Composition with anti-aging function and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and discloses a composition with an anti-aging function and a preparation method thereof.The composition with the anti-aging function is prepared from deionized water, a natural plant extracting solution, natural essential oil, an anti-aging component, an antibacterial moisturizing component, an emulsifying agent and a thickening agent. The anti-aging component is prepared by enabling oat beta glucan to react with arginine and then performing self-assembly with palmitoyl pentapeptide-4 with negative electricity under the action of electrostatic adsorption, and the antibacterial moisturizing component is prepared by enabling inulin to react with plant organic acid. The prepared composition has excellent anti-aging and antibacterial moisturizing effects, can be prepared into essence, and can delay skin aging, stimulate collagen generation, improve the skin state, lock moisture, deeply moisturize the skin, enhance skin barrier, inhibit bacteria and make the skin not prone to redness.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition with anti-aging functionality and a preparation method thereof. Background Art

[0002] Nowadays, people regard delicate and shiny skin as beauty, but skin aging is an inevitable problem. Skin aging is mainly related to age, metabolic disorders, changes in genetic material, free radical damage to cells, light, chemistry, physics, mechanics and other factors. It is mainly manifested as skin aging, dullness, decreased elasticity, spots due to pigmentation, loose skin and wrinkles, etc. Therefore, anti-aging skin products have become people's choice.

[0003] As anti-aging cosmetics, it is essential to be able to stimulate collagen production and improve the firmness and elasticity of the skin. Of course, the antibacterial and moisturizing effects are also indispensable. Once the skin is dehydrated, it will cause the skin to become dry and tight, its elasticity will decrease, and the protective barrier function will be weakened, leading to skin sensitivity, water-oil imbalance, bacteria breeding on the skin surface causing skin redness, acne, spots, acne, etc., as well as an increase in the number of exfoliated cells in the epidermis of the skin, increased texture, and skin aging. Therefore, the antibacterial and moisturizing effects of skin care products can help the skin lock in facial moisture, deeply moisturize, keep the skin's moisture balance, enhance the skin's barrier function and the ability to absorb the effective ingredients in the skin care products, so that the skin is not prone to redness, sensitivity, and acne. The present invention aims to prepare an essence with anti-aging, moisturizing and antibacterial effects to delay skin aging, which has very important practical significance.

[0004] The patent with publication number CN114917175B discloses an anti-aging composition, which is composed of 10% by weight of pearl fermentation liquid and 3% by weight of essential oil, wherein the essential oil is a mixture of angelica essential oil and licorice essential oil in a weight ratio of 1:1, and the rest is a cosmetic matrix. The composition can significantly reduce the lipofuscin content of skin tissue, improve antioxidant capacity, remove free radicals in the body, reduce lipid peroxidation damage, and has the effect of delaying aging. It has a better anti-aging effect than the pearl fermentation liquid and the essential oil alone. However, the composition still has shortcomings in practical applications, ignoring the aging caused by increased shedding of skin epidermal cells due to skin dehydration and the skin surface is prone to bacteria breeding after the skin barrier is damaged, resulting in skin redness, and has no moisturizing and antibacterial effects. Therefore, the present invention provides a composition with anti-aging functionality, which has both anti-aging and antibacterial moisturizing effects. Summary of the invention

[0005] The purpose of the present invention is to provide a composition with anti-aging functionality and a preparation method thereof, to solve the following technical problems:

[0006] (1) Solve the problem of insufficient anti-aging efficacy of the composition;

[0007] (2) The problem of insufficient antibacterial and moisturizing effects of the composition is solved.

[0008] The purpose of the present invention can be achieved by the following technical solutions:

[0009] A composition with anti-aging functionality comprises the following raw materials in parts by weight: 120-150 parts of deionized water, 80-120 parts of natural plant extracts, 15-25 parts of natural essential oils, 5-10 parts of anti-aging components, 8-12 parts of antibacterial and moisturizing components, 1-3 parts of emulsifiers, and 1-4 parts of thickeners.

