Production device of cordyceps polysaccharide nutrition immune growth promoting agent

Abstract

The invention relates to the technical field of polysaccharide nutritional agent production, and discloses a cordyceps polysaccharide nutrition immune growth promoting agent production device which comprises an incubator, an adjusting mechanism is fixedly connected to the left end of the top of the incubator, a control mechanism is arranged in the adjusting mechanism, and a stirring mechanism is fixedly connected to the interior of the incubator; the adjusting mechanism comprises a material box, the material box is fixedly connected to the left end of the top of the culture box, a material control round bar is arranged at the bottom end in the material box, a long rod is fixedly connected to the middle in the material control round bar, and a round cover plate is fixedly connected to the interior of the top end of the material box. According to the production device of the cordyceps polysaccharide nutrition immune growth promoting agent, the production performance of aquatic animals can be improved, the immunity, oxidation resistance and stress resistance of the aquatic animals are improved and the intestinal health is improved by utilizing various immune regulation mechanisms of cordyceps polysaccharide, so that novel feed and feed resources are developed; and the recycling effect is realized.

Description

Technical Field

[0001] The invention relates to the technical field of polysaccharide nutrient production, in particular to a production device for a cordyceps polysaccharide nutrient immune growth-promoting agent. Background Art

[0002] Traditional Chinese medicine fermentation is one of the important ways to develop and utilize traditional Chinese medicine resources. According to current research, the fermented waste materials of traditional Chinese medicine are rich in nutrients and contain a variety of effective medicinal ingredients. They still have high application value in the breeding industry. With the rapid development of my country's aquaculture industry, various antibiotics and chemical drugs are used more and more widely in aquaculture. Their use destroys the balance of the microecology in animals, leading to the production of drug-resistant strains, residues in livestock products, a wide spectrum of drug resistance, and other problems, which reduces animal immunity. Finding alternatives to antibiotics has become an inevitable trend in the development of aquaculture.

[0003] In addition, studies have found that Cordyceps sinensis has a wide range of biological activities, including anti-inflammatory, antibacterial, antioxidant, anti-tumor, liver protection and immunomodulatory effects. Modern medical clinical studies have found that Cordyceps sinensis can separate a variety of beneficial bioactive substances, mainly including cordycepic acid, cordyceps polysaccharides, fat, protein. In addition, it also contains vitamin B12, ergosterol, hexose alcohol, alkaloids, etc. Therefore, Cordyceps sinensis can be cultured accordingly and the bioactive substances can be extracted. Summary of the invention

[0004] The purpose of the present invention is to provide a production device for a Cordyceps polysaccharide nutritional immune growth-promoting agent to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a production device of a Cordyceps polysaccharide nutritional immune growth-promoting agent, comprising an incubator, wherein the left end of the top of the incubator is fixedly connected to an adjustment mechanism, a control mechanism is arranged inside the adjustment mechanism, and a stirring mechanism is fixedly connected inside the incubator;

[0006] The adjustment mechanism includes a material box, which is fixedly connected to the left end of the top of the incubator, a material control round bar is provided at the bottom end of the material box, a long rod is fixedly connected to the middle of the material control round bar, a round cover plate is fixedly connected to the top of the material box, a support arc seat is fixedly connected to the top of the round cover plate, a first spring is fixedly connected to the top of the support arc seat, a T-shaped rod is fixedly connected to the top of the first spring, and an arc plate is fixedly connected to the bottom end of the support arc seat.

[0007] Preferably, through holes are provided inside the support arc seat and the arc plate, and the surface of the T-shaped rod passes through the through holes provided inside the support arc seat and the arc plate, so that the T-shaped rod can perform a supporting function in the through holes provided inside the support arc seat and the arc plate, thereby facilitating the rotation of the T-shaped rod.

[0008] Preferably, a first circular groove is formed in equidistant sections in the middle of the surface of the material control circular strip, and a second circular groove is formed in equidistant sections at the bottom of the material box.

[0009] Preferably, a material port is provided inside the circular cover plate, a slide groove is provided inside the circular cover plate, and the surface of the arc plate is slidably connected to the inside of the slide groove, so that the arc plate can be rotated under the limit inside the slide groove, thereby facilitating material control.

[0010] Preferably, the control mechanism includes a support seat, the support seat is fixedly connected to the top of the circular cover plate, a T-shaped ring is slidably connected inside the support seat, a second spring is fixedly connected inside the T-shaped ring, one end of the second spring is fixedly connected to a limiting plate, a T-shaped rod is fixedly connected inside the limiting plate, one end of the control mechanism is fixedly connected to a clamping strip, and a clamping plate is fixedly connected to the surface of the support seat.

