Primer probe detection kit for digestive tract tumor marker and detection method thereof

Abstract

The invention relates to the technical field of probe detection, in particular to a primer probe detection kit for digestive tract tumor markers and a detection method thereof.The primer probe detection kit comprises a detection box and a storage groove formed in the detection box, a hollow rod is installed in the detection box, a first through groove is formed in the circumferential outer wall of the hollow rod, and a fixing disc is arranged at the end of the hollow rod; the pushing assembly is arranged in the storage groove, and a pushing plate is connected to the pushing assembly; the rotary pushing mechanism is arranged on the fixed disc, a clamping regulation and control assembly is arranged on the rotary pushing mechanism, and clamping plates which are symmetrically arranged are connected to the clamping regulation and control assembly; the pressing and rotating mechanism is arranged in the detection box and connected with the rotary pushing mechanism, a trigger follow-up mechanism connected with the pressing and rotating mechanism is further arranged in the detection box, and a second inclined plate and a second limiting wheel are connected to the trigger follow-up mechanism. And the detection reagent can be stored.

Description

Technical Field

[0001] The present invention relates to the field of probe detection technology, in particular to a primer probe detection kit for digestive tract tumor markers and a detection method thereof. Background Art

[0002] Digestive tract tumor markers are indicators for determining whether a patient has a digestive tract tumor, mainly including carcinoembryonic antigen, alpha-fetoprotein, carbohydrate antigen 199, etc.

[0003] Gastrointestinal tumor markers can usually be checked by blood tests for tumor markers, occult blood tests, etc. Blood tumor marker testing is a common method of examination, and the main screening indicators for gastrointestinal tumors are 4 to 5 items.

[0004] When testing is performed using a primer probe test kit, it is usually necessary to manually open the kit and take out the test reagents. After the test is completed, the test reagents are placed back into the kit. However, this may cause confusion about the test reagents, and the inconvenience of taking the test reagents may lead to inconvenience in use. Summary of the invention

[0005] The object of the present invention is to provide a primer-probe detection kit for digestive tract tumor markers and a detection method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions: Primer probe detection kit for digestive tract tumor markers, including: A detection box, and a storage slot provided in the detection box, a hollow rod is installed in the detection box, a first through slot is provided on the circumferential outer wall of the hollow rod, and a fixing plate is provided at the end of the hollow rod; Also includes: A pushing component is arranged in the storage tank, and a pushing plate is connected to the pushing component; A rotating pushing mechanism is arranged on the fixed plate, and a clamping and regulating component is arranged on the rotating pushing mechanism, and a symmetrically arranged clamping plate is connected to the clamping and regulating component, and the clamping and regulating component can adjust the distance between the two clamping plates when the rotating pushing mechanism moves; A pressing and rotating mechanism is arranged in the detection box and connected to the rotating pushing mechanism. A trigger following mechanism connected to the pressing and rotating mechanism is also arranged in the detection box. A second inclined plate and a second limiting wheel are connected to the trigger following mechanism. The pressing and rotating mechanism can drive the rotating pushing mechanism to move. The pressing and rotating mechanism can also drive the second inclined plate and the second limiting wheel to move toward or away from each other through the trigger following mechanism.

[0007] As a further solution of the present invention: the pushing assembly includes a fixed sleeve installed in the storage groove and symmetrically arranged, a movable rod is slidably installed in the fixed sleeve, the movable rod is connected to the push plate, and a first spring abutting against the push plate is sleeved on the fixed sleeve and the movable rod.

[0008] As a further solution of the present invention: the rotating pushing mechanism includes a rotating sleeve rotatably mounted on the fixed disk, the rotating sleeve is provided with a rotating disk, the clamping plate is rotatably connected to the rotating disk, the rotating disk is provided with a plurality of sliding grooves equidistantly distributed in a circle, a receiving ring is provided in the sliding groove, and the fixed disk is provided with a guide assembly connected to the sliding groove.

