A p-hydroxyphenylglycine fermentation enzyme sample detection device

Abstract

The invention provides a para-hydroxyphenylglycine fermentation enzyme sample detection device, which relates to the technical field of fermentation sample detection; it comprises a detection mounting part, on which a detection conveying part is installed; on which a lifting conveying part is installed; the lifting conveying part is used to take a crystal sample in a fermentation tank; a suction single-layer sampling part is installed on the side of the detection conveying part; the suction single-layer sampling part is used for suction sampling, and the suction single-layer sampling part can facilitate the sampling of single crystals, which can make staining more convenient and prevent messy crystals from affecting microscopic observation, and can also play a role in absorbing liquid, reducing the influence of fermentation liquid on staining, thereby solving the problem that the traditional manual sampling method is not convenient for taking single crystal samples, and the crystals manually prepared after crystal sampling are prone to accumulation and interlacing.

Description

Technical Field

[0001] The invention relates to the technical field of fermentation sample detection, and in particular to a p-hydroxyphenylglycine fermentation enzyme sample detection device. Background Art

[0002] p-Hydroxyphenylglycine (D-HPG) is an important pharmaceutical fine chemical and a key intermediate for the synthesis of β-lactam semi-synthetic antibiotics. p-Hydroxyphenylglycine fermentation enzyme has important application value in the pharmaceutical field. The yield and quality of D-HPG can be improved by continuously optimizing the fermentation process. In the fermentation work by fermentation enzyme, the quality of p-Hydroxyphenylglycine crystals needs to be ensured. In the enzymatic reaction, the substrate refers to the substance acted by the enzyme. When the amount of substrate is small, the reaction speed will also slow down. As the substrate concentration increases, the reaction speed will increase accordingly. Usually, it is sent for inspection after manual sampling and observed under a microscope. However, the traditional manual sampling method is not convenient for real-time sampling and detection on the fermentation tank, which affects the timeliness of the detection. At the same time, each sampling will also affect the sealing of the tank. p-Hydroxyphenylglycine crystals will precipitate. Manual sampling is not standard enough, and it is not convenient to sample comprehensively at different precipitation heights. The substrate detection is not standard enough. At the same time, it is not convenient to take single crystal samples during sample detection. After crystal sampling, the crystals prepared manually are easy to pile up and interlace, which affects the staining and messy microscopic observation. Summary of the invention

[0003] The disclosed embodiment relates to a para-hydroxyphenylglycine fermentation enzyme sample detection device, whose single-layer suction sampling piece can facilitate the sampling of single crystals, and can prevent messy crystals from affecting microscopic observation while making staining more convenient. It can also play the role of liquid absorption, reducing the influence of fermentation liquid on staining, and solves the problem that the traditional manual sampling method is not convenient for taking single crystal samples, and the crystals manually prepared after crystal sampling are prone to stacking and interlacing.

[0004] The present invention provides a sample detection device for para-hydroxyphenylglycine fermentation enzyme, comprising a detection mounting part, on which a detection conveying part is mounted; a lifting conveying part is mounted on the detection conveying part; the lifting conveying part is used to take a crystal sample in a fermentation tank; a suction single-layer sampling part is mounted on the side of the detection conveying part; the suction single-layer sampling part is used to extract and take samples; a dyeing part is mounted on the detection mounting part; the dyeing part is used to dye the sample; a cleaning auxiliary part is fixedly mounted on the side of the detection conveying part; the cleaning auxiliary part is used to clean the lifting conveying part; a crystal sample storage part is mounted on the detection mounting part; the crystal sample storage part is used to collect crystal samples; the detection mounting part comprises: a detection flange plate, an observation mirror and a fill light, the detection flange plate is fixedly embedded with an observation mirror, and the observation mirror is a transparent lens; the detection flange plate is used to be mounted on the fermentation tank by bolts; a fill light is fixedly mounted inside the detection flange plate, and the observation mirror is aligned with the fill light.

[0005] In at least some embodiments, the suction single-layer sampling piece also includes: a dye adsorption sponge and an air jet tube, wherein the dye adsorption sponge is sleeved inside the suction tube; the dye adsorption sponge is used to absorb the dye; the air jet tube is connected to the suction pump; the air jet tube is fixedly sleeved on the side of the conveying mounting frame; the air jet tube is used to blow air above the rubber microporous belt; the air jet tube is aligned with the crystal discharge hole; and the air jet tube is located above the rubber microporous belt.