[0010] Furthermore, the natural plant extract is any one of aloe extract, tea extract, and seaweed extract; the natural essential oil is rose essential oil, lavender essential oil, or linseed oil; the emulsifier is sorbitan laurate or sorbitan stearate; and the thickener is any one of carbomer, high molecular weight cellulose, and xanthan gum.

[0011] Furthermore, the preparation method of the anti-aging component comprises the following steps:

[0012] Step 1: add oat beta glucan to deionized water, stir to dissolve, add arginine and catalyst, stir evenly, heat to 80-90°C, react for 1.5-2.5h, and distill under reduced pressure to obtain modified oat beta glucan;

[0013] Step 2: Use sodium hydroxide to adjust the pH value of deionized water, add modified oat β-glucan and palmitoyl pentapeptide-4 and stir to form a mixed solution, place the mixed solution in a water bath oscillator, set the water bath temperature to 30-40°C, set the rotation speed to 80-100 r / min, and oscillate for 8-10 hours to form a complex to obtain an anti-aging component.

[0014] By adopting the above technical scheme, under the action of a catalyst, a large number of active hydroxyl groups in oat beta glucan can undergo an esterification reaction with the active carboxyl groups in the arginine structure to obtain modified oat beta glucan. The modified oat beta glucan is positively charged at physiological pH, while palmitoyl pentapeptide-4 is negatively charged at physiological pH. Therefore, the two can self-assemble under the action of electrostatic adsorption to form a complex to obtain an anti-aging component.

[0015] Furthermore, in step 1, the catalyst is 4-dimethylaminopyridine and N,N-dicyclohexylcarbodiimide.

[0016] Furthermore, in step 2, the pH value is 7.35-7.45.

[0017] Furthermore, the preparation method of the antibacterial moisturizing component is as follows:

[0018] The inulin is dissolved in deionized water and stirred evenly to prepare an inulin solution, the inulin solution is heated in a water bath at 30-45°C, plant organic acid, 4-dimethylaminopyridine and N,N-dicyclohexylcarbodiimide are added, the reaction is carried out for 1-2 hours, and the solution is dialyzed, purified and dried to obtain an antibacterial and moisturizing component.

[0019] By adopting the above technical scheme, under the action of a catalyst, the active hydroxyl groups contained in inulin can undergo an esterification reaction with the active carboxyl groups in the plant organic acid structure to produce an antibacterial and moisturizing component.

[0020] Furthermore, the mass fraction of the inulin liquid is 15%-35%.

[0021] Furthermore, the plant organic acid is any one of gallic acid, citric acid or tannic acid.

[0022] A method for preparing a composition with anti-aging functionality comprises the following steps:

[0023] The first step is to add deionized water, natural plant extract, natural essential oil, emulsifier and thickener into the water phase pot, stir evenly, heat to 50-60°C, set the homogenization speed to 2500-3000r / min, homogenize for 3-5min, disperse evenly, and obtain a mixture;

[0024] The second step is to add the mixture, anti-aging components, and antibacterial and moisturizing components into the water phase pot, heat it to 40-50°C, stir for 10-15 minutes, and disperse evenly to obtain a composition with anti-aging functionality.

[0025] Beneficial effects of the present invention:

[0026] (1) The anti-aging component prepared by the present invention has an arginine structure and contains a guanidine group. After reacting with oat β-glucan, the modified oat β-glucan can be positively charged under physiological pH conditions, and self-assemble with palmitoyl pentapeptide-4, which is negatively charged under physiological pH, to form a complex through electrostatic adsorption. The palmitoyl pentapeptide-4 in the anti-aging component can stimulate fibroblasts to express collagen, enhance skin elasticity, increase skin thickness, improve skin quality, and increase skin moisture retention, so that the skin remains hydrated. It is mild and non-irritating and suitable for all skin types. At the same time, the oat β-glucan in the anti-aging component has good skin permeability, so that arginine and palmitoyl pentapeptide-4 can penetrate deeply into the skin and exert their effects. The oat β-glucan can form a thin film on the skin epidermis, so that moisture can enter the skin but the moisture in the skin cannot penetrate out, thereby maintaining the moisture content in the skin. It can synergistically moisturize with the antibacterial and moisturizing component, thereby improving the anti-aging and moisturizing effects of the composition with anti-aging functionality.

