Anti-aging composition containing ergothioneine, glutathione and fish collagen

Abstract

The invention discloses an anti-aging composition containing ergothioneine, glutathione and fish collagen, relates to the technical field of anti-aging compositions, and aims to solve the technical problems that a traditional anti-aging composition lacks attention to the aspects of cell energy metabolism, barrier repair and the like, and the overall skin improvement effect is not obvious. The ergothioneine is used as a natural antioxidant, and the content of the ergothioneine is 0.01-5wt%; the ergothioneine participates in a synergistic effect of an intracellular antioxidant enzyme system, so that tyrosinase activity is effectively inhibited, melanogenesis is reduced, and DNA and protein are protected from oxidation damage; in a simulated strong ultraviolet environment, compared with skin cells not using the ergothioneine-containing product, the content of free radicals in the cells is reduced by 30% after the ergothioneine-containing product is used; the ergothioneine can also regulate a cell signal channel, promote cell metabolism and maintain normal growth of cells. The mask has the advantages of accelerating skin metabolism and improving the self-repairing ability of the skin.

Description

Technical Field

[0001] The present invention relates to the technical field of anti-aging compositions, and more specifically to an anti-aging composition containing ergothioneine, glutathione and fish collagen. Background Art

[0002] As people's living standards improve and their pursuit of beauty continues to increase, the demand for anti-aging products in the skin care field is growing. Skin aging is mainly caused by the combined effects of multiple internal and external factors;

[0003] From the perspective of external factors, ultraviolet radiation is one of the key factors leading to skin aging. Long-term exposure to ultraviolet rays will cause the skin to produce a large number of free radicals. Free radicals are highly oxidizing and will attack biological macromolecules such as DNA and proteins in skin cells, destroying cell structure and function, causing cell damage, and then leading to skin aging, wrinkles, pigmentation and other problems. At the same time, harmful substances in environmental pollution such as particulate matter and chemical pollutants will also adhere to the surface of the skin and enter the skin through penetration, triggering oxidative stress reactions and accelerating the process of skin aging.

[0004] From the internal factors, as we age, our body's metabolism slows down, the cell's renewal capacity declines, the functions of organelles such as mitochondria in skin cells decline, energy synthesis decreases, and cell vitality decreases. The synthesis of collagen and elastic fibers in the skin gradually decreases and the decomposition speeds up, causing the skin to lose elasticity and sag. At the same time, the activity of the body's antioxidant enzyme system decreases, the free radical scavenging ability decreases, and free radicals accumulate in the body, further aggravating the damage to the skin.

[0005] In the prior art, although there are many anti-aging skin care products, they often have certain limitations. Some products contain only a single antioxidant ingredient, and the antioxidant effect is not comprehensive and lasting enough, and cannot effectively cope with the attack of multiple free radicals. Although some products can supplement collagen, due to the lack of attention to aspects such as cell energy metabolism and barrier repair, the overall improvement effect of the skin is not obvious. Moreover, the active ingredients of many products have poor stability and are prone to lose activity during production, storage and use, reducing product efficacy. In addition, for people with sensitive skin, most anti-aging products cannot meet their skin care needs due to their strong irritation and are prone to cause adverse reactions such as allergies. In view of this, we propose an anti-aging composition containing ergothioneine, glutathione, and fish collagen. Summary of the invention

[0006] The purpose of the present invention is to provide an anti-aging composition containing ergothioneine, glutathione and fish collagen to solve the technical problem that traditional anti-aging compositions lack attention to aspects such as cell energy metabolism and barrier repair, and the overall improvement effect of the skin is not obvious.

[0007] In order to solve the above technical problems, the present invention provides the following technical solution: an anti-aging composition containing ergothioneine, glutathione and fish collagen, comprising:

[0008] Ergothioneine:

[0009] Ergothioneine is used as a natural antioxidant, and its content is 0.01-5wt%;

[0010] Ergothioneine effectively inhibits tyrosinase activity, reduces melanin production, and protects DNA and proteins from oxidative damage by participating in the synergistic effect of the intracellular antioxidant enzyme system.

[0011] In a simulated strong ultraviolet environment, the free radical content in the cells was reduced by 30% compared to skin cells that did not use ergothioneine products;

[0012] Ergothioneine can also regulate cell signaling pathways, promote cell metabolism, maintain normal cell growth, reduce the release of inflammatory factors, play an anti-inflammatory role, provide auxiliary effects for the prevention of cardiovascular diseases, slow down skin aging and prevent ultraviolet damage, promote epidermal cell renewal, and accelerate the shedding of aging keratinocytes by 15%, keeping the skin young.

[0013] Glutathione:

[0014] Glutathione is a small molecular active oligopeptide, with a content of 0.001-3wt%;

[0015] With the help of GSH-peroxidase-reductase system, it can accurately capture and remove harmful free radicals and lipid peroxides in the body;

[0016] In skin tissue, it regulates the activity of enzymes related to melanin synthesis, prevents skin aging and pigment accumulation, and makes the skin appear naturally shiny;

[0017] Glutathione can enhance the skin's barrier function and reduce water loss through the epidermis by 18%;

[0018] By interacting with the water channel proteins in skin cells, it regulates the transport and retention of water, stabilizes the water balance inside the skin, improves the skin's moisturizing ability, and fundamentally improves the dry and dehydrated state of the skin;

[0019] Collagen:

[0020] Collagen is used as a high-quality collagen source, with a content of 0.1 to 10 wt%;

[0021] Rich in various amino acids, it stimulates fibroblasts to secrete more collagen and elastic fibers;

[0022] During the skin damage repair process, it attracts immune cells to gather and accelerate wound healing. For minor damaged skin, the skin repair rate can reach 70% within 3-5 days.