[0006] In at least some embodiments, the suction single-layer sampling piece includes: a suction pump, an air inlet pipe and a suction tube, the suction pump is fixedly mounted on the side of the conveying mounting frame; the air inlet pipe is fixedly mounted on the suction pump, and the suction tube is threadedly connected to the air inlet pipe; the end of the suction tube is fixedly mounted on the side of the conveying mounting frame; the end of the suction tube is connected to the airway; the end of the suction tube is aligned with the breathable sponge strip.

[0007] In at least some embodiments, the detection mounting component also includes: an electron microscope base, an electron microscope base is fixedly mounted on the side of the detection flange plate, and two through holes are provided on the electron microscope base; the two through holes on the electron microscope base are used to pass bolts to connect the electron microscope.

[0008] In at least some embodiments, the dyeing part includes: a dripping tube, a threaded connecting tube, a pushing screw and a piston, wherein the dripping tube is fixedly mounted on the detection flange plate; a threaded connecting tube is threadedly connected to the dripping tube; a pushing screw is threadedly connected to the threaded connecting tube, and a piston is fixedly mounted on the end of the pushing screw; the piston is sleeved on the threaded connecting tube; a handwheel is provided on the pushing screw; and the dripping tube is located above the rubber microporous belt.

[0009] In at least some embodiments, the cleaning auxiliary component includes: a cleaning column and a cleaning rubber scraper, wherein both ends of the cleaning column are fixedly mounted on the side of the conveying mounting frame; the cleaning column is located on the side of the rubber microporous belt; the cleaning column is used to stop and collect the rubber sheets; a cleaning rubber scraper is fixedly mounted on the inner side of the cleaning column, and the cleaning rubber scraper is attached to the side of the rubber microporous belt; the cleaning rubber scraper is used to scrape the cleaning rubber microporous belt.

[0010] In at least some embodiments, the lifting conveyor includes: a lifting conveyor belt, a rubber microporous belt, a spacing support block and a collecting rubber sheet, wherein the lifting conveyor belt is located on the outside of the conveying mounting frame; the lifting conveyor belt transmission sleeve is connected to two conveying wheels; the two sides of the rubber microporous belt are fixedly installed on the outside of the lifting conveyor belt; a circle of spacing support blocks is fixedly installed inside the rubber microporous belt, and a circle of spacing support blocks are respectively fixedly installed on the outside of the lifting conveyor belt; the outside of the rubber microporous belt is a microporous structure; a circle of collecting rubber sheets is fixedly installed on the rubber microporous belt, and the circle of collecting rubber sheets are respectively arc-shaped soft structures; a circle of through holes is provided on the side of the rubber microporous belt, and the circle of through holes on the side of the rubber microporous belt is aligned with the airway; the rubber microporous belt is used to adsorb parahydroxyphenylglycine crystals.

[0011] In at least some embodiments, the detection conveyor includes: a conveying mounting frame, an airway, a crystal discharge hole, a conveying wheel, a conveying motor and a breathable sponge strip, wherein the conveying mounting frame is fixedly mounted on the bottom of the detection flange plate; an airway is opened on the side of the conveying mounting frame; a crystal discharge hole is opened on the side of the conveying mounting frame; the crystal discharge hole is used to discharge the parahydroxyphenylglycine crystals blown out by the suction single-layer sampling piece; two conveying wheels are rotatably mounted on the conveying mounting frame; a conveying motor is fixedly mounted on the conveying mounting frame, and the output shaft of the conveying motor is fixedly mounted on the conveying wheel on the same side; a breathable sponge strip is embedded above the airway; the breathable sponge strip is used to isolate impurities.

[0012] In at least some embodiments, the crystal sample storage part further includes: an isolation net, and isolation nets are fixedly installed on both sides of the groove at the bottom of the sample storage slider; two isolation nets are located on both sides of the crystal discharge hole.

[0013] In at least some embodiments, the crystal sample storage part includes: a sample storage slider and a mounting bolt, the sample storage slider is slidably inserted on the detection flange plate; the sample storage slider is inserted with a mounting bolt, and the mounting bolt is threadedly connected to the detection flange plate; a groove is provided at the bottom of the sample storage slider.

[0014] The present invention provides a p-hydroxyphenylglycine fermentation enzyme sample detection device, which has the following beneficial effects:

[0015] The detection mounting part used in the present invention can be directly installed in the para-hydroxyphenylglycine fermentation tank to detect the reaction quality of the fermentation enzyme. There is no need to manually extract samples for inspection, and the detection is more direct. The structure can be directly docked and inspected through a microscope, which is more practical. At the same time, the structure does not affect the sealing of the tank body during sampling and inspection, which can improve the fermentation sealing effect and ensure the accuracy of the atmosphere in the para-hydroxyphenylglycine fermentation tank.

