Container for microorganism concentration culture detection, detection device and method

Abstract

The invention provides a container for culturing and detecting microorganism concentration, a detection device and a method, relates to the field of microorganism detection, and aims to solve the problem that pollution is easily caused as a culture bottle still needs to be unsealed for sampling when a cuvette is used for measuring the concentration of a suspension with a relatively high OD value at present. Microorganisms can be cultured, the concentration can be directly detected, light beams respectively pass through the suspension in the transmission parts, different transmission parts correspond to different optical paths, a plurality of detection positions are formed, the detection requirement when the OD value is high can be met, meanwhile, due to the fact that the transmission parts are used for directly detecting the suspension in the container in the unsealed state, the detection accuracy is high, and the detection accuracy is high. And the pollution problem caused by unsealing is avoided.

Description

Technical Field

[0001] The present invention relates to the field of microorganism detection, and in particular to a container, a detection device and a method for microorganism concentration culture detection. Background Art

[0002] The concentration of suspensions is generally determined by spectrophotometry. The principle of this detection method is that when a light beam passes through a suspension, its transmittance is reduced due to scattering or absorption. When the concentration of the suspension is low, the transmittance of light obeys the Beer-Lambert law. The concentration of particles is proportional to the optical density (OD value) and inversely proportional to the transmittance within a certain range. The spectrophotometric method is simple and fast, but it can only accurately and directly measure the concentration of particle suspensions with an OD value not exceeding 1.0. When the concentration of particles is high, due to the secondary reflection of light, the Beer-Lambert law will no longer apply, the OD value reading will be significantly lower than the actual value, and the higher the concentration, the greater the deviation.

[0003] Various microbial culture fluids also belong to particulate suspensions. When monitoring the concentration of cultivated microorganisms, the microbiology laboratory needs to open the sealed culture bottle, draw out a certain amount of microbial culture fluid, and pour it into a cuvette, put the cuvette into the sample chamber of the spectrophotometer, measure according to the instrument operation guide, and determine the microbial concentration value based on the standard curve combined with the measurement results. For suspensions with higher OD values, it is necessary to dilute until the OD value drops within the above range to measure more accurately. The operation is cumbersome, and the readings of dilution and non-diluted will jump, with poor stability, affecting accuracy. A measuring cuvette and a measuring method for the concentration of a particulate suspension are disclosed in a Chinese patent (publication number CN117129423A). When the actual OD value of the particulate suspension is far more than 1.0, the concentration of the liquid can still be directly and accurately detected without dilution, thereby greatly improving the efficiency and accuracy of the detection. Although the problem of deviation introduced by the diluted suspension has been solved, after sampling and testing, if the concentration does not meet the standard, the culture bottle needs to be re-sealed to continue the culture. When the sealed culture bottle is opened, phage contamination is likely to be introduced, resulting in culture failure. It is difficult to accurately detect the concentration of a suspension with a high OD value without opening the bottle. Summary of the invention

[0004] The purpose of the present invention is to provide a container, a detection device and a method for culturing and detecting the concentration of microorganisms in view of the defects of the prior art. The container is provided with a protrusion on the bottom and a plurality of transmission parts, which can culture microorganisms and directly detect the concentration. The light beams pass through the suspension in the transmission parts respectively. Different transmission parts correspond to different optical paths, forming a plurality of detection positions, which can meet the detection requirements when the OD value is high. At the same time, since the transmission part is used to directly detect the suspension inside the container in the unopened state, the pollution problem introduced by opening is avoided.

[0005] The first object of the present invention is to provide a container for microbial concentration culture detection, which adopts the following scheme:

[0006] The invention comprises a container having a cavity therein, the container being provided with a convex portion protruding from the bottom surface thereof, the convex portion being made of a light-transmitting material, a plurality of transmission portions being formed on the convex portion, each transmission portion being provided with a detection surface for transmitting a light beam, the cavity extending into each transmission portion, respectively forming a detection area, so that the light beam passes through the liquid in the detection area.

[0007] Furthermore, in the transmission direction of the light beam, the detection areas in at least two transmission parts have different lengths, so that the corresponding transmission parts form different optical paths.

[0008] Furthermore, the optical paths formed by all the transmission parts on the convex part are different, and the detection surface is perpendicular to the transmission direction of the light beam.

