Microbial agent-enzyme preparation composition and method for rapidly treating kitchen waste

Abstract

The invention belongs to the field of kitchen waste degradation, and discloses a microbial agent-enzyme preparation composition and a method for rapidly treating kitchen waste. The microbial agent-enzyme preparation composition comprises a compound microbial agent I, a compound microbial agent II and a compound enzyme preparation; the compound enzyme preparation comprises alpha-amylase, saccharifying enzyme, neutral protease, lipase and cellulase; the compound microbial agent I comprises bacillus subtilis, bacillus amyloliquefaciens and lactobacillus fermentum; the compound microbial agent II comprises bacillus subtilis, bacillus licheniformis, lactobacillus fermentum and candida cylindrica. The invention further discloses a liquid kitchen waste treatment method which takes kitchen waste as an object and adopts compound enzyme pretreatment and compound microorganism secondary aerobic treatment as a core. The technical scheme provided by the invention is suitable for aerobic digestion treatment of the kitchen waste, degradation can be completed within 48 hours, and tail water formed after degradation reaches the discharge standard, so that on-site, rapid and harmless treatment of the kitchen waste is realized.

Description

Technical Field

[0001] The invention relates to the field of restaurant and kitchen waste degradation, and in particular to a microbial agent-enzyme preparation composition and a method for quickly processing restaurant and kitchen waste. Background Art

[0002] With the continuous improvement of national environmental protection policy requirements, the harmless, resource-based and reduced-volume treatment of food waste ("three-treatment") has become one of the focuses of urban governance. my country produces more than 90 million tons of food waste annually, with the "four highs" characteristics of high concentration, high salt, high oil and high moisture. With the government's increasing attention and large-scale investment in food waste classification and treatment technology, there has been significant progress in food waste treatment technology.

[0003] In recent years, a new model of decentralized or on-site rapid treatment of food waste is emerging in some major cities in my country. This new model is centered on schools, government agencies, military units, etc., and installs a solid waste treatment equipment with a corresponding treatment capacity and a high degree of automation to centrally treat the food waste generated at any time, and solid-state fermentation of food waste is processed into organic fertilizer, avoiding the secondary pollution caused by long-distance transportation of food waste. However, long-term practice has shown that this model also has obvious shortcomings. Due to the poor fluidity and thermal conductivity of solid materials, it is easy to cause poor contact between materials and microorganisms (including enzyme preparations), resulting in uneven biodegradation, and a long treatment time. Once the fermentation technology is not mastered well, it will affect the quality of organic fertilizers, making it difficult to completely kill insect eggs and pathogens, or causing problems such as plant seedling burns during fertilizer application, ultimately causing secondary pollution.

[0004] In the prior art, some research institutions or enterprises have developed methods for treating food waste in liquid form, such as the on-site rapid food waste treatment process disclosed in CN112718803A, which organically combines microbial degradation technology, enzymatic hydrolysis technology and sewage treatment technology. However, during the treatment process, no targeted and efficient degradation is performed according to the different components in the food waste at different stages. Instead, the composite microbial agent and the composite enzyme preparation are directly added at the hydrolysis stage, resulting in incomplete biological reaction of the food waste, insufficient material conversion, and ineffective degradation. In addition, the secondary aerobic degradation rate is low, which affects the effluent COD after treatment and the quality of the discharged sewage is unstable. Summary of the invention

[0005] In view of the defects of the prior art, the present invention provides a microbial agent-enzyme preparation composition and a method for rapidly treating kitchen waste. The composition is compounded with microorganisms of different species to form a composite microbial combination suitable for different aerobic treatment stages, and works together with the composite enzyme preparation to achieve efficient degradation of kitchen waste.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] In the first aspect, the present invention provides a microbial agent-enzyme preparation composition for degrading food waste, comprising a composite microbial agent one, a composite microbial agent two and a composite enzyme preparation; the composite enzyme preparation comprises α-amylase, saccharifying enzyme, neutral protease, lipase and cellulase; the composite microbial agent one comprises Bacillus subtilis, Bacillus amyloliquefaciens and Lactobacillus fermentation; the composite microbial agent two comprises Bacillus subtilis, Bacillus licheniformis, Lactobacillus fermentation and Candida columnaris.

[0008] The two composite microbial agents contained in the microbial agent-enzyme preparation composition provided by the present invention are respectively suitable for different aerobic treatment stages, and work together with the composite enzyme preparation to quickly and efficiently degrade food waste.

[0009] The food waste referred to in the present invention refers to the residues, wastes and processing wastes from households, schools, institutions, canteens, catering and primary processing industries of agricultural products, mainly including vegetable processing wastes, fruit peels and cores, leftovers and waste edible oils and fats.

[0010] Composite microbial agent 1 includes Bacillus subtilis, Bacillus amyloliquefaciens and Lactobacillus fermentation, which is suitable for the early aerobic treatment stage of food waste and can quickly decompose large molecular organic matter. Bacillus subtilis can secrete proteases and lipases to help decompose complex organic matter and provide nitrogen sources and energy for Lactobacillus fermentation. Bacillus amyloliquefaciens secretes amylase to convert starch into small molecular sugars, which can be used as a carbon source for the other two strains to promote their growth. Lactobacillus fermentation produces lactic acid by fermenting sugars, lowering the pH value of the environment, creating a suitable growth environment for Bacillus subtilis and Bacillus amyloliquefaciens, and inhibiting pathogenic microorganisms. The metabolites released by these microorganisms during growth, such as organic acids, antimicrobial peptides, and extracellular polysaccharides, can not only inhibit pathogenic microorganisms, but also act as signal molecules to regulate the physiological activities of other microorganisms and promote symbiotic relationships. For example, lactic acid produced by Lactobacillus fermentation can activate the expression of genes related to stress response in Bacillus subtilis and Bacillus amyloliquefaciens, enhancing their environmental adaptability.