[0009] As a further solution of the present invention: the guide assembly includes an annular groove and an oblique groove opened on the fixed plate, a sliding block is slidably installed in the sliding groove, a limiting column is arranged on the side wall of the sliding block, and the limiting column is slidably connected to the annular groove and the oblique groove.

[0010] As a further solution of the present invention: the clamping and regulating assembly includes a supporting sleeve installed on the sliding block, a slot is provided on the outer wall of the supporting sleeve, a supporting rod is slidably installed in the supporting sleeve, a first limiting block slidably connected to the slot is provided on the supporting rod, a second spring is sleeved on the supporting sleeve and the supporting rod, a symmetrically arranged connecting rod is hinged at the end of the supporting rod, and the connecting rod is hinged to the clamping plate.

[0011] As a further solution of the present invention: the pressing and rotating mechanism includes a guide rod slidably mounted on the detection box, a pressure plate is provided at the end of the guide rod, a push ring is provided on the guide rod, the push ring is slidably connected to the rotating sleeve, and a driven component connected to the push ring is provided on the rotating sleeve.

[0012] As a further solution of the present invention: the driven component includes a guide groove opened on the outer wall of the rotating sleeve, the inner wall of the pushing ring is provided with a second limit block slidably connected to the guide groove, and the rotating sleeve is sleeved with a third spring abutting against the pushing ring.

[0013] As a further solution of the present invention: the trigger follower mechanism includes a second through groove opened on the outer wall of the rotating sleeve, a limit ring abutting the push ring is slidably installed in the second through groove, a limit rod is rotatably installed on the limit ring, a first inclined plate slidably connected to the first through groove is arranged at the end of the limit rod, and an elastic component connected to the first inclined plate is arranged in the detection box.

[0014] As a further solution of the present invention: the elastic component includes a guide sleeve installed in the detection box and symmetrically arranged, a receiving rod is slidably installed in the guide sleeve, a first limiting wheel is arranged at one end of the receiving rod, and the other end of the receiving rod is respectively connected to the second inclined plate and the second limiting wheel, the first limiting wheel abuts against the first inclined plate, and the receiving rod is sleeved with a fourth spring abutting against the guide sleeve.

[0015] The detection method of the primer-probe detection kit for digestive tract tumor markers comprises the following steps: Step 1: Place multiple test reagents in the storage tank, and under the action of the push component, control one of the test reagents to be located between the two card plates through the push plate; Step 2: At this time, the pressing and rotating mechanism can be controlled by human power to move, thereby driving the rotating and pushing mechanism to move. Under the action of the rotating and pushing mechanism, the two card plates are controlled by the clamping and regulating component to move toward each other to fix the detection reagent and rotate around the central axis of the fixed disk; Step 3: When the card plate moves to the desired position, the two card plates move away from each other under the action of the clamping and regulating assembly, and the trigger follower mechanism moves under the action of the pressing and rotating mechanism to control the second inclined plate and the second limiting wheel to move away from each other. The second inclined plate will control the detection reagent to move out of the detection box to facilitate the user to take it; Step 4: When the test is completed, continue to press the rotating mechanism to move, and place the test reagent between the two card plates again. When the test reagent moves to the position that cooperates with the second limiting wheel, under the action of the second limiting wheel, the test reagent is pushed out of the test box, and the above steps are repeated to achieve the purpose of automatically storing and retrieving the test reagent.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: the present application can automatically control the ejection or storage of the detection reagent by manually controlling the pressing and rotating mechanism, so as to facilitate the use of the detection reagent. Under the action of the pushing component, one of the detection reagents is located between the two card plates. When the pressing and rotating mechanism is driven by human power to move, the rotating pushing mechanism is driven to move, so as to control the two card plates to move toward each other through the clamping and regulating component. At the same time, the card plate will rotate around the central axis of the fixed disk to control the movement of the detection reagent to the delivery position. The pressing and rotating mechanism will also drive the trigger follower mechanism to move, and under the action of the second inclined plate and the second limiting wheel, the removal or storage of the detection reagent is controlled to facilitate the use of the detection reagent. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The figure is a schematic diagram of the structure of an embodiment of a primer-probe detection kit for digestive tract tumor markers.

