Treatment method for improving seed germination rate of eragrostis pilosa

Abstract

The invention discloses a treatment method for improving the germination rate of eragrostis melanchoides seeds, and relates to the technical field of agricultural planting, and the treatment method comprises the following steps: collecting eragrostis melanchoides seeds, removing receptacles and seed hairs, drying the collected eragrostis melanchoides seeds by using an air blower, treating the seeds, and soaking the seeds with a hydrogen peroxide solution. According to the treatment method for improving the germination rate of the eragrostis black spike seeds, preliminary separation of receptacles and the seeds can be achieved through screening; removing impurities and receptacles, and finally removing seed hair by friction with a friction machine; after the seed hair is removed, the seeds are easier to contact with soil during sowing, so that moisture and nutrients in the soil can be absorbed; the pathogenic bacteria can be killed by soaking the seeds with hydrogen peroxide, so that the probability that the seeds are infected by diseases in the germination process is reduced; for some seeds with seed coats having certain hardness or poor permeability, the oxidation effect of hydrogen peroxide can soften the seed coats, so that the permeability of the seed coats is increased.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural planting, and in particular to a processing method for improving the germination rate of teff seeds. Background Art

[0002] With the increasing demand for ecological protection and agricultural development, the research and utilization of wild plant resources have received more and more attention. As a plant with potential value, the cultivation of seeds of Eragrostis nigra is of great significance to the reproduction and ecological restoration of the species.

[0003] However, there are some problems with black teff seeds in their natural state, which limit their germination and growth. On the one hand, after the seeds are collected, they often carry receptacles and seed hairs, which affect the purity of the seeds and the sowing effect, and increase the risk of mold and rot during seed storage. On the other hand, the seeds may carry pathogens and are easily infected by diseases during the germination process. In addition, the seed coat of some seeds is hard or has poor permeability, which is not conducive to the absorption of water and oxygen, resulting in germination difficulties. In addition, the lack of suitable cultivation conditions also makes the germination rate of black teff seeds low, and the seedlings are not strong enough to adapt to the complex natural environment.

[0004] In order to solve these problems, the present invention proposes a method for cultivating black teff seeds. Through a series of carefully designed steps, including removing the receptacle and seed hairs, treating the seeds with a specific solution, using a suitable culture medium, and cultivating in a light incubator, the method aims to improve the germination rate of black teff seeds, promote the robust growth of seedlings, enhance their stress resistance, and provide technical support for the large-scale reproduction and ecological application of black teff. Summary of the invention

[0005] To achieve the above object, the present invention is implemented by the following technical scheme: a treatment method for improving the germination rate of black stigma seeds, comprising the following steps:

[0006] Step 1: Collect black teff seeds;

[0007] Step 2, removing the receptacle and seed hairs, and using a blower to dry the collected black spike teff seeds. The moisture content of the dried seeds is reduced, which can reduce the risk of mildew and rot during storage and extend the shelf life of the seeds. The air-dried seeds are then sieved; sieving can achieve preliminary separation of the receptacle and the seeds; impurities and the receptacle are removed, and finally the seed hairs are rubbed off with a friction machine; after the seed hairs are removed, the seeds are more likely to contact with the soil during sowing, which is beneficial to absorbing moisture and nutrients in the soil.

[0008] Step 3: Seed treatment: soaking the seeds in hydrogen peroxide solution;

[0009] Soaking in hydrogen peroxide can kill these pathogens and reduce the chance of seeds being infected by diseases during the germination process; for some seeds with a certain hardness or poor permeability in the seed coat, the oxidizing effect of hydrogen peroxide can soften the seed coat and increase its permeability.

[0010] Step 4: Rinse the seeds soaked in the hydrogen peroxide solution with water;

[0011] Step 5: Soak the rinsed seeds in a gibberellin solution to obtain seeds treated with gibberellin; gibberellin promotes the growth of stems and leaves of seedlings. After the seeds soaked in the gibberellin solution germinate, the elongation of the stem internodes of the seedlings accelerates.

[0012] Step 6: Place the seeds treated with the gibberellin solution in a culture dish containing a preset culture medium;

[0013] Step 7: Place the seeds treated with the gibberellin solution in a light incubator for cultivation to obtain the finally cultivated black stigma seeds. Light can provide energy for seed germination and promote seed germination.