[0011] Composite microbial agent II includes Bacillus subtilis, Bacillus licheniformis, Lactobacillus fermentum and Candida columnaris, which are suitable for the later aerobic treatment stage of food waste and can completely decompose food waste. Bacillus subtilis and Bacillus licheniformis can metabolize and secrete a variety of enzymes, such as protease, lipase, cellulase and xylanase, to synergistically decompose complex organic matter and provide rich nutrients for other strains. Lactobacillus fermentum reduces the pH value of the environment through lactic acid fermentation, inhibits harmful microorganisms, and provides a suitable acidic environment for other microorganisms. Candida columnaris can utilize oils and fats to help decompose fats and other difficult-to-degrade organic substances.

[0012] Composite microbial agent 1 and composite microbial agent 2 regulate the fermentation system through the metabolic activities of microorganisms, creating an environment that is conducive to their own reproduction while inhibiting the growth of miscellaneous bacteria, and secreting antibacterial substances to ensure that they occupy a dominant position in the treatment of food waste and form a stable dominant bacterial community. The bacterial species in the agent support each other and synergistically accelerate the decomposition and stabilization of garbage, achieving efficient degradation and resource utilization of food waste.

[0013] The enzymes of the complex enzyme preparation may exist in the form of free enzymes or immobilized enzymes. The form of the complex enzyme preparation may be selected according to specific application requirements and treatment targets. In some cases, the complex enzyme preparation may contain both free enzymes and immobilized enzymes to give full play to the advantages of both.

[0014] Preferably, the mass ratio of the α-amylase, saccharifying enzyme, neutral protease, lipase and cellulase is 1-2:1-2:1-2:1-2:2-3.

[0015] Optionally, the α-amylase activity is 0.5-5wU / g; the saccharifying enzyme activity is 0.5-10wU / g; the neutral protease activity is 0.1-10wU / g; the lipase activity is 0.5-10wU / g; the cellulase activity is 0.5-5wU / g;

[0016] More preferably, the mass ratio of the α-amylase, saccharifying enzyme, neutral protease, lipase and cellulase is 2:1:1:1:3.

[0017] Optionally, the α-amylase activity is 2wU / g; the saccharifying enzyme activity is 5wU / g; the neutral protease activity is 5wU / g; the lipase activity is 5wU / g; the cellulase activity is 2wU / g;

[0018] Preferably, the Bacillus subtilis in the composite microbial agent one is at least one of Bacillus subtilis TRIIM1.01067, Bacillus subtilis TRIIM 1.01035, and Bacillus subtilis TRIIM 1.01075; the Bacillus amyloliquefaciens is at least one of Bacillus amyloliquefaciens TRIIM 1.00312, Bacillus amyloliquefaciens TRIIM 1.00308, and Bacillus amyloliquefaciens TRIIM 1.00324; the Lactobacillus fermentum is at least one of Lactobacillus fermentum TRIIM 1.14009, Lactobacillus fermentum TRIIM 1.14005, and Lactobacillus fermentum TRIIM 1.14003.

[0019] Preferably, the Bacillus subtilis in the second composite microbial agent is at least one of Bacillus subtilis TRIIM1.01046, Bacillus subtilis TRIIM 1.01077, and Bacillus subtilis TRIIM 1.01080; the Bacillus licheniformis is at least one of Bacillus licheniformis TRIIM 1.00420, Bacillus licheniformis TRIIM 1.00418, and Bacillus licheniformis TRIIM 1.00423; the Lactobacillus fermentum is at least one of Lactobacillus fermentum TRIIM 1.14021, Lactobacillus fermentum TRIIM 1.14010, and Lactobacillus fermentum TRIIM 1.14026; the columnar Candida is at least one of columnar Candida TRIIM 2.10100, columnar Candida TRIIM 2.10102, and columnar Candida TRIIM 2.10108.

[0020] Preferably, the composite microbial agent 1 and the composite microbial agent 2 further comprise biologically acceptable microbial agent carriers and / or excipients.

[0021] The composite microbial agent 1 and the composite microbial agent 2 provided by the present invention can be prepared into different dosage forms by selecting suitable carriers and / or excipients to improve the stability, activity and use effect of the agent to meet different production needs. The dosage form of the agent includes but is not limited to liquid, powder, granule, tablet and capsule; the excipients include but are not limited to glycerol, polyethylene glycol, starch, glucose, maltodextrin, sucrose, lactose, skim milk powder, gum arabic and chitosan; the carrier includes but is not limited to culture medium, water, starch, cellulose and gelatin.

[0022] More preferably, the carrier of the composite microbial agent one is a liquid culture medium; the composite microbial agent one is prepared by mixing the Bacillus subtilis, Bacillus amyloliquefaciens and Lactobacillus fermentation at a mass ratio of 1-2:3-4:1-2 of the liquid culture medium after enrichment culture.

[0023] More preferably, the carrier used for the composite microbial agent 2 is a liquid culture medium; the composite microbial agent 2 is prepared by enriching and culturing the Bacillus subtilis, Bacillus licheniformis, Lactobacillus fermentum and Candida columnaris and mixing them according to the mass ratio of the liquid culture medium of 2-3:1-2:2-3:2-3.

[0024] More preferably, the carrier of the composite microbial agent one is a liquid culture medium; the composite microbial agent one is prepared by mixing the Bacillus subtilis, Bacillus amyloliquefaciens and Lactobacillus fermentation according to a mass ratio of 1:3:1 of the liquid culture medium after enrichment culture.

[0025] More preferably, the carrier used for the composite microbial agent 2 is a liquid culture medium; the composite microbial agent 2 is prepared by enriching and culturing the Bacillus subtilis, Bacillus licheniformis, Lactobacillus fermentum and Candida columnaris and mixing them in a mass ratio of 2:1:2:2 of the liquid culture medium.

