Oil-control repairing composition and application thereof

Abstract

The invention provides an oil-control repairing composition and application thereof.The oil-control repairing composition comprises pyridoxine tri-hexylcaprate, a hyperforin perforatum extract, a phellinus igniarius extract and a walnuts beer extract, and the oil-control effect is achieved by inhibiting PPAR gamma expression, RXR alpha expression and 5-alpha reductase activity of sebaceous gland cells and promoting RAR alpha expression; the repairing effect is achieved by promoting FLG expression. The composition is simple in raw material composition, achieves remarkable oil control and repair effects under the condition of lower additive amount by utilizing the synergistic effect of the composition, can be used for preparing skin care products and inhibiting sebaceous gland from secreting grease, and meanwhile, gives consideration to skin barrier repair.

Description

Technical Field

[0001] The present invention belongs to the field of skin care products, and in particular, relates to an oil control and repairing composition and application thereof. Background Art

[0002] Skin health has always been one of the focal issues that people pay attention to. In modern society, consumers are easily troubled by oily skin and damaged skin barrier due to high pressure in life, staying up late, irregular diet and other reasons.

[0003] Sebum is produced by the sebaceous glands of the hair follicles. The periphery of mature sebaceous glands is composed of cells with active mitosis. During the differentiation process, they gradually move toward the center and gradually lose their mitotic activity and accumulate lipid droplets. They eventually reach the center and disintegrate, releasing their contents to form sebum. This process is controlled by various paracrine, endocrine and neurotransmitters and regulated by various receptors related to sebaceous cell expression. When sebum is secreted too much, it is easy to cause pores to enlarge, which in turn causes water loss. After the water content decreases, in order to prevent the skin from being disturbed, the sebaceous glands will further release more oil, entering a vicious cycle, and eventually causing damage to the skin barrier. Therefore, when selecting oil-control raw materials, it is also necessary to pay attention to skin barrier repair at the same time.

[0004] However, although there are many oil control products and repair products on the market, these products often only address one of the issues; or they use a dozen or even more substances to achieve the purpose of oil control and repair. The mechanism of this combination is not clear enough, and the production process is complicated and the cost is too high.

[0005] Patent CN118370710A discloses a method for preparing and using an oil-control composition for both hair and skin, which achieves the effect of oil control by inhibiting the IL-6 inflammatory factor. The composition specifically includes the following components: 1-5% apple root extract, 1-5% orientalis leaf extract, 0.5-2% sophora flavescens root extract, 50-70% 1,3-butanediol, and 20-40% water. The composition also studies the inhibition of inflammatory factors and evaluates the efficacy results in humans. Patent CN118267323A discloses an oil control and repair composition, which uses long-term stable small molecule peptides to achieve oil control and repair, and has carried out stability verification and in vitro efficacy tests. The composition is specifically composed of the following ingredients: dipeptide-15 3-4.5wt%, oligopeptide-1 0.1-0.2wt%, dianthus 0.002-0.01wt%, Tibetan mushroom extract 0.005-0.015wt%, PEG-40 hydrogenated castor oil 1-2wt%, hexanediol 0.2-1wt%, pentanediol 1-5wt%, cellulose 0.1-0.5wt%, pH adjuster to adjust the pH of the composition to 4-7, and the balance is water. Patent CN113425650A discloses an oil control and repair composition, which includes the following components in weight percentage: pyridoxine 0.0001-20%, 10-hydroxydecanoic acid 0.0001-20%, betaine salicylate 0.0001-20%, white willow bark extract 0.0001-20%, DNA sodium 0.0001-20%, and the balance is water and / or solvent, and its human efficacy is evaluated by indicators such as the amount of oil produced.

[0006] Therefore, it is necessary to find a skin care product which can not only control oil and repair, but also has a simple composition and a low additive content. Summary of the invention

[0007] In view of the deficiencies of the existing technology, the present invention provides an oil control and repair composition and its application, the composition includes pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal pore fungus extract and walnut extract, and achieves oil control effect by inhibiting PPARγ expression, RXRα expression, 5-α reductase activity of sebaceous gland cells and promoting RARα expression of sebaceous gland cells, and significantly promotes FLG expression to achieve repair effect. The raw material composition of the composition is simple, and the synergistic effect of the composition is utilized to achieve significant oil control and repair effect at a lower addition amount, and can be used to prepare skin care products, inhibit sebaceous glands from secreting oil, and take into account skin barrier repair.

