Micro-tumor cell culture device

Abstract

The invention belongs to the technical field of biology, and particularly relates to a micro tumor cell culture device which comprises a culture box, a storage plate, glassware, a connecting structure, an infusion structure and a movable structure, the culture box comprises a box body and a first limiting groove, the movable structure is arranged in the first limiting groove, and the movable structure is arranged on the connecting structure and the infusion structure; an air cylinder stretches out and draws back to drive a glass cover to move upwards, the glass cover leaves a glass carrying plate, original culture liquid leaks to the bottom of a culture dish, the culture liquid in a liquid storage tank cleans the glass carrying plate, then the glass cover moves downwards to the position where the glass cover is embedded with the glass carrying plate, and the glass cover and the glass carrying plate are combined to form a new inner space. A new culture solution is injected, the new culture solution is stored in a space formed by the glass support plate and the glass cover by utilizing a water seal principle, the culture solution is conveniently and safely replaced under the condition of ensuring that the incubator is closed, and bacteria in external air are prevented from infecting tumor cells when the culture solution is replaced.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, in particular to a micro-tumor cell culture device. Background Art

[0002] Tumors have become one of the most difficult diseases to cure for humans today. Cultivating tumor cell growth has the special function of early detection of cell canceration. Tumor cell growth is the key enzyme that controls cell canceration and proliferation, and is an important factor in tumor growth. To prevent and treat tumors early, it is necessary to predict the tumor growth process through cell culture in the blood. When processing cells, they need to be sent to the laboratory for culture, test the cell growth process and lesions, and obtain new tumor cell populations through cell culture for repeated in-depth research, and develop drug reagents that can target tumor cells.

[0003] In the prior art, announcement number CN 115838621 A provides a debridement device, including a culture box body and a culture box cover hinged on one side of the top of the culture box body; the bottom of the culture box body is fixedly connected with an annular tooth plate, the annular tooth plate is provided with a first rotating component and a second rotating component, the second rotating component is slidably connected to the annular tooth plate through the first rotating component, a limiting component is provided on the second rotating component, and the second rotating component is detachably connected with a number of cell culture dishes through the limiting component; the bottom of the culture box body is fixedly connected with a driving motor, the driving motor is transmission-connected with the first rotating component, a heating component is provided in the culture box body, and the cell culture dishes and the heating component are correspondingly arranged. This invention ensures uniform heating during the tumor cell culture process, while preventing the culture fluid from precipitating or solidifying due to long-term static placement, reducing the impact on tumor cell culture, and ensuring the culture efficiency of tumor cells.

[0004] In the above patent, a driving motor is fixedly connected to the bottom of the culture box, and the driving motor is in transmission connection with the first rotating assembly, so that the culture dish moves in a circular motion in the culture box, thereby preventing the culture fluid from precipitating or coagulating due to long-term static placement, thereby reducing the impact on tumor cell culture. However, the culture device still has the following problems:

[0005] When replacing the culture medium of this culture device, the incubator needs to be opened and the hand is then inserted into the incubator to replace the culture medium. During this process, the incubator is connected to the external space, causing the carefully cared-for tumor cells to be infected by bacteria in the outside air.

[0006] Therefore, a micro tumor cell culture device is proposed to solve the above problems. Summary of the invention

[0007] 1. Technical issues to be resolved

[0008] In view of the deficiencies in the prior art, the present invention provides a micro-tumor cell culture device, which solves the problem that when replacing the culture medium in the existing tumor cell culture device, the incubator needs to be opened and the hand needs to be inserted into the incubator to replace the culture medium. During this process, the incubator is connected to the external space, causing the carefully cared-for tumor cells to be infected by bacteria in the outside air.

[0009] (II) Technical solution

[0010] To achieve the above-mentioned object, the present invention provides the following technical solutions: a micro-tumor cell culture device, comprising a culture box, a storage plate, a glass vessel, a connection structure, an infusion structure and a movable structure, the culture box comprising a box body, a first limiting groove, the movable structure is arranged in the first limiting groove, the movable structure is arranged on the connection structure and the infusion structure, the storage plate is arranged inside the box body, the storage plate is provided with a glass vessel, and the glass vessel is located directly below the infusion structure;

[0011] The glassware comprises a culture dish and a glass carrier, and the inner bottom of the culture dish is fixedly connected to the glass carrier;

[0012] The connecting structure comprises a glass cover, a connecting rod and a fixing plate, wherein the two connecting rods are arranged in the first limiting groove, the fixing plate is arranged above the two connecting rods, and the glass cover is arranged directly below the two connecting rods;

[0013] The infusion structure comprises a glass tube, a valve and a liquid storage tank. The glass tube is arranged directly above the glass cover, and the valve and the liquid storage tank are arranged above the fixed plate.

