High-yield bamboo-source lactic acid fermentation method

Abstract

According to the technical scheme, the high-yield bamboo-source lactic acid fermentation method is characterized in that a raw material treatment assembly, an enzymolysis treatment assembly, a fermentation treatment assembly, a separation and purification assembly and a lactic acid collection assembly are included; the extraction process is divided into five steps of raw material treatment, enzymolysis, fermentation, separation and purification and lactic acid collection, so that the whole extraction process is clearer and more efficient, meanwhile, by optimizing operation parameters and equipment design of each component, especially in the stages of raw material treatment, enzymolysis and fermentation, the production cycle can be shortened, the yield of lactic acid can be increased, and the production cost can be reduced. According to the present invention, through the solid-liquid separator, the filter, the evaporation concentrator and other devices, the impurities and the solid residues can be effectively removed, the purity of the lactic acid product can be improved, the problems of the traditional process can be overcome, the production efficiency, the product quality and the market competitiveness can be improved, and the production cost can be reduced. And the method has a positive promotion effect on the development of the bamboo lactic acid industry.

Description

Technical Field

[0001] The invention relates to the technical field of lactic acid extraction, and in particular to a high-yield bamboo-derived lactic acid fermentation method. Background Art

[0002] Bamboo lactic acid refers to lactic acid extracted from bamboo by biochemical methods. Lactic acid is an organic acid widely found in nature, and its molecular formula is C3H6O3. As a renewable biomass resource, bamboo is rich in cellulose, hemicellulose and lignin, which can be converted into lactic acid through proper treatment and fermentation.

[0003] However, the traditional bamboo-derived lactic acid extraction process usually involves multiple steps, so the production cycle is long and the output is limited, which cannot meet the rapid growth of market demand. In addition, the bamboo-derived lactic acid products extracted by traditional processes have low purity and may contain impurities or other components, affecting the quality and market competitiveness of the product. To solve the above problems, we proposed a high-yield bamboo-derived lactic acid fermentation method. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a high-yield bamboo-derived lactic acid fermentation method to solve the problems raised in the background technology.

[0005] The above technical objectives of the present invention are achieved through the following technical solutions:

[0006] A high-yield bamboo-derived lactic acid fermentation method, comprising: a raw material processing component 100, an enzymatic hydrolysis processing component 200, a fermentation processing component 300, a separation and purification component 400 and a lactic acid collection component 500;

[0007] The raw material processing assembly 100 includes: a crusher, a steam machine;

[0008] The crusher is used to crush the bamboo into small pieces or fibers to increase the specific surface area for subsequent processing, wherein the crushing size is 2-5 mm, and then the steam machine is used to process, soften and decompose the lignin and cellulose in the bamboo.

[0009] Preferably, the pretreatment temperature of the steam machine is 160-180°C for 30-60 minutes, and then a dilute acid (such as sulfuric acid, concentration 0.5-1%) is used for chemical pretreatment at a temperature of 120-140°C for 1-2 hours.

[0010] Preferably, the enzymatic hydrolysis treatment component 200: an enzymatic hydrolysis reactor;

[0011] The enzymatic hydrolysis reactor comprises: a pH meter, a temperature sensor, and an automatic acid / alkali adding device, which are used to monitor and adjust parameters such as temperature and pH value to ensure the efficiency of enzymatic hydrolysis. Cellulase and hemicellulase can be added during use to decompose cellulose and hemicellulose into fermentable sugars, wherein the enzyme concentration is 10-30 units / gram of dry bamboo, the temperature is 45-55°C, the pH value is maintained at 4.8-5.5, and the time is 24-72 hours.

[0012] Preferably, the fermentation processing assembly 300 includes: a fermentation tank, a mixer;

[0013] The fermentation tank is used to ferment the enzymatically hydrolyzed sugars and convert them into lactic acid. The stirrer is used to ensure the uniformity of the fermentation process, wherein the stirring speed is 50-150 rpm and the temperature is maintained at 30-37°C during the process.

[0014] Preferably, the separation and purification component 400 comprises: a solid-liquid separator, a filter and an evaporation concentrator;

[0015] The solid-liquid separator is a filter press and a spiral separator, which is used to separate solid residues in the fermentation liquid, and then further purify the lactic acid solution through the filter, wherein the filter pore size is 0.1-0.2 microns.

[0016] Preferably, the evaporation concentrator is used to concentrate the lactic acid solution, requiring a temperature of 50-70° C. and performing low-temperature vacuum evaporation.

