Anti-bacterial gel containing active umbilical cord extract exosome and preparation method of anti-bacterial gel

Abstract

The invention relates to the field of anti-bacterial and bacteriostatic gel, in particular to anti-bacterial and bacteriostatic gel containing active umbilical cord extract exosome and a preparation method of the anti-bacterial and bacteriostatic gel. The active umbilical cord extract exosome and the three traditional Chinese medicine extracts are extracted and combined to prepare the novel exosome active anti-bacterial gel, and the novel exosome active anti-bacterial gel can be smeared on the surface of skin for use and can also be pushed into the vagina of a woman by adopting an injection type propeller. After the anti-bacterial gel entering the female vagina is in contact with the mucosa of the human body, the anti-bacterial gel begins to dissolve along with temperature rise, the umbilical cord extract exosome and the bacteriostatic agent in the anti-bacterial gel start to play an active role, the traditional Chinese medicine extract plays a repairing role, the umbilical cord extract exosome also plays a regenerating role, and sodium hyaluronate starts to establish a barrier; the contact between harmful bacteria and mucous membranes is blocked. A complete ecological circle is formed, and the purposes of resisting bacteria, inhibiting bacteria, repairing, preserving moisture and moistening are achieved.

Description

Technical Field

[0001] The present invention relates to the field of antibacterial gel, and in particular to an antibacterial gel containing active umbilical cord extract exosomes and a preparation method thereof. Background Art

[0002] Antibacterial gels can be divided into many types according to their uses and ingredients. Common antibacterial gels denature and coagulate the proteins of microorganisms through chemical reactions, thereby killing or inhibiting the growth of microorganisms, but they are highly irritating to the skin and are not suitable for female vaginal care. Summary of the invention

[0003] In view of the problems existing in the background technology, an antibacterial gel containing active umbilical cord extract exosomes and a preparation method thereof are proposed.

[0004] The present invention provides an antibacterial gel containing active umbilical cord extract exosomes, which comprises, according to the formula ratio: 0.3-0.5% of p-hydroxyacetophenone, 0.5-2% of umbilical cord extract exosomes, 0.2-0.9% of sodium hyaluronate, 0.5-10% of dandelion extract, 0.5-10% of motherwort extract, 0.5-5% of sophora flavescens extract, 0.8-1.5% of lactic acid, 75-95% of water, 0.2-0.5% of carbomer and 0.5-2% of amino acid mixture.

[0005] Preferably, the amino acid mixture comprises 0.345-1.38% water, 0.15-0.6% butanediol, 0.001255-0.00502% glycine, 0.000695-0.00278% serine, 0.000645-0.00258% glutamic acid, 0.000405-0.00162% aspartic acid, 0.000355-0.00142% leucine, 0.000225-0.0009% alanine, 0.00021-0 0.00084% lysine, 0.00019-0.00076% arginine, 0.000175-0.0007% tyrosine, 0.000165-0.00066% phenylalanine, 0.000155-0.00062% threonine, 0.000155-0.00062% proline, 0.000155-0.00062% valine, 0.00014-0.00056% isoleucine and 0.000075-0.0003% histidine.

[0006] The present invention further proposes a method for preparing the antibacterial gel containing active umbilical cord extract exosomes, the steps of which are as follows:

[0007] S1. Prepare umbilical cord extract exosome raw materials for later use;

[0008] S2, dissolving water, p-hydroxyacetophenone and sodium hyaluronate, mixing and adding to the umbilical cord extract exosomes for later use;

[0009] S3, adding carbomer to water and soaking for 4-6 hours, mixing evenly to form a base gel for later use;

[0010] S4, adding the three extract compositions of dandelion extract, motherwort extract and sophora flavescens extract into the base gel, stirring evenly, and setting aside;

[0011] S5, add the mixture in S2 to the base gel, stir evenly, and set aside;

[0012] S6, adding lactic acid to the base gel and stirring evenly to prepare an antibacterial gel semi-finished product for standby use;

[0013] S7, adding the amino acid mixture to the semi-finished antibacterial gel, stirring evenly at low temperature to make a finished antibacterial gel, and letting it stand for inspection;

[0014] S8, inspection, filling gel tubes, wrapping in aluminum foil bags, packing in outer packaging boxes (3.0g / tube), packing, and storage.

