Microbial feed additive for resisting livestock and poultry breeding based on bacterium-enzyme interaction principle

Abstract

The invention relates to a microbial feed additive for resisting livestock and poultry breeding based on a bacterium-enzyme interaction principle, which comprises a functional microbial feed additive and an enzyme-producing microbial feed additive, and the addition amount of the functional microbial feed additive and the enzyme-producing microbial feed additive accounts for 10% of the total weight of the feed. The addition ratio of the functional microorganism feed additive to the enzyme-producing microorganism feed additive is 1: 1. The application has the effects of reducing the incidence rate of enteritis and diarrhea and effectively reducing the dosage of fluoroquinolone and beta-lactam antibiotics.

Description

Technical Field

[0001] The present application relates to the field of feed additives, and in particular to a microbial feed additive for antibiotic replacement in livestock and poultry breeding based on the principle of bacteria-enzyme cooperation. Background Art

[0002] Antibiotic-free farming means that livestock and poultry are raised with the use of or without the use of antibiotics, with the purpose of protecting the health of livestock and poultry and people, and ultimately producing healthy, nutritious livestock products without antibiotic residues.

[0003] Many farmers choose to feed livestock and poultry with antibiotics because they can treat diseases, prevent infections, and promote growth. Antibiotics are effective quickly and can bring good economic benefits to farms. In addition, many farmers lack breeding knowledge, so they become dependent on antibiotics and use antibiotics beyond the limit.

[0004] Antibiotic-free farming can be divided into three levels: The first level means that no antibiotics can be detected when livestock and poultry are slaughtered; The second level refers to the process of feeding, which ensures that the feed does not contain antibiotics, hormones and other exogenous drugs and additives. They are allowed to be used in treatment, but a sufficient withdrawal period is guaranteed. The third level means that antibiotics, hormones and other exogenous drugs are not added to the feed during the breeding process, and they are not allowed to be used for treatment. At present, through the combined use of the combination, we can achieve the replacement of antibiotics in the feed and the entire process of enteritis and diarrhea treatment during the breeding process, and realize antibiotic-free breeding.

[0005] Microbial feed additives mainly work by bacterial enzymes to regulate the intestines and strengthen the key nutrients needed by animals. By reducing the use of antibiotics, healthy, nutritious livestock and poultry products without antibiotic residues are ultimately produced. Summary of the invention

[0006] In order to reduce the use of antibiotics and reduce antibiotic residues in livestock and poultry products, the present application provides a microbial feed additive and a preparation method for antibiotic-free livestock and poultry breeding based on the principle of bacteria-enzyme synergy.

[0007] The present application provides a microbial feed additive for antibiotic replacement livestock and poultry breeding based on the principle of bacteria-enzyme synergy, which adopts the following technical solution: A microbial feed additive for antibiotic replacement in livestock and poultry breeding based on the principle of bacteria-enzyme cooperation, comprising a functional microbial feed additive and an enzyme-producing microbial feed additive, wherein the addition amount of the functional microbial feed additive and the enzyme-producing microbial feed additive accounts for 10% of the total weight of the feed, and the addition ratio of the functional microbial feed additive and the enzyme-producing microbial feed additive is 1:1.

[0008] By adopting the above technical scheme, using functional microbial feed additives and enzyme-producing microbial feed additive compositions for livestock and poultry breeding and feeding can reduce the incidence of enteritis and diarrhea by more than 90%, and effectively reduce the dosage of fluoroquinolone and β-lactam antibiotics.

[0009] Optionally, the functional microbial feed additive is obtained by mixed fermentation of Clostridium butyricum, Saccharomyces cerevisiae and Lactobacillus plantarum.

[0010] By adopting the above technical solutions, the specifically developed compound bacterial agents can be used to prevent gastrointestinal diseases in animals, such as preventing and treating diarrhea in farmed animals and reducing the amount of medication.

[0011] Optionally, the culture medium components of the functional microbial feed additive are brown sugar, corn starch, sucrose, corn flour, glycerol, potassium dihydrogen phosphate, peptone, yeast powder, beef extract, glucose, trace elements, growth factors, and vitamins, and the trace elements are one or more of iron sulfate, zinc sulfate, calcium chloride, manganese sulfate, sodium acetate, triammonium citrate, ferric chloride, calcium sulfate, and magnesium chloride.

