Fermented feed composition for improving immunity of Qingyuan chickens as well as preparation method and application of fermented feed composition

Abstract

The invention relates to the technical field of feed production, and discloses a fermented feed composition for improving immunity of Qingyuan chickens as well as a preparation method and application thereof, and the preparation method specifically comprises the following steps: S1, mixing chlorella, kelp, brown algae and spirulina, grinding into mud, then mixing sodium humate and sodium alginate to prepare algae mud, and mixing the algae mud with cereal particles to obtain a mixed material; s2, the mixed material and fermentation protein are mixed and then subjected to ventilation fermentation, then closed fermentation is conducted in a fermentation tank to obtain a fermentation material, protein carrying strains and organic salt can form a polymer system through electrostatic interaction to stabilize and protect the strains, and substances can be efficiently fermented and decomposed through a secondary fermentation technology; s3, performing enrichment treatment on the fermented material, further eliminating and solidifying active substances, and performing vacuum low-temperature drying to obtain the fermented feed composition with high nutritional ingredients, high-activity probiotics and low resistance, and the fermented feed composition can be applied to effectively improving the body performance and immunity of Qingyuan chickens.

Description

Technical Field

[0001] The invention relates to the technical field of feed production, and in particular to a fermented feed composition capable of improving the immunity of Qingyuan chickens, and a preparation method and application thereof. Background Art

[0002] Qingyuan chicken is produced in the north-central part of Guangdong Province and the middle and lower reaches of Beijiang River. It is located in the junction of the Pearl River Delta and the mountainous area in northern Guangdong. The area has a subtropical monsoon climate, is mild and rainy all year round, and has many hills and vegetation. Therefore, the oxygen content is high and the water quality is excellent. There are also abundant natural feed raw materials. Insects, river fish and vegetation seeds are all the favorite food materials of Qingyuan chicken. The Qingyuan chicken fed with this food has firm and tender meat, delicious taste and rich nutrients. It is rich in selenium, vitamins, unsaturated fatty acids and other trace elements that are beneficial to health. It has efficacy and can enhance the body's immunity and prevent diseases.

[0003] However, the current promotion of digital farming can achieve large-scale production of broiler chickens to meet market demand, but the high incidence of diseases in farming seriously affects the benefits of farming. In order to ensure the quality and health of chicken, it is not advisable to use antibiotics and hormones to improve the disease resistance of chickens, but should follow the green concept; fermented feed generally contains rich probiotics and rich nutrients produced by fermentation, and has the advantages of being easy to digest and absorb. Compared with general feed, fermented feed has lower toxin content due to degradation, which can effectively improve the immunity of farmed chickens; but the current fermented feed cannot significantly improve the nutritional value of the feed, and it is easy for bacteria to compete with each other, and it is easy to produce some harmful substances to endanger animal health. At the same time, it is not easy to store, and the excessive content of microorganisms in it can easily cause secondary fermentation and corruption. Summary of the invention

[0004] In view of the above problems, the object of the present invention is to provide a fermented feed composition for improving the immunity of Qingyuan chickens, and a preparation method and application thereof.

