Blood aggregation parameter detector

Abstract

The present application discloses a blood aggregation parameter detector. In this blood aggregation parameter detector, when the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the capacity of the first depolymerization module is greater than or equal to 5 and less than or equal to 50; or, when the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the capacity of the first depolymerization module is greater than or equal to 10 and less than or equal to 100. Based on the above method, the reliability of the blood aggregation parameter detector can be improved.

Description

Technical Field

[0001] This application relates to the technical field of sample detection, and particularly to a blood aggregation parameter detector. Background Art

[0002] Blood contains various types of particles, such as exosomes, hemoglobin, platelets, red blood cells, white blood cells, etc., and each type of particle also contains various sub-types. Due to the diversity of chemical substances and physical states on various particles, it can be regarded as a suspension under a multi-phase physical field. In a relatively static state, various (or sub-types) of cells often aggregate together, and the different aggregation parameters of various blood cells often reveal different pathological states.

[0003] However, during the blood detection process, multiple blood samples (usually corresponding to multiple patients) are often collected at different times, locations, and methods, and then collected together for batch detection. Their relatively static time, state, and influencing factors are different; and the order of batch detection further severely damages the environmental factor consistency between different samples when detecting the aggregation parameters of batch samples.

[0004] Existing blood aggregation parameter detectors can usually only inhale blood samples for aggregation parameter detection. When detecting different batches of batch samples, even if they are the same blood sample, due to the different relatively static time, state, and influencing factors, the obtained results of the aggregation parameter detection will be different. That is, the detection results of existing blood aggregation parameter detectors are prone to distortion and have poor reliability. Summary of the Invention

[0005] The main technical problem to be solved by this application is how to improve the reliability of a blood aggregation parameter detector.

[0006] To solve the above technical problems, the technical solution adopted in this application is as follows: A blood aggregation parameter detector, comprising: a sample preparation module for preparing a blood sample to be detected; a first depolymerization module, the first end of the first depolymerization module is connected to the sample preparation module; a first pipeline module, the second end of the first depolymerization module is connected to the first end of the first pipeline module; a second depolymerization module, the second end of the first pipeline module is connected to the first end of the second depolymerization module; a second pipeline module, the second end of the second depolymerization module is connected to the first end of the second pipeline module; a first depolymerization module, the second end of the second pipeline module is connected to the first end of the first depolymerization module; a third pipeline module; a second depolymerization module, the second depolymerization module is connected to the first end or the second end of the first depolymerization module through the third pipeline module; an aggregation parameter detection module, the aggregation parameter detection module is arranged on the first pipeline module or the second pipeline module, and the aggregation parameter detection module is used to detect the aggregation parameters of the blood sample in the first pipeline module or the second pipeline module; when the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the capacity of the first depolymerization module is greater than or equal to 5 and less than or equal to 50, or, when the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the capacity of the first depolymerization module is greater than or equal to 10 and less than or equal to 100.

[0007] Wherein, when the first depolymerization module and / or the second depolymerization module aspirate and discharge the blood sample, the maximum displacement of one end of the blood sample is greater than 1 / 2 of the length of the inner cavity of the sample preparation module, and / or, the blood sample passes through the first pipeline module, and / or, the blood sample does not pass through the second depolymerization module.

[0008] Wherein, the opening direction of one end of the first depolymerization module is different from the opening direction of the other end of the first depolymerization module; and / or, the opening direction of one end of the second depolymerization module is different from the opening direction of the other end of the second depolymerization module; and / or, when the blood sample passes through the first depolymerization module, it undergoes at least one 180-degree turn; and / or, when the blood sample passes through the second depolymerization module, it undergoes at least one 180-degree turn; and / or, the minimum pipe diameter of the second depolymerization module is different from the pipe diameter of the first pipeline module; and / or, the minimum pipe diameter of the second depolymerization module is different from the pipe diameter of the second pipeline module; and / or, the maximum pipe diameter of the second depolymerization module is different from the pipe diameter of the first pipeline module; and / or, the maximum pipe diameter of the second depolymerization module is different from the pipe diameter of the second pipeline module; and / or, the pipe length of the first pipeline module is greater than or equal to 5 cm.

[0009] Among them, the extending direction of the inner cavity of the sample preparation module is parallel to the extending direction of the inner cavity of the first pipeline module, and the distance between the projection of the sample preparation module on the horizontal plane and the projection of the first pipeline module on the horizontal plane is less than 6 cm; and / or, the distance between the projection of one end of the first depolymerization module on the horizontal plane and the projection of the other end of the first depolymerization module on the horizontal plane is less than 6 cm.

[0010] Among them, the first pipeline module includes a first sub-pipeline module and a second sub-pipeline module connected to each other; both ends of the first sub-pipeline module are relatively fixed, keeping the axis of the inner cavity of the first sub-pipeline module as a straight line perpendicular to the horizontal plane; one end of the second sub-pipeline module is connected to one end of the first sub-pipeline module, and the other end of the second sub-pipeline module is connected to the second depolymerization module. The second sub-pipeline module can undergo corresponding deformation and / or movement when relative movement occurs between one end and the other end of the second sub-pipeline module.

[0011] Among them, when the polymerization parameter detection module detects the polymerization parameters, the distances among the polymerization parameter detection module, the first sub-pipeline module, and the sample preparation module remain unchanged; and / or; when the polymerization parameter detection module detects the polymerization parameters, keep the first depolymerization module stationary and keep the sample preparation module stationary in the vertical direction; and / or; when the polymerization parameter detection module detects the polymerization parameters, keep the polymerization parameter detection module, the first pipeline module, and the sample preparation module stationary.

[0012] Among them, the blood polymerization parameter detector further includes: a first joint module, which is respectively connected to the first depolymerization module and the first sub-pipeline module; a second joint module, which is respectively connected to the first sub-pipeline module and the second sub-pipeline module; the pipe material parameters between any two adjacent modules among the first joint module, the first sub-pipeline module, the second joint module, and the second sub-pipeline module are different, and / or, the pipe material parameters of the first depolymerization module and the first sub-pipeline module are different, and / or, the pipe material parameters of the first sub-pipeline module and the second sub-pipeline module are different; the pipe material parameters include at least one of the inner wall roughness, material, shape, and pipe diameter of the pipe.

[0013] Among them, when the module with a larger capacity in the first depolymerization module and the second depolymerization module aspirates and discharges the blood sample for depolymerization, the module with a smaller capacity in the first depolymerization module and the second depolymerization module also aspirates and discharges the blood sample for depolymerization.

[0014] Among them, when both the first depolymerization module and the second depolymerization module aspirate and discharge the blood sample for depolymerization, the depolymerization speed of the module with a smaller capacity in the first depolymerization module and the second depolymerization module is greater than the depolymerization speed of the module with a larger capacity in the first depolymerization module and the second depolymerization module; and / or, the driving speed of the first depolymerization module during blood separation is less than the driving speed of the first depolymerization module during depolymerization.

[0015] Wherein, the first depolymerization module and / or the second depolymerization module are configured to: before the blood sample reaches the polymerization parameter detection module, the first depolymerization module and / or the second depolymerization module aspirate and expel the blood sample for depolymerization.

[0016] Wherein, the capacity of the first depolymerization module is less than or equal to three times the maximum blood sample volume required for detections other than polymerization parameter detection in a single sample detection, or the capacity of the first depolymerization module is greater than the maximum optical detection sample volume required for detections other than polymerization parameter detection in a single sample detection; and / or, the ratio of the capacity of the second depolymerization module to the capacity of the first depolymerization module is greater than or equal to 40 and less than or equal to 120.

[0017] Wherein, the blood polymerization parameter detector further includes a reaction cell module; when the sample preparation module pushes a sample to the reaction cell module, the first depolymerization module must perform a pushing operation, and the second depolymerization module must not perform a pushing operation, and / or, when the sample preparation module pushes a microliter non-sample liquid to the reaction cell module, the first depolymerization module must perform a pushing operation, and / or, when the sample preparation module aspirates a microliter non-sample liquid, the first depolymerization module must perform an aspiration operation, and / or, when the sample preparation module pushes a milliliter non-sample liquid to the reaction cell module, the second depolymerization module must perform a pushing operation, and / or, when the sample preparation module aspirates a liquid, the second depolymerization module must perform an aspiration operation.

[0018] Wherein, the blood polymerization parameter detector further includes at least two reaction cell modules. During the process of pushing a milliliter non-sample liquid to the reaction cell modules through the first pipeline module and the sample preparation module in sequence, the collected milliliter non-sample liquid is first injected into the reaction cell module containing a hemolytic agent among the at least two reaction cell modules.

