Humic acid-containing water-soluble bacterial fertilizer capable of promoting growth and increasing yield and preparation method of humic acid-containing water-soluble bacterial fertilizer

Abstract

The invention discloses a humic acid-containing water-soluble bacterial fertilizer for promoting growth and increasing yield and a preparation method thereof. Comprising the following components in parts by weight: 15-50 parts of mineral source potassium humate, 10-30 parts of urea, 3-10 parts of potassium pyrophosphate, 2-8 parts of potassium nitrate, 2-8 parts of monopotassium phosphate, 2-8 parts of orthophosphoric acid, 0.5-5 parts of Tween 80, 0.5-3 parts of sodium carboxymethyl cellulose, 1-5 parts of propylene glycol, 10-30 parts of bacillus amyloliquefaciens subsp. Plantarum thallus coating liquid and 50-100 parts of water. Through the high temperature-stirring-emulsifying process, the concentration of the mineral source potassium humate is greatly improved and is 3-5 times of the concentration of humic acid in similar products on the market; meanwhile, multi-layer coating is carried out on thalli, so that the bacterial agent can stably survive in a hypertonic solution, and the blank of the humic acid-containing water-soluble bacterial fertilizer is filled up; the humic acid-containing water-soluble bacterial fertilizer prepared by the invention is small in dosage and convenient to use; compared with a control group, the soil enzyme activity can be remarkably improved, the development of capillary roots of crops is promoted, and the yield and quality of the crops are remarkably improved.

Description

Technical Field

[0001] The invention relates to a water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing production and a preparation method thereof, and in particular to a water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing production and containing high-concentration mineral source potassium humate and microbial agents and a preparation method thereof. Background Art

[0002] Humic acid water-soluble fertilizer (NY 1106-2010) is a new type of fertilizer proposed and classified by the Ministry of Agriculture and Rural Affairs. It is a liquid or solid water-soluble fertilizer produced and sold in the People's Republic of China, which is made of mineral humic acid in a proportion suitable for plant growth, with appropriate amounts of nitrogen, phosphorus, potassium macroelements or copper, iron, manganese, zinc, boron, and molybdenum trace elements added. Mineral humic acid is formed by a series of processes such as microbial decomposition, transformation, and geochemical action of animal and plant residues, extracted from mud, lignite, or wind-blown coal, and is a mixture of amorphous polymer compounds containing benzene nuclei, carboxyl groups, and phenolic hydroxyl groups. Humic acid raw materials are widely available, green and natural, with complex and diverse beneficial functional groups inside, and have direct or indirect biological activity. In agricultural planting, it can regulate the acid-base balance of the soil, especially promote the division and elongation of root cells, and can be compounded with macroelements and trace elements to enhance each other. Water-soluble fertilizers containing humic acid have a high effective absorption rate and a fast fertilizer effect. At present, there are clear fertilizer effect experimental reports on crops such as peppers, cabbage, tomatoes, and rice at home and abroad (see CNKI "Water-soluble fertilizers containing humic acid"), which will not be elaborated here.

[0003] The agricultural standard for water-soluble fertilizers containing humic acid (liquid products with a large number of elements) requires that the humic acid content be ≥30g / L and the content of a large number of elements be ≥200g / L. Upon inquiry, the humic acid index of the water-soluble fertilizer license containing humic acid registered by the enterprise is mostly 30g / L. The low concentration leads to fierce homogeneous competition and cannot meet the requirements of modern agricultural water-saving and weight-loss and aerial fertilization. The promoting effect of humic acid on plant growth has been confirmed by a large number of experiments. It can affect a variety of physiological activities of plants, including plant growth, absorption of nutrients, synthesis of proteins and nucleic acids, photosynthesis, respiration, and the activity of some enzymes. In addition, microorganisms are the current star of "weight loss and efficiency increase" in agricultural planting. They have a very high application value in agricultural sustainable development and have broad application prospects in improving soil, improving soil fertility, and increasing crop yield and quality. However, the research on the combination of humic acid fertilizers and microbial agents in academia and business circles is still blank.

[0004] The key issues that limit the combination of humic acid and microbial agents are 1. the proportion of mineral humic acid added; 2. the hyperosmotic resistance and protection process of the selected microbial strains; 3. reasonable processing technology to ensure that humic acid and microorganisms can form a stable system; 4. after the combination, they can enhance each other's synergy and promote the root system and yield of crops. Therefore, it is urgent to provide a ratio, preparation method and application of a water-soluble fertilizer containing high concentration of humic acid to improve the current situation. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing yield and a preparation method thereof, which has the characteristics of high-concentration mineral source potassium humate and stable bacterial agent.

