Preparation method of sea-buckthorn instant tea

Abstract

The invention relates to the technical field of instant tea, and particularly discloses a preparation method of sea-buckthorn instant tea. A preparation method of sea-buckthorn instant tea comprises the following steps: 1) cleaning sea-buckthorn leaves, selecting, removing impurities, mashing into powder, sieving, and drying to obtain sea-buckthorn leaf powder; (2) uniformly mixing an alcohol solvent and the hippophae rhamnoides leaf powder, standing, heating and extracting, and carrying out solid-liquid separation to obtain a leaching solution; (3) uniformly mixing the leach liquor and deionized water to prepare prepad fluid, then adding calcium chloride and organic amine into the prepad fluid, stirring to completely dissolve the calcium chloride, and then introducing carbon dioxide to obtain intermediate fluid; 4) adding modified cellulose and water into the container, and uniformly mixing to obtain a base solution; then adding hydroxyproline into the intermediate solution, then slowly adding the base solution, and homogenizing to obtain a to-be-dried solution; and 5) heating and volatilizing the to-be-dried liquid to remove the solvent and the organic amine, and then freeze-drying to obtain the product. The prepared sea-buckthorn instant tea has the advantage of being good in dissolution effect.

Description

Technical Field

[0001] The invention relates to the technical field of instant tea, and more particularly to a method for preparing seabuckthorn instant tea. Background Art

[0002] Instant tea refers to an instant beverage made by extracting, concentrating and drying tea leaves. It has the advantages of fast brewing, convenience and no residue. Common instant teas include black tea, oolong tea, jasmine tea and green tea. Compared with traditional brewed tea, these instant teas can retain more active ingredients, such as polyphenols, catechins, amino acids, trace elements, etc., and have very good health benefits.

[0003] In addition to traditional instant teas, there are also many herbal instant teas with physiological functions, such as chrysanthemum, Sterculia lychnophora, sea buckthorn, gardenia, monk fruit and other instant teas. Among them, sea buckthorn leaves are rich in bioactive ingredients - bioflavonoids, which are strong antioxidants that can effectively remove free radicals in the body and delay cell degeneration and aging. In addition, bioflavonoids can also improve blood circulation and play a role in lowering cholesterol. This effect greatly reduces the incidence of cardiovascular and cerebrovascular diseases, has very good physiological activity, and is widely loved by the public.

[0004] Although the physiological effect of seabuckthorn instant tea is good, bioflavonoids are molecules with strong planarity, which are insoluble in water and soluble in organic solvents such as ethanol and ether. Therefore, the brewing effect of seabuckthorn instant tea is poor compared with other tea beverages, and the dissolution rate and stability have a lot of room for improvement. For example, the Chinese patent application with application publication number CN1240298A discloses a seabuckthorn leaf instant tea and a preparation method thereof, which is a seabuckthorn instant tea powder prepared by drying and modulating the water extract of seabuckthorn leaves, the drying process is freeze drying, and edible essence is added as an auxiliary material, but the dissolution rate of this instant seabuckthorn leaf tea is slow and precipitation is easy to occur.

[0005] For another example, a Chinese patent application with application publication number CN104663956A discloses a safflower sea buckthorn dynamic instant tea powder, which is made from safflower velvet and sea buckthorn processing residues as raw materials, and uses water as an extraction medium to extract the effective ingredients in safflower, and then refines them through purification and concentration; then ultrasound-assisted complex enzymes are used to extract the effective ingredients in the sea buckthorn branch and leaf residues, and the instant tea powder is made through vacuum freeze-drying and ultrafine grinding technology.

[0006] In the above documents, although ultrafine grinding technology is used to improve the instant dissolution effect, this method can only speed up the dissolution to a certain extent and is not of much help to the subsequent dispersion stability of the tea soup. Summary of the invention

[0007] In order to further improve the dissolution rate and dispersion stability of seabuckthorn instant tea, the present invention provides a preparation method of seabuckthorn instant tea.