[0009] Furthermore, the convex portion is annular, the inner circle of the convex portion forms a concave portion, the detection surface is distributed on the inner circle and the outer circle of the annular convex portion, and all the transmission portions are distributed at intervals on the annular convex portion.

[0010] Furthermore, the convex portion is coaxially distributed with the bottom surface of the container, and the detection surface is perpendicular to the tangential distribution of the convex portion where the detection surface is located.

[0011] The second object of the present invention is to provide a detection device using the container for microorganism concentration culture detection as described in the first object.

[0012] The device further includes a light source unit and a detection unit. The light source unit can emit a light beam to the transmission unit, and the detection unit measures the light beam from the detection unit.

[0013] Furthermore, the light source unit includes a plurality of light sources, which are distributed in a one-to-one correspondence with the transmission unit; the detection unit includes a plurality of detection elements, which are distributed in a one-to-one correspondence with the transmission unit; and the transmission unit is located between the corresponding light sources and detection elements.

[0014] The third object of the present invention is to provide a method for detecting a container for culture and detecting microorganism concentration as described in the first object, comprising:

[0015] The liquid to be tested is placed in the cavity of the container, and the liquid fills the testing area;

[0016] Irradiating a detection light beam to the detection surface on one side of at least two transmission parts, so that the light beam passes through the transmission part and the liquid in the detection area thereof;

[0017] Measure the light beam passing through the transmission part and the detection area, and calculate the concentration of the liquid in different transmission parts;

[0018] Based on the liquid concentration obtained at the detection position, the concentration of the measured liquid in the container is obtained.

[0019] Furthermore, when calculating the concentration of the liquid to be measured in the container, the concentration of the liquid to be measured in the container is calculated by integrating the liquid concentrations obtained at multiple detection positions.

[0020] Compared with the prior art, the present invention has the following advantages and positive effects:

[0021] (1) In view of the problem that when a cuvette is currently used to measure the concentration of a suspension with a high OD value, the culture bottle still needs to be opened for sampling, which is easy to cause contamination. A container with a protrusion on the bottom and multiple transmission parts is used, which can culture microorganisms and directly detect the concentration. The light beam passes through the suspension in the transmission part respectively. Different transmission parts correspond to different optical paths, forming multiple detection positions, which can meet the detection needs when the OD value is high. At the same time, since the transmission part is used to directly detect the suspension inside the container in the unopened state, the contamination problem caused by opening is avoided.

[0022] (2) By setting up multiple transmission parts to form multiple detection positions, after detection, it is possible to assign weights based on the detection results of each position, and use the weighted average as the concentration detection result. This not only solves the problem of low OD readings when the liquid concentration is high, but also solves the problem of jumps between diluted data and non-diluted data. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0024] Figure 1 Schematic diagram of the container for culture and detection of microorganism concentration in Examples 1-3 of the present invention.

[0025] Figure 2 This is a schematic diagram of the bottom structure of the container for microbial concentration culture detection in Examples 1-3 of the present invention.

[0026] Figure 3 It is a cross-sectional schematic diagram of the microorganism concentration culture detection container in Examples 1-3 of the present invention.

[0027] Among them, 1. container, 2. concave part, 3. convex part, 4. fourth transmission part, 5. fourth optical path, 6. second transmission part, 7. second optical path, 8. first transmission part, 9. first optical path, 10. third transmission part, 11. third optical path, 12. cavity. DETAILED DESCRIPTION

[0028] Example 1

[0029] In a typical embodiment of the present invention, Figure 1-Figure 3 As shown, a container for culture and detection of microbial concentration is provided.

[0030] Traditional microbial concentration detection methods usually require opening the container 1 for sampling. This process is prone to introduce external contamination, affecting the accuracy of the test results. In addition, during the microbial cultivation process, as the microbial concentration increases, the OD (optical density) value of the suspension will gradually increase. Traditional detection methods often face the problem of reduced accuracy when the OD value is high. Based on this, the present embodiment provides a microbial concentration culture detection container that can cultivate microorganisms and directly detect the concentration. By forming detection positions with different optical paths through multiple transmission parts, it can effectively respond to the detection needs of high OD values ​​and improve the accuracy and reliability of the detection. The transmission part can form a detection position for the light beam to pass through, allowing the internal suspension to be directly detected without opening the container 1, effectively avoiding the pollution problem introduced by opening.