[0014] Preferably, the friction wheel of the friction machine in step 2 rotates at 100-300 revolutions per minute, the friction pressure of the friction wheel may be 0.1-0.3 kg / cm2, and the friction time is 3-5 minutes.

[0015] Preferably, the relationship between the concentration of the hydrogen peroxide solution and the soaking time in step 3 is:

[0016]

[0017] Where β is the immersion time (minutes), γ is the concentration of the hydrogen peroxide solution (%), and a and b are constants.

[0018] Preferably, in the step 4, the black spike teff seeds are soaked in 10-30 degrees Celsius water for 10-20 hours, the water is changed once every 3 hours during the soaking period, the black spike teff seeds after soaking are scrubbed and washed, and the water is changed repeatedly for a total of 5-6 times, and then the seeds are placed in a moist and cool place for 5-7 days.

[0019] Preferably, the concentration of the gibberellin solution, the soaking time and the soaking temperature in step 5 are related to each other.

[0020] Department:

[0021]

[0022] Wherein, G is the concentration of the gibberellin solution (mg / L), k and b are constants, T is the soaking temperature (degrees Celsius), and t is the soaking time (hours).

[0023] Preferably, in step seven, the culturing time of the Eragrostis nigra seeds in the light incubator is 10-20 hours, and the culturing temperature is 13-28 degrees Celsius.

[0024] Preferably, the preset culture medium in step 6 specifically consists of the following components:

[0025] 10-20 parts by mass of potassium nitrate, 1-2 parts by mass of potassium dihydrogen phosphate, 2-5 parts by mass of calcium chloride, 2-5 parts by mass of magnesium sulfate, 0.1-0.3 parts by mass of iron salt, 0.005-0.01 parts by mass of boron, 500-600 parts by mass of sucrose, 0.01-0.03 parts by mass of glycine, and 0.01-0.03 parts by mass of plant hormones.

[0026] Preferably, the culture medium specifically consists of the following components:

[0027] 10 parts by mass of potassium nitrate, 1 part by mass of potassium dihydrogen phosphate, 2 parts by mass of calcium chloride, 2 parts by mass of magnesium sulfate, 0.1 parts by mass of iron salt, 0.005 parts by mass of boron, 500 parts by mass of sucrose, 0.01 parts by mass of glycine, and 0.01 parts by mass of plant hormone. The culture medium can provide a suitable germination environment for seeds and improve the germination rate of seeds. At the same time, the seedlings after germination can grow stronger and have stronger stress resistance under sufficient nutrition supply, energy support and growth regulation, laying a good foundation for subsequent growth and development.

[0028] Preferably, the iron salt is a chelate of ferrous sulfate and disodium ethylenediaminetetraacetate.

[0029] Preferably, the plant hormone is any one of indoleacetic acid and benzylaminopurine.

[0030] The present invention provides a treatment method for improving the germination rate of black stigma grass seeds. It has the following beneficial effects:

[0031] 1. The method for improving the germination rate of black stigma teff seeds comprises the following steps: drying the collected black stigma teff seeds with a blower in step 2, thereby reducing the moisture content of the seeds. This operation can reduce the risk of seeds getting moldy and rotting during storage, extend the shelf life of the seeds, and thus ensure that the seeds are in a better health state during subsequent processing and cultivation; in step 3, the seeds are soaked in a hydrogen peroxide solution, and since hydrogen peroxide has strong oxidizing properties, it can kill the pathogens carried by the seeds. This reduces the probability of seeds being infected by diseases during germination and increases the possibility of healthy germination of seeds.

[0032] Second, the treatment method for improving the germination rate of black stigma teff seeds, by soaking the seeds in hydrogen peroxide in step three, for seeds with a certain hardness or poor permeability of the seed coat, its oxidation can soften the seed coat, increase the permeability of the seed coat, which is conducive to the seeds absorbing water and oxygen, thereby promoting seed germination; the preset culture medium in step six contains potassium nitrate, potassium dihydrogen phosphate, calcium chloride, magnesium sulfate, iron salt, boron, sucrose, glycine and plant hormones. These ingredients can provide a suitable germination environment for seeds, improve the germination rate of seeds, and the seedlings after germination grow stronger and stronger under sufficient nutrition supply, energy support and growth regulation, and have stronger stress resistance, laying a good foundation for subsequent growth and development.

