Anti-bacteriostatic agent containing cell extract exosome and preparation method of anti-bacteriostatic agent

Abstract

The invention relates to the field of female private care agents, in particular to an anti-bacteriostatic agent containing a cell extract exosome and a preparation method of the anti-bacteriostatic agent. The composition comprises a carrier component and a content component, wherein the content component is prepared from the following components in percentage by weight: an active composition comprising 1-3% of purified water, 0.16-5% of leukocyte extract exosome, 0.2-0.4% of sodium propionate and 2-3% of glycerol; the solubilizer is prepared from 1 to 2 percent of polysorbate-80; the traditional Chinese medicine anti-bacterial composition is prepared from 0.3 to 5 percent of radix sophorae flavescentis, 0.3 to 5 percent of honeysuckle flower, 0.5 to 5 percent of herba leonuri, 0.5 to 5 percent of radix angelicae sinensis, 2 to 20 percent of radix arnebiae seu lithospermi, 5 to 15 percent of fructus cnidii, 3 to 5 percent of aloe, 5 to 15 percent of viburnum venetum and 50 to 60 percent of fructus hippophae seed oil; the auxiliary agent comprises 0.16-9% of sweet orange essential oil and 30-50% of sea buckthron oil; the bacteriostatic agent is prepared from 0.1 to 2 percent of chlorhexidine acetate. According to the invention, the active traditional Chinese medicine extract plays a repairing role, the leukocyte exosome contains anti-inflammatory interleukin, and the traditional Chinese medicine composition and the plant extract establish a barrier to block contact between harmful bacteria and mucous membranes. And a complete antibacterial, bacteriostatic, repairing, moisturizing and maintaining ecological circle is formed.

Description

Technical Field

[0001] The present invention relates to the field of female private care products, and in particular to an antibacterial agent containing cell extract exosomes and a preparation method thereof. Background Art

[0002] Antibacterial gels can be divided into many types according to their uses and ingredients. Common antibacterial gels denature and coagulate the proteins of microorganisms through chemical reactions, thereby killing or inhibiting the growth of microorganisms. However, they are not suitable for female vaginal care and cannot meet women's needs for vaginal antibacterial repair and firming effects. Summary of the invention

[0003] In view of the problems existing in the background technology, an antibacterial agent containing cell extract exosomes and a preparation method thereof are proposed.

[0004] The present invention provides an antibacterial agent containing cell extract exosomes, comprising a carrier component and a content component: the content component comprises an active composition, a solubilizing agent, a traditional Chinese medicine antibacterial composition, an adjuvant and an antibacterial agent, and the specific proportions are as follows: the active composition comprises 1-3% purified water, 0.16-5% leukocyte extract exosomes, 0.2-0.4% sodium propionate and 2-3% glycerol; the solubilizing agent comprises 1-2% polysorbate-80; the traditional Chinese medicine antibacterial composition comprises 0. 3-5% of Sophora flavescens, 0.3-5% of Honeysuckle, 0.5-5% of Leonurus japonicus, 0.5-5% of Angelica sinensis, 2-20% of Lithospermum officinale, 5-15% of Cnidium monnieri, 3-5% of Aloe vera, 5-15% of Mangakali, 50-60% of Seabuckthorn seed oil; adjuvants include 0.16-9% of sweet orange essential oil and 30-50% of Seabuckthorn oil; antibacterial agents include 0.1-2% of chlorhexidine acetate; the carrier component ratio includes 30% of gelatin and 70% of water.

[0005] Preferably, it can be prepared into antibacterial liquid, antibacterial capsule or antibacterial gel.

[0006] The present invention further proposes a method for preparing the antibacterial agent containing cell extract exosomes, the steps of which are as follows:

[0007] S1. Take 1-2% of the solubilizer polysorbate 80 in the formula and add it to 1-3% of purified water, stir evenly and set aside;

[0008] S2, take 0.16%-5% of exosomes from leukocyte extract and add it to 2-3% of glycerol, stir evenly, then add 0.2-0.3% of sodium propionate and stir evenly, then add it to S1 to dissolve, mix and stir evenly and set aside;

[0009] S3, select Chinese medicinal materials of 0.5-5% of Angelica sinensis, 2-20% of Lithospermum officinale, 5-15% of Cnidium monnieri, 3-5% of Aloe vera, 5-15% of Mangakali, 0.3-5% of Sophora flavescens, 0.3-5% of Honeysuckle and 0.5-5% of Leonurus japonicus, carefully inspect, cut, soak in 50-60% of sea buckthorn seed oil, start to boil after 30 minutes, boil for 1 hour, filter after natural cooling, take the filtrate oil, and set aside;

[0010] S4, taking 25-33% of sea buckthorn seed oil, adding 16-9% of sweet orange essential oil and stirring evenly, then adding 0.1-2% of antibacterial agent chlorhexidine acetate, stirring evenly and setting aside.