[0015] Preferably, the lactic acid is the antibacterial agent L-lactic acid.

[0016] Preferably, an injection-type propeller is used to package the antibacterial gel.

[0017] Compared with the prior art, the present invention has the following beneficial technical effects: the present invention combines active umbilical cord extract exosomes and three kinds of Chinese medicine extracts to prepare a new exosome active antibacterial gel, which can be applied to the skin surface for use, or the antibacterial gel can be pushed into the female vagina by an injection propeller. After the antibacterial gel enters the female vagina and contacts the human mucosa, it begins to melt as the temperature rises, and the umbilical cord extract exosomes and antibacterial agents in the antibacterial gel begin to play an active role, the Chinese medicine extract plays a repairing role, and the umbilical cord extract exosomes play a regenerative role, and sodium hyaluronate begins to establish a barrier to block the contact between harmful bacteria and mucosa. A complete ecosystem is formed to achieve the purpose of antibacterial, antibacterial, repair, moisturizing and nourishing. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The present invention is a flowchart of the method for preparing the antibacterial gel containing active umbilical cord extract exosomes;

[0019] Figure 2 It is a schematic diagram of the structure of the injection thruster in the present invention.

[0020] Figure numerals: 1. plug; 2. antibacterial gel body; 3. gel tube; 4. push rod. DETAILED DESCRIPTION

[0021] Embodiment 1

[0022] The present invention provides an antibacterial gel containing active umbilical cord extract exosomes, which comprises, according to the formula ratio: 0.3-0.5% of p-hydroxyacetophenone, 0.5-2% of umbilical cord extract exosomes, 0.2-0.9% of sodium hyaluronate, 0.5-10% of dandelion extract, 0.5-10% of motherwort extract, 0.5-5% of sophora flavescens extract, 0.8-1.5% of lactic acid, 75-95% of water, 0.2-0.5% of carbomer and 0.5-2% of amino acid mixture.

[0023] It should be further explained that the amino acid mixture is 0.345-1.38% water, 0.15-0.6% butanediol, 0.001255-0.00502% glycine, 0.000695-0.00278% serine, 0.000645-0.00258% glutamic acid, 0.000405-0.00162% aspartic acid, 0.000355-0.00142% leucine, 0.000225-0.0009% alanine, 0.00021-0 0.00084% lysine, 0.00019-0.00076% arginine, 0.000175-0.0007% tyrosine, 0.000165-0.00066% phenylalanine, 0.000155-0.00062% threonine, 0.000155-0.00062% proline, 0.000155-0.00062% valine, 0.00014-0.00056% isoleucine and 0.000075-0.0003% histidine.

[0024] Embodiment 2

[0025] like Figure 1 As shown, the present invention further proposes a method for preparing the above-mentioned antibacterial gel containing active umbilical cord extract exosomes, the steps are as follows:

[0026] S1. Prepare umbilical cord extract exosome raw materials for later use;

[0027] S2, dissolving water, p-hydroxyacetophenone and sodium hyaluronate, mixing and adding to the umbilical cord extract exosomes for later use;

[0028] S3, adding carbomer to water and soaking for 4-6 hours, mixing evenly to form a base gel for later use;

[0029] S4, adding the three extract compositions of dandelion extract, motherwort extract and sophora flavescens extract into the base gel, stirring evenly, and setting aside;

[0030] S5, add the mixture in S2 to the base gel, stir evenly, and set aside;

[0031] S6, adding lactic acid to the base gel and stirring evenly to prepare an antibacterial gel semi-finished product for standby use;

[0032] S7, adding the amino acid mixture to the semi-finished antibacterial gel, stirring evenly at low temperature to make a finished antibacterial gel, and letting it stand for inspection;

[0033] S8, inspection, filling gel tubes, wrapping in aluminum foil bags, packing in outer packaging boxes (3.0g / tube), packing, and storage.