[0014] Preferably, the lower ends of the two connecting rods are fixedly connected to the glass cover, and the lower side of the glass cover is engaged with the glass carrier plate, the upper ends of the two connecting rods are fixedly connected to the fixed plate, the upper end of the cylinder is provided below the rear side of the fixed plate, and the upper end of the cylinder is fixedly connected to the fixed plate, and the lower end of the cylinder is fixedly connected to the top of the box body.

[0015] Preferably, the upper side of the glass tube is snap-connected with the fixed plate, and the valve is arranged on the upper side of the glass tube, the upper end of the glass tube is connected to the liquid storage tank, and the lower end of the glass tube is connected to the glass cover, a motor is arranged on the top of the outer side of the glass cover, and the motor is fixedly connected to the top of the glass cover, an agitator is arranged on the top of the inner side of the glass cover, and the output end of the motor is rotatably connected to the agitator.

[0016] Preferably, the storage plate includes a movable plate, a sliding rod, a groove, a first sliding groove, a second sliding groove, a square groove and a second limiting groove, both sides of the movable plate are fixedly connected to the sliding rod, and the inner walls on both sides of the box body are respectively fixed with track rods, and the track rods are slidably connected to the sliding rod.

[0017] Preferably, the groove and the first slide groove are opened on the movable plate, and the two first slide grooves are symmetrically arranged on the sides of the groove. A clamping block and a second spring are provided in the first slide groove, and the clamping block slides in the first slide groove. One end of the clamping block is engaged with one end of the second spring, and the other end of the second spring is engaged with the groove wall of the first slide groove.

[0018] Preferably, the second slide groove and the second limit groove are opened in the movable plate, and the two second limit grooves are arranged on both sides of the second slide groove. A pushing structure is provided in the second slide groove, and the pushing structure includes a cross plate and a card block. The cross plate slides up and down in the second slide groove, and the two card blocks are respectively fixedly connected to the two sides of the cross plate. The card block is slidably connected to the second limit groove, and the card block slides up and down in the second limit groove.

[0019] Preferably, a linkage structure is also provided in the second slide groove, and the linkage structure includes a rotating block, a rotating shaft and a fixed shaft. The rotating block rotates in the second slide groove, the rear end of the rotating block is fit-connected to the cross plate, the front end of the rotating block is fixedly connected to both ends of the fixed shaft, a square rod is connected to the fixed shaft, and the lower end of the square rod is rotatably connected to the fixed shaft, the two sides of the rotating block are fixedly connected to the rotating shaft, and the two rotating shafts are rotatably connected to the groove wall of the second slide groove.

[0020] Preferably, the square groove is provided on the movable plate, the square rod is slidably connected to the square groove, the square rod is arranged in a square column-shaped structure, and the square rod moves up and down in the square groove.

[0021] Preferably, a first spring is sleeved on the upper portion of the square rod, and the first spring is disposed above the movable plate, the upper end of the first spring is fitted and connected to the upper end of the square rod, and the lower end of the first spring is fitted and connected to the upper surface of the movable plate.

[0022] Preferably, the movable structure includes a movable block and a rubber ring, the movable block is engaged with the connecting rod and the glass tube, the movable block is arranged in the first limiting groove, and the movable block moves up and down in the first limiting groove, the rubber ring is fitted on the movable block, and the rubber ring is fitted and connected to the groove wall of the first limiting groove.

[0023] (III) Beneficial effects

[0024] Compared with the prior art, the present invention provides a micro-tumor cell culture device having the following

[0025] Beneficial effects:

[0026] 1. A micro-tumor cell culture device, through the expansion and contraction of the cylinder, drives the fixed plate to move upward, drives the connecting rod and the glass cover to move upward, so that the glass cover leaves the glass carrier, and the original culture solution leaks to the bottom of the culture dish, opens the valve to drain the culture solution in the liquid storage tank, and uses the new culture solution to clean the glass carrier, and then moves the glass cover downward to a position where it is embedded with the glass carrier, so that the glass cover and the glass carrier are combined to form a new internal space, injects new culture solution, and uses the water seal principle to store the new culture solution in the space formed by the glass carrier and the glass cover. Under the condition of ensuring that the incubator is airtight, the culture solution can be replaced conveniently and safely, and bacteria in the outside air can be prevented from infecting tumor cells when the culture solution is replaced.