[0005] The technical contents of the present invention are as follows: The present invention provides a method for preparing a fermented feed composition for improving the immunity of Qingyuan chickens, comprising the following steps: S1: mixing chlorella, giant kelp, brown algae and spirulina in a mass ratio of 9-13:4-8:1:1, grinding them into mud, mixing them with sodium humate and sodium alginate to prepare algae mud, and mixing them with cereal particles to obtain a mixed material; The addition amounts of sodium humate and sodium alginate are 4-8wt% and 10-16wt% respectively; Algae mud is rich in vitamins, phytochemicals and other trace elements. The lipid substances produced by algae decomposition can combine with bacterial amino acids and inactivate the functional groups of amino acids used to synthesize proteins, as well as break the bonds of proteins to inhibit the growth of bacteria. It can resist bacteria and viruses and enhance immunity. The cereal particles are obtained by mixing corn, straw, wheat bran, sunflower seeds and coconut shells and grinding them into a particle size of 1-3 mm; S2: The mixed material and the fermented protein are mixed and then subjected to secondary fermentation. The secondary fermentation is firstly subjected to ventilation fermentation, and then subjected to closed fermentation in a fermentation tank at a temperature of 30-40° C. for 6-9 days to obtain a fermented material. The conditions for ventilation fermentation are: 25-35° C., stirring at 150-180 rpm, continuous ventilation at a ventilation ratio of 0.4-0.7 vvm, and cultivation for 15-18 hours. The fermented protein is prepared by mixing protein raw materials consisting of soybean meal, hemp leaf bamboo shoot processing byproducts and palm meal in a mass ratio of 1:5-9:3, freeze-drying, extruding and puffing, and then fermenting with a mixed fermentation bacterial suspension in a solid-liquid ratio of 3:11-13 g / mL; The fermentation of the fermented protein is to mix the protein raw material with 3-6wt% of monocalcium phosphate and ferment it in a closed manner at a temperature of 30-50°C for 3-6h; The fermented protein utilizes local processing byproducts as protein resources, removes impurities and initial decomposition through temperature difference and pressure, and then ferments to act on the amide bonds and peptide bonds at the carboxyl end of the protein to break the aggregation of the protein, and optimizes the protein fermentation to obtain high-nutrition protein with low content of macromolecular proteins and high content of alcohol-soluble proteins, low-molecular peptides and other components; The fermentation bacteria suspension has a concentration of 1×10 5 ~1×10 8 CFU / g of Lactobacillus johnsonii BS15, 1×10 2 ~1×10 7 CFU / g of Lactobacillus casei, 1×10 5 CFU / g of Enterococcus faecalis and 1×10 8 CFU / g of Clostridium butyricum was mixed in the hydrolyzate; The hydrolyzate is a PBS buffer solution containing 10-16 wt% earthworm peptides; The nutrients such as short-chain fatty acids produced by the decomposition of probiotics in the fermented bacterial liquid can promote the proliferation of probiotics, and the earthworm peptides in the decomposition liquid can chelate the trace elements of algae and promote the proliferation of probiotics and assist the anaerobic fermentation reaction; The ventilation fermentation promotes the proliferation of Clostridium butyricum and can inhibit bacterial growth. Subsequently, the closed fermentation and Clostridium butyricum promote the proliferation of other anaerobic bacteria, decomposing and converting carbohydrates into immune substances such as lactic acid. At the same time, Enterobacteriaceae softens and decomposes fibers, which can reduce crude fiber content and increase water-soluble fiber content, regulate body functions, and improve metabolic capacity and immunity. The active ingredients of algae mud and fermented protein enter the grain particles and form an embedded material with a stable structure with the oil components and fibers therein. At the same time, the antioxidant components in the algae mud can form a semi-dehydrogenated oxygen-repelling structure under the action of the bacteria, which can also increase the stability of substances such as oils and fats. S3: enriching the fermented material, and then drying it at low temperature under vacuum conditions to obtain a fermented feed composition; The enrichment treatment is to first subject the fermented material to ultrasonic treatment at an ultrasonic frequency of 20-30 KHz for 10-20 minutes and then to electrostatic treatment at a field strength of 0.5-0.8 V / m for 11-18 minutes; The enrichment treatment decomposes the resistant components through the physical energy of ultrasound, increases the content of functional small molecules, and forms a charge area by dehydrogenating the hydroxyl groups on the fiber, which attracts small molecule nutrients such as fatty acids, amino acids and polypeptides containing carboxyl and amino groups under the action of electrostatics, thereby stabilizing the active ingredients and preventing them from being converted into toxic substances. The present invention provides a fermented feed composition prepared by the above preparation method, comprising the following components in parts by weight: 11-18 parts of corn, 3-9 parts of straw, 8-13 parts of sunflower seeds, 2-8 parts of wheat bran, 6-15 parts of coconut shells, 5-9 parts of algae mud, and 3-8 parts of fermented protein.

[0006] The invention also provides a fermented feed composition for use in improving the immune function of Qingyuan chickens.