[0016] In addition, the lifting conveyor can be used in conjunction with the detection conveyor to facilitate comprehensive sampling. A circle of movable collecting rubber sheets can be used to collect crystallized samples at different heights. At the same time, this structure can help staff understand the thickness of the precipitation layer in the fermentation tank and the quality of the reaction. Cleaning auxiliary parts can be used to achieve automatic cleaning, ensuring continuous sampling and avoiding repeated testing.

[0017] In addition, the use of a single-layer suction sampling piece can ensure the stability of the hydroxyphenylglycine crystals during testing through negative pressure suction. The use of exhaust gas to blow air toward the rubber microporous belt can facilitate the removal of the upper layer of crystals except for a layer of crystals attached to the surface of the rubber microporous belt, making subsequent dyeing more convenient and reducing the amount of dye used. It also prevents the disorderly accumulation of crystals from affecting the observation of impurities under a microscope.

[0018] In addition, the use of the crystal sample storage part can facilitate the staff to carry out sample retention work, realize automatic retention of crystal samples in the fermentation tank, and use a rubber microporous belt to transport the crystals. The crystals blown off the surface when moving are collected. The crystal sampling is comprehensive, and the structure of the crystal sample storage part can ensure the sealing when the sample is retained and taken. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solution of the embodiment of the present invention, the drawings of the embodiment are briefly introduced below.

[0020] The drawings described below are only related to some embodiments of the present invention, but are not intended to limit the present invention.

[0021] In the attached picture:

[0022] Figure 1 The overall structure schematic diagram of a p-hydroxyphenylglycine fermentation enzyme sample detection device of the present application is shown;

[0023] Figure 2 A schematic diagram of the bottom structure of a p-hydroxyphenylglycine fermentation enzyme sample detection device of the present application is shown;

[0024] Figure 3 A cross-sectional view of the internal structure of a p-hydroxyphenylglycine fermentation enzyme sample detection device of the present application is shown;

[0025] Figure 4A schematic diagram showing the structure of the detection mounting member of the present application is shown;

[0026] Figure 5 A schematic diagram showing the structure of the detection conveyor of the present application is shown;

[0027] Figure 6 This application shows Figure 5 A magnified view of the structure of the middle B region;

[0028] Figure 7 A schematic diagram showing the lifting and conveying member structure of the present application is shown;

[0029] Figure 8 This application shows Figure 3 A magnified view of the structure of the middle C region;

[0030] Fig. 9 A cross-sectional view of the structure of the suction single-layer sampling piece of the present application is shown;

[0031] Fig.10 This application shows Fig. 9 A magnified view of the structure of the middle E region;

[0032] Fig.11 A schematic diagram showing the overall structure of the suction single-layer sampling piece of the present application is shown;

[0033] Fig.12 A schematic diagram of the dyeing piece structure of the present application is shown.

[0034] List of reference numerals:

[0035] 1. Detection installation parts; 101. Detection flange plate; 102. Observation mirror; 103. Fill light; 104. Electron microscope support; 2. Detection conveyor parts; 201. Conveyor mounting frame; 2011. Airway; 2012. Crystal discharge hole; 202. Conveyor wheel; 203. Conveyor motor; 204. Breathable sponge strip; 3. Lifting conveyor parts; 301. Lifting conveyor belt; 302. Rubber microporous belt; 3021. Interval support block; 303. Collecting rubber 1. Sampling piece; 4. Suction single-layer sampling piece; 401. Suction pump; 402. Air inlet pipe; 403. Suction pipe; 404. Dye adsorption sponge; 405. Jet pipe; 5. Dyeing piece; 501. Dropping tube; 502. Threaded connecting pipe; 503. Pushing screw; 5031. Piston; 6. Cleaning auxiliary piece; 601. Cleaning column; 602. Cleaning rubber scraper; 7. Crystal sample storage part; 701. Sample storage slider; 702. Mounting bolt; 703. Isolation net. DETAILED DESCRIPTION

[0036] In order to make the purpose, technical solution and advantages of the embodiment of the present invention clearer, the technical solution of the embodiment of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiment of the present invention. Obviously, the described embodiment is a part of the embodiment of the present invention, not all of the embodiments. Based on the described embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] Example 1: Please refer to Figures 1 to 12 :