[0011] S5, soak gelatin and purified water in a ratio of 3:7 for 4-6 hours, mix well, and make gelatin sheets for later use, ready to be pressed into capsules by machine;

[0012] S6, adding the products of S2 and S4 to the product of S3, stirring them evenly to prepare a semi-finished active antibacterial oil for standby use;

[0013] S7, add the semi-finished active antibacterial oil of S6 product into the liquid medicine tank of the capsule wrapping machine, place the prepared gelatin sheets on the film racks on both sides of the capsule wrapping machine, start to make soft capsule capsules, wrap the gelatin sheets with antibacterial liquid, make capsules of the same size as required specifications, and finalize;

[0014] S8, inspection, packaging (0.6g / pill), boxing, and storage.

[0015] Compared with the prior art, the present invention has the following beneficial technical effects:

[0016] The antibacterial agent of the present invention can be made into an antibacterial liquid and applied to the skin for use. It can also be made into a capsule, which is placed in the private vagina of a woman with a medical hygiene fingertip, or it can be made into a female private antibacterial gel and pushed into the vagina with a private protection thruster. Taking the antibacterial capsule as an example, its outer layer is wrapped with gelatin, which is similar to the lipid encapsulation technology. The gelatin replaces the lipid layer, and the gelatin layer has only 1 mm of outer skin. The finished product contacts the human mucosa. As the temperature rises, the capsule in contact with the private vagina begins to dissolve, and the exosome activity and antibacterial agent in the antibacterial capsule begin to play a role. The active Chinese medicine extract plays a repairing role. The leukocyte exosome itself contains a large amount of interleukin, which has an anti-inflammatory effect and assists the tissue to play a regenerative role. The Chinese medicine composition and plant extract begin to establish a barrier to block the contact between harmful bacteria and mucosa. Form a complete antibacterial, antibacterial, repair, moisturizing and maintenance ecosystem. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a finished product display diagram of the present invention;

[0018] Figure 2 A flow chart of the method for preparing an antibacterial agent containing exosomes from cell extracts of the present invention;

[0019] Figure 3 It is a schematic diagram of the processing equipment in the present invention;

[0020] Figure 4 This is a picture of the mucosa after using antibacterial capsules.

[0021] The attached drawings are marked with: 1. antibacterial capsules; 2. rubber skin; 3. antibacterial liquid. DETAILED DESCRIPTION

[0022] Embodiment 1

[0023] like Figure 1 As shown, the present invention proposes an antibacterial agent containing cell extract exosomes, including a carrier component and a content component: the content component includes an active composition, a solubilizing agent, a traditional Chinese medicine antibacterial composition, an adjuvant and an antibacterial agent, and the specific composition is as follows: the active composition includes 1-3% purified water, 0.16-5% leukocyte extract exosomes, 0.2-0.4% sodium propionate and 2-3% glycerol; the solubilizing agent includes 1-2% polysorbate-80; the traditional Chinese medicine antibacterial composition includes 0.3-5% of Sophora flavescens, 0.3-5% of Honeysuckle, 0.5-5% of Leonurus japonicus, 0.5-5% of Angelica sinensis, 2-20% of Lithospermum officinale, 5-15% of Cnidium monnieri, 3-5% of Aloe vera, 5-15% of Mangakali, 50-60% of Seabuckthorn seed oil; adjuvants include 0.16-9% of sweet orange essential oil and 30-50% of Seabuckthorn oil; antibacterial agents include 0.1-2% of chlorhexidine acetate; the carrier component ratio includes 30% of gelatin and 70% of water.