[0008] The present invention provides a method for preparing seabuckthorn instant tea, which adopts the following technical scheme:

[0009] A method for preparing seabuckthorn instant tea comprises the following steps:

[0010] 1) Wash the seabuckthorn leaves, select and remove impurities, crush them into powder, sieve and dry them to obtain seabuckthorn leaf powder;

[0011] 2) mixing an alcohol solvent and seabuckthorn leaf powder evenly, letting it stand and then heating for extraction, and obtaining an extract after solid-liquid separation;

[0012] 3) taking the leaching solution and deionized water and mixing them evenly to obtain a pre-fluid, then adding calcium chloride and an organic amine into the pre-fluid, stirring to completely dissolve the calcium chloride, and then introducing carbon dioxide to obtain an intermediate solution;

[0013] 4) Add modified cellulose and water into a container and mix well to obtain a base liquid; then add hydroxyproline to the intermediate liquid, raise the temperature to 35-50° C., then slowly add the base liquid, and homogenize to obtain a liquid to be dried;

[0014] 5) The liquid to be dried is heated to evaporate and remove the solvent and organic amine, and then freeze-dried to obtain the product.

[0015] By adopting the above technical scheme, the present invention adopts the mixture of leaching liquid and deionized water as the pre-liquid, which can dissolve calcium chloride well. After passing through carbon dioxide, carbon dioxide gas produces carbonate ions in the pre-liquid, and a stable coordination system is formed between carbonate ions, calcium ions and organic amine molecules, thereby generating a nano-rod-shaped or nano-clustered calcium carbonate dispersion, and a certain proportion of calcium carbonate dispersion can promote the dispersion of flavonoid components in the system, improve the dispersion stability, dissolution performance and compatibility of flavonoids and other insoluble and indispersible components. And after adding a base liquid with modified cellulose as a basic stabilizing material, when the modified cellulose is dissolved, the dissolution of flavonoids and other insoluble and indispersible components can be effectively increased, and the dispersion stability is improved. And the calcium carbonate dispersion formed by the present invention is nano-level, which will not have an adverse effect on the color and transparency of tea soup.

[0016] In addition, after the base liquid and the intermediate liquid are mixed, these calcium carbonate dispersions are evenly distributed in the system. With the assistance of hydroxyproline, the calcium carbonate dispersions are adsorbed on the micro-network structure formed by the modified cellulose, which can further enhance the anti-aggregation performance of the network structure, slow down the interference of water in the flavonoid dispersion system, and greatly enhance the dispersion stability of flavonoids and other insoluble and difficult to disperse components in the system, thereby improving the dissolution rate and dissolution effect of instant tea as a whole.

[0017] Preferably, the step 3) further includes one or more of the following technical features:

[0018] 21) The mass proportion of the extract in the pre-liquid is 60-75%;

[0019] 22) The organic amine is one or more of dimethylamine, trimethylamine, diethylamine and triethylamine;

[0020] 23) The molar ratio of calcium chloride to organic amine is 1:20-30;

[0021] 24) The molar ratio of carbon dioxide to calcium chloride is (2.5-3):1;

[0022] 25) The mass ratio of pre-liquid to calcium chloride is 1:(0.005-0.01).

[0023] By adopting the above technical scheme, optimizing and screening the mass proportion of the extract in the pre-liquid, a stable and uniform coordination system can be formed, and the molar ratio of calcium chloride to organic amine is screened, so as to regulate the particle size of the nanorod-shaped or nanocluster-shaped calcium carbonate dispersion. The size of the calcium carbonate dispersion has a great influence on the stability of the suspension system, and the calcium carbonate dispersion of the appropriate size has better adsorption stability and adsorption distribution morphology on the micro-network structure. In addition, selecting different types of organic amines as coordination components and regulating the molar ratio of carbon dioxide to calcium carbonate are conducive to adjusting the particle size distribution of the calcium carbonate dispersion, reducing the proportion of calcium carbonate dispersions that are too small and too large, and further improving the dissolution effect of instant tea.

[0024] Preferably, in step 2), the alcohol solvent is a low dielectric constant alcohol solvent.

[0025] More preferably, the low dielectric constant alcohol solvent is ethanol or propanol.

[0026] By adopting the above technical scheme, the types and compositions of low dielectric constant alcohol solvents are optimized and screened, and the stability of the coordination system of carbonate ions, calcium ions and organic amine molecules is further improved, which is conducive to the formation of a calcium carbonate dispersion with a narrower particle size distribution range.