[0012] Optionally, the enzyme-producing microbial feed additive is obtained by mixed fermentation of Bacillus amyloliquefaciens and Bacillus licheniformis.

[0013] By adopting the above technical scheme, the enzyme-producing microbial feed additive also has a nutritional effect, which promotes the growth of animals. The beneficial bacteria in the direct-fed microbial feed can be metabolized in the intestine to produce a variety of beneficial factors that help animals digest nutrients, thereby promoting the growth, development and weight gain of livestock and poultry.

[0014] Optionally, the solid culture medium components of the enzyme-producing microbial feed additive are corn starch, corn flour, soybean flour, rice husk powder, potassium dihydrogen phosphate, yeast peptone, yeast powder, beef extract, glucose, trace elements, growth factors, and vitamins, and the trace elements are one or more of iron sulfate, zinc sulfate, calcium chloride, manganese sulfate, sodium acetate, triammonium citrate, ferric chloride, calcium sulfate, and magnesium chloride.

[0015] Optionally, the microbial culture tank includes a tank body, an opening at the upper end of the tank body is connected to a tank top via a flange, a material guide port is provided on the tank top, a closing cover is connected to the material guide port via a flange, a discharge pipe is fixedly connected to the lower end of the tank body, a switch valve is fixedly connected to the discharge pipe, a spacing mechanism is vertically fixedly connected in the tank body, and a flow pushing mechanism is arranged in the spacing mechanism.

[0016] Optionally, the spacing mechanism includes a spacing cylinder coaxially arranged with the tank body, the spacing cylinder is located in the tank body, the lower end of the spacing cylinder is fixedly connected to a circular closing plate, two guide ports are radially opened on the closing plate along the closing plate, the two guide ports are spaced apart, and the length direction of the guide ports is radially parallel to the closing plate, the flow pushing mechanism includes a guide cylinder fixedly connected to the inner wall of the spacing cylinder, a pushing assembly is arranged in the guide cylinder, the lower end of the spacing cylinder is connected to a blocking assembly for blocking the guide port through a connecting assembly, and a bearing assembly is arranged on the guide cylinder which moves synchronously with the pushing assembly.

[0017] Optionally, the sealing assembly includes a fixed rod vertically fixedly connected to the lower surface of the second driving ring, a sealing plate is hingedly connected to the end of the fixed rod away from the second driving ring, and a sliding rod is horizontally fixedly connected to the end of the sealing plate away from the second fixed rod, the length direction of the sliding rod is parallel to the length direction of the second fixed rod, and a sliding groove is opened on the side wall in the length direction of the guide port along the length direction of the guide port, and the end of the sliding rod close to the sliding groove is slidably connected to the corresponding sliding groove, and when the sliding rod slides to the end of the sliding groove close to the inner wall of the spacing cylinder, the sealing plate closes the guide port.

[0018] Optionally, the pushing assembly includes a support plate fixedly connected to the bottom of the guide tube, a driving motor fixedly connected to the tank top, a driving screw rotatably connected to the support plate, the driving screw and the guide tube are coaxially arranged, one end of the driving screw is rotatably connected to the support plate, and the other end passes through the tank top and is coaxially fixedly connected to the driving motor, a driving block is threadedly connected to the driving screw, the driving block is cylindrical in design, the outer wall of the driving block contacts the inner wall of the guide tube, the driving block is axially slidable along the guide tube and is connected to the guide tube, and when the driving block descends, the sealing plate slides to open the guide port.

[0019] In summary, the present application includes at least one of the following beneficial technical effects: 1. The role of microbial fermented feed is to use cheap agricultural and light industrial by-products to produce high-quality feed protein raw materials, improve feed utilization, and reduce breeding costs. The application of microorganisms can activate digestive enzymes to increase feed conversion rates, promote the growth of livestock and poultry, and regulate the microecological balance of animals to improve the health of animals.

[0020] 2. Microbial fermentation feed uses bacteria and enzymes to carry out antibiotic-free breeding, replacing the use of antibiotics and improving the quality of meat and egg products. Targeted compound bacterial agents can be used to prevent gastrointestinal diseases in animals, such as preventing and treating diarrhea in farmed animals, and reducing the amount of medication. The promotion and application of the principle of bacteria and enzymes in livestock and poultry breeding can reduce the use of antibiotics, which is conducive to the production of safe food and improves the competitiveness of product exports.