[0026] In a second aspect, the present invention also provides the use of the above-mentioned microbial agent-enzyme preparation composition for degrading food waste in aerobic digestion treatment of food waste.

[0027] In a third aspect, the present invention further provides a method for rapidly processing food waste, comprising the following steps:

[0028] S1: After crushing the kitchen waste, add water to dilute it, the material-water ratio is 1:4-5, and beat it until all the liquid passes through a 40-60 mesh sieve, and then send it to a hydrolysis tank for standby;

[0029] S2: hydrolysis treatment: adjusting the organic matter concentration in the hydrolysis tank to make the COD less than 15000 mg / L; adding the composite enzyme preparation in the above-mentioned microbial agent-enzyme preparation composition equivalent to 0.05%-1% of the weight of the kitchen waste into the hydrolysis tank, starting stirring for enzymolysis, controlling the temperature at 25-40° C., stirring speed at 50-100 rpm, and treating for 1-3 hours to obtain a hydrolyzate;

[0030] S3: primary aerobic: the hydrolyzate obtained in S2 is pumped into a primary aerobic tank for aerobic aeration for 20-24 hours to obtain primary aerobic treated garbage liquid; the tank is pre-installed with a composite filler covering 60%-80% of the effective volume, the composite microbial agent 1 in the above-mentioned microbial agent-enzyme preparation composition is pre-dosed, and the microorganisms are fixed and biofilmed; the access amount of the composite microbial agent 1 is a live bacterial count greater than or equal to 1×10 based on the weight of the kitchen waste. 6 cfu / kg;

[0031] S4: primary sedimentation: the garbage liquid obtained from the primary aerobic treatment in S3 is pumped into the primary sedimentation tank, left to stand for 20-40 minutes, the supernatant overflows into the secondary aerobic tank, and the sediment is pumped back to the primary aerobic tank;

[0032] S5: Secondary aerobic: The supernatant from the overflow of S4 enters the secondary aerobic tank and is aerobically aerated for 20-24 hours to obtain the secondary aerobic treated garbage liquid; the secondary aerobic tank is pre-installed with a composite filler covering 60%-80% of the effective volume, and the composite microbial agent II in the above-mentioned microbial agent-enzyme preparation composition is pre-dosed, and the microorganisms are fixed and biofilmed; the access amount of the composite microbial agent II is calculated based on the weight of the kitchen waste, and the number of viable bacteria is greater than or equal to 1×10 6 cfu / kg;

[0033] S6: Secondary sedimentation: The secondary aerobic treatment waste liquid obtained in S5 is pumped into the secondary sedimentation tank, left to stand for 20-40 minutes, the supernatant overflows into the MBR tank for deep treatment, and the sediment is pumped back to the secondary aerobic tank;

[0034] S7: MBR deep treatment: The treated liquid overflowing from the secondary sedimentation tank into the MBR tank is treated by the MBR membrane to obtain sewage that meets the discharge standards.

[0035] The kitchen waste in S1 can be sorted in advance to remove the parts that are not kitchen waste.

[0036] The amount of the composite microbial agent in S3 and S5 is calculated in terms of viable bacteria. In actual operation, when different forms of microbial agents are added to the food waste, the amount of viable bacteria can be used as a reference for conversion.

[0037] The combined fillers in S3 and S5 are used for biofilm, which can effectively cut bubbles, improve the transfer rate and utilization rate of oxygen, make the water and air biofilm fully exchanged, and efficiently treat organic matter. Soft fillers or semi-soft fillers can be used according to actual needs, including but not limited to soft fiber fillers, foam fillers, and biological rope fillers.

[0038] In S7, an MBR membrane assembly is pre-installed in the MBR tank, and the layout of the membrane assembly is configured according to the process requirements. Optionally, the MBR membrane assembly includes but is not limited to a plate-and-frame type, a spiral-wound type, a circular tube type, a hollow fiber type, and a capillary type.

[0039] The present invention divides the degradation process into two stages according to the difficulty and degradation rate differences of organic matter in the kitchen waste during the degradation process, and uses composite microbial agents in a targeted manner according to the composition of organic matter in each stage. At the same time, wet crushing and pulping pretreatment is adopted to crush the kitchen waste into a liquid state, and enzymolysis and biodegradation are carried out in the liquid state to expand the contact area between organic macromolecules and microorganisms and enzymes. In the two-stage aerobic treatment device, adsorption composite fillers are added, which can effectively fix microorganisms, biological enzymes and organic macromolecules to increase the effective surface area. Through these measures, the kitchen waste treatment method provided by the present invention can completely decompose the kitchen waste, and the treatment process maintains a high degradation efficiency, shortens the total degradation time, and ensures the stability of the quality of the treated sewage.

[0040] Preferably, the material-water ratio in S2 is 1:4.5; in S3, a composite enzyme preparation equivalent to 0.6% of the weight of the kitchen waste is added to the hydrolysis tank, the temperature is controlled at 35°C, the stirring speed is 100 rpm, and the treatment time is 3 hours; the access amount of the composite microbial agent 1 in S4 is a viable bacterial count greater than or equal to 1×10 8 cfu / kg; the access amount of the composite microbial agent 2 in S6 is that the number of viable bacteria is greater than or equal to 1×108 cfu / kg. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0042] Figure 1 The figure is a schematic diagram of the on-site rapid kitchen waste treatment process of the present invention. DETAILED DESCRIPTION

[0043] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] The sources of the strains used in the present invention are as follows:

[0045] Bacillus subtilis TRIIM 1.01067 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on December 15, 2022, and its deposit number is TRIIM 1.01067; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0046] Bacillus subtilis TRIIM 1.01035 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on January 10, 2023, and its deposit number is TRIIM 1.01035; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0047] Bacillus subtilis TRIIM 1.01077 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on January 10, 2023, and its deposit number is TRIIM 1.01077; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0048] Bacillus amyloliquefaciens TRIIM 1.00312 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on December 15, 2022, and its deposit number is TRIIM 1.00312; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0049] Bacillus amyloliquefaciens TRIIM 1.00308 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on February 6, 2023, and its deposit number is TRIIM 1.00308; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0050] Lactobacillus fermentum TRIIM 1.14009 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on October 24, 2022, and its deposit number is TRIIM 1.14009; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0051] Lactobacillus fermentum TRIIM 1.14005 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on December 15, 2022, and its deposit number is TRIIM 1.14005; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0052] Lactobacillus fermentum TRIIM 1.14021 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on March 1, 2023, and its deposit number is TRIIM 1.14021; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0053] Lactobacillus fermentum TRIIM 1.14010 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on February 17, 2023, and its deposit number is TRIIM 1.14010; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0054] Bacillus licheniformis TRIIM 1.00420 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on September 6, 2022, and its deposit number is TRIIM 1.00420; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0055] Bacillus licheniformis TRIIM 1.00418 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on February 17, 2023, and its deposit number is TRIIM 1.00418; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0056] Columnar Candida TRIIM 2.10100 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on January 10, 2023, and its deposit number is TRIIM 2.10100; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0057] Columnar Candida TRIIM 2.10102 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on March 1, 2023, and its deposit number is TRIIM 2.10102; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0058] Columnar Candida TRIIM 2.10097 was deposited in the Strain Center of Tianjin Institute of Industrial Microbiology on October 24, 2022, and its deposit number is TRIIM 2.10097; the deposit address is No. 21, Xinsheng Road, West District, Tianjin Development Zone.

[0059] The enzyme sources used in the present invention are as follows:

[0060] α-Amylase (2wU / g), saccharifying enzyme (5wU / g), lipase (5wU / g), cellulase (2wU / g) and neutral protease (5wU / g) were all purchased from Tianjin Noo Enzyme Productivity Promotion Co., Ltd.

[0061] Unless otherwise specified, other experimental materials and instruments can be purchased through commercial channels.

[0062] The combined filler and MBR membrane assembly in the aerobic treatment of the present invention are:

[0063] Aerobic combined environmentally friendly filler, model ZHTLY, unit diameter 150mm, spacing 80mm, purchased from Guangzhou Lvye Environmental Protection Equipment Co., Ltd.

[0064] MBR membrane assembly, MBR hollow fiber membrane, effective membrane area 16 m2, pore size 0.001-0.1 μm, outer diameter 0.5-2 mm, inner diameter 0.3-1.4 mm, purchased from Shandong Wanxi Environmental Protection Technology Co., Ltd.

[0065] The preparation method of the composite microbial agent of the present invention is:

[0066] The activated Bacillus subtilis, Bacillus amyloliquefaciens, Lactobacillus fermentation, Bacillus licheniformis and Candida columnaris are inoculated into the culture medium at an inoculation rate of 1% respectively for expansion culture to a stable period; the corresponding culture medium is mixed according to the mass ratio to obtain a composite microbial agent.

[0067] The culture medium used for Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus licheniformis is LB medium. LB medium: 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride, 1000 mL of distilled water, pH 7.0, and autoclaved at 121°C for 20 min.

[0068] The culture medium used for fermentation of lactobacillus is MRS broth medium. MRS broth medium: weigh 55.2 g in 1 L of distilled water, heat and boil until completely dissolved, divide into test tubes, and sterilize at 121°C for 20 minutes.

[0069] The culture medium used for columnar Candida is YPD medium. YPD medium: 20g peptone, 10g yeast extract, 900mL distilled water, add the above ingredients into distilled water, sterilize at 121℃ for 20min, and then add 100mL 20g glucose (add after the glucose solution is sterilized).

[0070] The detection method involved in the present invention is:

[0071] Chemical oxygen demand (COD): Based on HJ / T 399-2007 Rapid digestion spectrophotometric method for determination of chemical oxygen demand in water.

[0072] Ammonia nitrogen NH3-N: According to HJ 535-2009 Water quality - Determination of ammonia nitrogen - Nessler's reagent spectrophotometric method.

[0073] Suspended solids SS: Based on GB / T 11901-1989 Gravimetric method for determination of suspended solids in water.

[0074] pH value: Based on the electrode method for determining pH value of water quality in HJ 1147-2020.

[0075] Example 1

[0076] This embodiment provides a microbial agent-enzyme preparation composition for degrading food waste.

[0077] The composite enzyme preparation is composed of alpha-amylase, saccharifying enzyme, neutral protease, lipase and cellulase in a mass ratio of 1:2:2:1:3.

[0078] The composition of the composite microbial agent 1 is a liquid culture medium of Bacillus subtilis TRIIM 1.01067, a liquid culture medium of Bacillus amyloliquefaciens TRIIM 1.00312, and a liquid culture medium of Lactobacillus fermentum TRIIM 1.14009 in a mass ratio of 2:4:1. The viable bacterial count of the composite microbial agent 1 is about 2.5×10 8 cfu / mL.

[0079] The composition of the composite microbial agent 2 is a liquid culture medium of Bacillus subtilis TRIIM 1.01046, a liquid culture medium of Bacillus licheniformis TRIIM 1.00420, a liquid culture medium of Lactobacillus fermentum TRIIM 1.14021, and a liquid culture medium of Candida columnaris TRIIM 2.10100 in a mass ratio of 2:1:2:2. The viable count of the composite microbial agent 1 is about 3.5×10 8 cfu / mL.

[0080] Example 2

[0081] This embodiment provides a microbial agent-enzyme preparation composition for degrading food waste.

[0082] The composite enzyme preparation is composed of alpha-amylase, saccharifying enzyme, neutral protease, lipase and cellulase in a mass ratio of 2:1:1:1:3.