[0006] In order to solve the above technical problems, the technical scheme of the present invention is: a water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing production, and its innovation point is: the water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing production comprises 15-50 parts by weight of potassium humate from mineral source, 10-30 parts by weight of urea, 3-10 parts by weight of potassium pyrophosphate, 2-8 parts by weight of potassium nitrate, 2-8 parts by weight of potassium dihydrogen phosphate, 2-8 parts by weight of orthophosphoric acid, 0.5-5 parts by weight of Tween 80, 0.5-3 parts by weight of sodium carboxymethyl cellulose, 1-5 parts by weight of propylene glycol, 10-30 parts by weight of Bacillus amyloliquefaciens plant subspecies bacterial body coating liquid, and 50-100 parts by weight of water;

[0007] Among them, the strain number of Bacillus amyloliquefaciens plantarum is CICC 21168.

[0008] Preferably, the water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing yield comprises 20-40 parts by weight of potassium humate from mineral source, 15-20 parts by weight of urea, 5-8 parts by weight of potassium pyrophosphate, 4-5 parts by weight of potassium nitrate, 3-6 parts by weight of potassium dihydrogen phosphate, 2-6 parts by weight of orthophosphoric acid, 1-3 parts by weight of Tween 80, 0.5-1 parts by weight of sodium carboxymethyl cellulose, 1-3 parts by weight of propylene glycol, 15-25 parts by weight of Bacillus amyloliquefaciens plant subspecies bacterial coating liquid, and 50-70 parts by weight of water;

[0009] Among them, the strain number of Bacillus amyloliquefaciens plantarum is CICC 21168.

[0010] Preferably, the water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing yield comprises 20 parts by weight of potassium humate from mineral source, 25 parts by weight of urea, 10 parts by weight of potassium pyrophosphate, 5 parts by weight of potassium nitrate, 5 parts by weight of potassium dihydrogen phosphate, 5 parts by weight of orthophosphoric acid, 2 parts by weight of Tween 80, 1 part by weight of sodium carboxymethyl cellulose, 2 parts by weight of propylene glycol, 20 parts by weight of Bacillus amyloliquefaciens plant subspecies bacterial coating liquid, and 60 parts by weight of water;

[0011] Among them, the strain number of Bacillus amyloliquefaciens plantarum is CICC 21168.

[0012] A method for preparing a water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production, which is used to prepare the water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production, comprises the following steps:

[0013] Step S1: preparing a nutrient broth medium in a fermentation tank, sterilizing it at high temperature and high pressure, and then inoculating it with Bacillus amyloliquefaciens plant subspecies, and fermenting it at 35° C. for 20 hours to obtain a fermentation liquid;

[0014] Step S2: The fermentation liquid in the fermenter in step S1 is centrifuged and dehydrated using a disc centrifuge to obtain bacterial sludge;

[0015] Step S3: adding Tween 80 and propylene glycol in the above weight ratio to the bacterial sludge, and mixing evenly to make the bacterial cells evenly dispersed to form a bacterial coating liquid;

[0016] Step S4: adding water in the above weight proportion to the preparation device, and sequentially adding potassium humate from mineral sources, urea, potassium pyrophosphate, potassium nitrate, and potassium dihydrogen phosphate in the above weight proportion, sealing, heating and stirring for 40 minutes at a heating temperature of 50°C-70°C, adding orthophosphoric acid in the above weight proportion after stirring, adjusting the pH to 7.5, and cooling to room temperature to obtain a coolant;

[0017] Step S5: adding the above-mentioned sodium carboxymethyl cellulose in the weight proportion to the cooling liquid, and emulsifying it using a high-speed shear emulsifier to obtain an emulsion;

[0018] Step S6: adding the bacterial coating solution obtained in step S3 to the emulsion obtained in step S5, stirring for 10-20 minutes, and obtaining a water-soluble bacterial fertilizer containing humic acid.

[0019] Preferably, the plant subspecies of Bacillus amyloliquefaciens in step S1 is derived from the School of Food Science and Engineering of China Agricultural University, and is currently deposited in the China Industrial Microbiological Culture Collection Center with a deposit number of CICC21168. The strain characteristics are: rod-shaped, G+, forming oval endogenous spores, aerobic, resistant to 1%-7% NaCl concentration of glucose, sucrose, maltose, galactose, trehalose acid production, lactose, fructose, mannitol, raffinose, xylose no acid production, no decomposition of starch, catalase and oxidase positive.

[0020] Preferably, in step S1, the sterilization temperature is 121° C. and the sterilization time is 15 minutes; and in step S2, the rotation speed of the disc centrifuge is 1000 r / min.

[0021] Preferably, the emulsification time of the high-speed shear emulsifier in step S5 is 20 minutes.

[0022] Preferably, in step S6, the stirring speed of the stirrer is 200 r / min.