[0011] Preferably, a slide groove is provided inside the support seat, and the surface of the limiting plate is slidably connected to the inside of the slide groove, so that the limiting plate can slide under the limitation inside the slide groove.

[0012] Preferably, the surface of the clamping plate is provided with tooth grooves, and the surface of the clamping strip is provided with teeth.

[0013] Preferably, the stirring mechanism includes a motor, the motor is fixedly connected to the top of the incubator, the bottom end of the motor is fixedly connected to an electric pole, the surface of the electric pole is fixedly connected to a stirring blade, the top end of the surface of the electric pole is rotatably connected to a round frame, the bottom end of the round frame is rotatably connected to a branch rod, and the surface of the branch rod is fixedly connected to a branch stirring rod.

[0014] Preferably, a through hole is provided inside the circular frame, and the surface of the electric pole passes through the through hole provided inside the circular frame, so that the electric pole can be easily rotated under the action of the through hole.

[0015] Compared with the prior art, the present invention provides a production device for a Cordyceps polysaccharide nutritional immune growth-promoting agent, which has the following beneficial effects:

[0016] 1. The production device of the Cordyceps polysaccharide nutritional immune growth promoter, through the provided adjustment mechanism, when it is necessary to control the feed, the T-shaped rod is pulled upward and separated from the inside of the arc plate, and then the T-shaped rod is rotated to drive the arc plate to rotate, and then the feed opening inside the feed box can be controlled, thereby preventing external dust and the like from entering the feed box and affecting the production of the Cordyceps polysaccharide nutritional immune growth promoter. In this way, the multiple immune regulation mechanisms of Cordyceps polysaccharides can be used to improve the production performance of aquatic animals, improve the immunity, antioxidant, and anti-stress capabilities of aquatic animals, and improve intestinal health, thereby achieving the effect of developing new feeds and feed resources and realizing their resource utilization.

[0017] 2. The production device of the Cordyceps polysaccharide nutritional immune growth promoter has a control mechanism set up, and the T-shaped rod drives the card bar to move, so that the card bar is separated from the card plate surface, and then the long rod is moved up and down, thereby driving the relative movement between the material control round bar and the material box, so that the material control can be carried out by utilizing the dislocation movement of the circular groove 1 and the circular groove 2.

[0018] 3. The production device of the cordyceps polysaccharide nutritional immune growth promoter stirs the inside of the incubator through the stirring mechanism and the stirring blades. During the stirring process, the circular frame is rotated to drive the sub-rods and the sub-stirring rods to stir, thereby speeding up the stirring of the culture mixture inside the incubator, and further promoting the production of the cordyceps polysaccharide nutritional immune growth promoter. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative labor:

[0020] Figure 1 It is a three-dimensional diagram of the structure of the present invention;

[0021] Figure 2 It is a three-dimensional split cross-sectional view of the structural adjustment mechanism of the present invention;

[0022] Figure 3 This is a three-dimensional exploded view of the parts of the structural adjustment mechanism of the present invention;

[0023] Figure 4 It is a three-dimensional cross-sectional view of the stirring mechanism of the structure of the present invention;

[0024] Figure 5 This is a three-dimensional exploded view of the structural control mechanism of the present invention;

[0025] Figure 6 This is a three-dimensional exploded view of the stirring mechanism of the present invention;

[0026] Figure 7 Schematic diagram of the absorbance curve of the structure of the present invention.

[0027] In the figure: 1. incubator; 2. adjustment mechanism; 21. material box; 22. material control round bar; 23. long rod; 24. round cover plate; 25. support arc seat; 26. first spring; 27. T-shaped rod; 28. arc plate; 3. control mechanism; 31. support seat; 32. T-shaped ring; 33. second spring; 34. limit plate; 35. T-shaped rod; 36. clamping bar; 37. clamping plate; 4. stirring mechanism; 41. motor; 42. electric pole; 43. stirring blade; 44. sub-rod; 45. sub-stirring rod; 46. round frame. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] The present invention provides the following technical solutions:

[0031] Embodiment 1

[0032] Combination Figures 1 to 6 , a production device of a Cordyceps polysaccharide nutritional immune growth-promoting agent, comprising an incubator 1, a regulating mechanism 2 is fixedly connected to the left end of the top of the incubator 1, a control mechanism 3 is arranged inside the regulating mechanism 2, and a stirring mechanism 4 is fixedly connected inside the incubator 1;