[0034] It should be further explained that lactic acid is an antibacterial agent, L-lactic acid. L-lactic acid is present in yogurt and tomato juice, is non-toxic, and has good water solubility. After use, it can protect the skin, play a role in moisturizing and hydrating, and help lock moisture on the skin surface to prevent symptoms such as dryness and peeling caused by skin dehydration. Lactic acid and glycolic acid have similar effects, have a strong transdermal effect, and present normal carbonate metabolism in the skin. After L-lactic acid is applied to the skin surface, it can promote the generation of keratinocytes, increase the moisture in the dermis, thicken the dermis, dilate capillaries, and help improve skin quality. External use of low concentrations can improve skin metabolism, accelerate the division of keratinocytes, and thus cause the accumulated keratinocytes to fall off. It can also increase the water content of keratinocytes and dermis, increase the number of mucopolysaccharides, collagen fibers, and elastic fibers in the dermis, increase the thickness of the dermis, and dilate superficial capillaries to improve skin texture. It is used for dry skin, senile skin pruritus, etc. After the body uses L-lactic acid properly, it can promote skin metabolism, speed up the renewal of skin tissues, help improve sebum accumulation in pores, and reduce enlarged pores. It can inhibit the activity of tyrosinase and has an outstanding whitening effect. Since lactic acid is also a component of the skin's natural moisturizing factor, it exceeds the effect of traditional moisturizers and hygroscopic agents and can significantly increase the water content of the stratum corneum. It is suitable for skin care, hair care and various body care products.

[0035] It should be further explained that the antibacterial gel is packaged with an injection-type propeller. The injection propeller has good stability, is sealed, safe and hygienic, is one at a time, is individually packaged, prevents cross infection, is easy to carry, and is very popular among women.

[0036] It should be further explained that the specific structure of the injection thruster is as follows: Figure 2 As shown, it is composed of a plug 1, an antibacterial gel body 2, a gel tube 3 and a push rod 4.

[0037] It should be further explained that the preparation method of exosome raw materials for umbilical cord extract is as follows: Select the umbilical cord of healthy animals (domestic pigs, buffaloes, goats). Ensure that the source is healthy, qualified for quarantine, non-contaminated, and non-coagulated. The umbilical cord is collected within 30 minutes after the animal gives birth to maximize the retention of active ingredients. It can be stored in a low-temperature freezer at -80°C for up to 5 years, which greatly improves the utilization rate of the umbilical cord. Thawing is carried out by ice water bath at 4°C to minimize the loss of active ingredients. During the thawing process, shaking and freezing should be avoided to prevent the umbilical cord tissue from breaking. Soak and rinse the tissue with sterile saline to remove blood and other impurities on the tissue. All instruments during the cleaning process should be sterile and strictly disinfected. Use a biological tissue grinder for crushing to more effectively extract the active ingredients. The crushing process needs to be carried out in an environment that complies with safety regulations to avoid cross contamination. The crushed umbilical cord is cryogenically frozen again to prevent the decomposition of the active ingredients. Attention should be paid to temperature control during the freezing process to prevent the structure of the umbilical cord tissue from breaking. Excessive evaporation of water should be avoided during the freezing process to prevent the extraction efficiency from decreasing. Add bioactive enzymes to open the cell walls and extract the active substances in the cells. Determine the time for enzymatic hydrolysis. If it is too short, the extraction will be incomplete, and if it is too long, the active substances in the cells may be damaged. The temperature and pH value need to be controlled during the enzymatic hydrolysis to ensure the activity of the enzyme. Sterilize using a steam sterilizer. Perform a sterility test after sterilization to confirm the sterilization effect. Use a 0.22 micron filter to ensure that the filtered cell fragments and microorganisms are intercepted. Avoid the formation of bubbles during the filtration process to prevent the filter from clogging and rupture. After the filtration is completed, test the filtrate to confirm the presence of active substances and the absence of microorganisms. Mix the filtered extract with other ingredients (tetrahydromethylpyrimidine carboxylic acid, butanediol, 1,2-hexanediol, and p-hydroxyacetophenone) to improve the stability and quality of the product. Use a magnetic stirrer to fully mix the umbilical cord extract. Ensure that the mixing process is carried out in a sterile environment and mix the required ingredients in the correct proportion according to the formulation requirements and standard operating procedures. Perform quality inspection on the mixed extract to ensure that the content of active substances in the product meets the standards.

