A miticidal composition and use thereof

Abstract

The application belongs to the technical field of pesticides, and relates to a kind of acaricidal composition and its application, the acaricidal composition contains active ingredient A and active ingredient B;Active ingredient A is a compound of formula (I), and active ingredient B is any one of lufenuron and fluazaindolam.The acaricidal composition can efficiently control phytophagous mites, can obviously reduce the mite population, improve the rapid availability and delay the development of drug resistance.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticides, and particularly relates to a kind of acaricidal composition and its application. BACKGROUND

[0002] Tetranychus urticae Koch belongs to Tetranychidae and Tetranychus, is an important omnivorous spider mite, host plants are widely, more than 1100 species in 140 families have been found, from mid-June to mid-August is the period of Tetranychus urticae Koch rampant, often with the larva, nymph and adult mites sucking and damaging the leaves of vegetables, flowers and fruit trees, causing leaf chlorosis and scorching and falling off, seriously affecting photosynthesis, inhibiting the growth of vegetables and flowers and fruit development, ultimately affecting crop yield and quality. In recent years, Tetranychus urticae Koch has changed from a secondary spider mite to a major one, and has become one of the important spider mites of vegetables, flowers and fruit trees.

[0003] Lufenuron belongs to chitin synthesis inhibitors, the pesticide kills pests by acting on insect larvae and preventing the molting process, has stomach poison and contact killing, multiple ovicidal, high-efficiency insecticidal effects, and has little effect on natural enemies, and is very safe to humans and animals.

[0004] Fluazinam belongs to dinitroaniline fungicides, is a broad-spectrum and high-efficiency protective fungicide, is highly effective on Alternaria, Imperata, Plasmopara, Venturia and other fungi, and also has the effect of controlling phytophagous mites.

[0005] Due to the unreasonable use of pesticides and the characteristics of fast reproduction and serious overlapping of generations of Tetranychus urticae Koch, Tetranychus urticae Koch has developed serious resistance to lufenuron, fluazinam and other insecticides. Therefore, in order to slow down the resistance of Tetranychus urticae Koch to lufenuron and fluazinam and improve the control effect, the present application compounds formula (I) with lufenuron and fluazinam, which can obviously increase the effect within a certain mixing range, reduce the cost, reduce the amount of pesticides, prolong the effective period and delay the development of resistance. SUMMARY

[0006] In order to solve the above problems in the prior art, the present application provides an acaricidal composition, which can effectively control phytophagous spider mites, has a synergistic effect within a certain proportion range, reduces the use cost, reduces the amount of pesticides, prolongs the effective period and delays the development of resistance.

[0007] In order to solve the above technical problems, the present application provides the following technical scheme: an acaricidal composition, which comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula (I):

[0008] The active ingredient B is any one of lufenuron and fluazinam;

[0009] Further, the mass ratio of the active ingredient A and the active ingredient B is 1:30-30:1.

[0010] Further, the mass ratio of the compound of formula (I) and the compound of formula (II) is 1:24-24:1.

[0011] Further, the mass ratio of the compound of formula (I) and the compound of formula (II) is 1:24, 1:18, 1:14, 1:7, 5:2, 7:1, 15:1, 20:1, 24:1.

[0012] Further, the mass ratio of the compound of formula (I) and fluazinam is 1:20-24:1.

[0013] Further, the mass ratio of the compound of formula (I) and fluazinam is 1:20, 1:15, 1:10, 1:5, 3:2, 5:1, 12:1, 24:1.

[0014] Further, the mass ratio of the compound of formula (I) and the compound of formula (II) is 1:24-7:1.

[0015] Further, the mass ratio of the compound of formula (I) and fluazinam is 1:15-5:1.

[0016] Further, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 1-95wt% based on 100wt% of the total weight of the acaricidal composition.

[0017] Further, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 5-80wt%.