[0023] Preferably, the preparation device in step S4 comprises a tank body, a cover plate arranged at an opening position on the tank body and assembled with the tank body, a feed port arranged on the cover plate and a vertically arranged drive motor, a stirring shaft arranged in the tank body and connected to the drive motor and vertically arranged, the tank body is provided with a control panel located on the side of the tank body and a discharge port located at the bottom of the tank body, and the tank body is provided with a pH meter and a thermometer extending into the tank body and feeding back signals to the control panels respectively;

[0024] A water inlet pipe connected to the tank body after the hot water pipe and the cold water pipe merge is arranged on the bottom of the tank body, and a first temperature sensing valve and a second temperature sensing valve are arranged on the hot water pipe and the cold water pipe respectively, and the first temperature sensing valve and the second temperature sensing valve respectively feedback the thermometer signal.

[0025] Preferably, the cover plate is provided with a bracket for mounting the drive motor; the stirring shaft is provided with a six-blade disc turbine stirrer and a three-impeller stirrer located in the middle and bottom of the stirring shaft; the pH meter and the thermometer are both provided with stainless steel electrode protective sleeves;

[0026] The discharge port is provided with a switch for closing or opening the discharge channel; the tank body is provided with a visual window with a volume scale on the side of the tank body;

[0027] At least three supporting legs are arranged on the tank body and at the bottom of the tank body, and one end of the supporting legs away from the tank body is fixedly connected to a bottom plate.

[0028] The advantages of the invention are as follows: the invention greatly improves the concentration of mineral source potassium humate through a high temperature-stirring-emulsification process, which is 3-5 times the humic acid concentration of similar products on the market; at the same time, by multi-layer coating of the bacterial body, the bacterial agent can stably survive in a hypertonic solution, filling the gap of water-soluble bacterial fertilizer containing humic acid; the water-soluble bacterial fertilizer containing humic acid prepared by the invention has a small dosage and is easy to use; compared with the control group, the soil enzyme activity can be significantly improved, the development of crop capillary roots can be promoted, and the crop yield and quality can be significantly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The invention discloses a water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing yield and a structural schematic diagram of a preparation device in a preparation method thereof.

[0030] Figure 2 The invention discloses a water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing yield and a schematic diagram of the internal structure of a preparation device in a preparation method thereof.

[0031] In the figure: 1-tank body, 2-cover plate, 3-feeding port, 4-driving motor, 5-stirring shaft, 6-control panel, 7-discharging port, 8-pH meter, 9-thermometer, 10-hot water pipe, 11-cold water pipe, 12-first temperature sensing valve, 13-second temperature sensing valve, 14-bracket, 15-six-arrow blade disc turbine agitator, 16-three-impeller agitator, 17-switch, 18-visual window, 19-support leg. DETAILED DESCRIPTION

[0032] Example 1

[0033] The humic acid-containing water-soluble bacterial fertilizer for promoting growth and increasing yield comprises 15 parts by weight of mineral source potassium humate, 18 parts by weight of urea, 8 parts by weight of potassium pyrophosphate, 5 parts by weight of potassium nitrate, 2 parts by weight of potassium dihydrogen phosphate, 2 parts by weight of orthophosphoric acid, 0.5 parts by weight of Tween 80, 0.5 parts by weight of sodium carboxymethyl cellulose, 1 part by weight of propylene glycol, 10 parts by weight of bacillus amyloliquefaciens plant subspecies bacterial body coating liquid, and 50 parts by weight of water; wherein the strain number of the bacillus amyloliquefaciens plant subspecies is CICC 21168.

[0034] The invention greatly improves the concentration of mineral source potassium humate through a high temperature-stirring-emulsification process, which is 3-5 times the humic acid concentration of similar products on the market; at the same time, by multi-layer coating of the bacterial body, the bacterial agent can stably survive in a hypertonic solution, filling the gap of water-soluble bacterial fertilizer containing humic acid; the water-soluble bacterial fertilizer containing humic acid prepared by the invention has a small dosage and is easy to use; compared with the control group, the soil enzyme activity can be significantly improved, the development of crop capillary roots can be promoted, and the crop yield and quality can be significantly improved.

[0035] The preparation method of the water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production of the present invention comprises the following steps:

[0036] Step S1: preparing a nutrient broth medium in a fermentation tank, sterilizing it at high temperature and high pressure, and then inoculating it with Bacillus amyloliquefaciens plant subspecies, and fermenting it at 35° C. for 20 hours to obtain a fermentation liquid;

[0037] Step S2: the fermentation liquid in the fermenter in step S1 is centrifuged and dehydrated using a disc centrifuge;

[0038] Step S3: adding Tween 80 and propylene glycol in the above weight ratio to the bacterial sludge, and mixing evenly to make the bacterial cells evenly dispersed to form a bacterial coating liquid;

[0039] Step S4: adding water in the above weight proportion to the preparation device, and sequentially adding potassium humate from mineral sources, urea, potassium pyrophosphate, potassium nitrate, and potassium dihydrogen phosphate in the above weight proportion, sealing, heating and stirring for 40 minutes at a heating temperature of 50°C-70°C, adding orthophosphoric acid in the above weight proportion after stirring, adjusting the pH to 7.5, and cooling to room temperature to obtain a coolant;

[0040] Step S5: adding the above-mentioned sodium carboxymethyl cellulose in the weight proportion to the cooling liquid, and emulsifying it using a high-speed shear emulsifier to obtain an emulsion;

[0041] Step S6: adding the bacterial coating solution obtained in step S3 to the emulsion obtained in step S5, stirring for 10-20 minutes, and obtaining a water-soluble bacterial fertilizer containing humic acid.