[0033] The adjustment mechanism 2 includes a material box 21, which is fixedly connected to the left end of the top of the incubator 1. A material control round bar 22 is provided at the bottom end of the material box 21, and a long rod 23 is fixedly connected to the middle of the material control round bar 22. A circular cover plate 24 is fixedly connected to the top of the material box 21, and a support arc seat 25 is fixedly connected to the top of the circular cover plate 24. A first spring 26 is fixedly connected to the top of the support arc seat 25, and a T-shaped rod 27 is fixedly connected to the top of the first spring 26. An arc plate 28 is fixedly connected to the bottom end of the support arc seat 25. Through holes are provided in the support arc seat 25 and the arc plate 28. The surface of the T-shaped rod 27 runs through the through holes in the support arc seat 25 and the arc plate 28. A circular groove 1 is equidistantly provided in the middle of the surface of the material control round bar 22, and a circular groove 2 is equidistantly provided at the bottom end of the material box 21.

[0034] Furthermore, a material opening is opened inside the circular cover plate 24, a slide groove is opened inside the circular cover plate 24, and the surface of the arc plate 28 is slidably connected to the inside of the slide groove. When material control is required, the T-shaped rod 27 is pulled upward to separate from the inside of the arc plate 28, and then the T-shaped rod 27 is rotated to drive the arc plate 28 to rotate, thereby controlling the material opening inside the material box 21, thereby preventing external dust from entering the material box 21 and affecting the production of the Cordyceps polysaccharide nutritional immune growth promoter.

[0035] Embodiment 2

[0036] See also Figure 1-6 On the basis of the first embodiment, the control mechanism 3 further comprises a support seat 31, the support seat 31 is fixedly connected to the top of the circular cover plate 24, a T-shaped ring 32 is slidably connected inside the support seat 31, a second spring 33 is fixedly connected inside the T-shaped ring 32, one end of the second spring 33 is fixedly connected to a limiting plate 34, a T-shaped rod 35 is fixedly connected inside the limiting plate 34, a clamping strip 36 is fixedly connected to one end of the control mechanism 3, a clamping plate 37 is fixedly connected to the surface of the support seat 31, a slide groove is provided inside the support seat 31, and the surface of the limiting plate 34 is slidably connected to the inside of the slide groove.

[0037] Furthermore, a tooth groove is provided on the surface of the card plate 37, and teeth are provided on the surface of the card strip 36. The T-shaped rod 35 drives the card strip 36 to move, thereby allowing the card strip 36 to be separated from the card plate 37 surface. The long rod 23 is then moved up and down, and then the material control circular bar 22 and the material box 21 are driven to move relative to each other, so that the offset movement of the circular groove 1 and the circular groove 2 can be used to control the material.

[0038] Embodiment 3

[0039] See also Figure 1-7On the basis of the first embodiment, the stirring mechanism 4 further comprises a motor 41, the motor 41 is fixedly connected to the top of the incubator 1, the bottom end of the motor 41 is fixedly connected to an electric pole 42, the surface of the electric pole 42 is fixedly connected to a stirring blade 43, the top end of the surface of the electric pole 42 is rotatably connected to a round frame 46, the bottom end of the round frame 46 is rotatably connected to a branch rod 44, and the surface of the branch rod 44 is fixedly connected to a branch stirring rod 45.

[0040] Furthermore, a through hole is opened inside the circular frame 46, and the surface of the electric pole 42 passes through the through hole opened inside the circular frame 46. The stirring blade 43 stirs the inside of the incubator 1. During the stirring process, the circular frame 46 rotates to drive the branch rod 44 and the branch stirring rod 45 to stir, thereby speeding up the stirring of the culture mixture inside the incubator 1, and further promoting the production of Cordyceps polysaccharide nutritional immune growth promoter.

[0041] 1. Determination of polysaccharide content in Cordyceps polysaccharide

[0042] Determination of polysaccharide content of Cordyceps polysaccharide by phenol-sulfuric acid method

[0043] Drawing of glucose standard curve: Take 0.0, 0.2, 0.4, 0.6, 0.8, 1.0 mL of 100 μg / mL standard glucose solution in test tubes, add distilled water to 1 mL. Add 0.5 mL of 6% phenol to each tube, quickly add 2.5 mL of concentrated sulfuric acid (vertically add to avoid contact with the test tube wall, so as to fully mix with the reaction solution), let stand for 10 minutes, vortex shake, 30℃ water bath for 20 minutes, measure the absorbance at 490nm wavelength, and draw the standard curve with the absorbance as the ordinate and the glucose concentration in the corresponding test tube as the abscissa.

