Application of pilea microphylla extract to preparation of anti-inflammatory preparation

Abstract

The invention provides application of a pilea microphylla extract, the pilea microphylla extract is applied to preparation of an anti-inflammatory preparation, and the pilea microphylla extract is obtained by extracting a pilea microphylla sample for 20-30 minutes at the pressure of 1-1.2 Kgf / cm < 2 > and the temperature of 121-135 DEG C by taking a phosphate buffer normal saline solution as an extracting agent.

Description

Technical Field

[0001] The present invention relates to a use of a small leaf cold water hemp extract, in particular to a use of the small leaf cold water hemp extract for preparing an anti-inflammatory preparation. Background Art

[0002] The skin is the outermost organ of the human body, but it is also very fragile. When human skin cells are stimulated by too many free radicals, the human immune system will cause an inflammatory response, which will lead to the aging of skin cells. Studies have shown that the human body's inflammatory response is related to nitric oxide (NO). Nitric oxide has a significant contribution to blood vessels, heart, nerve conduction, etc., and is an indispensable compound for protecting the human body. However, when the concentration of nitric oxide in the human body is too high, it is easy to cause an inflammatory response.

[0003] In the human body, the production of nitric oxide is mainly controlled by nitric oxide synthase (NOS). Nitric oxide synthase uses L-arginine in the body as the starting material and produces nitric oxide through a series of catalytic reactions in the human body. Nitric oxide synthase can be further divided into neural nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS). Neural nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS) are constitutive nitric oxide synthases, which usually play their role in specific physiological environments; among them, the former is responsible for the production of nitric oxide in the nerve tissue of the central nervous system and peripheral nervous system of the human body, while the latter produces nitric oxide in the blood vessels. The concentration of inducible nitric oxide synthase (iNOS) in the human body is usually very low; however, when the human body is stimulated (for example, by free radicals), it will be induced to proliferate in large quantities and produce a large amount of nitric oxide, thus causing inflammatory reactions and other harmful phenomena to the human body. Therefore, the inflammatory reaction of the human body is partly related to the induction of inducible nitric oxide synthase (iNOS). Therefore, if a suitable way can be found to reduce the expression of inducible nitric oxide synthase (iNOS) and convert the excess nitric oxide that has been produced into other substances, the inflammatory reaction of the human body can be effectively improved.

[0004] Currently, many skin care product manufacturers on the market achieve the above-mentioned effects by adding chemical additives into skin care products. However, today's consumers pay great attention to the naturalness of skin care product ingredients, and too many chemical additives may also cause additional burden on the skin.

[0005] In view of this, there is a real need to develop natural preparations that can improve skin conditions by providing anti-inflammatory benefits. Summary of the invention

[0006] To solve the above problems, the present invention aims to provide a use of a small leaf cold water hemp extract for preparing an anti-inflammatory preparation.

[0007] The elements and components described throughout the present invention use the quantifiers "a" or "an" only for convenience of use and to provide a general meaning of the scope of the present invention; in the present invention, they should be interpreted as including one or at least one, and a single concept also includes multiple situations, unless it is obvious that it means otherwise.

[0008] The use of the foliar cold water hemp extract of the present invention is used to prepare an anti-inflammatory preparation, wherein the foliar cold water hemp extract is extracted with a phosphate buffered saline solution as an extractant at a temperature between 1 and 1.2 kgf / cm 2 It is obtained by extracting a small leaf cold water hemp sample at a pressure between 121 and 135°C for 20 to 30 minutes.

[0009] Therefore, the use of the lobular cold water hemp extract of the present invention can be achieved through the active ingredients contained in the lobular cold water hemp extract. When the lobular cold water hemp extract is administered to a desired individual, the active ingredients can take effect on the epidermis or body of the desired individual, effectively inhibiting and / or reducing the inflammatory response by removing free radicals and nitric oxide in the epidermis or body, thereby providing an anti-inflammatory effect; and the lobular cold water hemp extract has low toxicity to cells and can even promote cell growth, so the lobular cold water hemp extract can be used to prepare anti-inflammatory preparations, which is the effect of the present invention.

