Culture solution and culture method for ganoderma lucidum

Abstract

The invention discloses a ganoderma lucidum culture solution and a culture method thereof. Comprising the following raw materials in parts: 30-40 parts of water, 10-20 parts of corn flour, 1-5 parts of ammonium nitrate, 1-5 parts of monopotassium phosphate, 1-5 parts of potassium sulfate, 1-5 parts of boric acid, 1-5 parts of manganese sulfate, 1-5 parts of zinc sulfate, 1-5 parts of copper sulfate, 1-5 parts of ferric sulfate, 1-5 parts of a short-acting growth regulator, 1-5 parts of amino acid, 1-5 parts of vitamins, 1-5 parts of a seaweed extract, 1-5 parts of honey and 1-5 parts of peaches and oranges. The short-acting growth regulator is prepared from gibberellin, auxin and cytokinin according to the ratio of (2-5): (1-8): (2-9), and the amino acid is prepared by mixing isoleucine, lysine, methionine and phenylalanine according to the ratio of (1-5): (2-8): (4-9): (5-7). The nutrient purity of the culture solution can be greatly increased, the fatality rate is reduced, cell elongation and rooting are promoted, plant metabolism and growth can be greatly promoted, and the quality of ganoderma lucidum is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of culturing medium for culturing cyperus, and in particular to a culturing medium for culturing cyperus and a culturing method thereof. Background Art

[0002] Tai Sui, also known as "Meat Ganoderma", grows underground and is a rare aggregate composed of three types of fungi: slime molds, bacteria and fungi. It is a specific fungus and a complex of multiple organisms. It usually grows in a humid environment. Tai Sui fungus usually has a block-like or gelatinous structure, a smooth surface, and is mostly gray or light yellow in color. It is rich in various amino acids, trace elements and polysaccharides, and has certain nutritional value. It is widely used in traditional Chinese medicine and folk remedies, and is believed to have the effects of nourishing qi and blood, and strengthening the body.

[0003] Tai Sui Mushroom is suitable for growing in a humid and warm environment. It usually requires a dark and moist substrate and a culture solution to provide the necessary nutrients for the growth of Tai Sui Mushroom.

[0004] However, most of the culturing solutions in the prior art are extracts prepared from chemical fertilizers, and the nutrient solutions have low purity or contain harmful substances. Long-term use may cause the death of cultivars and the effect is not good. Therefore, the present invention proposes a culturing solution and a culturing method thereof to solve the above problems. Summary of the invention

[0005] Based on the technical problem that most of the culture medium of Ganoderma lucidum is an extract prepared from chemical fertilizers, the nutrient solution has low purity, or contains harmful substances, and long-term use will cause the death of Ganoderma lucidum and the effect is not good, the present invention proposes a culture medium of Ganoderma lucidum and a culture method thereof.

[0006] The present invention provides a culture solution for Ganoderma lucidum, which comprises the following raw materials in parts: 30-40 parts of water, 10-20 parts of corn flour, 1-5 parts of ammonium nitrate, 1-5 parts of potassium dihydrogen phosphate, 1-5 parts of potassium sulfate, 1-5 parts of boric acid, 1-5 parts of manganese sulfate, 1-5 parts of zinc sulfate, 1-5 parts of copper sulfate, 1-5 parts of iron sulfate, 1-5 parts of short-term growth regulator, 1-5 parts of amino acid, 1-5 parts of vitamin, 1-5 parts of seaweed extract, 1-5 parts of honey, and 1-5 parts of peach and orange. Ammonium nitrate is an ammonium salt with the chemical formula of NH4NO3 and is white in color. Crystalline powder, easily soluble in water, easy to absorb moisture and agglomerate, adding nitrogen to the nutrient solution, potassium dihydrogen phosphate adding phosphorus to the nutrient solution, agricultural potassium sulfate is mostly light yellow in appearance, potassium sulfate has low hygroscopicity, is not easy to agglomerate, has good physical properties, and is easy to apply. It is a very good water-soluble potash fertilizer and the main raw material for making chlorine-free nitrogen, phosphorus, and potassium ternary compound fertilizers. Boric acid, manganese sulfate, zinc sulfate, copper sulfate, and iron sulfate are added to the nutrient solution as trace elements. Plant growth regulators are artificially synthesized (or natural extracted from microorganisms) and have a regulating effect on plant growth and development. Chemical substances with similar chemical properties to plant hormones. Amino acids can provide basic nutrients required for cell growth and proliferation, provide raw materials for cells to synthesize their own proteins, support cell functions, affect cell metabolic pathways, promote cell health and growth, enhance cells' ability to adapt to adverse environments, and improve survival rates. Vitamin B group helps cell division and growth. Vitamins, as cofactors of enzymes, participate in the metabolic process of plants and improve energy conversion efficiency. Certain vitamins can enhance plants' resistance to environmental stress and improve survival rates. By promoting physiological processes, they improve the overall growth of plants, thereby increasing yield and quality. Seaweed extracts contain plant growth hormones, such as auxin and cytokinin, which can promote plant growth and development. Seaweed extracts can promote root growth and improve the root system's ability to absorb water and nutrients. Seaweed extracts help enhance plants' resistance to environmental stress (such as drought, disease and salinity). Seaweed extracts can improve plants' absorption of nutrients and promote the effective use of nutrients. The organic matter in seaweed extracts can promote the activity of soil microorganisms, thereby improving soil quality.