[0021] 3. The use of functional microbial feed additives and enzyme-producing microbial feed additive compositions for livestock and poultry feeding can reduce the incidence of enteritis and diarrhea by more than 90%, and effectively reduce the dosage of fluoroquinolones and β-lactam antibiotics.

[0022] 4. Due to the interaction between microorganisms, the existing microorganisms in an ecosystem will prevent the invasion of new organisms in this area. Beneficial bacteria prevent harmful microorganisms from adhering to and multiplying in the intestinal mucosa through competitive inhibition. Therefore, it is very important for young animals with slow or impaired flora formation to take microbial feed. Lactobacillus plantarum can produce a small amount of hydrogen peroxide, which can inhibit the growth of many bacteria, especially Gram-negative pathogens. The extracellular glycosidase produced by lactobacillus can prevent bacterial toxins from adhering to and invading epithelial cells.

[0023] 5. Enzyme-producing microbial feed additives also have nutritional effects and promote animal growth. Beneficial bacteria in direct feeding microbial feed can metabolize in the intestine to produce a variety of beneficial factors that help animals digest nutrients, thereby promoting the growth and weight gain of livestock and poultry. For example, probiotics such as Saccharomyces cerevisiae can secrete a variety of digestive enzymes, such as protease, amylase, lipase, etc. to promote the digestion and absorption of nutrients; amino acids, vitamins K, C, B1, B2, pantothenic acid, niacin, biotin, inositol and folic acid produced by yeast; Saccharomyces cerevisiae can also promote the production of phytase and improve the utilization rate of phosphorus by monogastric animals. Improve the body's immune function. Beneficial microorganisms in microbial fermented feed can act as a non-specific immune regulatory factor, stimulate and activate host immune cells through the bacteria themselves or cell wall components, promote phagocyte activity or act as an adjuvant. Beneficial microorganisms can also play a specific immune function and enhance the ability of B cells in animals to produce antibodies. Direct feeding microorganisms promote humoral immunity and cellular immunity in the body, improve the antibody level of livestock and poultry, enhance immune function, and kill pathogenic bacteria invading the body in time, thereby preventing the occurrence of diseases.

[0024] 6. It can be fed throughout the whole process, and functional microbial feed additives can be added at 5% throughout the whole process. For the treatment of livestock and poultry with enteritis and diarrhea symptoms, 5% enzyme-producing microbial feed additives are added, and livestock and poultry can be gavage-fed in the early stage of the disease. Through functional microbial feed additives and enzyme-producing microbial feed additives, it can be ensured that enteritis and diarrhea symptoms in all stages of livestock and poultry breeding can be carried out without antibiotics. Functional microbial feed additives are used to improve immunity and enzyme-producing microbial feed additives are mainly used for the prevention and control of enteritis and diarrhea. Both do not contain antibiotics, hormones, and other exogenous drugs and additives, and do not contain any antibiotics or hormone ingredients in the treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1It is a schematic diagram of the overall structure of Example 3 of the present application; Figure 2 It is a schematic cross-sectional view of part of the structure of Example 3 of the present application; Figure 3 It is a schematic cross-sectional view of part of the structure of Example 3 of the present application; Figure 4 It is a schematic cross-sectional view of part of the structure of Example 3 of the present application; Figure 5 It is a schematic cross-sectional view of part of the structure of Example 3 of the present application; Figure 6 It is a schematic cross-sectional view of part of the structure of Example 3 of the present application.

[0026] Explanation of reference numerals: 1, tank body; 11, discharge pipe; 2, tank top; 21, guide port; 3, spacing mechanism; 31, spacing cylinder; 32, first support rod; 33, second support rod; 34, closing plate; 341, flow guide port; 4, flow pushing mechanism; 41, flow guide cylinder; 411, sliding through hole; 42, pushing assembly; 421, support plate; 422, driving motor; 423, driving screw; 424, driving block; 4241, air guide hole; 425, guide rod; 426, first telescopic sleeve; 427, second telescopic sleeve; 43, connecting assembly; 431, first driving ring; 432, second driving ring; 433, support rod; 434, driving gear; 435, first driving rod; 436, first driving rack; 437, second driving rod; 438, second driving rack; 44. Sealing assembly; 441. Fixed rod; 442. Sealing plate; 443. Sliding rod; 444. Sliding groove; 45. Bearing assembly; 451. Bearing ring; 451. Mounting through hole; 452. Bearing net. DETAILED DESCRIPTION

[0027] This application is further described in detail in conjunction with the following contents.

