Device and method for rapidly evaluating antifouling property of material by simulating marine environment

Abstract

The invention discloses a device and a method for quickly evaluating the antifouling property of a material for simulating a marine environment. The device comprises a glass jar, an ecological simulation unit, a water circulation filtering unit, a temperature adjusting unit, a water body protein decomposer, an illumination simulation unit, an oxygen making device, a quartz crystal microbalance, a material sample suspension assembly, a chlorophyll probe, a multi-dimensional analysis system, a fixed supporting table, a movable supporting table, a wave making pump and a temperature sensor. According to the rapid evaluation method for the antifouling performance of the material for simulating the marine environment, integrated coupling of a multi-dimensional environment control technology, an ecological simulation technology and a real-time data acquisition technology is achieved, more comprehensive and accurate biological fouling evaluation can be achieved, accurate control over key environmental factors can be achieved, and the evaluation efficiency is improved. The marine environment and a complex ecological system can be simulated, meanwhile, the growth and attachment conditions of fouling organisms on the surface of the material can be monitored in real time, and rapid and accurate technical support is provided for development and performance optimization of the antifouling material.

Description

Technical Field

[0001] The invention belongs to the field of marine environment material science and technology, and in particular relates to a device and method for quickly evaluating the antifouling performance of materials simulating a marine environment. Background Art

[0002] Marine biofouling refers to the phenomenon of algae, shellfish, bacteria and other marine organisms attaching to the surface of ships, marine engineering equipment and other marine facilities. This process can lead to equipment corrosion, increased energy consumption, performance degradation, and significantly increase maintenance costs.

[0003] Currently, the evaluation of antifouling materials mostly relies on tests in natural environments. This method not only has a long cycle, but is also easily affected by external environmental factors, resulting in large fluctuations in test results and poor repeatability, making it difficult to provide timely and effective support for the research and development of new materials.

[0004] The Chinese patent application with application number 202311685856.2 discloses a semi-open propulsion structure biological fouling test device under simulated working conditions. This scheme focuses on the simulation of physical structures and fails to fully control the key environmental factors that affect the growth of fouling organisms, such as oxygen concentration, light intensity, water quality changes, etc. It lacks the ability of real-time monitoring, which affects the accuracy and timeliness of the evaluation results.

[0005] The Chinese patent application with application number 202311743742.9 discloses an automated simulation test device for biological fouling. Although this solution can simulate static and dynamic water flow environments, it has the problem of complex equipment structure design, which increases the difficulty of equipment operation and maintenance. At the same time, it does not provide sufficient environmental adjustment means and cannot effectively ensure the normal growth of fouling organisms.

[0006] The Chinese patent application with application number 202020179211.7 discloses a continuous accelerated evaluation device for microbial corrosion and biofouling. This scheme adopts electrochemical measurement method and relies too much on single electrochemical data. It fails to fully reflect the physical attachment characteristics of biofouling, resulting in insufficient representativeness of the test results.

[0007] Chinese patent application with application number 201710253848.9 discloses a method for monitoring and evaluating early marine biofouling. This scheme is only applicable to transparent materials and requires spectrophotometric measurement through periodic sampling. It cannot achieve real-time monitoring and its scope of application is limited.

[0008] The Chinese patent application with application number 201611030006.9 discloses a method for testing the anti-biological fouling performance of anti-fouling materials. This scheme evaluates the anti-fouling performance by measuring physical parameters such as contact angle and rolling angle, but ignores the effect of chemical properties on biological attachment, especially for anti-fouling materials that rely on chemical inhibitory components, and cannot accurately reflect their actual effects.

[0009] In summary, although the existing schemes have played a certain role in accelerating the evaluation process, the singleness and limitations of their technical means still affect the accuracy and efficiency of the test. The existing schemes usually only control a few environmental factors such as temperature or light, and lack comprehensive simulation of multiple key factors such as oxygen concentration and water quality. In addition, most of the existing schemes rely on manual operations for data collection, which cannot achieve real-time monitoring and is difficult to simulate actual complex ecosystems. Therefore, these shortcomings of the existing schemes have led to a large deviation between the experimental results and actual applications, thus affecting the comprehensive evaluation of antifouling materials. Summary of the invention

[0010] In view of the problems existing in the prior art, the present invention provides a device and method for quickly evaluating the antifouling performance of materials in a simulated marine environment, which realizes the integrated coupling of multi-dimensional environmental control technology, ecological simulation technology, and real-time data acquisition technology, can achieve a more comprehensive and accurate biological fouling assessment, can achieve precise control of key environmental factors, can simulate the marine environment and complex ecosystems, and can realize real-time monitoring of the growth and attachment of fouling organisms on the surface of materials, thereby providing rapid and accurate technical support for the development and performance optimization of antifouling materials.