[0019] Wherein, when the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the driving power of the second depolymerization module during aspiration or pushing operations is the first power; when the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the driving power of the second depolymerization module during aspiration or pushing operations is the second power; the first power is less than the second power.

[0020] The beneficial effects of the present application are as follows: Different from the prior art, in the technical solution of the present application, the first end of the first depolymerization module is connected to the sample preparation module, the second end of the first depolymerization module is connected to the first end of the first pipeline module, the second end of the first pipeline module is connected to the first end of the second depolymerization module, the second end of the second depolymerization module is connected to the first end of the second pipeline module, the second end of the second pipeline module is connected to the first end of the first depolymerization module, the second depolymerization module is connected to the first end or the second end of the first depolymerization module through the third pipeline module, and the polymerization parameter detection module is arranged on the first pipeline module or the second pipeline module. Among them, when the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 μL, the capacity of the first depolymerization module is greater than 80 μL, and the ratio of the capacity of the second depolymerization module to the capacity of the first depolymerization module is greater than or equal to 5 and less than or equal to 50. Or, when the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 μL, the capacity of the first depolymerization module is greater than 80 μL, and the ratio of the capacity of the second depolymerization module to the capacity of the first depolymerization module is greater than or equal to 10 and less than or equal to 100. Based on the above method, the blood sample can be depolymerized and depolymerized by each depolymerization module and each depolymerization module to reduce or eliminate the influence caused by the relative rest of the blood sample, improve the accuracy of the subsequent detection of the polymerization parameters based on the polymerization parameter detection module, and improve the reliability of the blood polymerization parameter detector. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0022] Figure 1 It is one of the schematic structural diagrams of an embodiment of the blood polymerization parameter detector of the present application;

[0023] Figure 2 It is the second of the schematic structural diagrams of an embodiment of the blood polymerization parameter detector of the present application;

[0024] Figure 3 It is the schematic structural diagram of the whole machine of the blood polymerization parameter detector of the present application;

[0025] Figure 4 It is the schematic structural diagram of an embodiment of the second depolymerization module of the present application.

[0026] Reference numerals: 1, sample preparation module; 2, first depolymerization module; 3, first pipeline module; 4, second depolymerization module; 41, first inlet and outlet; 42, second inlet and outlet; 5, second pipeline module; 6, first depolymerization module; 7, third pipeline module; 8, second depolymerization module; 9, polymerization parameter detection module; 10, three-way valve; 11, cleaning swab. Detailed implementation manners

[0027] The present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be specifically noted that the following embodiments are only used to illustrate the present application, but do not limit the scope of the present application. Similarly, the following embodiments are only partial embodiments of the present application rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present application.

[0028] The mention of "embodiment" in this article means that the specific features, structures or characteristics described in connection with the embodiment may be included in at least one embodiment of the present application. The phrase appears in various positions in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0029] In the description of the present application, it should be noted that unless otherwise clearly specified and limited, the terms "installation", "setting", "connection", and "coupling" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be a direct connection or an indirect connection through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0030] The present application provides a blood polymerization parameter detector. Refer to Figures 1 to 3 , Figure 1 which is one of the structural schematic diagrams of an embodiment of the blood polymerization parameter detector of the present application, Figure 2 which is another structural schematic diagram of an embodiment of the blood polymerization parameter detector of the present application, Figure 3 which is the overall structural schematic diagram of the blood polymerization parameter detector of the present application. As shown in Figure 1 and Figure 2 , the blood polymerization parameter detector includes a sample preparation module 1, a first depolymerization module 2, a first pipeline module 3, a second depolymerization module 4, a second pipeline module 5, a first depolymerization module 6, a third pipeline module 7, a second depolymerization module 8, and a polymerization parameter detection module 9.

[0031] The sample preparation module 1 is used to prepare a blood sample to be tested, wherein the sample preparation module 1 may be an infusion needle or a sampling needle, and the sample preparation module 1 may be provided with corresponding power to inhale or spit out the blood sample.

[0032] The first end of the first deagglomeration module 2 is connected to the sample preparation module 1. The first deagglomeration module 2 can be a non-straight pipeline with a large bend, for example, Figure 1 The first deagglomeration module 2 of the U-shaped pipeline shown, for example Figure 2 The first deagglomeration module 2 of the S-shaped pipeline shown. Since the pipeline has a certain degree of roughness and the large curved section causes the flow direction of the blood sample to change rapidly, the curved pipeline affects the flow velocity and direction of the blood sample inside and the push and pull of the driving device, which can cause irregular small-scale turbulence in the local part of the pipeline, thereby breaking up, stirring, impacting, and rotating the aggregated particles, thereby achieving deagglomeration treatment.

[0033] like Figure 3 As shown, the sample preparation module 1 can be used to move along the direction of its slide rail on the track A. If the first deagglomeration module 2 connected to the upper end of the sample preparation module 1 is a U-shaped pipeline, at least part of the first pipeline module 3 connected to the first deagglomeration module 2 will extend from top to bottom, and the remaining part of the first pipeline module 3 or other pipelines connected to the first pipeline module 3 can be placed in the drag chain B, and the drag chain B can be located below the guide rail A. If the first deagglomeration module 2 connected to the upper end of the sample preparation module 1 is an S-shaped pipeline, at least part of the first pipeline module 3 connected to the first deagglomeration module 2 will extend from bottom to top, and the remaining part of the first pipeline module 3 or other pipelines connected to the first pipeline module 3 can be placed in the drag chain B, and the drag chain B can be located above the guide rail A.

[0034] The second end of the first deagglomeration module 2 is connected to the first end of the first pipeline module 3. The first deagglomeration module 2 and the first pipeline module 3 may be different parts of the same pipeline, or different pipelines made of different materials.

[0035] The second end of the first pipeline module 3 is connected to the first end of the second deagglomeration module 4. Specifically, the second deagglomeration module 4 may be a two-way valve, the inner diameter of which when turned on is different from the inner diameter of the first pipeline module 3 and the inner diameter of the second pipeline module 5, respectively. When the liquid passes through the pipeline and the two-way valve, due to the influence caused by the different inner diameters and the different local flow directions, irregular small-scale turbulence may be generated locally, thereby having the effects of breaking up, stirring, impacting, rotating, shearing, etc. on the polymerized particles, thereby achieving deagglomeration treatment.

[0036] The second end of the second depolymerization module 4 is connected to the first end of the second pipeline module 5. The second end of the second pipeline module 5 is connected to the first end of the first depolymerization module 6. Among them, the first depolymerization module 6 can be connected to the second end of the second depolymerization module 4 through the second pipeline module 5. The first depolymerization module 6 is used for sucking and discharging liquid, so that the blood sample in the pipeline connected to the first depolymerization module 6 moves back and forth along the pipeline extension direction, further strengthening the scale and intensity of the locally generated irregular small-scale turbulence, and further strengthening the effects of breaking up, stirring, impacting, rotating, shearing, etc. on the aggregated particles, thereby realizing the depolymerization treatment.

[0037] The second depolymerization module 8 is connected to the first end or the second end of the first depolymerization module 6 through the third pipeline module 7. Among them, the second depolymerization module 8 can be connected to the first end or the second end of the first depolymerization module 6 through the third pipeline module 7, so that the second depolymerization module 8 can jointly suck and discharge liquid with the first depolymerization module 6, so that the blood sample in the pipeline connected to it moves back and forth along the pipeline extension direction, further strengthening the scale and intensity of the locally generated irregular small-scale turbulence, and further strengthening the effects of breaking up, stirring, impacting, rotating, shearing, etc. on the aggregated particles, thereby realizing a further depolymerization treatment. In addition, only one of the depolymerization modules can also be used for sucking and discharging liquid to realize the depolymerization treatment, which can be determined according to actual needs and is not limited here.

[0038] The polymerization parameter detection module 9 is arranged on the first pipeline module 3 or the second pipeline module 5. The polymerization parameter detection module 9 is used for detecting the polymerization parameters of the blood sample in the first pipeline module 3 or the second pipeline module 5.

[0039] Here, the polymerization parameters can refer to the polymerization parameters of various substances in the blood, including, for example: Proportion of Exosome Aggregation, Hemoglobin Sedimentation Ratio, Erythrocyte Sedimentation Rate, etc.

