chloride solution, 0.08-0.12% protein peptide aqueous solution and sodium chloride-protein peptide buffer with a pH of 6-8.

6. A microbial fermented feed suitable for frogs and a preparation method thereof according to claim 1, characterized in that: Step S4 is to perform enzymatic hydrolysis on the fermentation raw material, which specifically includes the following steps: S4.1: Take 15-40 parts by weight of soybean meal, 3-25 parts of corn meal, 1-5 parts of fish meal and 3-10 parts of kelp meal, grind them, and pass them through a 180-220 mesh sieve to obtain a mixture; S4.2: adding 0.5-1.5 parts of cellulase, 0.8-1.2 parts of protease, and 90-110 parts of water to the mixture, controlling the temperature to 50-60°C and the pH to 6-8, treating for 14-16 hours, and obtaining a fermentation raw material solution after enzymatic hydrolysis; S4.3: The solution is heated to 90-110°C for 8-12 minutes to inactivate the enzyme and obtain the treated fermentation raw material.

7. A microbial fermented feed suitable for frogs and a preparation method thereof according to claim 1, characterized in that: Step S5 is to carry out fermentation, which specifically comprises the following steps: S5.1: Take out 100-120 parts by weight of the processed fermented raw materials, add 20-40 parts of chrysanthemum powder and 1.8-3.6 parts of attractant, and mix well; S5.2: the mixture is further mixed with the composite bacterial suspension, and placed in a fermentation machine for fermentation for 22-24 hours at a temperature of 36-40°C and a pH of 6-8 to obtain a fermentation intermediate solution I; S5.3: The fermentation intermediate solution I is dried at low temperature at a temperature of 32-34° C. and a water content of 30%-35% by mass to obtain primary fermented feed.

8. The microbial fermented feed suitable for frogs and the preparation method thereof according to claim 6, characterized in that: The attractant in step S5.1 comprises 1-3 parts of betaine and 8-15 parts of squid viscera powder.

9. The microbial fermented feed suitable for frogs and the preparation method thereof according to claim 1, characterized in that: Step S6 is to prepare a finished product of microbial fermentation feed, which specifically comprises the following steps: S6.1: Add 2-4 parts of casein and 4-6 parts of gelatin to 96-100 parts of primary fermented feed, add a small amount of water and mix thoroughly, and collect the emulsion that passes through a 90-110 mesh sieve; S6.2: Load the emulsion into a sprayer, spray it onto a 4-6% calcium chloride solution at a pressure of 1400-1600 kPa, and collect the particles; S6.3: Place the particles in a 0.4-0.6% calcium chloride solution and heat for 8-12 minutes at 70-90°C, then soak them in a carbonate solution for 20-40 minutes, wash them with pure water and select particles of 40-60 mesh to obtain the finished microbial fermented feed.

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