[0027] Preferably, the step 4) further includes one or more of the following technical features:

[0028] 41) The mass ratio of the base liquid, hydroxyproline and the intermediate liquid is (0.15-0.2):(0.03-0.05):1;

[0029] 42) Slowly adding the intermediate solution means first adding 30-50% of the total volume of the intermediate solution at a rate of 0.5-1 mL / min, and then adding the remaining intermediate solution at a rate of 2-3.5 mL / min;

[0030] 43) After slowly adding the intermediate solution, immediately place it in a cold trap to cool to 2-5°C.

[0031] By adopting the above technical solution, on the one hand, the ratio between the base liquid, hydroxyproline and the intermediate liquid is optimized, so that the amount of calcium carbonate dispersion in the dispersion system is at an appropriate level, which will not affect the subsequent drinking and tea quality. On the other hand, the addition speed of the intermediate liquid is controlled, so that the concentration of the calcium carbonate dispersion in the early stage of the base liquid increases slowly, which plays a better buffering role, and then the concentration of the calcium carbonate dispersion is increased at a faster rate. In addition, the subsequent cold trap rapid cooling effect can make a part of the dispersion system that has not formed a coordination system undergo a solid phase change, forming a part of new calcium carbonate dispersion, which plays an anchoring role in the dispersion state of flavonoids and other insoluble and difficult to disperse components, and has better stability and dispersibility.

[0032] Preferably, in step 4), the modified cellulose is one or more of sodium carboxymethyl cellulose, sodium carboxyethyl cellulose, and hydroxypropyl methyl cellulose.

[0033] Preferably, in step 4), the mass proportion of modified cellulose in the base liquid is 3-5%.

[0034] By adopting the above technical solution, optimizing and adjusting the type and composition of modified cellulose, a dispersed system with a more stable micro-network structure can be constructed. In addition, adjusting the mass proportion of modified cellulose in the base liquid can form a more stable and comprehensive solubilization effect on bioactive components such as flavonoids, reduce the migration and merging of water and other components in the solution, and other destabilizing effects, thereby obtaining better system stability, dispersion performance and dissolution performance in the subsequent brewing process.

[0035] Preferably, in step 4), a sorbitol preservative is also added during the homogenization process.

[0036] Preferably, in step 4), a sweetener is added during the homogenization process, and the sweetener is one or more of sucrose, erythritol, glucose, acesulfame potassium, and aspartame.

[0037] By adopting the above technical solution, traditional preservatives have certain toxicity and will cause certain damage to the human body. Sorbitol preservatives are safer preservatives. After entering the human body, they are absorbed and can be quickly metabolized into carbon dioxide and water in a short time. Therefore, they will not accumulate in the human body and produce toxicity. They are very friendly to the human body and suitable for special groups such as children.

[0038] In addition, sweeteners can be added to mask the bitterness, astringency and other unacceptable tastes of the relevant active ingredients in the seabuckthorn leaves, greatly improving the palatability of instant tea and making it easier for children and other groups to accept it. Moreover, after the calcium carbonate dispersion is introduced into the instant tea of ​​the present invention, it is made into a composite instant tea powder with a series of components such as sweeteners and preservatives. With the help of the nano-scale calcium carbonate dispersion, the compatibility between the components is better, and it is not easy to have instability such as precipitation and flocculation.

[0039] Preferably, in step 4), the homogenization is carried out at a speed of 1500-2000 rpm.

[0040] By adopting the above technical solution, screening and testing the appropriate homogenization speed, the synergistic system formed between the modified cellulose and the calcium carbonate dispersion can be effectively protected from being destroyed, and the dispersibility, stability and dissolution and rapid dissolution effects can be further improved.

[0041] In summary, the present invention has the following beneficial effects:

[0042] 1. The present invention adopts modified cellulose to form a micro-network structure in a dispersion system to improve the stability of tea soup after brewing. And the micro-network structure formed by modified cellulose can greatly improve the stability and dispersibility of insoluble and difficult-to-disperse components such as flavonoids. In addition, in the coordination environment formed by leaching solution, water and organic amine, the carbonate ions produced by carbon dioxide and calcium chloride form a stable coordination, thereby forming a calcium carbonate dispersion in a rod-like or clustered state. These calcium carbonate dispersions are adsorbed and dispersed in the micro-network structure, which can play a good auxiliary stabilizing role between flavonoids and other insoluble and difficult-to-disperse components and other phase systems, so that the dispersion, stability and dissolution effect of flavonoids and other insoluble and difficult-to-disperse components are good, thereby improving the instant dissolution effect of instant tea and the quality of tea soup.