[0011] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a device for quickly evaluating the antifouling performance of materials simulating a marine environment, comprising a glass cylinder, an ecological simulation unit, a water circulation filtration unit, a temperature regulating unit, a water protein decomposer, a light simulation unit, an oxygenator, a quartz crystal microbalance, a material sample suspension assembly, a chlorophyll probe, a multi-dimensional analysis system, a fixed support platform and a mobile support platform, a wave-making pump and a temperature sensor; the glass cylinder is installed above the fixed support platform; the ecological simulation unit is located inside the glass cylinder; the water circulation filtration unit is arranged inside the fixed support platform below the glass cylinder; the temperature regulating unit is arranged between the water circulation filtration unit and the glass cylinder; the water protein decomposer is arranged outside the glass cylinder, and the water inlet pipe and the water outlet pipe of the water protein decomposer extend to the inside of the glass cylinder; the light simulation unit is located above the glass cylinder; the oxygenator is arranged inside the glass cylinder; the quartz crystal microbalance is arranged above the mobile support platform, and the mobile support platform and the fixed support platform are distributed in parallel; the material sample suspension assembly is arranged between the quartz crystal microbalance and the glass cylinder; the chlorophyll probe is arranged inside the glass cylinder; the wave-making pump is arranged inside the glass cylinder; the temperature sensor is arranged inside the glass cylinder; and the multi-dimensional analysis system is arranged outside the glass cylinder.

[0012] The ecological simulation unit includes artificial seawater, fouled biological stones and clownfish; the artificial seawater is filled in a glass tank; the fouled biological stones are immersed in the artificial seawater and placed at the bottom of the glass tank; the clownfish are cultured in the artificial seawater; the chlorophyll probe is installed on the fouled biological stones; the water inlet pipe and the water outlet pipe of the water body protein decomposer are both extended to the artificial seawater; the wave-making pump is immersed in the artificial seawater and installed on the inner wall of the glass tank; the temperature sensor is immersed in the artificial seawater and installed on the inner wall of the glass tank.

[0013] The water circulation filtration unit includes a coarse filter barrel and a fine filter barrel; the coarse filter barrel and the fine filter barrel are placed side by side in a fixed support platform; the lower water inlet of the coarse filter barrel is connected to the artificial seawater inside the glass tank through a pipeline, the upper water outlet of the coarse filter barrel is connected to the lower water inlet of the fine filter barrel through a pipeline, and the upper water outlet of the fine filter barrel is connected to the temperature adjustment unit through a pipeline.

[0014] The temperature regulating unit includes a chiller and a heater; the water inlet of the chiller is connected to the upper water outlet of the fine filter barrel through a pipeline, the water outlet of the chiller is connected to the water inlet of the pipeline heater through a pipeline, and the water outlet of the heater extends to above the surface of the artificial seawater inside the glass cylinder through a pipeline.

[0015] The illumination simulation unit comprises an LED lighting lamp and a lamp stand; the number of the LED lighting lamp is at least one; the lamp stand is fixedly mounted on the glass tank, and the LED lighting lamp is suspended on the lamp stand and is located above the artificial seawater.

[0016] The material sample suspension assembly includes a cantilever force transmission rod, a hanging rope and a hanging rope; the hanging rope is horizontally arranged on the top of the glass cylinder; one end of the cantilever force transmission rod is connected to the measuring end of the quartz crystal microbalance, and the other end of the cantilever force transmission rod is connected to the hanging rope; the upper end of the hanging rope is connected to the hanging rope, and the material sample is installed at the lower end of the hanging rope, and the material sample is immersed in artificial seawater.

[0017] The multidimensional analysis system includes a SLR camera, a fluorescence microscope, a laser confocal microscope, an atomic force microscope, a scanning electron microscope and a cryo-electron microscope, and the SLR camera, the fluorescence microscope, the laser confocal microscope, the atomic force microscope, the scanning electron microscope and the cryo-electron microscope are all independently set up under laboratory conditions.

[0018] A method for quickly evaluating the antifouling performance of materials in a simulated marine environment, using the device for quickly evaluating the antifouling performance of materials in a simulated marine environment, specifically:

[0019] 1. Construction of simulated marine environment

[0020] ① Start the chiller. Under the pumping action of the chiller, the artificial seawater circulates between the glass tank, the coarse filter barrel, the fine filter barrel, the chiller and the heater. Impurities in the artificial seawater are removed through the coarse filter barrel and the fine filter barrel. The temperature of the artificial seawater is monitored in real time through the temperature sensor. The heater is dynamically started to adjust the temperature of the artificial seawater until the temperature of the artificial seawater reaches the set value.