[0018] Figure 2 The figure is a schematic diagram of the cross-sectional structure of the detection box in the embodiment of the primer-probe detection kit for digestive tract tumor markers.

[0019] Figure 3 This is a schematic diagram of the internal structure of the detection box in the embodiment of the primer-probe detection kit for digestive tract tumor markers.

[0020] Figure 4 The figure is a schematic diagram of the connection relationship between the pressing and rotating mechanism, the rotating and pushing mechanism, and the clamping and regulating component in the embodiment of the primer and probe detection kit for digestive tract tumor markers.

[0021] Figure 5 for Figure 4 A schematic diagram of the enlarged structure at point A in the middle.

[0022] Figure 6 The diagram is a structural diagram of a part of the rotating pushing mechanism and a part of the triggering following mechanism in an embodiment of a primer probe detection kit for digestive tract tumor markers.

[0023] Figure 7 It is a schematic diagram of the exploded structure of part of the rotating pushing mechanism and the clamping and regulating component in the embodiment of the primer probe detection kit for digestive tract tumor markers.

[0024] Figure 8 This is a schematic diagram of the explosion structure of a part of the pressing and rotating mechanism in the embodiment of the primer probe detection kit for digestive tract tumor markers.

[0025] Fig. 9 for Figure 8 A magnified schematic diagram of the structure at point B in the middle.

[0026] Fig.10 The schematic diagram of the structure of the primer probe detection kit for digestive tract tumor markers is a partial pressing and rotating mechanism and a partial triggering and following mechanism in the embodiment.

[0027] In the figure: 1, detection box; 101, storage slot; 2, fixed sleeve; 3, movable rod; 4, first spring; 5, push plate; 6, hollow rod; 601, first through slot; 7, fixed plate; 701, annular slot; 702, inclined slot; 8, rotating sleeve; 801, first vertical slot; 802, first spiral slot; 803, second vertical slot; 804, second spiral slot; 805, second through slot; 9, rotating plate; 901, slide slot; 10, receiving ring; 11, sliding block; 12, supporting sleeve ; 1201, slot; 13, support rod; 1301, first limit block; 14, second spring; 15, connecting rod; 16, card plate; 17, pressure plate; 18, guide rod; 19, push ring; 1901, second limit block; 20, third spring; 21, limit ring; 22, limit rod; 23, first inclined plate; 24, limit column; 25, guide sleeve; 26, receiving rod; 27, fourth spring; 28, first limit wheel; 29, second inclined plate; 30, second limit wheel. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] In addition, when an element in the present invention is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only embodiment.

[0030] See also Figure 1 to Figure 10 In an embodiment of the present invention, a primer-probe detection kit for digestive tract tumor markers comprises: A detection box 1, and a storage slot 101 provided in the detection box 1, a hollow rod 6 is installed in the detection box 1, a first through slot 601 is provided on the outer circumferential wall of the hollow rod 6, and a fixing plate 7 is provided at the end of the hollow rod 6; Also includes: See also Figure 2 , Figure 3, a pushing component is arranged in the storage groove 101, and a pushing plate 5 is connected to the pushing component. The pushing component includes a fixed sleeve 2 installed in the storage groove 101 and symmetrically arranged, and a movable rod 3 is slidably installed in the fixed sleeve 2, and the movable rod 3 is connected to the pushing plate 5. A first spring 4 that abuts against the pushing plate 5 is sleeved on the fixed sleeve 2 and the movable rod 3.

[0031] In detail, when testing is required, multiple testing reagents can be placed in the storage groove 101, and the testing reagents will abut against the push plate 5, so that the movable rod 3 is located at the end of the stroke toward the fixed sleeve 2, so that the first spring 4 is in a compressed state. Under the action of the push plate 5, one of the testing reagents located away from the push plate 5 is located between the two card plates 16. Under the action of the first spring 4, the automatic loading of the testing reagents can be ensured.