[0033] 3. The method for improving the germination rate of black stigma seeds, after removing the seed hairs in step 2, the seeds are more likely to contact with the soil during sowing. Removal of the seed hairs reduces the hindrance of the seed hairs to the contact between the seeds and the soil, which is conducive to the seeds to better absorb water and nutrients in the soil, thereby improving the growth efficiency of the seeds after germination.

[0034] Fourth, the treatment method for improving the germination rate of black stigma grass seeds is to place the seeds treated with gibberellin solution in a light incubator for cultivation, and light can provide energy for seed germination and promote seed germination. Moreover, in the light incubator, environmental factors such as temperature and light can be precisely controlled, and such a stable environment helps the seedlings to better adapt to the environment and enhance the seedlings' ability to adapt to subsequent possible environmental changes. DETAILED DESCRIPTION

[0035] The present invention will be further described in detail below in conjunction with specific embodiments. The embodiments of the present invention are provided for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

[0036] In the first embodiment, the present invention provides a technical solution: a method for improving the germination rate of black stigma seeds, comprising the following steps:

[0037] Step 1: Collect black teff seeds;

[0038] Step 2, removing the receptacle and seed hairs, using a blower to dry the collected black stigma seeds, the moisture content of the dried seeds is reduced, the risk of mildew and rot during storage can be reduced, and the shelf life of the seeds can be extended, and the air-dried seeds are then sieved; sieving can achieve preliminary separation of the receptacle and the seeds; removing impurities and the receptacle, and finally rubbing the seed hairs with a friction machine, the friction wheel of the friction machine rotates 100 times / min, the friction pressure of the friction wheel may be 0.1 kg / cm2, and the friction time is 3 minutes;

[0039] After the seed hairs are removed, the seeds are more likely to come into contact with the soil when sowing, which is beneficial for absorbing moisture and nutrients in the soil.

[0040] Step 3: Seed treatment: soaking the seeds in hydrogen peroxide solution;

[0041] The relationship between the concentration of hydrogen peroxide solution and the immersion time is:

[0042]

[0043] Where β is the immersion time (minutes), γ is the concentration of hydrogen peroxide solution (%), and a and b are constants

[0044] Soaking in hydrogen peroxide can kill these pathogens and reduce the chance of seeds being infected by diseases during the germination process; for some seeds with a certain hardness or poor permeability in the seed coat, the oxidizing effect of hydrogen peroxide can soften the seed coat and increase its permeability.

[0045] Step 4: Rinse the seeds soaked in hydrogen peroxide solution with water, wherein the grass seeds are soaked in 30 degrees Celsius water for 10 hours, and the water is changed once every 3 hours during the soaking period. After soaking, the black spike teff seeds are scrubbed and washed, and the water is changed repeatedly for a total of 5 times, and then the seeds are placed in a moist and cool place for 5 days;

[0046] Step 5: Soak the rinsed seeds in a gibberellin solution to obtain seeds treated with gibberellin; in step 5, the relationship between the concentration of the gibberellin solution, the soaking time and the soaking temperature is:

[0047]

[0048] Wherein, G is the concentration of gibberellin solution (mg / L), k and b are constants, T is the temperature during immersion (degrees Celsius), and t is the immersion time (hours);

[0049] Gibberellic acid promotes the growth of stems and leaves of seedlings. After the seeds soaked in gibberellin solution germinate, the elongation of the internodes of the seedlings accelerates.

[0050] Step 6: Place the seeds treated with the gibberellin solution in a culture dish containing a preset culture medium; wherein the culture medium specifically comprises the following components:

[0051] 10 parts by mass of potassium nitrate, 1 part by mass of potassium dihydrogen phosphate, 2 parts by mass of calcium chloride, 2 parts by mass of magnesium sulfate, 0.1 parts by mass of iron salt, 0.0051 parts by mass of boron, 500 parts by mass of sucrose, 0.01 parts by mass of glycine, and 0.01 parts by mass of plant hormones;

[0052] The iron salt is specifically a chelate of ferrous sulfate and disodium ethylenediaminetetraacetate;

[0053] The plant hormone is specifically indoleacetic acid;

[0054] Step 7: Place the seeds treated with the gibberellin solution in a light incubator for cultivation to obtain the finally cultivated black stigma seeds. Light can provide energy for seed germination and promote seed germination. The black stigma seeds are placed in a light incubator for cultivation for 10 hours at a temperature of 13 degrees Celsius.

[0055] The second embodiment is based on the first embodiment.