[0017] Preferably, the lactic acid collection assembly 500 comprises: a storage tank, a packaging machine;

[0018] The collected lactic acid is placed in the storage tank, and then bottled, bottled or in other forms using the packaging machine for filling and sealing.

[0019] The present invention also provides a high-yield bamboo-derived lactic acid fermentation method, which comprises the following steps:

[0020] S1, putting bamboo into the bamboo crusher to crush it into small pieces or fibers of 2-5 mm;

[0021] S2, the crushed bamboo is placed in a steam machine, steam is injected, maintained at 160-180°C, and treated for 30-60 minutes, and then the bamboo is placed in a treatment tank, diluted acid (sulfuric acid concentration 0.5-1%) is added, heated to 120-140°C, and maintained for 1-2 hours for pretreatment;

[0022] S3, transferring the pretreated bamboo to the enzymatic hydrolysis reactor, adding cellulase and hemicellulase, the enzyme concentration is 10-30 units / gram of dry bamboo, adjusting the temperature to 45-55°C, the pH value to 4.8-5.5, and the enzymatic hydrolysis reaction continues for 24-72 hours;

[0023] S4, transferring the enzymatically hydrolyzed sugar solution to the fermentation tank, turning on the stirrer and adjusting the stirring speed to 50-150 rpm, maintaining the fermentation temperature at 30-37° C., and fermenting until the sugar is converted into lactic acid;

[0024] S5, sending the fermented mixed solution into the solid-liquid separator, using a filter press and a spiral separator to separate the solid residue, and then using a filter (pore size 0.1-0.2 microns) to further purify the lactic acid solution;

[0025] S6, sending the purified lactic acid solution into the evaporation concentrator for low-temperature vacuum evaporation, maintaining the temperature at 50-70° C. to concentrate the lactic acid solution;

[0026] S7, filling and sealing the collected lactic acid solution through the packaging machine.

[0027] In summary, the present invention mainly has the following beneficial effects:

[0028] The invention divides the extraction process into five steps of raw material treatment, enzymolysis, fermentation, separation and purification, and lactic acid collection, so that the whole extraction process can be made clearer and more efficient. Meanwhile, by optimizing the operating parameters and equipment design of each component, especially in the raw material treatment, enzymolysis and fermentation stages, the production cycle can be shortened, the output of lactic acid can be increased, and the rapid growth of market demand can be met. Moreover, through equipment such as a solid-liquid separator, a filter, and an evaporation concentrator, impurities and solid residues can be effectively removed, and the purity of the lactic acid product can be increased. Thus, many problems of the traditional process are overcome, the production efficiency, product quality and market competitiveness are improved, and the development of the bamboo source lactic acid industry can be actively promoted. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the structure of a bamboo-derived lactic acid extraction device;

[0030] Figure 2 The present invention is a flow chart of a high-yield bamboo-derived lactic acid fermentation method.

[0031] Reference numerals: 100: raw material processing component; 200: enzymatic hydrolysis processing component; 300: fermentation processing component; 400: separation and purification component; 500: lactic acid collection component. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings of the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0033] The following examples are used to illustrate the present invention, but they cannot be used to limit the scope of protection of the present invention. The conditions in the examples can be further adjusted according to specific conditions. Simple improvements to the method of the present invention under the premise of the concept of the present invention belong to the scope of protection claimed in the present invention.

[0034] refer to Figure 1-Figure 2 , a bamboo-derived lactic acid extraction device, comprising: a raw material processing component 100, an enzymatic hydrolysis processing component 200, a fermentation processing component 300, a separation and purification component 400 and a lactic acid collection component 500;

[0035] The raw material processing assembly 100 includes: a crusher, a steam machine;

[0036] The crusher is used to crush the bamboo into small pieces or fibers to increase the specific surface area for subsequent processing, wherein the crushing size is 2-5 mm, and then the steam machine is used to process, soften and decompose the lignin and cellulose in the bamboo.

[0037] refer to Figure 1-Figure 2 The pretreatment temperature of the steam machine is 160-180°C for 30-60 minutes, and then a dilute acid (such as sulfuric acid, concentration 0.5-1%) is used for chemical pretreatment at a temperature of 120-140°C for 1-2 hours. The enzymatic treatment component 200: enzymatic reactor;

[0038] The enzymatic hydrolysis reactor comprises: a pH meter, a temperature sensor, and an automatic acid / alkali adding device, which are used to monitor and adjust parameters such as temperature and pH value to ensure the efficiency of enzymatic hydrolysis. Cellulase and hemicellulase can be added during use to decompose cellulose and hemicellulose into fermentable sugars, wherein the enzyme concentration is 10-30 units / gram of dry bamboo, the temperature is 45-55°C, the pH value is maintained at 4.8-5.5, and the time is 24-72 hours.