[0032] See also Figure 2-Figure 4 , Figure 6 , Figure 7 , a rotating pushing mechanism is arranged on the fixed disk 7, the rotating pushing mechanism includes a rotating sleeve 8 rotatably mounted on the fixed disk 7, a rotating disk 9 is arranged on the rotating sleeve 8, the clamping plate 16 is rotatably connected to the rotating disk 9, a plurality of slide grooves 901 equidistantly distributed in a circle are provided on the rotating disk 9, a receiving ring 10 is arranged in the slide groove 901, a guiding assembly connected to the slide groove 901 is arranged on the fixed disk 7, wherein the guiding assembly includes an annular groove 701 and an inclined groove 702 arranged on the fixed disk 7, a sliding block 11 is slidably installed in the slide groove 901, a limiting column 24 is arranged on the side wall of the sliding block 11, and the limiting column 24 is slidably connected to the annular groove 701 and the inclined groove 702.

[0033] It should be noted that two conveying holes are provided on the detection box 1, and the inclined grooves 702 are symmetrically arranged, and one end of the two inclined grooves 702 is connected to each other, and the other end is connected to the end of the annular groove 701. In the initial state, the limit column 24 is located at the connection position of the two inclined grooves 702, so that the sliding block 11 is located at the end of the stroke on one side of the sliding groove 901. Therefore, the spacing between the sliding blocks 11 is the smallest. Under the action of the sliding blocks 11, the angle between the two card plates 16 is controlled to be the largest through the clamping and regulating component. The push plate 5 can control one of the detection reagents to enter between the two card plates 16 and abut against the receiving ring 10. When the rotating sleeve 8 rotates, it drives the rotating disk 9 to move, thereby driving the sliding block 11 to move through the sliding groove 901, so as to control the card plate 16 through the clamping and regulating component. Movement, at the same time, the sliding block 11 will also drive the limiting column 24 to slide along the inclined groove 702, so that the sliding block 11 moves in the direction away from each other, when the limiting column 24 moves into the annular groove 701, the angle between the card plates 16 is the smallest, so as to fix the detection reagent in the receiving ring 10, when the card plate 16 holding the detection reagent moves to the position cooperating with one of the conveying holes, the limiting column 24 moves between the two inclined grooves 702 again, so that the angle between the two card plates 16 returns to the initial state, at this time, the detection reagent can move freely, and the other two card plates 16 move to the position cooperating with the storage groove 101, under the action of the push plate 5, the new detection reagent is transported between the two card plates 16 again, and the above steps are repeated to realize automatic loading of the detection reagent.

[0034] See also Figure 2-Figure 4 , Figure 7 The rotary pushing mechanism is provided with a clamping and regulating component, and a symmetrically arranged card plate 16 is connected to the clamping and regulating component. The clamping and regulating component can adjust the distance between the two card plates 16 when the rotary pushing mechanism moves. The clamping and regulating component includes a support sleeve 12 installed on the sliding block 11, and a card groove 1201 is opened on the outer wall of the support sleeve 12. A support rod 13 is slidably installed in the support sleeve 12, and a first limit block 1301 slidably connected to the card groove 1201 is provided on the support rod 13. A second spring 14 is sleeved on the support sleeve 12 and the support rod 13. A symmetrically arranged connecting rod 15 is hinged at the end of the support rod 13, and the connecting rod 15 is hinged to the card plate 16.

[0035] Further, in the initial state, the sliding block 11 is located at the end of the stroke on one side of the slide groove 901, and the distance between the sliding blocks 11 is the smallest, and the second spring 14 is in a compressed state, so that the support rod 13 is located at the end of the stroke outside the support sleeve 12, and the first limit block 1301 is located at the end of the stroke on one side of the card slot 1201. At this time, the distance between the support rod 13 and the card plate 16 is the largest. Under the action of the connecting rod 15, the angle between the card plates 16 is maximized, ensuring that the detection reagent can freely enter between the card plates 16. When the rotating disk 9 rotates, it drives the sliding block 11 to move, and the limit column 24 and the inclined groove 7 02 and the annular groove 701, the sliding block 11 moves in the direction away from each other, and the sliding block 11 also drives the support sleeve 12 to move, and drives the support rod 13 to move through the second spring 14, so as to control the two clamping plates 16 to rotate in the direction of approaching each other through the connecting rod 15, until the clamping plate 16 moves to a position in which the detection reagent is fitted, thereby fixing the detection reagent through the clamping plate 16 and the receiving ring 10, and the sliding block 11 continues to move, so that the size of the support sleeve 12 and the support rod 13 is increased, so that the compression amount of the second spring 14 is increased, so as to increase the clamping force on the detection reagent.