[0008] An oil control and repairing composition, characterized by comprising pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal pore fungus extract and Juglans burrardii extract.

[0009] Pyridoxine tri-hexyldecanoate is a newly registered raw material with the advantages of good oil solubility, lipophilicity and skin-friendliness. In addition, studies have shown that significant effects can be observed in repair and oil control at an addition amount of 4%, and it has considerable prospects for use.

[0010] Hypericum perforatum extract comes from Hypericum perforatum, a perennial herb of the genus Hypericum of the family Garciniaaceae. It is rich in flavonoids. At an addition amount of 1%, it can achieve the purpose of oil control by inhibiting 5-α reductase, pAkt / pErk1 / 2 pathway and SREBP-1 / PPAR-γ pathway.

[0011] The medicinal pore fungus extract is derived from the medicinal pore fungus. Literature indicates that it can exhibit excellent moisturizing and oil-control capabilities at a 5% addition amount.

[0012] Walnut extract is a plant-derived raw material obtained from the fruit of the Marula tree, which is native to Africa and has excellent moisturizing and repairing abilities.

[0013] Furthermore, the Hypericum perforatum extract includes one or more of Hypericum perforatum extract, Hypericum perforatum flower extract, Hypericum perforatum twig extract, Hypericum perforatum leaf extract, Hypericum perforatum stem extract, and Hypericum perforatum oil; the Juglans walnut extract includes one or more of Juglans walnut seed oil and Juglans walnut fruit extract.

[0014] Furthermore, the mass ratio of pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal porphyra extract and Juglans burrardii extract is (0.005-0.5): (0.001-1): (0.1-5): (0.001-0.5).

[0015] In some embodiments, when the composition is used to achieve a certain effect, the amount of each component added is lower than the amount required to achieve a certain effect when each component is used alone, which greatly reduces costs and increases efficiency.

[0016] Furthermore, the present invention provides a skin care product, which contains the above composition.

[0017] In some ways, skin care products can be divided into creams, lotions, aqueous solutions, gels, oils, powders, block powders or solids, muds, aerosols, organic solvents, wax-based, patches, films, freeze-dried products, etc. according to their dosage forms.

[0018] In some embodiments, in addition to containing the above-mentioned composition, the skin care product can add various ingredients commonly used in the field of skin care products and quasi-drugs according to actual needs without reducing the effect of the composition of the present invention, such as powder ingredients, moisturizers, emulsifiers, light stabilizers, thickeners, solubilizers, metal ion masking agents, pigments, pH adjusters, skin nutrients, vitamins, preservatives, antioxidants, sunscreens, penetration enhancers, liposomes, skin nutrition components, antioxidant adjuvants, flavors, fragrances, pigments, etc., any one or a mixture of several of them.

[0019] Furthermore, a method for preparing a skin care product comprises the following steps:

[0020] (1) preparing the above-mentioned oil control and repairing composition and mixing it evenly;

[0021] (2) Prepare a matrix, add the oil control and repair composition at 45° C. or below, and mix evenly. The matrix is ​​an inactive substance used to prepare skin care products.

[0022] In some embodiments, the matrix includes water, 1,3-propylene glycol, 1,2-hexanediol, glycerin, EDTA-2Na, emulsifiers, emollients, thickeners, soothing and moisturizing ingredients, antioxidants, arginine, and the like.

[0023] In order to verify that the composition has the effect of controlling oil and repairing, pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal pore fungus extract and walnut seed oil were weighed and mixed with a solvent in a certain proportion to form a mixed liquid for experiments, wherein the contents of Hypericum perforatum extract, pyridoxine tri-hexyldecanoate, medicinal pore fungus extract and walnut seed oil were 0.025%, 0.05, 1% and 0.01%, respectively.

[0024] Furthermore, an oil-control and repairing composition is used for preparing an agent for inhibiting PPARγ, wherein the composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal pore fungus extract and Juglans burrardii extract.

[0025] PPARγ is a member of the peroxisome proliferator-activated receptor family, and it is closely related to cell differentiation, proliferation, and lipid metabolism. In the skin, after PPARγ is activated by the ligand, it forms a nuclear transcription factor, which in turn promotes the proliferation and development of sebaceous cells. In this process, lipid droplets are formed and released, causing oil secretion. By inhibiting the expression of PPARγ, PPARγ transcription can be blocked, thereby inhibiting the proliferation and development of sebaceous cells and reducing the formation of lipid droplets, thereby achieving the effect of oil control.