[0007] Preferably, the short-acting growth regulator is gibberellin, auxin and cytokinin, and the ratio thereof is 2-5:1-8:2-9, and the amino acid is a mixture of isoleucine, lysine, methionine and phenylalanine, and the ratio thereof is 1-5:2-8:4-9:5-7.

[0008] Preferably, the vitamins specifically include: vitamin B6, vitamin B12, folic acid and vitamin D.

[0009] Preferably, the seaweed extract is specifically extracted from blue algae and green algae, and the ratio thereof is 1:5.

[0010] The present invention also provides a method for culturing the culturing fluid of the culturing fluid, comprising the following steps:

[0011] S1: Preparation of culture medium;

[0012] S2: Sterilization;

[0013] S3: inoculation;

[0014] S4: Closed

[0015] S5: Growth conditions;

[0016] S6: Observation and harvest.

[0017] Preferably, in the step S1, water and corn flour are put into the inner side of a blender for mixing, the blender parameters are 120-150 revolutions per minute, the temperature is 50-60 degrees Celsius, and after stirring for 10-15 minutes, short-acting growth regulators, amino acids, vitamins, seaweed extracts, honey, peaches and oranges are added to the inner side of the blender and stirring is continued, and the speed is increased to 150-180 revolutions per minute. After 10-15 minutes, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate and iron sulfate are added, and stirring is continued for 20-25 minutes to obtain the Tai Sui culture solution.

[0018] Preferably, in step S2, after the preparation of the Ganoderma lucidum culture solution is completed, the Ganoderma lucidum culture solution is placed in a pressure steam sterilizer with a temperature set at 90-120 degrees for 10-20 minutes.

[0019] Preferably, in the step S3, the Ganoderma lucidum strain is inoculated into the Ganoderma lucidum culture solution in a sterile environment, and the ratio of Ganoderma lucidum to the culture solution is 1:8.

[0020] Preferably, in the step S4, the container containing the ginseng is sealed with a plastic film to keep it moist.

[0021] Preferably, in steps S5 and S6, the ambient temperature of the cultivar is set at 20-25 degrees Celsius, and the humidity is maintained at 80-95 percent, direct sunlight is avoided, a scattered light environment can be selected, water mist is sprayed once every 1-2 days to keep it moist, mycelium growth and environment are checked regularly, and it can be harvested after 2-4 weeks.

[0022] Beneficial effects of the present invention:

[0023] The present invention can greatly increase the nutritional purity of the culture solution, reduce the mortality rate, promote cell elongation and rooting, greatly promote plant metabolism and growth, and improve the quality of the cultivar by adding various trace elements and additives into the common cultivar culture solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The working flow chart proposed by the present invention. DETAILED DESCRIPTION

[0025] The present invention will be further explained below in conjunction with specific embodiments.