[0083] The composition of the composite microbial agent 1 is a liquid culture medium of Bacillus subtilis TRIIM 1.01035, a liquid culture medium of Bacillus amyloliquefaciens TRIIM 1.00308, and a liquid culture medium of Lactobacillus fermentum TRIIM 1.14005 in a mass ratio of 1:3:1. The viable bacterial count of the composite microbial agent 1 is about 1×10 8 cfu / mL.

[0084] The composition of the composite microbial agent II is a liquid culture medium of Bacillus subtilis TRIIM 1.01077, a liquid culture medium of Bacillus licheniformis TRIIM 1.00418, a liquid culture medium of Lactobacillus fermentum TRIIM 1.14010, and a liquid culture medium of Candida columnaris TRIIM 2.10102 in a mass ratio of 2:1:2:2. The viable bacterial count of the composite microbial agent II is about 3.5×10 8 cfu / mL.

[0085] Example 3

[0086] This embodiment provides a microbial agent-enzyme preparation composition for degrading food waste.

[0087] The composite enzyme preparation is composed of alpha-amylase, saccharifying enzyme, neutral protease, lipase and cellulase in a mass ratio of 2:1:1:2:2.

[0088] The composition of the composite microbial agent 1 is a liquid culture medium of Bacillus subtilis TRIIM 1.01075, a liquid culture medium of Bacillus amyloliquefaciens TRIIM 1.00324, and a liquid culture medium of Lactobacillus fermentum TRIIM 1.14003 in a mass ratio of 1:3:1. The viable bacterial count of the composite microbial agent 1 is about 1×10 8 cfu / mL.

[0089] The composition of the composite microbial agent II is a liquid culture medium of Bacillus subtilis TRIIM 1.01080, a liquid culture medium of Bacillus licheniformis TRIIM 1.00423, a liquid culture medium of Lactobacillus fermentum TRIIM 1.14026, and a liquid culture medium of Candida columnaris TRIIM 2.10108 in a mass ratio of 3:2:3:3. The viable bacterial count of the composite microbial agent II is about 5×10 8 cfu / mL.

[0090] Example 4

[0091] This embodiment provides a method for rapidly treating food waste, using the microbial agent-enzyme preparation composition provided in Example 1, and specifically treating according to the following steps:

[0092] (1) Remove chopsticks, napkins, plastic, large bones, etc. from kitchen waste to avoid damaging knives.

[0093] (2) Weigh 100 kg of the degradable part of the food waste, crush it, add water to dilute it, and the material-water ratio is 1:4. Start the pulping equipment and adjust the speed of the pulping equipment to 9000 r / min until all the liquid passes through a 50-mesh sieve and is sent to a hydrolysis tank for standby use.

[0094] (3) Adjusting the organic matter concentration in the hydrolysis tank to make the COD less than 15000 mg / L. Adding a composite enzyme preparation equivalent to 0.08% of the weight of the kitchen waste into the hydrolysis tank, turning on the stirring to perform enzymolysis, controlling the temperature at 35° C., the stirring speed at 80 rpm, and the processing time for 2 hours to obtain a hydrolyzate.

[0095] (4) The hydrolyzate obtained in step (3) is pumped into a primary aerobic tank, in which a composite filler covering 70% of the effective volume is pre-installed, a composite microbial agent 1 is pre-doped, and the microorganisms are fixed and biofilmed, and aerobic aeration is performed for 24 hours to obtain a primary aerobic treated garbage liquid; the amount of composite microbial agent 1 added is based on the weight of the food waste, and the inoculation amount is 1×10 6 cfu / kg.

[0096] (5) The primary aerobic treated sewage obtained in step (4) is pumped into the primary sedimentation tank, left to stand for 30 minutes, the supernatant overflows into the secondary aerobic tank, and the sediment is pumped back to the primary aerobic tank.

[0097] (6) The overflow supernatant of step (5) enters the secondary aerobic tank and is aerobically aerated for 24 hours to obtain the secondary aerobic treated garbage liquid. The secondary aerobic tank is pre-installed with a composite filler covering 70% of the effective volume, and the composite microbial agent II is pre-dosed, and the microorganisms have been fixed and biofilmed; the amount of composite microbial agent II is calculated based on the weight of the food waste, and the inoculation amount is 1×10 6 cfu / kg.

[0098] (7) The secondary aerobic treated garbage liquid obtained in step (6) is pumped into a secondary sedimentation tank and allowed to stand for 30 minutes. The supernatant overflows into the MBR tank for deep treatment, and the sediment is pumped back to the secondary aerobic tank.

[0099] (8) The treated liquid overflowing from the secondary sedimentation tank flows into the MBR tank, which is treated by the MBR membrane and finally obtains sewage that meets the discharge standards.

[0100] The COD, degradation rate, NH3-N, SS and pH value of the sewage before and after the treatment of food waste were tested, as shown in Table 1.

[0101] Example 5

[0102] This embodiment provides a method for rapidly treating food waste, using the microbial agent-enzyme preparation composition provided in Example 2, and specifically treating the food waste in the following steps:

[0103] (1) Remove chopsticks, napkins, plastic, large bones, etc. from kitchen waste to avoid damaging knives.

[0104] (2) Weigh 100 kg of the degradable portion of the food waste, crush it, add water to dilute it, and the material-water ratio is 1:4.5. Adjust the speed of the pulping equipment to 6000 r / min until all the liquid passes through a 40-mesh sieve and is sent to a hydrolysis tank for standby use.

[0105] (3) Adjusting the organic matter concentration in the hydrolysis tank to make the COD less than 15000 mg / L. Adding a composite enzyme preparation equivalent to 0.05% of the weight of the kitchen waste into the hydrolysis tank, turning on the stirring to perform enzymolysis, controlling the temperature at 35° C., the stirring speed at 50 rpm, and the processing time for 2 hours to obtain a hydrolyzate.