[0040] Among them, in one example (not shown in the figure), when the second depolymerization module 8 is connected to the first end of the first depolymerization module 6 through the third pipeline module 7, that is, when the second depolymerization module 8 and the first depolymerization module 6 are based on the same pipeline as the opening and jointly suck and discharge liquid, the sum of the capacities of the first depolymerization module 2 and the first pipeline module 3 is greater than 150 microliters, the capacity of the first depolymerization module 6 is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module 8 to the capacity of the first depolymerization module 6 is greater than or equal to 5 and less than or equal to 50.

[0041] Or, in another example, such as Figure 1 and Figure 2As shown, when the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, that is, when the second depolymerization module 8 is connected to the first depolymerization module 6 to indirectly connect to the first pipeline module 3, the sum of the capacities of the first depolymerization module 2 and the first pipeline module 3 is greater than 150 microliters, the capacity of the first depolymerization module 6 is greater than 80 microliters, and the capacity ratio of the second depolymerization module 8 to the first depolymerization module 6 is greater than or equal to 10 and less than or equal to 100.

[0042] In the above two examples, when the second depolymerization module 8 and the first depolymerization module 6 are based on the same pipeline as the opening and jointly perform liquid suction and exhalation, the second depolymerization module 8 and the first depolymerization module 6 can be made to superimpose their suction and exhalation power at their openings, so that only one of the depolymerization modules can be controlled to perform suction and exhalation when not needed, and both depolymerization modules can be controlled to perform suction and exhalation when needed; the driving control time can be carried out simultaneously or in time-sharing, and the time periods of the driving control of the two can also overlap partially, depending on the specific needs; and the direction of the driving control (suction or exhalation, push or draw) and the size of the motor driving current are superimposed to meet more depolymerization scene requirements. When the second depolymerization module 8 is connected to the first depolymerization module 6 to indirectly connect the first pipeline module 3, since the second depolymerization module 8 and the first depolymerization module 6 are in a progressive connection relationship, and since the pipe resistance of the series mode is relatively greater than the pipe resistance of the parallel mode, the value range of the ratio of the capacity of the second depolymerization module 8 to the first depolymerization module 6 can be increased, so that the two depolymerization modules in the two examples can have the ability to provide depolymerization processing with basically the same or basically similar intensity range.

[0043] In practice, the capacity of the second depolymerization module 8 is greater than that of the first depolymerization module 6. The blood sample can be collected by the sample preparation module 1 based on the first depolymerization module 6 only, and the blood sample does not pass through the second depolymerization module 4. The blood sample is collected by the depolymerization module with a smaller capacity, which can effectively improve the accuracy of sampling. When necessary, the two depolymerization modules with a smaller capacity and a larger capacity can also be controlled to collect blood samples at the same time, which can effectively improve the collection efficiency of blood samples, which is not limited here.

[0044] After completing the collection of the blood sample, the second depolymerization module 8 and the first depolymerization module 6 can be controlled to perform suction and exhalation together to depolymerize the blood sample, thereby improving the efficiency of the depolymerization process, and after the depolymerization process, the polymerization parameters of the blood sample are detected based on the polymerization parameter detection module 9, thereby reducing or eliminating the influence of different degrees of particle aggregation caused by relative stillness of blood samples for polymerization parameter detection in different batches, thereby improving the accuracy and consistency of the blood polymerization parameter detector for detecting polymerization parameters of blood samples from different batches, thereby improving the reliability of the blood polymerization parameter detector.

[0045] It should be noted that the blood aggregation parameter detector can be used to detect routine blood tests, CRP (C-reactive protein), erythrocyte sedimentation rate, and other parameters of blood samples. After collecting the blood samples and detecting the depolymerization, depolymerization, and aggregation parameters, they can be transported to the corresponding reaction pools to obtain the detection results of the corresponding parameters. In actual detection, the sample volume required for detection is usually no more than 160 μL of blood sample. Among them, the sample volume required for detecting the erythrocyte sedimentation rate is usually no more than 140 μL, and the sample volume required for routine blood tests is usually no more than 35 μL.

[0046] The sum of the capacities of the first pipeline module 3 and the second pipeline module 5 is greater than or equal to 250 μL, and the capacity of the sample preparation module 1 is greater than or equal to 15 μL and less than or equal to 30 μL, so that the first pipeline module 3, the second pipeline module 5, and the sample preparation module 1 can accommodate the blood samples required for each detection, and can even accommodate the total blood samples required for multiple detections at the same time.

[0047] The sum of the lengths of the first pipeline module 3 and the second pipeline module 5 can be greater than or equal to 500 mm, and the inner pipe diameter is less than or equal to 2 mm.

[0048] In order to obtain the reasonable parameters mentioned in our above technical solution, we borrowed the hydrodynamic model constructed in the paper Pomerenka O, Carrillo Segura S, Cao F, Wu J, Ristroph L. Hydrodynamics of finite-length pipes at intermediate Reynolds numbers[J]. Journal of Fluid Mechanics, 2023, 959: A28. to analyze the above fluid characteristics. The organizational framework of the model proposed in the paper is obtained by dimensional analysis of the smooth circular pipe flow problem. The relevant physical parameters include the pipe length L and inner diameter D, fluid density ρ and viscosity μ, average flow velocity U, and pressure difference Δp. These six quantities can be regarded as one dependent variable and five independent variables, such as . The specific model formula is as follows:

[0049]

[0050] For the critical Reynolds number , many existing laws are not applicable. The influence of the development of turbulent flow has been proven not to be limited to a relatively short entrance region, and its length It is relatively sensitive to Re, and the above model can accurately calculate fluid friction, pressure-flow relationships, and other characteristics. Among them, the six parameters and their values are respectively = 0.85, Re* = 2000, δ = 200, b∞ = 1, k = 0.05, and = 0.04. The pipe diameter is the upper limit of the inner pipe diameter of the first pipeline module 3 or the second pipeline module 5, which is 2 mm. At present, the overall detection speed of the cell parameter analyzer is getting faster and faster, resulting in the flow velocity in the pipeline, especially the local flow velocity, may exceed 20 m / s. Then, there is a high probability that the Reynolds number of the fluid flow state in the pipeline will fall into the critical range of the Reynolds number . Then, substitute other empirical parameters into the above formula, and multiply the sum of the lengths of the first pipeline module 3 and the second pipeline module 5 obtained by calculation by a certain redundancy coefficient (selected as 3 according to experience). Then, the sum of the lengths of the first pipeline module 3 and the second pipeline module 5 can be obtained as 0.4931 m. After appropriate rounding, the sum of the lengths of the first pipeline module 3 and the second pipeline module 5 should be greater than or equal to 500 mm. The minimum pipe diameter of the first pipeline module 3 and the second pipeline module 5 is generally 0.8 mm (if the pipe diameter is thinner, the pipe resistance will be too large, which may bring greater negative impacts). Then, the corresponding capacity sum is 251.2 ul, and after rounding, it is 250 ul.

[0051] Different from the prior art, in the technical solution of the present application, the first end of the first depolymerization module 2 is connected to the sample preparation module 1, the second end of the first depolymerization module 2 is connected to the first end of the first pipeline module 3, the second end of the first pipeline module 3 is connected to the first end of the second depolymerization module 4, the second end of the second depolymerization module 4 is connected to the first end of the second pipeline module 5, the second end of the second pipeline module 5 is connected to the first end of the first depolymerization module 6, and the second depolymerization module 8 is connected to the first end or the second end of the first depolymerization module 6 through the third pipeline module 7. The polymerization parameter detection module 9 is disposed on the first pipeline module 3 or the second pipeline module 5. Among them, when the second depolymerization module 8 is connected to the first end of the first depolymerization module 6 through the third pipeline module 7, the sum of the capacities of the first depolymerization module 2 and the first pipeline module 3 is greater than 150 μL, the capacity of the first depolymerization module 6 is greater than 80 μL, and the ratio of the capacity of the second depolymerization module 8 to the capacity of the first depolymerization module 6 is greater than or equal to 5 and less than or equal to 50. Or, when the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, the sum of the capacities of the first depolymerization module 2 and the first pipeline module 3 is greater than 150 μL, the capacity of the first depolymerization module 6 is greater than 80 μL, and the ratio of the capacity of the second depolymerization module 8 to the capacity of the first depolymerization module 6 is greater than or equal to 10 and less than or equal to 100. The first depolymerization module 6 and the second depolymerization module 8 can be plunger pumps. The plunger pump is composed of a rigid outer shell, a wear-resistant piston, and a driving mechanism. Such a pump forms a seal by relying on the contact surface between the piston and the outer shell. The amount of liquid added by such a syringe / pump is determined by the stroke of the piston movement and the inner cross-sectional area of the outer shell at this stroke. Due to processing tolerances, there are generally differences in the inner cross-sectional area of the outer shell in different regions of the stroke. Generally, when the second depolymerization module 8 is connected to the first end of the first depolymerization module 6 through the third pipeline module 7, the system pipe resistance for the second depolymerization module 8 to drive the suction and discharge action is smaller, while when the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, the system pipe resistance for the second depolymerization module 8 to drive the suction and discharge action is larger. According to the parameters such as the model, capacity, power, driving current, and static current of the commonly used plunger pump, the parameters of the first depolymerization module 6 and the second depolymerization module 8 can be determined to be different.