[0026] Reference Figure 1 , Embodiment 1

[0027] This embodiment provides a Cnidium monnieri culture solution, comprising the following raw materials:

[0028] 30 parts of water, 20 parts of corn flour, 2 parts of ammonium nitrate, 5 parts of potassium dihydrogen phosphate, 3 parts of potassium sulfate, 3 parts of boric acid, 3 parts of manganese sulfate, 5 parts of zinc sulfate, 3 parts of copper sulfate, 2 parts of iron sulfate, 3 parts of short-term growth regulator, 5 parts of amino acid, 4 parts of vitamins, 5 parts of seaweed extract, 4 parts of honey, 3 parts of peaches and oranges, wherein the short-term growth regulator is gibberellin, auxin and cytokinin, and the ratio is 2:1:2; the amino acid is a mixture of isoleucine, lysine, methionine and phenylalanine, and the ratio is 1:2:4:5; the vitamins specifically include: vitamin B6, vitamin B12, folic acid and vitamin Element D, the seaweed extract is specifically extracted from blue algae and green algae, and the ratio is 1:5. Ammonium nitrate is an ammonium salt with a chemical formula of NH4NO3. It is a white crystalline powder, very soluble in water, easy to absorb moisture and agglomerate. It is used to add nitrogen to the nutrient solution. Potassium dihydrogen phosphate is used to add phosphorus to the nutrient solution. Agricultural potassium sulfate is mostly light yellow in appearance. Potassium sulfate has low hygroscopicity, is not easy to agglomerate, has good physical properties, is easy to apply, is a good water-soluble potash fertilizer, and is also the main raw material for making chlorine-free nitrogen, phosphorus, and potassium ternary compound fertilizers. Boric acid, manganese sulfate, zinc sulfate, copper sulfate, and iron sulfate are added to the nutrient solution as trace elements. Plant growth regulators are Artificially synthesized (or natural extracted from microorganisms), chemical substances that regulate the growth and development of plants, with chemical properties similar to plant hormones. Amino acids can provide the basic nutrients required for cell growth and proliferation, provide raw materials for cells to synthesize their own proteins, support cell functions, affect cell metabolic pathways, promote cell health and growth, enhance cells' ability to adapt to adverse environments, and improve survival rates. Vitamin B group helps cell division and growth. Vitamins, as cofactors of enzymes, participate in the metabolic process of plants and improve energy conversion efficiency. Certain vitamins can enhance plants' resistance to environmental stress and improve survival rates. By promoting physiological processes, they improve the overall growth of plants, thereby increasing yield and quality. Seaweed extracts contain plant growth hormones, such as auxin and cytokinin, which can promote plant growth and development. Seaweed extracts can promote root growth and improve the root's ability to absorb water and nutrients. Seaweed extracts help enhance plants' resistance to environmental stress (such as drought, disease and salinity). Seaweed extracts can improve plants' absorption of nutrients and promote the effective use of nutrients. Organic matter in seaweed extracts can promote the activity of soil microorganisms, thereby improving soil quality.

[0029] The present invention also provides a method for culturing the culturing fluid of the culturing fluid, comprising the following steps:

[0030] S1: Preparation of culture solution: water and corn flour are put into a blender for mixing at 120 rpm and 50 degrees Celsius. After 10 minutes of stirring, short-acting growth regulators, amino acids, vitamins, seaweed extracts, honey, peaches and oranges are added into the blender and stirred. The speed is increased to 150 rpm. After 10 minutes, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate and iron sulfate are added. Stirring is continued for 20 minutes to obtain the Tai Sui culture solution.

[0031] S2: Sterilization: After the preparation of the culture medium of Ganoderma lucidum is completed, the culture medium of Ganoderma lucidum is placed in a pressure steam sterilizer, the temperature of which is set to 90 degrees, and the time is 10 minutes;

[0032] S3: Inoculation, inoculate the cultivar of Gastrodia elata into the Gastrodia elata culture medium in a sterile environment, with the ratio of Gastrodia elata to culture medium being 1:8;

[0033] S4: Seal, seal the container containing Tai Sui with plastic film to keep it moist;

[0034] S5: Growth conditions: set the temperature around the plant to 20 degrees Celsius and keep the humidity at 80 percent. Avoid direct sunlight. You can choose a diffuse light environment. Spray water mist once a day to keep it moist.

[0035] S6: Observation and harvesting. Check the mycelium growth and environment regularly. Harvesting can be carried out after 3 weeks.