[0010] The use of the foliar cold water hemp extract of the present invention, wherein the phosphate buffered saline solution includes sodium chloride with a concentration between 0.12 and 0.14 M and sodium dihydrogen phosphate with a concentration between 0.008 and 0.012 M. Thus, using the phosphate buffered saline with this component as an extractant, the active ingredients rich in the foliar cold water hemp sample can be completely extracted, thereby improving the anti-inflammatory effect of the foliar cold water hemp extract.

[0011] The use of the small leaf cold water hemp extract of the present invention, wherein the 2 The small leaf cold water hemp sample is extracted at a pressure between 10 and 20 °C. In this way, the active ingredients rich in the small leaf cold water hemp sample can be further completely extracted, thereby improving the anti-inflammatory effect of the small leaf cold water hemp extract.

[0012] The use of the small leaf cold water hemp extract of the present invention, wherein the small leaf cold water hemp extract is 100-300 μg / cm 2The dosage is administered to a desired individual. Thus, the dosage of the small leaf cold water hemp extract can more effectively reduce and / or inhibit the inflammatory response of the affected part, thereby obtaining a better anti-inflammatory effect.

[0013] The use of the lobular cold water hemp extract of the present invention, wherein the lobular cold water hemp extract is applied to a desired individual in the form of an application to inhibit inflammation in the desired individual. In this way, the user can easily obtain the active ingredients of the lobular cold water hemp extract to reduce and / or inhibit the inflammatory reaction of the affected part.

[0014] The use of the lobular cold water hemp extract of the present invention, wherein the lobular cold water hemp extract is applied to the required individual at a dosage of 100-300 μg per square centimeter of skin 2-3 times a day. In this way, the dosage of the lobular cold water hemp extract can more effectively reduce and / or inhibit the inflammatory reaction of the affected part, thereby obtaining a better anti-inflammatory effect.

[0015] The use of the extract of the small leaf cold water hemp of the present invention, wherein the preparation is in the form of milk, cream, liquid or gel to be used as a cosmetic skin care product or a skin dressing. In this way, the user can reduce and / or inhibit the inflammatory reaction of the affected part by applying it. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 : In experiment (A), the cell viability bar graph of the samples in groups A0 to A7;

[0017] Figure 2 : In experiment (B), the cell viability bar graph of the samples in groups B0 to B7;

[0018] Figure 3 : Histogram of DPPH clearance rates of samples from groups C0 to C10 in test (C);

[0019] Figure 4 : In test (D), the nitric oxide clearance rate bar graph of the samples in groups D0 to D9;

[0020] Figure 5 : In experiment (E), the results of immunofluorescence staining of the keratinocyte cell line HaCat;

[0021] Figure 6 : In experiment (F), the results of immunofluorescence staining of mouse macrophage RAW264.7 were obtained. DETAILED DESCRIPTION

[0022] In order to make the above and other objects, features and advantages of the present invention more obvious and understandable, preferred embodiments of the present invention are listed below and described in detail with reference to the accompanying drawings.

[0023] The "preparation" mentioned in the present invention refers to an active ingredient processed and modulated to form a specific dosage form, and includes a certain dose of medicines and cosmetics, and the "anti-inflammatory preparation" mentioned in the present invention has the activity of improving the inflammatory response of the user, which can be understood by technicians in the technical field to which the present invention belongs and will not be elaborated here.

[0024] The "Pilea microphylla" described in the present invention refers to a plant of the genus Pilea in the family Urticaeae, which is currently distributed in the middle altitude areas of the entire island of Taiwan, China. Its stem segments are green and white and transparent, and have the property of transmitting light under the sun, so it is also called "transparent grass". It also has common names such as small water hemp, small pearl grass, small-leafed cold water flower or small-leafed cold water grass.

[0025] The lobular cold water hemp extract of the present invention can remove free radicals and nitric oxide in the epidermis or the body and effectively inhibit and / or reduce inflammatory reactions, thereby providing an anti-inflammatory effect; and the lobular cold water hemp extract has low toxicity to cells and can even promote cell growth, so the lobular cold water hemp extract can be used to prepare anti-inflammatory preparations. The lobular cold water hemp extract can be combined with at least one pharmaceutically acceptable carrier to form the anti-inflammatory preparation, or other active ingredients with anti-inflammatory effects can be added to the anti-inflammatory preparation to be administered to a desired individual (e.g., 100-300 μg / cm 2 The dosage is applied to the skin of the desired individual), the carrier is an excipient or an additive, and preferably they can form various types of preparations such as liquid, emulsion, cream or gel, so as to be convenient for the desired individual to use.