[0021] ②. Start the water protein decomposer, oxygenator and LED lighting. Remove organic matter from artificial seawater through the water protein decomposer to maintain the stability of artificial seawater quality. Provide intermittent oxygen supply through the oxygenator to simulate the fluctuation of oxygen in the marine environment. Use LED lighting to simulate the changes in lighting conditions and intensity under the alternation of day and night.

[0022] ③. After the simulated ocean environment is constructed, the artificial seawater will continue to run stably without load for 2-3 days until all water indicators of the artificial seawater meet the set requirements. Then, the fouled biological rocks and clownfish collected from the natural sea area will be sent into the artificial seawater, and then the wave pump will be dynamically started to simulate the water flow conditions in the ocean environment.

[0023] 2. Evaluation of antifouling performance of material samples

[0024] ①. Use a quartz crystal microbalance to measure the mass of fouling organisms growing on the surface of the material sample, and evaluate the antifouling performance of the material sample by the mass change of the fouling organisms on the surface of the material sample;

[0025] ② Monitor the growth of fouling organisms in artificial seawater through chlorophyll probes and evaluate changes in simulated marine environmental conditions;

[0026] ③. Take out material samples regularly, take photos of the surface of the material samples with a SLR camera to obtain images, analyze the stain coverage rate at different time points through the surface images of the material samples, and evaluate the anti-fouling performance of the material samples at the macro level through the stain coverage rate;

[0027] ④. Take out material samples regularly, and use a fluorescence microscope to stain and observe the fouling organisms attached to the surface of the material samples. Use fluorescent dyes to mark the proteins and polysaccharide components in the cells of the fouling organisms, observe the attachment of the fouling organisms, and evaluate the antifouling performance of the material samples at the microscopic level by the staining of the proteins and polysaccharide components in the cells of the fouling organisms. At the same time, use a fluorescence microscope to regularly photograph different areas on the surface of the material samples, compare the fluorescence images at different time points, analyze the attachment process of the fouling organisms and the distribution of the fouling organisms on the surface of the material samples, and further evaluate the antifouling performance of the material samples.

[0028] ⑤. Take out material samples regularly, obtain high-resolution three-dimensional images of fouling organisms attached to the surface of material samples through laser confocal microscopy, evaluate the impact of material samples on the morphology and growth behavior of fouling organisms, and evaluate the antifouling performance of material samples at the microscopic level through the morphology and growth behavior of fouling organisms; at the same time, obtain the attachment thickness, density and structural characteristics of fouling organisms through layer-by-layer scanning and three-dimensional modeling, so as to determine the formation process and stability of fouling organisms, and further evaluate the antifouling performance of material samples;

[0029] ⑥ Regularly take out material samples and use atomic force microscopy to test the adhesion of fouling organisms attached to the surface of the material samples. The probe of the atomic force microscope makes physical contact with the fouling organisms to measure the adhesion strength and surface roughness of the fouling organisms. At the microscopic level, the anti-fouling performance of the material samples is evaluated by the adhesion of the fouling organisms.

[0030] ⑦. Take out material samples regularly, and use scanning electron microscopy to perform microscopic imaging of fouling organisms attached to the surface of the material samples. Use the generated high-resolution microscopic images to observe the cell morphology, extracellular polymer distribution and microscopic tissue structure of the fouling organisms, and display the morphological characteristics of the fouling organisms. At the microscopic level, evaluate the antifouling performance of the material samples through the morphological characteristics of the fouling organisms;

[0031] ⑧. Take out material samples regularly and use cryo-electron microscopy to perform ultrastructural imaging of the fouling organisms attached to the surface of the material samples. The internal structural characteristics of the fouling biological cells are observed through the generated high-resolution ultramicroscopic images. At the microscopic level, the attachment mechanism of the fouling organisms and the anti-fouling mechanism of the material samples are analyzed, and the anti-fouling performance of the material samples is evaluated.

[0032] Beneficial effects of the present invention:

[0033] The device and method for rapid evaluation of the antifouling performance of materials in a simulated marine environment of the present invention can accelerate the marine biofouling process through multi-dimensional environmental control and real-time systematic monitoring under laboratory conditions, and quickly and accurately evaluate the antifouling performance of different types of antifouling materials. Combined with the comprehensive simulation of multiple key factors in the natural environment, the evaluation cycle of the antifouling performance of materials can be significantly shortened, and the accuracy and repeatability of the experimental results can be improved. It is particularly suitable for the rapid screening and optimization of surface protective materials for ships, marine facilities, etc. in the marine environment.