[0044] Determination of samples: The sample standard curve is drawn with the glucose standard curve. Three parallel groups of samples need to be set up, and the absorbance values ​​of the three groups of samples are measured, and the average value is recorded as A1.

[0045] The three parallel values ​​of Cordyceps polysaccharide were 0.554, 0.549, 0.556, A1 = 0.553, and the Cordyceps polysaccharide content was 43.11%

[0046] 2. Determination of Antioxidant Capacity of Cordyceps Polysaccharides

[0047] Free radicals can cause damage to the body in three main ways: 1. Destroy cell membranes; 2. Inactivate serum antiproteases; 3. Damage genes, leading to the occurrence and accumulation of cell mutations.

[0048] 2.1 Inhibition of hydroxyl radicals (OH-)

[0049] If biological molecules such as proteins, nucleic acids, and lipids in the body are affected by hydroxyl free radicals, the structure and function of the cells will be damaged, leading to metabolic disorders in the body and causing diseases. The ability to inhibit hydroxyl free radicals is one of the important indicators of the antioxidant capacity of a sample, and is widely used in the research of antioxidant health products and drugs. The most common chemical reaction that produces hydroxyl free radicals is the Fenton reaction. The amount of hydrogen peroxide is proportional to the number of hydroxyl groups produced in the Fenton reaction. When an electron acceptor is added and a color development reaction is carried out using a Griess color developer, a red substance is generated, and its color is proportional to the number of hydroxyl groups. The test is divided into control tubes, measurement tubes, standard tubes, and blank tubes. The experimental operation is carried out strictly in accordance with the operating instructions. The calculation formula is as follows:

[0050] Hydroxyl free radical inhibition ability (U / mL) = (A control - A test) / (A standard - A blank) × C standard × 1 / V sample × N

[0051] Where C standard is 8.824mmol / L, Vsample: sampling volume, N: dilution multiple of the sample before testing.

[0052] Test results: The ability of Cordyceps polysaccharide to inhibit hydroxyl free radicals is: 93.79 (U / mL)

[0053] 2.2 Inhibition of DPPH free radicals

[0054] DPPH is widely used to quantify the antioxidant capacity of biological samples and foods. The DPPH free radical has a single electron and a strong absorption peak at 517nm. Its alcohol solution is purple. If a free radical scavenger is present, the color disappears because the single electron is paired with it. The lighter the color, the lower the A value. The experimental operation was carried out strictly according to the operating instructions. The calculation formula is as follows:

[0055] DPPH free radical scavenging rate (%) = (1-(A measurement-A control) ÷ A blank) × 100%

[0056] Substituting it into the standard curve, the result is: the ability of Cordyceps polysaccharide to inhibit DPPH free radical is: 43 (μgtrolox / mL)

[0057] 2.3 Ability to inhibit superoxide anion free radicals

[0058] The reaction system of xanthine and xanthine oxidase in the body is simulated to produce superoxide anion free radicals. Electron transfer substances and Griess's color developer are added to make the reaction system appear purple-red. The absorbance can be measured by a spectrophotometer. When the sample to be tested contains superoxide anion free radical inhibitors, the absorbance of the measuring tube during colorimetry is lower than that of the control tube. If the sample to be tested contains substances that produce superoxide anion free radicals, the absorbance of the measuring tube during colorimetry is higher than that of the control tube. By using vitamin C as a standard, the ability of the tested item to affect superoxide anion free radicals can be calculated.

[0059] Test results: The ability of Cordyceps polysaccharide to inhibit superoxide anion free radicals is: 288 (U / L)

[0060] In actual operation, when this device is used, when producing the Cordyceps polysaccharide nutritional immune growth-promoting agent, the required raw materials are put into the material box 21 and fall into the culture box 1 for culture and production. When material control is required, the T-shaped rod 27 is pulled upward to separate from the arc plate 28, and then the T-shaped rod 27 is rotated to drive the arc plate 28 to rotate, so as to control the material opening inside the material box 21, thereby preventing external dust from entering the material box 21 and affecting the production of the Cordyceps polysaccharide nutritional immune growth-promoting agent;

[0061] When it is necessary to control the feeding of raw materials, the T-shaped rod 35 is pulled open to drive the clamping strip 36 to move, so that the clamping strip 36 is separated from the clamping position on the surface of the clamping plate 37, and then the long rod 23 is moved up and down, thereby driving the material control round bar 22 and the material box 21 to move relative to each other, so that the material control can be carried out by utilizing the dislocation movement of the round groove 1 and the round groove 2;

[0062] In addition, during cultivation, the motor 41 drives the electric rod 42 to rotate, which in turn drives the stirring blades 43 to stir the inside of the culture box 1. During the stirring process, the circular frame 46 is rotated to drive the sub-rods 44 and the sub-stirring rods 45 to stir, thereby speeding up the stirring of the culture mixture inside the culture box 1, thereby promoting the production of Cordyceps polysaccharide nutritional immune growth promoter.