[0043] 2. The present invention further optimizes and adjusts the mass ratio of the base liquid, hydroxyproline and the intermediate liquid, and regulates the content of the calcium carbonate dispersion in the dispersion system. The appropriate content of the calcium carbonate dispersion and the micro-network structure formed by the modified cellulose play a synergistic stabilizing role, and produce a "micelle-like" encapsulation effect on flavonoids and other insoluble and difficult to disperse components, which can effectively improve the dispersibility and dissolution effect of flavonoids and other insoluble and difficult to disperse components.

[0044] 3. The present invention screens and tests the types of organic amines, the molar ratio of calcium carbonate to organic amines, the types of low dielectric constant alcohol solvents, and the types of sweeteners and preservatives, and optimizes the relevant process parameters in the preparation process of instant tea to further improve the stability, dispersion performance and instant effect of instant tea. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1It is a data chart of dissolution experiment of sea buckthorn instant tea of ​​Examples 1-3 of the present invention and Comparative Examples 1-3. DETAILED DESCRIPTION

[0046] The present invention is further described in detail below with reference to the embodiments.

[0047] Unless otherwise specified, the raw materials used in the examples and comparative examples of the present invention are all commercially available.

[0048] Example 1

[0049] The preparation method of seabuckthorn instant tea of ​​this embodiment comprises the following steps:

[0050] 1) Wash the seabuckthorn leaves, select and remove impurities, crush them into powder, pass through an 80-mesh sieve, place them in a drying oven, and dry them at 60° C. to obtain seabuckthorn leaf powder;

[0051] 2) Mix an alcohol solvent and seabuckthorn leaf powder in a mass ratio of 30:1, let stand and then heat to 45°C, extract for 30 minutes at 50W ultrasonic power, and separate the solid and liquid by vacuum filtration to obtain an extract;

[0052] 3) 75 g of the extract and 25 g of deionized water were mixed to obtain a pre-fluid, and then 0.5 g of calcium chloride and 88.5 g of an organic amine were added to the pre-fluid, stirred to completely dissolve the calcium chloride, and then 0.336 L of carbon dioxide was introduced at a rate of 150 mL / min to obtain an intermediate solution;

[0053] 4) adding 0.3 g of modified cellulose and 97 g of deionized water into a stirring kettle and mixing them evenly to obtain a base liquid; adding 100 g of the intermediate liquid into a stirring kettle equipped with a high-speed stirrer, and then adding 5 g of hydroxyproline and mixing them evenly, heating to 35° C., and then slowly adding 20 g of the base liquid at a speed of 1.5 mL / min under stirring conditions, and homogenizing at a speed of 1500 rpm to obtain a liquid to be dried;

[0054] 5) The liquid to be dried is heated to volatilize and remove the solvent and the organic amine, and deionized water is continuously added during the removal process until the residual amount of the alcohol solvent and the organic amine is detected to be less than 300 ppm, and then freeze-dried to obtain the product.

[0055] The alcohol solvent is propanol, the organic amine is trimethylamine, the purity of calcium chloride is 99.9%, and the modified cellulose is sodium carboxymethyl cellulose.

[0056] Example 2

[0057] The preparation method of seabuckthorn instant tea of ​​this embodiment comprises the following steps:

[0058] 1) Wash the seabuckthorn leaves, select and remove impurities, crush them into powder, pass through an 80-mesh sieve, place them in a drying oven, and dry them at 60° C. to obtain seabuckthorn leaf powder;

[0059] 2) Mix an alcohol solvent and seabuckthorn leaf powder in a mass ratio of 30:1, let stand and then heat to 45°C, extract for 30 minutes at 50W ultrasonic power, and separate the solid and liquid by vacuum filtration to obtain an extract;

[0060] 3) 60 g of the extract and 40 g of deionized water were mixed to obtain a pre-fluid, and then 1 g of calcium chloride and 203.8 g of an organic amine were added to the pre-fluid, stirred to completely dissolve the calcium chloride, and then 0.56 L of carbon dioxide was introduced at a rate of 150 mL / min to obtain an intermediate solution;

[0061] 4) adding 0.5 g of modified cellulose and 95 g of deionized water into a stirring kettle and mixing them evenly to obtain a base liquid; adding 100 g of the intermediate liquid into a stirring kettle equipped with a high-speed stirrer, and then adding 3 g of hydroxyproline and mixing them evenly, heating to 35° C., and then slowly adding 7.5 g of the base liquid at a speed of 1 mL / min under stirring conditions, and homogenizing at a speed of 2000 rpm to obtain a liquid to be dried, and adding a preservative and a sweetener during the homogenization process;

[0062] 5) The liquid to be dried is heated to volatilize and remove the solvent and the organic amine, and deionized water is continuously added during the removal process until the residual amount of the alcohol solvent and the organic amine is detected to be less than 300 ppm, and then freeze-dried to obtain the product.

