Slow-release Chinese herbal medicine skin care composition and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine cosmetics, and particularly discloses a slow-release type Chinese herbal medicine skin care composition and a preparation method thereof.The preparation method comprises the steps that rhizoma bletillae, coix seeds, pinellia ternate and other Chinese herbal medicines are smashed and then fermented twice through multiple zymophyte, then active ingredients in the Chinese herbal medicines are extracted, the active ingredients are wrapped with soya bean lecithin to form lipidosome easy to absorb, and the lipidosome is added into the Chinese herbal medicines to prepare the slow-release type Chinese herbal medicine skin care composition. The hydrogel formed by the chitosan and the beta-sodium glycerophosphate realizes slow release, and the prepared slow-release Chinese herbal medicine skin care composition has good beautifying and skin care effects and is suitable for people of different ages to use.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine cosmetics, in particular to a sustained-release traditional Chinese herbal medicine skin care composition and a preparation method thereof. Background Art

[0002] As people's living standards improve, their pursuit of beauty becomes deeper and deeper, and the cosmetics market is developing continuously. In recent years, with environmental pollution, excessive use of electronic products and increasing pressure in people's lives, people's skin problems are also getting worse. Skin problems such as dry skin, chapped skin, dark yellow skin, dark color, acne and blackheads trouble many beauty lovers, and people begin to use whitening and concealer cosmetics to improve these flaws. However, as many cheap chemical cosmetics contain carcinogens, excessive heavy metals and other safety hazards, people begin to pursue skin care products with natural ingredients. However, at present, existing natural skin care products have the problems of poor stability and easy inactivation of active skin care ingredients, poor retention of effective ingredients, which leads to the inability of the skin to fully absorb them and fail to achieve the expected skin care effect. Summary of the invention

[0003] The purpose of the present invention is to provide a method for preparing a sustained-release Chinese herbal medicine skin care composition to solve the problems of poor stability and easy inactivation of active skin care ingredients and poor retention of effective ingredients.

[0004] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0005] A method for preparing a sustained-release Chinese herbal medicine skin care composition, the preparation method comprising:

[0006] Step 1: Mix the Chinese herbal medicine composition with deionized water, inoculate the Chinese herbal medicine fermentation bacteria to ferment twice, boil the secondary fermentation product with high heat after the fermentation is completed, and then simmer with low heat for 80 to 100 minutes. After the simmering is completed, cool it, filter out the medicine liquid, immerse the medicine residue with deionized water, and simmer it again with low heat for 50 to 70 minutes. After the second simmering is completed, cool it, let it stand for 0.5 to 1.5 hours, filter out the medicine liquid, mix the two filtered medicine liquids, evaporate and concentrate them, and obtain the active ingredients of the Chinese herbal medicine;

[0007] Step 2: Mix soybean lecithin and ethanol, stir evenly at 50-60° C. to fully dissolve, add Chinese herbal medicine active ingredients and deionized water, and disperse by ultrasonication to obtain a Chinese herbal medicine active ingredient-lecithin ethanol solution, spin-coat the Chinese herbal medicine active ingredient-lecithin ethanol solution on a polytetrafluoroethylene plate, air-dry to obtain an active ingredient liposome film, immerse the active ingredient liposome film in HEPE buffer, seal and protect from light, and dissolve at 50-60° C. and 100-300 rpm mechanical shaking to obtain an active ingredient liposome solution;

[0008] Step 3: Mix glacial acetic acid and deionized water, stir evenly to obtain glacial acetic acid solution, add chitosan, stir evenly, ultrasonically disperse to obtain chitosan acetic acid solution, mix β-sodium glycerophosphate and deionized water, stir evenly, fully dissolve in a 55-65°C water bath to obtain β-sodium glycerophosphate solution, slowly drip β-sodium glycerophosphate solution into the chitosan acetic acid solution at 0-5°C and 200-400rpm and continue stirring until the mixed solution is clear, add active ingredient liposome solution, stir evenly, and let stand at 25-30°C for 3-5h to obtain a sustained-release Chinese herbal skin care composition.

[0009] As a limitation of the present invention, the Chinese herbal composition includes Bletilla striata, Coix seed, Pinellia ternata, wax gourd seeds, Dendrobium officinale, Honeysuckle, Clove, Ligustrum lucidum, Salvia miltiorrhiza, Tribulus terrestris, Momordica grosvenori, White Peony Root, Licorice, Broussonetia arvense, Rose, Asparagus cochinchinensis, Perilla frutescens, Leonurus japonicus, and Tangerine peel.

[0010] As limitation of the present invention, in the Chinese herbal medicine composition, the amount of each component is as follows, by mass: 28-32 parts of coix seed, 23-27 parts of bletilla striata, 18-22 parts of rose, 18-22 parts of clove, 18-22 parts of honeysuckle, 13-17 parts of pinellia, 13-17 parts of dendrobium, 13-17 parts of glossy privet, 13-17 parts of salvia miltiorrhiza, 8-12 parts of motherwort, 8-12 parts of tribulus terrestris, 8-12 parts of momordica grosvenori, 6-10 parts of white peony root, 6-10 parts of wax gourd seeds, 6-10 parts of liquorice, 6-10 parts of phyllostachys sylvestris, 6-10 parts of asparagus, 4-8 parts of perilla, and 4-8 parts of tangerine peel.