[0036] Preferably, as the rotating disk 9 rotates, when the rotating disk 9 rotates a quarter of a turn, the limit column 24 will move to the next inclined groove 702, so that the distance between the sliding blocks 11 is in the minimum state again. Under the action of the connecting rod 15, the opening angle of the card plate 16 is maximized again. At this time, the detection reagent can be taken out freely.

[0037] See also Figure 1-Figure 5 , Figure 8-Figure 10 , a pressing and rotating mechanism is arranged in the detection box 1 and connected to the rotating pushing mechanism, the pressing and rotating mechanism includes a guide rod 18 slidably mounted on the detection box 1, a pressure plate 17 is arranged at the end of the guide rod 18, a pushing ring 19 is arranged on the guide rod 18, the pushing ring 19 is slidably connected to the rotating sleeve 8, and a driven component connected to the pushing ring 19 is arranged on the rotating sleeve 8, wherein the driven component includes a guide groove opened on the outer wall of the rotating sleeve 8, a second limit block 1901 slidably connected to the guide groove is arranged on the inner wall of the pushing ring 19, and a third spring 20 abutting against the pushing ring 19 is sleeved on the rotating sleeve 8.

[0038] Furthermore, the guide groove can be divided into multiple sections and has four groups equidistantly distributed around the circumference, namely the first vertical groove 801, the first spiral groove 802, the second vertical groove 803, and the second spiral groove 804, and the first vertical groove 801, the first spiral groove 802, the second vertical groove 803, and the second spiral groove 804 are connected to each other in sequence. In the initial state, the third spring 20 is in a compressed state, so that the second limit block 1901 is located at the end of the stroke of the first vertical groove 801 away from the rotating disk 9. When the detection reagent needs to be used, the pressure plate 17 can be manually pressed at this time, and the guide rod 18 can be driven to move, thereby driving the push ring 19 to move. The push ring 19 will also drive the second limit block 1901 to move along the length direction of the first vertical groove 801. When the second limit block 1901 is separated from the first vertical groove 801 and enters the first spiral groove 802, the rotating sleeve 8 will rotate, so that The card plate 16 rotates around the rotating sleeve 8. When the second limit block 1901 moves to the connecting position of the first spiral groove 802 and the second vertical groove 803, the rotating sleeve 8 rotates one-eighth of a circle, and the compression of the third spring 20 reaches the maximum. At this time, the force applied to the pressure plate 17 is removed, the third spring 20 is elastically released, and drives the push ring 19 to reset. The push ring 19 will also drive the second limit block 1901 to move along the length direction of the second vertical groove 803. When the second limit block 1901 enters the second spiral groove 804, the rotating sleeve 8 rotates again. When the second limit block 1901 disengages from the second spiral groove 804 and enters the next first vertical groove 801, the rotating sleeve 8 just rotates one-eighth of a circle again, and the card plate 16 holding the detection reagent moves to a position cooperating with one of the delivery holes. Repeat the above steps to realize automatic delivery of the detection reagent.