[0028] Preparation Example 1 Preparation Example 1 of the present application discloses a functional bacterial strain and culture medium.

[0029] Clostridium butyricum: yeast extract powder 3g / L, beef extract 10g / L, peptone 10g / L, soluble starch 1g / L, glucose 5g / L, cysteine ​​hydrochloride 0.5g / L, sodium chloride 3g / L and sodium acetate 3g / L; anaerobic culture.

[0030] Preparation Example 2 Preparation Example 2 of the present application discloses a functional bacterial strain and culture medium.

[0031] Brewer's yeast: 200 g / L potatoes, 20 g / L glucose, 20 g / L agar (added as needed). Boil the potatoes, filter out the filtrate, add distilled water in sequence and make up to 1000 mL, sterilize at 121°C, 0.1 MPa for 30 min.

[0032] Preparation Example 3 Preparation Example 3 of the present application discloses a functional bacterial strain and culture medium.

[0033] Lactobacillus plantarum: Add 10.0 g of peptone, 10.0 g of beef extract, 5.0 g of yeast extract, 20.0 g of glucose, 5.0 g of sodium acetate, 2.0 g of diammonium citrate, 1.0 g of Tween-80, 2 g of dipotassium hydrogen phosphate, 0.58 g of magnesium sulfate, 0.25 g of manganese sulfate, 20.0 g of calcium carbonate, and 20 g / L of agar (added as needed) into distilled water in sequence and make up to 1000 mL of distilled water. Sterilize at 121°C, 0.1 MPa for 30 min.

[0034] Preparation Example 4 Preparation Example 4 of the present application discloses an enzyme-producing bacterial strain and culture medium.

[0035] Bacillus licheniformis, producing acidic protease: peptone 10g / L, beef extract 3g / L, sodium chloride 5g / L, PH7.0-7.2, 35 degrees Celsius, 180 rpm, 8-10 hours.

[0036] Protease enzyme solid fermentation medium: material-water ratio is 1:0.6. In the solid material, wheat bran: soybean powder: rice husk powder is 1:0.6:0.2, the culture temperature is 40-43℃, the fermentation time is 65-72h, pH is 5.5-6.5, and the inoculation amount is 10%. Under the optimal culture conditions, the acid protease activity is measured to be 1029.5U / g.

[0037] Preparation Example 5 Preparation Example 5 of the present application discloses an enzyme-producing bacterial strain and culture medium.

[0038] Bacillus amyloliquefaciens, producing medium-temperature amylase: peptone 10g / L, beef extract 3g / L, sodium chloride 5g / L, PH7.0-7.2, 37 degrees Celsius, 180 rpm, 10-12 hours.

[0039] Solid fermentation medium for amylase production: the material-water ratio is 1:0.6. In the solid material, the ratio of wheat bran: corn flour: soybean flour is 1:0.4:0.2, the culture temperature is 35-60℃, the fermentation time is 50-60h, the pH is 5.5-6.5, and the inoculation amount is 10%. Under the optimal culture conditions, the stable amylase activity is 60.5U / g. Example

[0040] The present application example discloses a functional microbial feed additive, which is prepared by the following steps: The activated Clostridium butyricum, Saccharomyces cerevisiae and Lactobacillus plantarum were taken respectively and transferred to corresponding liquid shaking bottles to prepare seed liquid, wherein Saccharomyces cerevisiae and Lactobacillus plantarum were cultured facultatively anaerobically, and Clostridium butyricum was cultured absolutely anaerobically.

[0041] After the shake flask of Clostridium butyricum is inoculated, it is cultured and reaches the middle or late logarithmic growth phase, which means it has reached the standard for inoculating the seed tank.

[0042] After inoculation of Lactobacillus plantarum and Saccharomyces cerevisiae, the shake flasks are cultured, and the growth curve OD600 is tested every two hours after inoculation. The growth curve is drawn and reaches the middle or late stage of the logarithmic growth phase, which means that the standard for inoculating the seed tank has been met.