[0011] As a limitation of the present invention, the process of inoculating the Chinese herbal medicine composition with Chinese herbal medicine fermentation bacteria and fermenting twice is specifically as follows:

[0012] The Chinese medicine composition is washed, crushed, mixed with deionized water, inoculated with aerobic fermentation bacteria, and aerobically fermented at 28-32° C. for 6-8 days. After the aerobic fermentation, it is inactivated with steam at a steam temperature of 110-120° C. and a steam pressure of 0.08-0.12 MPa. After inactivation for 10-20 minutes, it is cooled to obtain a primary fermentation product.

[0013] The primary fermentation product is inoculated with anaerobic fermentation bacteria and anaerobically fermented at 35-40°C for 13-15 days. After the anaerobic fermentation, it is inactivated with steam at a steam temperature of 110-120°C and a steam pressure of 0.08-0.12MPa. After inactivation for 10-20 minutes, it is cooled to obtain a secondary fermentation product.

[0014] As a limitation of the present invention, the primary aerobic fermentation bacteria used is a combination of one or more of Bacillus licheniformis, Bacillus subtilis, Aspergillus niger and Trichoderma.

[0015] As a limitation of the present invention, the secondary anaerobic fermentation bacteria used are a combination of one or more of Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus acidophilus.

[0016] As a limitation of the present invention, the mass ratio of soybean lecithin, ethanol and Chinese herbal medicine active ingredient in the Chinese herbal medicine active ingredient-lecithin ethanol solution is (0.8-1.2):(7.0-7.4):(1.8-2.2).

[0017] As a limitation of the present invention, the mass ratio of chitosan, glacial acetic acid and deionized water in the chitosan acetic acid solution is (2.5-3.5):(0.5-0.8):(95-105).

[0018] As a limitation of the present invention, when preparing the sustained-release Chinese herbal skin care composition, the amounts of the components are: 18 to 22 parts of chitosan acetic acid solution, 3 to 5 parts of sodium β-glycerophosphate solution, and 9 to 11 parts of active ingredient liposome solution.

[0019] Pharmacological analysis:

[0020] Job's tears are cool in nature and sweet in taste. They enter the spleen, stomach, and lung meridians. They have the effects of diuresis and swelling, strengthening the spleen and removing dampness, clearing away heat and pus, and anti-inflammatory and analgesic.

[0021] Bletilla striata is cool in nature, bitter, sweet and astringent in taste, and enters the lung, liver and stomach meridians. It is effective in treating chapped hands and feet, swollen and painful ulcers, and carbuncles.

[0022] Rose is warm in nature and sweet in taste. It enters the liver and spleen meridians. It has the effects of regulating qi and relieving depression, calming the mind, harmonizing blood and nourishing blood, harmonizing blood and dispersing blood stasis, and astringing and stopping diarrhea.

[0023] Clove is warm in nature and spicy in taste. It enters the spleen and stomach meridians and is effective in treating abdominal distension, nausea, diarrhea, and toothache.

[0024] Honeysuckle is cold in nature and sweet in taste. It enters the lung and stomach meridians and has the effects of clearing away heat and detoxifying, dispersing wind and clearing heat.

[0025] Pinellia ternata is mild in nature and pungent in taste. It has the effects of relieving cough and inflammation, stimulating appetite and strengthening the spleen, reducing swelling and dispersing nodules. It is recorded in "Elbow Recipe" that mix Pinellia ternata powder with egg white and apply it on the affected area to treat carbuncles, back sores and breast sores.

[0026] Dendrobium is mild in nature and sweet in taste. It has the effects of nourishing yin and stomach, clearing away heat and promoting body fluid, and strengthening the kidneys and bones.

[0027] Fructus Ligustri Lucidi is mild in nature, bitter and sweet in taste, and has the effects of blackening the beard and hair, strengthening the tendons and strength, calming the mind and replenishing Qi.

[0028] Salvia miltiorrhiza is slightly cold in nature and bitter in taste. It can treat irregular menstruation, fetal movement before delivery, and bleeding in children. It has been verified by clinical doctors that the use of Salvia miltiorrhiza to treat skin diseases is effective.

[0029] Motherwort is mild in nature and sweet in taste. It can be applied externally on the face to treat dark skin, remove facial spots and wrinkles.

[0030] Tribulus terrestris is warm in nature and bitter in taste. It enters the lung, liver, and kidney meridians. It is effective in treating itching caused by urticaria, red and tearful eyes, and hyperactivity of liver fire.

[0031] Luo Han Guo is cool in nature and sweet in taste. It enters the lung and spleen meridians and has the effects of clearing heat and moistening the lungs, and lubricating the intestines and relieving constipation.

[0032] White peony root is slightly cold in nature, bitter and sour in taste, and enters the liver and spleen meridians. It has the effects of antibacterial and analgesic, calming liver yang, nourishing blood and nourishing yin.

[0033] Wax gourd seeds are slightly cold in nature and sweet in taste. They have the effects of moistening the lungs, resolving phlegm, eliminating carbuncles and promoting diuresis. "Rihuazi Bencao" records: Wax gourd seeds can remove wind-induced dark spots on the skin and moisturize the skin.

[0034] Licorice is mild in nature and sweet in taste. It is effective in treating sore throat caused by cold, sore throat caused by lung heat, and mouth ulcers and back carbuncles.