[0038] The present invention proposes a sample detection device for p-hydroxyphenylglycine fermentation enzyme, comprising a detection mounting part 1, on which a detection conveying part 2 is mounted; on which a lifting conveying part 3 is mounted; the lifting conveying part 3 is used to take crystallized samples in a fermentation tank; a suction single-layer sampling part 4 is mounted on the side of the detection conveying part 2; the suction single-layer sampling part 4 is used to extract and take samples; a dyeing part 5 is mounted on the detection mounting part 1; the dyeing part 5 is used to dye the sample; a cleaning auxiliary part 6 is fixedly mounted on the side of the detection conveying part 2; the cleaning auxiliary part 6 is fixedly mounted on the side of the detection conveying part 2 The auxiliary component 6 is used to clean the lifting and conveying component 3; a crystal sample storage part 7 is installed on the detection mounting component 1; the crystal sample storage part 7 is used to collect crystal samples; the detection mounting component 1 includes: a detection flange plate 101, an observation mirror 102 and a fill light 103, and the detection flange plate 101 is fixedly embedded with an observation mirror 102, and the observation mirror 102 is a transparent lens; the detection flange plate 101 is used to be installed on the fermentation tank by bolts; a fill light 103 is fixedly installed inside the detection flange plate 101, and the observation mirror 102 is aligned with the fill light 103.

[0039] In the disclosed embodiment, the detection mounting part 1 also includes: an electron microscope support 104, an electron microscope support 104 is fixedly mounted on the side of the detection flange plate 101, and two through holes are provided on the electron microscope support 104; the two through holes on the electron microscope support 104 are used to pass bolts to connect the electron microscope; the detection conveying part 2 includes: a conveying mounting frame 201, an airway 2011, a crystal discharge hole 2012, a conveying wheel 202, a conveying motor 203 and a breathable sponge strip 204, the conveying mounting frame 201 is fixedly mounted on the bottom of the detection flange plate 101; an airway 2011 is opened on the side of the conveying mounting frame 201; a crystal discharge hole 2012 is opened on the side of the conveying mounting frame 201; the crystal discharge hole 2012 is used to discharge the para-hydroxyphenylglycine crystals blown out by the suction single-layer sampling part 4; two Conveying wheel 202; a conveying motor 203 is fixedly mounted on the conveying mounting frame 201, and the output shaft of the conveying motor 203 is fixedly mounted on the conveying wheel 202 on the same side; a breathable sponge strip 204 is embedded above the airway 2011; the breathable sponge strip 204 is used to isolate impurities; the detection mounting part 1 can be directly installed in the para-hydroxyphenylglycine fermentation tank to detect the reaction quality of the fermentation enzyme, without the need for tedious manual sampling and inspection, and the inspection is more direct. This structure can be directly docked and inspected with a microscope, which is more practical. At the same time, during the sampling and inspection process of this structure, the tank body sealing is not affected, ensuring the accuracy of the atmosphere in the para-hydroxyphenylglycine fermentation tank. The detection conveying part 2 used is directly extended into the tank body to ensure comprehensive inspection. The electron microscope lens can be aligned with the observation mirror 102, and then the fill light 103 can be turned on for fill light work, which is convenient for observation.