[0043] Clostridium butyricum was purified, activated and cultured using RCM medium, Saccharomyces cerevisiae was purified using PDA medium, and Lactobacillus plantarum was purified, activated and cultured using MRS finished solid medium.

[0044] Step 1: The components of the culture medium in the microbial fermentation tank are brown sugar, corn starch, sucrose, corn flour, glycerol, potassium dihydrogen phosphate, peptone, yeast powder, beef extract, glucose, trace elements, growth factors, vitamins, etc. Among them, vitamins need to be filtered and sterilized, and trace elements are one or more of iron sulfate, zinc sulfate, calcium chloride, manganese sulfate, sodium acetate, triammonium citrate, iron trichloride, calcium sulfate, and magnesium chloride. The sterilization temperature of the culture medium is 121°C. Steam sterilization for 30 to 40 minutes. The sterilization pressure is 0.1 to 0.15Mpa, 180r / min, 32°C mixed fermentation for 3-4 days.

[0045] The second step is to use multi-strain fusion symbiosis technology to control the multi-strain fusion symbiosis technology and prepare mixed fermentation liquid. Clostridium butyricum uses nitrogen to control the oxygen content in the fermentation tank to achieve anaerobic culture, and the temperature and speed of the three bacteria are fully automated.

[0046] Step 3: Stop canning standards. After 3-4 days of mixed fermentation of yeast and Lactobacillus plantarum, the number of effective live bacteria in each function is about 2 billion / ml or more, and canning can be stopped. Canning can be stopped when the spore production rate of Clostridium butyricum reaches more than 95%.

[0047] Step 4: Product compounding: For the products that have been fermented and have qualified effective live bacteria counts in step 3, yeast protein, complex amino acids, natural minerals, multivitamins, oligosaccharides and other nutrients are added to compound the products before packaging. Example

[0048] The present application example discloses an enzyme-producing microbial feed additive, which is prepared by the following steps: The activated Bacillus amyloliquefaciens and Bacillus licheniformis were taken separately and transferred to corresponding liquid shaking bottles to prepare seed liquid, and then inoculated into solid fermentation medium according to corresponding proportions for solid fermentation and enzyme production culture.

[0049] The first step is to culture the shake flasks after inoculation of Bacillus amyloliquefaciens and Bacillus licheniformis. After inoculation, the growth curve OD600 is tested every two hours, and the growth curve is drawn. When the middle or late logarithmic growth period is reached, the standard for inoculating the seed tank is reached.

[0050] The second step is that the solid culture medium components in the microbial fermentation tank are corn starch, corn flour, soybean flour, rice husk flour, potassium dihydrogen phosphate, yeast peptone, yeast powder, beef extract, glucose, trace elements, growth factors, vitamins, etc. Vitamins need to be filtered and sterilized, and trace elements are one or more of iron sulfate, zinc sulfate, calcium chloride, manganese sulfate, sodium acetate, ammonium citrate, ferric chloride, calcium sulfate, and magnesium chloride. The sterilization temperature of the culture medium is 121°C. Steam sterilization for 30 to 40 minutes. The sterilization pressure is 0.1 to 0.15Mpa, and the inoculation ratio is 10%. Under the respective processes, the solid fermentation is 3-4 days.

[0051] Step 3: Cultivation suspension standard: Under the optimum cultivation conditions, the amylase activity of Bacillus amyloliquefaciens is not less than 50 U / g, and the acid protease activity of Bacillus spp. under the optimum cultivation conditions is not less than 800 U / g.

[0052] Step 4: Product compounding. For the products that have been fermented and have qualified enzyme activity in the third step, corn starch, corn flour, soybean flour, rice husk flour, active probiotics, yeast protein, methionine, lysine, natural minerals, VC, glucose and other nutrients are added to the products for compounding and packaging. Example

[0053] The present application embodiment discloses a microorganism culture tank, combined with the attached Figure 1-6 Provide explanation.

[0054] The microorganism culture tank comprises a tank body 1, the upper opening of the tank body 1 is connected to a tank top 2 through a flange, a material guide port 21 is provided on the tank top 2, and a closing cover is connected to the material guide port 21 through a flange. A discharge pipe 11 is fixedly connected to the lower end of the tank body 1, and a switch valve is fixedly connected to the discharge pipe 11. A spacing mechanism 3 is vertically fixedly connected in the tank body 1, and a flow pushing mechanism 4 is arranged in the spacing mechanism 3.