[0018] Further, the acaricidal composition further comprises an agriculturally acceptable auxiliary ingredient in addition to the active ingredients, and the auxiliary ingredient is selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

[0019] The wetting agent is selected from one or more of alkyl benzene sulfonate, alkyl naphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl phthalate sulfonate sodium, alpha olefin sulfonate, alkyl phenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkyl phenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, soap nut powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0020] The dispersant is selected from one or more of lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkyl phenol polyoxyethylene, alkyl phenol polyoxyethylene methylether condensate sulfate, fatty amine polyoxyethylene, glycerol fatty acid ester polyoxyethylene, polycarboxylate, polyacrylate, phosphate, EO-PO block copolymer, and EO-PO graft copolymer; and / or

[0021] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkyl phenol formaldehyde resin polyoxyethylene, phenylethyl phenol polyoxyethylene polypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styryl phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkyl phenol ether phosphate; and / or

[0022] The thickening agent is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and white carbon; and / or

[0023] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0024] The antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0025] The antifoaming agent is selected from one or more of C 10 -C 20 saturated fatty acid compound, silicone oil, silicone compound, C8-C 10 fatty alcohol; and / or

[0026] The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oil, vegetable oil derivative, and water; and / or

[0027] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, capsol, and 1,2-benzisothiazolin-3-one; and / or

[0028] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon, talc, montmorillonite, and starch; and / or

[0029] The synergist is selected from the group consisting of phosphorodithioic acid, piperonyl butoxide; and / or

[0030] The carrier is selected from the group consisting of one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0031] Further, the acaricidal composition can be prepared into an agriculturally acceptable formulation form selected from the group consisting of solid formulations and / or liquid formulations;

[0032] The solid formulation includes a powder, a granule, a pellet, a tablet, a bar, a wettable powder, an oil dispersible powder, a cream powder, a water dispersible granule, a cream granule, a water dispersible tablet, a soluble powder, a soluble tablet or a soluble granule;

[0033] The liquid formulation includes a soluble liquid, a soluble gel, an oil, a spreadable oil, an emulsion oil, an emulsion, a dispersible liquid, a paste, an aqueous emulsion, an oil emulsion, a microemulsion, a lipid, a suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspoemulsion, a microcapsule suspension-aqueous emulsion or a microcapsule suspension-suspoemulsion;

[0034] Further, the powder is a free-flowing powder formulation containing an active ingredient suitable for dusting or spreading;

[0035] Further, the granule is a granular formulation containing an active ingredient which is free-flowing within a certain particle size range;

[0036] Further, the pellet is a spherical formulation (generally greater than 6 mm in diameter) containing an active ingredient;

[0037] Further, the tablet is a tabular formulation having a certain shape and size containing an active ingredient (generally having two flat faces or a convex face with a distance between the two faces less than the diameter);

[0038] Further, the bar is a strip or rod-shaped formulation containing an active ingredient (generally several centimeters in length and a few millimeters in width / diameter, i.e., the length is greater than the diameter / width);

[0039] Further, the wettable powder is a powder formulation in which an active ingredient is dispersed as a suspension in water;

[0040] Further, the oil dispersible powder is a powder formulation in which an active ingredient is dispersed as a suspension in an organic solvent;

[0041] Further, the cream powder is a powder formulation in which an active ingredient is dissolved in an organic solvent and coated in a soluble or insoluble inert ingredient, and dispersed in water to form an oil-in-water emulsion;

[0042] Further, the water dispersible granules are granular formulations which disintegrate in water and disperse the active ingredient into a suspension;

[0043] Further, the emulsifiable granules are granular formulations in which the active ingredient is dissolved in an organic solvent and is coated with a soluble or insoluble inert ingredient to form an oil-in-water emulsion when dispersed in water;

[0044] Further, the water dispersible tablets are tablet formulations which disintegrate in water and disperse the active ingredient into a suspension;

[0045] Further, the soluble powders are powdery formulations in which the active ingredient forms a true solution in water, and can contain an inert ingredient which is insoluble in water;