[0034] The device and method for rapid evaluation of antifouling performance of materials in a simulated marine environment of the present invention can simulate key factors such as temperature, light, oxygen concentration, water quality, etc. in the marine environment through multi-dimensional environmental control to accelerate the biological fouling process; through light regulation, it can accurately simulate the alternating day and night light and intensity changes in the natural marine environment to improve the representativeness of the test conditions; through intermittent oxygen supply, it can simulate the fluctuation of oxygen concentration in the ocean, which helps to maintain the dynamic balance of the experimental environment and promote the natural growth of fouling organisms; by introducing clownfish to simulate a complex real ecosystem, the authenticity of the experiment is further improved.

[0035] The device and method for quickly evaluating the antifouling performance of materials in a simulated marine environment of the present invention ensure the cleanliness and stability of the experimental water through water quality filtration and protein separation, and avoid the interference of organic matter and impurities on the experimental results; use a single-lens reflex camera to shoot the fouling coverage rate, use a fluorescence microscope and a laser confocal microscope to analyze the early attached proteins and polysaccharides, and use an atomic force microscope and a scanning electron microscope to evaluate the adhesion and microstructure of the biofilm, so as to achieve the purpose of comprehensively analyzing the antifouling performance of the material from macro to micro, and can provide rich experimental data support.

[0036] The device and method for quickly evaluating the antifouling performance of materials in a simulated marine environment of the present invention adopt a dynamic testing process based on time nodes. Material samples are suspended in an experimental environment and sampled and tested regularly, so as to dynamically observe changes in the antifouling effect. A quartz crystal microbalance suspension system is used to monitor the formation and quality changes of biofilms on the sample surfaces in real time. The device and method can detect the formation process of trace attachments on the material surface with high sensitivity, accurately analyze the growth rate and attachment amount of the biofilm, and realize dynamic monitoring of the antifouling performance.

[0037] The device and method for quickly evaluating the antifouling performance of materials in a simulated marine environment of the present invention simulate the dynamic water flow conditions in an actual marine environment by adjusting the water flow speed and direction, and are used to study the influence of water flow on biofilm formation and antifouling material performance by adjusting the water flow intensity and direction, which helps to further optimize the performance of antifouling materials under dynamic water flow conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a structural schematic diagram (overall) of a device for rapid evaluation of antifouling performance of materials simulating marine environment of the present invention;

[0039] Figure 2 This is a schematic diagram (partial) of the structure of a device for quickly evaluating the antifouling performance of materials in a simulated marine environment according to the present invention;

[0040] In the figure, 1 is a glass tank, 2 is a water protein decomposer, 3 is an oxygenator, 4 is a quartz crystal microbalance, 5 is a chlorophyll probe, 6 is a fixed support table, 7 is a mobile support table, 8 is a fouled biological stone, 9 is a clownfish, 10 is a coarse filter barrel, 11 is a fine filter barrel, 12 is a chiller, 13 is a heater, 14 is an LED lighting, 15 is a light stand, 16 is a cantilever force transmission rod, 17 is a hanging rope, 18 is a hanging rope, 19 is a material sample, 20 is a wave pump, and 21 is a temperature sensor. DETAILED DESCRIPTION

[0041] The present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0042] like Figure 1 , 2As shown, a rapid evaluation device for antifouling performance of materials simulating marine environment includes a glass cylinder 1, an ecological simulation unit, a water circulation filtration unit, a temperature regulating unit, a water protein decomposer 2, a light simulation unit, an oxygenator 3, a quartz crystal microbalance 4, a material sample suspension component, a chlorophyll probe 5, a multi-dimensional analysis system, a fixed support platform 6 and a mobile support platform 7, a wave pump 20 and a temperature sensor 21; the glass cylinder 1 is installed above the fixed support platform 6; the ecological simulation unit is located inside the glass cylinder 1; the water circulation filtration unit is arranged inside the fixed support platform 6 below the glass cylinder 1; the temperature regulating unit is arranged between the water circulation filtration unit and the glass cylinder 1 The water protein decomposer 2 is arranged outside the glass cylinder 1, and the water inlet pipe and the water outlet pipe of the water protein decomposer 2 extend to the inside of the glass cylinder 1; the light simulation unit is located above the glass cylinder 1; the oxygenator 3 is arranged inside the glass cylinder 1; the quartz crystal microbalance 4 is arranged above the mobile support platform 7, and the mobile support platform 7 and the fixed support platform 6 are arranged in parallel; the material sample suspension assembly is arranged between the quartz crystal microbalance 4 and the glass cylinder 1; the chlorophyll probe 5 is arranged inside the glass cylinder 1; the wave pump 20 is arranged inside the glass cylinder 1; the temperature sensor 21 is arranged inside the glass cylinder 1; the multi-dimensional analysis system is arranged outside the glass cylinder 1.