[0055] The flow-pushing mechanism 4 pushes the material water in the spacing mechanism 3 to flow, thereby driving the material water in the tank body 1 to flow, effectively driving the solid materials in the material water to be evenly dispersed in the tank body 1, thereby improving the microbial culture effect.

[0056] The spacing mechanism 3 includes a spacing cylinder 31 coaxially arranged with the tank body 1, and the spacing cylinder 31 is located in the tank body 1. The lower end of the spacing cylinder 31 is vertically fixedly connected to a plurality of first support rods 32. In this embodiment, there are four first support rods 32, which are circumferentially arranged with the axis of the spacing cylinder 31 as the center.

[0057] Two groups of second support rods 33 are fixedly connected to the outer wall of the spacing cylinder 31 at axial intervals along the spacing cylinder 31, each group of second support rods 33 consists of four, and the four second support rods 33 in one group are equidistantly arranged along the circumference of the spacing cylinder 31, and the length direction of the second support rods 33 is radially parallel to the spacing cylinder 31, one end of the second support rods 33 is fixedly connected to the outer wall of the spacing cylinder 31, and the other end is fixedly connected to the inner wall of the tank body 1.

[0058] A circular closing plate 34 is fixedly connected to the lower end of the spacing cylinder 31 . Two guide ports 341 are opened in the radial direction of the closing plate 34 . The two guide ports 341 are spaced apart, and the length direction of the guide ports 341 is parallel to the radial direction of the closing plate 34 .

[0059] The flow pushing mechanism 4 includes a guide tube 41 fixedly connected to the inner wall of the spacing tube 31, a pushing assembly 42 is arranged in the guide tube 41, the lower end of the spacing tube 31 is connected to a blocking assembly 44 for blocking the guide port 341 through a connecting assembly 43, and the upper end of the guide tube 41 is provided with a bearing assembly 45 that moves synchronously with the pushing assembly 42.

[0060] The upper end of the guide tube 41 is higher than the upper end of the spacing tube 31 , and the height of the material water is located between the upper end of the guide tube 41 and the upper end of the spacing tube 31 .

[0061] The push assembly 42 includes a support plate 421 fixedly connected to the bottom of the guide tube 41, and a drive motor 422 fixedly connected to the tank top 2. A drive screw 423 is rotatably connected to the support plate 421, and the drive screw 423 is coaxially arranged with the guide tube 41. One end of the drive screw 423 is rotatably connected to the support plate 421, and the other end passes through the tank top 2 and is coaxially fixedly connected to the drive motor 422. A drive block 424 is threadedly connected to the drive screw 423, and the drive block 424 is cylindrical in design. The outer wall of the drive block 424 contacts the inner wall of the guide tube 41, and the drive block 424 is axially slidable along the guide tube 41 and connected to the guide tube 41.

[0062] A guide rod 425 is fixedly connected to the outer wall of the driving block 424. Two guide rods 425 are arranged circumferentially along the outer wall of the driving block 424. A sliding through hole 411 is opened along the axial direction of the guide tube 41 on the side wall of the guide tube 41 corresponding to the sliding track of the guide rod 425. The guide rod 425 is slidably connected in the sliding through hole 411.

[0063] The driving screw 423 is sleeved with a first telescopic sleeve 426 and a second telescopic sleeve 427, and the driving block 424 is located between the first telescopic sleeve 426 and the second telescopic sleeve 427. One end of the first telescopic sleeve 426 is fixedly connected to the support plate 421, and the other end is fixedly connected to the driving block 424. One end of the second telescopic sleeve 427 is fixedly connected to the tank top 2, and the other end is fixedly connected to the driving block 424. The driving block 424 is provided with an air guide hole 4241, which is located within the coverage of the first telescopic sleeve 426 and the second telescopic sleeve 427.

[0064] The connecting assembly 43 includes a first drive ring 431 and a second drive ring 432 coaxially sleeved on the outer wall of the guide tube 41. The first drive ring 431 is located above the second drive ring 432. The first drive ring 431 and the second drive ring 432 are spaced apart. The guide rod 425 is fixedly connected to the first drive ring 431.