[0056] The following steps are involved:

[0057] Step 1: Collect black teff seeds;

[0058] Step 2, removing the receptacle and seed hairs, using a blower to dry the collected black stigma seeds, the moisture content of the dried seeds is reduced, the risk of mildew and rot during storage can be reduced, and the shelf life of the seeds can be extended, and the air-dried seeds are then sieved; sieving can achieve preliminary separation of the receptacle and the seeds; removing impurities and the receptacle, and finally rubbing the seed hairs with a friction machine, the friction wheel of the friction machine rotates 300 times / min, the friction pressure of the friction wheel may be 0.3 kg / cm2, and the friction time is 5 minutes;

[0059] After the seed hairs are removed, the seeds are more likely to come into contact with the soil when sowing, which is beneficial for absorbing moisture and nutrients in the soil.

[0060] Step 3: Seed treatment: soaking the seeds in hydrogen peroxide solution;

[0061] The relationship between the concentration of hydrogen peroxide solution and the immersion time is:

[0062]

[0063] Where β is the immersion time (minutes), γ is the concentration of hydrogen peroxide solution (%), and a and b are constants

[0064] Soaking in hydrogen peroxide can kill these pathogens and reduce the chance of seeds being infected by diseases during the germination process; for some seeds with a certain hardness or poor permeability in the seed coat, the oxidizing effect of hydrogen peroxide can soften the seed coat and increase its permeability.

[0065] Step 4: Rinse the seeds soaked in hydrogen peroxide solution with water, wherein the grass seeds are soaked in 30 degrees Celsius water for 20 hours, and the water is changed once every 3 hours during the soaking period. After soaking, the black spike teff seeds are scrubbed and washed, and the water is changed repeatedly for a total of 6 times, and then the seeds are placed in a moist and cool place for 7 days;

[0066] Step 5: Soak the rinsed seeds in a gibberellin solution to obtain seeds treated with gibberellin; in step 5, the relationship between the concentration of the gibberellin solution, the soaking time and the soaking temperature is:

[0067]

[0068] Wherein, G is the concentration of gibberellin solution (mg / L), k and b are constants, T is the temperature during immersion (degrees Celsius), and t is the immersion time (hours);

[0069] Gibberellic acid promotes the growth of stems and leaves of seedlings. After the seeds soaked in gibberellin solution germinate, the elongation of the internodes of the seedlings accelerates.

[0070] Step 6: Place the seeds treated with the gibberellin solution in a culture dish containing a preset culture medium; wherein the culture medium specifically comprises the following components:

[0071] 20 parts by mass of potassium nitrate, 2 parts by mass of potassium dihydrogen phosphate, 5 parts by mass of calcium chloride, 5 parts by mass of magnesium sulfate, 0.3 parts by mass of iron salt, 0.01 parts by mass of boron, 600 parts by mass of sucrose, 0.03 parts by mass of glycine, and 0.03 parts by mass of plant hormones;

[0072] The iron salt is specifically a chelate of ferrous sulfate and disodium ethylenediaminetetraacetate;

[0073] The plant hormone is specifically benzylaminopurine;

[0074] Step 7: Place the seeds treated with the gibberellin solution in a light incubator for cultivation to obtain the finally cultivated black stigma seeds. Light can provide energy for seed germination and promote seed germination. The black stigma seeds are placed in a light incubator for cultivation for 20 hours at a temperature of 28 degrees Celsius.

[0075] The third embodiment, based on the first and second embodiments, includes the following steps:

[0076] Step 1: Collect black teff seeds;

[0077] Step 2, removing the receptacle and seed hairs, using a blower to dry the collected black stigma seeds, the moisture content of the dried seeds is reduced, the risk of mildew and rot during storage can be reduced, and the shelf life of the seeds can be extended, and the air-dried seeds are then sieved; sieving can achieve preliminary separation of the receptacle and the seeds; removing impurities and the receptacle, and finally rubbing the seed hairs with a friction machine, the friction wheel of the friction machine rotates 100 times / min, the friction pressure of the friction wheel may be 0.3 kg / cm2, and the friction time is 3 minutes;

[0078] After the seed hairs are removed, the seeds are more likely to come into contact with the soil when sowing, which is beneficial for absorbing moisture and nutrients in the soil.

