Compound bacteria fermented compound traditional Chinese medicine scalp care composition and application thereof

Abstract

The invention discloses a compound bacteria fermentation compound traditional Chinese medicine scalp care composition and application thereof, and belongs to the technical field of daily necessities for washing and caring, and a preparation method of the compound bacteria fermentation compound traditional Chinese medicine scalp care composition comprises the following steps: preparing a fermentation culture medium, preparing a lactobacillus solution, preparing a saccharomycetes solution, preparing a mixed bacteria solution, fermenting and mixing; in the step of material mixing, fermentation liquor, sodium hyaluronate, glycerin, phenoxyethanol, ethylhexylglycerin, butanediol, a stabilizer, dodecyl betaine and deionized water are mixed and then homogenized, and the compound bacteria fermentation compound traditional Chinese medicine scalp care composition is obtained. The prepared compound bacteria fermentation compound traditional Chinese medicine scalp care composition is good in storage resistance, loss of important volatile substances can be reduced in the preparation process, and therefore the rich natural active ingredients in the traditional Chinese medicine can play a role to the maximum extent, and further, the scalp care effect is improved. The shampoo can promote growth of scalp hair follicles, inhibit scalp inflammation, increase hair glossiness, increase permeability of active ingredients and improve the anti-alopecia effect.

Description

Technical Field

[0001] The invention relates to the technical field of daily washing and care products, and in particular to a composite bacterial fermentation compound traditional Chinese medicine scalp care composition and application thereof. Background Art

[0002] With the influence of the current living environment, the increase of life pressure, sub-health and other conditions, hair problems are also increasing, and it is easy to have dry and frizzy hair, excessive scalp oil secretion, dandruff, scalp sensitivity and other problems. The World Health Organization has also listed "glossy hair and no dandruff" as one of the standards of human health. As consumers pay more and more attention to scalp care, various functional products are constantly developing and gradually refined, from the initial oil control, dandruff removal, care, moisturizing and other functions to black hair, anti-hair loss, hair growth and other functions.