[0024] Embodiment 2

[0025] like Figure 2 As shown, the present invention further proposes a method for preparing the above-mentioned antibacterial agent containing cell extract exosomes, the steps are as follows:

[0026] S1. Take 1-2% of the solubilizer polysorbate 80 in the formula and add it to 1-3% of purified water, stir evenly and set aside;

[0027] S2, take 0.16%-5% of exosomes from leukocyte extract and add it to 2-3% of glycerol, stir evenly, then add 0.2-0.3% of sodium propionate and stir evenly, then add it to S1 to dissolve, mix and stir evenly and set aside;

[0028] S3, select Chinese medicinal materials of 0.5-5% of Angelica sinensis, 2-20% of Lithospermum officinale, 5-15% of Cnidium monnieri, 3-5% of Aloe vera, 5-15% of Mangakali, 0.3-5% of Sophora flavescens, 0.3-5% of Honeysuckle and 0.5-5% of Leonurus japonicus, carefully inspect, cut, soak in 50-60% of sea buckthorn seed oil, start to boil after 30 minutes, boil for 1 hour, filter after natural cooling, take the filtrate oil, and set aside;

[0029] S4, taking 25-33% of sea buckthorn seed oil, adding 16-9% of sweet orange essential oil and stirring evenly, then adding 0.1-2% of antibacterial agent chlorhexidine acetate, stirring evenly and setting aside.

[0030] S5, soak gelatin and purified water in a ratio of 3:7 for 4-6 hours, mix well, and make gelatin sheets for later use, ready to be pressed into capsules by machine;

[0031] S6, adding the products of S2 and S4 to the product of S3, stirring them evenly to prepare a semi-finished active antibacterial oil for standby use;

[0032] S7, add the semi-finished active antibacterial oil of S6 product into the liquid medicine tank of the capsule wrapping machine, place the prepared gelatin sheets on the film racks on both sides of the capsule wrapping machine, start to make soft capsule capsules, wrap the gelatin sheets with antibacterial liquid, make capsules of the same size as required specifications, and finalize;

[0033] S8, inspection, packaging (0.6g / pill), boxing, and storage.

[0034] It should be further explained that S7 adopts Figure 3 In the equipment shown, the rubber sheets 2 prepared by the rubber wheel boxes on both sides of the main machine relatively enter the gap between the rolling dies, and the antibacterial liquid 3 is injected into the wedge-shaped spray body through the catheter by the feed pump. The antibacterial liquid 3 and the rubber sheets 2 are pressed into the grooves of the two rolling dies with the help of the pressure of the feed pump. Due to the continuous rotation of the rolling dies, the two rubber sheets 2 are formed into two semi-defined shapes, and the antibacterial liquid 3 is encapsulated in the capsule film to form a complete antibacterial capsule 1. The remaining rubber sheets 2 are cut and separated into a mesh, which can be recycled and reused after inspection and sterilization.

[0035] It should be further explained that the preparation method of the Chinese medicine anti-bacterial composition is as follows: Sophora flavescens, honeysuckle, motherwort, angelica, lithospermum officinale, cnidium monnieri, aloe and philadelphica oleracea are screened and finely inspected, cut into small pieces, and then soaked in seabuckthorn oil for 30 minutes and boiled for 2 hours. After natural cooling, the extracted oil is obtained, and finally the residue is filtered and removed to obtain the Chinese medicine anti-bacterial composition.

[0036] It should be further explained that the preparation process of the above-mentioned Chinese herbal antibacterial composition requires stainless steel instruments, a medicine cutter, a sieve, a steam-heated extraction reaction axe, a 60-mesh filter and other auxiliary container equipment.

[0037] It should be further explained that the preparation process of leukocyte extract exosomes is as follows:

[0038] 1. Healthy blood:

[0039] (1) Select fresh anticoagulated blood from healthy livestock (pigs, buffaloes, goats).

[0040] (2) Ensure that the source is healthy, has passed quarantine, is free of contamination, and does not contain blood clots.

[0041] 2. Centrifugal separation:

[0042] (1) Send the blood into a centrifuge for centrifugation to separate mononuclear cells.

[0043] (2) The mononuclear cells obtained by centrifugation are cultured in a culture medium containing a cell culture additive.

[0044] (3) Attention should be paid to the speed control during centrifugation to prevent the cell structure from being broken.

[0045] 3. Cultivation:

[0046] (1) Place the culture flask in an incubator containing 5% CO2 and culture at 37°C.

[0047] (2) Replace the cell culture medium every 24 hours, discard the old cell culture medium, and add an equal amount of fresh culture medium and cell culture supplements.

[0048] 4. Centrifuge again:

[0049] (1) Mature mononuclear cells are extracted by centrifugation, and the leukocyte extract is collected and stored.

[0050] (2) Attention should be paid to the rotation speed control during centrifugation to prevent the cell structure from being broken.

[0051] 5. Filtering:

[0052] (1) Use a 0.22 micron filter to ensure that cell fragments and microorganisms are intercepted by the filter.