[0040] In the disclosed embodiment, the lifting conveyor 3 includes: a lifting conveyor belt 301, a rubber microporous belt 302, an interval support block 3021 and a collecting rubber sheet 303. The lifting conveyor belt 301 is located on the outside of the conveying mounting frame 201; the lifting conveyor belt 301 is transmission-sleeved on the two conveying wheels 202; both sides of the rubber microporous belt 302 are fixedly installed on the outside of the lifting conveyor belt 301; a circle of interval support blocks 3021 are fixedly installed inside the rubber microporous belt 302, and a circle of interval support blocks 3021 are respectively fixedly installed on the outside of the lifting conveyor belt 301; the outside of the rubber microporous belt 302 is a microporous structure; a circle of collecting rubber sheets 303 are fixedly installed on the rubber microporous belt 302, and a circle of collecting rubber sheets 303 are respectively It is an arc-shaped soft structure; a circle of through holes is provided on the side of the rubber microporous belt 302, and the circle of through holes on the side of the rubber microporous belt 302 is aligned with the airway 2011; the rubber microporous belt 302 is used to adsorb the p-hydroxyphenylglycine crystals; the cleaning auxiliary part 6 includes: a cleaning column 601 and a cleaning rubber scraper 602, and both ends of the cleaning column 601 are fixedly mounted on the side of the conveying mounting frame 201; the cleaning column 601 is located on the side of the rubber microporous belt 302; the cleaning column 601 is used to stop and collect the rubber sheet 303; a cleaning rubber scraper 602 is fixedly installed on the inner side of the cleaning column 601, and the cleaning rubber scraper 602 is attached to the side of the rubber microporous belt 302; the cleaning rubber scraper 602 is used to scrape the cleaning rubber microporous belt 302, and a lifting conveyor is used. 3 can cooperate with the detection conveying part 2 to facilitate comprehensive sampling. A circle of movable collecting rubber sheets 303 can be used to collect crystal samples of different heights, improve the comprehensiveness of sample detection, and ensure accurate detection. At the same time, this structure can facilitate the staff to understand the thickness of the sedimentation layer in the fermentation tank, facilitate the understanding of the reaction quality, and sample sampling is fast. This structure can be automatically cleaned with the cleaning auxiliary part 6 to ensure continuous sampling, ensure that the samples that have been detected are no longer sampled again, improve the quality of sample detection, and avoid repeated detection. This structure uses a circle of collecting rubber sheets 303 to collect samples without damaging the crystals, which improves the integrity of the crystals. The staff can pay attention to observe the starting point of the collecting rubber sheet 303. During dynamic transportation, crystallization begins to appear in the first collecting rubber sheet 303, so that the sample can form a sample sequence, which is more accurate. The sample sequence is detected during the catalytic process because the intervals of a circle of collecting rubber sheets 303 are the same, so the thickness of the crystal precipitation layer can be inferred, and the structure is more reasonable. The collecting rubber sheet 303 can collide with the cleaning column 601 during transportation, and the collecting rubber sheet 303 undergoes elastic deformation, that is, elastic swing to assist in removing crystals attached to the surface. The cleaning rubber scraper 602 is also attached to the surface of the rubber microporous belt 302 in real time for cleaning and scraping. In the enzymatic reaction, the substrate refers to the substance acted upon by the enzyme. When the amount of substrate is small, the reaction speed will also slow down. As the substrate concentration increases, the reaction speed will increase accordingly.

[0041] In the embodiment of the present disclosure, the suction single-layer sampling piece 4 includes: a suction pump 401, an air inlet pipe 402 and a suction pipe 403. The suction pump 401 is fixedly mounted on the side of the transport mounting frame 201; the air inlet pipe 402 is fixedly mounted on the suction pump 401, and the air inlet pipe 402 is threadedly connected with the suction pipe 403; the end of the suction pipe 403 is fixedly mounted on the side of the transport mounting frame 201; the end of the suction pipe 403 is connected to the airway 2011; the end of the suction pipe 403 is aligned with the breathable sponge strip 204; the suction single-layer sampling piece 4 also includes: a dye adsorption sponge 404 and an air jet pipe 405. The dye adsorption sponge 404 is sleeved inside the suction pipe 403; the dye adsorption sponge 404 is used to absorb The dye is collected; the jet pipe 405 is connected to the suction pump 401; the jet pipe 405 is fixedly sleeved on the side of the conveying mounting frame 201; the jet pipe 405 is used to blow air above the rubber microporous belt 302; the jet pipe 405 is aligned with the crystal discharge hole 2012; the jet pipe 405 is located above the rubber microporous belt 302; the dyeing part 5 includes: a dripping pipe 501, a threaded connecting pipe 502, a propulsion screw 503 and a piston 5031, the dripping pipe 501 is fixedly installed on the detection flange plate 101; the dripping pipe 501 is threadedly connected with the threaded connecting pipe 502; the threaded connecting pipe 502 is threadedly connected with the propulsion screw 503, and the end of the propulsion screw 503 is fixedly installed with the piston 5031 ; The piston 5031 is sleeved on the threaded connecting tube 502; a hand wheel is provided on the advancing screw rod 503; the dropper 501 is located above the rubber microporous belt 302, and the suction single-layer sampling piece 4 can ensure the stability of the p-hydroxyphenylglycine crystal during the detection by means of negative pressure suction. At the same time, the rubber microporous belt 302 is used to adsorb and prevent the attached crystals from falling off, and the exhaust gas is used to blow air into the rubber microporous belt 302, which can facilitate the blowing of the upper layer of crystals except for the layer of crystals attached to the surface of the rubber microporous belt 302, which can make subsequent dyeing more convenient and reduce the amount of dye used. At the same time, it can also avoid the disorderly accumulation of crystals affecting the observation of impurities under the microscope, and can be observed through a single crystal The sampling method prevents crystal accumulation, and the use of the dyeing part 5 is also convenient for quickly dyeing the crystals. The suction tube 403 used can perform suction in real time. On the one hand, it can prevent the crystals from falling off, and on the other hand, it can use negative pressure to recover a small amount of excess dye, which is quickly adsorbed and recovered by the dye adsorption sponge 404. When the crystal is under the observation mirror 102, the jet pipe 405 can spray to blow away the crystals that are not adsorbed by the rubber microporous belt 302. The dye that is not absorbed by the crystal and a small amount of fermentation liquid can be sucked into the dye adsorption sponge 404 for collection under the suction of the suction pump 401. The dye adsorption sponge 404 can be replaced by rotating and disassembling the air inlet pipe 402 and the suction tube 403.