[0043] The ecological simulation unit includes artificial seawater, fouling biological stone 8 and clownfish 9; the artificial seawater is filled in the glass tank 1; the fouling biological stone 8 is immersed in the artificial seawater and placed at the bottom of the glass tank 1; the clownfish 9 is cultured in the artificial seawater; the chlorophyll probe 5 is installed on the fouling biological stone 8; the water inlet pipe and the water outlet pipe of the water body protein decomposer 2 are extended to the artificial seawater; the wave pump 20 is immersed in the artificial seawater and installed on the inner wall of the glass tank 1; the temperature sensor 21 is immersed in the artificial seawater and installed on the inner wall of the glass tank 1. Specifically, the temperature sensor 21 uses a visual thermometer and is directly attached to the inner wall of the glass tank 1; the water inlet of the wave pump 20 faces downward, and the level of the wave pump 20 faces the fouling biological stone 8.

[0044] The water circulation filtration unit includes a coarse filter barrel 10 and a fine filter barrel 11; the coarse filter barrel 10 and the fine filter barrel 11 are placed side by side in a fixed support platform 6; the lower water inlet of the coarse filter barrel 10 is connected to the artificial seawater inside the glass tank 1 through a pipeline, the upper water outlet of the coarse filter barrel 10 is connected to the lower water inlet of the fine filter barrel 11 through a pipeline, and the upper water outlet of the fine filter barrel 11 is connected to the temperature adjustment unit through a pipeline. Specifically, the coarse filter barrel 10 is filled with only filter cotton, and the fine filter barrel 11 is filled with filter materials such as quartz stone and sponge.

[0045] The temperature adjustment unit includes a chiller 12 and a heater 13; the water inlet of the chiller 12 is connected to the upper water outlet of the fine filter barrel 11 through a pipeline, the water outlet of the chiller 12 is connected to the water inlet of the pipeline heater 13 through a pipeline, and the water outlet of the heater 13 extends to the surface of the artificial seawater inside the glass tank 1 through a pipeline. Specifically, the distance between the outlet pipe of the heater 13 and the surface of the artificial seawater is set to 1 cm.

[0046] The illumination simulation unit includes an LED lighting lamp 14 and a lamp holder 15; the number of the LED lighting lamp 14 is at least one; the lamp holder 15 is fixedly mounted on the glass tank 1, and the LED lighting lamp 14 is suspended on the lamp holder 15 and is located above the artificial seawater. Specifically, the lamp holder 15 adopts an L-shaped structure, the vertical arm of the lamp holder 15 is fixedly connected to the glass tank 1, and the LED lighting lamp 14 is suspended at the end of the horizontal arm of the lamp holder 15; the number of the LED lighting lamps 14 is two, and the two LED lighting lamps 14 have the same height and are arranged in parallel.

[0047] The material sample suspension assembly includes a cantilever force transmission rod 16, a hanging rope 17 and a hanging rope 18; the hanging rope 17 is horizontally arranged on the top of the glass cylinder 1; one end of the cantilever force transmission rod 16 is connected to the measuring end of the quartz crystal microbalance 4, and the other end of the cantilever force transmission rod 16 is connected to the hanging rope 17; the upper end of the hanging rope 18 is connected to the hanging rope 17, and the material sample 19 is installed at the lower end of the hanging rope 18, and the material sample 19 is immersed in artificial seawater.

[0048] The multidimensional analysis system includes a SLR camera, a fluorescence microscope, a laser confocal microscope, an atomic force microscope, a scanning electron microscope and a cryo-electron microscope, and the SLR camera, the fluorescence microscope, the laser confocal microscope, the atomic force microscope, the scanning electron microscope and the cryo-electron microscope are all independently set up under laboratory conditions.