[0052] Based on the above method, the blood sample can be depolymerized and depolymerized by each depolymerization module and each depolymerization module first, so as to reduce or eliminate the influence of particle aggregation caused by the relative rest of the blood sample, improve the accuracy of subsequent detection of the polymerization parameters based on the polymerization parameter detection module 9, and improve the reliability of the blood polymerization parameter detector.

[0053] In one embodiment, when the first depolymerization module 6 and / or the second depolymerization module 8 sucks and discharges the blood sample:

[0054] The maximum displacement at one end of the blood sample is greater than 1 / 2 of the length of the inner cavity of the sample preparation module 1.

[0055] And / or, the blood sample passes through the first pipeline module 3.

[0056] And / or, the blood sample does not pass through the second depolymerization module 4.

[0057] Specifically, when controlling the first depolymerization module 6 and / or the second depolymerization module 8 to aspirate and discharge the blood sample, so as to aspirate or discharge the blood sample through the sample preparation module 1, realizing the depolymerization and repolymerization of the blood sample, and the subsequent collection and injection processes, the maximum displacement of one end of the blood sample starting from entering the sample preparation module 1 is greater than 1 / 2 of the length of the inner cavity of the sample preparation module 1, which not only reduces the possibility that the collected blood sample is insufficient or overflows from the sample preparation module 1 during aspiration and discharge, but also increases the length of the pipeline through which the sample flows during aspiration and discharge, which is equivalent to increasing the roughness of the pipeline wall to reduce the time and strength of particle aggregation, enhancing the depolymerization effect, thereby further improving the reliability of the blood polymerization parameter detector.

[0058] In addition, after the blood sample enters the sample preparation module 1, the blood sample passes through the first pipeline module 3, but does not pass through the second depolymerization module 4, so as to realize the detection of the polymerization parameters of the blood sample by the polymerization parameter detection module 9 provided on the first pipeline module 3. Since the internal pipeline shape of the second depolymerization module 4 is irregular, the pipe diameter changes greatly, and there may be some dead ends, which are difficult to clean, it can also reduce the possibility that the blood sample causes pollution to the second depolymerization module 4 and is difficult to clean, thereby improving the reliability of the blood polymerization parameter detector.

[0059] In one embodiment, the opening direction of one end of the first depolymerization module 2 is different from the opening direction of the other end of the first depolymerization module 2.

[0060] And / or, the opening direction of one end of the second depolymerization module 4 is different from the opening direction of the other end of the second depolymerization module 4.

[0061] And / or, when the blood sample passes through the first depolymerization module 2, it undergoes at least one 180-degree turn.

[0062] And / or, when the blood sample passes through the second depolymerization module 4, it undergoes at least one 180-degree turn.

[0063] And / or, the minimum pipe diameter of the second depolymerization module 4 is different from the pipe diameter of the first pipeline module 3.

[0064] And / or, the minimum pipe diameter of the second depolymerization module 4 is different from the pipe diameter of the second pipeline module 5.

[0065] And / or, the maximum pipe diameter of the second depolymerization module 4 is different from the pipe diameter of the first pipeline module 3.

[0066] And / or, the maximum pipe diameter of the second depolymerization module 4 is different from the pipe diameter of the second pipeline module 5.

[0067] And / or, the pipe length of the first pipeline module 3 is greater than or equal to 5 cm.

[0068] Specifically, first, by making the opening direction at one end of the first depolymerization module 2 different from the opening direction at the other end of the first depolymerization module 2, and / or by causing the blood sample to undergo at least one 180-degree turn when passing through the first depolymerization module 2, it is possible to make the orientation of the blood sample different between the first inlet / outlet and the second inlet / outlet of the first depolymerization module 2, or to change the flow direction multiple times, enhancing the disturbance ability of the first depolymerization module 2 to the blood sample. By causing the blood sample to undergo at least one inversion process, the scale and intensity of the locally generated irregular small-scale turbulence are further enhanced, and the effects of breaking up, agitating, impacting, rotating, and shearing the aggregated particles are further strengthened. Using the gravitational force, the aggregation influence therein is reduced. The first depolymerization module 2 such as the U-shaped pipeline or the S-shaped pipeline described above can all achieve at least one inversion process to achieve one-time depolymerization.

[0069] Similarly, referring to Figure 4 , Figure 4 is a schematic structural diagram of an embodiment of the second depolymerization module 4 of the present application. As Figure 4 shown, by making the opening direction at one end of the second depolymerization module 4 different from the opening direction at the other end of the second depolymerization module 4, and / or by causing the blood sample to undergo at least one 180-degree turn when passing through the second depolymerization module 4, it is possible to make the orientation of the blood sample different between the first inlet / outlet 41 and the second inlet / outlet 42 of the second depolymerization module 4, or to change the flow direction at least once, enhancing the disturbance ability of the second depolymerization module 4 to the blood sample, further enhancing the scale and intensity of the locally generated irregular small-scale turbulence, and further strengthening the effects of breaking up, agitating, impacting, rotating, and shearing the aggregated particles. By causing the blood sample to undergo at least one inversion process, the aggregation influence therein is reduced using the gravitational force. Here, the minimum pipe diameter of the second depolymerization module 4 can be the minimum through diameter of the valve.

[0070] Second, by making the minimum pipe diameter of the second deflocculation module 4 different from that of the first pipeline module 3, and / or the minimum pipe diameter of the second deflocculation module 4 different from that of the second pipeline module 5, and / or the maximum pipe diameter of the second deflocculation module 4 different from that of the first pipeline module 3, and / or the maximum pipe diameter of the second deflocculation module 4 different from that of the second pipeline module 5, it is possible to further strengthen the scale and intensity of the locally generated irregular small-scale turbulence after the blood sample passes through any two devices with different pipe diameters above, due to the difference in the pipe diameter inside the pipeline where it flows, further strengthening the effects of breaking up, agitating, impacting, rotating, shearing, etc. on the aggregated particles, realizing further perturbation of the blood sample, and thus realizing further deflocculation treatment.

[0071] Third, when the polymerization parameter detection module 9 is arranged on the first pipeline module 3, due to the diameter change treatment of the blood sample between the first pipeline module 3 and the second deflocculation module 4, or in other words, the second deflocculation module 4 is a variable-diameter deflocculation module, and there is a direction change treatment of the blood sample between the first pipeline module 3 and the first deflocculation module 2, or in other words, the first deflocculation module 2 is a direction-changing deflocculation module, the scale and intensity of the locally generated irregular small-scale turbulence are respectively strengthened, and the effects of breaking up, agitating, impacting, rotating, shearing, etc. on the aggregated particles are further strengthened. And this way of respectively arranging the diameter change treatment and the direction change treatment on both sides of the first pipeline module 3 further strengthens the scale and intensity of the locally generated irregular small-scale turbulence, further strengthens the effects of breaking up, agitating, impacting, rotating, shearing, etc. on the aggregated particles, and can further strengthen the effect of the deflocculation treatment.

[0072] Moreover, based on the difference between the maximum and minimum sedimentation rates of the blood sample and the flow rate of the blood sample, it can be determined that on the first pipeline module 3 provided with the polymerization parameter detection module 9 for detecting the polymerization parameters, there needs to be a sufficient sedimentation distance to improve the accuracy of the detection of the polymerization parameters. Therefore, by making the pipe length of the first pipeline module 3 greater than or equal to 5 cm, the reliability of the blood polymerization parameter detector can be improved.