[0106] (4) The hydrolyzate obtained in step (3) is pumped into a primary aerobic tank, in which a composite filler covering 70% of the effective volume is pre-installed, a composite microbial agent 1 is pre-doped, and the microorganisms are fixed and biofilmed. The aerobic aeration is performed for 24 hours to obtain a primary aerobic treated garbage liquid. The amount of composite microbial agent 1 is calculated based on the weight of the food waste, and the inoculation amount is 1×10 8 cfu / kg.

[0107] (5) The primary aerobic treated sewage obtained in step (4) is pumped into the primary sedimentation tank, left to stand for 30 minutes, the supernatant overflows into the secondary aerobic tank, and the sediment is pumped back to the primary aerobic tank.

[0108] (6) The overflowed supernatant of step (5) enters the secondary aerobic tank and is aerobically aerated for 24 hours to obtain the secondary aerobic treated garbage liquid. The secondary aerobic tank is pre-installed with a composite filler covering 70% of the effective volume, and the composite microbial agent II is pre-dosed, and the microorganisms have been fixed and biofilmed. The amount of composite microbial agent II is calculated based on the weight of the food waste, and the inoculation amount is 1×10 8 cfu / kg.

[0109] (7) The secondary aerobic treated garbage liquid obtained in step (6) is pumped into a secondary sedimentation tank and allowed to stand for 30 minutes. The supernatant overflows into the MBR tank for deep treatment, and the sediment is pumped back to the secondary aerobic tank.

[0110] (8) The treated liquid overflowing from the secondary sedimentation tank flows into the MBR tank, which is treated by the MBR membrane and finally obtains sewage that meets the discharge standards.

[0111] The COD, degradation rate, NH3-N, SS and pH value of the sewage before and after the treatment of food waste were tested, as shown in Table 1.

[0112] Example 6

[0113] This embodiment provides a method for rapidly treating food waste, using the microbial agent-enzyme preparation composition provided in Example 3, and specifically treating the food waste in the following steps:

[0114] (1) Remove chopsticks, napkins, plastic, large bones, etc. from kitchen waste to avoid damaging knives.

[0115] (2) Weigh 100 kg of the degradable portion of the food waste, crush it, add water to dilute it, and the material-water ratio is 1:5. Adjust the speed of the pulping equipment to 8000 r / min until all the liquid passes through a 60-mesh sieve and is sent to a hydrolysis tank for standby use.

[0116] (3) Adjusting the organic matter concentration in the hydrolysis tank to make the COD less than 15000 mg / L. Adding a composite enzyme preparation equivalent to 0.08% of the weight of the kitchen waste into the hydrolysis tank, turning on the stirring to perform enzymolysis, controlling the temperature at 35° C., the stirring speed at 100 rpm, and the processing time for 3 hours to obtain a hydrolyzate.

[0117] (4) The hydrolyzate obtained in step (3) is pumped into a primary aerobic tank, in which a composite filler covering 70% of the effective volume is pre-installed, a composite microbial agent 1 is pre-doped, and the microorganisms are fixed and biofilmed. The aerobic aeration is performed for 20 hours to obtain a primary aerobic treated garbage liquid. The amount of composite microbial agent 1 is calculated based on the weight of the food waste, and the inoculation amount is 1×10 8 cfu / kg.

[0118] (5) The primary aerobic treated sewage obtained in step (4) is pumped into the primary sedimentation tank, left to stand for 30 minutes, the supernatant overflows into the secondary aerobic tank, and the sediment is pumped back into the primary aerobic tank. (6) The overflowing supernatant of step (5) enters the secondary aerobic tank, aerobically aerated for 24 hours, and obtains the secondary aerobic treated garbage liquid. The secondary aerobic tank is pre-installed with a composite filler covering 70% of the effective volume, and the composite microbial agent II is pre-dosed, and the microorganisms have been fixed and biofilmed. The amount of composite microbial agent II added is based on the weight of the food waste, and the inoculation amount is 1×10 8 cfu / kg.

[0119] (7) The secondary aerobic treated garbage liquid obtained in step (6) is pumped into a secondary sedimentation tank and allowed to stand for 30 minutes. The supernatant overflows into the MBR tank for deep treatment, and the sediment is pumped back to the secondary aerobic tank.

[0120] (8) The treated liquid overflowing from the secondary sedimentation tank flows into the MBR tank, which is treated by the MBR membrane and finally obtains sewage that meets the discharge standards.

[0121] The COD, degradation rate, NH3-N, SS and pH value of the sewage before and after the treatment of food waste were tested, as shown in Table 1.

[0122] Table 1 Changes of various indicators before and after kitchen waste treatment

[0123]

[0124] As can be seen from the results, using the microbial agent-enzyme preparation composition and the method for treating kitchen waste provided by the present invention, after the primary aerobic treatment, the degradation rate of kitchen waste can reach 91%, and after the secondary aerobic treatment combined with the membrane bioreactor (MBR) treatment, the kitchen waste is further degraded, and the overall degradation rate reaches more than 97%, realizing the efficient degradation of high-concentration organic matter in the kitchen waste. The entire treatment process can be completed within 48 hours, and the COD of the garbage tail water is finally made to reach less than 500mg / L, meeting the discharge requirements, and significantly improving the degradation efficiency of kitchen waste. In addition, no matter how the garbage concentration changes, the indicators such as COD, NH-N, and SS of the treated garbage tail water remain stable, ensuring the consistency and stability of the tail water quality, and providing a reliable basis for further use in fertilizer preparation.

[0125] Taking into account the three factors of garbage treatment concentration, the amount of composite microbial agent plus composite enzyme preparation used, and the degradation rate, it is believed that Example 5 is the best treatment process, and the microbial agent-enzyme preparation composition provided in Example 2 is the best choice.