[0023] Collagen can interact with integrins in the skin, enhance cell adhesion, improve skin elasticity and firmness, improve skin sagging, and make the skin feel firmer and smoother;

[0024] PQQ-Pyrroloquinoline quinone disodium salt:

[0025] PQQ-pyrroloquinoline quinone disodium salt has a unique function of activating mitochondria, and the content is 0.005-2wt%;

[0026] PQQ-pyrroloquinoline quinone disodium salt can increase the number of mitochondria and improve their activity, providing sufficient power for cellular energy metabolism;

[0027] In the process of energy metabolism, it promotes the synthesis of adenosine triphosphate (ATP) in cells, thereby enhancing cell vitality;

[0028] After the treated cells were cultured in a medium containing PQQ-pyrroloquinoline quinone disodium salt, the cell energy metabolism level increased by 40%, accelerating skin metabolism, delaying the skin aging process, increasing the proliferation rate of skin cells by 1.5 times, and reducing the accumulation of aging cells;

[0029] It can also regulate the redox balance within cells, enhance cells' resistance to environmental stress, and protect cells from external damage.

[0030] Preferably, it also includes:

[0031] Coenzyme Q10: content 0.01-2wt%;

[0032] Coenzyme Q10 has an antioxidant effect, which can help remove free radicals, protect the integrity of cell membranes, enhance the skin's antioxidant capacity, help restore skin elasticity, and reduce wrinkles;

[0033] In skin cell experiments, a combination with added coenzyme Q10 can slow down cell aging by 20%;

[0034] Coenzyme Q10 can promote cell respiration, improve cell oxygen utilization, enhance cell energy production, and provide continuous power support for the skin;

[0035] At the same time, it can also work synergistically with other antioxidant ingredients to form a powerful antioxidant network and enhance the overall antioxidant effect;

[0036] Astaxanthin: content 0.001~1wt%;

[0037] Astaxanthin is a highly effective antioxidant, with an antioxidant capacity 550 times that of vitamin E. It can effectively quench singlet oxygen, reduce free radical damage to the skin, improve skin dullness, and enhance skin brightness and color. After using a composition containing astaxanthin for 2 months, skin brightness can be increased by 30%. Astaxanthin has a unique molecular structure that enables it to penetrate the skin's multiple barriers and penetrate deep into the cells to exert an antioxidant effect. In addition, it can inhibit the activity of key enzymes in the production process of melanin, reduce the production of melanin, and improve the problem of skin dullness from the root.

[0038] Ceramide: content of 0.05-3wt%;

[0039] Ceramide can repair damaged skin barrier, enhance the skin's self-protection ability, reduce water loss, and maintain the skin's hydration. The composition containing ceramide can reduce the skin's water loss rate by 22%. Ceramide can interact with lipids in the skin's stratum corneum to form a tight lipid bilayer structure, restoring the integrity of the skin barrier. At the same time, it can also regulate the skin's immune response, enhance the skin's resistance, and reduce the irritation of the skin by external harmful substances.

[0040] Preferably, it also includes plant extracts: green tea extract, chamomile extract, grape seed extract;

[0041] Green tea extract, content of 0.05-2wt%;

[0042] Rich in antioxidant ingredients such as tea polyphenols, it can remove free radicals, inhibit lipid peroxidation, reduce skin inflammation, and has antibacterial, anti-inflammatory and skin-soothing effects. In the inflammatory skin model experiment, the use of a composition containing green tea extract can reduce inflammatory factors by 40%. The tea polyphenols in green tea extract can inhibit the release of inflammatory mediators, regulate the expression of inflammation-related genes, and reduce skin inflammation at the molecular level. In addition, it can promote the proliferation and differentiation of skin cells and accelerate the skin's self-repair process.

[0043] Chamomile extract, content of 0.03-1.5wt%;

[0044] It has the effects of soothing the skin, anti-allergic and repairing, can reduce skin sensitivity symptoms and enhance skin tolerance. For people with sensitive skin, the use of a composition containing chamomile extract can effectively reduce skin sensitivity by 45%. The flavonoids in chamomile extract have antioxidant and anti-inflammatory effects, which can reduce skin allergy symptoms such as redness, swelling and itching. At the same time, it can also regulate the skin's nerve conduction, relieve the skin's tension and make the skin more comfortable.

[0045] Grape seed extract, content of 0.02-1wt%;

[0046] It is rich in proanthocyanidins, has strong antioxidant capacity, can protect collagen from free radical damage, promote collagen synthesis, improve skin firmness and elasticity. After using a composition containing grape seed extract, the elasticity of the skin is increased by 28%. The proanthocyanidins in grape seed extract can activate the signal pathway related to collagen synthesis and promote fibroblasts to secrete collagen. In addition, it can also inhibit the activity of matrix metalloproteinases, reduce collagen degradation, and maintain the collagen content in the skin.