[0035] The leaves of the mulberry tree are cool in nature and sweet in taste. They are effective in treating dysentery with red and white discharge, fat and swollen face, and sudden loss of speech. It is recorded in "Shenghuifang" that the leaves of the mulberry tree can be mashed and applied to treat ringworm, sores, and itching.

[0036] Asparagus cochinchinensis is mild in nature and bitter in taste. It has the effects of dispelling wind and relieving cough, eliminating phlegm and relieving asthma. "Compendium of Materia Medica" records: Wash and mash the asparagus cochinchinensis, take the juice filtered with wine and drink it, which can treat carbuncle.

[0037] White perilla is warm in nature and pungent in taste. It has the effects of dispelling cold, relieving exterior symptoms, regulating qi and relieving fullness.

[0038] Tangerine peel is warm in nature and spicy in taste. It belongs to the lung meridian and has the effects of lowering qi, relieving cough, stopping diarrhea and promoting diuresis.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] The invention adopts a variety of plant Chinese herbal medicines as raw materials, the ingredients are natural and healthy, and it has the effects of whitening, moisturizing, removing spots and acne, and removing wrinkles.

[0041] The present invention extracts the active ingredients in Chinese herbal medicines by secondary fermentation and wraps them with soybean lecithin to form liposomes, which are conducive to penetrating the skin, facilitating human absorption, and effectively preventing the inactivation of the active ingredients. The hydrogel formed by chitosan acetic acid solution and β-sodium glycerophosphate solution is used as a liposome carrier, which can slowly release the liposomes and has the function of moisturizing. DETAILED DESCRIPTION

[0042] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] Chinese herbal medicines such as coix seed, bletilla striata, and rose were all from Beijing Tong Ren Tang. Fermentation bacteria such as Bacillus licheniformis and Trichoderma were all from Shaanxi Institute of Biological Agriculture. Soybean lecithin (purity: 98%), HEPE buffer (pH=5.5), chitosan (100-200 mpa·s), and sodium β-glycerophosphate (R094104) were provided by Shanghai En Chemical.

[0044] Example 1: A method for preparing a sustained-release Chinese herbal medicine skin care composition, specifically:

[0045] Step 1: Primary fermentation of Chinese herbal medicine

[0046] By mass, 30 parts of coix seeds, 25 parts of bletilla striata, 20 parts of rose, 20 parts of clove, 20 parts of honeysuckle, 15 parts of pinellia, 15 parts of dendrobium, 15 parts of glossy privet, 15 parts of salvia miltiorrhiza, 10 parts of motherwort, 10 parts of tribulus terrestris, 10 parts of momordica grosvenori, 8 parts of white peony root, 8 parts of wax gourd seeds, 8 parts of liquorice, 8 parts of phyllostachys arborvitae, 8 parts of asparagus, 5 parts of perilla and 5 parts of tangerine peel were washed and crushed, mixed with 500 parts of deionized water, inoculated with 1.5 parts of Bacillus licheniformis and 1.5 parts of Trichoderma, and fermented aerobically at 30°C for 7d. After the aerobic fermentation, they were inactivated with steam at a steam temperature of 115°C and a steam pressure of 0.10MPa. After inactivation for 15min, they were cooled to obtain a primary fermentation product.

[0047] Step 2: Secondary fermentation of Chinese herbal medicine

[0048] The primary fermentation product obtained in the previous step was inoculated with 4 parts of Lactobacillus plantarum by mass, and anaerobically fermented at 37°C for 14 days. After the anaerobic fermentation, it was inactivated with steam at a steam temperature of 115°C and a steam pressure of 0.10 MPa. After inactivation for 15 minutes, it was cooled to obtain a secondary fermentation product.

[0049] Step 3: Preparation of Chinese herbal active ingredients

[0050] By weight, the secondary fermentation product obtained in the previous step was boiled over high heat, then simmered over low heat for 90 minutes. After simmering, it was cooled and the medicinal liquid was filtered out. The residue was immersed in deionized water and simmered again over low heat for 60 minutes. After the second simmering, it was cooled, allowed to stand for 1 hour, and the medicinal liquid was filtered out. The filtered medicinal liquids were mixed, evaporated and concentrated to obtain the active ingredients of Chinese herbal medicine.

[0051] Step 4: Preparation of Chinese herbal active ingredient-liposome

[0052] Mix 1.0 part of soybean lecithin and 7.2 parts of ethanol by mass, stir evenly at 55°C to fully dissolve, add 2.0 parts of Chinese herbal medicine active ingredients and 3.0 parts of deionized water, and disperse by ultrasonic to obtain a Chinese herbal medicine active ingredient-lecithin ethanol solution, spin-coat the Chinese herbal medicine active ingredient-lecithin ethanol solution on a polytetrafluoroethylene plate, air-dry to obtain an active ingredient liposome film, immerse the obtained active ingredient liposome film in 10 parts of HEPE buffer, seal and protect from light, and dissolve by mechanical shaking at 55°C and 200rpm to obtain an active ingredient liposome solution.