[0039] See also Figure 2 , Figure 3 , Figure 6 , Fig.10, the detection box 1 is also provided with a trigger follower mechanism connected to the pressing and rotating mechanism, and the trigger follower mechanism is connected to a second inclined plate 29 and a second limiting wheel 30, and the pressing and rotating mechanism can drive the rotation pushing mechanism to move, and the pressing and rotating mechanism can also drive the second inclined plate 29 and the second limiting wheel 30 to move toward or away from each other through the trigger follower mechanism, and the trigger follower mechanism includes a second through groove 805 provided on the outer wall of the rotating sleeve 8, and a limiting ring 21 abutting against the pushing ring 19 is slidably installed in the second through groove 805, and a limiting rod 22 is rotatably installed on the limiting ring 21, and the A first inclined plate 23 slidably connected to the first through groove 601 is provided at the end of the limiting rod 22, and an elastic component connected to the first inclined plate 23 is provided in the detection box 1, wherein the elastic component includes a guide sleeve 25 installed in the detection box 1 and symmetrically arranged, and a receiving rod 26 is slidably installed in the guide sleeve 25, and a first limiting wheel 28 is provided at one end of the receiving rod 26, and the other end of the receiving rod 26 is respectively connected to the second inclined plate 29 and the second limiting wheel 30, the first limiting wheel 28 abuts against the first inclined plate 23, and a fourth spring 27 abuts against the guide sleeve 25 is sleeved on the receiving rod 26.

[0040] To elaborate, in the initial state, under the action of the third spring 20, the push ring 19 is located at the end of the stroke away from the rotating disk 9, so that the limit rod 22 is controlled to be located at the end of the stroke away from the bottom of the detection box 1 through the limit ring 21, and the first inclined plate 23 will control the distance between the second inclined plate 29 and the second limit wheel 30 to be maximum through the first limit wheel 28. When the pressure plate 17 moves toward the direction of the detection box 1, the push ring 19 is driven to move toward the rotating disk 9, so that the push ring 19 is separated from the limit ring 21, and the fourth spring 27 is elastically released and drives the two first limit wheels 28 to move in a direction close to each other. The first limit wheel 28 fits with the inclined surface of the first inclined plate 23 to control the movement of the limit rod 22 through the first inclined plate 23, so that the limit The ring 21 moves to the end of the stroke on one side of the second through groove 805. When the push ring 19 moves again in the direction away from the rotating disk 9 and abuts against the limit ring 21, the limit column 24 is located between the two inclined grooves 702, so as to control the detection reagent to be located above the second inclined plate 29 through the clamping plate 16. The limit ring 21 will also drive the limit rod 22 to move, and under the action of the inclined surface of the first inclined plate 23, control the two first limit wheels 28 to move in the direction away from each other, so as to control the second inclined plate 29 and the second limit wheel 30 to move in the direction away from each other through the receiving rod 26. When the inclined surface of the second inclined plate 29 fits with the detection reagent, it drives the detection reagent to move in the direction away from the bottom of the detection box 1 and pass through the delivery hole to facilitate the user to use the detection reagent.

[0041] Preferably, a discharge slot is provided on the side wall of the detection box 1, and a storage box cooperating with the discharge slot can be installed on the side wall of the detection box 1. When the detection reagent is used up, the rotating disk 9 can be controlled to rotate again, and the two card plates 16 located at the cooperating position of the delivery hole can be controlled to move to another cooperating position of the delivery hole. At this time, the detection reagent can be inserted between the two card plates 16 again through the delivery hole. Since the second inclined plate 29 is not provided at this position, the detection reagent can completely enter the detection box 1. When the rotating disk 9 rotates again and drives the two card plates 16 to move toward the cooperating position with the second limiting wheel 30, the detection reagent is stored in the detection box 1. Before the column 24 moves to the first vertical groove 801, the distance between the second limiting wheel 30 and the fixed sleeve 2 is the smallest. When the limiting column 24 disengages from the second spiral groove 804 and enters the first vertical groove 801, the two clamping plates 16 just move to the matching position with the second limiting wheel 30. At this time, under the action of the pushing ring 19, the limiting rod 22 moves to control the second limiting wheel 30 to move away from the fixed sleeve 2 through the first inclined plate 23. Under the action of the second limiting wheel 30, the used detection reagent is pushed out of the detection box 1 and into the storage box to store the detection reagent.