[0046] Further, the soluble granules are granular formulations in which the active ingredient forms a true solution in water, and can contain an inert ingredient which is insoluble in water;

[0047] Further, the soluble tablets are tablet formulations in which the active ingredient forms a true solution in water, and can contain an inert ingredient which is insoluble in water;

[0048] Further, the soluble solutions are liquid formulations which form a clear or translucent liquid containing the active ingredient when diluted with water, and can contain an inert ingredient which is insoluble in water;

[0049] Further, the soluble gels are gelatinous formulations which form a true solution containing the active ingredient when diluted with water;

[0050] Further, the oil formulations are liquid formulations which form a homogeneous phase containing the active ingredient when diluted (or not diluted) with an organic solvent;

[0051] Further, the spreadable oil formulations are oil formulations which automatically spread on the surface of water to form an oil film containing the active ingredient;

[0052] Further, the emulsifiable concentrates are homogeneous liquid formulations which disperse into an emulsion containing the active ingredient when diluted with water;

[0053] Further, the emulsions are emulsion formulations which disperse into an emulsion containing the active ingredient when diluted with water;

[0054] Further, the dispersible concentrates are homogeneous liquid formulations which disperse into a suspension containing the active ingredient when diluted with water;

[0055] Further, the pastes are water-based paste formulations which form a film containing the active ingredient, and are generally used directly;

[0056] Further, the aqueous emulsions are emulsion formulations in which the active ingredient (or an organic solution thereof) forms an emulsion in water;

[0057] Further, the oil emulsion is a liquid preparation of the active ingredient (or its aqueous solution) in oil forming an emulsion;

[0058] Further, the microemulsion is a liquid preparation of the active ingredient in water forming a transparent or translucent microemulsion, which is used directly or after dilution with water;

[0059] Further, the lipid preparation is an oil or fat-based viscous preparation containing the active ingredient, which is generally used directly;

[0060] Further, the suspension is a stable liquid preparation of the active ingredient in the form of solid particles dispersed in water, which is generally used after dilution with water;

[0061] Further, the microcapsule suspension is a stable liquid preparation of the active ingredient in the form of microcapsules dispersed in a liquid;

[0062] Further, the oil suspension is a stable liquid preparation of the active ingredient in the form of solid particles dispersed in a liquid, which is generally used after dilution with an organic solvent;

[0063] Further, the dispersible oil suspension is a stable liquid preparation of the active ingredient in the form of solid particles dispersed in a non-aqueous medium, which is generally used after dilution with water;

[0064] Further, the suspoemulsion is a heterogeneous liquid preparation of the active ingredient in the form of solid particles and water-insoluble microdroplets stably dispersed in a continuous aqueous phase;

[0065] Further, the microcapsule suspension-suspension is a stable liquid preparation of the active ingredient in the form of microcapsules and solid particles dispersed in water;

[0066] Further, the microcapsule suspension-water emulsion is a heterogeneous liquid preparation of the active ingredient in the form of microcapsules and microdroplets stably dispersed in a continuous aqueous phase;

[0067] Further, the microcapsule suspension-suspoemulsion is a heterogeneous liquid preparation of the active ingredient in the form of microcapsules, solid particles, and microdroplets stably dispersed in a continuous aqueous phase;

[0068] Further, the solid preparation is selected from the group consisting of wettable powder, water dispersible granule; and the liquid preparation is selected from the group consisting of soluble liquid, emulsifiable concentrate, aqueous emulsion, suspension, suspoemulsion, microemulsion, dispersible oil suspension;

[0069] Further, the solid preparation is selected from the group consisting of wettable powder, water dispersible granule; and the liquid preparation is selected from the group consisting of emulsifiable concentrate, aqueous emulsion, suspension;

[0070] Further, the preparation method of the wettable powder is that the effective component and other auxiliary agents are pre-pulverized and uniformly mixed, and then are pulverized by an air flow pulverizer to a fineness meeting the requirement that at least 98wt% passes through a 45mu m test sieve, so as to obtain the wettable powder product;