[0036] Reference Figure 1 , Embodiment 2

[0037] This embodiment provides a Cnidium monnieri culture solution, comprising the following raw materials:

[0038] 35 parts of water, 20 parts of corn flour, 2 parts of ammonium nitrate, 5 parts of potassium dihydrogen phosphate, 5 parts of potassium sulfate, 5 parts of boric acid, 3 parts of manganese sulfate, 1 part of zinc sulfate, 2 parts of copper sulfate, 4 parts of iron sulfate, 1 part of short-term growth regulator, 3 parts of amino acids, 2 parts of vitamins, 5 parts of seaweed extract, 4 parts of honey, 3 parts of peaches and oranges, wherein the short-term growth regulator is gibberellin, auxin and cytokinin, and the ratio is 3:5:3; the amino acid is a mixture of isoleucine, lysine, methionine and phenylalanine, and the ratio is 2:3:2:3; the vitamins specifically include: vitamin B6, vitamin B12, folic acid and vitamin Element D, the seaweed extract is specifically extracted from blue algae and green algae, and the ratio is 1:5. Ammonium nitrate is an ammonium salt with a chemical formula of NH4NO3. It is a white crystalline powder, very soluble in water, easy to absorb moisture and agglomerate. It is used to add nitrogen to the nutrient solution. Potassium dihydrogen phosphate is used to add phosphorus to the nutrient solution. Agricultural potassium sulfate is mostly light yellow in appearance. Potassium sulfate has low hygroscopicity, is not easy to agglomerate, has good physical properties, is easy to apply, is a good water-soluble potash fertilizer, and is also the main raw material for making chlorine-free nitrogen, phosphorus, and potassium ternary compound fertilizers. Boric acid, manganese sulfate, zinc sulfate, copper sulfate, and iron sulfate are added to the nutrient solution as trace elements. Plant growth regulators are Artificially synthesized (or natural extracted from microorganisms), chemical substances that regulate the growth and development of plants, with chemical properties similar to plant hormones. Amino acids can provide the basic nutrients required for cell growth and proliferation, provide raw materials for cells to synthesize their own proteins, support cell functions, affect cell metabolic pathways, promote cell health and growth, enhance cells' ability to adapt to adverse environments, and improve survival rates. Vitamin B group helps cell division and growth. Vitamins, as cofactors of enzymes, participate in the metabolic process of plants and improve energy conversion efficiency. Certain vitamins can enhance plants' resistance to environmental stress and improve survival rates. By promoting physiological processes, they improve the overall growth of plants, thereby increasing yield and quality. Seaweed extracts contain plant growth hormones, such as auxin and cytokinin, which can promote plant growth and development. Seaweed extracts can promote root growth and improve the root's ability to absorb water and nutrients. Seaweed extracts help enhance plants' resistance to environmental stress (such as drought, disease and salinity). Seaweed extracts can improve plants' absorption of nutrients and promote the effective use of nutrients. Organic matter in seaweed extracts can promote the activity of soil microorganisms, thereby improving soil quality.

[0039] The present invention also provides a method for culturing the culturing fluid of the culturing fluid, comprising the following steps:

[0040] S1: Preparation of culture solution: water and corn flour are put into the inner side of a blender for mixing; the blender parameters are 134 revolutions per minute and the temperature is 55 degrees Celsius; after stirring for 12 minutes, short-acting growth regulators, amino acids, vitamins, seaweed extracts, honey, peaches and oranges are added into the inner side of the blender and continued to stir; the speed is increased to 162 revolutions per minute; after 12 minutes, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate and iron sulfate are added; stirring is continued for 22 minutes to obtain the Tai Sui culture solution;

[0041] S2: Sterilization. After the preparation of the culture medium of Ganoderma lucidum is completed, the culture medium of Ganoderma lucidum is placed in a pressure steam sterilizer with a temperature set to 100 degrees and a time of 17 minutes;

[0042] S3: Inoculation, inoculate the cultivar of Gastrodia elata into the Gastrodia elata culture medium in a sterile environment, with the ratio of Gastrodia elata to culture medium being 1:8;

[0043] S4: Seal, seal the container containing Tai Sui with plastic film to keep it moist;

[0044] S5: Growth conditions: set the temperature around the plant to 25 degrees Celsius and keep the humidity at 90 percent. Avoid direct sunlight. You can choose a diffuse light environment. Spray water mist every two days to keep it moist.

[0045] S6: Observation and harvesting. Check the mycelium growth and environment regularly. Harvesting can be carried out after 4 weeks.