[0065] The inner wall of the guide tube 41 is fixedly connected with a support rod 433, and the support rod 433 is located between the first drive ring 431 and the second drive ring 432. The support rod 433 is rotatably connected with a driving gear 434, and the axis of the driving gear 434 is perpendicular to the axis of the guide tube 41 and is arranged horizontally.

[0066] A first driving rod 435 is vertically fixedly connected to the lower surface of the first driving ring 431 , and a first driving rack 436 is vertically fixedly connected to the side wall of the first driving rod 435 . The first driving rack 436 is meshed with the driving gear 434 .

[0067] A second driving rod 437 is vertically fixedly connected to the upper surface of the second driving ring 432 , a second driving rack 438 is vertically fixedly connected to the side wall of the second driving rod 437 , and the second driving rack 438 is meshed with the driving gear 434 .

[0068] The blocking assembly 44 includes a fixed rod 441 vertically fixedly connected to the lower surface of the second driving ring 432, a blocking plate 442 is hingedly connected to one end of the fixed rod 441 away from the second driving ring 432, and a sliding rod 443 is horizontally fixedly connected to one end of the blocking plate 442 away from the second fixed rod 442. The length direction of the sliding rod 443 is parallel to the length direction of the second fixed rod 442. The side wall of the guide port 341 in the length direction is provided with a sliding groove 444 along the length direction of the guide port 341, and the end of the sliding rod 443 close to the sliding groove 444 is slidably connected to the corresponding sliding groove 444. When the sliding rod 443 slides to the end of the sliding groove 444 close to the inner wall of the spacing cylinder 31, the blocking plate 442 closes the guide port 341.

[0069] The bearing assembly 45 includes a mounting through hole 451 formed on the first driving ring 431 , and a bearing net is fixedly connected in the mounting through hole 451 .

[0070] The implementation principle of a microorganism culture tank in Example 3 of the present application is as follows: after the driving motor 422 is started, the driving screw 423 drives the driving block 424 to slide downward, and at this time, the driving block 424 drives the first driving ring 431 to descend through the guide rod 425. The first driving ring 431 drives the driving gear 434 to rotate through the first driving rod 435 and the first driving rack 436, and then drives the second driving rack 438 and the second driving rod 437 to slide, so as to realize the rise of the second driving ring 432. While the second driving ring 432 rises, it drives the blocking plate 442 to slide through the fixing rod 441 and the second fixing rod 442, so as to leak the guide port 341.

[0071] During the gradual opening of the diversion port 341 , the water flow generated by the driving block 424 lifts up the solid materials such as corn flour deposited at the bottom of the tank body 1 , thereby moving to the upper end of the tank body 1 and entering the spacing cylinder 31 from the top of the spacing cylinder 31 .

[0072] When the driving block 424 rises, the driving block 424 drives the carrying net 452 to rise through the first driving ring 431, so that the solid material washed to the top of the spacing cylinder 31 is hung on the carrying net 452. When the carrying net 452 descends with the first driving ring 431, the solid material on the carrying net 452 is washed off and scattered in the material water, thereby effectively improving the dispersion uniformity of the solid material in the tank body 1 and improving the microbial culture effect.

[0073] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A microbial feed additive for antibiotic replacement in livestock and poultry breeding based on the principle of bacteria-enzyme cooperation, characterized by: It comprises functional microbial feed additives and enzyme-producing microbial feed additives, wherein the addition amount of the functional microbial feed additives and the enzyme-producing microbial feed additives accounts for 10% of the total weight of the feed, and the addition ratio of the functional microbial feed additives and the enzyme-producing microbial feed additives is 1:

1.

2. The microbial feed additive for antibiotic replacement in livestock and poultry breeding based on the bacteria-enzyme synergy principle according to claim 1, characterized in that: The functional microbial feed additive is obtained by mixed fermentation of Clostridium butyricum, Saccharomyces cerevisiae and Lactobacillus plantarum in a microbial culture tank.

3. The microbial feed additive for antibiotic replacement in livestock and poultry breeding based on the bacteria-enzyme synergy principle according to claim 2, characterized in that: The culture medium components of the functional microbial feed additive are brown sugar, corn starch, sucrose, corn flour, glycerol, potassium dihydrogen phosphate, peptone, yeast powder, beef extract, glucose, trace elements, growth factors, and vitamins. The trace elements are one or more of ferric sulfate, zinc sulfate, calcium chloride, manganese sulfate, sodium acetate, triammonium citrate, ferric chloride, calcium sulfate, and magnesium chloride.