[0038] Umbilical cord extract exosomes are tiny vesicles (30-150nm wide) secreted by stem cells. They are similar to "communication soldiers" between exosomes and can transfer DNA, RNA or proteins (including cytokines) between cells. Thereby affecting the function of receptor cells. It can promote the regeneration of skin-related fibroblasts and keratinocytes, while secreting collagen, elastic fibers and matrix components to achieve the function of repairing the skin. It can promote the angiogenesis required for the growth and nutrition supply of skin cells, inhibit excessive inflammatory reactions, and inhibit scar formation.

[0039] It should be further explained that the preparation methods of dandelion extract, motherwort extract and sophora flavescens extract are as follows: the dandelion extract, motherwort extract and sophora flavescens extract are screened and carefully inspected, cut into 2 cm small sections, and then ground into powder; 90-degree medical wine is added to the powder for soaking, and then ethanol is removed, filtered and decolorized to obtain dandelion extract, motherwort extract and sophora flavescens extract.

[0040] The present invention combines active umbilical cord extract exosomes and three kinds of Chinese medicine extracts to prepare a new exosome active antibacterial gel, which can be applied to the skin surface for use, or an injection-type propeller can be used to push the antibacterial gel into the female vagina. After the antibacterial gel enters the female vagina and contacts the human mucosa, it begins to melt as the temperature rises, and the umbilical cord extract exosomes and antibacterial agents in the antibacterial gel begin to play an active role, the Chinese medicine extract plays a repairing role, and the umbilical cord extract exosomes play a regenerative role, and sodium hyaluronate begins to establish a barrier to block the contact between harmful bacteria and mucosa. A complete ecosystem is formed to achieve the purposes of antibacterial, antibacterial, repair, moisturizing and nourishing.

[0041] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited thereto, and various changes can be made within the knowledge scope of technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. An antibacterial gel containing active umbilical cord extract exosomes, characterized in that: The formula includes: 0.3-0.5% parahydroxyacetophenone, 0.5-2% umbilical cord extract exosomes, 0.2-0.9% sodium hyaluronate, 0.5-10% dandelion extract, 0.5-10% motherwort extract, 0.5-5% sophora flavescens extract, 0.8-1.5% lactic acid, 75-95% water, 0.2-0.5% carbomer and 0.5-2% amino acid mixture.

2. The antibacterial gel containing active umbilical cord extract exosomes according to claim 1, characterized in that: The amino acid mixture is 0.345-1.38% water, 0.15-0.6% butanediol, 0.001255-0.00502% glycine, 0.000695-0.00278% serine, 0.000645-0.00258% glutamic acid, 0.000405-0.00162% aspartic acid, 0.000355-0.00142% leucine, 0.000225-0.0009% alanine, 0.00021-0.0008 4% lysine, 0.00019-0.00076% arginine, 0.000175-0.0007% tyrosine, 0.000165-0.00066% phenylalanine, 0.000155-0.00062% threonine, 0.000155-0.00062% proline, 0.000155-0.00062% valine, 0.00014-0.00056% isoleucine and 0.000075-0.0003% histidine.

3. The method for preparing the antibacterial gel containing active umbilical cord extract exosomes according to claim 2, characterized in that: Here are the steps: S1. Prepare umbilical cord extract exosome raw materials for later use; S2, dissolving water, p-hydroxyacetophenone and sodium hyaluronate, mixing and adding to the umbilical cord extract exosomes for later use; S3, adding carbomer to water and soaking for 4-6 hours, mixing evenly to form a base gel for later use; S4, adding the three extract compositions of dandelion extract, motherwort extract and sophora flavescens extract into the base gel, stirring evenly, and setting aside; S5, add the mixture in S2 to the base gel, stir evenly, and set aside; S6, adding lactic acid to the base gel and stirring evenly to prepare an antibacterial gel semi-finished product for standby use; S7, adding the amino acid mixture to the semi-finished antibacterial gel, stirring evenly at low temperature to make a finished antibacterial gel, and letting it stand for inspection; S8, inspection, filling gel tubes, wrapping in aluminum foil bags, packing in outer packaging boxes (3.0g / tube), packing, and storage.

4. The method for preparing the antibacterial gel containing active umbilical cord extract exosomes according to claim 3, characterized in that: Lactic acid is an antibacterial agent, L-lactic acid.

5. The method for preparing the antibacterial gel containing active umbilical cord extract exosomes according to claim 3, characterized in that: The antibacterial gel is packaged using an injection-type propeller.

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