[0042] The plant subspecies of Bacillus amyloliquefaciens in step S1 is derived from the School of Food Science and Engineering of China Agricultural University and is currently deposited in the China Industrial Microbiological Culture Collection Center with a deposit number of CICC21168. The strain characteristics are: rod-shaped, G+, forming oval endogenous spores, aerobic, resistant to 1%-7% NaCl concentration of glucose, sucrose, maltose, galactose, trehalose acid production, lactose, fructose, mannitol, raffinose, xylose no acid production, no decomposition of starch, catalase and oxidase positive.

[0043] In the above step S1, the sterilization temperature is 121° C. and the sterilization time is 15 minutes; in step S2, the speed of the disc centrifuge is 1000 r / min; in step S5, the emulsification time of the high-speed shear emulsifier is 20 minutes; and in step S6, the stirring speed of the mixer is 200 r / min.

[0044] The preparation device in the above step S4 includes a tank body 1, a cover plate 2 arranged at an opening position on the tank body 1 and assembled with the tank body, a feed port 3 and a vertically arranged drive motor 4 arranged on the cover plate, and a stirring shaft 5 arranged in the tank body and connected to the drive motor and vertically arranged. The tank body 1 is provided with a control panel 6 located on the side of the tank body and a discharge port 7 located at the bottom of the tank body. The tank body 1 is provided with a pH meter 8 and a thermometer 9 extending into the tank body and feeding back signals to the control panels respectively.

[0045] A water inlet pipe connected to the tank body after the hot water pipe 10 and the cold water pipe 11 are merged is arranged on the bottom of the tank body 1. The hot water pipe and the cold water pipe are respectively provided with a first temperature sensing valve 12 and a second temperature sensing valve 13. The first temperature sensing valve and the second temperature sensing valve are respectively fed back with the thermometer signal. When the temperature exceeds the set temperature range, the cold water or hot water switch will be automatically turned on to cool down or heat up. When the temperature and pH are kept within a suitable range, the bacterial liquid can be added to ensure that the added strain can survive and maintain good vitality in the high-concentration humic acid liquid.

[0046] The cover plate 2 is provided with a bracket 14 for installing a driving motor; the stirring shaft 5 is provided with a six-blade disc turbine agitator 15 and a three-impeller agitator 16 located in the middle and bottom of the stirring shaft, and the stirring shaft is provided with two different stirring impellers, the upper stirring impeller is a six-blade disc turbine agitator, which stirs radially to reduce resistance; the lower end is a three-impeller agitator, which stirs axially. The two different stirring modes can fully stir and mix the synthetic raw materials to avoid the accumulation of raw materials on the barrel wall or the bottom of the barrel. The pH meter 8 and the thermometer 9 are both provided with stainless steel electrode protective sleeves. The discharge port 7 is provided with a switch 17 for closing or opening the discharge channel; the tank body 1 is provided with a visual window 18 with a volume scale located on the side of the tank body. At least three support legs 19 are provided at the bottom of the tank body on the tank body 1, and the end of the support leg away from the tank body is fixedly connected to the bottom plate.

[0047] Example 2

[0048] The water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing yield of the present invention comprises 20 parts by weight of potassium humate from mineral sources, 25 parts by weight of urea, 10 parts by weight of potassium pyrophosphate, 5 parts by weight of potassium nitrate, 5 parts by weight of potassium dihydrogen phosphate, 5 parts by weight of orthophosphoric acid, 2 parts by weight of Tween 80, 1 part by weight of sodium carboxymethyl cellulose, 2 parts by weight of propylene glycol, 20 parts by weight of Bacillus amyloliquefaciens plant subspecies bacterial body coating liquid, and 60 parts by weight of water;

[0049] Among them, the strain number of Bacillus amyloliquefaciens plantarum is CICC 21168.

[0050] The preparation method of the water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production of the present invention comprises the following steps:

[0051] Step S1: preparing a nutrient broth medium in a fermentation tank, sterilizing it at high temperature and high pressure, and then inoculating it with Bacillus amyloliquefaciens plant subspecies, and fermenting it at 35° C. for 20 hours to obtain a fermentation liquid;

[0052] Step S2: the fermentation liquid in the fermenter in step S1 is centrifuged and dehydrated using a disc centrifuge;

[0053] Step S3: adding Tween 80 and propylene glycol in the above weight ratio to the bacterial sludge, and mixing evenly to make the bacterial cells evenly dispersed to form a bacterial coating liquid;