[0046] Reference Figure 1 , Embodiment 3

[0047] This embodiment provides a Cnidium monnieri culture solution, comprising the following raw materials:

[0048] 36 parts of water, 17 parts of corn flour, 2 parts of ammonium nitrate, 3 parts of potassium dihydrogen phosphate, 5 parts of potassium sulfate, 4 parts of boric acid, 5 parts of manganese sulfate, 2 parts of zinc sulfate, 2 parts of copper sulfate, 3 parts of iron sulfate, 1 part of short-term growth regulator, 4 parts of amino acids, 3 parts of vitamins, 4 parts of seaweed extract, 3 parts of honey, 5 parts of peaches and oranges, wherein the short-term growth regulator is gibberellin, auxin and cytokinin, and the ratio is 4:5:7; the amino acid is a mixture of isoleucine, lysine, methionine and phenylalanine, and the ratio is 3:6:5:6; the vitamins specifically include: vitamin B6, vitamin B12, folic acid and vitamin Element D, the seaweed extract is specifically extracted from blue algae and green algae, and the ratio is 1:5. Ammonium nitrate is an ammonium salt with a chemical formula of NH4NO3. It is a white crystalline powder, very soluble in water, easy to absorb moisture and agglomerate. It is used to add nitrogen to the nutrient solution. Potassium dihydrogen phosphate is used to add phosphorus to the nutrient solution. Agricultural potassium sulfate is mostly light yellow in appearance. Potassium sulfate has low hygroscopicity, is not easy to agglomerate, has good physical properties, is easy to apply, is a good water-soluble potash fertilizer, and is also the main raw material for making chlorine-free nitrogen, phosphorus, and potassium ternary compound fertilizers. Boric acid, manganese sulfate, zinc sulfate, copper sulfate, and iron sulfate are added to the nutrient solution as trace elements. Plant growth regulators are Artificially synthesized (or natural extracted from microorganisms), chemical substances that regulate the growth and development of plants, with chemical properties similar to plant hormones. Amino acids can provide the basic nutrients required for cell growth and proliferation, provide raw materials for cells to synthesize their own proteins, support cell functions, affect cell metabolic pathways, promote cell health and growth, enhance cells' ability to adapt to adverse environments, and improve survival rates. Vitamin B group helps cell division and growth. Vitamins, as cofactors of enzymes, participate in the metabolic process of plants and improve energy conversion efficiency. Certain vitamins can enhance plants' resistance to environmental stress and improve survival rates. By promoting physiological processes, they improve the overall growth of plants, thereby increasing yield and quality. Seaweed extracts contain plant growth hormones, such as auxin and cytokinin, which can promote plant growth and development. Seaweed extracts can promote root growth and improve the root's ability to absorb water and nutrients. Seaweed extracts help enhance plants' resistance to environmental stress (such as drought, disease and salinity). Seaweed extracts can improve plants' absorption of nutrients and promote the effective use of nutrients. Organic matter in seaweed extracts can promote the activity of soil microorganisms, thereby improving soil quality.

[0049] The present invention also provides a method for culturing the culturing fluid of the culturing fluid, comprising the following steps:

[0050] S1: Preparation of culture medium: water and corn flour are put into the inner side of a blender for mixing; the blender parameters are 135 revolutions per minute and the temperature is 56 degrees Celsius; after stirring for 12 minutes, short-acting growth regulators, amino acids, vitamins, seaweed extracts, honey, peaches and oranges are added into the inner side of the blender and continued to stir; the speed is increased to 170 revolutions per minute; after 13 minutes, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate and iron sulfate are added; stirring is continued for 23 minutes to obtain the Tai Sui culture medium;

[0051] S2: Sterilization: After the preparation of the culture medium of Ganoderma lucidum, the culture medium of Ganoderma lucidum is placed in a pressure steam sterilizer, the temperature of which is set to 110 degrees, and the time is 15 minutes;

[0052] S3: Inoculation, inoculate the cultivar of Gastrodia elata into the Gastrodia elata culture medium in a sterile environment, with the ratio of Gastrodia elata to culture medium being 1:8;

[0053] S4: Seal, seal the container containing Tai Sui with plastic film to keep it moist;

[0054] S5: Growth conditions: set the temperature around the plant to 23 degrees Celsius and keep the humidity at 80-95 percent. Avoid direct sunlight. You can choose a diffuse light environment. Spray water mist every two days to keep it moist.

[0055] S6: Observation and harvesting. Check the mycelium growth and environment regularly. Harvesting can be carried out after 3 weeks.