[0026] The lobular cold water hemp extract of the present invention may refer to any extract obtained from a lobular cold water hemp sample, wherein the lobular cold water hemp extract may preferably be prepared by a method comprising the following steps: providing a lobular cold water hemp sample; using a phosphate buffered saline solution (PBS) as an extractant, and heating the lobular cold water hemp extract to a temperature between 1 and 1.2 kgf / cm 2 and a temperature between 121° C. and 135° C. to obtain a crude extract of small leaf cold water hemp; and concentrating the crude extract of small leaf cold water hemp to obtain the small leaf cold water hemp extract.

[0027] Specifically, the small-leaf cold-water hemp sample can be the whole plant of the small-leaf cold-water hemp. After obtaining the small-leaf cold-water hemp sample, the worker can directly extract it with the phosphate-buffered saline solution, or can dry the small-leaf cold-water hemp sample in advance before extraction, for example, drying to reduce the water content of the small-leaf cold-water hemp sample to 3-10%; in addition, the worker can also crush the small-leaf cold-water hemp sample in advance, for example, so that the particle size of the small-leaf cold-water hemp can be between 5 and 20 nm, so as to increase the contact surface area when the phosphate-buffered saline solution is used for extraction, thereby improving the subsequent extraction efficiency.

[0028] The phosphate buffered saline solution may include sodium chloride (NaCl) and sodium dihydrogen phosphate (NaH 2 PO 4 , sodium dihydrogen phosphate), and the pH value of the aqueous solution is generally neutral. For example, the phosphate buffered saline solution may include sodium chloride with a concentration between 0.12 and 0.14 M (mol / L) and sodium dihydrogen phosphate with a concentration between 0.008 and 0.012 M (mol / L), preferably may include sodium chloride with a concentration of 0.13 M and sodium dihydrogen phosphate with a concentration of 0.01 M, and the pH value of the phosphate buffered saline solution is 7.0.

[0029] In this embodiment, 200 ml of the extractant can be mixed with 100 g of the small leaf cold water hemp sample to obtain a mixture, which is then heated at a temperature between 1 and 1.2 kgf / cm 2 Between (for example, 1.033Kgf / cm 2 ) pressure and a temperature between 121 and 135°C (e.g., 127°C), and the extraction time may be between 20 and 30 minutes (e.g., 20 minutes), so that the active ingredients rich in the small-leaf cold water hemp sample can be completely dissolved in the extractant, thereby obtaining the small-leaf cold water hemp crude extract. In addition, in this embodiment, a filter hole with a pore size of 0.22 μm is used to filter the microorganisms. Moreover, the above-mentioned extraction step may preferably be carried out in a sterilizer to reduce the content of bacteria and / or microorganisms during the extraction process, so that the effect of the active ingredients contained in the small-leaf cold water hemp will not be affected by bacteria and / or microorganisms.

[0030] After obtaining the crude extract of cold water hemp from small leaves, the worker can freeze-dry the crude extract of cold water hemp from small leaves to obtain the cold water hemp extract from small leaves. Through this process, the active ingredients of the cold water hemp extract from small leaves can be more concentrated, so only a small amount of the cold water hemp extract from small leaves can be used to achieve the best effect. In this embodiment, a centrifuge is used to centrifuge at 12000 rpm for 20 minutes to remove the residue in the crude extract of cold water hemp from small leaves, and then freeze-drying is performed at a temperature between -50 and -80°C to obtain a dry powder, thereby obtaining the cold water hemp extract from small leaves.

[0031] In order to prove that the extract of the present invention is not toxic to human skin cells, and even has the activity of promoting skin cell regeneration, the following test was conducted:

[0032] (A) Effect of the extract of Hemp Leaf on the cell viability of keratinocytes

[0033] Please refer to Table 1. In this experiment, the lobular cold water hemp extract of the present invention was added to the keratinocyte strain HaCat. After culturing for 24 hours, the cell survival rate of each group of model cell strains was calculated using the MTT analysis method. The absorbance value of the control group A0 group (i.e., the group with the lobular cold water hemp extract concentration of 0 μg / mL) was taken as 100%, and the absorbance value results of the remaining groups were calculated, that is, the relative cell survival rate of each group of the test samples can be converted. The results are shown in FIG. Figure 1 shown.