[0042] Embodiment 2, on the basis of embodiment 1, the crystal sample storage part 7 comprises: a sample storage slider 701 and a mounting bolt 702, the sample storage slider 701 is slidably plugged into the detection flange plate 101; the mounting bolt 702 is plugged into the sample storage slider 701, and the mounting bolt 702 is threadedly connected to the detection flange plate 101; a groove is provided at the bottom of the sample storage slider 701; the crystal sample storage part 7 also comprises: an isolation net 703, and isolation nets 703 are fixedly installed on both sides of the groove at the bottom of the sample storage slider 701; two isolation nets 703 are located on both sides of the crystal discharge hole 2012. The use of the crystal sample storage part 7 can facilitate the staff to carry out sample retention work, and the structure is simple and can be In order to realize automatic retention of crystal samples in the fermentation tank and facilitate subsequent chemical experiments, the sampling method adopted by this structure utilizes the rubber microporous belt 302 to transport the crystals whose surfaces are blown off when the crystals move. The crystal sampling is comprehensive and the operation is simple. The sealing performance when the sample is taken can be ensured. The operation is simple and the atmosphere conditions in the fermentation tank can be maintained better. When taking out the sample, lift the sample storage slider 701 but do not completely pull it out from the detection flange plate 101. After the groove at the bottom of the sample storage slider 701 is exposed, use a medicine spoon or other sterile instrument to take the sample and retain the sample. In this process, the crystal impurity detection is not affected and it can be observed in real time through an electron microscope.

[0043] The working principle of this embodiment is as follows: first, after the flange tube is set on the para-hydroxyphenylglycine fermentation tank, the detection conveying member 2 can be inserted into the fermentation tank, and the detection flange plate 101 can be installed on the flange tube set on the para-hydroxyphenylglycine fermentation tank by bolts. The two through holes on the electron microscope support 104 are connected to the electron microscope through the bolts, and the lens is aligned with the observation mirror 102. Then, the fill light 103 can be turned on for fill light work, which is convenient for observation. The conveying motor 203 drives the conveying wheel 202 to rotate, which can drive the lifting conveyor belt 301 for conveying, and then drive a circle of collecting rubber sheets 303 for conveying. The arc structure of a circle of collecting rubber sheets 303 is used to pick up the crystal precipitation at its corresponding position. At the same time, the staff can pay attention to observe from which collecting rubber sheet 303 the crystal begins to appear when the conveying is started from the collecting rubber sheet 303. Because the intervals of a circle of collecting rubber sheets 303 are the same, the thickness of the crystal precipitation layer can be inferred, and the crystal samples of different heights can be collected, which improves the overall sample detection. The surface of the collecting rubber sheet 303 is ensured to be accurate in detection. At the same time, this structure can facilitate the staff to understand the thickness of the sedimentation layer in the fermentation tank and the reaction quality. As the collecting rubber sheet 303 is driven to move, the microporous structure of the rubber microporous belt 302 can facilitate the suction and adsorption of crystals by the suction single-layer sampling piece 4. The crystals are transported to the bottom of the observation mirror 102 for observation in turn. As the collecting rubber sheet 303 is transported and moved, the crystals are also observed and detected. The collecting rubber sheet 303 can collide with the cleaning column 601, and the collecting rubber sheet 303 undergoes elastic deformation, which can be elastically swung to assist in removing the crystals attached to the surface. The cleaning rubber scraper 602 is also attached to the surface of the rubber microporous belt 302 in real time for cleaning and scraping. The cleaning rubber scraper 602 is used to scrape the cleaning rubber microporous belt 302. This structure ensures continuous sampling, ensures that the samples that have been tested are no longer sampled again, improves the sample detection quality, and avoids repeated detection. This structure uses a circle of collecting rubber sheets 303 to transport the collected samples without damaging the crystals, thereby improving the crystal integrity.