[0003] For scalp and hair care products with repairing effects, the original washing and care products are mainly composed of chemical synthetic substances, but long-term use will cause various side effects, such as dry and yellow hair, hair breakage, hair loss, etc. Therefore, more and more manufacturers have begun to produce plant care products, and solve the side effects caused by chemical synthetic substances by adding a variety of herbs, but the care effect is not significant. According to research, the plant ingredients in existing plant care products are mainly obtained by conventionally crushing Chinese medicinal materials, and the large amount of macromolecular or polymer components such as proteins contained therein are difficult to obtain good absorption, thereby hindering the penetration effect of active substances. Nowadays, some care products also increase the content of effective ingredients in Chinese medicine by microbial fermentation of Chinese medicine, but there are the following problems in the fermentation process of Chinese medicine: first, the fermented Chinese medicine is more susceptible to microbial contamination, resulting in poor storage resistance; second, Chinese medicine contains volatile substances, which will cause a large amount of volatile substances to be lost during the fermentation process, affecting the care effect. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a composite bacterial fermentation compound Chinese medicine scalp care composition and application thereof. The composite bacterial fermentation compound Chinese medicine scalp care composition of the present invention has good storage stability, can reduce the loss of important volatile substances during preparation, thereby maximizing the effect of the rich natural active ingredients in the Chinese medicine, and further, can promote the growth of scalp hair follicles, inhibit scalp inflammation, increase hair gloss, increase the permeability of active ingredients, and can also improve the anti-hair loss effect.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows: A composite bacterial fermentation compound traditional Chinese medicine scalp care composition, the preparation method of which comprises the following steps: preparing a fermentation medium, preparing a lactobacillus liquid, preparing a yeast liquid, preparing a mixed liquid, fermenting, and mixing; The method comprises the following steps of: selecting fresh, pest-free Rehmannia root, Ligustrum lucidum fruit, Polygonatum sibiricum, Platycladus orientalis leaf and Chuanxiongqiong, cleaning them respectively and crushing them into 120-150 meshes to obtain Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder and Chuanxiongqiong powder; then uniformly mixing the Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder and Chuanxiongqiong powder to obtain mixed Chinese medicine powder; mixing the mixed Chinese medicine powder, anhydrous ethanol and deionized water, soaking them at room temperature for 3-4 hours, performing ultrasonic reflux extraction, and controlling the mixture to obtain a mixed Chinese medicine powder; and The temperature of ultrasonic reflux extraction is 65-70°C, the ultrasonic power is 90-120W, the time is 1-1.5h, and then filtering is performed, taking the filtrate, adding cross-linked microspheres to the filtrate to obtain a mixed solution, and then concentrating the mixed solution to 20-25% of the original volume by a rotary evaporator, and then adjusting the pH to 6-7 to obtain a concentrated solution, and after the concentrated solution is evenly mixed with glucose, sterilized at 121°C for 20-30min, and naturally cooled to room temperature to obtain a fermentation medium; In the preparation of the fermentation medium, the mass ratio of Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder and Chuanxiong powder is 20-30:10-25:10-20:20-40:8-20; The mass volume ratio of mixed Chinese medicine powder, anhydrous ethanol and deionized water is 100g:750-800mL:80-120mL; The mass volume ratio of the filtrate to the cross-linked microspheres is 800-850 mL:7-8 g; The mass volume ratio of the concentrate to glucose is 100mL:2.5-3.5g; The preparation method of the cross-linked microspheres comprises the following steps: cross-linking and mixing; The cross-linking is performed by uniformly mixing sodium hyaluronate and sodium hydroxide aqueous solution to obtain sodium hyaluronate gel; uniformly mixing Tween 80 and liquid paraffin to obtain an oil phase; while stirring the oil phase at a stirring speed of 400-500 rpm, dripping the water phase into the oil phase, and after the dripping is completed, transferring the mixture to a high-pressure homogenizer for homogenization, controlling the pressure of the high-pressure homogenizer to be 120-150 MPa, and homogenizing for 3-4 times to obtain a homogenized liquid; mixing the homogenized liquid with 1,4-butanediol diglycidyl After mixing with ether, stir at a stirring speed of 300-500 rpm for 4-5 hours, let stand for 13-15 hours, transfer to a centrifuge for centrifugation, control the speed of the centrifuge to 10000-11000 rpm, the time is 9-10 minutes, take the precipitate, wash the precipitate with ethyl acetate 3-4 times, wash the precipitate with anhydrous ethanol 3-4 times, transfer to an oven for drying, control the temperature of the oven to 60-70° C., the time is 14-16 hours, and obtain sodium hyaluronate microspheres; In the cross-linking, the molecular weight of the sodium hyaluronate is 1.5 million to 2.5 million Da; The mass volume ratio of sodium hyaluronate and sodium hydroxide aqueous solution is 10g:50-100mL; The mass concentration of the sodium hydroxide aqueous solution is 2%; The mass volume ratio of Tween 80 and liquid paraffin is 7g:370-400mL; The volume ratio of oil phase to water phase is 380-400:510-560; The water phase is added for 40-50 min. The volume ratio of the homogenized liquid to 1,4-butanediol diglycidyl ether is 900-950:7-8; The mixing is performed, adding kaolin and sodium hyaluronate into a ball mill for ball milling, controlling the ball-to-material ratio of the ball mill to be 10-12:1, the rotation speed to be 350-400rpm, and ball milling for 1-1.5h to obtain modified kaolin; after the modified kaolin, sodium hyaluronate microspheres and deionized water are mixed, stirring at a stirring speed of 200-300rpm for 2-3h, then transferring to a centrifuge for centrifugation, controlling the rotation speed of the centrifuge to be 8000-9000rpm, the time to be 9-10min, taking the precipitate, washing it with deionized water for 3-4 times, transferring to an oven for drying, controlling the temperature of the oven to be 60-80°C, the time to be 28-30h, and obtaining cross-linked microspheres; In the mixture, the mass ratio of kaolin to sodium hyaluronate is 30:10-12; The mass volume ratio of modified kaolin, sodium hyaluronate microspheres, and deionized water is 20-22 g: 35-40 g: 900-1000 mL; The molecular weight of the sodium hyaluronate is 200,000 Da; The lactobacillus culture liquid is prepared by inoculating the lactobacillus into an MRS culture medium for single strain enrichment culture, culturing the culture at 35-37° C. for 40-48 hours to obtain a primary seed liquid, and then inoculating the primary seed liquid into an MRS culture medium at an inoculation amount of 2.5-3% (volume fraction), culturing the culture at 35-37° C. for 20-25 hours to obtain a secondary seed liquid, i.e., the lactobacillus culture liquid; The preparation method of the MRS culture medium comprises the following steps: evenly mixing peptone, beef extract, yeast extract, diammonium citrate, glucose, sodium acetate, magnesium sulfate, manganese sulfate and distilled water, adjusting the pH to 6.2-6.6, and sterilizing at 121° C. for 20-30 minutes to obtain the MRS culture medium; In the preparation of the MRS medium, the mass volume ratio of peptone, beef extract, yeast extract, diammonium citrate, glucose, sodium acetate, magnesium sulfate, manganese sulfate, and distilled water is 10-12 g: 5-6 g: 4-4.5 g: 2-2.1 g: 20-25 g: 5-6 g: 0.2-0.25 g: 0.05-0.06: 100-130 mL; The lactobacillus liquid contains 1×10 9 -7×10 9 CFU / mL; The yeast liquid is prepared by inoculating the yeast into a YPD medium for single strain enrichment culture, culturing the medium at 28-30° C. for 20-24 hours to obtain a first-level seed liquid, and then inoculating the first-level seed liquid into a YPD medium at an inoculation rate of 2.5-3% (volume fraction), culturing the medium at 28-30° C. for 35-36 hours to obtain a second-level seed liquid, i.e., a yeast liquid; The preparation method of the YDP medium is as follows: yeast extract, peptone, glucose and distilled water are uniformly mixed, the pH is adjusted to 6.2-6.6, and sterilized at 121° C. for 20-30 minutes to obtain the YDP medium; In the YDP medium, the mass volume ratio of yeast extract, peptone, glucose and distilled water is 10-12g:20-22g:20-23g:100-120mL; The yeast liquid has a bacterial content of 6×10 8 -1×10 9 CFU / mL; The mixed bacterial solution is prepared by uniformly mixing the lactobacillus bacterial solution and the yeast bacterial solution in a volume ratio of 1:1-1.5 to obtain a mixed bacterial solution; The fermentation comprises inoculating the mixed bacterial liquid into a fermentation medium at an inoculation rate of 2-3% (volume fraction), and fermenting at 36-38° C. for 60-70 hours to obtain a fermentation liquid; The mixture is mixed with the fermentation liquid, sodium hyaluronate, glycerin, phenoxyethanol, ethylhexylglycerin, butylene glycol, stabilizer, lauryl betaine, and deionized water, and then transferred into a high-pressure homogenizer for homogenization, the pressure of the high-pressure homogenizer is controlled to be 120-150 MPa, and the homogenization times are 3-4 times to obtain a composite bacteria fermentation compound Chinese medicine scalp care composition; In the mixture, the mass ratio of fermentation liquid, sodium hyaluronate, glycerin, phenoxyethanol, ethylhexylglycerin, butylene glycol, stabilizer, dodecyl betaine and deionized water is 20-30:1.5-2:3-5:0.5-1:0.01-1:4-5:3-3.5:1.5-2:75-80; The molecular weight of the sodium hyaluronate is 100,000 Da; The preparation method of the stabilizer is as follows: betaine, acetylated sodium hyaluronate and kaolin are added into a ball mill for ball milling, the ball-to-material ratio of the ball mill is controlled to be 10-12:1, the rotation speed is 350-400 rpm, and the ball milling is performed for 2-2.5 hours to obtain the stabilizer; In the preparation of the stabilizer, the mass ratio of betaine, acetylated sodium hyaluronate and kaolin is 20-25:12-15:90-100; The acetylated sodium hyaluronate has an acetyl substitution degree of 3.

[0006] An application of the aforementioned composite bacteria fermentation compound traditional Chinese medicine composition for scalp care.