[0026] Furthermore, an oil-control and repairing composition is used for preparing an agent for inhibiting RXRα expression, characterized in that the composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal pore fungus extract and Juglans burrardii extract.

[0027] RXRα, or retinoid X receptor α, plays an important role in the skin, especially in regulating the secretion of oil in the skin. Studies have shown that the expression of RXRα is closely related to the secretion of oil in the skin. Specifically, RXRα affects the secretion of oil in the skin by interacting with HB-EGF (heparin-binding epidermal growth factor); in addition, studies have pointed out that RXRα can also work with PPARγ to form heterodimers or directly regulate C / EBPα to promote cell differentiation, thereby causing oil secretion. The composition of the present invention can inhibit the expression of RXRα to interfere with its interaction with PPARγ, block oil secretion, and thus achieve an oil control effect.

[0028] Furthermore, an oil-control and repairing composition is used for preparing an agent for promoting RARα expression, characterized in that the composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal pore fungus extract and Juglans burrardii extract.

[0029] RARα is sulfonic acid receptor α, one of the receptors of retinoic acid. After binding to the ligand in the skin, it can shorten the mitotic cycle of normal epithelial cells by regulating the expression of molecules such as cAMP, PKC, and TGF-α, affecting the proliferation and differentiation of cells. In sebaceous gland cells, this effect can inhibit the excessive proliferation of sebaceous gland cells, thereby slowing down the production of sebum. The composition of the present invention can promote the expression of RARα, thereby activating more RARα to inhibit the differentiation and proliferation of sebaceous cells, reduce the production of sebum, and achieve the effect of skin oil control.

[0030] Furthermore, an oil-control and repairing composition is used for preparing an agent for inhibiting 5-α reductase, characterized in that the composition includes pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal pore fungus extract and Juglans burrardii extract.

[0031] 5-α reductase is a key enzyme that plays an important role in the metabolism of androgens. It can convert testosterone into dihydrotestosterone (DHT), which is a more potent androgen than testosterone. It can accelerate the proliferation of sebaceous gland cells and sebum synthesis, resulting in oily skin. In addition, the activity of 5-α reductase is directly related to temperature. Increased temperature accelerates the activity of 5-α reductase, thereby increasing the amount of sebum secretion. Specifically, the activity of 5-α reductase increases with increasing temperature, which means that under hotter conditions, 5-α reductase will be more active, promoting the conversion of testosterone to dihydrotestosterone, thereby increasing the secretion activity of sebaceous glands, resulting in increased oil production on the skin. This mechanism not only explains why the skin is more prone to oily in summer or warm environments, but also provides a theoretical basis for controlling sebum secretion by inhibiting the activity of 5-α reductase. The composition of the present invention can inhibit the activity of 5-α reductase, reduce the production of dihydrotestosterone, thereby reducing sebum secretion, and achieve the effect of oil control.

[0032] Furthermore, an oil-control and repairing composition is used for preparing an agent for promoting FLG expression, characterized in that the composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal pore fungus extract and Juglans burrardii extract.

[0033] FLG, or filaggrin, is an important component of the stratum corneum. It is synthesized by keratinocytes and distributed in different parts of the stratum corneum. It is gradually degraded by enzymes during the migration of keratinocytes and degraded into small molecules required by the stratum corneum, such as natural moisturizing factors, and plays an important role in moisturizing and barrier integrity. The precursor of FLG, Pro-FLG, is distributed in the granular layer, while FLG is wrapped outside the keratinocytes in the form of a keratinized membrane and also exists inside the keratinocytes. FLG can be degraded in the stratum corneum of the epidermis to form small amino acid molecules, which have moisturizing functions and are called "natural moisturizing factors". Natural moisturizing factors can maintain the flexibility of the skin by interacting with keratin and keratin fragments. Secondly, the degradation products of FLG are rich in histidine. The trans-uridylic acid (t-UCA) and pyrrolidone-5-carboxylic acid produced after its metabolism can maintain the weakly acidic environment of the skin and resist the invasion of external microorganisms. Trans-uridylic acid (t-UCA) is also the main ultraviolet absorber in the stratum corneum. After being irradiated by ultraviolet rays, t-UCA isomerizes and transforms into cis-urocanic acid cis-UCA, which has an immunosuppressive effect on light. The composition of the present invention can promote basal cells to express FLG to achieve the effect of repairing the skin.