[0007] Beneficial effects: The present invention provides a method for preparing a fermented feed composition for improving the immunity of Qingyuan chickens, wherein the mixture is first decomposed by mixing grain particles and algae mud to obtain a mixture, and then mixed with fermented protein and fermented to obtain a fermented feed, wherein the fermented protein is used as a fermentation base, the protein carrying the strain can react with organic salts through electrostatic action to form a polymer system to stably protect the strain, and the decomposed substances can be efficiently fermented through a secondary fermentation process, and finally enrichment treatment is performed to further eliminate antibiotics and solidify active substances, thereby improving the inhibition of toxins on the immunity of Qingyuan chickens and improving immune function, thereby preparing a fermented feed composition; The fermented feed composition provided by the present invention is prepared by selecting common raw materials and adding fermented protein to obtain a high-protein, low-resistance, high-nutrient fermented feed composition, which is rich in vitamins, probiotics, water-soluble fiber, lactic acid, polypeptides, low-molecular-weight proteins and other elements, has high active ingredients, can retain nutrients and release them slowly in the intestine, can enhance the antioxidant capacity and body immunity of Qingyuan chickens, has good palatability, can regulate the intestinal health of Qingyuan chickens, has easily absorbed nutrients, produces less fixed carbon, does not require the addition of preservatives, can effectively inhibit bacterial growth, and can be stably and long-term stored; The fermented feed composition provided by the present invention is used in feeding Qingyuan chickens, which can reduce and replace the use of antibiotics, comprehensively improve the immunity and anti-irritation ability of Qingyuan chickens, thereby increasing the market rate of Qingyuan chickens and reducing the disease incidence rate of Qingyuan chickens, effectively improving the breeding efficiency of Qingyuan chickens, and is beneficial to the digital breeding of Qingyuan chickens. The Qingyuan chickens obtained by feeding have a low fat content, contain more unsaturated fatty acids and high-density lipoprotein cholesterol, and are healthy and delicious. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] Figure 1 The content change of the components in the fermentation process of the fermented protein of Example 1 of the present invention; Figure 2 It is the content of bacterial colonies in different fermentation periods of Example 1 of the present invention. DETAILED DESCRIPTION

[0009] The present invention is further described in detail below through specific implementation cases. It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of protection of the present invention. After reading the present invention, various equivalent forms of modifications to the present invention by those skilled in the art are all within the scope of the claims attached to this application.

[0010] Unless otherwise specified, all raw materials and reagents of the present invention are raw materials and reagents from the conventional market.

[0011] Example 1 A method for preparing a fermented feed composition for improving the immunity of Qingyuan chickens comprises the following steps: S1: 7 parts of chlorella, giant kelp, brown algae and spirulina are mixed and ground into mud in a mass ratio of 11:6:1:1, and then mixed and added with 6wt% and 13wt% of sodium humate and sodium alginate respectively to prepare algae mud, and then mixed with 15 parts of corn, 6 parts of straw, 11 parts of sunflower seeds, 5 parts of wheat bran and 10 parts of coconut shells to grind into grain particles with a particle size of 2 mm to obtain a mixture; S2: 6 parts of protein raw materials consisting of soybean meal, hemp leaf bamboo shoot processing byproducts and palm meal in a mass ratio of 1:7:3 were mixed, freeze-dried at a temperature of -15°C, extruded and puffed, mixed with a fermentation bacterial suspension at a solid-liquid ratio of 3:12 g / mL, and then mixed with 5wt% of calcium dihydrogen phosphate. The fermented protein was obtained by closed fermentation at a temperature of 40°C for 5 hours, and then mixed with the mixture for secondary fermentation. The secondary fermentation was first performed by ventilation fermentation, and then closed fermentation was performed at a temperature of 35°C in a fermenter for 7 days to obtain a fermented material; the conditions for ventilation fermentation were: 32°C, 250rpm stirring, continuous ventilation at a ventilation ratio of 0.4vvm, and culture for 22h; the fermentation bacterial suspension had a concentration of 1×10 7 CFU / g of Lactobacillus johnsonii BS15, 1×10 5 CFU / g of Lactobacillus casei, 1×10 5 CFU / g of Enterococcus faecalis and 1×10 8 CFU / g of Clostridium butyricum was mixed in PBS buffer containing 13wt% earthworm peptide; the content of the fermented protein components was detected as follows Figure 1 As shown, it can be seen that the fermented feed composition prepared by the present invention contains a variety of small molecules and high-quality proteins, and has excellent immune properties; the activity of the probiotics in the fermented feed composition is tested, and the results are as follows Figure 2 As shown by Figure 2 It can be seen that the bacterial flora used in the present invention can synergistically promote growth and fermentation through the fermentation process; S3: The fermented material is first subjected to ultrasonic treatment at an ultrasonic frequency of 25 KHz for 15 minutes, then subjected to electrostatic treatment at a field strength of 0.6 V / m for 14 minutes for enrichment treatment, and then dried at low temperature under vacuum conditions to obtain a fermented feed composition.