[0056] Reference Figure 1 , Embodiment 4

[0057] This embodiment provides a Cnidium monnieri culture solution, comprising the following raw materials:

[0058] 37 parts of water, 16 parts of corn flour, 3 parts of ammonium nitrate, 5 parts of potassium dihydrogen phosphate, 4 parts of potassium sulfate, 3 parts of boric acid, 2 parts of manganese sulfate, 2 parts of zinc sulfate, 4 parts of copper sulfate, 3 parts of iron sulfate, 4 parts of short-term growth regulator, 5 parts of amino acid, 3 parts of vitamins, 3 parts of seaweed extract, 2 parts of honey, 4 parts of peaches and oranges, wherein the short-term growth regulator is gibberellin, auxin and cytokinin, and the ratio is 3:7:5; the amino acid is a mixture of isoleucine, lysine, methionine and phenylalanine, and the ratio is 3:4:5:6; the vitamins specifically include: vitamin B6, vitamin B12, folic acid and vitamin Element D, the seaweed extract is specifically extracted from blue algae and green algae, and the ratio is 1:5. Ammonium nitrate is an ammonium salt with a chemical formula of NH4NO3. It is a white crystalline powder, very soluble in water, easy to absorb moisture and agglomerate. It is used to add nitrogen to the nutrient solution. Potassium dihydrogen phosphate is used to add phosphorus to the nutrient solution. Agricultural potassium sulfate is mostly light yellow in appearance. Potassium sulfate has low hygroscopicity, is not easy to agglomerate, has good physical properties, is easy to apply, is a good water-soluble potash fertilizer, and is also the main raw material for making chlorine-free nitrogen, phosphorus, and potassium ternary compound fertilizers. Boric acid, manganese sulfate, zinc sulfate, copper sulfate, and iron sulfate are added to the nutrient solution as trace elements. Plant growth regulators are Artificially synthesized (or natural extracted from microorganisms), chemical substances that regulate the growth and development of plants, with chemical properties similar to plant hormones. Amino acids can provide the basic nutrients required for cell growth and proliferation, provide raw materials for cells to synthesize their own proteins, support cell functions, affect cell metabolic pathways, promote cell health and growth, enhance cells' ability to adapt to adverse environments, and improve survival rates. Vitamin B group helps cell division and growth. Vitamins, as cofactors of enzymes, participate in the metabolic process of plants and improve energy conversion efficiency. Certain vitamins can enhance plants' resistance to environmental stress and improve survival rates. By promoting physiological processes, they improve the overall growth of plants, thereby increasing yield and quality. Seaweed extracts contain plant growth hormones, such as auxin and cytokinin, which can promote plant growth and development. Seaweed extracts can promote root growth and improve the root's ability to absorb water and nutrients. Seaweed extracts help enhance plants' resistance to environmental stress (such as drought, disease and salinity). Seaweed extracts can improve plants' absorption of nutrients and promote the effective use of nutrients. Organic matter in seaweed extracts can promote the activity of soil microorganisms, thereby improving soil quality.

[0059] The present invention also provides a method for culturing the culturing fluid of the culturing fluid, comprising the following steps:

[0060] S1: Preparation of culture solution: water and corn flour are put into the inner side of a blender for mixing; the blender parameters are 145 revolutions per minute and the temperature is 58 degrees Celsius; after stirring for 13 minutes, short-acting growth regulators, amino acids, vitamins, seaweed extracts, honey, peaches and oranges are added into the inner side of the blender and continued to stir; the speed is increased to 170 revolutions per minute; after 12 minutes, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate and iron sulfate are added; stirring is continued for 23 minutes to obtain the Tai Sui culture solution;

[0061] S2: Sterilization. After the preparation of the culture medium of Ganoderma lucidum is completed, the culture medium of Ganoderma lucidum is placed in a pressure steam sterilizer with a temperature set to 100 degrees and a time of 15 minutes;

[0062] S3: Inoculation, inoculate the cultivar of Gastrodia elata into the Gastrodia elata culture medium in a sterile environment, with the ratio of Gastrodia elata to culture medium being 1:8;

[0063] S4: Seal, seal the container containing Tai Sui with plastic film to keep it moist;

[0064] S5: Growth conditions: Set the temperature around the plant to 24 degrees Celsius and keep the humidity at 80-95 percent. Avoid direct sunlight. You can choose a diffuse light environment. Spray water mist once a day to keep it moist.