[0034] Table 1. Samples to be tested in each group of this experiment.

[0035]

[0036]

[0037] Please refer to Figure 1 As shown, the relative cell survival rates of groups A1 to A4 were higher than those of group A0, indicating that the addition of medium-low concentrations (20 μg / mL to 80 μg / mL) of lobular cold water hemp extract can promote the survival of the keratinocyte cell line HaCat. As the concentration of lobular cold water hemp extract increased, the relative cell survival rates of groups A5 to A7 gradually increased, indicating that the addition of medium-high concentrations (100 μg / mL to 200 μg / mL) of lobular cold water hemp extract had no cytotoxicity to the keratinocyte cell line HaCat.

[0038] (B) Effect of the extract of Hemp Leaf on the cell viability of fibroblast cell lines

[0039] Please refer to Table 2. In this experiment, the lobular cold water hemp extract of the present invention was added to the fibroblast cell line HS68. After culturing for 24 hours, the cell survival rate of each group of model cell lines was calculated using the MTT analysis method. The absorbance value of the control group B0 (i.e., the group with the lobular cold water hemp extract concentration of 0 μg / mL) was taken as 100%, and the absorbance value results of the remaining groups were calculated, that is, the relative cell survival rate of each group of the test samples can be converted. The results are shown in FIG. Figure 2 shown.

[0040] Table 2. Samples to be tested in each group of this experiment.

[0041] Group Small leaf cold water hemp extract (μg / mL) Group B0 0 Group B1 20 Group B2 40 Group B3 60 Group B4 80 Group B5 100 Group B6 150 Group B7 200

[0042] Please refer to Figure 2 As shown, similar to the results obtained in experiment (A), the relative cell survival rates of groups B1 to B4 were higher than those of group B0, indicating that the addition of medium-low concentrations (20 μg / mL to 80 μg / mL) of the lobular cold water hemp extract can promote the survival of the fibroblast cell line HS68. As the concentration of the lobular cold water hemp extract increased, the relative cell survival rates of groups B5 to B7 gradually increased, indicating that the addition of medium-high concentrations (100 μg / mL to 200 μg / mL) of the lobular cold water hemp extract was not cytotoxic to the fibroblast cell line HS68.

[0043] Next, in order to confirm that the small leaf cold water hemp extract of the present invention can indeed be used to scavenge free radicals and thereby inhibit the expression of inducible nitric oxide synthase (iNOS), the following test was conducted:

[0044] (C) DPPH free radical scavenging efficacy test

[0045] This test uses the DPPH free radical scavenging analysis method to test the antioxidant capacity of the test sample. DPPH (α, α-diphenyl-β-pricrylhydrazyl) is a compound that can produce stable free radicals and is often used as an indicator for testing antioxidant capacity. DPPH appears blue-purple in alcohol solution. When the free radicals it produces are scavenged by the test sample, the color of the solution will change from blue-purple to yellow. Therefore, the higher the degree of decolorization of the DPPH solution, the stronger the antioxidant effect of the test sample. Please refer to Table 3. In this test, 50μl of each group of test solutions were taken, 1ml of DPPH solution and 0.95ml of Tris-HCl buffer solution were added in sequence, mixed evenly (oscillated), and then left to stand at room temperature for 30 minutes. The absorbance value at a wavelength of 517nm was measured by a spectrophotometer. Then, the absorbance values ​​of each group were substituted into the following formula to obtain the DPPH scavenging rate of each group of test samples. In addition, the C1 group of this test was a negative control group, in which only 75% ethanol aqueous solution was added, and no small leaf cold water hemp extract was added; while the C2 group was a positive control group, in which 40 μg / mL vitamin C was added, but no small leaf cold water hemp extract was added. Since the DPPH solution has a strong absorbance value at a wavelength of 517nm, the lower the measured absorbance value, the stronger the DPPH scavenging rate (antioxidant power) of the sample to be tested.

[0046]

[0047] Table 3. Samples to be tested in each group of this experiment.