[0047] Preferably, ergothioneine, glutathione, fish collagen, PQQ-pyrroloquinoline quinone disodium salt, coenzyme Q10, astaxanthin, ceramide and plant extracts work synergistically to enhance the anti-aging effect from multiple aspects such as anti-oxidation, repairing the skin barrier, promoting cell metabolism, stimulating collagen synthesis, etc., and significantly improve the skin condition;

[0048] Different ingredients work together to form a comprehensive anti-aging system;

[0049] Antioxidant ingredients can remove free radicals, reduce damage to cells, and create a good environment for normal cell metabolism; ingredients that repair skin barrier can enhance the skin's self-protection ability, prevent water loss and the invasion of external harmful substances; ingredients that promote cell metabolism can accelerate cell renewal and improve skin vitality; ingredients that stimulate collagen synthesis can increase skin elasticity and firmness and reduce the formation of wrinkles.

[0050] Preferably, it also includes safe and non-irritating auxiliary materials, which include: moisturizers, emulsifiers, preservatives;

[0051] The moisturizer is glycerin, with a content of 2-10wt%, which is used to maintain the moisture content of the composition, enhance the moisturizing effect of the product, and keep the skin hydrated. Glycerin can absorb moisture in the air and provide continuous moisturizing effect for the skin.

[0052] The emulsifier is polysorbate-20, with a content of 0.5-3wt%, which is used to ensure that the ingredients are evenly dispersed to form a stable emulsion system, improve the stability and texture of the product. The emulsifier can make the oil phase and the water phase blend with each other to form a uniform emulsion, thereby improving the stability and feel of the product.

[0053] The preservative is potassium sorbate, with a content of 0.01-0.5wt%. On the premise of ensuring product safety, it prevents the growth of microorganisms and extends the shelf life of the product. The preservative can inhibit the growth of microorganisms such as bacteria and molds, ensuring the safety of the product during storage and use.

[0054] Preferably, the composition can be used alone or with common auxiliary materials to prepare skin care product formulations such as creams, lotions, essences, moisturizers, and facial masks to meet different skin types and skin care needs:

[0055] Cream dosage form: By adding appropriate amounts of oil and wax ingredients, it has good occlusive properties and can provide deep nourishment to the skin. It is suitable for dry and mature skin to use at night. The oil in the cream can form a protective film on the skin surface to prevent moisture loss, while slowly releasing active ingredients into the skin to exert a long-lasting nourishing effect;

[0056] Emulsion dosage form: The texture is refreshing. By adjusting the emulsification system and oil content, it is easy to absorb and suitable for daily care of neutral and oily skin. The texture of the emulsion is light and can quickly penetrate into the skin to provide moisture and nutrition to the skin without causing greasy feeling.

[0057] Essence: Rich in high-concentration active ingredients, it uses small molecule penetration technology to accurately penetrate the bottom layer of the skin and play an efficient repair role. The active ingredients in the essence have been specially treated to quickly penetrate the skin barrier and reach the bottom layer of the skin to accurately repair skin problems;

[0058] Moisturizing water: With water as the base, adding appropriate amount of moisturizer and active ingredients, it can replenish moisture for the skin at any time and adjust the pH value of the skin. Moisturizing water can quickly replenish moisture for the skin, keep the skin moist, and at the same time adjust the acid-base balance of the skin and enhance the skin's resistance;

[0059] Mask liquid: With the help of the mask carrier, intensive nutrition delivery can be achieved in a short period of time, allowing the active ingredients to quickly penetrate the skin. The active ingredients in the mask liquid can quickly penetrate into the skin under the sealing effect of the mask, providing intensive nutrition care for the skin.

[0060] Preferably, after safety and efficacy tests, the irritation index for people with sensitive skin is lower than 0.2. After 3 months of continuous use, skin wrinkles can be reduced by 35%, skin tone can be brightened by 2 degrees, and elasticity can be increased by 32%. It has excellent stability and safety, and can maintain the stability and effectiveness of active ingredients for a long time. After storage for 18 months at 4°C-40°C, humidity 30%-70% and light conditions, the loss rate of active ingredients is less than 8%.

[0061] Preferably, ergothioneine, glutathione, fish collagen, PQQ-pyrroloquinoline quinone disodium salt, coenzyme Q10, astaxanthin, ceramide, plant extract and other active ingredients are uniformly mixed in proportion, and safe and non-irritating excipients or carriers are added to obtain the product through the following steps:

[0062] The emulsification step adopts advanced micro-emulsification technology and selects environmentally friendly, mild and efficient emulsifiers to ensure that the ingredients are evenly dispersed and form a stable micro-emulsion system, thereby improving the penetration efficiency of active ingredients. Micro-emulsification technology can make the emulsion particle size smaller and the distribution more even, thereby improving the penetration ability of active ingredients and making the product more effective.

[0063] The homogenization step uses high-pressure homogenization equipment to refine the particles to below the nanometer level, thereby improving the fineness and absorbency of the product. High-pressure homogenization equipment can refine the raw material particles, making them easier to be absorbed by the skin and improving the product's use effect.

[0064] The filling process is carried out in a sterile purification workshop, strictly controlling the microbial content to ensure product quality and safety. In the entire preparation process, environmental parameters such as temperature and humidity are strictly controlled to ensure that the activity of each ingredient is not affected. Sterile purification workshops and strict environmental control can ensure the quality and safety of the product and the activity and efficacy of each ingredient.