[0012] Example 2 S1: 5 parts of chlorella, giant kelp, brown algae and spirulina are mixed and ground into mud in a mass ratio of 9:4:1:1, and then mixed with sodium humate and sodium alginate in an amount of 4wt% and 10wt% respectively to prepare algae mud, and 11 parts of corn, 3 parts of straw, 8 parts of sunflower seeds, 2 parts of wheat bran and 6 parts of coconut shells are mixed and crushed into grain particles with a particle size of 1 mm to obtain a mixture; S2: 3 parts of protein raw materials consisting of soybean meal, hemp leaf bamboo shoot processing byproducts and palm meal in a mass ratio of 1:5:3 were mixed, freeze-dried at a temperature of -15°C, extruded and puffed, mixed with a fermentation bacterial suspension at a solid-liquid ratio of 3:11 g / mL, and then mixed with 3wt% of calcium dihydrogen phosphate for closed fermentation at a temperature of 30°C for 3h to obtain a fermented protein, which was mixed with the mixture and then fermented twice. The secondary fermentation was first performed by ventilation fermentation, and then the temperature in the fermenter was 30°C for closed fermentation for 6 days to obtain a fermented material; the conditions for ventilation fermentation were: 30°C, 200rpm stirring, continuous ventilation at a ventilation ratio of 0.3vvm, and culture for 20h; the fermentation bacterial suspension had a concentration of 1×10 5 CFU / g of Lactobacillus johnsonii BS15, 1×10 2 CFU / g of Lactobacillus casei, 1×10 5 CFU / g of Enterococcus faecalis and 1×10 8 CFU / g of Clostridium butyricum was mixed in PBS buffer containing 10wt% earthworm peptide.

[0013] S3: The fermented material is first subjected to ultrasonic treatment at an ultrasonic frequency of 20 KHz for 10 minutes, then subjected to electrostatic treatment at a field strength of 0.5 V / m for 11 minutes for enrichment treatment, and then dried at low temperature under vacuum conditions to obtain a fermented feed composition.

[0014] Example 3 S1: 9 parts of chlorella, giant kelp, brown algae and spirulina are mixed and ground into mud in a mass ratio of 13:8:1:1, and then mixed and added with 8wt% and 16wt% of sodium humate and sodium alginate respectively to prepare algae mud, and then mixed with 18 parts of corn, 9 parts of straw, 13 parts of sunflower seeds, 8 parts of wheat bran and 15 parts of coconut shells to grind into grain particles with a particle size of 3 mm to obtain a mixture; S2: 8 parts of protein raw materials consisting of soybean meal, hemp leaf bamboo shoot processing byproducts and palm meal in a mass ratio of 1:9:3 were mixed, freeze-dried at a temperature of -15°C, extruded and puffed, mixed with a fermentation bacterial suspension at a solid-liquid ratio of 3:13 g / mL, and then mixed with 6wt% of calcium dihydrogen phosphate for closed fermentation at a temperature of 50°C for 6 hours to obtain a fermented protein, which was mixed with the mixture and then fermented twice, wherein the secondary fermentation was first performed by ventilation fermentation, and then the temperature in the fermenter was 40°C for 9 days to obtain a fermented material; the conditions for ventilation fermentation were: 34°C, 300rpm stirring, continuous ventilation at a ventilation ratio of 0.5vvm, and cultured for 24h; the fermentation bacterial suspension had a concentration of 1×10 8 CFU / g of Lactobacillus johnsonii BS15, 1×10 7 CFU / g of Lactobacillus casei, 1×10 5 CFU / g of Enterococcus faecalis and 1×10 8CFU / g of Clostridium butyricum was mixed in PBS buffer containing 16wt% earthworm peptide.

[0015] S3: The fermented material is first subjected to ultrasonic treatment at an ultrasonic frequency of 30 KHz for 20 minutes, then subjected to electrostatic treatment at a field strength of 0.8 V / m for 18 minutes for enrichment treatment, and then dried at low temperature under vacuum conditions to obtain a fermented feed composition.