[0039] refer to Figure 1-Figure 2 , the fermentation processing assembly 300 includes: a fermentation tank, a mixer;

[0040] The fermentation tank is used to ferment the enzymatically hydrolyzed sugars and convert them into lactic acid. The stirrer is used to ensure the uniformity of the fermentation process, wherein the stirring speed is 50-150 rpm and the temperature is maintained at 30-37° C. during the process. The separation and purification component 400 includes: a solid-liquid separator, a filter and an evaporation concentrator;

[0041] The solid-liquid separator is a filter press and a spiral separator, which are used to separate solid residues in the fermentation liquid, and then the lactic acid solution is further purified by the filter, wherein the filter pore size is 0.1-0.2 microns. The evaporation concentrator is used to concentrate the lactic acid solution, requiring a temperature of 50-70°C and low-temperature vacuum evaporation.

[0042] refer to Figure 1-Figure 2 , the lactic acid collection assembly 500 includes: a storage tank, a packaging machine;

[0043] The collected lactic acid is placed in the storage tank, and then bottled, bottled or in other forms using the packaging machine for filling and sealing.

[0044] The present invention also provides a high-yield bamboo-derived lactic acid fermentation method, which comprises the following steps:

[0045] S1, putting bamboo into the bamboo crusher to crush it into small pieces or fibers of 2-5 mm;

[0046] S2, the crushed bamboo is placed in a steam machine, steam is injected, maintained at 160-180°C, and treated for 30-60 minutes, and then the bamboo is placed in a treatment tank, diluted acid (sulfuric acid concentration 0.5-1%) is added, heated to 120-140°C, and maintained for 1-2 hours for pretreatment;

[0047] S3, transferring the pretreated bamboo to the enzymatic hydrolysis reactor, adding cellulase and hemicellulase, the enzyme concentration is 10-30 units / gram of dry bamboo, adjusting the temperature to 45-55°C, the pH value to 4.8-5.5, and the enzymatic hydrolysis reaction continues for 24-72 hours;

[0048] S4, transferring the enzymatically hydrolyzed sugar solution to the fermentation tank, turning on the stirrer and adjusting the stirring speed to 50-150 rpm, maintaining the fermentation temperature at 30-37° C., and fermenting until the sugar is converted into lactic acid;

[0049] S5, sending the fermented mixed solution into the solid-liquid separator, using a filter press and a spiral separator to separate the solid residue, and then using a filter (pore size 0.1-0.2 microns) to further purify the lactic acid solution;

[0050] S6, sending the purified lactic acid solution into the evaporation concentrator for low-temperature vacuum evaporation, maintaining the temperature at 50-70° C. to concentrate the lactic acid solution;

[0051] S7, filling and sealing the collected lactic acid solution through the packaging machine.

[0052] Working principle: Please refer to Figure 1-Figure 2 As shown, the present invention divides the extraction process into five steps of raw material treatment, enzymolysis, fermentation, separation and purification, and lactic acid collection, which can make the entire extraction process clearer and more efficient. At the same time, by optimizing the operating parameters and equipment design of each component, especially in the raw material treatment, enzymolysis and fermentation stages, the production cycle can be shortened, the output of lactic acid can be increased, and the rapid growth of market demand can be met. In addition, through equipment such as solid-liquid separators, filters, and evaporation concentrators, impurities and solid residues can be effectively removed, and the purity of the lactic acid product can be improved. Many problems of traditional processes have been overcome, production efficiency, product quality and market competitiveness have been improved, and the development of the bamboo source lactic acid industry has a positive role in promoting.

[0053] Although the embodiments of the present invention have been shown and described, it is understandable to those skilled in the art that, unless otherwise defined, the technical terms or scientific terms used in the present invention shall have the usual meanings understood by persons with ordinary skills in the field to which the present invention belongs. The words "including" or "comprising" and the like used in the present invention mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. The words "connect" or "connected" and the like are not limited to physical or mechanical connections, but may also include electrical connections, whether direct or indirect. "Up", "down", "left", "right", etc. are only used to indicate relative position relationships. When the absolute position of the described object changes, the relative position relationship may also change accordingly.