[0053] (2) The generation of bubbles needs to be avoided during the filtration process to prevent filter clogging and rupture.

[0054] (3) After filtration, the filtrate is tested to confirm the presence of active substances and the absence of microorganisms.

[0055] 6. Mixing:

[0056] (1) The filtered extract is mixed with other ingredients (tetrahydromethylpyrimidine carboxylic acid, butylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone) to improve the stability and quality of the product.

[0057] (2) Use a magnetic stirrer to mix the leukocyte extract thoroughly.

[0058] (3) Ensure that the mixing process is carried out in a sterile environment and that the required ingredients are mixed in the correct proportions according to the formulation requirements and standard operating procedures.

[0059] 7. Quality inspection, product filling and packaging:

[0060] (1) Perform quality control tests on leukocyte extracts for bacteria, fungi, endotoxins, and mycoplasma. (2) Fill and seal the product under a sterile environment to prevent contamination of the product during the packaging process. (3) After the product is packaged, clearly mark the product information on the outer packaging, including production date, expiration date, production batch, etc.

[0061] 8. Storage:

[0062] (1) Store packaged products at appropriate temperatures to maintain product stability and shelf life.

[0063] (2) Conduct regular inventory counts and quality monitoring to ensure the quality of inventory products.

[0064] (3) Storage conditions should meet the requirements of the product, including temperature, humidity, light, etc., and should be monitored and recorded during storage.

[0065] Leukocyte exosomes are tiny membrane vesicles secreted by cells with a lipid bilayer membrane structure, a diameter of about 30-150 nanometers, and contain various bioactive molecules such as proteins, lipids, and nucleic acids. Exosomes transmit information between cells, regulate the phenotype and function of cells, and participate in a variety of physiological and pathological processes. In the skin immune system, exosomes play an important role in balancing the protection of the body from external threats and avoiding excessive inflammatory responses. Exosomes secreted by leukocytes can affect the activity of other immune cells or non-immune cells, regulate skin immune responses, and maintain the health of the skin. In addition, exosomes secreted by leukocytes can also promote angiogenesis required for the nutrient supply of skin cell growth, inhibit excessive inflammatory responses, and inhibit scar formation, which is of great significance to skin health.

[0066] The interleukins, interferons and perforins contained in the leukocyte extract can activate the immune function of the vaginal epidermal mucosa, activate the CIK immune cells and NK natural killer cells in the epidermal mucosal tissue, effectively improve the immune cells' killing ability against HPV viruses, bacteria and fungi, and prevent and reduce the risk of HPV infection and microbial infection in the vagina.

[0067] Chlorhexidine acetate, a Western medicine name, is a biguanide compound. This product is highly sensitive to some Staphylococci, Streptococcus mutans, Streptococcus salivarius, Candida albicans, Escherichia coli, and anaerobic propionibacterium: moderately sensitive to Streptococcus haemophilus, and slightly sensitive to Proteus, Pseudomonas, Klebsiella, and Gram-negative cocci (such as Veillonella). Chlorhexidine acetate can be adsorbed on the permeability barrier of bacterial cells to allow cell contents to leak out. This product inhibits bacteria at low concentrations and kills bacteria at high concentrations. This product has anti-inflammatory effects and is suitable for disinfection of skin and mucous membranes, wound infection, vaginal infection, and treatment of uterine erosion. Cervical erosion, purulent vaginitis.

[0068] The antibacterial agent containing cell extract exosomes prepared by the present invention comprises a carrier component and a content component: the content component comprises an active composition, a solubilizing agent, a Chinese medicine antibacterial composition, an adjuvant and an antibacterial agent, and the specific proportion is as follows: the active composition comprises 1-3% purified water, 0.16-5% leukocyte extract exosomes, 0.2-0.4% sodium propionate and 2-3% glycerol; the solubilizing agent comprises 1-2% polysorbate-80; the Chinese medicine antibacterial composition comprises 0. 3-5% of Sophora flavescens, 0.3-5% of honeysuckle, 0.5-5% of Leonurus japonicus, 0.5-5% of Angelica sinensis, 2-20% of Lithospermum officinale, 5-15% of Cnidium monnieri, 3-5% of Aloe vera, 5-15% of Mangakali, 50-60% of Seabuckthorn seed oil; adjuvants include 0.16-9% of sweet orange essential oil and 30-50% of Seabuckthorn oil; antibacterial agent includes 0.1-2% of chlorhexidine acetate; carrier component ratio includes 30% of gelatin and 70% of water. The above antibacterial agent can be made into antibacterial liquid for application on the skin. It can also be made into capsules and put into the private part of female vagina with medical hygiene finger sleeves, or it can be made into female private antibacterial gel and pushed into the vagina with a private protection pusher.