4. The microbial feed additive for antibiotic replacement in livestock and poultry breeding based on the bacteria-enzyme synergy principle according to claim 3, characterized in that: The enzyme-producing microbial feed additive is obtained by mixed fermentation of Bacillus amyloliquefaciens and Bacillus licheniformis in a microbial culture tank.

5. The microbial feed additive for antibiotic replacement in livestock and poultry breeding based on the bacteria-enzyme synergy principle according to claim 4, characterized in that: The solid culture medium components of the enzyme-producing microbial feed additive are corn starch, corn flour, soybean flour, rice husk flour, potassium dihydrogen phosphate, yeast peptone, yeast powder, beef extract, glucose, trace elements, growth factors, and vitamins. The trace elements are one or more of ferric sulfate, zinc sulfate, calcium chloride, manganese sulfate, sodium acetate, triammonium citrate, ferric chloride, calcium sulfate, and magnesium chloride.

6. A microorganism culture tank for use in claim 2 or 4, characterized in that: The microorganism culture tank comprises a tank body (1), the upper opening of the tank body (1) is connected to a tank top (2) via a flange, a material guide port (21) is provided on the tank top (2), a sealing cover is connected to the material guide port (21) via a flange, a discharge pipe (11) is fixedly connected to the lower end of the tank body (1), a switch valve is fixedly connected to the discharge pipe (11), a spacing mechanism (3) is vertically fixedly connected inside the tank body (1), and a flow pushing mechanism (4) is arranged inside the spacing mechanism (3).

7. The microorganism culture tank according to claim 6, characterized in that: The spacing mechanism (3) comprises a spacing cylinder (31) coaxially arranged with the tank body (1), the spacing cylinder (31) being located inside the tank body (1), the lower end of the spacing cylinder (31) being fixedly connected to a circular closing plate (34), two flow guide openings (341) being radially arranged on the closing plate (34), the two flow guide openings (341) being spaced apart, the length direction of the flow guide openings (341) being parallel to the radial direction of the closing plate (34), the flow pushing mechanism (4) comprising a flow guide cylinder (41) fixedly connected to the inner wall of the spacing cylinder (31), a pushing assembly (42) being arranged inside the flow guide cylinder (41), the lower end of the spacing cylinder (31) being connected to a blocking assembly (44) for blocking the flow guide opening (341) via a connecting assembly (43), and a bearing assembly (45) for moving synchronously with the pushing assembly (42) being arranged on the flow guide cylinder (41).

8. The microorganism culture tank according to claim 7, characterized in that: The blocking assembly (44) comprises a fixing rod (441) vertically fixedly connected to the lower surface of the second driving ring (432); a blocking plate (442) is hingedly connected to one end of the fixing rod (441) away from the second driving ring (432); a sliding rod (443) is horizontally fixedly connected to one end of the blocking plate (442) away from the second fixing rod (442); the length direction of the sliding rod (443) is parallel to the length direction of the second fixing rod (442); a sliding groove (444) is provided on the side wall of the guide port (341) in the length direction along the length direction of the guide port (341); an end of the sliding rod (443) close to the sliding groove (444) is slidably connected to the corresponding sliding groove (444); when the sliding rod (443) slides to one end of the sliding groove (444) close to the inner wall of the spacing cylinder (31), the blocking plate (442) closes the guide port (341).

9. The microorganism culture tank according to claim 7, characterized in that: The pushing assembly (42) comprises a support plate (421) fixedly connected to the bottom of the guide tube (41); a driving motor (422) is fixedly connected to the tank top (2); a driving screw (423) is rotatably connected to the support plate (421); the driving screw (423) is coaxially arranged with the guide tube (41); one end of the driving screw (423) is rotatably connected to the support plate (421); the other end passes through the tank top (2) and is coaxially fixedly connected to the driving motor (422); a driving block (424) is threadedly connected to the driving screw (423); the driving block (424) is cylindrical in design; an outer wall of the driving block (424) contacts an inner wall of the guide tube (41); the driving block (424) is slidably connected to the guide tube (41) along the axial direction of the guide tube (41); when the driving block (424) descends, the blocking plate (442) slides to open the guide port (341).

Images