[0063] The alcohol solvent is ethanol. The organic amine is triethylamine. The purity of calcium chloride is 99.9%. The preservative is sorbitol. The sweetener is sucrose. The modified cellulose is sodium carboxyethyl cellulose.

[0064] Example 3

[0065] The preparation method of seabuckthorn instant tea of ​​this embodiment comprises the following steps:

[0066] 1) Wash the seabuckthorn leaves, select and remove impurities, crush them into powder, pass through an 80-mesh sieve, place them in a drying oven, and dry them at 60° C. to obtain seabuckthorn leaf powder;

[0067] 2) Mix an alcohol solvent and seabuckthorn leaf powder in a mass ratio of 30:1, let stand and then heat to 45°C, extract for 30 minutes at 50W ultrasonic power, and separate the solid and liquid by vacuum filtration to obtain an extract;

[0068] 3) 70 g of the extract and 30 g of deionized water were mixed to obtain a pre-fluid, and then 1 g of calcium chloride and 227.5 g of an organic amine were added to the pre-fluid, stirred to completely dissolve the calcium chloride, and then 0.56 L of carbon dioxide was introduced at a rate of 150 mL / min to obtain an intermediate solution;

[0069] 4) adding 0.45 g of modified cellulose and 95.5 g of deionized water into a stirring kettle and mixing them evenly to obtain a base liquid; adding 100 g of the intermediate liquid into a stirring kettle equipped with a high-speed stirrer, and then adding 3 g of hydroxyproline and mixing them evenly, heating to 35° C., then slowly adding 4.5 g of the base liquid at a rate of 0.5 mL / min under stirring, and then slowly adding 10.5 g of the base liquid at a rate of 2 mL / min, and immediately placing the base liquid into a cold trap to cool to 2-5° C. after the addition of the base liquid is completed, keeping the temperature for 2 hours, and homogenizing at a speed of 1800 rpm to obtain a liquid to be dried, and adding a preservative and a sweetener during the homogenization process;

[0070] 5) The liquid to be dried is heated to volatilize and remove the solvent and the organic amine, and deionized water is continuously added during the removal process until the residual amount of the alcohol solvent and the organic amine is detected to be less than 300 ppm, and then freeze-dried to obtain the product.

[0071] The alcohol solvent is ethanol, the organic amine is triethylamine, the purity of calcium chloride is 99.9%, the preservative is sorbitol, the sweetener is erythritol, and the modified cellulose is sodium carboxyethyl cellulose.

[0072] Comparative Example 1

[0073] The preparation method of the seabuckthorn instant tea of ​​this comparative example comprises the following steps:

[0074] 1) Wash the seabuckthorn leaves, select and remove impurities, crush them into powder, pass through an 80-mesh sieve, place them in a drying oven, and dry them at 60° C. to obtain seabuckthorn leaf powder;

[0075] 2) Mix an alcohol solvent and seabuckthorn leaf powder in a mass ratio of 30:1, let stand and then heat to 45°C, extract for 30 minutes at 50W ultrasonic power, and separate the solid and liquid by vacuum filtration to obtain an extract;

[0076] 3) 75 g of the extract and 25 g of deionized water were mixed to obtain a pre-fluid, and then 5 g of calcium chloride and 88.5 g of an organic amine were added to the pre-fluid, stirred to completely dissolve the calcium chloride, and then 3.36 L of carbon dioxide was introduced at a rate of 150 mL / min to obtain an intermediate solution;

[0077] 4) Add 100 g of the intermediate solution into a stirring kettle equipped with a high-speed stirrer, then add 5 g of hydroxyproline and mix well, heat to 35° C., and homogenize at a speed of 1500 rpm to obtain a liquid to be dried;

[0078] 5) The liquid to be dried is heated to volatilize and remove the solvent and the organic amine, and deionized water is continuously added during the removal process until the residual amount of the alcohol solvent and the organic amine is detected to be less than 300 ppm, and then freeze-dried to obtain the product.