[0073] It should be noted that the pipe length of the first pipeline module 3 is the pipeline length of the first pipeline module 3 itself, or the distance between the fixing structures (such as cable ties) respectively located at both ends of the first pipeline module 3 used to fix the first pipeline module 3, or the pipeline length of at least part of the first pipeline module 3 wrapped by the polymerization parameter detection module 9 that can prevent stray light from entering, or the pipeline length of at least part of the first pipeline module 3 wrapped by the polymerization parameter detection module 9 for heating the pipeline at the wrapping position.

[0074] In one embodiment, the extending direction of the inner cavity of the sample preparation module 1 is parallel to the extending direction of the inner cavity of the first pipeline module 3, and the distance between the projection of the sample preparation module 1 on the horizontal plane and the projection of the first pipeline module 3 on the horizontal plane is less than 6 cm. It should be noted that all projections are ideally a point or a circle. However, regardless of the projection shape or the pipeline shape in the three-dimensional space, as long as there are two projections on the horizontal plane, it does not affect the limitation of the distance between the projections on the horizontal plane.

[0075] And / or, the distance between the projection of one end of the first depolymerization module 2 on the horizontal plane and the projection of the other end of the first depolymerization module 2 on the horizontal plane is less than 6 cm.

[0076] Specifically, both ends of the first pipeline module 3 can be fixed to the corresponding plate members. For example, the two ends of the first pipeline module 3 can be fixed by cable ties, so that the connection line between the two ends of the first pipeline module 3 can be arranged parallel to the axis of the needle inner cavity of the sample preparation module 1 serving as the sampling needle. This needle inner cavity axis is the axis of the inner cavity of the needle body of the sample preparation module 1 except for the needle head.

[0077] By making the distance between the projection of the sample preparation module 1 on the horizontal plane and the projection of the first pipeline module 3 on the horizontal plane less than 6 cm, or making the distance between the projection of one end of the first depolymerization module 2 on the horizontal plane and the projection of the other end of the first depolymerization module 2 on the horizontal plane less than 6 cm, it can make the non-linear pipeline described above between the sample preparation module 1 and the first pipeline module 2 have a higher bending degree, that is, a greater degree of curvature change, further strengthening the scale and intensity of the irregular small-scale turbulence generated locally, and further strengthening the effects of dispersing, stirring, impacting, rotating, shearing, etc. on the aggregated particles, and improving the depolymerization treatment effect of the first depolymerization module 2.

[0078] In one embodiment, the first pipeline module 3 includes a first sub-pipeline module and a second sub-pipeline module that are connected to each other.

[0079] Both ends of the first sub-pipeline module are relatively fixed, keeping the axis of the inner cavity of the first sub-pipeline module as a straight line perpendicular to the horizontal plane.

[0080] One end of the second sub-pipeline module is connected to one end of the first sub-pipeline module, and the other end of the second sub-pipeline module is connected to the second depolymerization module 4. The second sub-pipeline module can undergo corresponding deformation and / or movement when relative movement occurs between one end and the other end of the second sub-pipeline module.

[0081] Specifically, both ends of the first sub-pipeline module can be fixed to the corresponding plate members. For example, the two ends of the first sub-pipeline module can be fixed by cable ties, such that the inner cavity axis of the pipeline portion between the two ends of the first sub-pipeline module is a straight line perpendicular to the horizontal plane. One end of the second sub-pipeline module is connected to one end of the first sub-pipeline module, thereby realizing the fixation of this section of the second sub-pipeline module. The unfixed other end of the second sub-pipeline module can pass through the aforementioned drag chain B and be connected to the second depolymerization module 4. Based on the above method, through the method of fixation and the drag chain B, the pipeline shaking of the blood sample during the depolymerization and repolymerization processes can be reduced, thereby reducing the inconsistency between blood samples caused by the turbulence brought about by the pipeline shaking during the depolymerization and repolymerization processes, improving the consistency of the detection of different batches of blood samples, and thus improving the reliability of the blood polymerization parameter detector.

[0082] Optionally, when the polymerization parameter detection module 9 detects the polymerization parameters, the distances among the polymerization parameter detection module 9, the first sub-pipeline module, and the sample preparation module 1 remain unchanged.

[0083] And / or, when the polymerization parameter detection module 9 detects the polymerization parameters, keep the first depolymerization module 2 stationary and keep the sample preparation module 1 stationary in the vertical direction.

[0084] And / or, when the polymerization parameter detection module 9 detects the polymerization parameters, keep the polymerization parameter detection module 9, the first pipeline module 3, and the sample preparation module 1 stationary.

[0085] Specifically, by making the distances among the polymerization parameter detection module 9, the first sub-pipeline module, and the sample preparation module 1 remain unchanged when the polymerization parameter detection module detects the polymerization parameters, and / or keeping the first depolymerization module 2 stationary and keeping the sample preparation module 1 stationary in the vertical direction, and / or keeping the polymerization parameter detection module 9, the first pipeline module 3, and the sample preparation module 1 stationary, it is possible to reduce the pipeline shaking of the blood sample in the first pipeline module detected by the polymerization parameter detection module 9, so that the blood sample can settle normally, that is, by making the first sub-pipeline module itself stable enough, the accuracy of the detection by the polymerization parameter detection module 9 can be improved.

[0086] Furthermore, the blood polymerization parameter detector further includes a first joint module and a second joint module.

[0087] The first joint module is respectively connected to the first depolymerization module 2 and the first sub-pipeline module.

[0088] The second joint module is respectively connected to the first sub-pipeline module and the second sub-pipeline module.

[0089] The pipe material parameters are different between any two adjacent modules among the first joint module, the first sub-pipeline module, the second joint module, and the second sub-pipeline module, and / or the pipe material parameters of the first depolymerization module 2 and the first sub-pipeline module are different, and / or the pipe material parameters of the first sub-pipeline module and the second sub-pipeline module are different. The pipe material parameters include at least one of the inner wall roughness, material, shape, and pipe diameter of the pipe.

[0090] Specifically, the first sub-pipeline module can specifically be a glass tube. For example, it can be a glass tube with a square outer diameter. The polymerization parameter detection module 9 can be arranged outside the glass tube serving as the first sub-pipeline module to detect the polymerization parameters of the blood sample inside through the glass tube. The first joint module and the second joint module adjacent to the first sub-pipeline module respectively can be pipelines of other material types that are not glass tubes, and there is no limitation here.

[0091] Furthermore, the first sub-pipeline module can be a glass tube with a square outer diameter and a circular inner diameter. The first joint module and the second joint module adjacent to the first sub-pipeline module respectively can be pipelines of other material types that are not glass tubes. The inner diameters of the first joint module and the second joint module can be exactly the same as that of the first sub-pipeline module respectively, and the connection at the pipe joint is smoothly transitioned, reducing the possibility of local turbulence caused by the non-smooth shape or pipe diameter change at the transition, thereby improving the accuracy of the polymerization parameter detection module 9 for detecting the polymerization parameters of the blood sample. Similarly, the second sub-pipeline module can also specifically be a glass tube. For example, it can be a glass tube with a square outer diameter and a circular inner diameter. The polymerization parameter detection module 9 can be arranged outside the glass tube serving as the second sub-pipeline module to detect the polymerization parameters of the blood sample inside through the glass tube. The second joint module adjacent to the second sub-pipeline module can be a pipeline of other material types that are not glass tubes, and there is no limitation here.

[0092] Optionally, the pipeline material of the first sub-pipeline module is at least one of polyether ether ketone, polytetrafluoroethylene, polyethylene, and polypropylene, which can enable the light beam generated by the polymerization parameter detection module 9 to enter the first sub-pipeline module to detect the cell vertical parameters of the sample, improving the measurement accuracy of the polymerization parameter detection module 9.

[0093] In one embodiment, the light transmittance of the first sub-pipeline module is greater than or equal to 90%, and the haze is less than or equal to 5%, enabling almost all the light beams emitted by the polymerization parameter detection module 9 to enter the first sub-pipeline module for detection, improving the measurement accuracy of the polymerization parameter detection module 9.

[0094] Integrating the method of arranging the variable-diameter depolymerization module and the variable-direction depolymerization module on both sides of the first pipeline module 3 as described above further enhances the scale and intensity of the irregular small-scale turbulence generated locally, further strengthens the effects of dispersing, agitating, impacting, rotating, and shearing the polymerized particles, and can further enhance the effect of the depolymerization treatment.

[0095] Meanwhile, at the detection location of the polymerization parameter detection module 9, a more transparent and easier-to-clean material is used to ensure the accuracy of the polymerization parameter detection of the blood sample. When the blood sample is transported in the glass tube, due to the low roughness of the inner wall of the glass tube, the relatively stable material, the relatively flat shape, and the appropriate tube diameter, the stability of the blood sample during transportation in the glass tube can be effectively improved, thereby enhancing the accuracy of the polymerization parameter detection module 9 in detecting the polymerization parameters of the blood sample through the glass tube.