[0027] (2) The antibacterial and moisturizing component prepared by the present invention has plant organic acids, which have antibacterial effects and can control skin inflammation and inhibit the growth and reproduction of microorganisms. No additional preservatives are needed to increase the stability of the composition with anti-aging functions and prolong its shelf life. The inulin in the antibacterial and moisturizing component has excellent hygroscopicity and can absorb moisture by combining with free water, increase the water content of the stratum corneum, reduce water activity, and prevent water evaporation. The traditional moisturizers glycerin and propylene glycol will not absorb moisture from the inner layer of the skin when the air humidity is low, causing the skin to become drier and less water-rich. At the same time, inulin has excellent dispersibility, spreadability and thickening effects, so that the composition with anti-aging functions has a better skin feel and better moisturizing and antibacterial effects.

[0028] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0030] Figure 1 is an infrared spectrum of the modified oat beta glucan prepared in an embodiment of the present invention;

[0031] Figure 2 This is an infrared spectrum of the antibacterial and moisturizing component prepared in the embodiment of the present invention. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Example 1

[0034] 1. Preparation of anti-aging components

[0035] Step 1: add 3.5 g of oat beta glucan into deionized water, stir to dissolve, then add 0.5 g of arginine, 0.2 g of 4-dimethylaminopyridine and 0.3 g of N,N-dicyclohexylcarbodiimide, stir evenly, heat to 85°C, react for 2 h, and distill under reduced pressure to obtain modified oat beta glucan;

[0036] Step 3: Use sodium hydroxide to adjust the pH of deionized water to 7.4, add 3g of modified oat β-glucan and 3g of palmitoyl pentapeptide-4 and stir to form a mixed solution, place the mixed solution in a water bath oscillator, set the temperature to 35°C, set the speed to 90r / min, and oscillate for 8h to form a complex to obtain an anti-aging component.

[0037] The samples were prepared by potassium bromide tableting method, and the modified oat β-glucan was tested by infrared spectrometer. Figure 1 As shown, the spectral wavenumber test range is 4000cm -1 -500cm -1 , the analysis shows that in the infrared spectrum of modified oat β-glucan, 3428cm -1 The absorption peak of amine group appeared at 1720cm -1 The absorption peak of C=O in the ester group appeared at 1645 cm -1 The absorption peak of C=N appeared at 1040cm -1 The absorption peak of the ether bond -COC appeared at

[0038] 2. Preparation of antibacterial and moisturizing components

[0039] 3 g of inulin was dissolved in deionized water and stirred evenly to prepare an inulin solution with a mass fraction of 20%. The inulin solution was heated in a water bath at 30°C, 1 g of gallic acid, 0.3 g of 4-dimethylaminopyridine and 0.5 g of N,N-dicyclohexylcarbodiimide were added, the reaction was carried out for 1.5 h, and the mixture was dialyzed, purified and dried to obtain an antibacterial and moisturizing component.

[0040] The samples were prepared by potassium bromide tableting method, and infrared testing of antibacterial and moisturizing components was performed using Fourier transform infrared spectrometer. Figure 2 As shown, the spectral wavenumber test range is 4000cm -1 -500cm -1 According to the analysis, in the infrared spectrum of antibacterial and moisturizing components, 3025cm -1 The absorption peak of CH in the benzene ring appeared at 1731 cm -1 The absorption peak of C=O in the ester group appeared at 1034 cm -1 The absorption peak of ether bond -COC appeared at 935cm -1 The absorption peak of the fructosylfuran ring appeared at

[0041] 3. Preparation of a composition with anti-aging functionality

[0042] Step 1: Add 120 g of deionized water, 80 g of aloe extract, 15 g of rose essential oil, 1 g of sorbitan laurate, and 1 g of xanthan gum into an aqueous phase pot, stir evenly, heat to 50° C., set the homogenization speed to 2500 r / min, homogenize for 3 min, and disperse evenly to obtain a mixture;

[0043] Step 2: Add the mixture, 5g of anti-aging component and 8g of antibacterial and moisturizing component into the water phase pot, heat to 40°C, stir for 10 minutes, and disperse evenly to obtain a composition with anti-aging functionality.