[0065] Compared with the prior art, the present invention has the following beneficial effects:

[0066] 1. From the perspective of anti-oxidation, the present invention uses a variety of strong antioxidant ingredients to work synergistically to comprehensively remove free radicals. Ergothioneine, glutathione, coenzyme Q10, astaxanthin, etc. jointly build an antioxidant defense line to reduce the damage of free radicals to cells, effectively reduce the oxidative damage caused by ultraviolet rays and environmental factors, and delay the aging process of the skin. In terms of skin repair and regeneration, fish collagen stimulates fibroblasts to secrete collagen and elastic fibers, and combines PQQ-pyrroloquinoline quinone disodium salt to promote cell energy metabolism and proliferation, accelerate skin metabolism, and improve skin self-repair ability. The repair rate of slightly damaged skin reaches 70% within 3-5 days, and the proliferation rate of skin cells is increased by 1.5 times. Long-term use can reduce wrinkles by 35% and improve skin elasticity by 32%. At the same time, plant extracts promote cell proliferation and differentiation, further assist skin repair, green tea extract can reduce inflammatory factors by 40%, chamomile extract reduces the sensitivity of sensitive skin by 45%, and grape seed extract increases skin elasticity by 28%, solving the problem that the traditional anti-aging composition lacks attention to aspects such as cell energy metabolism and barrier repair, and the overall improvement effect of the skin is not obvious.

[0067] 2. The present invention is suitable for all skin types, especially sensitive skin. The mild ingredients in the formula, such as chamomile extract, can soothe the skin and reduce the irritation index to below 0.2. Through scientific proportions and processes, the loss rate of active ingredients is less than 8% after storage for 18 months under 4°C-40°C, humidity 30%-70% and light conditions, ensuring the long-term effectiveness of the product, and can be made into a variety of dosage forms to meet different needs, such as creams suitable for nighttime nourishment of dry mature skin, lotions suitable for daily maintenance of neutral oily skin, essences for precise repair, moisturizers for hydration and pH adjustment at any time, and facial masks for intensive nutrient delivery. DETAILED DESCRIPTION

[0068] In order to facilitate those skilled in the art to understand the technical solution of the present invention, the technical solution of the present invention is further explained in conjunction with the specification.

[0069] An anti-aging composition containing ergothioneine, glutathione and fish collagen, characterized in that:

[0070] include:

[0071] Ergothioneine:

[0072] Ergothioneine is used as a natural antioxidant, and its content is 0.01-5wt%;

[0073] Ergothioneine effectively inhibits tyrosinase activity, reduces melanin production, and protects DNA and proteins from oxidative damage by participating in the synergistic effect of the intracellular antioxidant enzyme system.

[0074] In a simulated strong ultraviolet environment, the free radical content in the cells was reduced by 30% compared to skin cells that did not use ergothioneine products;

[0075] Ergothioneine can also regulate cell signaling pathways, promote cell metabolism, maintain normal cell growth, reduce the release of inflammatory factors, play an anti-inflammatory role, provide auxiliary effects for the prevention of cardiovascular diseases, slow down skin aging and prevent ultraviolet damage, promote epidermal cell renewal, and accelerate the shedding of aging keratinocytes by 15%, keeping the skin young.

[0076] Glutathione:

[0077] Glutathione is a small molecular active oligopeptide, with a content of 0.001-3wt%;

[0078] With the help of GSH-peroxidase-reductase system, it can accurately capture and remove harmful free radicals and lipid peroxides in the body;

[0079] In skin tissue, it regulates the activity of enzymes related to melanin synthesis, prevents skin aging and pigment accumulation, and makes the skin appear naturally shiny;

[0080] Glutathione can enhance the skin's barrier function and reduce water loss through the epidermis by 18%;

[0081] By interacting with the water channel proteins in skin cells, it regulates the transport and retention of water, stabilizes the water balance inside the skin, improves the skin's moisturizing ability, and fundamentally improves the dry and dehydrated state of the skin;

[0082] Collagen:

[0083] Collagen is used as a high-quality collagen source, with a content of 0.1 to 10 wt%;

[0084] Rich in various amino acids, it stimulates fibroblasts to secrete more collagen and elastic fibers;

[0085] During the skin damage repair process, it attracts immune cells to gather and accelerate wound healing. For minor damaged skin, the skin repair rate can reach 70% within 3-5 days.

[0086] Collagen can interact with integrins in the skin, enhance cell adhesion, improve skin elasticity and firmness, improve skin sagging, and make the skin feel firmer and smoother;

[0087] PQQ-Pyrroloquinoline quinone disodium salt:

[0088] PQQ-pyrroloquinoline quinone disodium salt has a unique function of activating mitochondria, and the content is 0.005-2wt%;

[0089] PQQ-pyrroloquinoline quinone disodium salt can increase the number of mitochondria and improve their activity, providing sufficient power for cellular energy metabolism;

[0090] In the process of energy metabolism, it promotes the synthesis of adenosine triphosphate (ATP) in cells, thereby enhancing cell vitality;

[0091] After the treated cells were cultured in a medium containing PQQ-pyrroloquinoline quinone disodium salt, the cell energy metabolism level increased by 40%, accelerating skin metabolism, delaying the skin aging process, increasing the proliferation rate of skin cells by 1.5 times, and reducing the accumulation of aging cells;

[0092] It can also regulate the redox balance within cells, enhance cells' resistance to environmental stress, and protect cells from external damage.