[0071] Further, the preparation method of the water dispersible granule is that the effective component is added into the carrier according to the proportion of the example formula, and the surfactant and other functional auxiliary agents are added and mixed, and then 10-25% of water is added after air flow pulverization, and then the kneading, granulation, drying and screening are performed to obtain the water dispersible granule product;

[0072] Further, the preparation method of the emulsifiable concentrate is that the effective component is added into the carrier according to the proportion of the example formula, and the surfactant and other functional auxiliary agents are added and uniformly mixed in the stirring mixing kettle, so as to obtain the emulsifiable concentrate product;

[0073] Further, the preparation method of the emulsion in water is that the effective component is dissolved into the solvent according to the proportion of the example formula, and the emulsifier is added, so that the dissolution is uniform oil phase; the deionized water and the antifreezing agent are mixed together to form uniform water phase; the water phase is added into the oil phase, and the defoaming agent is added after uniform stirring, and then the high-speed shearing is performed to form the emulsion in water product with good dispersion;

[0074] Further, the preparation method of the suspension concentrate is that the effective component, the surfactant and other functional auxiliary agents are sequentially placed in a reaction kettle according to the proportion of the formula, and then water is added and uniformly mixed, and then the high-speed shearing and wet sand grinding are performed, and finally the uniform filtration is performed to obtain the suspension concentrate product;

[0075] The application further discloses application of the pesticide composition to the mite control.

[0076] Further, the mite is a phytophagous mite.

[0077] Further, the phytophagous mite is Panonychus citri McGregor, Eotetranychus kankitus (Ehara), Phyllocoptes oleivora, Tetranychus cinnabarinus (Boisduval) and Tetranychus urticae Koch.

[0078] Further, the pesticide composition and / or the preparation thereof is applied to the mite in need of control or the medium in which the mite grows in an effective dose.

[0079] The application has the following beneficial effects:

[0080] (1) The acaricidal composition of the present application has obvious synergistic effect at a certain ratio, especially has good control effect on Tetranychus urticae Koch;

[0081] (2) Can obviously reduce phytophagous mite population, improve available nature and delay development of drug resistance;

[0082] (3) Safe and efficient, safe to crops, non-target organisms, beneficial organisms and natural enemies, has the effect of increasing yield and ensuring harvest. DETAILED DESCRIPTION

[0083] In order to make the technical scheme, purpose and advantages of the present application clearer and more apparent, the present application is described by the following specific examples, but the present application can be realized in various forms and should not be limited by the embodiments described herein.

[0084] Preparation example of preparation:

[0085] Preparation example 1: 14% compound of formula (I) · bifenurate suspension (10:4)

[0086] Preparation formula: 10% compound of formula (I), 4% bifenurate, 2% calcium dodecylbenzenesulfonate, 3% fatty amine polyoxyethylene ether, 2% polycarboxylate, 5% sodium naphthalenesulfonate, 0.5% sodium p-hydroxybenzoate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 4% ethylene glycol, 0.25% dimethyl silicone oil, and deionized water to make up the balance;

[0087] Preparation method: according to the preparation formula ratio, the effective ingredients, surfactants and other functional additives are placed in the reaction kettle in turn, mixed with deionized water, sheared at high speed, wet sand ground, and finally filtered to obtain the suspension product.

[0088] Preparation example 2: 20% compound of formula (I) · fluazinam suspension (12:8)

[0089] Preparation formula: 12% compound of formula (I), 8% fluazinam, 4% calcium dodecylbenzenesulfonate, 1.5% polyether, 4% sodium lignosulfonate, 0.2% sodium sorbate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 4.5% propylene glycol, 0.25% dimethyl silicone oil, and deionized water to make up the balance;

[0090] Preparation method: same as preparation example 1.