[0044] Example 2

[0045] 1. Preparation of a composition with anti-aging functionality

[0046] Step 1: Add 130 g of deionized water, 100 g of aloe extract, 20 g of rose essential oil, 2 g of sorbitan laurate, and 2 g of xanthan gum into an aqueous phase pot, stir evenly, heat to 55° C., set the homogenization speed to 2750 r / min, homogenize for 4 min, and disperse evenly to obtain a mixture;

[0047] In the second step, add the mixture, 6 g of the anti-aging component prepared in Example 1 of the present invention, and 10 g of the antibacterial and moisturizing component prepared in Example 1 of the present invention into the water phase pot, heat to 45° C., stir for 12 minutes, and disperse evenly to obtain a composition with anti-aging functionality.

[0048] Example 3

[0049] 1. Preparation of a composition with anti-aging functionality

[0050] Step 1: Add 140 g of deionized water, 110 g of aloe extract, 22 g of rose essential oil, 2.5 g of sorbitan laurate, and 3 g of xanthan gum into the water phase pot, stir evenly, heat to 60°C, set the homogenization speed to 2900 r / min, homogenize for 5 min, disperse evenly, and obtain a mixture;

[0051] Step 2: Add the mixture, 8 g of the anti-aging component prepared in Example 1 of the present invention, and 11 g of the antibacterial and moisturizing component prepared in Example 1 of the present invention into the water phase pot, heat to 50° C., stir for 15 minutes, and disperse evenly to obtain a composition with anti-aging functionality.

[0052] Example 4

[0053] 1. Preparation of a composition with anti-aging functionality

[0054] Step 1: Add 150 g of deionized water, 120 g of aloe extract, 25 g of rose essential oil, 3 g of sorbitan laurate, and 4 g of xanthan gum into an aqueous phase pot, stir evenly, heat to 60° C., set the homogenization speed to 3000 r / min, homogenize for 5 min, and disperse evenly to obtain a mixture;

[0055] In the second step, add the mixture, 10 g of the anti-aging component prepared in Example 1 of the present invention, and 12 g of the antibacterial and moisturizing component prepared in Example 1 of the present invention into the water phase pot, heat to 50° C., stir for 15 minutes, and disperse evenly to obtain a composition with anti-aging functionality.

[0056] Comparative Example 1

[0057] 1. Preparation of the composition

[0058] Step 1: Add 130 g of deionized water, 100 g of aloe extract, 20 g of rose essential oil, 2 g of sorbitan laurate, and 2 g of xanthan gum into an aqueous phase pot, stir evenly, heat to 55° C., set the homogenization speed to 2750 r / min, homogenize for 4 min, and disperse evenly to obtain a mixture;

[0059] Step 2: Add the mixture and 6 g of the anti-aging component prepared in Example 1 of the present invention into a water phase pot, heat to 45° C., stir for 12 minutes, and disperse evenly to obtain a composition with anti-aging functionality.

[0060] Comparative Example 2

[0061] 1. Preparation of the composition

[0062] Step 1: Add 130 g of deionized water, 100 g of aloe extract, 20 g of rose essential oil, 2 g of sorbitan laurate, and 2 g of xanthan gum into an aqueous phase pot, stir evenly, heat to 55° C., set the homogenization speed to 2750 r / min, homogenize for 4 min, and disperse evenly to obtain a mixture;

[0063] Step 2: Add the mixture and 10 g of the antibacterial and moisturizing component prepared in Example 1 of the present invention into a water phase pot, heat to 45° C., stir for 12 minutes, and disperse evenly to obtain a composition with anti-aging functionality.

[0064] Comparative Example 3

[0065] 1. Preparation of the composition

[0066] Step 1: Add 130 g of deionized water, 100 g of aloe extract, 20 g of rose essential oil, 2 g of sorbitan laurate, and 2 g of xanthan gum into an aqueous phase pot, stir evenly, heat to 55° C., set the homogenization speed to 2750 r / min, homogenize for 4 min, and disperse evenly to obtain a mixture;

[0067] Step 2: Add the mixture into the water phase pot, heat to 45°C, stir for 12 minutes, and disperse evenly to obtain a composition with anti-aging functionality.