[0007] Compared with the prior art, the present invention has the following beneficial effects: (1) The composite bacterial fermentation compound Chinese medicine scalp care composition of the present invention is prepared by adding cross-linked microspheres to the step of preparing the fermentation medium. Specifically, in the step of preparing the fermentation medium, the cross-linked microspheres are added to the Chinese medicine extract, which is then concentrated. When preparing the cross-linked microspheres, sodium hyaluronate microspheres are first prepared, and then kaolin is intercalated and surface-coated with small molecule sodium hyaluronate to obtain modified kaolin. The modified kaolin is then mixed with the sodium hyaluronate microspheres, and the modified kaolin is adsorbed into the cross-linked sodium hyaluronate microspheres through hydrogen bonds to obtain cross-linked microspheres, thereby further increasing the adsorption capacity of the cross-linked sodium hyaluronate microspheres. The cross-linked microspheres are then added to the Chinese medicine extract. The cross-linked microspheres can adsorb and slowly release volatile substances in the Chinese medicine extract, thereby avoiding the loss of volatile substances and improving the maintenance of the composite bacterial fermentation compound Chinese medicine scalp care composition. The invention discloses a novel method for preparing the scalp care composition of the present invention, wherein the scalp care composition of the present invention has the advantages of good moisturizing effect, good content of Chinese medicine active ingredients, and good hair follicle growth effect. In addition, the cross-linked microspheres can also protect volatile substances, thereby improving the storage resistance of the composite bacterial fermentation composite Chinese medicine scalp care composition; a stabilizer is added to the mixture, and the preparation method of the stabilizer is to mix betaine, acetylated sodium hyaluronate, and kaolin and then ball mill them. During the ball milling process, betaine and acetylated sodium hyaluronate intercalate and surface modify the kaolin. The presence of betaine can promote the dispersion of kaolin while improving the antibacterial ability of kaolin. Compared with sodium hyaluronate, acetylated sodium hyaluronate has a small molecular weight and strong water absorption. After being intercalated in kaolin, it can improve the moisturizing ability of kaolin and avoid the discomfort caused by the use of kaolin. The presence of kaolin can also stabilize, adsorb, and slowly release the active ingredients in the Chinese medicine, thereby improving the storage resistance of the composite bacterial fermentation composite Chinese medicine scalp care composition; (2) Traditional Chinese medicine has a long history of preventing and treating hair loss and is of great significance in traditional medicine. Traditional Chinese medicine believes that the prevention and treatment of hair loss can be divided into five aspects, including: First, insufficient kidney essence: The kidney is responsible for bone and marrow production, and its essence is in the hair. Therefore, insufficient kidney essence can easily lead to hair loss; Second, Yin deficiency and hyperactivity of fire: Yin deficiency leads to hyperactivity of fire, which can easily lead to blood heat and wind dryness, thereby consuming Yin blood and making the hair unable to be nourished, causing hair loss; Third, spleen deficiency: spleen deficiency can easily lead to a decline in the function of transporting and transforming the essence of water and grain, resulting in the hair not being nourished, causing hair loss; Fourth, blood stasis and obstruction of the collaterals: blood stasis and obstruction of the collaterals can lead to qi and blood stagnation, and poor local blood circulation can easily cause hair loss; Fifth, insufficient qi and blood: Hair is the residue of blood. If qi and blood are insufficient, it can easily lead to dry hair or even hair loss. The composite bacterial fermentation compound Chinese medicine scalp care composition of the present invention is mainly composed of deficiency-tonifying, heat-clearing and blood-activating and stasis-removing drugs, among which the representative deficiency-tonifying drugs are polygonatum, rehmannia root, and Ligustrum lucidum fruit; the heat-clearing drug is Platycladus orientalis leaf; and the blood-activating and stasis-removing drug is Chuanxiong. Among them, Rehmannia root is sweet and slightly warm in nature, enters the liver and kidney meridians, contains styrene glycoside components such as verbascoside, and also contains monosaccharides, amino acids, vitamin A substances, and the like. Ligustrum lucidum fruit is sweet, bitter, and cool in nature; enters the liver and kidney meridians, and contains triterpenoid components, iridoid glycoside components, flavonoid components, fatty acid components, volatile oils, polysaccharides, and the like. Platycladus orientalis leaf is bitter, slightly cold, clears and drains, is astringent, sticky, and astringent, and enters the lung, liver, spleen, and large intestine meridians; contains flavonoid components such as quercetin, quercetin, kaempferol, and the like; contains volatile oils such as cedarwood, acetic acid pine oil, and tannins, and the like. Chuanxiong is pungent and warm in nature. It enters the liver, gallbladder, and pericardium meridians, and contains alkaloids, volatile oils, phenolic substances, visfatin, vitamin A, folic acid, sucrose, sterols, fatty oils, and other ingredients. Polygonatum sibiricum is sweet and flat in nature, and enters the spleen, lung, and kidney meridians; it contains polysaccharides, saponins, flavonoids, and other ingredients; (3) The composite bacterial fermentation compound Chinese medicine scalp care composition of the present invention is non-irritating to the skin. Tests on mice showed that there was no allergic reaction such as erythema on the skin surface after use; (4) The composite bacterial fermentation compound Chinese medicine scalp care composition of the present invention can reduce the loss of important volatile substances during preparation, thereby maximizing the effect of the rich natural active ingredients in the Chinese medicine. Furthermore, it can promote the growth of scalp hair follicles, inhibit scalp inflammation, increase hair gloss, increase the permeability of active ingredients, and improve the anti-hair loss effect. The composite bacterial fermentation compound Chinese medicine scalp care composition of the present invention can significantly improve dry and frizzy hair, and the hair follicle hair growth rate can reach 180-220%; (5) The composite bacterial fermentation compound traditional Chinese medicine scalp care composition of the present invention has a total flavonoid content of 0.64-0.70wt%, a total polyphenol content of 11.58-13.40wt%, and a total sugar content of 10.36-12.42wt%; (6) The composite bacteria fermented composite Chinese medicine scalp care composition of the present invention has good storage stability. After being left open at room temperature for 30 days, the total flavonoids content is 0.63-0.65wt%, the total polyphenols content is 11.26-12.87wt%, and the total sugar content is 10.12-12.14wt%. DETAILED DESCRIPTION

[0008] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific implementation methods of the present invention are now described.