[0053] Step 5: Preparation of sustained-release Chinese herbal skin care composition

[0054] By mass, 0.6 parts of glacial acetic acid and 100 parts of deionized water are mixed, stirred evenly to obtain a glacial acetic acid solution, 2.0 parts of chitosan are added, stirred evenly, and ultrasonically dispersed for 10 minutes to obtain a chitosan acetic acid solution, 5.6 parts of sodium β-glycerophosphate and 10 parts of deionized water are mixed, stirred evenly, and fully dissolved in a 60°C water bath to obtain a sodium β-glycerophosphate solution, 4.0 parts of sodium β-glycerophosphate solution are slowly dripped into 20 parts of the chitosan acetic acid solution at 2°C and 300rpm and continuously stirred until the mixed solution is clarified, 10 parts of the active ingredient liposome solution are added, stirred evenly, and allowed to stand at 27°C for 4 hours to obtain a sustained-release Chinese herbal skin care composition.

[0055] Embodiment 2: A method for preparing a sustained-release Chinese herbal medicine skin care composition, specifically:

[0056] Step 1: Primary fermentation of Chinese herbal medicine

[0057] By mass, 30 parts of coix seeds, 25 parts of bletilla striata, 20 parts of rose, 20 parts of clove, 20 parts of honeysuckle, 15 parts of pinellia, 15 parts of dendrobium, 15 parts of glossy privet, 15 parts of salvia miltiorrhiza, 10 parts of motherwort, 10 parts of tribulus terrestris, 10 parts of momordica grosvenori, 8 parts of white peony root, 8 parts of wax gourd seeds, 8 parts of liquorice, 8 parts of phyllostachys arborvitae, 8 parts of asparagus, 5 parts of perilla and 5 parts of tangerine peel were washed and crushed, mixed with 500 parts of deionized water, inoculated with 1.5 parts of Bacillus licheniformis and 1.5 parts of Trichoderma, and fermented aerobically at 30°C for 7d. After the aerobic fermentation, they were inactivated with steam at a steam temperature of 115°C and a steam pressure of 0.10MPa. After inactivation for 15min, they were cooled to obtain a primary fermentation product.

[0058] Step 2: Secondary fermentation of Chinese herbal medicine

[0059] The primary fermentation product obtained in the previous step was inoculated with 4 parts of Lactobacillus rhamnosus by mass, and anaerobically fermented at 37°C for 14 days. After the anaerobic fermentation, it was inactivated with steam at a steam temperature of 115°C and a steam pressure of 0.10 MPa. After inactivation for 15 minutes, it was cooled to obtain a secondary fermentation product.

[0060] Step 3: Preparation of Chinese herbal active ingredients

[0061] By weight, the secondary fermentation product obtained in the previous step was boiled over high heat, then simmered over low heat for 90 minutes. After simmering, it was cooled and the medicinal liquid was filtered out. The residue was immersed in deionized water and simmered again over low heat for 60 minutes. After the second simmering, it was cooled, allowed to stand for 1 hour, and the medicinal liquid was filtered out. The filtered medicinal liquids were mixed, evaporated and concentrated to obtain the active ingredients of Chinese herbal medicine.

[0062] Step 4: Preparation of Chinese herbal active ingredient-liposome

[0063] Mix 1.0 part of soybean lecithin and 7.2 parts of ethanol by mass, stir evenly at 55°C to fully dissolve, add 2.0 parts of Chinese herbal medicine active ingredients and 3.0 parts of deionized water, and disperse by ultrasonic to obtain a Chinese herbal medicine active ingredient-lecithin ethanol solution, spin-coat the Chinese herbal medicine active ingredient-lecithin ethanol solution on a polytetrafluoroethylene plate, air-dry to obtain an active ingredient liposome film, immerse the obtained active ingredient liposome film in 10 parts of HEPE buffer, seal and protect from light, and dissolve by mechanical shaking at 55°C and 200rpm to obtain an active ingredient liposome solution.

[0064] Step 5: Preparation of sustained-release Chinese herbal skin care composition

[0065] By mass, 0.6 parts of glacial acetic acid and 100 parts of deionized water are mixed, stirred evenly to obtain a glacial acetic acid solution, 2.0 parts of chitosan are added, stirred evenly, and ultrasonically dispersed for 10 minutes to obtain a chitosan acetic acid solution, 5.6 parts of sodium β-glycerophosphate and 10 parts of deionized water are mixed, stirred evenly, and fully dissolved in a 60°C water bath to obtain a sodium β-glycerophosphate solution, 4.0 parts of sodium β-glycerophosphate solution are slowly dripped into 20 parts of the chitosan acetic acid solution at 2°C and 300rpm and continuously stirred until the mixed solution is clarified, 10 parts of the active ingredient liposome solution are added, stirred evenly, and allowed to stand at 27°C for 4 hours to obtain a sustained-release Chinese herbal skin care composition.

[0066] Embodiment 3: A method for preparing a sustained-release Chinese herbal medicine skin care composition, specifically:

[0067] Step 1: Primary fermentation of Chinese herbal medicine

[0068] By mass, 30 parts of coix seeds, 25 parts of bletilla striata, 20 parts of rose, 20 parts of clove, 20 parts of honeysuckle, 15 parts of pinellia, 15 parts of dendrobium, 15 parts of glossy privet, 15 parts of salvia miltiorrhiza, 10 parts of motherwort, 10 parts of tribulus terrestris, 10 parts of momordica grosvenori, 8 parts of white peony, 8 parts of wax gourd seeds, 8 parts of liquorice, 8 parts of phyllostachys arborvitae, 8 parts of asparagus, 5 parts of perilla and 5 parts of tangerine peel were washed and crushed, mixed with 500 parts of deionized water, inoculated with 1.5 parts of Bacillus subtilis and 1.5 parts of Aspergillus niger, and fermented aerobically at 30°C for 7d. After the aerobic fermentation, steam sterilization was carried out with a steam temperature of 127°C and a steam pressure of 0.125MPa for 30min to obtain a primary fermentation product.