[0093] In an embodiment of the present invention, it also includes:

[0094] Coenzyme Q10: content 0.01-2wt%;

[0095] Coenzyme Q10 has an antioxidant effect, which can help remove free radicals, protect the integrity of cell membranes, enhance the skin's antioxidant capacity, help restore skin elasticity, and reduce wrinkles;

[0096] In skin cell experiments, a combination with added coenzyme Q10 can slow down cell aging by 20%;

[0097] Coenzyme Q10 can promote cell respiration, improve cell oxygen utilization, enhance cell energy production, and provide continuous power support for the skin;

[0098] At the same time, it can also work synergistically with other antioxidant ingredients to form a powerful antioxidant network and enhance the overall antioxidant effect;

[0099] Astaxanthin: content 0.001~1wt%;

[0100] Astaxanthin is a highly effective antioxidant, with an antioxidant capacity 550 times that of vitamin E. It can effectively quench singlet oxygen, reduce free radical damage to the skin, improve skin dullness, and enhance skin brightness and color. After using a composition containing astaxanthin for 2 months, skin brightness can be increased by 30%. Astaxanthin has a unique molecular structure that enables it to penetrate the skin's multiple barriers and penetrate deep into the cells to exert an antioxidant effect. In addition, it can inhibit the activity of key enzymes in the production process of melanin, reduce the production of melanin, and improve the problem of skin dullness from the root.

[0101] Ceramide: content of 0.05-3wt%;

[0102] Ceramide can repair damaged skin barrier, enhance the skin's self-protection ability, reduce water loss, and maintain the skin's hydration. The composition containing ceramide can reduce the skin's water loss rate by 22%. Ceramide can interact with lipids in the skin's stratum corneum to form a tight lipid bilayer structure, restoring the integrity of the skin barrier. At the same time, it can also regulate the skin's immune response, enhance the skin's resistance, and reduce the irritation of the skin by external harmful substances.

[0103] In an embodiment of the present invention, plant extracts are also included: green tea extract, chamomile extract, grape seed extract;

[0104] Green tea extract, content of 0.05-2wt%;

[0105] Rich in antioxidant ingredients such as tea polyphenols, it can remove free radicals, inhibit lipid peroxidation, reduce skin inflammation, and has antibacterial, anti-inflammatory and skin-soothing effects. In the inflammatory skin model experiment, the use of a composition containing green tea extract can reduce inflammatory factors by 40%. The tea polyphenols in green tea extract can inhibit the release of inflammatory mediators, regulate the expression of inflammation-related genes, and reduce skin inflammation at the molecular level. In addition, it can promote the proliferation and differentiation of skin cells and accelerate the skin's self-repair process.

[0106] Chamomile extract, content of 0.03-1.5wt%;

[0107] It has the effects of soothing the skin, anti-allergic and repairing, can reduce skin sensitivity symptoms and enhance skin tolerance. For people with sensitive skin, the use of a composition containing chamomile extract can effectively reduce skin sensitivity by 45%. The flavonoids in chamomile extract have antioxidant and anti-inflammatory effects, which can reduce skin allergy symptoms such as redness, swelling and itching. At the same time, it can also regulate the skin's nerve conduction, relieve the skin's tension and make the skin more comfortable.

[0108] Grape seed extract, content of 0.02-1wt%;

[0109] It is rich in proanthocyanidins, has strong antioxidant capacity, can protect collagen from free radical damage, promote collagen synthesis, improve skin firmness and elasticity. After using a composition containing grape seed extract, the elasticity of the skin is increased by 28%. The proanthocyanidins in grape seed extract can activate the signal pathway related to collagen synthesis and promote fibroblasts to secrete collagen. In addition, it can also inhibit the activity of matrix metalloproteinases, reduce collagen degradation, and maintain the collagen content in the skin.

[0110] In the embodiments of the present invention, ergothioneine, glutathione, fish collagen, PQQ-pyrroloquinoline quinone disodium salt, coenzyme Q10, astaxanthin, ceramide and plant extracts work synergistically with each other to enhance the anti-aging effect from multiple aspects such as anti-oxidation, repairing the skin barrier, promoting cell metabolism, stimulating collagen synthesis, etc., and significantly improve the skin condition;

[0111] Different ingredients work together to form a comprehensive anti-aging system;

[0112] Antioxidant ingredients can remove free radicals, reduce damage to cells, and create a good environment for normal cell metabolism; ingredients that repair skin barrier can enhance the skin's self-protection ability, prevent water loss and the invasion of external harmful substances; ingredients that promote cell metabolism can accelerate cell renewal and improve skin vitality; ingredients that stimulate collagen synthesis can increase skin elasticity and firmness and reduce the formation of wrinkles.

[0113] In the embodiment of the present invention, safe and non-irritating auxiliary materials are also included, and the auxiliary materials include: moisturizers, emulsifiers, preservatives;

[0114] The moisturizer is glycerin, with a content of 2-10wt%, which is used to maintain the moisture content of the composition, enhance the moisturizing effect of the product, and keep the skin hydrated. Glycerin can absorb moisture in the air and provide continuous moisturizing effect for the skin.

[0115] The emulsifier is polysorbate-20, with a content of 0.5-3wt%, which is used to ensure that the ingredients are evenly dispersed to form a stable emulsion system, improve the stability and texture of the product. The emulsifier can make the oil phase and the water phase blend with each other to form a uniform emulsion, thereby improving the stability and feel of the product.

[0116] The preservative is potassium sorbate, with a content of 0.01-0.5wt%. On the premise of ensuring product safety, it prevents the growth of microorganisms and extends the shelf life of the product. The preservative can inhibit the growth of microorganisms such as bacteria and molds, ensuring the safety of the product during storage and use.