[0027] 2. The micro-tumor cell culture device pushes the two clamping blocks to move toward the middle by means of the second spring, thereby clamping and fixing the culture dish in the groove so that the culture dish is directly below the glass cover.

[0028] 3. This micro-tumor cell culture device moves the movable plate outward by pulling the movable plate to move outward, and presses the square rod to move the square rod downward in the square groove. The downward movement of the square rod drives the rotating block to rotate around the rotating shaft. The rotating block drives the horizontal plate to move upward in the second sliding groove, and pushes the culture dish upward to leave the groove, so that the culture dish is easy to take out.

[0029] 4. This micro tumor cell culture device uses a motor to drive the stirrer to rotate inside the glass cover to stir the culture solution between the glass cover and the glass carrier plate, thereby preventing the culture solution from precipitating or coagulating due to long-term static placement, thereby reducing the impact on tumor cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0031] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0032] Figure 2 It is a schematic diagram of the three-dimensional structure of the incubator of the present invention;

[0033] Figure 3 It is a schematic diagram of the three-dimensional structure of the storage board of the present invention;

[0034] Figure 4 is a schematic cross-sectional view of the three-dimensional structure of the storage board of the present invention;

[0035] Figure 5 It is a three-dimensional structural schematic diagram of the linkage structure and the pushing structure of the present invention;

[0036] Figure 6 It is a schematic diagram of the three-dimensional structure of the clamping block of the present invention;

[0037] Figure 7 It is a schematic diagram of the three-dimensional structure of the connection structure, the infusion structure and the glass container of the present invention.

[0038] In the figure:

[0039] 1. Cultivation box; 101. Box body; 102. First limiting groove;

[0040] 2. Track rod;

[0041] 3. Storage plate; 301. Movable plate; 302. Sliding rod; 303. Groove; 304. First sliding groove; 305. Second sliding groove; 306. Square groove; 307. Second limiting groove;

[0042] 4. Square rod; 5. First spring;

[0043] 6. Glassware; 601. Petri dish; 602. Glass carrier plate;

[0044] 7. Connecting structure; 701. Glass cover; 702. Connecting rod; 703. Fixing plate;

[0045] 8. Infusion structure; 801. Glass tube; 802. Valve; 803. Liquid storage tank;

[0046] 9. Cylinder;

[0047] 10. movable structure; 1001. movable block; 1002. rubber ring;

[0048] 11. Clamp block; 12. Second spring;

[0049] 13. linkage structure; 1301. rotating block; 1302. rotating shaft; 1303. fixed shaft;

[0050] 14. Pushing structure; 1401. Horizontal plate; 1402. Card block

[0051] 15. Agitator; 16. Motor. DETAILED DESCRIPTION

[0052] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0053] Specific examples are given below.