[0091] Preparation example 3: 4% compound of formula (I) · bifenurate emulsion (2.5:1.5)

[0092] Preparation formula: 2.5% compound of formula (I), 1.5% bifenurate, 15% propylene carbonate, 4.5% sodium dodecyl sulfate, 12% fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance;

[0093] Preparation method: according to the formulation ratio of the example, the active ingredient is added into the solvent, and the surfactant and other functional adjuvants are added into the solvent, and then the mixture is stirred in the mixing kettle to obtain the emulsifiable concentrate product.

[0094] Preparation example 4: 7.5% compound of formula (I) + lufenuron emulsion in water (4.5:3)

[0095] Preparation formula: 4.5% compound of formula (I), 3% lufenuron, 5% xylene, 12% cyclohexanone, 4% sodium dodecylbenzenesulfonate, 6.5% ethylene oxide-propylene oxide copolymer, 4% ethylene glycol, 0.5% glycerol, 0.2% silicone antifoaming agent, 0.1% xanthan gum, 0.2% benzoic acid, and deionized water to make up the balance;

[0096] Preparation method: according to the formulation ratio of the example, the active ingredient is dissolved into the solvent, and the emulsifier is added to form a uniform oil phase, and deionized water and antifreeze are mixed to form a uniform water phase; under high-speed shearing, the water phase is added to the oil phase to form a well-dispersed emulsion product.

[0097] Preparation example 5: 36% compound of formula (I) + fluazinam wettable powder (18:18)

[0098] Preparation formula: 18% compound of formula (I), 18% fluazinam, 15% sodium lignosulfonate, 6% castor oil polyoxyethylene ether phosphate, 5% naphthalenesulfonate, 6% bentonite, 4.5% white carbon black, and kaolin to make up the balance.

[0099] Preparation method: according to the formulation ratio of the example, the active ingredient is added into the carrier, and the surfactant and other functional adjuvants are added into the carrier, and then the mixture is mixed and air-jet pulverized to obtain the wettable powder product.

[0100] Preparation example 6: 40% compound of formula (I) + lufenuron wettable powder (16:24)

[0101] Preparation formula: 16% compound of formula (I), 24% fluazinam, 10% sodium lignosulfonate, 2% castor oil polyoxyethylene ether phosphate, 5% naphthalenesulfonate, 10% starch, 5% white carbon black, and kaolin to make up the balance.

[0102] Preparation method: same as preparation example 5.

[0103] Example 1 of indoor toxicity

[0104] The example refers to the guidelines for indoor biological testing of pesticides, part 7: determination of combined action of mixtures NY / T 1154.7-2006.

[0105] Test insect source: Two-spotted spider mites (reared in the research and development center) were selected for indoor rearing and consistent physiological state. Temperature (25 ± 1) °C. Relative humidity 65% ± 5%, photoperiod 16 / 8 h (L / D).

[0106] Formulation of the agent: The original drug was dissolved in acetone, and then diluted with 0.1% Tween-80 aqueous solution. Single-agent stock solutions were prepared, and each group of formulations was designed according to the purpose of mixing and the activity of the agent. Each single agent and each group of mixed agents was prepared in five series of concentrations by the method of equal proportion.

[0107] Test method for sublethal concentration of each treatment on adult mites: Select female adult mites with consistent growth and stick the back to a glass slide with double-sided tape. After 1 hour, examine under a microscope and select 20 individuals in good condition. Then immerse the slide in each concentration of the agent for 5 seconds, remove the excess liquid with filter paper, and place it in a light incubator at a temperature of (25 ± 1) °C and a relative humidity of 65% ± 5% with a photoperiod of 16 / 8 h (L / D). After 36 hours of agent treatment, count the number of dead mites.

[0108] Each treatment was repeated at least 4 times, and a treatment without the agent (containing all organic solvents and emulsifiers) was used as a blank control.

[0109] Data statistics and analysis: Record the total number of mites and the number of dead mites (death criteria: use a needle to move the mite's appendages, and if it does not move, it is considered dead; if it moves, it is considered alive).