[0065] S6: Observation and harvesting. Check the mycelium growth and environment regularly. Harvesting can be carried out after 3 weeks.

[0066] Reference Figure 1 Embodiment 5

[0067] This embodiment provides a Cnidium monnieri culture solution, comprising the following raw materials:

[0068] 38 parts of water, 16 parts of corn flour, 3 parts of ammonium nitrate, 4 parts of potassium dihydrogen phosphate, 5 parts of potassium sulfate, 2 parts of boric acid, 4 parts of manganese sulfate, 3 parts of zinc sulfate, 4 parts of copper sulfate, 3 parts of iron sulfate, 5 parts of short-term growth regulator, 5 parts of amino acid, 4 parts of vitamins, 3 parts of seaweed extract, 1 part of honey, 2 parts of peaches and oranges, wherein the short-term growth regulator is gibberellin, auxin and cytokinin, and the ratio is 5:8:9, the amino acid is a mixture of isoleucine, lysine, methionine and phenylalanine, and the ratio is 5:8:9:7, and the vitamins specifically include: vitamin B6, vitamin B12, folic acid and vitamin Element D, the seaweed extract is specifically extracted from blue algae and green algae, and the ratio is 1:5. Ammonium nitrate is an ammonium salt with a chemical formula of NH4NO3. It is a white crystalline powder, very soluble in water, easy to absorb moisture and agglomerate. It is used to add nitrogen to the nutrient solution. Potassium dihydrogen phosphate is used to add phosphorus to the nutrient solution. Agricultural potassium sulfate is mostly light yellow in appearance. Potassium sulfate has low hygroscopicity, is not easy to agglomerate, has good physical properties, is easy to apply, is a good water-soluble potash fertilizer, and is also the main raw material for making chlorine-free nitrogen, phosphorus, and potassium ternary compound fertilizers. Boric acid, manganese sulfate, zinc sulfate, copper sulfate, and iron sulfate are added to the nutrient solution as trace elements. Plant growth regulators are Artificially synthesized (or natural extracted from microorganisms), chemical substances that regulate the growth and development of plants, with chemical properties similar to plant hormones. Amino acids can provide the basic nutrients required for cell growth and proliferation, provide raw materials for cells to synthesize their own proteins, support cell functions, affect cell metabolic pathways, promote cell health and growth, enhance cells' ability to adapt to adverse environments, and improve survival rates. Vitamin B group helps cell division and growth. Vitamins, as cofactors of enzymes, participate in the metabolic process of plants and improve energy conversion efficiency. Certain vitamins can enhance plants' resistance to environmental stress and improve survival rates. By promoting physiological processes, they improve the overall growth of plants, thereby increasing yield and quality. Seaweed extracts contain plant growth hormones, such as auxin and cytokinin, which can promote plant growth and development. Seaweed extracts can promote root growth and improve the root's ability to absorb water and nutrients. Seaweed extracts help enhance plants' resistance to environmental stress (such as drought, disease and salinity). Seaweed extracts can improve plants' absorption of nutrients and promote the effective use of nutrients. Organic matter in seaweed extracts can promote the activity of soil microorganisms, thereby improving soil quality.

[0069] The present invention also provides a method for culturing the culturing fluid of the culturing fluid, comprising the following steps:

[0070] S1: Preparation of culture solution: water and corn flour are put into a blender for mixing at a speed of 150 rpm and a temperature of 60 degrees Celsius. After stirring for 15 minutes, short-acting growth regulators, amino acids, vitamins, seaweed extracts, honey, peaches and oranges are added into the blender and stirred continuously at a speed of 180 rpm. After 15 minutes, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate and iron sulfate are added. Stirring is continued for 20-25 minutes to obtain Tai Sui culture solution.

[0071] S2: Sterilization: After the preparation of the culture medium of Ganoderma lucidum, the culture medium of Ganoderma lucidum is placed in a pressure steam sterilizer, the temperature of which is set to 120 degrees, and the time is 20 minutes;

[0072] S3: Inoculation, inoculate the cultivar of Gastrodia elata into the Gastrodia elata culture medium in a sterile environment, with the ratio of Gastrodia elata to culture medium being 1:8;

[0073] S4: Seal, seal the container containing Tai Sui with plastic film to keep it moist;

[0074] S5: Growth conditions: set the temperature around the plant to 25 degrees Celsius and keep the humidity at 95 percent. Avoid direct sunlight. You can choose a diffuse light environment. Spray water mist every two days to keep it moist.