[0042] The detection method of the primer-probe detection kit for digestive tract tumor markers comprises the following steps: Step 1: Place multiple detection reagents in the storage tank 101, and under the action of the pushing component, control one of the detection reagents to be located between the two card plates 16 through the pushing plate 5; Step 2: At this time, the pressing and rotating mechanism can be controlled by human power to move, thereby driving the rotating and pushing mechanism to move. Under the action of the rotating and pushing mechanism, the two clamping plates 16 are controlled by the clamping and regulating component to move toward each other to fix the detection reagent and rotate around the central axis of the fixed disk 7; Step 3: When the card plate 16 moves to the desired position, the two card plates 16 move away from each other under the action of the clamping and regulating assembly, and the trigger follower mechanism moves under the action of the pressing and rotating mechanism to control the second inclined plate 29 and the second limiting wheel 30 to move away from each other. The second inclined plate 29 will control the detection reagent to move out of the detection box 1 to facilitate the user to take it; Step 4: When the detection is completed, continue to press the rotating mechanism to move, and place the detection reagent between the two card plates 16 again. When the detection reagent moves to the position that cooperates with the second limiting wheel 30, under the action of the second limiting wheel 30, the detection reagent is pushed out of the detection box 1, and the above steps are repeated to achieve the purpose of automatically storing and accessing the detection reagent.

[0043] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0044] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. Primer probe detection kit for digestive tract tumor markers, include: A detection box (1), and a storage slot (101) provided in the detection box (1), a hollow rod (6) being installed in the detection box (1), a first through slot (601) being provided on the circumferential outer wall of the hollow rod (6), and a fixing plate (7) being provided at the end of the hollow rod (6); It is characterized by further comprising: A pushing component is arranged in the storage groove (101), and a pushing plate (5) is connected to the pushing component; A rotary push mechanism is arranged on the fixed disk (7), the rotary push mechanism is provided with a clamping and regulating component, the clamping and regulating component is connected to symmetrically arranged clamping plates (16), and the clamping and regulating component can adjust the distance between the two clamping plates (16) when the rotary push mechanism moves; The pressing and rotating mechanism is arranged in the detection box (1) and connected to the rotating pushing mechanism. The detection box (1) is also provided with a trigger follower mechanism connected to the pressing and rotating mechanism. The trigger follower mechanism is connected to a second inclined plate (29) and a second limiting wheel (30). The pressing and rotating mechanism can drive the rotating pushing mechanism to move. The pressing and rotating mechanism can also drive the second inclined plate (29) and the second limiting wheel (30) to move towards or away from each other through the trigger follower mechanism.

2. The primer-probe detection kit for digestive tract tumor markers according to claim 1, It is characterized in that The pushing assembly comprises a fixed sleeve (2) installed in the storage groove (101) and arranged symmetrically, a movable rod (3) is slidably installed in the fixed sleeve (2), the movable rod (3) is connected to the push plate (5), and a first spring (4) abutting against the push plate (5) is sleeved on the fixed sleeve (2) and the movable rod (3).

3. The primer-probe detection kit for digestive tract tumor markers according to claim 1, It is characterized in that The rotary pushing mechanism comprises a rotary sleeve (8) rotatably mounted on the fixed disk (7), a rotary disk (9) being arranged on the rotary sleeve (8), the clamping plate (16) being rotatably connected to the rotary disk (9), a plurality of slide grooves (901) being equidistantly distributed on a circumference being provided on the rotary disk (9), a receiving ring (10) being arranged in the slide groove (901), and a guide assembly connected to the slide groove (901) being arranged on the fixed disk (7).

4. The primer-probe detection kit for digestive tract tumor markers according to claim 3, It is characterized in that The guide assembly comprises an annular groove (701) and an inclined groove (702) formed on the fixed plate (7); a sliding block (11) is slidably mounted in the sliding groove (901); a limiting column (24) is provided on a side wall of the sliding block (11); and the limiting column (24) is slidably connected to the annular groove (701) and the inclined groove (702).