[0009] Example 1 A composite bacterial fermentation compound traditional Chinese medicine scalp care composition, the preparation method of which comprises: 1. Prepare fermentation medium: select fresh, disease-free and insect-free Rehmannia root, Ligustrum lucidum, Polygonatum sibiricum, Platycladus orientalis leaf, and Chuanxiong, clean them respectively and crush them into 120 meshes to obtain Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder, and Chuanxiong powder, then mix Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder, and Chuanxiong powder in a mass ratio of 20:10:10:20:8 to obtain mixed Chinese medicine powder; mix the mixed Chinese medicine powder, anhydrous ethanol, and deionized water in a mass volume ratio of 100g:750mL:80mL, soak them at room temperature for 3h, and perform ultrasonic Reflux extraction, control the temperature of ultrasonic reflux extraction to 65°C, the ultrasonic power to 90W, the time to 1h, then filter, take the filtrate, add cross-linked microspheres to the filtrate, control the mass volume ratio of the filtrate to the cross-linked microspheres to 800mL:7g, obtain a mixed solution, and then use a rotary evaporator to concentrate the mixed solution to 20% of the original volume, and then adjust the pH to 6 to obtain a concentrated solution, mix the concentrated solution and glucose at a mass volume ratio of 100mL:2.5g, sterilize at 121°C for 20min, and naturally cool to room temperature to obtain a fermentation medium; The preparation method of the cross-linked microspheres is: (1) Cross-linking: Sodium hyaluronate and a 2% sodium hydroxide aqueous solution were mixed at a mass volume ratio of 10 g:50 mL to obtain a sodium hyaluronate gel; Tween 80 and liquid paraffin were mixed at a mass volume ratio of 7 g:370 mL to obtain an oil phase; while stirring the oil phase at a stirring speed of 400 rpm, the water phase was added dropwise to the oil phase, the volume ratio of the oil phase to the water phase was controlled to be 380:510, the water phase was added dropwise for 40 min, and after the addition was completed, the mixture was transferred to a high-pressure homogenizer for homogenization, and the high-pressure homogenizer was controlled to be homogenized. The pressure is 120 MPa, and the homogenization times are 3 times to obtain a homogenous liquid; the homogenous liquid is mixed with 1,4-butanediol diglycidyl ether at a volume ratio of 900:7, stirred at a stirring speed of 300 rpm for 4 hours, allowed to stand for 13 hours, transferred to a centrifuge for centrifugation, the speed of the centrifuge is controlled to be 10000 rpm, the time is 9 minutes, the precipitate is taken, the precipitate is washed 3 times with ethyl acetate, the precipitate is washed 3 times with anhydrous ethanol, and transferred to an oven for drying, the temperature of the oven is controlled to be 60°C, the time is 14 hours, and sodium hyaluronate microspheres are obtained; The molecular weight of the sodium hyaluronate is 1.5 million Da; (2) Mixing: Add kaolin and sodium hyaluronate in a mass ratio of 30:10 into a ball mill for ball milling, control the ball-to-material ratio of the ball mill to 10:1, and rotate at 350 rpm for 1 h to obtain modified kaolin; mix the modified kaolin, sodium hyaluronate microspheres, and deionized water in a mass-to-volume ratio of 20 g:35 g:900 mL, stir at a stirring speed of 200 rpm for 2 h, and then transfer to a centrifuge for centrifugation at a speed of 8000 rpm for 9 min. Take the precipitate, wash it with deionized water for 3 times, and transfer it to an oven for drying at a temperature of 70°C for 28 h to obtain cross-linked microspheres; The molecular weight of the sodium hyaluronate is 200,000 Da; 2. Preparation of lactobacillus culture liquid: inoculate lactobacillus into MRS medium for single strain enrichment culture, incubate at 35°C for 40 hours to obtain primary seed liquid, then inoculate the primary seed liquid into MRS medium at an inoculum amount of 2.5% (volume fraction), incubate at 35°C for 20 hours to obtain secondary seed liquid, i.e., lactobacillus culture liquid; The preparation method of the MRS culture medium is as follows: 10g of peptone, 5g of beef extract, 4g of yeast extract, 2g of diammonium citrate, 20g of glucose, 5g of sodium acetate, 0.2g of magnesium sulfate, 0.05-0.06g of manganese sulfate, and 100mL of distilled water are mixed evenly, the pH is adjusted to 6.2, and the mixture is sterilized at 121°C for 30min to obtain the MRS culture medium; The lactobacillus liquid contains 1×10 9 CFU / mL; 3. Prepare yeast culture liquid: inoculate yeast into YPD medium for single strain enrichment culture, incubate at 28°C for 20 hours to obtain primary seed liquid, then inoculate the primary seed liquid into YPD medium at an inoculum amount of 2.5% (volume fraction), incubate at 28°C for 35 hours to obtain secondary seed liquid, i.e. yeast culture liquid; The YDP medium is prepared by mixing 10 g yeast extract, 20 g peptone, 20 g glucose, and 100 mL distilled water, adjusting the pH to 6.2, and sterilizing at 121° C. for 30 min to obtain the YDP medium; The yeast liquid has a bacterial content of 6×10 8 CFU / mL; 4. Prepare a mixed bacterial solution: mix the lactobacillus bacterial solution and the yeast bacterial solution in a volume ratio of 1:1 to obtain a mixed bacterial solution; 5. Fermentation: Inoculate the mixed bacterial solution into the fermentation medium at an inoculum rate of 2% (volume fraction), and ferment at 36°C for 60 hours to obtain a fermentation liquid; 6. Mixing: Mix the fermentation liquid, sodium hyaluronate, glycerin, phenoxyethanol, ethylhexylglycerin, butylene glycol, stabilizer, dodecyl betaine and deionized water in a mass ratio of 20:1.5:3:0.5:0.01:4:3:1.5:75 and transfer them into a high-pressure homogenizer for homogenization. The pressure of the high-pressure homogenizer is controlled to be 120 MPa and the homogenization times are 3 times to obtain a composite bacteria fermentation compound Chinese medicine scalp care composition; The molecular weight of the sodium hyaluronate is 100,000 Da; The preparation method of the stabilizer is as follows: betaine, acetylated sodium hyaluronate and kaolin are added into a ball mill in a mass ratio of 20:12:90 for ball milling, the ball-to-material ratio of the ball mill is controlled to be 10:1, the rotation speed is 350 rpm, and the ball milling is performed for 2 hours to obtain the stabilizer; The acetylated sodium hyaluronate has an acetyl substitution degree of 3.