[0110] Test method for sublethal effect of each treatment on adult mites: Take several fresh cowpea leaves and place them in a culture dish with a sponge. On each leaf, introduce 200 female adult mites that have just emerged within 24 hours. Let them rest for 30 minutes, then spray them with the LC 10 dose of each treatment.

[0111] Use a spray tower to spray quantitatively. Take 2 mL of the agent to be used, and spray for 60 seconds. After the liquid dries, continue to raise them in a light incubator.

[0112] After 24 hours, select healthy and robust adult mites and place them in fresh cowpea leaf dishes with a diameter of 2 cm and without the agent for single-head feeding. Observe at least 100 mites per treatment, and record the number of eggs laid by the adult mites every 24 hours until all the adult mites die. Use water as a control.

[0113] Data analysis:

[0114] According to the survey data, calculate the corrected mortality rate of each treatment. Calculate according to the following formula, and the results are rounded to two decimal places:

[0115]

[0116] Wherein:

[0117] P - mortality, in percentage (%);

[0118] K - represents the number of dead insects, in head;

[0119] N - represents the total number of insects, in head.

[0120]

[0121] Wherein:

[0122] P1 - corrected mortality, in percentage (%);

[0123] P t - treatment mortality, in percentage (%);

[0124] P0 - blank control mortality, in percentage (%).

[0125] If the control mortality is < 5%, no correction is needed; if the control mortality is between 5% and 20%, correction should be made; if the control mortality is > 20%, the test needs to be redone.

[0126] The data are processed by using the method of odds ratio analysis. The DPS statistical analysis system can be used to analyze the LC 10 values, LC 50 values and their 95% confidence limits, and b values of the toxicity regression line, so as to evaluate the activity of the test agent on the biological test material.

[0127] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0128]

[0129] Wherein:

[0130] ATI - measured toxicity index of the mixture;

[0131] S - LC 50 of the standard acaricide, in milligrams per liter (mg / L);

[0132] M - LC 50 of the mixture, in milligrams per liter (mg / L).

[0133] TTI = TI A *P A + TI B *P B

[0134] Wherein:

[0135] TTI - theoretical toxicity index of the mixture;

[0136] TI A Toxicity index of A agent;

[0137] P A Percentage of A agent in the mixture, in percentage (%);

[0138] TI B Toxicity index of B agent;

[0139] P B Percentage of B agent in the mixture, in percentage (%).

[0140]

[0141] In the formula:

[0142] CTC - Co-toxicity coefficient;

[0143] ATI - Actual toxicity index of the mixture;

[0144] TTI - Theoretical toxicity index of the mixture.

[0145] The co-toxicity coefficient CTC of the mixture is greater than or equal to 120, which shows synergistic effect; CTC is less than or equal to 80, which shows antagonistic effect; 80 < CTC < 120, which shows additive effect.

[0146] Test results:

[0147] The test results in Table 1 and Table 2 show that lufenuron and fluazinam have high toxicity to T. urticae, and the LC 50 The compound of formula (I) and lufenuron show synergistic effect in the range of 1:24 to 24:1; the compound of formula (I) and fluazinam show synergistic effect in the range of 1:20 to 24:1.

[0148] Table 1 Joint toxicity determination of the compound of formula (I) and lufenuron in different ratios to T. urticae

[0149]

[0150] Table 2 Joint toxicity determination of the compound of formula (I) and fluazinam in different ratios to T. urticae

[0151]

[0152]

[0153] The sub-lethal doses LC 10The effects of the treatment of T. urticae adult on its life span and egg hatching rate are shown in Table 3. It can be seen that the life span and egg production of the treated adult are reduced to different degrees, and the most obvious reduction is observed in the life span, egg production and egg hatching rate of the adult treated with the sub-lethal dose LC 10 The most obvious reduction in the life span, egg production and egg hatching rate of the adult treated with the sub-lethal dose LC 10 The most obvious reduction in the life span, egg production and egg hatching rate of the adult treated with the sub-lethal dose LC

[0154] Table 3 Effects of the treatment of T. urticae adult with the sub-lethal dose of the compound of formula (I) and lufenuron or flonicamid in different ratios on its life span, egg production and egg hatching rate

[0155]

[0156] Note: The above data are the average of the data of each individual T. urticae observed in each treatment and the integer is retained.