[0054] Step S4: adding water in the above weight proportion to the preparation device, and sequentially adding potassium humate from mineral sources, urea, potassium pyrophosphate, potassium nitrate, and potassium dihydrogen phosphate in the above weight proportion, sealing, heating and stirring for 40 minutes at a heating temperature of 50°C-70°C, adding orthophosphoric acid in the above weight proportion after stirring, adjusting the pH to 7.5, and cooling to room temperature to obtain a coolant;

[0055] Step S5: adding the above-mentioned sodium carboxymethyl cellulose in the weight proportion to the cooling liquid, and emulsifying it using a high-speed shear emulsifier to obtain an emulsion;

[0056] Step S6: adding the bacterial coating solution obtained in step S3 to the emulsion obtained in step S5, stirring for 10-20 minutes, and obtaining a water-soluble bacterial fertilizer containing humic acid.

[0057] The plant subspecies of Bacillus amyloliquefaciens in step S1 is derived from the School of Food Science and Engineering of China Agricultural University and is currently deposited in the China Industrial Microbiological Culture Collection Center with a deposit number of CICC21168. The strain characteristics are: rod-shaped, G+, forming oval endogenous spores, aerobic, resistant to 1%-7% NaCl concentration of glucose, sucrose, maltose, galactose, trehalose acid production, lactose, fructose, mannitol, raffinose, xylose no acid production, no decomposition of starch, catalase and oxidase positive.

[0058] In the above step S1, the sterilization temperature is 121° C. and the sterilization time is 15 minutes; in step S2, the speed of the disc centrifuge is 1000 r / min; in step S5, the emulsification time of the high-speed shear emulsifier is 20 minutes; and in step S6, the stirring speed of the mixer is 200 r / min.

[0059] The preparation device in the above step S4 includes a tank body 1, a cover plate 2 arranged at an opening position on the tank body 1 and assembled with the tank body, a feed port 3 and a vertically arranged drive motor 4 arranged on the cover plate, and a stirring shaft 5 arranged in the tank body and connected to the drive motor and vertically arranged. The tank body 1 is provided with a control panel 6 located on the side of the tank body and a discharge port 7 located at the bottom of the tank body. The tank body 1 is provided with a pH meter 8 and a thermometer 9 extending into the tank body and feeding back signals to the control panels respectively.

[0060] A water inlet pipe connected to the tank body after the hot water pipe 10 and the cold water pipe 11 are merged is arranged on the bottom of the tank body 1. The hot water pipe and the cold water pipe are respectively provided with a first temperature sensing valve 12 and a second temperature sensing valve 13. The first temperature sensing valve and the second temperature sensing valve are respectively fed back with the thermometer signal. When the temperature exceeds the set temperature range, the cold water or hot water switch will be automatically turned on to cool down or heat up. When the temperature and pH are kept within a suitable range, the bacterial liquid can be added to ensure that the added strain can survive and maintain good vitality in the high-concentration humic acid liquid.

[0061] The cover plate 2 is provided with a bracket 14 for installing a driving motor; the stirring shaft 5 is provided with a six-blade disc turbine agitator 15 and a three-impeller agitator 16 located in the middle and bottom of the stirring shaft, and the stirring shaft is provided with two different stirring impellers, the upper stirring impeller is a six-blade disc turbine agitator, which stirs radially to reduce resistance; the lower end is a three-impeller agitator, which stirs axially. The two different stirring modes can fully stir and mix the synthetic raw materials to avoid the accumulation of raw materials on the barrel wall or the bottom of the barrel. The pH meter 8 and the thermometer 9 are both provided with stainless steel electrode protective sleeves. The discharge port 7 is provided with a switch 17 for closing or opening the discharge channel; the tank body 1 is provided with a visual window 18 with a volume scale located on the side of the tank body. At least three support legs 19 are provided at the bottom of the tank body on the tank body 1, and the end of the support leg away from the tank body is fixedly connected to the bottom plate.

[0062] Example 3

[0063] The humic acid-containing water-soluble bacterial fertilizer for promoting growth and increasing yield comprises 50 parts by weight of mineral source potassium humate, 30 parts by weight of urea, 10 parts by weight of potassium pyrophosphate, 8 parts by weight of potassium nitrate, 8 parts by weight of potassium dihydrogen phosphate, 8 parts by weight of orthophosphoric acid, 5 parts by weight of Tween 80, 3 parts by weight of sodium carboxymethyl cellulose, 5 parts by weight of propylene glycol, 30 parts by weight of bacillus amyloliquefaciens plant subspecies bacterial body coating liquid, and 100 parts by weight of water; wherein the strain number of the bacillus amyloliquefaciens plant subspecies is CICC 21168.

[0064] The invention greatly improves the concentration of mineral source potassium humate through a high temperature-stirring-emulsification process, which is 3-5 times the humic acid concentration of similar products on the market; at the same time, by multi-layer coating of the bacterial body, the bacterial agent can stably survive in a hypertonic solution, filling the gap of water-soluble bacterial fertilizer containing humic acid; the water-soluble bacterial fertilizer containing humic acid prepared by the invention has a small dosage and is easy to use; compared with the control group, the soil enzyme activity can be significantly improved, the development of crop capillary roots can be promoted, and the crop yield and quality can be significantly improved.