[0010] This embodiment also provides an application of the aforementioned composite bacteria fermentation compound traditional Chinese medicine composition for scalp care.

[0011] Example 2 A composite bacterial fermentation compound traditional Chinese medicine scalp care composition, the preparation method of which comprises: 1. Prepare fermentation medium: select fresh, pest-free Rehmannia root, Ligustrum lucidum, Polygonatum sibiricum, Platycladus orientalis leaf, and Rhizoma Chuanxiong, clean them respectively and crush them into 120 meshes to obtain Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder, and Rhizoma Chuanxiong powder, then mix Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder, and Rhizoma Chuanxiong powder in a mass ratio of 25:20:15:30:15 to obtain mixed Chinese medicine powder; mix the mixed Chinese medicine powder, anhydrous ethanol, and deionized water in a mass volume ratio of 100g:780mL:100mL, soak them at room temperature for 3.5h, and perform ultrasonic Reflux extraction, control the temperature of ultrasonic reflux extraction to 68°C, the ultrasonic power to 100W, the time to 1.5h, then filter, take the filtrate, add cross-linked microspheres to the filtrate, control the mass volume ratio of the filtrate to the cross-linked microspheres to 820mL:7.5g, obtain a mixed solution, and then use a rotary evaporator to concentrate the mixed solution to 23% of the original volume, and then adjust the pH to 6.5 to obtain a concentrated solution, mix the concentrated solution and glucose at a mass volume ratio of 100mL:3g, sterilize at 121°C for 25min, and naturally cool to room temperature to obtain a fermentation medium; The preparation method of the cross-linked microspheres is: (1) Cross-linking: Sodium hyaluronate and a 2% sodium hydroxide aqueous solution were mixed at a mass volume ratio of 10 g:80 mL to obtain a sodium hyaluronate gel; Tween 80 and liquid paraffin were mixed at a mass volume ratio of 7 g:380 mL to obtain an oil phase; while stirring the oil phase at a stirring speed of 450 rpm, the water phase was added dropwise to the oil phase, the volume ratio of the oil phase to the water phase was controlled to be 390:550, the water phase was added dropwise for 45 min, and after the addition was completed, the mixture was transferred to a high-pressure homogenizer for homogenization, and the pressure of the high-pressure homogenizer was controlled. The pressure was 140 MPa, and the homogenization times were 3 times to obtain a homogenous liquid; the homogenous liquid was mixed with 1,4-butanediol diglycidyl ether at a volume ratio of 920:7.5, stirred at a stirring speed of 400 rpm for 4.5 hours, allowed to stand for 14 hours, transferred to a centrifuge for centrifugation, the speed of the centrifuge was controlled to be 10000 rpm, the time was 10 minutes, the precipitate was taken, the precipitate was washed 3 times with ethyl acetate, the precipitate was washed 3 times with anhydrous ethanol, and transferred to an oven for drying, the temperature of the oven was controlled to be 65°C, the time was 15 hours, and sodium hyaluronate microspheres were obtained; The molecular weight of the sodium hyaluronate is 2 million Da; (2) Mixing: Add kaolin and sodium hyaluronate in a mass ratio of 30:11 into a ball mill for ball milling, control the ball-to-material ratio of the ball mill to 11:1, and rotate at 380 rpm for 1 h to obtain modified kaolin; mix the modified kaolin, sodium hyaluronate microspheres, and deionized water in a mass-to-volume ratio of 21 g:38 g:950 mL, stir at a stirring speed of 200 rpm for 2.5 h, and then transfer to a centrifuge for centrifugation at a speed of 8500 rpm for 10 min. Take the precipitate, wash it with deionized water for 3 times, and transfer it to an oven for drying at a temperature of 75°C for 29 h to obtain cross-linked microspheres; The molecular weight of the sodium hyaluronate is 200,000 Da; 2. Preparation of lactobacillus culture liquid: inoculate lactobacillus into MRS medium for single strain enrichment culture, incubate at 36°C for 45 hours to obtain primary seed liquid, then inoculate the primary seed liquid into MRS medium at an inoculum amount of 2.5% (volume fraction), incubate at 36°C for 22 hours to obtain secondary seed liquid, i.e., lactobacillus culture liquid; The preparation method of the MRS culture medium is as follows: 11 g of peptone, 5.5 g of beef extract, 4.2 g of yeast extract, 2 g of diammonium citrate, 22 g of glucose, 5.5 g of sodium acetate, 0.22 g of magnesium sulfate, 0.05 g of manganese sulfate, and 120 mL of distilled water are uniformly mixed, the pH is adjusted to 6.5, and the mixture is sterilized at 121° C. for 30 min to obtain the MRS culture medium; The lactobacillus liquid contains 5×10 9 CFU / mL; 3. Prepare yeast culture liquid: inoculate the yeast into YPD medium for single strain enrichment culture, incubate at 29°C for 22 hours to obtain primary seed liquid, then inoculate the primary seed liquid into YPD medium at an inoculum amount of 2.8% (volume fraction), incubate at 29°C for 35.5 hours to obtain secondary seed liquid, i.e. yeast culture liquid; The YDP medium is prepared by mixing 11 g yeast extract, 21 g peptone, 22 g glucose, and 110 mL distilled water, adjusting the pH to 6.5, and sterilizing at 121° C. for 30 min to obtain the YDP medium; The yeast liquid has a bacterial content of 9×10 8 CFU / mL; 4. Prepare a mixed bacterial solution: mix the lactobacillus bacterial solution and the yeast bacterial solution in a volume ratio of 1:1.2 to obtain a mixed bacterial solution; 5. Fermentation: Inoculate the mixed bacterial solution into the fermentation medium at an inoculum rate of 2.5% (volume fraction), and ferment at 37°C for 65 hours to obtain a fermentation liquid; 6. Mixing: Mix the fermentation liquid, sodium hyaluronate, glycerin, phenoxyethanol, ethylhexylglycerin, butylene glycol, stabilizer, dodecyl betaine and deionized water in a mass ratio of 25:1.8:4:0.8:0.4:4.5:3.2:1.8:78 and transfer them into a high-pressure homogenizer for homogenization. The pressure of the high-pressure homogenizer is controlled to 140 MPa and the homogenization times are 3 times to obtain a composite bacteria fermentation compound Chinese medicine scalp care composition; The molecular weight of the sodium hyaluronate is 100,000 Da; The preparation method of the stabilizer is as follows: betaine, acetylated sodium hyaluronate and kaolin are added into a ball mill in a mass ratio of 22:14:95 for ball milling, the ball-to-material ratio of the ball mill is controlled to be 11:1, the rotation speed is 380 rpm, and the ball milling is performed for 2 hours to obtain the stabilizer; The acetylated sodium hyaluronate has an acetyl substitution degree of 3.