[0157] Field test Example 1 Field test for the control of T. urticae on greenhouse bean

[0158] Test crop: bean, variety: garden bean;

[0159] Test object: T. urticae;

[0160] Test site: a greenhouse in Pingdu, Qingdao, Shandong Province, with good management and uniform growth, and no use of any pesticide after emergence until before the pesticide application, and the pesticide application period is the initial flowering period, and T. urticae occurs heavily.

[0161] Tested agents:

[0162] Table 4 Test treatments and dosage

[0163] Tested treatment Tested agent Dilution ratio 1 14% compound of formula (I)·hydroprene SC (10:4) 4000 2 4% compound of formula (I)·hydroprene EC (2.5:1.5) 4000 3 20% compound of formula (I)·flonicamid SC (12:8) 4000 4 36% compound of formula (I)·flonicamid WP (18:18) 4000 5 10% hydroprene SC 2000 6 40% flonicamid SC 2000 7 Water treatment (CK) /

[0164] Test design: 6 pesticide treatments and 1 water treatment (CK) are set up, and a random arrangement is adopted, 3 repetitions for each treatment, 15 bean plants for each treatment, and an electric sprayer is used to spray the whole plant of the bean, and the front and back of the leaf is sprayed, and the water consumption is 45 L per mu.

[0165] Investigation frequency and time: the base number of T. urticae on each bean plant is investigated before the pesticide application, and the number of residual mites on the plant is investigated 1 d, 3 d, 14 d and 28 d after the pesticide application, and the mite reduction rate and control effect are calculated.

[0166] Data statistics and analysis: Duncan's new range test is used for the significant difference analysis.

[0167] Pharmacodynamic calculation method: the efficacy is calculated according to the following formula:

[0168]

[0169]

[0170] Preventive effect of different treatments on Tetranychus urticae on bean

[0171] Test results:

[0172] The preventive effect of each treatment on Tetranychus urticae is shown in Table 5 and Table 6. The four mixed formulations all show high rapidity and persistence, with control effect above 80%. The control effect of 20% compound (I)·flufenprox suspension concentrate (12:8) is 93.01% at 3 days after treatment, and the control effect is significant.

[0173] Table 5 Preventive effect of different treatments on Tetranychus urticae on bean (rapidity)

[0174]

[0175] Note: The number of Tetranychus urticae (head) and control effect (%) in the above table are the average values of each repetition. Lowercase letters represent significant differences at the 5% level.

[0176] Table 6 Preventive effect of different treatments on Tetranychus urticae on bean (persistence)

[0177]

[0178] Note: The number of Tetranychus urticae (head) and control effect (%) in the above table are the average values of each repetition. Lowercase letters represent significant differences at the 5% level.

[0179] And no phytotoxicity of the mixed formulations was found in the test, and the acaricidal composition or formulation can reduce production and use costs, and is safe to crops. The above tests show that each mixed treatment has good control effect on Tetranychus urticae, with good rapidity and persistence. It is found in the test process that each mixed treatment has no toxicity to natural enemies, and no leaf rolling, leaf burning and other phytotoxicity symptoms occur in the host plants during application.

[0180] Field test example 2 Field control test of Tetranychus urticae on eggplant

[0181] Test crop: eggplant, variety: Dalong;

[0182] Test object: Tetranychus urticae;

[0183] Test site: vegetable planting base in Qingzhou City, Weifang City, Shandong Province, with good management level and uniform growth. No pesticides were used after emergence to before application. The application period is the fruit setting period, and Tetranychus urticae occurs heavily.