[0016] Example 4 S1: 6 parts of chlorella, giant kelp, brown algae and spirulina are mixed and ground into mud in a mass ratio of 10:5:1:1, and then mixed and added with 5wt% and 12wt% of sodium humate and sodium alginate respectively to prepare algae mud, and then mixed with 13 parts of corn, 4 parts of straw, 9 parts of sunflower seeds, 3 parts of wheat bran and 8 parts of coconut shells to grind into grain particles with a particle size of 1 mm to obtain a mixture; S2: 4 portions of protein raw materials consisting of soybean meal, hemp leaf shoot processing byproducts and palm meal in a mass ratio of 1:6:3 were mixed, freeze-dried at a temperature of -15°C, extruded and puffed, mixed with a fermentation bacterial suspension at a solid-liquid ratio of 3:12 g / mL, and then mixed with 4wt% of calcium dihydrogen phosphate for closed fermentation at a temperature of 35°C for 4 hours to obtain a fermented protein, which was mixed with the mixture and then fermented twice, wherein the secondary fermentation was first performed by ventilation fermentation, and then the temperature in the fermenter was 35°C for closed fermentation for 6 days to obtain a fermented material; the conditions for ventilation fermentation were: 32°C, 220rpm stirring, continuous ventilation at a ventilation ratio of 0.4vvm, and cultured for 20 hours; the fermentation bacterial suspension had a concentration of 1×10 6 CFU / g of Lactobacillus johnsonii BS15, 1×10 3 CFU / g of Lactobacillus casei, 1×10 5 CFU / g of Enterococcus faecalis and 1×10 8 CFU / g of Clostridium butyricum was mixed in PBS buffer containing 12wt% earthworm peptide.

[0017] S3: The fermented material is first subjected to ultrasonic treatment at an ultrasonic frequency of 22 KHz for 12 minutes, then subjected to electrostatic treatment at a field strength of 0.6 V / m for 12 minutes for enrichment treatment, and then dried at low temperature under vacuum conditions to obtain a fermented feed composition.

[0018] Example 5 S1: 8 parts of chlorella, giant kelp, brown algae and spirulina are mixed and ground into mud in a mass ratio of 12:7:1:1, and then mixed and added with 7wt% and 15wt% of sodium humate and sodium alginate respectively to prepare algae mud, and then mixed with 17 parts of corn, 8 parts of straw, 12 parts of sunflower seeds, 7 parts of wheat bran and 14 parts of coconut shells to grind into grain particles with a particle size of 3 mm to obtain a mixture; S2: 7 parts of protein raw materials consisting of soybean meal, hemp leaf bamboo shoot processing byproducts and palm meal in a mass ratio of 1:8:3 were mixed, freeze-dried at a temperature of -15°C, extruded and puffed, mixed with a fermentation bacterial suspension at a solid-liquid ratio of 3:11 g / mL, and then mixed with 5wt% of calcium dihydrogen phosphate and fermented in a closed manner at a temperature of 45°C for 5 hours to obtain a fermented protein, which was mixed with the mixture and then fermented twice. The secondary fermentation was first performed by ventilation fermentation, and then fermented in a closed manner at a temperature of 32°C in a fermenter for 8 days to obtain a fermented material; the conditions for ventilation fermentation were: 33°C, 200rpm stirring, continuous ventilation at a ventilation ratio of 0.5vvm, and cultured for 20 hours; the fermentation bacterial suspension had a concentration of 1×10 7 CFU / g of Lactobacillus johnsonii BS15, 1×10 2 CFU / g of Lactobacillus casei, 1×10 5 CFU / g of Enterococcus faecalis and 1×10 8 CFU / g of Clostridium butyricum was mixed in PBS buffer containing 10wt% earthworm peptide.

[0019] S3: The fermented material is first subjected to ultrasonic treatment at an ultrasonic frequency of 28 KHz for 18 minutes, then subjected to electrostatic treatment at a field strength of 0.8 V / m for 15 minutes for enrichment treatment, and then dried at low temperature under vacuum conditions to obtain a fermented feed composition.