[0117] In the embodiments of the present invention, the composition can be used alone or with common auxiliary materials to prepare skin care product formulations such as creams, lotions, essences, moisturizers, and facial masks to meet different skin types and skin care needs:

[0118] Cream dosage form: By adding appropriate amounts of oil and wax ingredients, it has good occlusive properties and can provide deep nourishment to the skin. It is suitable for dry and mature skin to use at night. The oil in the cream can form a protective film on the skin surface to prevent moisture loss, while slowly releasing active ingredients into the skin to exert a long-lasting nourishing effect;

[0119] Emulsion dosage form: The texture is refreshing. By adjusting the emulsification system and oil content, it is easy to absorb and suitable for daily care of neutral and oily skin. The texture of the emulsion is light and can quickly penetrate into the skin to provide moisture and nutrition to the skin without causing greasy feeling.

[0120] Essence: Rich in high-concentration active ingredients, it uses small molecule penetration technology to accurately penetrate the bottom layer of the skin and play an efficient repair role. The active ingredients in the essence have been specially treated to quickly penetrate the skin barrier and reach the bottom layer of the skin to accurately repair skin problems;

[0121] Moisturizing water: With water as the base, adding appropriate amount of moisturizer and active ingredients, it can replenish moisture for the skin at any time and adjust the pH value of the skin. Moisturizing water can quickly replenish moisture for the skin, keep the skin moist, and at the same time adjust the acid-base balance of the skin and enhance the skin's resistance;

[0122] Mask liquid: With the help of the mask carrier, intensive nutrition delivery can be achieved in a short period of time, allowing the active ingredients to quickly penetrate the skin. The active ingredients in the mask liquid can quickly penetrate into the skin under the sealing effect of the mask, providing intensive nutrition care for the skin.

[0123] In the embodiments of the present invention, through safety and efficacy tests, the irritation index for sensitive skin groups is lower than 0.2. After 3 months of continuous use, skin wrinkles can be reduced by 35%, skin tone can be brightened by 2 degrees, and elasticity can be improved by 32%. It has excellent stability and safety, and can maintain the stability and effectiveness of active ingredients for a long time. After storage for 18 months at 4°C-40°C, humidity 30%-70% and light conditions, the loss rate of active ingredients is less than 8%.

[0124] In an embodiment of the present invention, ergothioneine, glutathione, fish collagen, PQQ-pyrroloquinoline quinone disodium salt, coenzyme Q10, astaxanthin, ceramide, plant extract and other active ingredients are uniformly mixed in proportion, and safe and non-irritating excipients or carriers are added to obtain the product through the following steps:

[0125] The emulsification step adopts advanced micro-emulsification technology and selects environmentally friendly, mild and efficient emulsifiers to ensure that the ingredients are evenly dispersed and form a stable micro-emulsion system, thereby improving the penetration efficiency of active ingredients. Micro-emulsification technology can make the emulsion particle size smaller and the distribution more even, thereby improving the penetration ability of active ingredients and making the product more effective.

[0126] The homogenization step uses high-pressure homogenization equipment to refine the particles to below the nanometer level, thereby improving the fineness and absorbency of the product. High-pressure homogenization equipment can refine the raw material particles, making them easier to be absorbed by the skin and improving the product's use effect.

[0127] The filling process is carried out in a sterile purification workshop, strictly controlling the microbial content to ensure product quality and safety. In the entire preparation process, environmental parameters such as temperature and humidity are strictly controlled to ensure that the activity of each ingredient is not affected. Sterile purification workshops and strict environmental control can ensure the quality and safety of the product and the activity and efficacy of each ingredient.

[0128] The embodiments of the present invention disclose preferred embodiments, but are not limited thereto. A person skilled in the art can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. However, as long as they do not deviate from the spirit of the present invention, they are all within the protection scope of the present invention.

Claims

1. An anti-aging composition containing ergothioneine, glutathione and fish collagen, characterized in that ； include: Ergothioneine: Ergothioneine is used as a natural antioxidant, and its content is 0.01-5wt%; Ergothioneine effectively inhibits tyrosinase activity, reduces melanin production, and protects DNA and proteins from oxidative damage by participating in the synergistic effect of the intracellular antioxidant enzyme system. In a simulated strong ultraviolet environment, the free radical content in the cells was reduced by 30% compared to skin cells that did not use ergothioneine products; Ergothioneine can also regulate cell signaling pathways, promote cell metabolism, maintain normal cell growth, reduce the release of inflammatory factors, play an anti-inflammatory role, provide auxiliary effects for the prevention of cardiovascular diseases, slow down skin aging and prevent ultraviolet damage, promote epidermal cell renewal, and accelerate the shedding of aging keratinocytes by 15%, keeping the skin young. Glutathione: Glutathione is a small molecular active oligopeptide, with a content of 0.001-3wt%; With the help of GSH-peroxidase-reductase system, it can accurately capture and remove harmful free radicals and lipid peroxides in the body; In skin tissue, it regulates the activity of enzymes related to melanin synthesis, prevents skin aging and pigment accumulation, and makes the skin appear naturally shiny; Glutathione can enhance the skin's barrier function and reduce water loss through the epidermis by 18%; By interacting with the water channel proteins in skin cells, it regulates the transport and retention of water, stabilizes the water balance inside the skin, improves the skin's moisturizing ability, and fundamentally improves the dry and dehydrated state of the skin; Collagen: Collagen is used as a high-quality collagen source, with a content of 0.1 to 10 wt%; Rich in various amino acids, it stimulates fibroblasts to secrete more collagen and elastic fibers; During the skin damage repair process, it attracts immune cells to gather and accelerate wound healing. For minor damaged skin, the skin repair rate can reach 70% within 3-5 days. Collagen can interact with integrins in the skin, enhance cell adhesion, improve skin elasticity and firmness, improve skin sagging, and make the skin feel firmer and smoother; PQQ-Pyrroloquinoline quinone disodium salt: PQQ-pyrroloquinoline quinone disodium salt has a unique function of activating mitochondria, and the content is 0.005-2wt%; PQQ-pyrroloquinoline quinone disodium salt can increase the number of mitochondria and improve their activity, providing sufficient power for cellular energy metabolism; In the process of energy metabolism, it promotes the synthesis of adenosine triphosphate (ATP) in cells, thereby enhancing cell vitality; After the treated cells were cultured in a medium containing PQQ-pyrroloquinoline quinone disodium salt, the cell energy metabolism level increased by 40%, accelerating skin metabolism, delaying the skin aging process, increasing the proliferation rate of skin cells by 1.5 times, and reducing the accumulation of aging cells; It can also regulate the redox balance within cells, enhance cells' resistance to environmental stress, and protect cells from external damage.