[0079] Step 3: Seed treatment: soaking the seeds in hydrogen peroxide solution;

[0080] The relationship between the concentration of hydrogen peroxide solution and the immersion time is:

[0081]

[0082] Where β is the immersion time (minutes), γ is the concentration of hydrogen peroxide solution (%), and a and b are constants

[0083] Soaking in hydrogen peroxide can kill these pathogens and reduce the chance of seeds being infected by diseases during the germination process; for some seeds with a certain hardness or poor permeability in the seed coat, the oxidizing effect of hydrogen peroxide can soften the seed coat and increase its permeability.

[0084] Step 4: Rinse the seeds soaked in hydrogen peroxide solution with water, wherein the grass seeds are soaked in 10 degrees Celsius water for 10 hours, and the water is changed once every 3 hours during the soaking period. After soaking, the black stigma seeds are scrubbed and washed, and the water is changed repeatedly for a total of 6 times, and then the seeds are placed in a moist and cool place for 5 days;

[0085] Step 5: Soak the rinsed seeds in a gibberellin solution to obtain seeds treated with gibberellin; in step 5, the relationship between the concentration of the gibberellin solution, the soaking time and the soaking temperature is:

[0086]

[0087] Wherein, G is the concentration of gibberellin solution (mg / L), k and b are constants, T is the temperature during immersion (degrees Celsius), and t is the immersion time (hours);

[0088] Gibberellic acid promotes the growth of stems and leaves of seedlings. After the seeds soaked in gibberellin solution germinate, the elongation of the internodes of the seedlings accelerates.

[0089] Step 6: Place the seeds treated with the gibberellin solution in a culture dish containing a preset culture medium; wherein the culture medium specifically comprises the following components:

[0090] 10 parts by mass of potassium nitrate, 2 parts by mass of potassium dihydrogen phosphate, 2 parts by mass of calcium chloride, 5 parts by mass of magnesium sulfate, 0.3 parts by mass of iron salt, 0.005 parts by mass of boron, 500 parts by mass of sucrose, 0.03 parts by mass of glycine, and 0.03 parts by mass of plant hormones;

[0091] The iron salt is specifically a chelate of ferrous sulfate and disodium ethylenediaminetetraacetate;

[0092] The plant hormone is specifically benzylaminopurine;

[0093] Step 7: Place the seeds treated with the gibberellin solution in a light incubator for cultivation to obtain the finally cultivated black stigma seeds. Light can provide energy for seed germination and promote seed germination. The black stigma seeds are placed in a light incubator for cultivation for 20 hours at a temperature of 13 degrees Celsius.

[0094] The fourth embodiment, based on the first, second and third embodiments, includes the following steps:

[0095] Step 1: Collect black teff seeds;

[0096] Step 2, removing the receptacle and seed hairs, using a blower to dry the collected black stigma seeds, the moisture content of the dried seeds is reduced, the risk of mildew and rot during storage can be reduced, and the shelf life of the seeds can be extended, and the air-dried seeds are then sieved; sieving can achieve preliminary separation of the receptacle and the seeds; removing impurities and the receptacle, and finally rubbing the seed hairs with a friction machine, the friction wheel of the friction machine is 200 revolutions / minute, the friction pressure of the friction wheel may be 0.2 kg / cm2, and the friction time is 4 minutes;

[0097] After the seed hairs are removed, the seeds are more likely to come into contact with the soil when sowing, which is beneficial for absorbing moisture and nutrients in the soil.

[0098] Step 3: Seed treatment: soaking the seeds in hydrogen peroxide solution;

[0099] The relationship between the concentration of hydrogen peroxide solution and the immersion time is:

[0100]

[0101] Where β is the immersion time (minutes), γ is the concentration of hydrogen peroxide solution (%), and a and b are constants

[0102] Soaking in hydrogen peroxide can kill these pathogens and reduce the chance of seeds being infected by diseases during the germination process; for some seeds with a certain hardness or poor permeability in the seed coat, the oxidizing effect of hydrogen peroxide can soften the seed coat and increase its permeability.

[0103] Step 4: Rinse the seeds soaked in hydrogen peroxide solution with water, wherein the grass seeds are soaked in 15 degrees Celsius water for 15 hours, and the water is changed once every 3 hours during the soaking period. After soaking, the black stigma seeds are scrubbed and washed, and the water is changed repeatedly for a total of 5 times, and then the seeds are placed in a moist and cool place for 6 days;

[0104] Step 5: Soak the rinsed seeds in a gibberellin solution to obtain seeds treated with gibberellin; in step 5, the relationship between the concentration of the gibberellin solution, the soaking time and the soaking temperature is:

[0105]

[0106] Wherein, G is the concentration of gibberellin solution (mg / L), k and b are constants, T is the temperature during immersion (degrees Celsius), and t is the immersion time (hours);

[0107] Gibberellic acid promotes the growth of stems and leaves of seedlings. After the seeds soaked in gibberellin solution germinate, the elongation of the internodes of the seedlings accelerates.