[0184] Tested agents:

[0185] Table 7 Test treatment and dosage

[0186]

[0187]

[0188] Test design: The test was designed to have 6 agent treatments and 1 water treatment (CK), with random arrangement, 3 replicates for each treatment, 10 eggplants per treatment, spraying the whole plant with a sprayer, both sides of the leaves were sprayed, and the water usage was 45 L per mu.

[0189] Investigation frequency and time: The base number of two-spotted spider mites on 4 mature leaves of each eggplant was investigated before application, and the number of residual mites on the plants was investigated 1 d and 7 d after application to calculate the mite reduction rate and control effect.

[0190] Data statistics and analysis: Duncan's new multiple range test was used for significant difference analysis.

[0191] Method for calculating efficacy: Efficacy was calculated according to the following formula:

[0192]

[0193]

[0194] Control effect of different treatments on T. urticae

[0195] Test results:

[0196] The control effect of each treatment on T. urticae is shown in Table 8. 14% compound (I)·lufenuron suspension concentrate (10:4), 4% compound (I)·lufenuron emulsifiable concentrate (2.5:1.5), 20% compound (I)·fluazinam suspension concentrate (12:8), and 36% compound (I)·fluazinam wettable powder (18:18) all showed high control effect on T. urticae. 1 d after application, the control effects were 86.33%, 84.15%, 87.38%, and 81.04%, respectively; 7 d after application, the control effects were 89.02%, 85.36%, 86.44%, and 83.73%, respectively; and they all showed good speed and persistence compared with the control.

[0197] Table 8 Control effect of different treatments on T. urticae

[0198]

[0199] Note: The T. urticae (head) and control effect (%) in the above table are the average values of each replicate. Lowercase letters represent 5% level of significant difference.

[0200] During the experiment, it was found that each mixed treatment had no toxicity to natural enemies and was safe to eggplant during application, without symptoms of leaf rolling and leaf burning.

[0201] Although the present application has been described in detail with general description and specific embodiments above, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art, and therefore, these modifications or improvements made without departing from the spirit of the present application shall fall within the scope of the present application.

Claims

1. A mite-killing composition, characterized in that: The acaricidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I): (I) Active ingredient B is either lufenuron or fluazinam; the mass ratio of the compound of formula (I) to lufenuron is 1:24 to 24:1; the mass ratio of the compound of formula (I) to fluazinam is 1:20 to 24:

1.

2. The acaricide composition according to claim 1, characterized in that, The mass ratio of the compound of formula (I) to lufenuron is 1:24 to 7:1; the mass ratio of the compound of formula (I) to fluazinam is 1:15 to 5:

1.

3. The acaricide composition according to claim 1, characterized in that, The mass ratio of the compound of formula (I) to lufenuron is 1:24, 1:18, 1:14, 1:7, 5:2, 7:1, 15:1, 20:1, or 24:1; The mass ratio of the compound of formula (I) to fluoroquinolone is 1:20, 1:15, 1:10, 1:5, 3:2, 5:1, 12:1, or 24:

1.

4. The acaricide composition according to claim 1, characterized in that, Based on a total weight of 100 wt% of the acaricide composition, the sum of the contents of active ingredient A and active ingredient B in the acaricide composition is 5-80 wt%.

5. The acaricide composition according to claim 1, characterized in that, In addition to the active ingredient, the acaricide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

6. The acaricide composition according to claim 5, characterized in that, The acaricide composition is prepared into an agriculturally permissible formulation, wherein the formulation is selected from wettable powder, water-dispersible granules, soluble concentrate, emulsifiable concentrate, water emulsion, suspension concentrate, suspension emulsion, microemulsion, and dispersible oil suspension.

7. The application of the acaricidal composition according to any one of claims 1-6 in the control of harmful mites, characterized in that, The pest mentioned is the two-spotted spider mite.

8. The application according to claim 7, characterized in that, The acaricide composition is applied at an effective dose to the mites that need to be controlled or to the medium in which they grow.

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