[0069] Take the antibacterial capsule as an example. Its outer layer is wrapped with gelatin, which is similar to the lipid wrapping technology. Gelatin replaces the lipid layer. The outer skin of the gelatin layer is only 1 mm. The finished product contacts the human mucosa. As the temperature rises, the capsule in contact with the private vagina begins to dissolve. The exosome activity and antibacterial agent in the antibacterial capsule begin to play a role. The active Chinese medicine extract plays a repair role. The leukocyte exosome itself contains a large amount of interleukin. Interleukin has an anti-inflammatory effect and assists tissue regeneration. The combination of Chinese medicine and plant extracts begins to establish a barrier to block the contact between harmful bacteria and mucosa. Form a complete antibacterial, antibacterial, repair, moisturizing and maintenance ecosystem, the effect is as follows: Figure 4 shown.

[0070] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited thereto, and various changes can be made within the knowledge scope of technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. An antibacterial agent containing cell extract exosomes, characterized in that Including carrier components and content components: The content components include active composition, solubilizer, traditional Chinese medicine antibacterial composition, adjuvant and antibacterial agent, and the specific proportion is as follows: The active composition includes 1-3% purified water, 0.16-5% leukocyte extract exosomes, 0.2-0.4% sodium propionate and 2-3% glycerol; Solubilizers include 1-2% polysorbate-80; The Chinese medicine antibacterial composition comprises 0.3-5% of Sophora flavescens, 0.3-5% of Honeysuckle, 0.5-5% of Leonurus japonicus, 0.5-5% of Angelica sinensis, 2-20% of Lithospermum officinale, 5-15% of Cnidium monnieri, 3-5% of Aloe vera, 5-15% of Mangakali, and 50-60% of Seabuckthorn seed oil; Auxiliary agents include 0.16-9% sweet orange essential oil and 30-50% sea buckthorn oil; Antimicrobial agents include 0.1-2% chlorhexidine acetate; The carrier composition ratio includes 30% gelatin and 70% water.

2. The antibacterial agent containing cell extract exosomes according to claim 1, characterized in that Can be prepared as antibacterial solution.

3. The antibacterial agent containing cell extract exosomes according to claim 1, characterized in that The invention can be prepared into antibacterial capsules.

4. The antibacterial agent containing cell extract exosomes according to claim 1, characterized in that Can be prepared as antibacterial gel.

5. The method for preparing the antibacterial agent containing cell extract exosomes according to claim 1, characterized in that: Here are the steps: S1. Take 1-2% of the solubilizer polysorbate 80 in the formula and add it to 1-3% of purified water, stir evenly and set aside; S2, take 0.16%-5% of exosomes from leukocyte extract and add it to 2-3% of glycerol, stir evenly, then add 0.2-0.3% of sodium propionate and stir evenly, then add it to S1 to dissolve, mix and stir evenly and set aside; S3, select Chinese medicinal materials of 0.5-5% of Angelica sinensis, 2-20% of Lithospermum officinale, 5-15% of Cnidium monnieri, 3-5% of Aloe vera, 5-15% of Mangakali, 0.3-5% of Sophora flavescens, 0.3-5% of Honeysuckle and 0.5-5% of Leonurus japonicus, carefully inspect, cut, soak in 50-60% of sea buckthorn seed oil, start to boil after 30 minutes, boil for 1 hour, filter after natural cooling, take the filtrate oil, and set aside; S4, taking 25-33% of sea buckthorn seed oil, adding 16-9% of sweet orange essential oil and stirring evenly, then adding 0.1-2% of antibacterial agent chlorhexidine acetate, stirring evenly and setting aside. S5, soak gelatin and purified water in a ratio of 3:7 for 4-6 hours, mix well, and make gelatin sheets for later use, ready to be pressed into capsules by machine; S6, adding the products of S2 and S4 to the product of S3, stirring them evenly to prepare a semi-finished active antibacterial oil for standby use; S7, add the semi-finished active antibacterial oil of S6 product into the liquid medicine tank of the capsule wrapping machine, place the prepared gelatin sheets on the film racks on both sides of the capsule wrapping machine, start to make soft capsule capsules, wrap the gelatin sheets with antibacterial liquid, make capsules of the same size as required specifications, and finalize; S8, inspection, packaging (0.6g / pill), boxing, and storage.

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