[0126] Comparative Example 1

[0127] This comparative example provides a method for quickly processing food waste. The steps, processes and parameters are the same as those in Example 5, except that the composite microbial agent used in step (4) is a liquid culture medium of Bacillus subtilis TRIIM1.01035, a liquid culture medium of Bacillus amyloliquefaciens TRIIM 1.00308 and a liquid culture medium of Lactobacillus fermentum TRIIM1.14005 in a mass ratio of 1:3:1:1, and a liquid culture medium of Candida columnaris TRIIM 2.10097.

[0128] The COD, degradation rate, NH3-N, SS and pH value of the sewage before and after the treatment of food waste were tested, as shown in Table 2.

[0129] Comparative Example 2

[0130] This comparative example provides a method for quickly treating food waste. The steps, process and parameters are the same as those in Example 5, except that the composite microbial agent used in step (4) is a liquid culture medium of Bacillus subtilis TRIIM1.01035, a liquid culture medium of Bacillus amyloliquefaciens TRIIM 1.00308 and a liquid culture medium of Candida columnaris TRIIM2.10097 in a mass ratio of 1:3:1.

[0131] The COD, degradation rate, NH3-N, SS and pH value of the sewage before and after the treatment of food waste were tested, as shown in Table 2.

[0132] Comparative Example 3

[0133] This comparative example provides a method for quickly processing food waste. The steps, process and parameters are the same as those in Example 5, except that the composite microbial agent used in step (6) is a liquid culture medium of Bacillus subtilis TRIIM 1.01077, a liquid culture medium of Bacillus licheniformis TRIIM 1.00418 and a liquid culture medium of Lactobacillus fermentum TRIIM 1.14010 in a mass ratio of 2:1:2.

[0134] The COD, degradation rate, NH3-N, SS and pH value of the sewage before and after the treatment of food waste were tested, as shown in Table 2.

[0135] Comparative Example 4

[0136] This comparative example provides a method for quickly processing food waste. The steps, process and parameters are the same as those in Example 5, except that the composite microbial agent used in step (6) is a liquid culture medium of Bacillus subtilis TRIIM1.01077, a liquid culture medium of Bacillus licheniformis TRIIM 1.00418 and a liquid culture medium of Candida columnaris TRIIM2.10102 in a mass ratio of 1:1:1.

[0137] The COD, degradation rate, NH3-N, SS and pH value of the sewage before and after the treatment of food waste were tested, as shown in Table 2.

[0138] Table 2 Changes of kitchen waste before and after treatment

[0139]

[0140] It can be seen from the experimental results of Example 5 and Comparative Examples 1 and 2 that the composite microbial agent 1 provided by the present invention exhibits excellent degradation effect in the primary aerobic treatment of food waste, with a degradation rate of 92.1%, which is 6.8% and 9.3% higher than the agent combinations of Comparative Examples 1 and 2, respectively. The high efficiency of the agent combination is due to the synergistic effect of Bacillus subtilis, Bacillus amyloliquefaciens and Lactobacillus fermentum, and the combination of the three efficiently degrades organic matter in the complex environment of the early stage of food waste treatment.

[0141] It can be seen from the experimental results of Example 5 and Comparative Examples 3 and 4 that the composite microbial agent 2 provided by the present invention shows a significant degradation advantage in the secondary aerobic treatment stage, and the degradation rate reaches 71.5%, which is significantly higher than the degradation effect of Comparative Examples 3 and 4. In the secondary aerobic treatment stage, the organic matter in the kitchen waste has been degraded from complex macromolecules to small and medium molecules, and the treatment focus is shifted to further decomposing these small and medium molecular substances and degrading difficult-to-decompose oils and cellulose. The bacterial agents used in the secondary aerobic treatment stage of Example 5 are Bacillus subtilis, Bacillus licheniformis, Lactobacillus fermentation and columnar Candida. The reasonable ratio and synergistic effect of these four bacteria significantly improve the degradation efficiency of organic matter. Bacillus licheniformis and Bacillus subtilis continuously and efficiently decompose organic matter, and the metabolites of columnar Candida and Lactobacillus fermentation, such as alcohol, carbon dioxide, and lactic acid, adjust the pH value of the environment, provide a suitable environment for microbial growth, inhibit miscellaneous bacteria, and promote the decomposition of oils and other difficult-to-degrade organic matter.

[0142] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A microbial agent-enzyme preparation composition for degrading food waste, characterized in that: The invention comprises a composite microbial agent 1, a composite microbial agent 2 and a composite enzyme preparation; the composite enzyme preparation comprises α-amylase, saccharifying enzyme, neutral protease, lipase and cellulase; the composite microbial agent 1 comprises Bacillus subtilis, Bacillus amyloliquefaciens and Lactobacillus fermentation; the composite microbial agent 2 comprises Bacillus subtilis, Bacillus licheniformis, Lactobacillus fermentation and Candida columnaris.

2. The microbial agent-enzyme preparation composition for degrading food waste according to claim 1, characterized in that: The mass ratio of the alpha-amylase, saccharifying enzyme, neutral protease, lipase and cellulase is 1-2:1-2:1-2:1-2:2-3.

3. The microbial agent-enzyme preparation composition for degrading food waste according to claim 2, characterized in that: The mass ratio of the alpha-amylase, saccharifying enzyme, neutral protease, lipase and cellulase is 2:1:1:1:

3.