[0068] The compositions prepared in Examples 1 to 4 of the present invention and Comparative Examples 1 to 3 were tested as follows:

[0069] a. Irritation test

[0070] According to the skin irritation / corrosion test of the cosmetic hygiene specification, 70 healthy rats of the same weight were selected, 10 rats in each group, and divided into 7 groups. The hair at the same position on the back of the rats was shaved, and the composition was applied. The skin phenomenon at that position was recorded within 24-48 hours. The results are as follows:

[0071] Table 1 Skin irritation test results

[0072] Group Phenomenon after 24 hours Phenomenon after 48 hours Example 1 No obvious skin symptoms No obvious skin symptoms Example 2 No obvious skin symptoms No obvious skin symptoms Example 3 No obvious skin symptoms No obvious skin symptoms Example 4 No obvious skin symptoms No obvious skin symptoms Comparative Example 1 No obvious skin symptoms No obvious skin symptoms Comparative Example 2 No obvious skin symptoms No obvious skin symptoms Comparative Example 3 No obvious skin symptoms No obvious skin symptoms

[0073] It can be seen from the above table that the composition prepared by the present invention has safe ingredients, is non-irritating, does not cause allergies, and can be widely used.

[0074] b. Inoculate E. coli into liquid culture medium, culture at 37°C, 220 r / min for 24 h, and dilute to 10 with phosphate buffer. 4 -10 6 CFU / mL bacterial suspension, add 1g of the composition prepared in Example 1-Example 4 and Comparative Example 1-Comparative Example 3 to the bacterial suspension and evenly coat and mix, record the absorbance at 450nm at 0h, culture at 37°C for 48h as a sample group, and set a blank control group without adding the composition, record the absorbance at 450nm, and calculate using the formula: Antibacterial rate (%) = [(control group OD value-sample group OD value) / control group OD value] × 100%, the results are as follows:

[0075] Table 2 Antibacterial effect

[0076] Group Antibacterial rate Example 1 99.2％ Example 2 99.6％ Example 3 99.3％ Example 4 99.5％ Comparative Example 1 74.6％ Comparative Example 2 98.7％ Comparative Example 3 69.2％

[0077] As can be seen from the above table, the compositions prepared in Examples 1 to 4 have excellent antibacterial effects, while Comparative Examples 1 and 3 do not add antibacterial moisturizing components and cannot utilize inulin-grafted plant organic acids to inhibit bacterial growth and reproduction, and thus have poor antibacterial effects.

[0078] c. 70 healthy rats of the same weight were selected, 10 rats in each group, and divided into 7 groups. The hair at the same position on the back of the rats was shaved off, and the composition was applied. The initial skin moisture value of the rats was tested with a CM825 skin moisture tester, and then the moisture value of the skin at the application site was tested 30 minutes, 60 minutes, and 90 minutes after the composition was applied. The average value was taken, and the skin water content growth rate was calculated according to the formula [(skin moisture value after application-skin moisture value before application) / skin moisture value before application × 100%]. The results are as follows:

[0079] Table 3 Growth rate of skin water content

[0080] Group 30min 60min 90min Example 1 59.4％ 58.1％ 56.9％ Example 2 60.2％ 59.3％ 58.4％ Example 3 59.6％ 58.4％ 57.3％ Example 4 59.8％ 58.7％ 54.6％ Comparative Example 1 39.1％ 38.2％ 37.3％ Comparative Example 2 57.1％ 56.4％ 55.5％ Comparative Example 3 38.2％ 36.9％ 35.1％

[0081] As can be seen from the above table, the compositions prepared in Examples 1 to 4 of the present invention have excellent moisturizing effects. Comparative Examples 1 and 3 do not add antibacterial moisturizing components, and cannot utilize the hygroscopicity of inulin to combine with free water, absorb moisture, and have moisturizing effects, so the test results are poor.