[0020] Example 6 S1: 5 parts of chlorella, giant kelp, brown algae and spirulina are mixed and ground into mud in a mass ratio of 13:4:1:1, and then mixed and added with 8wt% and 10wt% of sodium humate and sodium alginate respectively to prepare algae mud, and then mixed with 18 parts of corn, 3 parts of straw, 13 parts of sunflower seeds, 2 parts of wheat bran and 15 parts of coconut shells to grind into grain particles with a particle size of 1 mm to obtain a mixture; S2: 8 portions of protein raw materials consisting of soybean meal, hemp leaf bamboo shoot processing byproducts and palm meal in a mass ratio of 1:5:3 were mixed, freeze-dried at a temperature of -15°C, extruded and puffed, mixed with a fermentation bacterial suspension at a solid-liquid ratio of 3:13 g / mL, and then mixed with 3wt% of calcium dihydrogen phosphate for closed fermentation at a temperature of 50°C for 3 hours to obtain a fermented protein, which was mixed with the mixture and then fermented twice, wherein the secondary fermentation was first performed by ventilation fermentation, and then fermented in a fermenter at a temperature of 40°C for 6 days to obtain a fermented material; the conditions for ventilation fermentation were: 34°C, 200rpm stirring, continuous ventilation at a ventilation ratio of 0.5vvm, and cultured for 20 hours; the fermentation bacterial suspension had a concentration of 1×10 5 CFU / g of Lactobacillus johnsonii BS15, 1×10 7 CFU / g of Lactobacillus casei, 1×10 5 CFU / g of Enterococcus faecalis and 1×10 8CFU / g of Clostridium butyricum was mixed in PBS buffer containing 10-16wt% earthworm peptide.

[0021] S3: The fermented material is first subjected to ultrasonic treatment at an ultrasonic frequency of 30 KHz for 10 minutes, then subjected to electrostatic treatment at a field strength of 0.5 V / m for 18 minutes for enrichment treatment, and then dried at low temperature under vacuum conditions to obtain a fermented feed composition.

[0022] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that in Comparative Example 1, no fermentation protein is added, and the mixture of S1 is directly mixed with the fermentation bacterial suspension at a solid-liquid ratio of 3:12 g / mL and then mixed with 5 wt% of monocalcium phosphate, and then ventilated fermentation and closed fermentation are carried out under the same conditions, and the other conditions remain unchanged.

[0023] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that no algae mud is added in Comparative Example 2, and the other conditions remain unchanged.

[0024] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that in the fermentation of Comparative Example 3, no secondary fermentation is performed, only closed fermentation is performed, and the closed fermentation conditions are the same, and the other conditions remain unchanged.

[0025] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that no enrichment treatment is performed in Comparative Example 4, and the other conditions remain unchanged.

[0026] Experiment 1: The types and contents of nutrients contained in the fermented feed composition prepared in Example 1 and the common complete feed purchased on the market (control group) are shown in Table 1; Test 1: Nutritional component testing Table 1 Nutritional composition of fermented feed composition Nutritional categories unit Example 1 Control group Amino Acids % 4.77 1.96 Crude Protein % 18.46 33.71 Crude fat % 8.61 3.55 fatty acid % 13.67 3.64 Crude Fiber % 14.33 8.25 Soluble Fiber % 18.34 4.95 Polysaccharides % 6.72 0.35 lactic acid % 6.25 1.45 Vitamins % 8.53 3.46 Probiotics % 3.67% 0 Ash % 4.19 9.38 It can be seen from Table 1 that the fermented feed composition prepared by the present invention has rich and high-content nutrients and has good nutritional value.

[0027] Test 2: Immune function test 10-day-old healthy young chickens were selected and divided into 10 groups, each with 20 chickens, and fed with the feed prepared in the embodiment and the comparative example, respectively. Ordinary chicken feed purchased on the market was set as the control group, and fed to adult chickens. The feeding period was 50 days, the number of feedings per day was 3 times, and the body weight before and after feeding was recorded. The number of chickens marketed and the weight of the chickens were recorded to calculate the market rate and weight growth rate. The results are shown in Table 2. The immune indicators of the Qingyuan chickens fed are shown in Table 3. The body properties of the Qingyuan chickens in Example 1 and the control group are shown in Table 4, and the antioxidant properties are shown in Table 5.

[0028] Table 2 Feeding results of Qingyuan chicken Test groups Number of slaughtered Weight gain Example 1 50 258% Example 2 50 247% Example 3 50 351% Example 4 50 255% Example 5 50 243% Example 6 50 240% Comparative Example 1 45 216% Comparative Example 2 40 205% Comparative Example 3 43 209% Control group 37 170% It can be seen from Table 2 that the fermented feed composition prepared by the present invention can reduce the morbidity and mortality of Qingyuan chickens, and the growth performance of Qingyuan chickens is good, and the weight gain is obvious.