[0065] The preparation method of the water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production of the present invention comprises the following steps:

[0066] Step S1: preparing a nutrient broth medium in a fermentation tank, sterilizing it at high temperature and high pressure, and then inoculating it with Bacillus amyloliquefaciens plant subspecies, and fermenting it at 35° C. for 20 hours to obtain a fermentation liquid;

[0067] Step S2: the fermentation liquid in the fermenter in step S1 is centrifuged and dehydrated using a disc centrifuge;

[0068] Step S3: adding Tween 80 and propylene glycol in the above weight ratio to the bacterial sludge, and mixing evenly to make the bacterial cells evenly dispersed to form a bacterial coating liquid;

[0069] Step S4: adding water in the above weight proportion to the preparation device, and sequentially adding potassium humate from mineral sources, urea, potassium pyrophosphate, potassium nitrate, and potassium dihydrogen phosphate in the above weight proportion, sealing, heating and stirring for 40 minutes at a heating temperature of 50°C-70°C, adding orthophosphoric acid in the above weight proportion after stirring, adjusting the pH to 7.5, and cooling to room temperature to obtain a coolant;

[0070] Step S5: adding the above-mentioned sodium carboxymethyl cellulose in the weight proportion to the cooling liquid, and emulsifying it using a high-speed shear emulsifier to obtain an emulsion;

[0071] Step S6: adding the bacterial coating solution obtained in step S3 to the emulsion obtained in step S5, stirring for 10-20 minutes, and obtaining a water-soluble bacterial fertilizer containing humic acid.

[0072] The plant subspecies of Bacillus amyloliquefaciens in step S1 is derived from the School of Food Science and Engineering of China Agricultural University and is currently deposited in the China Industrial Microbiological Culture Collection Center with a deposit number of CICC21168. The strain characteristics are: rod-shaped, G+, forming oval endogenous spores, aerobic, resistant to 1%-7% NaCl concentration of glucose, sucrose, maltose, galactose, trehalose acid production, lactose, fructose, mannitol, raffinose, xylose no acid production, no decomposition of starch, catalase and oxidase positive.

[0073] In the above step S1, the sterilization temperature is 121° C. and the sterilization time is 15 minutes; in step S2, the speed of the disc centrifuge is 1000 r / min; in step S5, the emulsification time of the high-speed shear emulsifier is 20 minutes; and in step S6, the stirring speed of the mixer is 200 r / min.

[0074] The preparation device in the above step S4 includes a tank body 1, a cover plate 2 arranged at an opening position on the tank body 1 and assembled with the tank body, a feed port 3 and a vertically arranged drive motor 4 arranged on the cover plate, and a stirring shaft 5 arranged in the tank body and connected to the drive motor and vertically arranged. The tank body 1 is provided with a control panel 6 located on the side of the tank body and a discharge port 7 located at the bottom of the tank body. The tank body 1 is provided with a pH meter 8 and a thermometer 9 extending into the tank body and feeding back signals to the control panels respectively.

[0075] A water inlet pipe connected to the tank body after the hot water pipe 10 and the cold water pipe 11 are merged is arranged on the bottom of the tank body 1. The hot water pipe and the cold water pipe are respectively provided with a first temperature sensing valve 12 and a second temperature sensing valve 13. The first temperature sensing valve and the second temperature sensing valve are respectively fed back with the thermometer signal. When the temperature exceeds the set temperature range, the cold water or hot water switch will be automatically turned on to cool down or heat up. When the temperature and pH are kept within a suitable range, the bacterial liquid can be added to ensure that the added strain can survive and maintain good vitality in the high-concentration humic acid liquid.

[0076] The cover plate 2 is provided with a bracket 14 for installing a driving motor; the stirring shaft 5 is provided with a six-blade disc turbine agitator 15 and a three-impeller agitator 16 located in the middle and bottom of the stirring shaft, and the stirring shaft is provided with two different stirring impellers, the upper stirring impeller is a six-blade disc turbine agitator, which stirs radially to reduce resistance; the lower end is a three-impeller agitator, which stirs axially. The two different stirring modes can fully stir and mix the synthetic raw materials to avoid the accumulation of raw materials on the barrel wall or the bottom of the barrel. The pH meter 8 and the thermometer 9 are both provided with stainless steel electrode protective sleeves. The discharge port 7 is provided with a switch 17 for closing or opening the discharge channel; the tank body 1 is provided with a visual window 18 with a volume scale located on the side of the tank body. At least three support legs 19 are provided at the bottom of the tank body on the tank body 1, and the end of the support leg away from the tank body is fixedly connected to the bottom plate.