[0082] d. Fibroblasts were cultured in DMEM medium containing 10% FBS and uniformly inoculated on a 48-well culture plate. After the cells attached, the medium was exchanged with DEME medium containing 0.25% FBS and magnesium ascorbyl phosphate, and the compositions prepared in Examples 1 to 4 and Comparative Examples 1 to 3 were added. After culturing in a carbon dioxide incubator for 2 days, the culture supernatant was recovered, centrifuged, and the content of soluble collagen in the supernatant was determined. The results are shown in the following table:

[0083] Table 4 Soluble collagen content

[0084]

[0085]

[0086] As can be seen from the above table, the compositions prepared in Examples 1 to 4 of the present invention have excellent anti-aging effects on stimulating collagen production. Comparative Examples 2 and 3 do not add anti-aging components, and cannot utilize modified oat β-glucan to electrostatically adsorb palmitoyl pentapeptide-4 to stimulate fibroblasts to express collagen, so the test results are poor.

[0087] The above contents are merely examples and explanations of the concept of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the concept of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. A composition with anti-aging functionality, characterized in that: The invention comprises the following raw materials in parts by weight: 120-150 parts of deionized water, 80-120 parts of natural plant extract, 15-25 parts of natural essential oil, 5-10 parts of anti-aging components, 8-12 parts of antibacterial and moisturizing components, 1-3 parts of emulsifiers and 1-4 parts of thickeners.

2. The composition with anti-aging functionality according to claim 1, characterized in that: The natural plant extract is any one of aloe extract, tea extract and seaweed extract; the natural essential oil is rose essential oil, lavender essential oil or linseed oil; the emulsifier is sorbitan laurate or sorbitan stearate; the thickener is any one of carbomer, high molecular weight cellulose and xanthan gum.

3. The composition with anti-aging function according to claim 1, characterized in that: The preparation method of the anti-aging component comprises the following steps: Step 1: add oat beta glucan to deionized water, stir to dissolve, add arginine and catalyst, stir evenly, heat to 80-90°C, react for 1.5-2.5h, and distill under reduced pressure to obtain modified oat beta glucan; Step 2: Use sodium hydroxide to adjust the pH value of deionized water, add modified oat β-glucan and palmitoyl pentapeptide-4 and stir to form a mixed solution, place the mixed solution in a water bath oscillator, set the water bath temperature to 30-40°C, set the rotation speed to 80-100 r / min, and oscillate for 8-10 hours to form a complex to obtain an anti-aging component.

4. The composition with anti-aging function according to claim 3, characterized in that: In step 1, the catalyst is 4-dimethylaminopyridine and N,N-dicyclohexylcarbodiimide.

5. The composition with anti-aging function according to claim 3, characterized in that: In step 2, the pH value is 7.35-7.

45.

6. The composition with anti-aging function according to claim 1, characterized in that: The preparation method of the antibacterial moisturizing component is as follows: The inulin is dissolved in deionized water and stirred evenly to prepare an inulin solution, the inulin solution is heated in a water bath at 30-45°C, plant organic acid, 4-dimethylaminopyridine and N,N-dicyclohexylcarbodiimide are added, the reaction is carried out for 1-2 hours, dialyzed, purified and dried to obtain an antibacterial and moisturizing component.

7. The composition with anti-aging function according to claim 6, characterized in that: The mass fraction of the inulin liquid is 15%-35%.

8. The composition with anti-aging function according to claim 6, characterized in that: The plant organic acid is any one of gallic acid, citric acid or tannic acid.

9. A method for preparing the composition with anti-aging functionality as claimed in claim 1, characterized in that: The following steps are involved: The first step is to add deionized water, natural plant extract, natural essential oil, emulsifier and thickener into the water phase pot, stir evenly, heat to 50-60°C, set the homogenization speed to 2500-3000r / min, homogenize for 3-5min, disperse evenly, and obtain a mixture; The second step is to add the mixture, anti-aging components, and antibacterial and moisturizing components into the water phase pot, heat it to 40-50°C, stir for 10-15 minutes, and disperse evenly to obtain a composition with anti-aging functionality.

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