[0029] Table 3 Immune index of Qingyuan chicken Test groups Thymus index (g / kg) Spleen index (g / kg) Bursal index (g / kg) Serum IgA (ng / mL) Serum IgM (ng / mL) Serum IgY (ug / mL) Example 1 1.82±0.43 1.33±0.48 1.39±0.38* 998.25±4.33* 701.46±1.91* 23.67±0.88* Example 2 1.78±0.51 1.32±0.35 1.36±0.43* 993.35±3.27* 696.34±1.77* 22.99±0.72* Example 3 1.81±0.54 1.30±0.21 1.33±0.27* 990.48±5.69* 698.74±2.12* 21.76±0.64* Example 4 1.79±0.34 1.29±0.46 1.32±0.25* 989.90±6.15* 695.97±2.01* 23.01±0.74* Example 5 1.76±0.31 1.32±0.28 1.30±0.22* 994.81±3.94* 697.31±2.24* 21.58±0.81* Example 6 1.80±0.49 1.32±0.30 1.34±0.28* 991.47±2.97* 694.31±2.39* 21.93±0.50* Comparative Example 1 1.50±0.36 1.15±0.34 1.21±0.26 930.34±5.97* 637.64±2.57* 18.64±0.35* Comparative Example 2 1.49±0.50 1.17±0.41 1.20±0.21 919.66±4.74* 629.74±3.01* 19.63±0.21* Comparative Example 3 1.52±0.47 1.14±0.29 1.23±0.24 931.12±3.45* 633.79±2.94* 17.26±0.79* Comparative Example 4 1.45±0.36 1.10±0.22 1.12±0.28 915.38±3.05* 621.48±2.59* 15.15±0.31* Control group 1.60±0.67 1.15±0.50 1.11±0.27 785.67±3.24 591.29±2.83 15.88±0.71 Note: * indicates significant difference between the control group and the experimental group (P<0.05) As shown in Table 3, the immunoglobulin concentration in the serum of the Qingyuan chickens fed with the chickens according to the embodiment of the present invention was significantly increased, and the immune organ index was also increased to a certain extent.

[0030] Table 4 Physical functions of Qingyuan chicken Test groups Chest muscle rate (%) Leg muscle rate (%) Abdominal fat percentage (%) Pectoral muscle drip loss (%) Example 1 19.25 23.64 5.08 998.25±4.33* Control group 15.36 19.26 7.67 785.67±3.24 It can be seen from Table 4 that the muscle quality of the Qingyuan chicken prepared by the present invention is high, indicating that the fermented feed composition prepared by the present application can effectively improve the body performance of the Qingyuan chicken.

[0031] Table 5 Antioxidant properties of Qingyuan chicken Test groups Superoxide dismutase activity (U / mL) Serum malondialdehyde content (U / mL) Glutathione peroxidase activity (nmol / mL) Example 1 167.17±4.97 6.02±0.57 2294.66±36.99 Control group 113.58±6.47 6.93±0.19 2011.37±28.47 As shown in Table 5, compared with the control group, the superoxide dismutase activity and glutathione peroxidase activity of the embodiment were both improved, and the serum malondialdehyde content was reduced, indicating that the fermented feed composition prepared by the present invention also improved the antioxidant performance of Qingyuan chicken.

[0032] Test 3: Toxin Residue The fermented feed composition of Example 1 and ordinary chicken feed purchased on the market that meets the qualified standards were fed to two chicken groups, each group had 20 chickens of the same growth period with the same health status. The feeding period was one month, and the toxin residues in the blood and liver of each chicken were tested respectively. The test results of the average toxin residues in each chicken are shown in Table 6.

[0033] Table 6 Aflatoxin residues in blood and liver Test groups Residue in blood (ng / g) Residue in liver (ng / g) Example 1 0.007±0.0012 0.0005±0.0001 Comparative Example 3 5.64±0.13 4.38±0.21 Comparative Example 4 6.32±0.09 6.97±0.15 Control group 18.35±0.20 11.71±0.34 As can be seen from Table 6, the fermented feed composition prepared in Example 1 of the present invention has less aflatoxin residues in the blood and liver of chickens fed with general feed, while the toxin residues in Comparative Examples 3-4 are relatively high, and the toxin detection amount of the feed purchased on the market is the highest, indicating that the fermented feed composition prepared in the present invention can eliminate toxins and improve immunity.