[0034] The present invention has the following beneficial effects:

[0035] (1) The present invention provides an oil-controlling and repairing composition and its application. The composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal porphyra extract and Juglans burrardii extract. The composition achieves oil-control effect by inhibiting PPARγ expression, RXRα expression, 5-α reductase activity and promoting RARα expression of sebaceous gland cells, and achieves repair effect by promoting FLG expression.

[0036] (2) The raw material composition of the composition is simple, with only four ingredients. By utilizing the synergistic effect of the composition, a significant oil control and repair effect is achieved at a lower addition amount. The composition can be used to prepare skin care products, inhibit the secretion of oil by sebaceous glands, and take into account the skin barrier repair at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is a data graph of the PPARγ inhibition rate of the PPARγ expression test of the oil control and repair composition in Example 2;

[0038] Figure 2 This is a data graph of the RXRα inhibition rate of the RXRα expression test of the oil control and repair composition in Example 3;

[0039] Figure 3 This is a data graph of the RARα up-regulation rate of the RARα expression test of the oil-control and repairing composition in Example 4;

[0040] Figure 4 This is a data graph of 5-α reductase inhibition rate of 5-α reductase test of the oil control and repair composition in Example 5;

[0041] Figure 5 This is a data graph of the FLG content increase rate of the oil control and repair composition of Example 6 in the test of increasing the FLG content. DETAILED DESCRIPTION

[0042] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be pointed out that the embodiments described below are intended to facilitate the understanding of the present invention and do not have any limiting effect on the present invention.

[0043] Example 1: Preparation of Oil Control and Repairing Composition Reagents

[0044] Pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal vesiculosus extract and walnut seed oil are weighed and prepared with a solvent in a certain proportion to form a mixed solution, wherein the contents of Hypericum perforatum extract, pyridoxine tri-hexyldecanoate, medicinal vesiculosus extract and walnut seed oil are 0.025%, 0.05%, 1% and 0.01%, respectively.

[0045] Example 2: PPARγ expression test of oil control and repair composition

[0046] The composition in Example 1 was used for gene expression testing, and a blank group, a positive control group and a sample group were set, wherein the positive control was isotretinoin, samples 1-4 were individual components of the composition, samples 5-8 were any three of the components in the composition, and sample 9 was the combination. The expression of PPARγ in each group was tested using a sebaceous gland cell model. The results are shown in Table 1 and Attached Figure 1 shown.

[0047] Table 1. PPARγ expression test

[0048]

[0049] According to Table 1 and Appendix Figure 1 Analysis shows that samples 1-4 are individual components of the composition, and the individual components have an inhibitory effect on PPARγ expression. The inhibition rate of sample 1 medicinal pore fungus extract is 40%, and the inhibition rate of sample 2 pyridoxine tri-hexyldecanoate is 44%. Samples 1 and 2 have a significant inhibitory effect on PPARγ expression compared with sample 3 Hypericum perforatum extract and sample 4 Walnut seed oil. The inhibition rate of sample 4 Walnut seed oil on PPARγ expression is only 1%, and the inhibitory effect is very small. Samples 5-8 are any three of the compositions, and sample 9 is a composition. These five groups of experimental samples are to verify that the components of the composition have a synergistic effect to inhibit PPARγ expression. The experimental results show that the inhibition rate of the three-component composition of samples 5-8 is significantly higher than the inhibition rate of the individual components of samples 1-4, but they are all less than the inhibition rate of the four-component composition of sample 9, which is 61%, indicating that the composition of sample 9 has a more significant effect on inhibiting PPARγ expression.

[0050] In the skin, after PPARγ is activated by ligands, it forms nuclear transcription factors, which in turn promote the proliferation and development of sebaceous cells. In this process, lipid droplets are formed and released, causing oil secretion. The composition of the present invention can inhibit the expression of PPARγ, block PPARγ transcription, and then inhibit the proliferation and development of sebaceous cells, reduce the formation of lipid droplets, and thus achieve the effect of controlling oil.