[0031] like Figure 1 As shown, the main body of the container for microbial concentration culture detection is a container 1, which is used to contain liquid, such as for culturing microorganisms and performing concentration detection on microbial suspensions, and has a cavity 12. The container 1 has an opening for easy injection and pouring of the internal liquid. The opening of the container 1 can be sealed as needed to form a closed cavity 12 in the container 1.

[0032] The container 1 is provided with a convex portion 3 protruding from its bottom surface, such as Figure 3 As shown, it is made of a light-transmitting material so that the light beam can penetrate. In order to meet the needs of multiple position detection, multiple transmission parts are formed on the convex part 3 as detection positions, and each transmission part has a detection surface for the light beam to transmit. The detection light beam acts on the liquid in the cavity 12 after passing through the detection surface and the wall of the container 1 corresponding to the transmission part. The microorganisms in the liquid absorb the light beam of some wavelengths and transmit the light beam out of the transmission part and are collected by the receiver, thereby realizing detection.

[0033] The multiple transmission parts arranged on the convex part 3 enable the light beam to pass through different areas in the cavity 12. Each transmission part and the part of the cavity 12 below it together form a detection area, which is a part of the cavity 12, and the liquid in the cavity 12 is also filled into the detection area. According to the detection requirements, different optical paths can be configured for the transmission parts according to the different distribution positions of the transmission parts.

[0034] like Figure 2 As shown, in the transmission direction of the light beam, the detection areas in at least two transmission parts have different lengths, so that the corresponding transmission parts form different optical path lengths. Under the premise that the wall thickness of the container 1 is fixed, due to the different lengths of the detection areas, the lengths of the transmission parts corresponding to the detection areas are also different, and the optical path lengths experienced by the light beam when passing through different transmission parts are also different.

[0035] The light beam is emitted from an external light source, enters the container 1 through the detection surface of the transmission part, passes through the liquid in the detection area, and is then received by the receiver. Since different transmission parts correspond to different optical paths, the intensity of the received light signal will also be different. Similarly, when the concentration of the liquid in the cavity 12 is different, the intensity of the light signal received after the light beam passes through the same transmission part will also be different.

[0036] The design of container 1 allows the internal suspension to be directly tested without opening the seal, avoiding external contamination introduced by unsealing sampling in traditional methods, thereby ensuring the accuracy and reliability of the test results. At the same time, there is no need to perform cumbersome sampling and pretreatment steps, and the test can be directly performed through the transmission part on the container 1, which greatly simplifies the operation process and improves the detection efficiency. By setting multiple transmission parts and forming detection positions with different optical paths, the concentration changes of microorganisms in the suspension can be more accurately reflected, especially when the OD value is high, a high detection accuracy can be maintained.

[0037] It should be noted that the appearance of the container 1 can be adjusted according to the needs, such as a triangular flask, a conical flask, etc., and other shapes suitable for microbial culture can also be used. The number and distribution position of the transmission parts on the container 1 can also be adjusted according to the actual detection needs. The number of transmission parts can be configured to be 3, 4, etc., and the distribution position can be arranged at equal intervals or unequal intervals, which is suitable for the detection of microorganisms of different types and concentrations.

[0038] In the transmission direction of the light beam, the detection areas in at least two transmission parts have different lengths, and the corresponding transmission parts form different optical paths. Figure 2 As shown, four transmission parts are provided, namely the first transmission part 8, the second transmission part 6, the third transmission part 10 and the fourth transmission part 4. The optical paths formed by all the transmission parts on the convex part 3 are different. The detection surface is perpendicular to the transmission direction of the light beam. The four transmission parts correspond to the first optical path 9, the second optical path 7, the third optical path 11 and the fourth optical path 5, so that each transmission part can provide a unique detection viewing angle and optical path, thereby improving the diversity and accuracy of detection.

[0039] The first optical path 9 corresponding to the first transmission part 8 can be 10 mm, the second optical path 7 corresponding to the second transmission part 6 can be 5 mm, the third optical path 11 corresponding to the third transmission part 10 can be 2 mm, and the fourth optical path 5 corresponding to the fourth transmission part 4 can be 1 mm. In other optional embodiments, the optical paths corresponding to the four transmission parts can be selected from other parameters, such as 7 mm, 4 mm, 3 mm, etc.