[0054] See also Figure 1-Figure 7The present invention provides a micro-tumor cell culture device, comprising a culture box 1, a storage plate 3, a glass vessel 6, a connection structure 7, an infusion structure 8 and a movable structure 10, wherein the culture box 1 comprises a box body 101 and a first limiting groove 102, wherein the movable structure 10 is arranged in the first limiting groove 102, wherein the movable structure 10 is arranged on the connection structure 7 and the infusion structure 8, wherein the storage plate 3 is arranged inside the box body 101, wherein the glass vessel 6 is arranged on the storage plate 3, and wherein the glass vessel 6 is located directly below the infusion structure 8, wherein the glass vessel 6 comprises a culture dish 601 and a glass carrier plate 602, wherein the inner bottom of the culture dish 601 is fixedly connected to the glass carrier plate 602, wherein the connection structure 7 comprises a glass cover 701, a connecting rod 702 and a fixing plate 70 3. The two connecting rods 702 are arranged in the first limiting groove 102, the fixing plate 703 is arranged above the two connecting rods 702, the glass cover 701 is arranged directly below the two connecting rods 702, the infusion structure 8 includes a glass tube 801, a valve 802 and a liquid storage tank 803, the glass tube 801 is arranged directly above the glass cover 701, the valve 802 and the liquid storage tank 803 are arranged above the fixing plate 703, the lower ends of the two connecting rods 702 are fixedly connected to the glass cover 701, and the lower side of the glass cover 701 is engaged with the glass carrier 602, the upper ends of the two connecting rods 702 are fixedly connected to the fixing plate 703, the upper end of the cylinder 9 is arranged below the rear side of the fixing plate 703, and the cylinder 9 The upper end of the cylinder 9 is fixedly connected to the fixed plate 703, the lower end of the cylinder 9 is fixedly connected to the top of the box body 101, the upper side of the glass tube 801 is snap-connected with the fixed plate 703, and the valve 802 is arranged on the upper side of the glass tube 801, the upper end of the glass tube 801 is connected to the liquid storage tank 803, and the lower end of the glass tube 801 is connected to the glass cover 701, the outer top of the glass cover 701 is provided with a motor 16, and the motor 16 is fixedly connected to the top of the glass cover 701, the inner top of the glass cover 701 is provided with a stirrer 15, the output end of the motor 16 is rotatably connected to the stirrer 15, the storage plate 3 includes a movable plate 301, a slide bar 302, a groove 303, a first slide groove 304, a second slide groove 305, a square The first and second limiting grooves 306 and 307 are provided on both sides of the movable plate 301 and are fixedly connected to the slide bar 302. The inner walls of the two sides of the box body 101 are respectively fixed with track rods 2, and the track rods 2 are slidably connected to the slide bar 302. The groove 303 and the first slide groove 304 are provided on the movable plate 301. The two first slide grooves 304 are symmetrically arranged on the sides of the groove 303. A clamping block 11 and a second spring 12 are provided in the first slide groove 304. The clamping block 11 slides in the first slide groove 304. One end of the clamping block 11 is engaged with one end of the second spring 12, and the other end of the second spring 12 is engaged with the groove wall of the first slide groove 304. The second slide groove 305 and the second limiting groove 307 are provided in the movable plate 301.The two second limiting grooves 307 are arranged on both sides of the second slide groove 305. The second slide groove 305 is provided with a pushing structure 14. The pushing structure 14 includes a transverse plate 1401 and a block 1402. The transverse plate 1401 slides up and down in the second slide groove 305. The two blocks 1402 are respectively fixedly connected to both sides of the transverse plate 1401. The blocks 1402 are slidably connected to the second limiting grooves 307. The blocks 1402 slide up and down in the second limiting grooves 307. The second slide A linkage structure 13 is also provided in the groove 305, and the linkage structure 13 includes a rotating block 1301, a rotating shaft 1302 and a fixed shaft 1303. The rotating block 1301 rotates in the second sliding groove 305, and the rear end of the rotating block 1301 is closely connected to the cross plate 1401, and the front end of the rotating block 1301 is fixedly connected to both ends of the fixed shaft 1303. The fixed shaft 1303 is connected to a square rod 4, and the lower end of the square rod 4 is rotatably connected to the fixed shaft 1303. Both sides of the rotating block 1301 The rotating shaft 1302 is fixedly connected, and the two rotating shafts 1302 are rotatably connected with the groove wall of the second sliding groove 305. The square groove 306 is opened on the movable plate 301. The square rod 4 is slidably connected with the square groove 306. The square rod 4 is arranged in a square column shape structure, and the square rod 4 moves up and down in the square groove 306. The upper sleeve of the square rod 4 is provided with a first spring 5, and the first spring 5 is arranged above the movable plate 301. The upper end of the first spring 5 is fitted and connected with the upper end of the square rod 4, and the first The lower end of the spring 5 is connected to the upper surface of the movable plate 301 in a close fit. The movable structure 10 includes a movable block 1001 and a rubber ring 1002. The movable block 1001 is connected to the connecting rod 702 and the glass tube 801 in a snap fit. The movable block 1001 is disposed in the first limiting groove 102, and the movable block 1001 moves up and down in the first limiting groove 102. The rubber ring 1002 is fitted on the movable block 1001, and the rubber ring 1002 is connected to the groove wall of the first limiting groove 102 in a close fit.