[0051] Example 3: RXRα expression test of oil control and repair composition

[0052] The composition in Example 1 was used for gene expression testing, and a blank group, a positive control group and a sample group were set, wherein the positive control was isotretinoin, samples 1-4 were individual components of the composition, samples 5-8 were any three of the components in the composition, and sample 9 was the combination. The expression of RXRα in each group was tested using a sebaceous gland cell model, and the results are shown in Table 2 and Attached Figure 2 shown.

[0053] Table 2. RXRα expression test

[0054]

[0055] According to Table 2 and Appendix Figure 2 Analysis shows that samples 1-4 are individual components of the composition. The individual components sample 1, medicinal pore fungus extract, sample 2, pyridoxine tri-hexyldecanoate, and sample 3, Hypericum perforatum extract, all have inhibitory effects on RXRα expression, while the inhibition rate of sample 4, walnut seed oil, on RXRα expression is 1%, indicating that walnut seed oil has basically no inhibitory effect on RXRα expression. Samples 5-8 are any three of the compositions, and sample 9 is a composition. These five groups of experimental samples are to verify that the components of the composition have a synergistic effect to inhibit RXRα expression. The experimental results show that the inhibition rate of the three-component composition of samples 5-8 is significantly higher than that of the individual components of samples 1-4, but is significantly lower than the inhibition rate of the four-component composition of sample 9, which is 48%, indicating that the composition of sample 9 has a more significant effect on inhibiting RXRα expression.

[0056] RXRα, or retinoid X receptor α, plays an important role in the skin, especially in regulating the secretion of skin oil. Studies have shown that the expression of RXRα is closely related to the secretion of skin oil. Specifically, RXRα affects the secretion of skin oil by interacting with HB-EGF (heparin-binding epidermal growth factor); in addition, studies have pointed out that RXRα can also work with PPARγ to form heterodimers or directly regulate C / EBPα to promote cell differentiation, thereby causing oil secretion. The composition of the present invention can inhibit the expression of RXRα, thereby interfering with the interaction between RXRα and heparin-binding epidermal growth factor, and interfering with its synergistic effect with PPARγ, blocking the differentiation of sebaceous cells, thereby achieving an oil control effect.

[0057] Example 4: RARα expression test of oil control and repair composition

[0058] The composition in Example 1 was used for gene expression testing, and a blank group, a positive control group and a sample group were set, wherein the positive control was isotretinoin, samples 1-4 were individual components of the composition, samples 5-8 were any three of the components in the composition, and sample 9 was the combination. The expression of RARα in each group was tested using a sebaceous gland cell model, and the results are shown in Table 3 and Attached Figure 3 shown.

[0059] Table 3. RARα expression test

[0060]

[0061] According to Table 3 and Appendix Figure 3Analysis shows that sample 1 medicinal pore fungus extract, sample 2 pyridoxine tri-hexyldecanoate and sample 4 walnut seed oil have almost no effect on increasing RARα expression, with an increase rate of 3%, 3% and 2% respectively, while only sample 3 Hypericum perforatum extract has a significant effect on increasing RARα expression, with an increase rate of 27%. Samples 5-8 are any three of the compositions, and sample 9 is a composition. These five groups of experimental samples are to verify that the components of the composition have a synergistic effect to increase RARα expression, because samples 1, 2 and 4 have almost no effect on RARα expression, so the synergistic effect of the combination of sample 6 medicinal pore fungus extract + pyridoxine tri-hexyldecanoate + walnut seed oil on RARα expression is very limited, only 1%; on the contrary, samples 5, 7, 8 and 9 all have an increase in RARα expression, but the increase rates of samples 5, 7 and 8 are all lower than those of sample 9, indicating that the sample 9 composition is more significant in increasing RARα expression. At the same time, it was found that even if the medicinal porphyra extract, pyridoxine tri-hexyldecanoate, and walnut seed oil were used alone, the effect of enhancing the expression of RARα was very limited, but as long as one or more of these three were used together with Hypericum perforatum extract, the expression effect of RARα could be significantly enhanced, proving that there is a significant synergistic effect between Hypericum perforatum extract and the other three components.

[0062] RARα is sulfonic acid receptor α, one of the receptors of retinoic acid. After binding to the ligand in the skin, it can shorten the mitotic cycle of normal epithelial cells by regulating the expression of molecules such as cAMP, PKC, and TGF-α, affecting the proliferation and differentiation of cells. In sebaceous gland cells, this effect can inhibit the excessive proliferation of sebaceous gland cells, thereby slowing down the production of sebum. The composition of the present invention can promote the expression of RARα, thereby activating more RARα to inhibit the differentiation and proliferation of sebaceous cells, reduce the production of sebum, and achieve the effect of skin oil control.