[0012] This embodiment also provides an application of the aforementioned composite bacteria fermentation compound traditional Chinese medicine composition for scalp care.

[0013] Example 3 A composite bacterial fermentation compound traditional Chinese medicine scalp care composition, the preparation method of which comprises: 1. Prepare fermentation medium: select fresh, pest-free Rehmannia root, Ligustrum lucidum, Polygonatum sibiricum, Platycladus orientalis leaf, and Rhizoma Chuanxiong, clean them respectively and crush them into 150 mesh to obtain Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder, and Rhizoma Chuanxiong powder, then mix Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder, and Rhizoma Chuanxiong powder in a mass ratio of 30:25:20:40:20 to obtain mixed Chinese medicine powder; mix the mixed Chinese medicine powder, anhydrous ethanol, and deionized water in a mass volume ratio of 100g:800mL:120mL, soak them at room temperature for 4h, and perform ultrasonic Reflux extraction, control the temperature of ultrasonic reflux extraction to 70°C, the ultrasonic power to 120W, the time to 1.5h, then filter, take the filtrate, add cross-linked microspheres to the filtrate, control the mass volume ratio of the filtrate to the cross-linked microspheres to 850mL:8g, obtain a mixed solution, and then use a rotary evaporator to concentrate the mixed solution to 25% of the original volume, and then adjust the pH to 7 to obtain a concentrated solution, mix the concentrated solution and glucose at a mass volume ratio of 100mL:3.5g, sterilize at 100°C for 30min, and naturally cool to room temperature to obtain a fermentation medium; The preparation method of the cross-linked microspheres is: (1) Cross-linking: Sodium hyaluronate and a 2% sodium hydroxide aqueous solution were mixed at a mass volume ratio of 10 g:100 mL to obtain a sodium hyaluronate gel; Tween 80 and liquid paraffin were mixed at a mass volume ratio of 7 g:400 mL to obtain an oil phase; while stirring the oil phase at a stirring speed of 500 rpm, the water phase was added dropwise to the oil phase, the volume ratio of the oil phase to the water phase was controlled to be 400:560, the water phase was added dropwise for 50 min, and after the addition was completed, the mixture was transferred to a high-pressure homogenizer for homogenization, and the high-pressure homogenizer was controlled to be 1:1. The pressure is 150 MPa, and the homogenization times are 4 times to obtain a homogenous liquid; the homogenous liquid is mixed with 1,4-butanediol diglycidyl ether at a volume ratio of 950:8, stirred at a stirring speed of 500 rpm for 5 hours, allowed to stand for 15 hours, transferred to a centrifuge for centrifugation, the speed of the centrifuge is controlled to be 11000 rpm, the time is 10 minutes, the precipitate is taken, the precipitate is washed 4 times with ethyl acetate, the precipitate is washed 4 times with anhydrous ethanol, and transferred to an oven for drying, the temperature of the oven is controlled to be 70°C, the time is 16 hours, and sodium hyaluronate microspheres are obtained; The molecular weight of the sodium hyaluronate is 2.5 million Da; (2) Mixing: Add kaolin and sodium hyaluronate in a mass ratio of 30:12 into a ball mill for ball milling, control the ball-to-material ratio of the ball mill to 12:1, rotate at 400 rpm, and ball mill for 1.5 h to obtain modified kaolin; mix the modified kaolin, sodium hyaluronate microspheres, and deionized water in a mass-to-volume ratio of 22 g:40 g:1000 mL, stir at a stirring speed of 300 rpm for 3 h, and then transfer to a centrifuge for centrifugation, control the speed of the centrifuge to 9000 rpm, and centrifuge for 10 min. Take the precipitate, wash it with deionized water for 4 times, and transfer it to an oven for drying, control the temperature of the oven to 80°C, and dry it for 30 h to obtain cross-linked microspheres; The molecular weight of the sodium hyaluronate is 200,000 Da; 2. Preparation of lactobacillus culture liquid: inoculate lactobacillus into MRS medium for single strain enrichment culture, incubate at 37°C for 48 hours to obtain primary seed liquid, then inoculate the primary seed liquid into MRS medium at an inoculum amount of 3% (volume fraction), incubate at 37°C for 25 hours to obtain secondary seed liquid, i.e., lactobacillus culture liquid; The preparation method of the MRS culture medium is as follows: 12g of peptone, 6g of beef extract, 4.5g of yeast extract, 2.1g of diammonium citrate, 25g of glucose, 6g of sodium acetate, 0.25g of magnesium sulfate, 0.06g of manganese sulfate, and 130mL of distilled water are mixed evenly, the pH is adjusted to 6.6, and sterilized at 121°C for 30min to obtain the MRS culture medium; The lactobacillus liquid contains 7×10 9CFU / mL; 3. Prepare yeast culture liquid: inoculate yeast into YPD medium for single strain enrichment culture, incubate at 30°C for 24 hours to obtain primary seed liquid, then inoculate the primary seed liquid into YPD medium at an inoculum amount of 3% (volume fraction), incubate at 30°C for 36 hours to obtain secondary seed liquid, i.e. yeast culture liquid; The YDP medium is prepared by mixing 12 g yeast extract, 22 g peptone, 23 g glucose, and 120 mL distilled water, adjusting the pH to 6.6, and sterilizing at 121° C. for 30 min to obtain the YDP medium; The yeast liquid has a bacterial content of 1×10 9 CFU / mL; 4. Prepare a mixed bacterial solution: mix the lactobacillus bacterial solution and the yeast bacterial solution in a volume ratio of 1:1.5 to obtain a mixed bacterial solution; 5. Fermentation: Inoculate the mixed bacterial solution into the fermentation medium at an inoculum rate of 3% (volume fraction), and ferment at 38°C for 70 hours to obtain a fermentation solution; 6. Mixing: Mix the fermentation liquid, sodium hyaluronate, glycerin, phenoxyethanol, ethylhexylglycerin, butylene glycol, stabilizer, dodecyl betaine, and deionized water in a mass ratio of 30:2:5:1:1:5:3.5:2:80, and transfer them into a high-pressure homogenizer for homogenization. The pressure of the high-pressure homogenizer is controlled to be 150 MPa, and the homogenization times are 4 times to obtain a composite bacteria fermentation compound Chinese medicine scalp care composition; The molecular weight of the sodium hyaluronate is 100,000 Da; The preparation method of the stabilizer is as follows: betaine, acetylated sodium hyaluronate and kaolin are added into a ball mill at a mass ratio of 25:15:100 for ball milling, the ball-to-material ratio of the ball mill is controlled to be 12:1, the rotation speed is 400 rpm, and the ball milling is performed for 2.5 hours to obtain the stabilizer; The acetylated sodium hyaluronate has an acetyl substitution degree of 3.