[0055] During operation, the cylinder 9 is extended and retracted to drive the fixing plate 703 to move upward, driving the connecting rod 702 and the glass cover 701 to move upward, so that the glass cover 701 leaves the glass carrier 602, causing the original culture solution to leak to the bottom of the culture dish 601, and the valve 802 is opened to drain the culture solution in the liquid storage tank 803, so that the new culture solution cleans the glass carrier 602, and then the glass cover 701 is moved downward to a position where it is embedded with the glass carrier 602, so that the glass cover 701 and the glass carrier 602 are combined into a new internal space, and new culture solution is injected, and the new culture solution is stored in the water seal principle. In the space formed by the glass carrier plate 602 and the glass cover 701, while ensuring that the incubator 1 is airtight, the culture medium can be replaced conveniently and safely. The movable plate 301 is pulled outward to move to the outside of the box body 101, and the square rod 4 is pressed to move the square rod 4 downward in the square groove 306. The downward movement of the square rod 4 drives the rotating block 1301 to rotate around the rotating shaft 1302. The rotating block 1301 moves the horizontal plate 1401 upward in the second slide groove 305, pushing the culture dish 601 upward to leave the groove 303, so that the culture dish 601 is convenient to take out.

[0056] Working principle: When using the micro-tumor cell culture device, firstly, the cancer cell sample is planted on the glass carrier 602 in the culture dish 601, and the movable plate 301 is pulled to move the sliding rod 302 inside and outside the track rod 2, so that the movable plate 301 moves to the outside of the box 101, and the culture dish 601 is embedded in the groove 303. The second spring 12 pushes the two clamping blocks 11 to move toward the middle, clamping and fixing the culture dish 601 in the groove 303, and then the movable plate 301 is pushed into the box 101, so that the culture dish 601 is directly under the glass cover 701 for cultivation. The motor 16 drives the stirrer 15 to rotate in the glass cover 701 to stir the culture solution between the glass cover 701 and the glass carrier 602 to prevent the culture solution from precipitating or solidifying due to long-term static placement. Secondly, when replacing the culture solution, the cylinder 9 is extended and retracted to drive the fixed plate 703 to move upward, driving the connecting rod 702 and the glass cover 701 to move upward, so that the glass The cover 701 leaves the glass carrier 602, so that the original culture solution leaks to the bottom of the culture dish 601, and the valve 802 is opened to drain the culture solution in the liquid storage tank 803, so that the new culture solution cleans the glass carrier 602. After the glass cover 701 is moved downward to a position where it is embedded with the glass carrier 602, the glass cover 701 and the glass carrier 602 are combined to form a new internal space, and new culture solution is injected. The new culture solution is stored in the space formed by the glass carrier 602 and the glass cover 701 by using the water seal principle, and the culture solution is replaced. Next, when taking out the culture sample, the movable plate 301 is pulled out, and the square rod 4 is pressed to move the square rod 4 downward in the square groove 306. The downward movement of the square rod 4 drives the rotating block 1301 to rotate around the rotating shaft 1302. The rotating block 1301 rotates to move the horizontal plate 1401 upward in the second slide groove 305, and the culture dish 601 in the groove 303 is pushed upward, so that the sample in the culture dish 601 is conveniently taken out.

[0057] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A micro-tumor cell culture device, comprising a culture box (1), a storage plate (3), a glass container (6), a connecting structure (7), an infusion structure (8) and a movable structure (10), Features: The incubator (1) comprises a box body (101) and a first limiting groove (102); the movable structure (10) is arranged in the first limiting groove (102); the movable structure (10) is arranged on the connecting structure (7) and the infusion structure (8); the storage plate (3) is arranged inside the box body (101); a glass vessel (6) is arranged on the storage plate (3); and the glass vessel (6) is located directly below the infusion structure (8); The glass vessel (6) comprises a culture dish (601) and a glass carrier plate (602), and the inner bottom of the culture dish (601) is fixedly connected to the glass carrier plate (602); The connection structure (7) comprises a glass cover (701), a connection rod (702) and a fixing plate (703), wherein the two connection rods (702) are arranged in the first limiting groove (102), the fixing plate (703) is arranged above the two connection rods (702), and the glass cover (701) is arranged directly below the two connection rods (702); The liquid infusion structure (8) comprises a glass tube (801), a valve (802) and a liquid storage tank (803); the glass tube (801) is arranged directly above the glass cover (701); and the valve (802) and the liquid storage tank (803) are arranged above the fixed plate (703).