[0063] Example 5: 5-α-reductase test of oil control and repair composition

[0064] The composition in Example 1 was used for gene expression test, and a blank group, a positive control group and a sample group were set, wherein the positive control was isotretinoin, samples 1-4 were individual components of the composition, samples 5-9 were combinations of two or any three components in the composition, and sample 10 was the combination. The inhibition rate of 5-α reductase was calculated and tested, and the results are shown in Table 4 and Attached Figure 4 shown.

[0065] Table 4. 5-α-reductase test

[0066]

[0067]

[0068] According to Table 4 and Appendix Figure 4 Analysis shows that samples 1-4 are individual components of the composition. Individual components of samples 1-4 have an inhibitory effect on 5-α reductase activity. The inhibition rate of sample 1, medicinal pore fungus extract, on 5-α reductase activity is 27.20%, which is more significant. The inhibition rate of sample 4, walnut seed oil, on 5-α reductase activity is only 0.43%, indicating that walnut seed oil has a small effect on inhibiting 5-α reductase activity. Samples 5-9 are a combination of two or any three components in the composition, and sample 10 is a composition. These six groups of experimental samples are to verify that the components of the composition have a synergistic effect to inhibit 5-α reductase activity. The experimental results show that the inhibition rate of the combination of components of samples 5-9 is significantly higher than that of the individual components of samples 1-4, but is less than the inhibition rate of the four-component composition of sample 10, which is 45.63%, indicating that the composition of sample 10 has a more significant effect on inhibiting 5-α reductase activity.

[0069] 5-α reductase is a key enzyme that plays an important role in the metabolism of androgens. It can convert testosterone into dihydrotestosterone (DHT), which is a more potent androgen than testosterone and can accelerate the proliferation of sebaceous gland cells and sebum synthesis, thereby causing oily skin. The composition of the present invention can inhibit the activity of 5-α reductase, thereby reducing the conversion of testosterone into dihydrotestosterone by 5-α reductase, thereby slowing down the proliferation of sebaceous gland cells and the rate of sebum synthesis, reducing the secretion of oil, and achieving the effect of oil control.

[0070] Example 6: Test on the oil control and repair composition for increasing FLG content

[0071] The composition of Example 1 was used for FLG content test, and a blank group, a negative control group, a positive control group and a sample group were set. The positive control raw material was WY14643, samples 1-4 were individual components of the composition, samples 5-9 were combinations of two or any three components in the composition, and sample 10 was a combination. The negative control group, the positive control group and the sample group were heated at 600 mJ / cm 2 The blank group was not stimulated by 600 mJ / cm2 UVB light, and the IOD value increase rate was calculated. The results are shown in Table 5 and Attached Figure 5 shown.

[0072] Table 5. FLG content test

[0073]

[0074] According to Table 5 and Appendix Figure 5Analysis shows that samples 1-4 are individual components of the composition. Compared with the blank control, the individual component samples, sample 1, medicinal porphyra extract, sample 2, pyridoxine tri-hexyldecanoate, and sample 4, walnut seed oil, have no significant effect on improving FLG expression. Only sample 3, Hypericum perforatum extract, has a significant improvement effect on FLG expression increase rate of 184.85%. Samples 5-9 are two or any three components of the composition, and sample 10 is a combination. These six groups of experimental samples are to verify that the components of the composition have a synergistic effect to improve the expression of FLG. The experimental results show that the combination of components of samples 5-9 significantly improves FLG expression compared to the individual components of samples 1-4, but they are all less than the increase rate of 325.15% of the four-component combination of sample 10 on improving FLG expression, indicating that the composition of sample 10 has a more significant effect on improving FLG expression. At the same time, it was found that even if the use of medicinal porphyra extract, pyridoxine tri-hexyldecanoate, and walnut seed oil alone had no significant effect on increasing FLG expression, as long as one or more of these three were used together with Hypericum perforatum extract, the effect of FLG expression could be greatly improved, proving that Hypericum perforatum extract and the other three components had a significant synergistic effect.