[0079] The alcohol solvent is propanol, the organic amine is trimethylamine, and the purity of calcium chloride is 99.9%.

[0080] Comparative Example 2

[0081] The preparation method of the seabuckthorn instant tea of ​​this comparative example comprises the following steps:

[0082] 1) Wash the seabuckthorn leaves, select and remove impurities, crush them into powder, pass through an 80-mesh sieve, place them in a drying oven, and dry them at 60° C. to obtain seabuckthorn leaf powder;

[0083] 2) Mix an alcohol solvent and seabuckthorn leaf powder in a mass ratio of 30:1, let stand and then heat to 45°C, extract for 30 minutes at 50W ultrasonic power, and separate the solid and liquid by vacuum filtration to obtain an extract;

[0084] 3) 75 g of the extract and 25 g of deionized water were mixed to obtain a pre-fluid;

[0085] 4) Add 100 g of the pre-liquid into a stirring kettle with a high-speed stirrer, then add 0.5 g of nano-calcium carbonate, and homogenize at a speed of 1500 rpm to obtain a liquid to be dried;

[0086] 5) The liquid to be dried is heated to volatilize and remove the solvent and the organic amine, and deionized water is continuously added during the removal process until the residual amount of the alcohol solvent and the organic amine is detected to be less than 300 ppm, and then freeze-dried to obtain the product.

[0087] The alcohol solvent is propanol and the modified cellulose is sodium carboxymethyl cellulose.

[0088] Comparative Example 3

[0089] The preparation method of the seabuckthorn instant tea of ​​this comparative example comprises the following steps:

[0090] 1) Wash the seabuckthorn leaves, select and remove impurities, crush them into powder, pass through an 80-mesh sieve, place them in a drying oven, and dry them at 60° C. to obtain seabuckthorn leaf powder;

[0091] 2) Mix an alcohol solvent and seabuckthorn leaf powder in a mass ratio of 30:1, let stand and then heat to 45°C, extract for 30 minutes at 50W ultrasonic power, and separate the solid and liquid by vacuum filtration to obtain an extract;

[0092] 3) 75 g of the extract and 25 g of deionized water were mixed to obtain a pre-fluid;

[0093] 4) adding 0.3 g of modified cellulose and 97 g of deionized water into a stirring kettle and mixing them evenly to obtain a base liquid; adding 100 g of the pre-liquid into a stirring kettle equipped with a high-speed stirrer, and then adding 5 g of hydroxyproline and mixing them evenly, heating the mixture to 35° C., and then slowly adding 20 g of the base liquid at a speed of 1.5 mL / min under stirring conditions, and homogenizing at a speed of 1500 rpm to obtain a liquid to be dried;

[0094] 5) The liquid to be dried is heated to volatilize and remove the solvent and the organic amine, and deionized water is continuously added during the removal process until the residual amount of the alcohol solvent and the organic amine is detected to be less than 300 ppm, and then freeze-dried to obtain the product.

[0095] The alcohol solvent is propanol and the modified cellulose is sodium carboxymethyl cellulose.

[0096] Performance testing

[0097] 1. Dissolution test

[0098] 1.1 Prepare rutin standard solution, accurately prepare rutin standard solution with a concentration of 1 mg / mL, accurately draw 0.2 / 0.3 / 0.4 / 0.5 / 0.6 / 0.7 mL of rutin standard solution, fill the volume to 1 mL, add 0.3 mL of 5% sodium nitrate solution, shake well and let stand for 10 minutes, then add 0.3 mL of 10% aluminum nitrate solution, shake well and let stand for 10 minutes, then add 4 mL of 1 mol / L sodium hydroxide solution, add 0.4 mL of high-purity water, shake well and let stand for 10 minutes, use blank reagent as control, and measure the absorbance of the solution at a wavelength of 510 nm. Draw a standard curve with rutin concentration as the horizontal axis and absorbance as the vertical axis.