[0096] In addition, the first joint module and the second joint module can be provided with chamfers at the connection, which can reduce the possibility of blood sample residue at this connection.

[0097] In one embodiment, when the module with a larger capacity in the first depolymerization module 6 and the second depolymerization module 8 aspirates and discharges the blood sample for depolymerization, the module with a smaller capacity in the first depolymerization module 6 and the second depolymerization module 8 also aspirates and discharges the blood sample for depolymerization.

[0098] Specifically, it can be based only on the first depolymerization module 6 aspirating and discharging the blood sample for depolymerization, or it can be based on both the first depolymerization module 6 and the second depolymerization module 8 aspirating and discharging the blood sample for depolymerization. When it is necessary to use the module with a larger capacity for depolymerization, usually a greater depolymerization force is required. Therefore, the first depolymerization module 6 and the second depolymerization module 8 can be controlled to jointly aspirate and discharge the blood sample for depolymerization to improve the depolymerization efficiency. When using the depolymerization module with a smaller capacity for depolymerization, a smaller depolymerization force may be required. Therefore, only this one depolymerization module can be controlled for depolymerization, or both depolymerization modules can be controlled for depolymerization, depending on specific needs.

[0099] Based on the above method, the rationality of the depolymerization force when controlling the depolymerization module for depolymerization can be improved, thereby enhancing the rationality and reliability of the depolymerization.

[0100] Optionally, when both the first depolymerization module 6 and the second depolymerization module 8 aspirate and discharge the blood sample for depolymerization, the depolymerization speed of the module with a smaller capacity in the first depolymerization module 6 and the second depolymerization module 8 is greater than the depolymerization speed of the module with a larger capacity in the first depolymerization module 6 and the second depolymerization module 8;

[0101] and / or, the driving speed of the first depolymerization module 6 during blood distribution is less than the driving speed of the first depolymerization module 6 during depolymerization.

[0102] Specifically, the depolymerization rate can specifically be the volume of the liquid transported per unit time by the corresponding depolymerization module during the extraction operation or the pushing operation. For example, when the depolymerization module is a syringe, the depolymerization rate can also be understood as the product value of the number of syringe push-pull steps per unit time of the corresponding syringe and the volume corresponding to a single step of the syringe.

[0103] When the first depolymerization module 6 and the second depolymerization module 8 both aspirate and expel the blood sample for depolymerization, the depolymerization rate of the module with the smaller volume among the first depolymerization module 6 and the second depolymerization module 8 is greater than the depolymerization rate of the module with the larger volume among the first depolymerization module 6 and the second depolymerization module 8, which can make the amounts of the extraction operation or the pushing operation per unit time of the first depolymerization module 6 and the second depolymerization module 8 relatively close, so as to achieve the stability when depolymerizing the blood sample synchronously, thereby improving the reliability of depolymerization.

[0104] By making the driving speed of the first depolymerization module 6 during blood distribution smaller than the driving speed of the first depolymerization module 6 during depolymerization, the accuracy during blood distribution can be relatively high, while the efficiency during depolymerization can be relatively high. Since blood distribution pays more attention to accuracy and depolymerization pays more attention to efficiency, based on this method, the rationality of the control of the depolymerization module during blood distribution and depolymerization can be improved synchronously, and further the reliability during blood distribution and depolymerization can be improved.

[0105] In one embodiment, the first depolymerization module 6 and / or the second depolymerization module 8 is used for: before the blood sample reaches the polymerization parameter detection module 9, the first depolymerization module 6 and / or the second depolymerization module 8 aspirates and expels the blood sample for depolymerization.

[0106] Specifically, depolymerization can be carried out before the blood sample reaches the polymerization parameter detection module 9, so that the blood sample located between the pipeline where the polymerization parameter detection module 9 is located and the blood sample inlet end of the sample preparation module 1 is affected by the first depolymerization module 6 and / or the second depolymerization module 8 to complete depolymerization, and then the polymerization parameter detection module 9 completes the detection of the polymerization parameters of the depolymerized blood sample, improving the accuracy of the detection.

[0107] In one embodiment, as Figure 1 shown, the blood polymerization parameter detector includes a three-way valve 10.

[0108] The common end of the three-way valve 10 is connected to the second depolymerization module 8, the first branch port of the three-way valve 10 is connected to the first depolymerization module 6, and the second branch port of the three-way valve 10 is connected to the container storing the diluent.

[0109] Specifically, when the liquid in the pipeline is insufficient but depolymerization is required, the second depolymerization module 8 can be controlled to suck the diluent from the container storing the diluent, so as to fill the sucked diluent into the pipeline with insufficient liquid, thereby ensuring the smooth progress of the depolymerization process. The pipeline filled with the diluent can, in combination with the suction and discharge of the second depolymerization module 8, provide the power for the depolymerization treatment of the blood sample, improving the reliability of the blood polymerization parameter detector.

[0110] In one embodiment, the capacity of the first depolymerization module 6 is less than or equal to three times the maximum blood sample volume required for detections other than the polymerization parameter detection in a single sample detection. For example, if the maximum blood sample volume required for detections other than the polymerization parameter detection in a single sample detection is 45 μl, the capacity of the first depolymerization module 6 can be 100 μl. As mentioned before, the smaller the capacity of the depolymerization module, the generally corresponding improvement in the accuracy of suction and discharge, thereby improving the measurement accuracy of the whole machine.

[0111] Specifically, in one example, the blood sample volume required for detections other than the polymerization parameter detection in a single sample detection can usually be 40 microliters, of which 35 microliters can be used for blood routine detection, and the capacity of the first depolymerization module 6 is less than or equal to 120 microliters. The first depolymerization module 6 in this example can be used for the sub-sampling operation of the collected blood sample. In addition, the blood sample volume required for the polymerization parameter detection is usually 140 microliters. Usually, a blood sample with a volume of the sum of 140 microliters and 40 microliters is inhaled, and a total of 20 microliters of blood samples at the head end and the tail end are discarded, and 160 microliters of blood samples are used for the polymerization parameter detection respectively, as well as the sub-sampling operation of blood routine detection or other detections.

[0112] In another example, the capacity of the first depolymerization module 6 can be less than or equal to 250 microliters, for example, it can be 250 microliters. The first depolymerization module 6 in this example can be used for the sub-sampling operation of the collected blood sample and as the driving source during the flow-type sheath fluid auxiliary propulsion, which is not limited here.

[0113] Specifically, in one embodiment, the blood polymerization parameter detector further includes a reaction cell module. The capacity of the first depolymerization module 6 is greater than the maximum optical detection sample volume required for detections other than polymerization parameter detection in a single sample detection. Here, the optical detection sample volume refers to the liquid obtained after adding a sample and a reagent into the reaction cell module for reaction, and after being pushed and pulled, the liquid volume fed into the optical detection module (such as an optical flow cell) for measurement. For example, if the maximum optical detection sample volume required for detections other than polymerization parameter detection in a single sample detection is 215 ul, the capacity of the first depolymerization module 6 can be 250 ul. Since the first depolymerization module 6 performs other tasks in addition to depolymerization, it can better complete other tasks while ensuring a relatively high degree of suction and discharge accuracy. Moreover, when the optical detection module (such as an optical flow cell) performs measurements, it has extremely high requirements for the stability of the liquid path. When the first depolymerization module performs a one-way push, it can ensure the corresponding liquid path stability. However, if a back-drawing process is involved in the middle, the return difference of its components is sufficient to destroy the liquid path stability. Therefore, the measurement by the optical detection module (such as an optical flow cell) must be completed by a one-way push. Therefore, the capacity of 6 is greater than the maximum optical detection sample volume required for detections other than polymerization parameter detection in a single sample detection.

[0114] In another embodiment, the ratio of the capacity of the second depolymerization module 8 to that of the first depolymerization module 6 is greater than or equal to 40 and less than or equal to 120. For example, if the capacity of the first depolymerization module 6 is 100 ul or 250 ul, and the capacity of the second depolymerization module 8 is 10 ml, the capacity ratios are 100 times or 40 times respectively. The second depolymerization module 8 is used for extraction operations to collect a blood sample through the sample preparation module 1, and the first depolymerization module 6 is used for pushing operations to sub-sample the blood sample through the sample preparation module 1.