[0069] Step 2: Secondary fermentation of Chinese herbal medicine

[0070] The primary fermentation product obtained in the previous step was inoculated with 4 parts of Lactobacillus plantarum by mass, and anaerobically fermented at 37°C for 14 days. After the anaerobic fermentation, steam sterilization was performed at a steam temperature of 127°C and a steam pressure of 0.125 MPa for 30 minutes to obtain a secondary fermentation product.

[0071] Step 3: Preparation of Chinese herbal active ingredients

[0072] By weight, the secondary fermentation product obtained in the previous step was boiled over high heat, then simmered over low heat, and the filtrate was filtered out after simmering for 90 minutes. The filter residue was added with 500 parts of deionized water and boiled again over high heat, and simmered over low heat for 60 minutes, and the filtrate was filtered out. The filtrates from the two filtrations were mixed and concentrated in vacuo to obtain the active ingredients of the Chinese herbal medicine.

[0073] Step 4: Preparation of Chinese herbal active ingredient-liposome

[0074] Mix 1.0 part of soybean lecithin and 7.2 parts of ethanol by mass, stir evenly at 55°C to fully dissolve, add 2.0 parts of Chinese herbal medicine active ingredients and 3.0 parts of deionized water, and disperse by ultrasonic to obtain a Chinese herbal medicine active ingredient-lecithin ethanol solution, spin-coat the Chinese herbal medicine active ingredient-lecithin ethanol solution on a polytetrafluoroethylene plate, air-dry to obtain an active ingredient liposome film, immerse the obtained active ingredient liposome film in 10 parts of HEPE buffer, seal and protect from light, and dissolve by mechanical shaking at 55°C and 200rpm to obtain an active ingredient liposome solution.

[0075] Step 5: Preparation of sustained-release Chinese herbal skin care composition

[0076] By mass, 0.6 parts of glacial acetic acid and 100 parts of deionized water are mixed, stirred evenly to obtain a glacial acetic acid solution, 2.0 parts of chitosan are added, stirred evenly, and ultrasonically dispersed for 10 minutes to obtain a chitosan acetic acid solution, 5.6 parts of sodium β-glycerophosphate and 10 parts of deionized water are mixed, stirred evenly, and fully dissolved in a 60°C water bath to obtain a sodium β-glycerophosphate solution, 4.0 parts of sodium β-glycerophosphate solution are slowly dripped into 20 parts of the chitosan acetic acid solution at 2°C and 300rpm and continuously stirred until the mixed solution is clarified, 10 parts of the active ingredient liposome solution are added, stirred evenly, and allowed to stand at 27°C for 4 hours to obtain a sustained-release Chinese herbal skin care composition.

[0077] Based on Example 1, control experiments were carried out, specifically Comparative Example 1, Comparative Example 2 and Comparative Example 3, as described below:

[0078] Comparative Example 1: The Chinese herbal medicine was only subjected to aerobic fermentation, and the other conditions were the same as those in Example 1.

[0079] A method for preparing a sustained-release Chinese herbal medicine skin care composition, specifically comprising:

[0080] Step 1: Primary fermentation of Chinese herbal medicine

[0081] By mass, 30 parts of coix seeds, 25 parts of bletilla striata, 20 parts of rose, 20 parts of clove, 20 parts of honeysuckle, 15 parts of pinellia, 15 parts of dendrobium, 15 parts of glossy privet, 15 parts of salvia miltiorrhiza, 10 parts of motherwort, 10 parts of tribulus terrestris, 10 parts of momordica grosvenori, 8 parts of white peony root, 8 parts of wax gourd seeds, 8 parts of liquorice, 8 parts of phyllostachys arborvitae, 8 parts of asparagus, 5 parts of perilla and 5 parts of tangerine peel were washed and crushed, mixed with 500 parts of deionized water, inoculated with 1.5 parts of Bacillus licheniformis and 1.5 parts of Trichoderma, and fermented aerobically at 30°C for 7d. After the aerobic fermentation, they were inactivated with steam at a steam temperature of 115°C and a steam pressure of 0.10MPa. After inactivation for 15min, they were cooled to obtain a primary fermentation product.

[0082] Step 2: Preparation of active ingredients of Chinese herbal medicine

[0083] By weight, the fermentation product obtained in the previous step was boiled over high heat, then simmered over low heat for 90 minutes. After simmering, the mixture was cooled and filtered out. The residue was immersed in deionized water and simmered again over low heat for 60 minutes. After the second simmering, the mixture was cooled, allowed to stand for 1 hour, and the mixture was filtered out. The filtered mixtures were mixed, evaporated and concentrated to obtain the active ingredients of the Chinese herbal medicine.