[0040] The different lengths of the detection areas make the light beam experience different path lengths (i.e., optical path lengths) when passing through these areas. The difference in optical path lengths directly affects the attenuation of the light signal after passing through the suspension, and thus reflects different information about the concentration of microorganisms in the suspension. By having multiple detection positions with different optical path lengths, high-concentration liquids can also meet the transmission requirements through a shorter optical path length, thereby improving the accuracy and reliability of the detection.

[0041] The detection surface is perpendicular to the direction of light beam transmission, which meets the requirements of spectrophotometric detection and helps to ensure that the light beam can pass through the detection area vertically, reducing errors caused by light beam tilt.

[0042] like Figure 2 As shown, the convex portion 3 is annular, and the inner circle of the convex portion 3 forms the concave portion 2. The detection surfaces are distributed on the inner circle and the outer circle of the annular convex portion 3, that is, the detection surface on one side of the transmission portion faces the outside of the container 1, and the detection surface on the other side faces the concave portion 2. Corresponding to the injection and emission of the light beam, all the transmission portions are distributed at intervals on the annular convex portion 3.

[0043] The design of the annular protrusion 3 enables the detection surface to be distributed at different positions of the container 1, thereby realizing detection of different areas of the suspension, improving the comprehensiveness and uniformity of the detection. It is also convenient to arrange multiple detection elements and improve the compactness of the device. The spaced distribution of the transmission part on the annular protrusion 3 helps to avoid overlap and interference of detection positions, ensuring that each detection position can provide independent and accurate detection information.

[0044] The convex part 3 is coaxially distributed with the bottom surface of the container 1, and the detection surface is perpendicular to the tangential distribution of the convex part 3 where it is located. The coaxial distribution of the convex part 3 with the bottom surface of the container 1 helps to maintain the stability and balance of the container 1, while ensuring that the light beam can accurately pass through the detection area. At the same time, the concave part 2 is provided to facilitate the installation of the detection element at the position of the concave part 2.

[0045] The tangential distribution of the detection surface perpendicular to the convex portion 3 where it is located helps to ensure that the light beam can maintain a stable path and direction when passing through the detection area, reducing the error caused by the change of the light beam direction. At the same time, this distribution method also helps to improve the uniformity and consistency of detection.

[0046] Example 2

[0047] In another typical embodiment of the present invention, Figure 1-Figure 3 As shown, a detection device is provided.

[0048] By using the microorganism concentration culture detection container as in Example 1, the detection device realizes non-contact, high-precision detection of the concentration of the microorganism suspension in the container 1 by integrating the light source unit and the detection unit. The light source unit can emit a light beam to the transmission unit, and the detection unit measures the light beam from the detection unit.

[0049] The light source unit includes multiple light sources, which are distributed one by one with the transmissive parts on the container 1. Each light source is responsible for emitting a light beam to the corresponding transmissive part to ensure that the light beam can accurately pass through the detection area. The main function of the light source unit is to provide a stable and uniform light source to ensure that the intensity and direction of the light beam are consistent during the detection process, thereby improving the accuracy and reliability of the detection.

[0050] The detection unit includes a plurality of detection elements, which are also distributed one by one with the transmission parts on the container 1. Each detection element is responsible for receiving and measuring the light beam from the corresponding transmission part. The main function of the detection unit is to receive the light signal after passing through the suspension and accurately measure it. By comparing the intensity of the light signal transmitted from different transmission parts, the concentration distribution of microorganisms in the suspension can be calculated.

[0051] A one-to-one correspondence is formed between the light source and the detection element and the transmission part, and each transmission part has an independent light source and detection element to cooperate with each other, thereby improving the independence and accuracy of the detection.

[0052] The transmission part is located between the corresponding light source and the detection part, forming a complete optical path system. The light beam is emitted from the light source, passes through the transmission part and enters the suspension, and then is received by the detection part after passing through the suspension. In this process, the attenuation degree of the light beam directly reflects the concentration information of the microorganisms in the suspension.

[0053] Through the cooperation of multiple light sources and detection elements, and the different optical path detection positions formed by the transmission part, the detection device can achieve high-precision detection of the concentration of microorganisms in the suspension. The entire detection process does not require opening the container 1, avoiding the contamination problem caused by opening, and also simplifying the operation process.

[0054] Since the number of light sources and detection parts can be adjusted according to actual needs, the detection device has high flexibility and scalability and is suitable for detecting microorganisms of different types and concentrations. By integrating the light source part and the detection part, and combining the container for microbial concentration culture detection, high-precision, non-contact detection of microbial concentration in suspension is achieved. It not only improves the accuracy and reliability of the detection, but also simplifies the operation process and reduces the risk of contamination.