[0034] In summary, the fermented feed composition prepared by the present application enriches the content of immune active substances by first fermenting and then fermenting protein for a second time, and eliminates toxins, which can effectively improve the immune function and body function of Qingyuan chickens. The Qingyuan chickens obtained by feeding have good ketone body function, high resistance, and low mortality. The fermented feed composition prepared by the present invention is suitable for large-scale breeding of Qingyuan chickens.

Claims

1. A method for preparing a fermented feed composition for improving the immunity of Qingyuan chickens, characterized in that: The following steps are involved: S1: mixing chlorella, giant kelp, brown algae and spirulina in a mass ratio of 9-13:4-8:1:1, grinding them into mud, mixing them with sodium humate and sodium alginate to prepare algae mud, and mixing them with cereal particles to obtain a mixed material; The cereal particles are obtained by mixing corn, straw, sunflower seeds, wheat bran and coconut shells and grinding them into particles with a diameter of 1-3 mm; S2: mixing the mixed material with the fermented protein and then performing secondary fermentation, wherein the secondary fermentation is firstly performed with ventilation fermentation, and then performed closed fermentation in a fermentation tank at a temperature of 30-40° C. for 6-9 days to obtain a fermented material; The fermented protein is prepared by mixing protein raw materials consisting of soybean meal, hemp leaf bamboo shoot processing byproducts and palm meal in a mass ratio of 1:5-9:3, freeze-drying, extruding and puffing, and then fermenting a mixed fermentation bacterial suspension in a solid-liquid ratio of 3:11-13 g / mL; S3: enriching the fermented material, and then drying it at low temperature under vacuum conditions to obtain a fermented feed composition.

2. The method for preparing a fermented feed composition for improving immunity of Qingyuan chicken according to claim 1, characterized in that: The addition amounts of sodium humate and sodium alginate in S1 are 4-8wt% and 10-16wt% respectively.

3. The method for preparing a fermented feed composition for improving immunity of Qingyuan chicken according to claim 1, characterized in that: The conditions of the aeration fermentation in S2 are: 25-35° C., stirring at 150-180 rpm, continuous ventilation at an aeration ratio of 0.4-0.7 vvm, and cultivation for 15-18 hours.

4. The method for preparing a fermented feed composition for improving immunity of Qingyuan chicken according to claim 1, characterized in that: The fermentation bacterial suspension in S2 has a concentration of 1×10 5 ~1×10 8 CFU / g of Lactobacillus johnsonii BS15, 1×10 2 ~1×10 7 CFU / g of Lactobacillus casei, 1×10 5 CFU / g of Enterococcus faecalis and 1×10 8 CFU / g of Clostridium butyricum was mixed in the hydrolyzate.

5. The method for preparing a fermented feed composition for improving immunity of Qingyuan chicken according to claim 4, characterized in that: The hydrolyzate is a PBS buffer solution containing 10-16 wt % of earthworm peptide.

6. The method for preparing a fermented feed composition for improving immunity of Qingyuan chicken according to claim 1, characterized in that: S2 The fermentation of the fermented protein is to mix the protein raw material with 3-6wt% of monocalcium phosphate and ferment it in a closed manner at a temperature of 30-50°C for 3-6 hours.

7. The method for preparing a fermented feed composition for improving immunity of Qingyuan chicken according to claim 1, characterized in that: The enrichment treatment in S3 is to first subject the fermented material to ultrasonic treatment at an ultrasonic frequency of 20-30 KHz for 10-20 minutes and then to ultrasonic electrostatic treatment at a field strength of 0.5-0.8 V / m for 11-18 minutes.

8. The fermented feed composition prepared by the preparation method according to any one of claims 1 to 7, characterized in that: The invention comprises the following components in parts by weight: 11-18 parts of corn, 3-9 parts of straw, 8-13 parts of sunflower seeds, 2-8 parts of wheat bran, 6-15 parts of coconut shell, 5-9 parts of algae mud and 3-8 parts of fermented protein.

9. Use of the fermented feed composition according to any one of claim 8 in improving the immune function of Qingyuan chickens.

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