[0048]

[0049]

[0050] Please refer to Figure 3 As shown, the DPPH scavenging rate of the foliar cold water hemp extract of the present invention is positively correlated with its concentration, and when the concentration is above 200 μg / mL (i.e., Group C8 to Group C10), the DPPH scavenging rate reaches more than 50%, indicating that the foliar cold water hemp extract of the present invention can indeed effectively scavenge free radicals and thereby inhibit the expression of inducible nitric oxide synthase (iNOS).

[0051] Next, in order to confirm that the small leaf cold water hemp extract of the present invention can indeed be used to remove the generated nitric oxide and thus inhibit / reduce the inflammatory response, the following test was conducted:

[0052] (D) Nitric oxide scavenging efficacy test

[0053] This test uses sodium nitroprusside (SNP) scavenging analysis to test the nitric oxide scavenging effect of the sample to be tested. Sodium nitroprusside (SNP) is a substance that can stably produce nitric oxide in a solution and is often used as an indicator for testing the nitric oxide scavenging effect. Please refer to Table 4. In this test, 0.12 ml of each group of test solutions was added with 0.08 ml of SNP solution (12.5 mM, dissolved in PBS) and reacted at room temperature for 30 minutes. Then, 0.02 ml of sulfonamide solution (1% by weight, dissolved in 5% phosphoric acid aqueous solution) and 0.02 ml of N-(naphthyl)ethylenediamine dihydrochloride aqueous solution (1% by weight) were added, and the mixture was evenly shaken for 5 minutes, and the absorbance value at a wavelength of 550 nm was continuously measured. Then, the absorbance values ​​of each group were substituted into the following formula to obtain the nitric oxide scavenging rate of each group of test samples. The lower the absorbance value, the stronger the nitric oxide scavenging ability of the test sample. Furthermore, group D1 of the present experiment was a negative control group, in which only phosphate buffered saline (PBS) aqueous solution was added, and no C. microphylla cold water hemp extract was added.

[0054]

[0055] Table 4. Samples to be tested in each group of this experiment.

[0056] Group Small leaf cold water hemp extract (μg / mL) Group D0 0 Group D1 0 Group D2 20 Group D3 40 Group D4 60 Group D5 80 Group D6 100 Group D7 200 Group D8 400 Group D9 600

[0057] Please refer to Figure 4 As shown, the nitric oxide clearance rate of the lobular cold water hemp extract of the present invention is positively correlated with its concentration, and when the concentration is above 400 μg / mL (i.e., Group D8 to Group D9), the nitric oxide clearance rate reaches above 50%, indicating that the lobular cold water hemp extract of the present invention can indeed effectively clear the generated nitric oxide, thereby eliminating and / or reducing the inflammatory response of the human body.

[0058] Next, in order to confirm that the small leaf cold water hemp extract of the present invention can promote the anti-aging performance of skin cells, the following test was conducted:

[0059] (E) Effect of small leaf cold water hemp extract on the expression of anti-aging protein Sirt6

[0060] In this experiment, 0 μg / mL, 100 μg / mL and 200 μg / mL of the lobular cold water hemp extract were added to the keratinocyte strain HaCat, and after 24 hours of culture, the anti-aging protein Sirt6 was stained using immunofluorescence staining to observe the effect of the lobular cold water hemp extract of the present invention on the expression of the anti-aging protein Sirt6; wherein DAPI is a cell nucleus staining agent that can clearly mark the position of the cell nucleus.

[0061] Please refer to Figure 5 As shown, in the control group without adding the lobular cold water hemp extract of the present invention, the anti-aging protein Sirt6 was almost not expressed; as the concentration of the lobular cold water hemp extract increased, the expression of the anti-aging protein Sirt6 was significantly enhanced, indicating that the lobular cold water hemp extract of the present invention can indeed enhance the expression of the anti-aging protein Sirt6, thereby providing an anti-aging effect.