[0108] Step 6: Place the seeds treated with the gibberellin solution in a culture dish containing a preset culture medium; wherein the culture medium specifically comprises the following components:

[0109] 15 parts by mass of potassium nitrate, 2 parts by mass of potassium dihydrogen phosphate, 5 parts by mass of calcium chloride, 4 parts by mass of magnesium sulfate, 0.2 parts by mass of iron salt, 0.01 parts by mass of boron, 600 parts by mass of sucrose, 0.03 parts by mass of glycine, and 0.01 parts by mass of plant hormones;

[0110] The iron salt is specifically a chelate of ferrous sulfate and disodium ethylenediaminetetraacetate;

[0111] The plant hormone is specifically indoleacetic acid;

[0112] Step 7: Place the seeds treated with the gibberellin solution in a light incubator for cultivation to obtain the finally cultivated black stigma seeds. Light can provide energy for seed germination and promote seed germination. The black stigma seeds are placed in a light incubator for cultivation for 20 hours at a temperature of 13 degrees Celsius.

[0113] The fourth embodiment, based on the first, second, third and fourth embodiments, comprises the following steps: step 1, collecting seeds of teff;

[0114] Step 2, removing the receptacle and seed hairs, using a blower to dry the collected black stigma seeds, the moisture content of the dried seeds is reduced, the risk of mildew and rot during storage can be reduced, and the shelf life of the seeds can be extended, and the air-dried seeds are then sieved; sieving can achieve preliminary separation of the receptacle and the seeds; removing impurities and the receptacle, and finally rubbing the seed hairs with a friction machine, the friction wheel of the friction machine rotates 300 times / min, the friction pressure of the friction wheel may be 0.3 kg / cm2, and the friction time is 5 minutes;

[0115] After the seed hairs are removed, the seeds are more likely to come into contact with the soil when sowing, which is beneficial for absorbing moisture and nutrients in the soil.

[0116] Step 3: Seed treatment: soaking the seeds in hydrogen peroxide solution;

[0117] The relationship between the concentration of hydrogen peroxide solution and the immersion time is:

[0118]

[0119] Where β is the immersion time (minutes), γ is the concentration of hydrogen peroxide solution (%), and a and b are constants

[0120] Soaking in hydrogen peroxide can kill these pathogens and reduce the chance of seeds being infected by diseases during the germination process; for some seeds with a certain hardness or poor permeability in the seed coat, the oxidizing effect of hydrogen peroxide can soften the seed coat and increase its permeability.

[0121] Step 4: Rinse the seeds soaked in hydrogen peroxide solution with water, wherein the grass seeds are soaked in 26.5 degrees Celsius water for 10 hours, and the water is changed once every 3 hours during the soaking period. After soaking, the black spike teff seeds are scrubbed and washed, and the water is changed repeatedly for a total of 5 times, and then the seeds are placed in a moist and cool place for 5 days;

[0122] Step 5: Soak the rinsed seeds in a gibberellin solution to obtain seeds treated with gibberellin; in step 5, the relationship between the concentration of the gibberellin solution, the soaking time and the soaking temperature is:

[0123]

[0124] Wherein, G is the concentration of gibberellin solution (mg / L), k and b are constants, T is the temperature during immersion (degrees Celsius), and t is the immersion time (hours);

[0125] Gibberellic acid promotes the growth of stems and leaves of seedlings. After the seeds soaked in gibberellin solution germinate, the elongation of the internodes of the seedlings accelerates.

[0126] Step 6: Place the seeds treated with the gibberellin solution in a culture dish containing a preset culture medium; wherein the culture medium specifically comprises the following components:

[0127] 15 parts by mass of potassium nitrate, 1.65 parts by mass of potassium dihydrogen phosphate, 3 parts by mass of calcium chloride, 4 parts by mass of magnesium sulfate, 0.15 parts by mass of iron salt, 0.01 parts by mass of boron, 550 parts by mass of sucrose, 0.01 parts by mass of glycine, and 0.02 parts by mass of plant hormones;

[0128] The iron salt is specifically a chelate of ferrous sulfate and disodium ethylenediaminetetraacetate;

[0129] The plant hormone is specifically indoleacetic acid;

[0130] Step 7: Place the seeds treated with the gibberellin solution in a light incubator for cultivation to obtain the finally cultivated black stigma seeds. Light can provide energy for seed germination and promote seed germination. The black stigma seeds are placed in a light incubator for cultivation for 15 hours at a temperature of 11 degrees Celsius.