[0075] S6: Observation and harvesting. Check the mycelium growth and environment regularly. Harvesting can be carried out after 4 weeks.

[0076] Comparing the conventional Ganoderma lucidum culture solution with the Ganoderma lucidum culture solution prepared in Examples 1 to 5, the results prepared in Examples 1 to 5 are shown in the following table:

[0077]

[0078] It can be seen from the above table that the culture medium and culture method of C. sui proposed in the present invention have obvious improvements, and implementation five is the best embodiment.

[0079] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A culture medium of Cnidium monnieri, characterized in that: The raw materials include the following parts: 30-40 parts of water, 10-20 parts of corn flour, 1-5 parts of ammonium nitrate, 1-5 parts of potassium dihydrogen phosphate, 1-5 parts of potassium sulfate, 1-5 parts of boric acid, 1-5 parts of manganese sulfate, 1-5 parts of zinc sulfate, 1-5 parts of copper sulfate, 1-5 parts of iron sulfate, 1-5 parts of short-acting growth regulator, 1-5 parts of amino acid, 1-5 parts of vitamins, 1-5 parts of seaweed extract, 1-5 parts of honey, and 1-5 parts of peaches and oranges.

2. A Cnidium monnieri culture solution according to claim 1, characterized in that: The short-acting growth regulator is gibberellin, auxin and cytokinin, and the ratio thereof is 2-5:1-8:2-9. The amino acid is prepared by mixing isoleucine, lysine, methionine and phenylalanine, and the ratio thereof is 1-5:2-8:4-9:5-7.

3. A Cnidium monnieri culture solution according to claim 1, characterized in that: The vitamins specifically include: vitamin B6, vitamin B12, folic acid and vitamin D.

4. The culture solution of Cnidium monnieri according to claim 1, characterized in that: The seaweed extract is specifically extracted from blue algae and green algae, and the ratio thereof is 1:

5.

5. A method for culturing Cnidium monnieri culture fluid, characterized in that: The following steps are involved: ； S1: Preparation of culture medium; S2: Sterilization; S3: inoculation; S4: Closed S5: Growth conditions; S6: Observation and harvest.

6. The method for culturing a Cnidium monnieri culture solution according to claim 5, characterized in that: In the step S1, water and corn flour are put into the inner side of a blender for mixing. The blender parameters are 120-150 revolutions per minute and the temperature is 50-60 degrees Celsius. After stirring for 10-15 minutes, short-acting growth regulators, amino acids, vitamins, seaweed extracts, honey, peaches and oranges are added to the inner side of the blender and stirring is continued. The speed is increased to 150-180 revolutions per minute. After 10-15 minutes, ammonium nitrate, potassium dihydrogen phosphate, potassium sulfate, boric acid, manganese sulfate, zinc sulfate, copper sulfate and iron sulfate are added. Stirring is continued for 20-25 minutes to obtain a Tai Sui culture solution.

7. The method for culturing a Cnidium monnieri culture solution according to claim 5, characterized in that: In the step S2, after the preparation of the Ganoderma lucidum culture solution is completed, the Ganoderma lucidum culture solution is placed in a pressure steam sterilizer with a temperature set at 90-120 degrees for 10-20 minutes.

8. The method for culturing a Cnidium monnieri culture solution according to claim 5, characterized in that: In the step S3, the cultivar of Gastrodia elata is inoculated into the Gastrodia elata culture solution in a sterile environment, and the ratio of Gastrodia elata to the culture solution is 1:

8.

9. The method for culturing a Cnidium monnieri culture solution according to claim 5, characterized in that: In the step S4, the container containing the ginseng is sealed with a plastic film to keep it moist.

10. The method for culturing Cnidium monnieri culture solution according to claim 5, characterized in that: In the steps S5 and S6, the ambient temperature of the cultivar is set at 20-25 degrees Celsius, and the humidity is maintained at 80-95 percent, avoiding direct sunlight, and a scattered light environment can be selected. Water mist is sprayed once every 1-2 days to keep it moist, and the mycelium growth and environment are checked regularly. The cultivar can be harvested after 2-4 weeks.

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