[0084] Step 3: Preparation of Chinese herbal active ingredient-liposome

[0085] Mix 1.0 part of soybean lecithin and 7.2 parts of ethanol by mass, stir evenly at 55°C to fully dissolve, add 2.0 parts of Chinese herbal medicine active ingredients and 3.0 parts of deionized water, and disperse by ultrasonic to obtain a Chinese herbal medicine active ingredient-lecithin ethanol solution, spin-coat the Chinese herbal medicine active ingredient-lecithin ethanol solution on a polytetrafluoroethylene plate, air-dry to obtain an active ingredient liposome film, immerse the obtained active ingredient liposome film in 10 parts of HEPE buffer, seal and protect from light, and dissolve by mechanical shaking at 55°C and 200rpm to obtain an active ingredient liposome solution.

[0086] Step 4: Preparation of sustained-release Chinese herbal skin care composition

[0087] By mass, 0.6 parts of glacial acetic acid and 100 parts of deionized water are mixed, stirred evenly to obtain a glacial acetic acid solution, 2.0 parts of chitosan are added, stirred evenly, and ultrasonically dispersed for 10 minutes to obtain a chitosan acetic acid solution, 5.6 parts of sodium β-glycerophosphate and 10 parts of deionized water are mixed, stirred evenly, and fully dissolved in a 60°C water bath to obtain a sodium β-glycerophosphate solution, 4.0 parts of sodium β-glycerophosphate solution are slowly dripped into 20 parts of the chitosan acetic acid solution at 2°C and 300rpm and continuously stirred until the mixed solution is clarified, 10 parts of the active ingredient liposome solution are added, stirred evenly, and allowed to stand at 27°C for 4 hours to obtain a sustained-release Chinese herbal skin care composition.

[0088] Comparative Example 2: The Chinese herbal medicine was only subjected to anaerobic fermentation, and the other conditions were the same as those in Example 1.

[0089] A method for preparing a sustained-release Chinese herbal medicine skin care composition, specifically comprising:

[0090] Step 1: Primary fermentation of Chinese herbal medicine

[0091] By mass, 30 parts of coix seeds, 25 parts of bletilla striata, 20 parts of rose, 20 parts of clove, 20 parts of honeysuckle, 15 parts of pinellia, 15 parts of dendrobium, 15 parts of glossy privet, 15 parts of salvia miltiorrhiza, 10 parts of motherwort, 10 parts of tribulus terrestris, 10 parts of momordica grosvenori, 8 parts of white peony, 8 parts of wax gourd seeds, 8 parts of liquorice, 8 parts of phyllostachys arborvitae, 8 parts of asparagus, 5 parts of perilla and 5 parts of tangerine peel were washed and crushed, mixed with 500 parts of deionized water, inoculated with 4 parts of plant lactobacillus, and anaerobically fermented at 37°C for 14d. After the anaerobic fermentation, steam was used for inactivation at a steam temperature of 115°C and a steam pressure of 0.10MPa. After inactivation for 15min, the mixture was cooled to obtain a primary fermentation product.

[0092] Step 2: Preparation of active ingredients of Chinese herbal medicine

[0093] By weight, the fermentation product obtained in the previous step was boiled over high heat, then simmered over low heat for 90 minutes. After simmering, the mixture was cooled and filtered out. The residue was immersed in deionized water and simmered again over low heat for 60 minutes. After the second simmering, the mixture was cooled, allowed to stand for 1 hour, and the mixture was filtered out. The filtered mixtures were mixed, evaporated and concentrated to obtain the active ingredients of the Chinese herbal medicine.

[0094] Step 3: Preparation of Chinese herbal active ingredient-liposome

[0095] Mix 1.0 part of soybean lecithin and 7.2 parts of ethanol by mass, stir evenly at 55°C to fully dissolve, add 2.0 parts of Chinese herbal medicine active ingredients and 3.0 parts of deionized water, and disperse by ultrasonic to obtain a Chinese herbal medicine active ingredient-lecithin ethanol solution, spin-coat the Chinese herbal medicine active ingredient-lecithin ethanol solution on a polytetrafluoroethylene plate, air-dry to obtain an active ingredient liposome film, immerse the obtained active ingredient liposome film in 10 parts of HEPE buffer, seal and protect from light, and dissolve by mechanical shaking at 55°C and 200rpm to obtain an active ingredient liposome solution.

[0096] Step 4: Preparation of sustained-release Chinese herbal skin care composition

[0097] By mass, 0.6 parts of glacial acetic acid and 100 parts of deionized water are mixed, stirred evenly to obtain a glacial acetic acid solution, 2.0 parts of chitosan are added, stirred evenly, and ultrasonically dispersed for 10 minutes to obtain a chitosan acetic acid solution, 5.6 parts of sodium β-glycerophosphate and 10 parts of deionized water are mixed, stirred evenly, and fully dissolved in a 60°C water bath to obtain a sodium β-glycerophosphate solution, 4.0 parts of sodium β-glycerophosphate solution are slowly dripped into 20 parts of the chitosan acetic acid solution at 2°C and 300rpm and continuously stirred until the mixed solution is clarified, 10 parts of the active ingredient liposome solution are added, stirred evenly, and allowed to stand at 27°C for 4 hours to obtain a sustained-release Chinese herbal skin care composition.

[0098] Comparative Example 3: The active ingredients of Chinese herbal medicine are directly combined with the hydrogel, and the other conditions are the same as those in Example 1.