2. An anti-aging composition containing ergothioneine, glutathione and fish collagen according to claim 1, characterized in that, Also includes: Coenzyme Q10: content 0.01-2wt%; Coenzyme Q10 has an antioxidant effect, which can help remove free radicals, protect the integrity of cell membranes, enhance the skin's antioxidant capacity, help restore skin elasticity, and reduce wrinkles; In skin cell experiments, a combination with added coenzyme Q10 can slow down cell aging by 20%; Coenzyme Q10 can promote cell respiration, improve cell oxygen utilization, enhance cell energy production, and provide continuous power support for the skin; At the same time, it can also work synergistically with other antioxidant ingredients to form a powerful antioxidant network and enhance the overall antioxidant effect; Astaxanthin: content 0.001~1wt%; Astaxanthin is a highly effective antioxidant, with an antioxidant capacity 550 times that of vitamin E. It can effectively quench singlet oxygen, reduce free radical damage to the skin, improve skin dullness, and enhance skin brightness and color. After using a composition containing astaxanthin for 2 months, skin brightness can be increased by 30%. Astaxanthin has a unique molecular structure that enables it to penetrate the skin's multiple barriers and penetrate deep into the cells to exert an antioxidant effect. In addition, it can inhibit the activity of key enzymes in the production process of melanin, reduce the production of melanin, and improve the problem of skin dullness from the root. Ceramide: content of 0.05-3wt%; Ceramide can repair damaged skin barrier, enhance the skin's self-protection ability, reduce water loss, and maintain the skin's hydration. The composition containing ceramide can reduce the skin's water loss rate by 22%. Ceramide can interact with lipids in the skin's stratum corneum to form a tight lipid bilayer structure, restoring the integrity of the skin barrier. At the same time, it can also regulate the skin's immune response, enhance the skin's resistance, and reduce the irritation of the skin by external harmful substances.

3. An anti-aging composition containing ergothioneine, glutathione and fish collagen according to claim 2, characterized in that, Also includes plant extracts: green tea extract, chamomile extract, grape seed extract; Green tea extract, content of 0.05-2wt%; Rich in antioxidant ingredients such as tea polyphenols, it can remove free radicals, inhibit lipid peroxidation, reduce skin inflammation, and has antibacterial, anti-inflammatory and skin-soothing effects. In the inflammatory skin model experiment, the use of a composition containing green tea extract can reduce inflammatory factors by 40%. The tea polyphenols in green tea extract can inhibit the release of inflammatory mediators, regulate the expression of inflammation-related genes, and reduce skin inflammation at the molecular level. In addition, it can promote the proliferation and differentiation of skin cells and accelerate the skin's self-repair process. Chamomile extract, content of 0.03-1.5wt%; It has the effects of soothing the skin, anti-allergic and repairing, can reduce skin sensitivity symptoms and enhance skin tolerance. For people with sensitive skin, the use of a composition containing chamomile extract can effectively reduce skin sensitivity by 45%. The flavonoids in chamomile extract have antioxidant and anti-inflammatory effects, which can reduce skin allergy symptoms such as redness, swelling and itching. At the same time, it can also regulate the skin's nerve conduction, relieve the skin's tension and make the skin more comfortable. Grape seed extract, content of 0.02-1wt%; It is rich in proanthocyanidins, has strong antioxidant capacity, can protect collagen from free radical damage, promote collagen synthesis, improve skin firmness and elasticity. After using a composition containing grape seed extract, the elasticity of the skin is increased by 28%. The proanthocyanidins in grape seed extract can activate the signal pathway related to collagen synthesis and promote fibroblasts to secrete collagen. In addition, it can also inhibit the activity of matrix metalloproteinases, reduce collagen degradation, and maintain the collagen content in the skin.