[0063] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the statement "comprise a ..." do not exclude the presence of other identical elements in the process, method, article or device including the elements.

Claims

1. A production device for a Cordyceps polysaccharide nutritional immune growth-promoting agent, comprising an incubator (1), characterized in that: The left end of the top of the incubator (1) is fixedly connected to an adjusting mechanism (2), a control mechanism (3) is arranged inside the adjusting mechanism (2), and a stirring mechanism (4) is fixedly connected inside the incubator (1); The regulating mechanism (2) comprises a material box (21), the material box (21) being fixedly connected to the left end of the top of the incubator (1), a material control round bar (22) being arranged at the bottom of the material box (21), a long rod (23) being fixedly connected in the middle of the material control round bar (22), a round cover plate (24) being fixedly connected to the top of the material box (21), a support arc seat (25) being fixedly connected to the top of the round cover plate (24), a first spring (26) being fixedly connected to the top of the support arc seat (25), a T-shaped rod (27) being fixedly connected to the top of the first spring (26), and an arc plate (28) being fixedly connected to the bottom of the support arc seat (25).

2. The production device of a Cordyceps polysaccharide nutritional immune growth-promoting agent according to claim 1, characterized in that: Through holes are provided inside the supporting arc seat (25) and the arc plate (28), and the surface of the T-shaped rod (27) penetrates through the through holes provided inside the supporting arc seat (25) and the arc plate (28).

3. The production device of a Cordyceps polysaccharide nutritional immune growth-promoting agent according to claim 2, characterized in that: The middle of the surface of the material control round bar (22) is provided with circular grooves one at equal intervals and in sections, and the bottom of the inner part of the material box (21) is provided with circular grooves two at equal intervals and in sections.

4. The production device of a Cordyceps polysaccharide nutritional immune growth-promoting agent according to claim 3, characterized in that: A material opening is provided inside the circular cover plate (24), a slide groove is provided inside the circular cover plate (24), and the surface of the arc plate (28) is slidably connected to the inside of the slide groove.

5. The production device of a Cordyceps polysaccharide nutritional immune growth-promoting agent according to claim 4, characterized in that: The control mechanism (3) comprises a support seat (31), the support seat (31) is fixedly connected to the top of the circular cover plate (24), a T-shaped ring (32) is slidably connected inside the support seat (31), a second spring (33) is fixedly connected inside the T-shaped ring (32), one end of the second spring (33) is fixedly connected to a limiting plate (34), a T-shaped rod (35) is fixedly connected inside the limiting plate (34), one end of the control mechanism (3) is fixedly connected to a clamping strip (36), and a clamping plate (37) is fixedly connected to the surface of the support seat (31).

6. The production device of a Cordyceps polysaccharide nutritional immune growth-promoting agent according to claim 5, characterized in that: A sliding groove is provided inside the support seat (31), and the surface of the limiting plate (34) is slidably connected to the inside of the sliding groove.

7. The production device of a Cordyceps polysaccharide nutritional immune growth-promoting agent according to claim 6, characterized in that: The surface of the clamping plate (37) is provided with tooth grooves, and the surface of the clamping strip (36) is provided with teeth.

8. The production device of the Cordyceps polysaccharide nutritional immune growth-promoting agent according to claim 7, characterized in that: The stirring mechanism (4) comprises a motor (41), the motor (41) is fixedly connected to the top of the incubator (1), the bottom end of the motor (41) is fixedly connected to an electric pole (42), the surface of the electric pole (42) is fixedly connected to a stirring blade (43), the top end of the surface of the electric pole (42) is rotatably connected to a round frame (46), the bottom end of the round frame (46) is rotatably connected to a branch rod (44), and the surface of the branch rod (44) is fixedly connected to a branch stirring rod (45).

9. The production device of a Cordyceps polysaccharide nutritional immune growth-promoting agent according to claim 8, characterized in that: A through hole is provided inside the circular frame (46), and the surface of the electric pole (42) passes through the through hole provided inside the circular frame (46).

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