[0099] A method for preparing a sustained-release Chinese herbal medicine skin care composition, specifically comprising:

[0100] Step 1: Primary fermentation of Chinese herbal medicine

[0101] By mass, 30 parts of coix seeds, 25 parts of bletilla striata, 20 parts of rose, 20 parts of clove, 20 parts of honeysuckle, 15 parts of pinellia, 15 parts of dendrobium, 15 parts of glossy privet, 15 parts of salvia miltiorrhiza, 10 parts of motherwort, 10 parts of tribulus terrestris, 10 parts of momordica grosvenori, 8 parts of white peony root, 8 parts of wax gourd seeds, 8 parts of liquorice, 8 parts of phyllostachys arborvitae, 8 parts of asparagus, 5 parts of perilla and 5 parts of tangerine peel were washed and crushed, mixed with 500 parts of deionized water, inoculated with 1.5 parts of Bacillus licheniformis and 1.5 parts of Trichoderma, and fermented aerobically at 30°C for 7d. After the aerobic fermentation, they were inactivated with steam at a steam temperature of 115°C and a steam pressure of 0.10MPa. After inactivation for 15min, they were cooled to obtain a primary fermentation product.

[0102] Step 2: Secondary fermentation of Chinese herbal medicine

[0103] The primary fermentation product obtained in the previous step was inoculated with 4 parts of Lactobacillus plantarum by mass, and anaerobically fermented at 37°C for 14 days. After the anaerobic fermentation, it was inactivated with steam at a steam temperature of 115°C and a steam pressure of 0.10 MPa. After inactivation for 15 minutes, it was cooled to obtain a secondary fermentation product.

[0104] Step 3: Preparation of Chinese herbal active ingredients

[0105] By weight, the secondary fermentation product obtained in the previous step was boiled over high heat, then simmered over low heat for 90 minutes. After simmering, it was cooled and the medicinal liquid was filtered out. The residue was immersed in deionized water and simmered again over low heat for 60 minutes. After the second simmering, it was cooled, allowed to stand for 1 hour, and the medicinal liquid was filtered out. The filtered medicinal liquids were mixed, evaporated and concentrated to obtain the active ingredients of Chinese herbal medicine.

[0106] Step 4: Preparation of sustained-release Chinese herbal skin care composition

[0107] By mass, 0.6 parts of glacial acetic acid and 100 parts of deionized water are mixed, stirred evenly to obtain a glacial acetic acid solution, 2.0 parts of chitosan are added, stirred evenly, and ultrasonically dispersed for 10 minutes to obtain a chitosan acetic acid solution, 5.6 parts of sodium β-glycerophosphate and 10 parts of deionized water are mixed, stirred evenly, and fully dissolved in a 60°C water bath to obtain a sodium β-glycerophosphate solution, 4.0 parts of sodium β-glycerophosphate solution are slowly dripped into 20 parts of chitosan acetic acid solution at 2°C and 300rpm and continuously stirred until the mixed solution is clarified, 10 parts of Chinese herbal active ingredients are added, stirred evenly, and allowed to stand at 27°C for 4 hours to obtain a sustained-release Chinese herbal skin care composition.

[0108] Detection experiment:

[0109] The samples of sustained-release Chinese herbal skin care composition were prepared according to the preparation methods in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2 and Comparative Example 3, respectively.

[0110] Physiological toxicity test: 60 healthy adult male rats about 6 weeks old were selected as experimental animals, each rat had a monomer mass of 200-250g, and were randomly divided into 6 groups, with 10 rats in each group. The sustained-release Chinese herbal skin care composition samples were fed to the rats with food daily, and the rats were observed after 4 weeks.

[0111] Skin sensitivity test: 60 healthy adult male rats about 6 weeks old were selected as experimental animals, with a single rat mass of 200-250g. They were randomly divided into 6 groups, with 10 rats in each group. The hair on both sides of the rats' bodies were shaved to expose healthy skin. The sustained-release Chinese herbal skin care composition sample was applied to the same side of the rat's skin daily, and glycerin was applied to the other side. The rats were observed after 4 weeks.

[0112] Effect test: 30 female volunteers aged 20 to 50 years old were randomly selected from a certain area and randomly divided into 6 groups. The ITA° value of each volunteer's skin was measured using an ITA° skin colorimeter. The sustained-release Chinese herbal skin care composition samples were distributed and the users were informed to apply it on the skin once in the morning and once in the evening. After one month of use, the ITA° value of each volunteer's skin was measured again, the change rate of the average ITA° value of each group of volunteers' skin was calculated, and the skin care effect of the samples was evaluated.

[0113]

[0114] Conclusion: From the test results, the sustained-release Chinese herbal medicine composition has no physiological toxicity and skin toxicity, and has a good beauty and skin care effect. The users feel good and it has a certain effect on users of different age groups.