[0014] This embodiment also provides an application of the aforementioned composite bacteria fermentation compound traditional Chinese medicine composition for scalp care.

[0015] Comparative Example 1 This comparative example is based on the preparation method of the composite bacterial fermentation compound Chinese medicine scalp care composition of Example 2, except that in the first step of preparing the fermentation medium, the addition of the cross-linked microspheres is omitted; the remaining operations are the same as those of Example 2; This comparative example also provides an application of the aforementioned composite bacteria fermentation compound traditional Chinese medicine composition for scalp care.

[0016] Comparative Example 2 This comparative example is based on the preparation method of the composite bacteria fermentation compound Chinese medicine scalp care composition of Example 2, and in the sixth step of the mixing step, the addition of the stabilizer is omitted; the remaining operations are the same as those of Example 2; This comparative example also provides an application of the aforementioned composite bacteria fermentation compound traditional Chinese medicine composition for scalp care.

[0017] Test Example 1 Skin irritation test 60 mice weighing 20-25 g were selected and divided into 6 groups, 10 mice in each group. The back hair of the mice was shaved clean, and the composite bacteria fermentation compound Chinese medicine scalp care composition prepared in Example 1-3 and Comparative Example 1-2 was applied to the back of the mice in Groups 1-5, respectively. The 6 groups of mice were used as blank controls and were not coated. After 4 hours, the backs of the mice in Groups 1-5 were washed, and the skin conditions of the coated parts were observed and recorded. The recorded results are as follows:

[0018] Test Example 2 Moisturizing Test 75 adult women aged 25-45 with dry and frizzy hair were selected and divided into 5 groups, with 15 women in each group. Groups 1-5 were given the composite bacteria fermented composite Chinese medicine scalp care compositions of Examples 1-3 and Comparative Examples 1-2, respectively, once a day for 30 days; In the experiment, the shampooing time and frequency of all volunteers were kept consistent. After shampooing, the compound Chinese medicine scalp care composition essence was applied to the scalp and hair and massaged for 2 minutes; After the test was completed, a questionnaire was conducted to investigate the degree to which the compound bacteria fermentation compound Chinese medicine scalp care composition improved dry and frizzy hair. The results are as follows:

[0019] Test Example 3: Detection of active ingredients in traditional Chinese medicine The total flavonoids, total polyphenols, and total sugar contents in the composite bacterial fermentation composite Chinese medicine scalp care composition of Examples 1-3 and Comparative Examples 1-2 were tested, and the test methods and test results are as follows: Detection method of total flavonoids content of active ingredients in traditional Chinese medicine: According to P30 of the first volume of the Chinese Pharmacopoeia, the total flavonoids were detected with reference to the total flavonoids determination method in hawthorn leaves of the Pharmacopoeia; Detection method of total polyphenol content of active ingredients in traditional Chinese medicine: Detection shall be carried out according to GB / T 8313-2008 (second method); Detection method of total sugar content of active ingredients in traditional Chinese medicine: Detect the total sugar content in the sample based on the sulfuric acid phenol method.

[0020]

[0021] Test Example 4 Hair follicle growth test The hair follicle growth effect of the composite bacterial fermentation compound Chinese medicine scalp care composition of Examples 1-3 and Comparative Examples 1-2 was tested, and the test method and test results are as follows: Adult hair follicles with hair loss were selected for the experiment. Intact and undamaged growth follicles were selected for culture under a dissecting microscope. The selected free follicles were carefully transferred into a 24-well culture plate, with one follicle per well. 0.5 mL of Williams' E serum-free medium was added and the culture medium was incubated at 37°C with 5% CO. 2 The hair follicles were suspended and cultured in a constant temperature incubator. After 24 hours, 60 hair follicles with obvious growth (>0.1 mm) were selected, and the length of each hair follicle was measured as the initial hair follicle length; then the 60 hair follicles were evenly divided into 6 groups, each with 10 hair follicles, marked as groups 1-6, and the composite bacteria fermented composite Chinese medicine scalp care composition of Examples 1-3 and Comparative Examples 1-2 was added to groups 1-5 for continued culture without changing the liquid. The composite bacteria fermented composite Chinese medicine scalp care composition was not added to group 6, and the culture was continued directly without changing the liquid.

[0022] After 15 days of continuous culture, the length of each hair follicle was measured as the final hair follicle length, and then the hair follicle hair growth rate was calculated. The calculation formula of the hair follicle hair growth rate is as follows: Hair follicle growth rate = (final hair follicle length - initial hair follicle length) / initial hair follicle length * 100%; Then the average hair growth rate of each group of hair follicles was calculated, and the results were as follows:

[0023] Test Example 5 Storage Test The composite bacterial fermented composite Chinese medicine scalp care compositions of Examples 1-3 and Comparative Examples 1-2 were left open at room temperature for 30 days, and then the contents of important active ingredients were tested according to the method of Test Example 3. The test results are as follows:

[0024] It can be seen from the results of Experimental Examples 1-5 that by adding cross-linked microspheres to the fermentation medium, the moisturizing effect, content of active ingredients of traditional Chinese medicine, hair follicle growth effect, and storage stability of the prepared composite bacterial fermentation compound traditional Chinese medicine scalp care composition can be improved; By adding a stabilizer to the mixed material, the moisturizing effect and storage stability of the prepared composite bacteria fermentation compound traditional Chinese medicine scalp care composition can be improved.