[0044] After the suction pump 401 is started, the suction pipe 403 is suctioned in real time, and negative pressure is generated in the airway 2011 at this time. The circle of through holes on the side of the rubber microporous belt 302 is aligned with the airway 2011, and air flow can be conducted. As the crystal is transported to the bottom of the observation mirror 102, the surface of the rubber microporous belt 302 can absorb and collect the crystal particles on the rubber sheet 303 to avoid the disorderly accumulation of crystals affecting the observation of impurities under the microscope. The crystal is transported through the rubber microporous belt 302 for detection. When the crystal is under the observation mirror 102, the air jet pipe 405 sprays in real time to absorb the crystal particles on the upper layer that are not absorbed by the rubber. The crystals adsorbed by the microporous belt 302 are blown off. At this time, the screw rod 503 can be controlled to rotate and push the piston 5031, and the dye is dripped on the crystal sample through the dropper 501 for dyeing. It is convenient to blow off the upper layer of crystals except for the layer of crystals attached to the surface of the rubber microporous belt 302, which can be more convenient for subsequent dyeing and reduce the amount of dye. The excess dye not absorbed by the crystal can be sucked into the dye adsorption sponge 404 for collection under the suction of the suction pump 401. The dye adsorption sponge 404 can be replaced by rotating and disassembling the air inlet pipe 402 and the suction pipe 403. Component 5 is also convenient for quickly dyeing the crystals. The suction tube 403 used can perform suction in real time. On the one hand, it can prevent the crystals from falling off, and on the other hand, it can use negative pressure to recover a small amount of excess dye, which is quickly adsorbed and recovered by the dye adsorption sponge 404; when the jet tube 405 is jetting, the crystals that are not adsorbed by the rubber microporous belt 302 are blown away. When the crystals are blown to the crystal discharge hole 2012, they can pass through the crystal discharge hole 2012 and be blown into the groove provided at the bottom of the sample storage slider 701 for collection. The isolation net 703 plays the role of isolation and ventilation. When the sample needs to be taken out, the installation can be disassembled by rotating. Bolt 702, lift the sample storage slider 701 but do not completely remove it from the detection flange plate 101, which can ensure the sealing when taking the sample. The operation is simple and the atmosphere condition in the fermenter is better maintained. After the groove at the bottom of the sample storage slider 701 is exposed, use a sterile instrument such as a medicine spoon to take the sample and then retain the sample. The use of the crystal sample storage part 7 can facilitate the staff to perform sample retention work, and can realize automatic retention of crystal samples in the fermenter, which is convenient for subsequent chemical experiments and the like. At the same time, the crystal impurity detection is not affected in this process, and it can be observed in real time through an electron microscope.

[0045] In this article, there are a few points to note:

[0046] 1. The drawings of the embodiments of the present disclosure only involve structures related to the embodiments of the present disclosure, and other structures may refer to general designs.

[0047] 2. In the absence of conflict, the embodiments of the present disclosure and the features therein may be combined with each other to obtain new embodiments.

[0048] The above are only specific embodiments of the present disclosure, but the protection scope of the present disclosure is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed in the present disclosure, which should be included in the protection scope of the present disclosure. Therefore, the protection scope of the present disclosure should be based on the protection scope of the claims.

Claims

1. A p-hydroxyphenylglycine fermentation enzyme sample detection device, comprising a detection mounting member, on which a detection conveying member is mounted; characterized in that: The detection conveyor is equipped with a lifting conveyor; the lifting conveyor is used to take crystal samples in the fermentation tank; a suction single-layer sampling piece is installed on the side of the detection conveyor; the suction single-layer sampling piece is used to extract and take samples; a dyeing piece is installed on the detection mounting piece; the dyeing piece is used to dye the sample; a cleaning auxiliary piece is fixedly installed on the side of the detection conveyor; the cleaning auxiliary piece is used to clean the lifting conveyor; a crystal sample storage part is installed on the detection mounting piece; the crystal sample storage part is used to collect crystal samples; the detection mounting piece includes: a detection flange plate, an observation mirror and a fill light, an observation mirror is fixedly embedded on the detection flange plate, and the observation mirror is a transparent lens; the detection flange plate is used to be installed on the fermentation tank by bolts; a fill light is fixedly installed inside the detection flange plate, and the observation mirror is aligned with the fill light; the detection conveyor includes: a conveying mounting frame, an airway, a crystal A discharge hole, a conveying wheel, a conveying motor and a breathable sponge strip, the conveying mounting frame is fixedly mounted on the bottom of the detection flange plate; an airway is opened on the side of the conveying mounting frame; a crystal discharge hole is opened on the side of the conveying mounting frame; the crystal discharge hole is used to discharge the para-hydroxyphenylglycine crystals blown out by the suction single-layer sampling piece; two conveying wheels are rotatably mounted on the conveying mounting frame; a conveying motor is fixedly mounted on the conveying mounting frame, and the output shaft of the conveying motor is fixedly mounted on the conveying wheel on the same side; a breathable sponge strip is embedded above the airway; the breathable sponge strip is used to isolate impurities.