[0054] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A high-yield bamboo-derived lactic acid fermentation method, characterized in that: include: A raw material processing component 100, an enzymatic processing component 200, a fermentation processing component 300, a separation and purification component 400 and a lactic acid collection component 500; The raw material processing assembly 100 includes: a crusher, a steam machine; The crusher is used to crush the bamboo into small pieces or fibers to increase the specific surface area for subsequent processing, wherein the crushing size is 2-5 mm, and then the steam machine is used to process, soften and decompose the lignin and cellulose in the bamboo.

2. A high-yield bamboo-derived lactic acid fermentation method according to claim 1, characterized in that: The pretreatment temperature of the steam machine is 160-180° C. for 30-60 minutes, and then a dilute acid (such as sulfuric acid, concentration 0.5-1%) is used for chemical pretreatment at a temperature of 120-140° C. for 1-2 hours.

3. A high-yield bamboo-derived lactic acid fermentation method according to claim 1, characterized in that: The enzymatic hydrolysis treatment component 200: an enzymatic hydrolysis reactor; The enzymatic hydrolysis reactor comprises: a pH meter, a temperature sensor, and an automatic acid / alkali adding device, which are used to monitor and adjust parameters such as temperature and pH value to ensure the efficiency of enzymatic hydrolysis. Cellulase and hemicellulase can be added during use to decompose cellulose and hemicellulose into fermentable sugars, wherein the enzyme concentration is 10-30 units / gram of dry bamboo, the temperature is 45-55°C, the pH value is maintained at 4.8-5.5, and the time is 24-72 hours.

4. A high-yield bamboo-derived lactic acid fermentation method according to claim 1, characterized in that: The fermentation processing assembly 300 includes: a fermentation tank and a mixer; The fermentation tank is used to ferment the enzymatically hydrolyzed sugars and convert them into lactic acid. The stirrer is used to ensure the uniformity of the fermentation process, wherein the stirring speed is 50-150 rpm and the temperature is maintained at 30-37°C during the process.

5. The high-yield bamboo-derived lactic acid fermentation method according to claim 1, characterized in that: The separation and purification component 400 includes: a solid-liquid separator, a filter and an evaporation concentrator; The solid-liquid separator is a filter press and a spiral separator, which is used to separate solid residues in the fermentation liquid, and then further purify the lactic acid solution through the filter, wherein the filter pore size is 0.1-0.2 microns.

6. A high-yield bamboo-derived lactic acid fermentation method according to claim 5, characterized in that: The evaporation concentrator is used to concentrate the lactic acid solution, requires a temperature of 50-70° C., and performs low-temperature vacuum evaporation.

7. A high-yield bamboo-derived lactic acid fermentation method according to claim 1, characterized in that: The lactic acid collection assembly 500 includes: a storage tank and a packaging machine; The collected lactic acid is placed in the storage tank, and then bottled, bottled or in other forms using the packaging machine for filling and sealing.

8. A high-yield bamboo-derived lactic acid fermentation method, characterized in that: Using the bamboo-derived lactic acid extraction device according to any one of claims 1 to 7, the method comprises the following steps: S1, putting bamboo into the bamboo crusher to crush it into small pieces or fibers of 2-5 mm; S2, the crushed bamboo is placed in a steam machine, steam is injected, maintained at 160-180°C, and treated for 30-60 minutes, and then the bamboo is placed in a treatment tank, diluted acid (sulfuric acid concentration 0.5-1%) is added, heated to 120-140°C, and maintained for 1-2 hours for pretreatment; S3, transferring the pretreated bamboo to the enzymatic hydrolysis reactor, adding cellulase and hemicellulase, the enzyme concentration is 10-30 units / gram of dry bamboo, adjusting the temperature to 45-55°C, the pH value to 4.8-5.5, and the enzymatic hydrolysis reaction continues for 24-72 hours; S4, transferring the enzymatically hydrolyzed sugar solution to the fermentation tank, turning on the stirrer and adjusting the stirring speed to 50-150 rpm, maintaining the fermentation temperature at 30-37° C., and fermenting until the sugar is converted into lactic acid; S5, sending the fermented mixed solution into the solid-liquid separator, using a filter press and a spiral separator to separate the solid residue, and then using a filter (pore size 0.1-0.2 microns) to further purify the lactic acid solution; S6, sending the purified lactic acid solution into the evaporation concentrator for low-temperature vacuum evaporation, maintaining the temperature at 50-70° C. to concentrate the lactic acid solution; S7, filling and sealing the collected lactic acid solution through the packaging machine.

Images