[0115] By using the second depolymerization module 8 for extraction operations to collect a blood sample through the sample preparation module 1, due to the relatively large capacity of the second depolymerization module 8, the efficiency of the collection operation can be effectively improved.

[0116] By using the first depolymerization module 6 for pushing operations to sub-sample the blood sample through the sample preparation module 1, due to the relatively small capacity of the first depolymerization module 6, the accuracy of the sub-sampling operation can be effectively improved.

[0117] In one embodiment, the blood polymerization parameter detector further includes a reaction cell module. The reaction cell module can accommodate a blood sample and related reaction reagents, and they react with each other to obtain a reacted sample. A part of the reacted sample can be sent to an optical detection module (such as an optical flow cell) for measurement. The optical detection sample volume is the volume of the liquid after adding the sample and reaction reagents in the reaction cell module and reacting, and after pushing and pulling, the volume of the liquid sent to the optical detection module (such as an optical flow cell) for measurement. The optical detection sample volume is less than the sample volume of the reacted sample. The reacted sample that is not sent to the optical detection module (such as an optical flow cell) for measurement can be discharged as waste liquid into the waste liquid tank. In one embodiment, when the sample preparation module 1 pushes the sample to the reaction cell module, the first depolymerization module 6 must perform a pushing operation, and the second depolymerization module 8 must not perform a pushing operation; the smaller the capacity of the depolymerization module, and generally the corresponding accuracy of suction and discharge will also be improved, thereby improving the measurement accuracy of the whole machine. Since the capacity of the second depolymerization module 8 is much larger than that of the first depolymerization module 6, the first depolymerization module 6 distributes the sample more precisely, thereby improving the measurement accuracy of the whole machine.

[0118] In one embodiment, when the sample preparation module 1 pushes microliter non-sample liquid to the reaction cell module, the first depolymerization module 6 must perform a pushing operation; here the microliter non-sample liquid can be the dye solution in the reaction reagent, and the dosage is generally only about 20 μl, and the maximum does not exceed 100 μl, and the price is expensive. Since the capacity of the second depolymerization module 8 is much larger than that of the first depolymerization module 6, the first depolymerization module 6 distributes the microliter non-sample liquid more precisely, which not only reduces the waste caused by the inaccuracy of the microliter non-sample liquid, but also improves the measurement accuracy of the whole machine.

[0119] In one embodiment, when the sample preparation module 1 extracts microliter non-sample liquid, the first depolymerization module 6 must perform an extraction operation; since the capacity of the second depolymerization module 8 is much larger than that of the first depolymerization module 6, the first depolymerization module 6 extracts the microliter non-sample liquid more precisely, which not only reduces the waste caused by the inaccuracy of the microliter non-sample liquid, but also improves the measurement accuracy of the whole machine.

[0120] In one embodiment, when the sample preparation module 1 pushes milliliter non-sample liquid to the reaction cell module, the second depolymerization module 8 must perform a pushing operation; since the capacity of the second depolymerization module 8 is much larger than that of the first depolymerization module 6, the driving and pushing ability is stronger, and the cleaning efficiency is higher, thereby reducing the corrosion of the pipeline. The milliliter non-sample liquid here can be the probe liquid sucked inside or outside the machine, and the dosage generally exceeds 100 μl. When using the probe liquid, usually a relatively large amount is required, and more places need to be cleaned. For example, wherever there is a blood sample, there may be residues of blood proteins, and all need to be cleaned with the probe liquid.

[0121] In one embodiment, when the liquid is extracted by the sample preparation module 1, the second depolymerization module 8 must perform the extraction operation; since the capacity of the second depolymerization module 8 is much larger than that of the first depolymerization module 6, the driving suction capacity is stronger and the extraction efficiency is higher. The liquid here includes the sample (liquid) and milliliter-level non-sample liquid. When extracting the milliliter-level non-sample liquid, the extraction speed is fast, reducing the residence time of the milliliter-level non-sample liquid in the pipeline, thereby reducing the corrosion of the pipeline; when extracting the sample, since the capacity of the second depolymerization module 8 is much larger than that of the first depolymerization module 6, the ability to drive and suction the sample is stronger, the sample can be collected faster, and the speed and efficiency of the whole machine are increased. Specifically, in one example, the blood polymerization parameter detector further includes at least 2 reaction cell modules. Among them, in the process of pushing the milliliter-level non-sample liquid to the reaction cell modules through the first pipeline module 3 and the sample preparation module 1 in sequence, the collected milliliter-level non-sample liquid is first injected into the reaction cell module containing hemolytic agent among the at least 2 reaction cell modules.

[0122] In this example, before injecting the collected milliliter-level non-sample liquid into all the reaction cell modules, the reaction cell modules containing hemolytic agent in all the reaction cell modules can be determined first. Since the hemolytic agent can dissolve the protein in the blood sample, the protein is likely to adhere to the pipeline devices, and usually the amount of blood sample in the reaction cell module containing hemolytic agent is relatively large. Therefore, the collected milliliter-level non-sample liquid can be first injected into the reaction cell modules containing hemolytic agent in all the reaction cell modules, and then the collected milliliter-level non-sample liquid is injected into the reaction cell modules without hemolytic agent in all the reaction cell modules, so that the reaction cell modules containing hemolytic agent can have more soaking time of the milliliter-level non-sample liquid than the reaction cell modules without hemolytic agent, enhancing the cleaning effect on the reaction cell modules containing hemolytic agent.

[0123] Optionally, when the second depolymerization module 8 is connected to the first end of the first depolymerization module 6 through the third pipeline module 7, the driving power of the second depolymerization module 8 during the extraction operation or the pushing operation is the first power;

[0124] When the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, the driving power of the second depolymerization module 8 during the extraction operation or the pushing operation is the second power;

[0125] The first power is less than the second power.

[0126] Specifically, when the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, as opposed to when the second depolymerization module 8 is connected to the first end of the first depolymerization module 6 through the third pipeline module 7, the connection between the second depolymerization module 8 and the sample preparation module 1 is indirect, with the first depolymerization module 6 in between. Therefore, the resistance of the second depolymerization module 8 during the extraction operation or the pushing operation is relatively large. Based on the above method, by making the first power less than the second power, it can be ensured that in both cases, the ability of the second depolymerization module 8 to drive the liquid is strong enough to ensure the smooth progress of the extraction operation or the pushing operation, thereby improving the reliability of the blood polymerization parameter detector.

[0127] In one embodiment, as Figure 1 and Figure 2 shown, the blood polymerization parameter detector may further include a cleaning swab 11, which is used to be sleeved on the sample preparation module 1 to clean the outer wall of the sample preparation module 1.

[0128] Specifically, in practice, the cleaning swab 11 may have a liquid inlet and a liquid outlet. The liquid inlet may be connected to the second depolymerization module 8 through a corresponding two-way valve, and the liquid outlet may be connected to a waste liquid pool. For example, the diluent inhaled by the second depolymerization module 8 may be input through the liquid inlet so that the diluent can clean the outer wall of the sample preparation module 1, and then the waste liquid after cleaning may be output through the liquid outlet and discharged into the waste liquid pool or other types of waste liquid channels to complete the cleaning.

[0129] Based on the above method, since the capacity of the second depolymerization module 8 is relatively large, it can improve the cleaning effect of the cleaning swab 11 and also improve the cleaning efficiency.

[0130] In the description of the present application, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0131] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include at least one such feature. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise specifically defined.

[0132] Any process or method description represented in a flowchart or otherwise described herein may be understood to represent a module, segment, or portion of code including one or more executable instructions for implementing a specific logical function or process, and the scope of the preferred embodiments of the present application includes additional implementations, where the functions may be executed in a substantially simultaneous manner or in a reverse order according to the functions involved, rather than in the order shown or discussed, which should be understood by those skilled in the art to which the embodiments of the present application pertain.

[0133] The logic and / or steps represented in a flowchart or otherwise described herein, for example, may be considered as an ordered listing of executable instructions for implementing logical functions and may be specifically implemented in any computer-readable medium for use by or in connection with an instruction execution system, apparatus, or device (which may be a personal computer, a server, a network device, or other systems that can fetch and execute instructions from the instruction execution system, apparatus, or device). For the purposes of this specification, a "computer-readable medium" may be any device that can contain, store, communicate, propagate, or transport a program for use by or in connection with an instruction execution system, apparatus, or device. More specific examples (a non-exhaustive list) of the computer-readable medium include the following: an electrical connection portion with one or more wirings (electronic device), a portable computer diskette (magnetic device), a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber device, and a portable compact disc read-only memory (CDROM). Additionally, the computer-readable medium may even be paper or other suitable media on which the program can be printed, as the program can be obtained electronically, for example, by optically scanning the paper or other media, followed by editing, interpretation, or other appropriate processing as necessary, and then stored in a computer memory.