5. The primer-probe detection kit for digestive tract tumor markers according to claim 4, It is characterized in that The clamping and regulating assembly comprises a support sleeve (12) mounted on the sliding block (11); a clamping groove (1201) is formed on an outer wall of the support sleeve (12); a support rod (13) is slidably mounted in the support sleeve (12); a first limit block (1301) slidably connected to the clamping groove (1201) is provided on the support rod (13); a second spring (14) is sleeved on the support sleeve (12) and the support rod (13); a symmetrically arranged connecting rod (15) is hinged at the end of the support rod (13); and the connecting rod (15) is hinged to the clamping plate (16).

6. The primer-probe detection kit for digestive tract tumor markers according to claim 3, It is characterized in that The pressing and rotating mechanism comprises a guide rod (18) slidably mounted on the detection box (1), a pressure plate (17) being provided at the end of the guide rod (18), a push ring (19) being provided on the guide rod (18), the push ring (19) being slidably connected to the rotating sleeve (8), and a driven component connected to the push ring (19) being provided on the rotating sleeve (8).

7. The primer-probe detection kit for digestive tract tumor markers according to claim 6, It is characterized in that The driven assembly comprises a guide groove formed on the outer wall of the rotating sleeve (8); a second limit block (1901) slidably connected to the guide groove is provided on the inner wall of the pushing ring (19); and a third spring (20) abutting against the pushing ring (19) is sleeved on the rotating sleeve (8).

8. The primer-probe detection kit for digestive tract tumor markers according to claim 7, It is characterized in that The trigger follower mechanism comprises a second through slot (805) provided on the outer wall of the rotating sleeve (8), a limiting ring (21) abutting against the pushing ring (19) being slidably mounted in the second through slot (805), a limiting rod (22) being rotatably mounted on the limiting ring (21), a first inclined plate (23) slidably connected to the first through slot (601) being provided at the end of the limiting rod (22), and an elastic component connected to the first inclined plate (23) being provided in the detection box (1).

9. The primer-probe detection kit for digestive tract tumor markers according to claim 8, It is characterized in that The elastic component comprises a guide sleeve (25) which is installed in the detection box (1) and is symmetrically arranged. A receiving rod (26) is slidably installed in the guide sleeve (25). A first limiting wheel (28) is arranged at one end of the receiving rod (26). The other end of the receiving rod (26) is respectively connected to the second inclined plate (29) and the second limiting wheel (30). The first limiting wheel (28) abuts against the first inclined plate (23). A fourth spring (27) abuts against the guide sleeve (25) is sleeved on the receiving rod (26).

10. A detection method of a primer-probe detection kit for digestive tract tumor markers, using the primer-probe detection kit for digestive tract tumor markers according to any one of claims 1 to 9, It is characterized in that The following steps are involved: Step 1: placing a plurality of detection reagents in the storage tank (101), and under the action of the pushing component, controlling one of the detection reagents to be located between the two card plates (16) through the pushing plate (5); Step 2: At this time, the pressing and rotating mechanism can be controlled by human power to move, thereby driving the rotating and pushing mechanism to move. Under the action of the rotating and pushing mechanism, the two clamping plates (16) are controlled by the clamping and regulating component to move in a direction close to each other, so as to fix the detection reagent and rotate around the central axis of the fixed disk (7); Step 3: When the card plate (16) moves to the desired position, the two card plates (16) move in directions away from each other under the action of the clamping and regulating component, and the trigger follower mechanism moves under the action of the pressing and rotating mechanism to control the second inclined plate (29) and the second limiting wheel (30) to move in directions away from each other. The second inclined plate (29) will control the detection reagent to move toward the outside of the detection box (1) to facilitate the user to take it; Step 4: After the test is completed, continue to press the rotating mechanism to move, and place the test reagent between the two clamping plates (16) again. When the test reagent moves to a position that cooperates with the second limiting wheel (30), the test reagent is pushed out of the test box (1) under the action of the second limiting wheel (30), and the above steps are repeated, thereby achieving the purpose of automatically storing and retrieving the test reagent.

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