[0062] Next, in order to confirm that the small leaf cold water hemp extract of the present invention can provide anti-inflammatory effects, the following tests were conducted:

[0063] (F) Effect of small leaf cold water hemp extract on the expression of transcription factor NF-κB

[0064] The transcription factor NF-κB is a representative factor that can regulate inflammatory responses. Its function is to regulate pro-inflammatory genes in innate immune cells and to regulate the activation and differentiation of inflammatory T cells. In this experiment, mouse macrophages RAW264.7 were used as a model cell line. After lipopolysaccharide (LPS) was used to induce the immune response of the cells, 100 μg / mL and 200 μg / mL of lobular cold water hemp extract were added to the mouse macrophages RAW264.7 that had produced the immune response. After 6 hours of culture, the transcription factor NF-κB was stained using immunofluorescence staining to observe the effect of the lobular cold water hemp extract of the present invention on the expression of the transcription factor NF-κB. Among them, DAPI is a nuclear stain that can clearly mark the location of the cell nucleus.

[0065] Please refer to Figure 6 As shown, the control group is a group in which the lobular cold water hemp extract of the present invention is not added and lipopolysaccharide (LPS) is not used to induce the immune response of the cells. The expression of the transcription factor NF-κB is very weak and is located in the cytoplasm of the cells; after the lipopolysaccharide (LPS) is used to induce the immune response of the cells, it can be observed that the fluorescence intensity of the transcription factor NF-κB is significantly enhanced, and the fluorescence is mostly concentrated in the position of the cell nucleus, indicating that the mouse macrophage RAW264.7 is indeed affected by lipopolysaccharide (LPS) and produces an immune response; then, after the lobular cold water hemp extract of different concentrations (i.e., 100μg / mL and 200μg / mL) is used to treat the mouse macrophage RAW264.7 that has produced an immune response, the fluorescence intensity of the transcription factor NF-κB is significantly reduced, and the fluorescence leaves the position of the cell nucleus and disperses in the cytoplasm, indicating that the lobular cold water hemp extract of the present invention can indeed effectively inhibit and / or reduce the inflammatory response, thereby providing an anti-inflammatory effect.

[0066] In summary, the use of the lobular cold water hemp extract of the present invention can be achieved through the active ingredients contained in the lobular cold water hemp extract. When the lobular cold water hemp extract is administered to a desired individual, the active ingredients can take effect on the epidermis or body of the desired individual, and effectively inhibit and / or reduce the inflammatory response by removing free radicals and nitric oxide in the epidermis or body, thereby providing an anti-inflammatory effect; and the lobular cold water hemp extract has low toxicity to cells and can even promote cell growth, so the lobular cold water hemp extract can be used to prepare anti-inflammatory preparations, which is the effect of the present invention.

[0067] Although the present invention has been disclosed using the above-mentioned preferred embodiments, they are not intended to limit the present invention. Any person skilled in the art may make various changes and modifications to the above-mentioned embodiments without departing from the spirit and scope of the present invention, and these changes and modifications still fall within the technical scope protected by the present invention. Therefore, the protection scope of the present invention shall include all changes within the meaning and equivalent scope of the claims.

Claims

1. A use of a small leaf cold water hemp extract, characterized in that: The invention is used for preparing an anti-inflammatory preparation, wherein the small leaf cold water hemp extract is obtained by extracting a small leaf cold water hemp sample at a temperature between 121 and 135° C. for 20 to 30 minutes using a phosphate buffered saline solution as an extractant.

2. The use of the small leaf cold water hemp extract according to claim 1, characterized in that The phosphate buffered saline solution includes sodium chloride with a concentration between 0.12 and 0.14 M and sodium dihydrogen phosphate with a concentration between 0.008 and 0.012 M.

3. The use of the small leaf cold water hemp extract according to claim 1, characterized in that Between 1~1.2 Kgf / cm 2 Extract the small leaf cold water hemp sample under pressure between.

4. The use of the small leaf cold water hemp extract according to claim 1, characterized in that The small leaf cold water hemp extract is 100-300 μg / cm 2 The dosage is administered to an individual in need.

5. The use of the small leaf cold water hemp extract according to any one of claims 1 to 3, characterized in that The small leaf cold water hemp extract is applied to a desired individual in the form of an application to suppress inflammation in the desired individual.

6. The use of the small leaf cold water hemp extract according to claim 5, characterized in that The small leaf cold water hemp extract is administered to the individual in need at a dosage of 100 to 300 μg per square centimeter of skin 2 to 3 times a day.

7. The use of the small leaf cold water hemp extract according to claim 5, characterized in that: The preparation is in the form of an emulsion, cream, liquid or gel for use as a cosmetic care product or as a skin dressing.

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