[0099] Take 1g of seabuckthorn instant tea of ​​Examples 1-3 and Comparative Examples 1-3, and test it according to the dissolution determination method (rotating paddle method), test the dissolution value according to 5s, 10s, 20s, 45s, and 60s as the detection time points, the sampling volume is 5mL, the sampling point is the midpoint from the top of the paddle to the liquid surface, filter with a microporous filter membrane immediately after sampling, and the filtration should be completed within 30 seconds. After filtration, test the absorbance according to the method of 1.1, and calculate the amount of flavonoids in the samples sampled at different times from the standard curve, and then take 1g of seabuckthorn instant tea of ​​Examples 1-3 and Comparative Examples 1-3, and also test it according to the dissolution determination method, with 10min as the detection time point as the comparison group, and take the flavonoid amount in the comparison group as the benchmark, dissolution = flavonoid concentration in sample × sample volume / flavonoid amount in comparison group × 100%, specific test data see Figure 1 .

[0100] Analyze Examples 1-3 and Comparative Examples 1-3 and combine Figure 1It can be seen that the instant tea of ​​the present invention has a very good dissolution effect, has high dispersibility and solubility of flavonoids and insoluble substances, and can form a stable and transparent tea soup.

[0101] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A method for preparing seabuckthorn instant tea, characterized in that: The steps include: 1) Wash the seabuckthorn leaves, select and remove impurities, crush them into powder, sieve and dry them to obtain seabuckthorn leaf powder; 2) mixing an alcohol solvent and seabuckthorn leaf powder evenly, letting it stand and then heating for extraction, and obtaining an extract after solid-liquid separation; 3) taking the leaching solution and deionized water and mixing them evenly to obtain a pre-fluid, then adding calcium chloride and an organic amine into the pre-fluid, stirring to completely dissolve the calcium chloride, and then introducing carbon dioxide to obtain an intermediate solution; 4) Add modified cellulose and water into a container and mix well to obtain a base liquid; then add hydroxyproline to the intermediate liquid, raise the temperature to 35-50° C., then slowly add the base liquid, and homogenize to obtain a liquid to be dried; 5) The liquid to be dried is heated to evaporate and remove the solvent and organic amine, and then freeze-dried to obtain the product.

2. The method for preparing seabuckthorn instant tea according to claim 1, characterized in that: In the step 3), one or more of the following technical features are also included: 21) The mass proportion of the extract in the pre-liquid is 60-75%; 22) The organic amine is one or more of dimethylamine, trimethylamine, diethylamine and triethylamine; 23) The molar ratio of calcium chloride to organic amine is 1:20-30; 24) The molar ratio of carbon dioxide to calcium chloride is (2.5-3):1; 25) The mass ratio of pre-liquid to calcium chloride is 1:(0.005-0.01).

3. The method for preparing seabuckthorn instant tea according to claim 1, characterized in that: In the step 2), the alcohol solvent is a low dielectric constant alcohol solvent.

4. The method for preparing seabuckthorn instant tea according to claim 3, characterized in that: The low dielectric constant alcohol solvent is ethanol or propanol.

5. The method for preparing seabuckthorn instant tea according to claim 1, characterized in that: In the step 4), one or more of the following technical features are also included: 41) The mass ratio of the base liquid, hydroxyproline and the intermediate liquid is (0.15-0.2):(0.03-0.05):1; 42) Slowly adding the intermediate solution means first adding 30-50% of the total volume of the intermediate solution at a rate of 0.5-1 mL / min, and then adding the remaining intermediate solution at a rate of 2-3.5 mL / min; 43) After slowly adding the intermediate solution, immediately place it in a cold trap to cool to 2-5°C.

6. The method for preparing seabuckthorn instant tea according to claim 1, characterized in that: In the step 4), the modified cellulose is one or more of sodium carboxymethyl cellulose, sodium carboxyethyl cellulose, and hydroxypropyl methyl cellulose.

7. The method for preparing seabuckthorn instant tea according to claim 1, characterized in that: In the step 4), the mass proportion of the modified cellulose in the base liquid is 0.3-0.5%.

8. The method for preparing seabuckthorn instant tea according to claim 1, characterized in that: In the step 4), a sorbitol preservative is also added during the homogenization process.

9. The method for preparing seabuckthorn instant tea according to claim 1, characterized in that: In the step 4), a sweetener is also added during the homogenization process, and the sweetener is one or more of sucrose, erythritol, glucose, acesulfame potassium, and aspartame.

10. The method for preparing seabuckthorn instant tea according to claim 1, characterized in that: In the step 4), the homogenization is carried out at a speed of 1500-2000 rpm.

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