[0131] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention are implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. A method for improving the germination rate of teff seeds, characterized in that: The following steps are involved: Step 1: Collect black teff seeds; Step 2: removing the receptacle and seed hairs, drying the collected black spike teff seeds with a blower, sieving the air-dried seeds to remove impurities and the receptacle, and finally rubbing the seeds with a friction machine to remove the seed hairs; Step 3: Seed treatment: soaking the seeds in hydrogen peroxide solution; Step 4: Rinse the seeds soaked in the hydrogen peroxide solution with water; Step 5: soaking the rinsed seeds in a gibberellin solution to obtain seeds treated with gibberellin; Step 6: Place the seeds treated with the gibberellin solution in a culture dish containing a preset culture medium; Step seven: placing the seeds treated with the gibberellin solution in a light incubator for cultivation to obtain the finally cultivated black stigma seeds.

2. A method for improving the germination rate of teff seeds according to claim 1, characterized in that: The friction wheel of the friction machine in step 2 rotates at 100-300 revolutions per minute, the friction pressure of the friction wheel may be 0.1-0.3 kg / cm2, and the friction time is 3-5 minutes.

3. A method for improving the germination rate of teff seeds according to claim 1, characterized in that: The relationship between the concentration of the hydrogen peroxide solution and the soaking time in step 3 is: Where β is the immersion time (minutes), γ is the concentration of the hydrogen peroxide solution (%), and a and b are constants.

4. A method for improving the germination rate of teff seeds according to claim 1, characterized in that: In the step 4, the black spike teff seeds are soaked in 10-30 degrees Celsius water for 10-20 hours, and the water is changed once every 3 hours during the soaking period. The black spike teff seeds after soaking are scrubbed and washed, and the water is changed repeatedly for a total of 5-6 times. The seeds are then placed in a moist and cool place for 5-7 days.

5. A method for improving the germination rate of teff seeds according to claim 3, characterized in that: The relationship between the concentration of the gibberellin solution, the soaking time and the soaking temperature in step 5 is: Wherein, G is the concentration of the gibberellin solution (mg / L), k and b are constants, T is the soaking temperature (degrees Celsius), and t is the soaking time (hours).

6. A method for improving the germination rate of Erigeron nigrostach seeds according to claim 1, characterized in that: In the step 7, the black stigma seeds are placed in a light incubator for culturing for 10-20 hours at a temperature of 13-28 degrees Celsius.

7. A method for improving the germination rate of Erigeron nigrostach seeds according to claim 1, characterized in that: The preset culture medium in step 6 specifically consists of the following components: 10-20 parts by mass of potassium nitrate, 1-2 parts by mass of potassium dihydrogen phosphate, 2-5 parts by mass of calcium chloride, 2-5 parts by mass of magnesium sulfate, 0.1-0.3 parts by mass of iron salt, 0.005-0.01 parts by mass of boron, 500-600 parts by mass of sucrose, 0.01-0.03 parts by mass of glycine, and 0.01-0.03 parts by mass of plant hormones.

8. A method for improving the germination rate of Erigeron nigrostach seeds according to claim 7, characterized in that: The culture medium specifically consists of the following components: 10 parts by mass of potassium nitrate, 1 part by mass of potassium dihydrogen phosphate, 2 parts by mass of calcium chloride, 2 parts by mass of magnesium sulfate, 0.1 parts by mass of iron salt, 0.005 parts by mass of boron, 500 parts by mass of sucrose, 0.01 parts by mass of glycine, and 0.01 parts by mass of plant hormones.

9. A method for improving the germination rate of teff seeds according to claim 8, characterized in that: The iron salt is specifically a chelate of ferrous sulfate and disodium ethylenediaminetetraacetate.

10. A method for improving the germination rate of Erigeron nigrostachiana seeds according to claim 1, characterized in that: The plant hormone is specifically any one of indoleacetic acid and benzylaminopurine.

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