[0049] A method for quickly evaluating the antifouling performance of materials in a simulated marine environment, using the device for quickly evaluating the antifouling performance of materials in a simulated marine environment, specifically:

[0050] 1. Construction of simulated marine environment

[0051] ①, start the chiller 12. Under the pumping action of the chiller 12, the artificial seawater circulates among the glass cylinder 1, the coarse filter barrel 10, the fine filter barrel 11, the chiller 12 and the heater 13. Impurities in the artificial seawater are removed by the coarse filter barrel 10 and the fine filter barrel 11. The temperature of the artificial seawater is monitored in real time by the temperature sensor 21. The heater 13 is dynamically started to adjust the temperature of the artificial seawater until the temperature of the artificial seawater reaches the set value; specifically, the temperature setting value of the artificial seawater is 25°C;

[0052] ②, start the water protein decomposer 2, the oxygenator 3 and the LED lighting 14, remove organic matter in the artificial seawater through the water protein decomposer 2 to maintain the stability of the artificial seawater quality, intermittently supply oxygen through the oxygenator 3 to simulate the fluctuation of oxygen in the marine environment, and simulate the changes in lighting conditions and intensity under the alternation of day and night through the LED lighting 14; specifically, the oxygenator 3 provides oxygen to the artificial seawater once every 30 minutes;

[0053] ③. After the simulated ocean environment is constructed, the artificial seawater continues to run stably without load for 2-3 days until all water indicators of the artificial seawater meet the set requirements. Then, the fouling biological rocks 8 and clownfish 9 collected from the natural sea area are sent into the artificial seawater, and then the wave pump 20 is dynamically started to simulate the water flow conditions in the ocean environment.

[0054] 2. Evaluation of antifouling performance of material samples

[0055] ①, measuring the mass of fouling organisms growing on the surface of the material sample 19 by using a quartz crystal microbalance 4, and evaluating the antifouling performance of the material sample 19 by the mass change of the fouling organisms on the surface of the material sample 19;

[0056] ②, monitor the growth of fouling organisms in artificial seawater through chlorophyll probe 5, and evaluate the changes in simulated marine environmental conditions;

[0057] ③. Periodically take out the material sample 19, take photos of the surface of the material sample 19 with a SLR camera to obtain images, analyze the contamination coverage at different time points through the surface images of the material sample 19, and evaluate the anti-fouling performance of the material sample 19 at a macro level through the contamination coverage;

[0058] ④. Periodically take out material sample 19, stain and observe the fouling organisms attached to the surface of material sample 19 through a fluorescence microscope, mark the protein and polysaccharide components in the cells of the fouling organisms with fluorescent dyes, observe the attachment of the fouling organisms, and evaluate the anti-fouling performance of material sample 19 at a microscopic level through the staining of the protein and polysaccharide components in the cells of the fouling organisms; at the same time, regularly photograph different areas on the surface of material sample 19 through a fluorescence microscope, compare the fluorescence images at different time points, analyze the attachment process of the fouling organisms and the distribution of the fouling organisms on the surface of material sample 19, and further evaluate the anti-fouling performance of material sample 19; specifically, fluorescence microscopy imaging technology is very suitable for detecting the initial biofilm formed by the fouling organisms on the sample surface, and is used to provide quantitative attachment data.

[0059] ⑤. Periodically take out material sample 19, obtain high-resolution three-dimensional images of fouling organisms attached to the surface of material sample 19 through a laser confocal microscope, evaluate the effect of material sample 19 on the morphology and growth behavior of fouling organisms, and evaluate the antifouling performance of material sample 19 at the microscopic level through the morphology and growth behavior of fouling organisms; at the same time, synchronously obtain the attachment thickness, density and structural characteristics of fouling organisms through layer-by-layer scanning three-dimensional modeling, determine the formation process and stability of fouling organisms, and further evaluate the antifouling performance of material sample 19;

[0060] ⑥. Periodically take out material sample 19, and conduct adhesion test on fouling organisms attached to the surface of material sample 19 through atomic force microscopy. The probe of the atomic force microscope makes physical contact with the fouling organisms to measure the adhesion strength and surface roughness of the fouling organisms. At the microscopic level, the antifouling performance of material sample 19 is evaluated through the adhesion of the fouling organisms. Specifically, by constructing the adhesion curve of the fouling organisms, the changes in the adhesion strength of the biofilm under different environmental conditions can be intuitively understood, thereby better realizing the evaluation of the antifouling performance of material sample 19.

[0061] ⑦. Periodically take out the material sample 19, and use a scanning electron microscope to perform microscopic imaging of the fouling organisms attached to the surface of the material sample 19. The cell morphology, extracellular polymer distribution and microscopic tissue structure of the fouling organisms are observed through the generated high-resolution microscopic images to display the morphological characteristics of the fouling organisms. At the microscopic level, the antifouling performance of the material sample 19 is evaluated through the morphological characteristics of the fouling organisms;

[0062] ⑧. Periodically take out material sample 19, and perform ultrastructural imaging of the fouling organisms attached to the surface of material sample 19 through cryo-electron microscopy. The internal structural characteristics of the fouling biological cells are observed through the generated high-resolution ultramicroscopic images. At the microscopic level, the attachment mechanism of the fouling organisms and the anti-fouling mechanism of material sample 19 are analyzed, and the anti-fouling performance of material sample 19 is evaluated.