2. A micro-tumor cell culture device according to claim 1, Features: The lower ends of the two connecting rods (702) are fixedly connected to the glass cover (701), and the lower side of the glass cover (701) is engaged with the glass carrier (602). The upper ends of the two connecting rods (702) are fixedly connected to the fixed plate (703). The upper end of the cylinder (9) is arranged below the rear side of the fixed plate (703), and the upper end of the cylinder (9) is fixedly connected to the fixed plate (703). The lower end of the cylinder (9) is fixedly connected to the top of the box body (101).

3. A micro-tumor cell culture device according to claim 1, Features: The upper side of the glass tube (801) is snap-connected with the fixing plate (703), and the valve (802) is arranged on the upper side of the glass tube (801). The upper end of the glass tube (801) is connected with the liquid storage tank (803), and the lower end of the glass tube (801) is connected with the glass cover (701). A motor (16) is arranged on the top of the outer side of the glass cover (701), and the motor (16) is fixedly connected to the top of the glass cover (701). A stirrer (15) is arranged on the top of the inner side of the glass cover (701), and the output end of the motor (16) is rotationally connected to the stirrer (15).

4. A micro-tumor cell culture device according to claim 1, Features: The storage plate (3) comprises a movable plate (301), a sliding rod (302), a groove (303), a first sliding groove (304), a second sliding groove (305), a square groove (306) and a second limiting groove (307); both sides of the movable plate (301) are fixedly connected to the sliding rod (302); the inner walls on both sides of the box body (101) are respectively fixed with track rods (2); and the track rods (2) are slidably connected to the sliding rod (302).

5. A micro-tumor cell culture device according to claim 4, Features: The groove (303) and the first slide groove (304) are opened on the movable plate (301), and the two first slide grooves (304) are symmetrically arranged on the side of the groove (303). A clamping block (11) and a second spring (12) are arranged in the first slide groove (304). The clamping block (11) slides in the first slide groove (304), and one end of the clamping block (11) is snap-connected with one end of the second spring (12), and the other end of the second spring (12) is snap-connected with the groove wall of the first slide groove (304).

6. A micro-tumor cell culture device according to claim 4, Features: The second slide groove (305) and the second limiting groove (307) are opened in the movable plate (301), and the two second limiting grooves (307) are arranged on both sides of the second slide groove (305). A pushing structure (14) is arranged in the second slide groove (305), and the pushing structure (14) comprises a transverse plate (1401) and a clamping block (1402). The transverse plate (1401) slides up and down in the second slide groove (305), and the two clamping blocks (1402) are respectively fixedly connected to the two sides of the transverse plate (1401). The clamping block (1402) is slidably connected to the second limiting groove (307), and the clamping block (1402) slides up and down in the second limiting groove (307).

7. A micro-tumor cell culture device according to claim 4, Features: A linkage structure (13) is also provided in the second slide groove (305), and the linkage structure (13) comprises a rotating block (1301), a rotating shaft (1302) and a fixed shaft (1303). The rotating block (1301) rotates in the second slide groove (305), and the rear end of the rotating block (1301) is closely connected to the cross plate (1401), and the front end of the rotating block (1301) is fixedly connected to both ends of the fixed shaft (1303). A square rod (4) is connected to the fixed shaft (1303), and the lower end of the square rod (4) is rotatably connected to the fixed shaft (1303). The two sides of the rotating block (1301) are fixedly connected to the rotating shaft (1302), and the two rotating shafts (1302) are rotatably connected to the groove wall of the second slide groove (305).

8. A micro-tumor cell culture device according to claim 7, Features: The square groove (306) is provided on the movable plate (301), the square rod (4) is slidably connected to the square groove (306), the square rod (4) is arranged in a square column-shaped structure, and the square rod (4) moves up and down in the square groove (306).

9. A micro-tumor cell culture device according to claim 7, Features: The square rod (4) is sleeved with a first spring (5), and the first spring (5) is arranged above the movable plate (301), the upper end of the first spring (5) is closely connected to the upper end of the square rod (4), and the lower end of the first spring (5) is closely connected to the upper surface of the movable plate (301).

10. The micro-tumor cell culture device according to claim 1, Features: The movable structure (10) comprises a movable block (1001) and a rubber ring (1002); the movable block (1001) is snap-fitted and connected with the connecting rod (702) and the glass tube (801); the movable block (1001) is arranged in a first limiting groove (102), and the movable block (1001) moves up and down in the first limiting groove (102); the rubber ring (1002) is sleeved on the movable block (1001), and the rubber ring (1002) is fit-fitted and connected with the groove wall of the first limiting groove (102).

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