[0077] Application Examples

[0078] The high-concentration humic acid-containing water-soluble bacterial fertilizer produced in Example 2 was subjected to different treatments to test its growth-promoting and yield-increasing effects on peppers.

[0079] 1. Overview of the test site

[0080] The experiment was set up at the pepper planting base in Wuxian Town, Tongan District. The previous crop was pepper, the soil texture was paddy soil, and the basic physical and chemical properties of the soil (20cm) are as follows:

[0081]

[0082] 2. Test materials

[0083] Test crop: Yellow pepper

[0084] High-concentration humic acid-containing water-soluble fertilizer: humic acid 100g / L, macroelements (100-30-100), effective live bacteria count ≥ 6.5 billion / ml; High-concentration humic acid-containing water-soluble fertilizer inactivated group (121℃, 15 minutes): humic acid 100g / L, macroelements (100-30-100), effective live bacteria count 0;

[0085] Fertilizers tested: Lion Horse compound fertilizer (15-15-15), natural water-soluble fertilizer (15-5-30).

[0086] 3. Test methods

[0087] 3.1 Experimental design

[0088] The experiment adopted random block arrangement, with 5 treatments in total, namely

[0089] T1: conventional fertilization + clean water;

[0090] T2: conventional fertilization + high concentration of humic acid-containing water-soluble fertilizer inactivated group (foliar spray);

[0091] T3: conventional fertilization + high concentration of humic acid-containing water-soluble fertilizer inactivated group (root irrigation);

[0092] T4: conventional fertilization + high concentration of humic acid-containing water-soluble fertilizer (foliar spray);

[0093] T5: Conventional fertilization + high-concentration water-soluble fertilizer containing humic acid (root irrigation).

[0094] Each plot was repeated three times, with a total of 15 plots. Ridge cultivation was adopted, with a ridge length of 3 meters and a ridge width of 0.65 meters. Double rows were planted in each ridge, with a row spacing of 0.50m and a plant spacing of 0.25m. The planting mode was film-covered cultivation.

[0095] 3.2 Fertilizer application amount and management method

[0096] The first application of the test was carried out on February 19, 2024; the second application was carried out on February 26; the third application was carried out on March 2; the test ended and sampling was carried out on March 7.

[0097] The application amount was 300 times dilution of high-concentration humic acid-containing water-soluble fertilizer and inactivated group, and the root irrigation amount was 100ml / plant. During the experiment, conventional fertilization was applied twice, each time with 10kg / mu of Lion Horse compound fertilizer and 5kg / mu of natural water-soluble fertilizer. Other field management measures were the same as conventional cultivation.

[0098] 3.3 Sample collection and processing

[0099] 3.3.1 Soil nutrient determination

[0100] After the experiment, the five-point soil sampling method was used to determine soil sucrase, urease activity, phosphatase, catalase activity, soil pH, available potassium, available phosphorus, alkaline nitrogen, organic matter content and other indicators using common laboratory methods.

[0101] 3.3.2 Plant determination method

[0102] Five representative pepper plants were selected from each plot, and 2-3 cm long peppers were selected from each plant for hanging. The length was measured before each fertilization, and developmental indicators such as fresh weight and quality were determined after the experiment.

[0103] 4. Pepper plant related traits

[0104]

[0105] 5. Soil physical and chemical indicators

[0106]

[0107] 6. Soil enzyme activity

[0108] The above-mentioned embodiments only express several implementation modes of the present invention, and the description thereof is relatively specific and detailed.

[0109] However, this should not be construed as limiting the scope of the invention patent. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the invention, and these all fall within the protection scope of the invention. Therefore, the protection scope of the invention patent shall be subject to the attached claims.

Claims

1. A water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing production, characterized in that: The water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing yield comprises 15-50 parts by weight of potassium humate from mineral source, 10-30 parts by weight of urea, 3-10 parts by weight of potassium pyrophosphate, 2-8 parts by weight of potassium nitrate, 2-8 parts by weight of potassium dihydrogen phosphate, 2-8 parts by weight of orthophosphoric acid, 0.5-5 parts by weight of Tween 80, 0.5-3 parts by weight of sodium carboxymethyl cellulose, 1-5 parts by weight of propylene glycol, 10-30 parts by weight of Bacillus amyloliquefaciens plant subspecies bacterial coating liquid, and 50-100 parts by weight of water; Among them, the strain number of Bacillus amyloliquefaciens plantarum is CICC 21168.

2. The water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production as claimed in claim 1, characterized in that: The water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing yield comprises 20-40 parts by weight of potassium humate from mineral source, 15-20 parts by weight of urea, 5-8 parts by weight of potassium pyrophosphate, 4-5 parts by weight of potassium nitrate, 3-6 parts by weight of potassium dihydrogen phosphate, 2-6 parts by weight of orthophosphoric acid, 1-3 parts by weight of Tween 80, 0.5-1 parts by weight of sodium carboxymethyl cellulose, 1-3 parts by weight of propylene glycol, 15-25 parts by weight of Bacillus amyloliquefaciens plant subspecies bacterial coating liquid, and 50-70 parts by weight of water; Among them, the strain number of Bacillus amyloliquefaciens plantarum is CICC 21168.