2. A p-hydroxyphenylglycine fermentation enzyme sample detection device according to claim 1, characterized in that: The detection mounting part also includes: an electron microscope support, the electron microscope support is fixedly mounted on the side of the detection flange plate, and two through holes are provided on the electron microscope support; the two through holes on the electron microscope support are used to pass bolts to connect the electron microscope.

3. A p-hydroxyphenylglycine fermentation enzyme sample detection device according to claim 1, characterized in that: The lifting conveyor comprises: a lifting conveyor belt, a rubber microporous belt, a spacing support block and a collecting rubber sheet, wherein the lifting conveyor belt is located on the outside of the conveying mounting frame; the lifting conveyor belt transmission sleeve is connected to two conveying wheels; both sides of the rubber microporous belt are fixedly mounted on the outside of the lifting conveyor belt; a circle of spacing support blocks is fixedly mounted inside the rubber microporous belt, and a circle of spacing support blocks are respectively fixedly mounted on the outside of the lifting conveyor belt; the outside of the rubber microporous belt is a microporous structure; a circle of collecting rubber sheets is fixedly mounted on the rubber microporous belt, and the circle of collecting rubber sheets are respectively arc-shaped soft structures; a circle of through holes is provided on the side of the rubber microporous belt, and the circle of through holes on the side of the rubber microporous belt is aligned with the airway; the rubber microporous belt is used to adsorb parahydroxyphenylglycine crystals.

4. A p-hydroxyphenylglycine fermentation enzyme sample detection device according to claim 3, characterized in that: The suction single-layer sampling piece includes: a suction pump, an air inlet pipe and a suction tube, the suction pump is fixedly installed on the side of the conveying mounting frame; the air inlet pipe is fixedly installed on the suction pump, and the suction tube is threadedly connected to the air inlet pipe; the end of the suction tube is fixedly installed on the side of the conveying mounting frame; the end of the suction tube is connected to the airway; the end of the suction tube is aligned with the breathable sponge strip.

5. A p-hydroxyphenylglycine fermentation enzyme sample detection device according to claim 4, characterized in that: The suction single-layer sampling piece also includes: a dye adsorption sponge and an air jet tube, wherein the dye adsorption sponge is sleeved inside the suction tube; the dye adsorption sponge is used to absorb the dye; the air jet tube is connected to the suction pump; the air jet tube is fixedly sleeved on the side of the conveying mounting frame; the air jet tube is used to blow air above the rubber microporous belt; the air jet tube is aligned with the crystal discharge hole; and the air jet tube is located above the rubber microporous belt.

6. A p-hydroxyphenylglycine fermentation enzyme sample detection device according to claim 3, characterized in that: The dyeing part includes: a dripping tube, a threaded connecting tube, a pushing screw and a piston. The dripping tube is fixedly installed on the detection flange plate; the dripping tube is threadedly connected with a threaded connecting tube; the threaded connecting tube is threadedly connected with a pushing screw, and a piston is fixedly installed at the end of the pushing screw; the piston is sleeved on the threaded connecting tube; the pushing screw is provided with a hand wheel; the dripping tube is located above the rubber microporous belt.

7. A p-hydroxyphenylglycine fermentation enzyme sample detection device according to claim 3, characterized in that: The cleaning auxiliary parts include: a cleaning column and a cleaning rubber scraper, wherein both ends of the cleaning column are fixedly mounted on the side of the conveying mounting frame; the cleaning column is located on the side of the rubber microporous belt; the cleaning column is used to stop and collect the rubber sheets; a cleaning rubber scraper is fixedly mounted on the inner side of the cleaning column, and the cleaning rubber scraper is attached to the side of the rubber microporous belt; the cleaning rubber scraper is used to scrape the cleaning rubber microporous belt.

8. A p-hydroxyphenylglycine fermentation enzyme sample detection device according to claim 1, characterized in that: The crystal sample storage part includes: a sample storage slider and a mounting bolt, the sample storage slider is slidably inserted on the detection flange plate; the sample storage slider is inserted with a mounting bolt, and the mounting bolt is threadedly connected to the detection flange plate; a groove is provided at the bottom of the sample storage slider.

9. A p-hydroxyphenylglycine fermentation enzyme sample detection device according to claim 8, characterized in that: The crystal sample storage part also includes: an isolation net, and isolation nets are fixedly installed on both sides of the groove at the bottom of the sample storage sliding block; two isolation nets are located on both sides of the crystal discharge hole.

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