[0075] FLG is an important component of the stratum corneum, synthesized by keratinocytes, distributed in different parts of the stratum corneum, and gradually degraded by enzymes during the migration of keratinocytes, degraded into small molecules required by the stratum corneum such as natural moisturizing factors, and plays an important role in moisturizing and barrier integrity. The composition of the present invention can promote basal cells to express more FLG. On the one hand, FLG can be degraded in the epidermal stratum corneum to form amino acid small molecules, i.e., natural moisturizing factors, which have moisturizing functions. They can maintain the flexibility of the skin through interaction with keratin and keratin fragments. On the other hand, the degradation products of FLG are rich in histidine, and the trans-uridylic acid (t-UCA) and pyrrolidone-5-carboxylic acid produced after they are metabolized can maintain the weakly acidic environment of the skin and resist the invasion of external microorganisms; trans-uridylic acid (t-UCA) is also the main ultraviolet absorber in the stratum corneum. After ultraviolet irradiation, t-UCA isomerizes and transforms into cis-urocanic acid cis-UCA, which has an immunosuppressive effect on light.

[0076] In summary, the composition of the present invention promotes keratinocytes to produce more FLG, and plays a role in protecting and repairing the skin through the above-mentioned pathways.

[0077] Example 7: Preparation of an oil-control repair lotion with an added oil-control repair composition

[0078] Premix the raw materials water, propylene glycol, glycerin, EDTA-2Na, etc., stir evenly, and then stir the thickener until it is completely dissolved as the water phase; premix and stir the emollients such as white meadowfoam seed oil and polydimethylsiloxane with hexylene glycol as the oil phase; add the water phase to the main pot, heat to 50-60°C, add the emulsifier and the oil phase, homogenize and emulsify, and then cool down. After the main pot is cooled to 45°C, add the soothing and moisturizing ingredients and the oil control and repair composition, and finally add the antioxidant, test the pH, and if necessary, add arginine to adjust the pH to 6-7. The mass ratio of pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal lamella extract and walnut extract in the composition is (0.005-0.5): (0.001-1): (0.1-5): (0.001-0.5), and the specific raw material addition amount range is shown in Table 7 below.

[0079] Table 7. Raw materials and addition amount range

[0080]

[0081]

[0082] Although the present invention is disclosed as above, the present invention is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the scope defined by the claims.

Claims

1. An oil control and repairing composition, It is characterized in that Including pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal porphyra extract and Burgundy walnut extract.

2. The oil control and repairing composition according to claim 1, It is characterized in that The Hypericum perforatum extract includes one or more of Hypericum perforatum flower extract, Hypericum perforatum twig extract, Hypericum perforatum leaf extract, Hypericum perforatum stem extract, and Hypericum perforatum oil; the Juglans walnut extract includes one or more of Juglans walnut seed oil and Juglans walnut fruit extract.

3. The oil control and repairing composition according to claim 1, It is characterized in that The mass ratio of pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal porphyra extract and Juglans burrardii extract is (0.005-0.5): (0.001-1): (0.1-5)：(0.001-0.5)。 4. A skin care product, It is characterized in that Contains the oil control repair composition according to any one of claims 1 to 3.

5. A method for preparing a skin care product, It is characterized in that The following steps are involved: (1) preparing the oil control and repairing composition according to any one of claims 1 to 4, and mixing the mixture; (2) Prepare a matrix, add the oil control and repair composition at 45° C. or below, and mix evenly. The matrix is ​​an inactive substance used to prepare skin care products.

6. Use of an oil control and repairing composition for preparing an agent for inhibiting PPARγ expression, It is characterized in that The composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal porphyra extract and Juglans burrardii extract.

7. Use of an oil control and repairing composition for preparing an agent for inhibiting RXRα expression, It is characterized in that The composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal porphyra extract and Juglans burrardii extract.

8. Use of an oil control and repairing composition for preparing an agent for promoting RARα expression, It is characterized in that The composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal porphyra extract and Juglans burrardii extract.

9. Use of an oil control and repairing composition for preparing an agent for inhibiting 5-α reductase activity, It is characterized in that The composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal porphyra extract and Juglans burrardii extract.

10. Use of an oil control and repairing composition for preparing a reagent for promoting FLG expression, It is characterized in that The composition comprises pyridoxine tri-hexyldecanoate, Hypericum perforatum extract, medicinal porphyra extract and Juglans burrardii extract.

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