[0134] The above description is only for the embodiments of the present application and does not limit the patent scope of the present application. Any equivalent structural or equivalent process transformation made by using the content of the specification and drawings of the present application, or directly or indirectly applied in other related technical fields, shall be similarly included in the patent protection scope of the present application.

Claims

1. A blood polymerization parameter detector, characterized in that: include: A sample preparation module, the sample preparation module is used to prepare a blood sample to be tested; a first aggregation reduction module, wherein a first end of the first aggregation reduction module is connected to the sample preparation module; a first pipeline module, wherein the second end of the first reduction module is connected to the first end of the first pipeline module; a second aggregation reduction module, wherein the second end of the first pipeline module is connected to the first end of the second aggregation reduction module; a second pipeline module, wherein the second end of the second reduction module is connected to the first end of the second pipeline module; a first depolymerization module, wherein the second end of the second pipeline module is connected to the first end of the first depolymerization module; The third pipeline module; a second depolymerization module, wherein the second depolymerization module is connected to the first end or the second end of the first depolymerization module through the third pipeline module; A polymerization parameter detection module, the polymerization parameter detection module is arranged on the first pipeline module or the second pipeline module, and the polymerization parameter detection module is used to detect the polymerization parameter of the blood sample in the first pipeline module or the second pipeline module; Wherein, when the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the capacity of the first depolymerization module is greater than or equal to 5 and less than or equal to 50, Or, when the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the first depolymerization module is greater than or equal to 10 and less than or equal to 100.

2. The blood polymerization parameter detector according to claim 1, characterized in that: When the first deaggregation module and / or the second deaggregation module aspirates the blood sample, The maximum displacement of one end of the blood sample is greater than 1 / 2 of the length of the inner cavity of the sample preparation module, and / or, the blood sample passes through the first pipeline module, And / or, the blood sample does not pass through the second deagglomeration module.

3. The blood polymerization parameter detector according to claim 1, characterized in that: The orientation of the opening at one end of the first deagglomeration module is different from the orientation of the opening at the other end of the first deagglomeration module; and / or, the orientation of the opening at one end of the second aggregation reduction module is different from the orientation of the opening at the other end of the second aggregation reduction module; and / or, the blood sample undergoes at least one 180-degree turn when passing through the first deagglomeration module; and / or, the blood sample undergoes at least one 180-degree turn when passing through the second deagglomeration module; and / or, the minimum pipe diameter of the second deagglomeration module is different from the pipe diameter of the first pipeline module; and / or, the minimum pipe diameter of the second deagglomeration module is different from the pipe diameter of the second pipeline module; and / or, the maximum pipe diameter of the second deagglomeration module is different from the pipe diameter of the first pipeline module; and / or, the maximum pipe diameter of the second deagglomeration module is different from the pipe diameter of the second pipeline module; And / or, the length of the first pipeline module is greater than or equal to 5 cm.

4. The blood polymerization parameter detector according to claim 1, characterized in that: The extension direction of the inner cavity of the sample preparation module is parallel to the extension direction of the inner cavity of the first pipeline module, and the distance between the projection of the sample preparation module on the horizontal plane and the projection of the first pipeline module on the horizontal plane is less than 6 cm; And / or, a distance between a projection of one end of the first deagglomeration module on the horizontal plane and a projection of the other end of the first deagglomeration module on the horizontal plane is less than 6 cm.

5. The blood polymerization parameter detector according to claim 1, characterized in that: The first pipeline module includes a first sub-pipeline module and a second sub-pipeline module connected to each other; The two ends of the first sub-pipeline module are relatively fixed, so that the inner cavity axis of the first sub-pipeline module is kept as a straight line perpendicular to the horizontal plane; One end of the second sub-pipeline module is connected to one end of the first sub-pipeline module, and the other end of the second sub-pipeline module is connected to the second deagglomeration module. The second sub-pipeline module can perform corresponding deformation and / or movement when one end of the second sub-pipeline module and the other end of the second sub-pipeline module move relative to each other.

6. The blood polymerization parameter detector according to claim 5, characterized in that: When the aggregation parameter detection module detects the aggregation parameter, the distances among the aggregation parameter detection module, the first sub-pipeline module, and the sample preparation module remain unchanged; and / or; when the aggregation parameter detection module detects the aggregation parameter, keeping the first aggregation reduction module stationary and keeping the sample preparation module stationary in the vertical direction; and / or; when the polymerization parameter detection module detects the polymerization parameter, keeping the polymerization parameter detection module, the first pipeline module, and the sample preparation module stationary.

7. The blood polymerization parameter detector according to claim 6, characterized in that: The blood polymerization parameter detector also includes: a first connector module, the first connector module being respectively connected to the first deagglomeration module and the first sub-pipeline module; a second connector module, the second connector module being respectively connected to the first sub-pipeline module and the second sub-pipeline module; The pipe material parameters of any two adjacent modules among the first joint module, the first sub-pipeline module, the second joint module and the second sub-pipeline module are different. and / or, the first deagglomeration module and the first sub-pipeline module have different pipe material parameters, and / or, the first sub-pipeline module and the second sub-pipeline module have different pipe material parameters; The pipe material parameters include at least one of the pipe inner wall roughness, material, shape and pipe diameter.

8. The blood polymerization parameter detector according to claim 1, characterized in that: When the module with a larger capacity among the first deaggregation module and the second deaggregation module sucks and discharges the blood sample for deaggregation, the module with a smaller capacity among the first deaggregation module and the second deaggregation module also sucks and discharges the blood sample for deaggregation.

9. The blood polymerization parameter detector according to claim 8, characterized in that: When both the first deaggregation module and the second deaggregation module suck and discharge the blood sample for deaggregation, the deaggregation speed of the module with a smaller capacity among the first deaggregation module and the second deaggregation module is greater than the deaggregation speed of the module with a larger capacity among the first deaggregation module and the second deaggregation module; And / or, the driving speed of the first deaggregation module during blood separation is lower than the driving speed of the first deaggregation module during deaggregation.

10. The blood polymerization parameter detector according to claim 1, characterized in that: The first deaggregation module and / or the second deaggregation module are used for: Before the blood sample reaches the aggregation parameter detection module, the first deaggregation module and / or the second deaggregation module aspirates and exhales the blood sample for deaggregation.

11. The blood polymerization parameter detector according to claim 1, characterized in that: The capacity of the first depolymerization module is less than or equal to three times the maximum blood sample volume required for testing other than polymerization parameter testing in a single sample test. Or, the capacity of the first depolymerization module is greater than the maximum optical detection sample volume required for detection other than polymerization parameter detection in a single sample detection; And / or, a capacity ratio of the second deaggregation module to the first deaggregation module is greater than or equal to 40 and less than or equal to 120.

12. The blood polymerization parameter detector according to claim 1, characterized in that: The blood polymerization parameter detector also includes a reaction pool module; When the sample preparation module pushes the sample to the reaction pool module, the first depolymerization module must perform the pushing operation, and the second depolymerization module must not perform the pushing operation. and / or, when the sample preparation module pushes a microliter non-sample liquid to the reaction pool module, the first depolymerization module must perform a pushing operation, and / or, when the sample preparation module extracts microliter non-sample liquid, the first depolymerization module must perform an extraction operation, and / or, when the sample preparation module pushes milliliter-level non-sample liquid to the reaction pool module, the second depolymerization module must perform a pushing operation, And / or, when liquid is extracted by the sample preparation module, the second depolymerization module must perform an extraction operation.

13. The blood polymerization parameter detector according to claim 1, characterized in that: The blood polymerization parameter detector also includes at least two reaction pool modules. In the process of pushing the milliliter-level non-sample liquid to the reaction pool module through the first pipeline module and the sample preparation module in sequence, First, the collected milliliter-level non-sample liquid is injected into the reaction pool module containing the hemolytic agent among the at least two reaction pool modules.

14. The blood polymerization parameter detector according to claim 1, characterized in that: When the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the driving power of the second depolymerization module during the extraction operation or the pushing operation is the first power; When the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the driving power of the second depolymerization module during the extraction operation or the pushing operation is the second power; The first power is less than the second power.

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