[0025] Unless otherwise specified, all percentages used in the present invention are by mass.

[0026] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composite bacterial fermentation compound Chinese medicine scalp care composition, characterized in that: The preparation method comprises the following steps: preparing fermentation medium, preparing lactobacillus liquid, preparing yeast liquid, preparing mixed liquid, fermenting, and mixing; The method for preparing the fermentation medium comprises selecting fresh, pest-free Rehmannia root, Ligustrum lucidum fruit, Polygonatum sibiricum, Platycladus orientalis leaf and Chuanxiong, washing them respectively and then crushing them to obtain Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder and Chuanxiong powder, then uniformly mixing the Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder and Chuanxiong powder to obtain a mixed Chinese medicine powder; mixing the mixed Chinese medicine powder, anhydrous ethanol and deionized water, soaking them at room temperature, performing ultrasonic reflux extraction, and then filtering, taking a filtrate, adding cross-linked microspheres to the filtrate to obtain a mixed solution, and then concentrating the mixed solution to 20-25% of the original volume by a rotary evaporator, and then adjusting the pH to 6-7 to obtain a concentrated solution, uniformly mixing the concentrated solution with glucose, sterilizing it, and naturally cooling it to room temperature to obtain a fermentation medium; The mixing material comprises mixing the fermentation liquid, sodium hyaluronate, glycerin, phenoxyethanol, ethylhexylglycerin, butylene glycol, stabilizer, dodecyl betaine and deionized water and homogenizing the mixture to obtain a composite bacterial fermentation composite Chinese medicine scalp care composition; The preparation method of the stabilizer comprises the following steps: mixing betaine, acetylated sodium hyaluronate and kaolin and then ball milling to obtain the stabilizer.

2. The composite bacterial fermentation compound traditional Chinese medicine scalp care composition according to claim 1, characterized in that: In the fermentation medium, the mass ratio of Rehmannia root powder, Ligustrum lucidum fruit powder, Polygonatum sibiricum powder, Platycladus orientalis leaf powder and Chuanxiong powder is 20-30:10-25:10-20:20-40:8-20; The mass volume ratio of mixed Chinese medicine powder, anhydrous ethanol and deionized water is 100g:750-800mL:80-120mL; The mass volume ratio of the filtrate to the cross-linked microspheres is 800-850 mL:7-8 g; The mass volume ratio of the concentrate to glucose is 100mL:2.5-3.5g; The temperature of the ultrasonic reflux extraction is 65-70° C., the ultrasonic power is 90-120 W, and the time is 1-1.5 h.

3. The composite bacterial fermentation compound traditional Chinese medicine scalp care composition according to claim 1, characterized in that: The preparation method of the cross-linked microspheres comprises the following steps: cross-linking and mixing; The cross-linking is performed by uniformly mixing sodium hyaluronate and sodium hydroxide aqueous solution to obtain sodium hyaluronate gel; uniformly mixing Tween 80 and liquid paraffin to obtain an oil phase; while stirring the oil phase, dripping the water phase into the oil phase, and after the dripping is completed, transferring the mixture into a high-pressure homogenizer for homogenization to obtain a homogenous liquid; mixing the homogenous liquid with 1,4-butanediol diglycidyl ether, stirring, standing, centrifuging, taking a precipitate, washing, and drying the precipitate to obtain sodium hyaluronate microspheres; In the mixing, kaolin and sodium hyaluronate are mixed and then ball-milled to obtain modified kaolin; modified kaolin, sodium hyaluronate microspheres and deionized water are mixed, stirred, and then centrifuged to obtain a precipitate, which is then washed and dried to obtain cross-linked microspheres.

4. The composite bacterial fermentation compound traditional Chinese medicine scalp care composition according to claim 3, characterized in that: In the cross-linking, the molecular weight of the sodium hyaluronate is 1.5 million to 2.5 million Da; The mass volume ratio of sodium hyaluronate and sodium hydroxide aqueous solution is 10g:50-100mL; The mass concentration of the sodium hydroxide aqueous solution is 2%; The mass volume ratio of Tween 80 and liquid paraffin is 7g:370-400mL; The volume ratio of oil phase to water phase is 380-400:510-560; The water phase is added for 40-50 min. The volume ratio of the homogenized liquid to 1,4-butanediol diglycidyl ether is 900-950:7-8.

5. The composite bacterial fermentation compound traditional Chinese medicine scalp care composition according to claim 3, characterized in that: In the mixture, the mass ratio of kaolin to sodium hyaluronate is 30:10-12; The mass volume ratio of modified kaolin, sodium hyaluronate microspheres, and deionized water is 20-22 g: 35-40 g: 900-1000 mL; The molecular weight of the sodium hyaluronate is 200,000 Da.

6. The composite bacterial fermentation compound traditional Chinese medicine scalp care composition according to claim 1, characterized in that: The mixed bacterial solution is prepared by uniformly mixing the lactobacillus bacterial solution and the yeast bacterial solution in a volume ratio of 1:1-1.5 to obtain a mixed bacterial solution; The lactobacillus liquid contains 1×10 9 -7×10 9 CFU / mL; The yeast liquid has a bacterial content of 6×10 8 -1×10 9 CFU / mL.

7. The composite bacterial fermentation compound traditional Chinese medicine scalp care composition according to claim 1, characterized in that: In the fermentation, the mixed bacterial liquid is inoculated into the fermentation medium at an inoculation rate of 2-3%, and fermented at 36-38° C. for 60-70 hours to obtain a fermentation liquid.

8. The composite bacterial fermentation compound traditional Chinese medicine scalp care composition according to claim 1, characterized in that: In the mixture, the mass ratio of fermentation liquid, sodium hyaluronate, glycerin, phenoxyethanol, ethylhexylglycerin, butylene glycol, stabilizer, dodecyl betaine and deionized water is 20-30:1.5-2:3-5:0.5-1:0.01-1:4-5:3-3.5:1.5-2:75-80; The molecular weight of the sodium hyaluronate is 100,000 Da; In the preparation of the stabilizer, the mass ratio of betaine, acetylated sodium hyaluronate and kaolin is 20-25:12-15:90-100; The acetylated sodium hyaluronate has an acetyl substitution degree of 3.

9. An application of the composite bacterial fermentation compound traditional Chinese medicine composition for scalp care according to any one of claims 1 to 8.

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