4. The microbial agent-enzyme preparation composition for degrading food waste according to claim 1, characterized in that: The Bacillus subtilis in the composite microbial agent 1 is at least one of Bacillus subtilis TRIIM 1.01067, Bacillus subtilis TRIIM 1.01035, and Bacillus subtilis TRIIM 1.01075; the Bacillus amyloliquefaciens is at least one of Bacillus amyloliquefaciens TRIIM 1.00312, Bacillus amyloliquefaciens TRIIM 1.00308, and Bacillus amyloliquefaciens TRIIM 1.00324; the Lactobacillus fermentum is at least one of Lactobacillus fermentum TRIIM 1.14009, Lactobacillus fermentum TRIIM 1.14005, and Lactobacillus fermentum TRIIM 1.14003; and / or The Bacillus subtilis described in the composite microbial agent 2 is at least one of Bacillus subtilis TRIIM 1.01046, Bacillus subtilis TRIIM 1.01077, and Bacillus subtilis TRIIM 1.01080; the Bacillus licheniformis is at least one of Bacillus licheniformis TRIIM 1.00420, Bacillus licheniformis TRIIM 1.00418, and Bacillus licheniformis TRIIM1.00423; the Lactobacillus fermentum is at least one of Lactobacillus fermentum TRIIM 1.14021, Lactobacillus fermentum TRIIM1.14010, and Lactobacillus fermentum TRIIM 1.14026; the columnar Candida is at least one of columnar Candida TRIIM 2.10100, columnar Candida TRIIM 2.10102, and columnar Candida TRIIM 2.10108.

5. The microbial agent-enzyme preparation composition for degrading food waste according to claim 1, characterized in that: The composite microbial agent 1 and the composite microbial agent 2 further include biologically acceptable microbial agent carriers and / or auxiliary materials.

6. The microbial agent-enzyme preparation composition for degrading food waste according to claim 5, characterized in that: The carrier of the composite microbial agent 1 is a liquid culture medium; the composite microbial agent 1 is prepared by mixing the Bacillus subtilis, Bacillus amyloliquefaciens and Lactobacillus fermentum after enrichment culture according to the mass ratio of the liquid culture medium of 1-2:3-4:1-2; and / or The carrier used in the composite microbial agent 2 is a liquid culture medium; the composite microbial agent 2 is prepared by enriching and culturing the Bacillus subtilis, Bacillus licheniformis, Lactobacillus fermentum and Candida columnaris and mixing them according to the mass ratio of the liquid culture medium of 2-3:1-2:2-3:2-3.

7. The microbial agent-enzyme preparation composition for degrading food waste according to claim 6, characterized in that: The carrier of the composite microbial agent 1 is a liquid culture medium; the composite microbial agent 1 is prepared by mixing the Bacillus subtilis, Bacillus amyloliquefaciens and Lactobacillus fermentum in a mass ratio of 1:3:1 after enrichment culture; and / or The carrier used for the composite microbial agent 2 is a liquid culture medium; the composite microbial agent 2 is prepared by enriching and culturing the Bacillus subtilis, Bacillus licheniformis, Lactobacillus fermentum and Candida columnaris and mixing them in a mass ratio of 2:1:2:2 of the liquid culture medium.

8. Use of the microbial agent-enzyme preparation composition for degrading food waste according to any one of claims 1 to 7 in aerobic digestion treatment of food waste.

9. A method for rapidly processing food waste, characterized in that: The following steps are involved: S1: After crushing the kitchen waste, add water to dilute it, the material-water ratio is 1:4-5, and beat it until all the liquid passes through a 40-60 mesh sieve, and then send it to a hydrolysis tank for standby; S2: hydrolysis treatment: adjusting the organic matter concentration in the hydrolysis tank so that the COD is less than or equal to 15000 mg / L; adding the composite enzyme preparation in the microbial agent-enzyme preparation composition according to any one of claims 1 to 7 equivalent to 0.05% to 1% of the weight of the kitchen waste into the hydrolysis tank, starting stirring for enzymolysis, controlling the temperature at 25-40° C., stirring speed at 50-100 rpm, and treating for 1-3 hours to obtain a hydrolyzate; S3: primary aerobic: the hydrolyzate obtained in S2 is pumped into a primary aerobic tank and aerobically aerated for 20-24 hours to obtain a primary aerobic treated garbage liquid; the primary aerobic tank is pre-installed with a composite filler covering 60%-80% of the effective volume, and the composite microbial agent 1 in the microbial agent-enzyme preparation composition according to any one of claims 1 to 7 is pre-dosed, and the microorganisms are fixed and biofilmed; the access amount of the composite microbial agent 1 is a viable bacterial count greater than or equal to 1×10 6 cfu / kg; S4: primary sedimentation: the garbage liquid obtained from the primary aerobic treatment in S3 is pumped into the primary sedimentation tank, left to stand for 20-40 minutes, the supernatant overflows into the secondary aerobic tank, and the sediment is pumped back to the primary aerobic tank; S5: Secondary aerobic: The supernatant from the overflow of S4 enters the secondary aerobic tank and is aerobically aerated for 20-24 hours to obtain the garbage liquid treated by secondary aerobic treatment; the secondary aerobic tank is pre-installed with a composite filler covering 60%-80% of the effective volume, and the composite microbial agent II in the microbial agent-enzyme preparation composition according to any one of claims 1 to 7 is pre-dosed, and the microorganisms are fixed and biofilmed; the access amount of the composite microbial agent II is calculated based on the weight of the kitchen waste, and the number of viable bacteria is greater than or equal to 1×10 6 cfu / kg; S6: Secondary sedimentation: The garbage liquid obtained from the secondary aerobic treatment in S5 is pumped into the secondary sedimentation tank, left to stand for 20-40 minutes, the supernatant overflows into the MBR tank for deep treatment, and the sediment is pumped back to the secondary aerobic tank; S7: MBR deep treatment: The supernatant from the overflow of the secondary sedimentation tank into the MBR tank is treated by the MBR membrane to obtain sewage that meets the discharge standards.

10. A method for rapidly processing food waste according to claim 9, characterized in that: The material-water ratio in S1 is 1:4.5; the composite enzyme preparation equivalent to 0.6% of the weight of the kitchen waste is added into the hydrolysis tank in S2, the temperature is controlled at 35°C, the stirring speed is 100 rpm, and the treatment time is 3 hours; the access amount of the composite microbial agent 1 in S3 is the number of live bacteria greater than or equal to 1×10 8 cfu / kg; the access amount of the composite microbial agent II in S5 is that the number of viable bacteria is greater than or equal to 1×10 8 cfu / kg.

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