3. The water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production as claimed in claim 1, characterized in that: The water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing yield comprises 20 parts by weight of mineral source potassium humate, 25 parts by weight of urea, 10 parts by weight of potassium pyrophosphate, 5 parts by weight of potassium nitrate, 5 parts by weight of potassium dihydrogen phosphate, 5 parts by weight of orthophosphoric acid, 2 parts by weight of Tween 80, 1 part by weight of sodium carboxymethyl cellulose, 2 parts by weight of propylene glycol, 20 parts by weight of Bacillus amyloliquefaciens plant subspecies bacterial coating liquid, and 60 parts by weight of water; Among them, the strain number of Bacillus amyloliquefaciens plantarum is CICC 21168.

4. A method for preparing a water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing production, which is used to prepare the water-soluble bacterial fertilizer containing humic acid for promoting growth and increasing production as claimed in any one of claims 1 to 3, characterized in that The following steps are involved: Step S1: preparing a nutrient broth medium in a fermentation tank, sterilizing it at high temperature and high pressure, and then inoculating it with Bacillus amyloliquefaciens plant subspecies, and fermenting it at 35° C. for 20 hours to obtain a fermentation liquid; Step S2: the fermentation liquid in the fermenter in step S1 is centrifuged and dehydrated using a disc centrifuge to obtain bacterial sludge; Step S3: adding Tween 80 and propylene glycol in the above weight ratio to the bacterial sludge, and mixing evenly to make the bacterial cells evenly dispersed to form a bacterial coating liquid; Step S4: adding water in the above weight proportion to the preparation device, and sequentially adding potassium humate from mineral sources, urea, potassium pyrophosphate, potassium nitrate, and potassium dihydrogen phosphate in the above weight proportion, sealing, heating and stirring for 40 minutes at a heating temperature of 50°C-70°C, adding orthophosphoric acid in the above weight proportion after stirring, adjusting the pH to 7.5, and cooling to room temperature to obtain a coolant; Step S5: adding the above-mentioned sodium carboxymethyl cellulose in the weight proportion to the cooling liquid, and emulsifying it using a high-speed shear emulsifier to obtain an emulsion; Step S6: adding the bacterial coating solution obtained in step S3 to the emulsion obtained in step S5, stirring for 10-20 minutes, and obtaining a water-soluble bacterial fertilizer containing humic acid.

5. The method for preparing a water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production as claimed in claim 4, characterized in that: The Bacillus amyloliquefaciens plant subspecies in step S1 is derived from the School of Food Science and Engineering of China Agricultural University and is deposited in the China Industrial Microbiological Culture Collection Center with a deposit number of CICC21168.

6. The method for preparing a water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production as claimed in claim 4, characterized in that: In step S1, the sterilization temperature is 121° C. and the sterilization time is 15 minutes; in step S2, the rotation speed of the disc centrifuge is 1000 r / min.

7. The method for preparing a water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production as claimed in claim 4, characterized in that: In step S5, the emulsification time of the high-speed shear emulsifier is 20 minutes.

8. The method for preparing a water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production as claimed in claim 4, characterized in that: In step S6, the stirring speed of the stirrer is 200 r / min.

9. The method for preparing a water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production as claimed in claim 4, characterized in that: The preparation device in step S4 includes a tank body, a cover plate arranged at an opening position on the tank body and assembled with the tank body, a feed port arranged on the cover plate and a vertically arranged driving motor, a stirring shaft arranged in the tank body and connected to the driving motor and vertically arranged, the tank body is provided with a control panel located on the side of the tank body and a discharge port located at the bottom of the tank body, and the tank body is provided with a pH meter and a thermometer extending into the tank body and feeding back signals to the control panels respectively; A water inlet pipe connected to the tank body after the hot water pipe and the cold water pipe merge is arranged on the bottom of the tank body, and a first temperature sensing valve and a second temperature sensing valve are arranged on the hot water pipe and the cold water pipe respectively, and the first temperature sensing valve and the second temperature sensing valve respectively feedback the thermometer signal.

10. The method for preparing a water-soluble bacterial fertilizer containing humic acid for promoting plant growth and increasing production as claimed in claim 9, characterized in that: The cover plate is provided with a bracket for mounting the driving motor; the stirring shaft is provided with a six-blade disc turbine stirrer and a three-impeller stirrer located in the middle and bottom of the stirring shaft; the pH meter and the thermometer are both provided with stainless steel electrode protective sleeves; The discharge port is provided with a switch for closing or opening the discharge channel; the tank body is provided with a visual window with a volume scale on the side of the tank body; At least three supporting legs are arranged on the bottom of the tank body, and one end of the supporting legs away from the tank body is fixedly connected to the bottom plate.

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