[0063] The solutions in the embodiments are not intended to limit the protection scope of the present invention. All equivalent implementations or changes that do not deviate from the present invention are included in the protection scope of the present invention.

Claims

1. A rapid evaluation device for antifouling performance of materials simulating marine environment, characterized by: It includes a glass cylinder, an ecological simulation unit, a water circulation filtration unit, a temperature regulating unit, a water protein decomposer, a light simulation unit, an oxygenator, a quartz crystal microbalance, a material sample suspension component, a chlorophyll probe, a multi-dimensional analysis system, a fixed support platform and a mobile support platform, a wave pump and a temperature sensor; the glass cylinder is installed above the fixed support platform; the ecological simulation unit is located inside the glass cylinder; the water circulation filtration unit is arranged inside the fixed support platform below the glass cylinder; the temperature regulating unit is arranged between the water circulation filtration unit and the glass cylinder; the water protein decomposer is arranged outside the glass cylinder, and the water inlet pipe and the water outlet pipe of the water protein decomposer extend to the inside of the glass cylinder; The light simulation unit is located above the glass cylinder; the oxygen generator is arranged inside the glass cylinder; the quartz crystal microbalance is arranged above the movable support platform, and the movable support platform and the fixed support platform are distributed in parallel; the material sample suspension assembly is arranged between the quartz crystal microbalance and the glass cylinder; the chlorophyll probe is arranged inside the glass cylinder; the wave-making pump is arranged inside the glass cylinder; the temperature sensor is arranged inside the glass cylinder; and the multi-dimensional analysis system is arranged outside the glass cylinder.

2. The device for rapid evaluation of antifouling performance of materials in a simulated marine environment according to claim 1, characterized in that: The ecological simulation unit includes artificial seawater, fouled biological stones and clownfish; the artificial seawater is filled in a glass tank; the fouled biological stones are immersed in the artificial seawater and placed at the bottom of the glass tank; the clownfish are cultured in the artificial seawater; the chlorophyll probe is installed on the fouled biological stones; the water inlet pipe and the water outlet pipe of the water body protein decomposer are both extended to the artificial seawater; the wave-making pump is immersed in the artificial seawater and installed on the inner wall of the glass tank; the temperature sensor is immersed in the artificial seawater and installed on the inner wall of the glass tank.

3. The device for rapid evaluation of antifouling performance of materials in a simulated marine environment according to claim 2, characterized in that: The water circulation filtration unit includes a coarse filter barrel and a fine filter barrel; the coarse filter barrel and the fine filter barrel are placed side by side in a fixed support platform; the lower water inlet of the coarse filter barrel is connected to the artificial seawater inside the glass tank through a pipeline, the upper water outlet of the coarse filter barrel is connected to the lower water inlet of the fine filter barrel through a pipeline, and the upper water outlet of the fine filter barrel is connected to the temperature adjustment unit through a pipeline.

4. The device for rapid evaluation of antifouling performance of materials in a simulated marine environment according to claim 3, characterized in that: The temperature regulating unit includes a chiller and a heater; the water inlet of the chiller is connected to the upper water outlet of the fine filter barrel through a pipeline, the water outlet of the chiller is connected to the water inlet of the pipeline heater through a pipeline, and the water outlet of the heater extends to above the surface of the artificial seawater inside the glass cylinder through a pipeline.

5. The device for rapid evaluation of antifouling performance of materials in a simulated marine environment according to claim 4, characterized in that: The illumination simulation unit comprises an LED lighting lamp and a lamp stand; the number of the LED lighting lamp is at least one; the lamp stand is fixedly mounted on the glass tank, and the LED lighting lamp is suspended on the lamp stand and is located above the artificial seawater.

6. The device for rapid evaluation of antifouling performance of materials in a simulated marine environment according to claim 5, characterized in that: The material sample suspension assembly includes a cantilever force transmission rod, a hanging rope and a hanging rope; the hanging rope is horizontally arranged on the top of the glass cylinder; one end of the cantilever force transmission rod is connected to the measuring end of the quartz crystal microbalance, and the other end of the cantilever force transmission rod is connected to the hanging rope; the upper end of the hanging rope is connected to the hanging rope, and the material sample is installed at the lower end of the hanging rope, and the material sample is immersed in artificial seawater.

7. The device for rapid evaluation of antifouling performance of materials in a simulated marine environment according to claim 6, characterized in that: The multidimensional analysis system includes a SLR camera, a fluorescence microscope, a laser confocal microscope, an atomic force microscope, a scanning electron microscope and a cryo-electron microscope, and the SLR camera, the fluorescence microscope, the laser confocal microscope, the atomic force microscope, the scanning electron microscope and the cryo-electron microscope are all independently set up under laboratory conditions.