[0115] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

Claims

1. A method for preparing a sustained-release Chinese herbal medicine skin care composition, characterized in that: The preparation method is: Step 1: Mix the Chinese herbal medicine composition with deionized water, inoculate the Chinese herbal medicine fermentation bacteria to ferment twice, boil the secondary fermentation product with high heat after the fermentation is completed, and then simmer with low heat for 80 to 100 minutes. After the simmering is completed, cool it, filter out the medicine liquid, immerse the medicine residue with deionized water, and simmer it again with low heat for 50 to 70 minutes. After the second simmering is completed, cool it, let it stand for 0.5 to 1.5 hours, filter out the medicine liquid, mix the two filtered medicine liquids, evaporate and concentrate them, and obtain the active ingredients of the Chinese herbal medicine; Step 2: Mix soybean lecithin and ethanol, stir evenly at 50-60° C. to fully dissolve, add Chinese herbal medicine active ingredients and deionized water, and disperse by ultrasonication to obtain a Chinese herbal medicine active ingredient-lecithin ethanol solution, spin-coat the Chinese herbal medicine active ingredient-lecithin ethanol solution on a polytetrafluoroethylene plate, air-dry to obtain an active ingredient liposome film, immerse the active ingredient liposome film in HEPE buffer, seal and protect from light, and dissolve at 50-60° C. and 100-300 rpm mechanical shaking to obtain an active ingredient liposome solution; Step 3: Mix glacial acetic acid and deionized water, stir evenly to obtain glacial acetic acid solution, add chitosan, stir evenly, ultrasonically disperse to obtain chitosan acetic acid solution, mix β-sodium glycerophosphate and deionized water, stir evenly, fully dissolve in a 55-65°C water bath to obtain β-sodium glycerophosphate solution, slowly drip β-sodium glycerophosphate solution into the chitosan acetic acid solution at 0-5°C and 200-400rpm and continue stirring until the mixed solution is clear, add active ingredient liposome solution, stir evenly, and let stand at 25-30°C for 3-5h to obtain a sustained-release Chinese herbal skin care composition.

2. The method for preparing a sustained-release Chinese herbal medicine skin care composition according to claim 1, characterized in that: The Chinese herbal medicine composition includes bletilla striata, coix seed, pinellia, wax gourd seeds, dendrobium, honeysuckle, clove, glossy privet fruit, salvia miltiorrhiza, tribulus terrestris, momordica grosvenori, white peony root, liquorice, phyllostachys chinensis, rose, asparagus, perilla, motherwort, and tangerine peel.

3. The method for preparing a sustained-release Chinese herbal medicine skin care composition according to claim 2, characterized in that: In terms of mass, the amount of each component in the Chinese herbal medicine composition is: 28-32 parts of coix seed, 23-27 parts of bletilla striata, 18-22 parts of rose, 18-22 parts of clove, 18-22 parts of honeysuckle, 13-17 parts of pinellia, 13-17 parts of dendrobium, 13-17 parts of glossy privet, 13-17 parts of salvia miltiorrhiza, 8-12 parts of motherwort, 8-12 parts of tribulus terrestris, 8-12 parts of momordica grosvenori, 6-10 parts of white peony root, 6-10 parts of wax gourd seeds, 6-10 parts of liquorice, 6-10 parts of phyllostachys sylvestris, 6-10 parts of asparagus, 4-8 parts of perilla, and 4-8 parts of tangerine peel.

4. The method for preparing a sustained-release Chinese herbal medicine skin care composition according to claim 1, characterized in that: The process of inoculating the Chinese herbal medicine composition with Chinese herbal medicine fermentation bacteria and fermenting twice is as follows: The Chinese medicine composition is washed, crushed, mixed with deionized water, inoculated with aerobic fermentation bacteria, and aerobically fermented at 28-32° C. for 6-8 days. After the aerobic fermentation, it is inactivated with steam at a steam temperature of 110-120° C. and a steam pressure of 0.08-0.12 MPa. After inactivation for 10-20 minutes, it is cooled to obtain a primary fermentation product. The primary fermentation product is inoculated with anaerobic fermentation bacteria and anaerobically fermented at 35-40°C for 13-15 days. After the anaerobic fermentation, it is inactivated with steam at a steam temperature of 110-120°C and a steam pressure of 0.08-0.12MPa. After inactivation for 10-20 minutes, it is cooled to obtain a secondary fermentation product.

5. The method for preparing a Chinese medicinal plant extract fermentation liquid according to claim 4, characterized in that: The aerobic fermentation bacteria used are one or more of bacillus licheniformis, bacillus subtilis, aspergillus niger and trichoderma.

6. The method for preparing a sustained-release Chinese herbal medicine skin care composition according to claim 4, characterized in that: The anaerobic fermentation bacteria used are a combination of one or more of plant lactobacillus, rhamnosus lactobacillus and lactobacillus.

7. The method for preparing a sustained-release Chinese herbal medicine skin care composition according to claim 1, characterized in that: The mass ratio of soybean lecithin, ethanol and Chinese herbal medicine active ingredients in the Chinese herbal medicine active ingredients-lecithin ethanol solution is (0.8-1.2):(7.0-7.4):(1.8-2.2).

8. The method for preparing a sustained-release Chinese herbal medicine skin care composition according to claim 1, characterized in that: The mass ratio of chitosan, glacial acetic acid and deionized water in the chitosan acetic acid solution is (2.5-3.5):(0.5-0.8):(95-105).

9. The method for preparing a sustained-release Chinese herbal medicine skin care composition according to claim 1, characterized in that: When preparing the sustained-release Chinese herbal medicine skin care composition, the dosage of each component is: 18-22 parts of chitosan acetic acid solution, 3-5 parts of β-sodium glycerophosphate solution, and 9-11 parts of active ingredient liposome solution.

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