[0055] Example 3

[0056] In another typical embodiment of the present invention, Figure 1-Figure 3 As shown, a method for detecting a container for culturing and detecting microorganism concentration is provided, using the container for culturing and detecting microorganism concentration as in Example 1.

[0057] The liquid to be tested is placed in the cavity 12 of the container 1, and the liquid fills the testing area;

[0058] Irradiating a detection light beam to the detection surface on one side of at least two transmission parts, so that the light beam passes through the transmission part and the liquid in the detection area thereof;

[0059] Measure the light beam passing through the transmission part and the detection area, and calculate the concentration of the liquid in different transmission parts;

[0060] Based on the liquid concentration acquired at the detection position, the concentration of the measured liquid in the container 1 is obtained.

[0061] When calculating the concentration of the liquid to be measured in the container 1, the concentration of the liquid to be measured in the container is calculated by integrating the concentrations of the liquid obtained at multiple detection positions.

[0062] In this embodiment, the E. coli solution cultured in container 1 is used as an example for explanation. The detection light beam adopts 600 nanometers to detect the OD values ​​of the 10 mm, 5 mm, 2 mm and 1 mm optical paths corresponding to the four transmission parts. A weighted method is used to assign different weights to different optical paths at different concentrations, which solves the problem of low OD readings at high concentrations and the problem of jumps between diluted data and non-diluted data.

[0063] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A container for culture and detection of microorganism concentration, characterized in that: The invention comprises a container having a cavity therein, the container being provided with a convex portion protruding from the bottom surface thereof, the convex portion being made of a light-transmitting material, a plurality of transmission portions being formed on the convex portion, each transmission portion being provided with a detection surface for transmitting a light beam, the cavity extending into each transmission portion, respectively forming a detection area, so that the light beam passes through the liquid in the detection area.

2. The container for culture and detection of microorganism concentration according to claim 1, characterized in that: In the light beam transmission direction, the detection areas in at least two transmission parts have different lengths, so that the corresponding transmission parts form different optical paths.

3. The container for culture and detection of microorganism concentration according to claim 2, characterized in that: The optical paths formed by all the transmission parts on the convex part are different, and the detection surface is perpendicular to the transmission direction of the light beam.

4. The container for culture and detection of microorganism concentration according to claim 1, characterized in that: The convex part is annular, the inner circle of the convex part forms a concave part, the detection surface is distributed on the inner circle and the outer circle of the annular convex part, and all the transmission parts are distributed at intervals on the annular convex part.

5. The container for culture and detection of microorganism concentration according to claim 4, characterized in that: The convex part is coaxially distributed with the bottom surface of the container, and the detection surface is perpendicular to the tangential distribution of the convex part where the detection surface is located.

6. A detection device, characterized in that: A container for culturing and detecting microorganism concentration as claimed in any one of claims 1 to 5 is used.

7. The detection device according to claim 6, characterized in that The device further includes a light source unit and a detection unit. The light source unit can emit a light beam to the transmission unit, and the detection unit measures the light beam from the detection unit.

8. The monitoring device according to claim 7, characterized in that: The light source unit includes a plurality of light sources, which are distributed in one-to-one correspondence with the transmission unit; the detection unit includes a plurality of detection elements, which are distributed in one-to-one correspondence with the transmission unit, and the transmission unit is located between the corresponding light source and detection element.

9. A method for detecting a container for culturing and detecting microorganism concentration, using the container for culturing and detecting microorganism concentration as claimed in any one of claims 1 to 5, characterized in that: include: The liquid to be tested is placed in the cavity of the container, and the liquid fills the testing area; Irradiating a detection light beam to the detection surface on one side of at least two transmission parts, so that the light beam passes through the transmission part and the liquid in the detection area thereof; Measure the light beam passing through the transmission part and the detection area, and calculate the concentration of the liquid in different transmission parts; Based on the liquid concentration obtained at the detection position, the concentration of the measured liquid in the container is obtained.

10. The method for detecting a container for culture and detection of microorganism concentration according to claim 9, characterized in that: When calculating the concentration of the measured liquid in the container, the concentration of the measured liquid in the container is calculated by integrating the liquid concentrations obtained at multiple detection positions.

Images