8. A method for quickly evaluating the antifouling performance of materials in a simulated marine environment, using the device for quickly evaluating the antifouling performance of materials in a simulated marine environment as claimed in claim 7, characterized in that: Specifically:

1. Construction of simulated marine environment ① Start the chiller. Under the pumping action of the chiller, the artificial seawater circulates between the glass tank, the coarse filter barrel, the fine filter barrel, the chiller and the heater. Impurities in the artificial seawater are removed through the coarse filter barrel and the fine filter barrel. The temperature of the artificial seawater is monitored in real time through the temperature sensor. The heater is dynamically started to adjust the temperature of the artificial seawater until the temperature of the artificial seawater reaches the set value. ②. Start the water protein decomposer, oxygenator and LED lighting. Remove organic matter from artificial seawater through the water protein decomposer to maintain the stability of artificial seawater quality. Provide intermittent oxygen supply through the oxygenator to simulate the fluctuation of oxygen in the marine environment. Use LED lighting to simulate the changes in lighting conditions and intensity under the alternation of day and night. ③. After the simulated ocean environment is constructed, the artificial seawater will continue to run stably without load for 2-3 days until all water indicators of the artificial seawater meet the set requirements. Then, the fouled biological rocks and clownfish collected from the natural sea area will be sent into the artificial seawater, and then the wave pump will be dynamically started to simulate the water flow conditions in the ocean environment.

2. Evaluation of antifouling performance of material samples ①. Use a quartz crystal microbalance to measure the mass of fouling organisms growing on the surface of the material sample, and evaluate the antifouling performance of the material sample by the mass change of the fouling organisms on the surface of the material sample; ② Monitor the growth of fouling organisms in artificial seawater through chlorophyll probes and evaluate changes in simulated marine environmental conditions; ③. Take out material samples regularly, take photos of the surface of the material samples with a SLR camera to obtain images, analyze the stain coverage rate at different time points through the surface images of the material samples, and evaluate the anti-fouling performance of the material samples at the macro level through the stain coverage rate; ④. Take out material samples regularly, and use a fluorescence microscope to stain and observe the fouling organisms attached to the surface of the material samples. Use fluorescent dyes to mark the proteins and polysaccharide components in the cells of the fouling organisms, observe the attachment of the fouling organisms, and evaluate the antifouling performance of the material samples at the microscopic level by the staining of the proteins and polysaccharide components in the cells of the fouling organisms. At the same time, use a fluorescence microscope to regularly photograph different areas on the surface of the material samples, compare the fluorescence images at different time points, analyze the attachment process of the fouling organisms and the distribution of the fouling organisms on the surface of the material samples, and further evaluate the antifouling performance of the material samples. ⑤. Take out material samples regularly, obtain high-resolution three-dimensional images of fouling organisms attached to the surface of the material samples through laser confocal microscopy, evaluate the impact of material samples on the morphology and growth behavior of fouling organisms, and evaluate the anti-fouling performance of material samples at the microscopic level through the morphology and growth behavior of fouling organisms; at the same time, obtain the attachment thickness, density and structural characteristics of fouling organisms through layer-by-layer scanning and three-dimensional modeling, so as to determine the formation process and stability of fouling organisms, and further evaluate the anti-fouling performance of material samples; ⑥ Regularly take out material samples and use atomic force microscopy to test the adhesion of fouling organisms attached to the surface of the material samples. The probe of the atomic force microscope makes physical contact with the fouling organisms to measure the adhesion strength and surface roughness of the fouling organisms. At the microscopic level, the anti-fouling performance of the material samples is evaluated by the adhesion of the fouling organisms. ⑦. Take out material samples regularly, and use scanning electron microscopy to perform microscopic imaging of fouling organisms attached to the surface of the material samples. Use the generated high-resolution microscopic images to observe the cell morphology, extracellular polymer distribution and microscopic tissue structure of the fouling organisms, and display the morphological characteristics of the fouling organisms. At the microscopic level, evaluate the antifouling performance of the material samples through the morphological characteristics of the fouling organisms; ⑧. Take out material samples regularly and use cryo-electron microscopy to perform ultrastructural imaging of the fouling organisms attached to the surface of the material samples. The internal structural characteristics of the fouling biological cells are observed through the generated high-resolution ultramicroscopic images. At the microscopic level, the attachment mechanism of the fouling organisms and the anti-fouling mechanism of the material samples are analyzed, and the anti-fouling performance of the material samples is evaluated.

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