4. An anti-aging composition containing ergothioneine, glutathione and fish collagen according to claim 3, characterized in that, Ergothioneine, glutathione, fish collagen, PQQ-pyrroloquinoline quinone disodium salt, coenzyme Q10, astaxanthin, ceramide and plant extracts work synergistically to enhance anti-aging effects from multiple aspects, such as anti-oxidation, repairing skin barrier, promoting cell metabolism, stimulating collagen synthesis, etc., and significantly improve skin condition; Different ingredients work together to form a comprehensive anti-aging system; Antioxidant ingredients can remove free radicals, reduce damage to cells, and create a good environment for normal cell metabolism; ingredients that repair skin barrier can enhance the skin's self-protection ability, prevent water loss and the invasion of external harmful substances; ingredients that promote cell metabolism can accelerate cell renewal and improve skin vitality; ingredients that stimulate collagen synthesis can increase skin elasticity and firmness and reduce the formation of wrinkles.

5. An anti-aging composition containing ergothioneine, glutathione and fish collagen according to claim 4, characterized in that, Also included are safe, non-irritating excipients, including: Moisturizers, emulsifiers, preservatives; The moisturizer is glycerin, with a content of 2-10wt%, which is used to maintain the moisture content of the composition, enhance the moisturizing effect of the product, and keep the skin hydrated. Glycerin can absorb moisture in the air and provide continuous moisturizing effect for the skin. The emulsifier is polysorbate-20, with a content of 0.5-3wt%, which is used to ensure that the ingredients are evenly dispersed to form a stable emulsion system, improve the stability and texture of the product. The emulsifier can make the oil phase and the water phase blend with each other to form a uniform emulsion, thereby improving the stability and feel of the product. The preservative is potassium sorbate, with a content of 0.01-0.5wt%. On the premise of ensuring product safety, it prevents the growth of microorganisms and extends the shelf life of the product. The preservative can inhibit the growth of microorganisms such as bacteria and molds, ensuring the safety of the product during storage and use.

6. An anti-aging composition containing ergothioneine, glutathione and fish collagen according to claim 5, characterized in that, The composition can be used alone or with common auxiliary materials to make skin care products such as creams, lotions, essences, moisturizing water, and facial masks to meet different skin types and skin care needs: Cream dosage form: By adding appropriate amounts of oil and wax ingredients, it has good occlusive properties and can provide deep nourishment to the skin. It is suitable for dry and mature skin to use at night. The oil in the cream can form a protective film on the skin surface to prevent moisture loss, while slowly releasing active ingredients into the skin to exert a long-lasting nourishing effect; Emulsion dosage form: The texture is refreshing. By adjusting the emulsification system and oil content, it is easy to absorb and suitable for daily care of neutral and oily skin. The texture of the emulsion is light and can quickly penetrate into the skin to provide moisture and nutrition to the skin without causing greasy feeling. Essence: Rich in high-concentration active ingredients, it uses small molecule penetration technology to accurately penetrate the bottom layer of the skin and play an efficient repair role. The active ingredients in the essence have been specially treated to quickly penetrate the skin barrier and reach the bottom layer of the skin to accurately repair skin problems; Moisturizing water: With water as the base, adding appropriate amount of moisturizer and active ingredients, it can replenish moisture for the skin at any time and adjust the pH value of the skin. Moisturizing water can quickly replenish moisture for the skin, keep the skin moist, and at the same time adjust the acid-base balance of the skin and enhance the skin's resistance; Mask liquid: With the help of the mask carrier, intensive nutrition delivery can be achieved in a short period of time, allowing the active ingredients to quickly penetrate the skin. The active ingredients in the mask liquid can quickly penetrate into the skin under the sealing effect of the mask, providing intensive nutrition care for the skin.

7. An anti-aging composition containing ergothioneine, glutathione and fish collagen according to claim 6, characterized in that: Through safety and efficacy tests, the irritation index for people with sensitive skin is less than 0.

2. After 3 months of continuous use, skin wrinkles can be reduced by 35%, skin tone can be brightened by 2 degrees, and elasticity can be increased by 32%. It has excellent stability and safety, and can maintain the stability and effectiveness of active ingredients for a long time. After storage for 18 months at 4℃-40℃, humidity 30%-70% and light conditions, the active ingredient loss rate is less than 8%.

8. An anti-aging composition containing ergothioneine, glutathione and fish collagen according to claim 7, characterized in that: Ergothioneine, glutathione, fish collagen, PQQ-pyrroloquinoline quinone disodium salt, coenzyme Q10, astaxanthin, ceramide, plant extracts and other active ingredients are mixed evenly in proportion, and safe and non-irritating excipients or carriers are added to obtain the product through the following steps: The emulsification step adopts advanced micro-emulsification technology and selects environmentally friendly, mild and efficient emulsifiers to ensure that the ingredients are evenly dispersed and form a stable micro-emulsion system, thereby improving the penetration efficiency of active ingredients. Micro-emulsification technology can make the emulsion particle size smaller and the distribution more even, thereby improving the penetration ability of active ingredients and making the product more effective. The homogenization step uses high-pressure homogenization equipment to refine the particles to below the nanometer level, thereby improving the fineness and absorbency of the product. High-pressure homogenization equipment can refine the raw material particles, making them easier to be absorbed by the skin and improving the product's use effect. The filling process is carried out in a sterile purification workshop, strictly controlling the microbial content to ensure product quality and safety. In the entire preparation process, environmental parameters such as temperature and humidity are strictly controlled to ensure that the activity of each ingredient is not affected. Sterile purification workshops and strict environmental control can ensure the quality and safety of the product and the activity and efficacy of each ingredient.