Pet phagostimulant composition and pet food

Abstract

The invention relates to a pet phagostimulant composition with high palatability and high safety. The pet phagostimulant composition comprises animal meat, saccharides, strains, a bacteriostatic agent and a freshener. The invention also relates to a preparation method of the phagostimulant composition and an application of the phagostimulant composition for improving the palatability and safety of pet food. The invention also relates to a pet food comprising the pet phagostimulant composition.

Description

Technical Field

[0001] The present invention relates to the field of pet food and its additives, and specifically to a pet appetite attractant composition and pet food, in particular to a pet appetite attractant composition and pet food with high palatability and high safety. The present invention also relates to a method for producing the composition and a method for increasing the food intake / feeding rate of a pet, or increasing the palatability and / or safety of pet food by using the composition. Background Art

[0002] Pet attractants are additives that stimulate pets' sense of smell, taste, nerves, etc. to make pets take in more nutrients for health care. According to the differences in pet types, living environments, physiological habits, sensory organs and transmission media, and in combination with different types of pet food, pet attractants can be made into powder, liquid, paste and other material forms. As a technical difficulty in the upstream of the pet industry, pet attractants have good market prospects and development trends due to their complex technology and process and high profit margins. Since traditional pet food production usually has the problem of sacrificing palatability in order to improve nutritional value or production efficiency (for example, high-temperature puffing and extrusion processes will produce odors that reduce pet appetite), in recent years, almost all pet foods have added steps to improve the "palatability" of products during their production and upgrading. Moreover, in the process of changing pet food or changing the source of pet food, pets will not adapt, and the use of attractants to improve "palatability" can accelerate this adaptation process. In addition, for prescription pet foods or pet health products with therapeutic effects and pets with anorexia and picky eating characteristics, it is also necessary to improve the "palatability" of pet food in a timely manner.

[0003] As for pet attractants, they must ensure good attractant effects on pets, strictly implement the hygienic standards of feed additives, and must not have any other toxic side effects on pets. While increasing pets' food intake / feeding rate, increasing palatability and appetite, they must also ensure safety and be environmentally friendly. Moreover, they will not cause excessive production burdens.

[0004] Therefore, there is an urgent need in the art for a pet food attractant composition and pet food with high palatability and high safety, which can increase the pet's food intake / feeding rate, increase palatability and appetite, and have good safety, reduce the risk of toxin intake, and have a simple production process and save costs. To this end, the technical solution of the present invention is proposed. Summary of the invention

[0005] Specifically, the present invention solves the technical problems existing in the prior art through the following technical solutions:

[0006] 1. A pet appetite attractant composition, comprising:

[0007] (1) Animal meat, which is selected from the group consisting of raw animal meat, preferably raw animal offal, cooked animal meat and raw animal meat and bone paste;

[0008] (2) carbohydrates selected from the group consisting of monosaccharides, disaccharides, oligosaccharides and polysaccharides;

[0009] (3) a fermentation agent selected from the group consisting of lactobacillus, lactococci, pediococci, micrococci, staphylococci, yeasts and molds;

[0010] (4) an antibacterial agent selected from the group consisting of an inorganic acid or a salt thereof, an organic acid or a salt thereof, a fermentation product containing an inorganic acid, an organic acid or a salt thereof, and a preparation thereof; and

[0011] (5) A flavor enhancer selected from the group consisting of yeast extract, disodium inosinate, disodium guanylate, flavoring amino acids and their salts, and bouillon powder.

[0012] 2. The pet appetite attractant composition according to item 1, wherein the animal meat is selected from the group consisting of chicken, pork, beef, mutton, duck and goose, preferably chicken.

[0013] 3. The pet appetite attractant composition according to item 1 or 2, wherein the meat is sterilized at high temperature.

[0014] 4. The pet appetite attractant composition according to any one of items 1 to 3, wherein the sugar is selected from the group consisting of sucrose, glucose, maltose and fructose.

[0015] 5. A pet feeding attractant composition according to any one of items 1 to 4, wherein the lactobacillus is selected from the group consisting of Lactobacillus casei, Lactobacillus crispatus, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus johnsonii, Lactobacillus paracasei, Lactobacillus lactis, Lactobacillus plantarum, Lactobacillus curvifolius, Lactobacillus sakei, Lactobacillus rhamnosus and Lactobacillus salivarius; the lactococcus is selected from the group consisting of Lactococcus lactis, Lactococcus gasseri and Lactococcus cremoris; the genus Pediococcus is selected from the group consisting of : Pediococcus cerevisiae, Pediococcus acidilactici, Pediococcus pentosaceus and Pediococcus halophilus; the Micrococcus genus is selected from the following group: Micrococcus luteus and Micrococcus variants; the Staphylococcus genus is selected from the following group: Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus saprophyticus; the Saccharomyces cerevisiae is selected from the following group: Saccharomyces boulardii, Saccharomyces vitis vinifera, Saccharomyces rouxii, Saccharomyces rouxii, Saccharomyces pasteurianus, Saccharomyces paradoxica, Saccharomyces cerevisiae, Saccharomyces cerevisiae and Saccharomyces lutherii; the mold is selected from the following group: Aspergillus, Mucor, Fusarium and Penicillium.

[0016] 6. A pet appetite attractant composition according to any one of items 1 to 5, wherein the antibacterial agent is selected from the following group: acetic acid, acetate, diacetate, citric acid, citrate, lactic acid, lactate, nitrate, sorbic acid, potassium sorbate, benzoic acid, sodium benzoate, sucrose fermentation, glucose fermentation, vegetable juice fermentation, vinegar and vinegar powder.

[0017] 7. A pet appetite attractant composition according to any one of items 1 to 6, wherein the yeast extract is yeast extract or yeast extract powder; the flavor amino acid is selected from the following group: glutamic acid, aspartic acid, phenylalanine, alanine, glycine and tyrosine; the broth powder is selected from the following group: chicken broth powder, beef broth powder, pork broth powder, mutton broth powder and fish broth powder, and preferably the flavor enhancer is selected from the following group: glutamic acid, sodium glutamate, aspartic acid and sodium aspartate.

[0018] 8. The pet appetite attractant composition according to any one of items 1 to 7, wherein the content of the animal meat is 50-60% by weight of the composition.

[0019] 9. The pet appetite attractant composition according to any one of items 1 to 8, wherein the content of the sugar is 0.2-0.6% by weight of the composition.

[0020] 10. The pet appetite attractant composition according to any one of items 1 to 9, wherein the content of the leavening agent is 0.01-0.03% by weight of the composition.

[0021] 11. The pet appetite attractant composition according to any one of items 1 to 10, wherein the content of the antibacterial agent is 0.2-0.5% by weight of the composition.

[0022] 12. The pet appetite attractant composition according to any one of items 1 to 11, wherein the content of the flavor enhancer is 0.2-1% by weight of the composition.

[0023] 13. The pet attractant composition according to any one of items 1 to 12, further comprising a complex phosphate as a pH regulator, preferably the complex phosphate is a sodium salt, and more preferably the complex phosphate is selected from the following group: sodium tripolyphosphate, sodium hexametaphosphate, trisodium phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium acid pyrophosphate and disodium dihydrogen pyrophosphate.

[0024] 14. The pet appetite attractant composition according to item 13, wherein the content of the complex phosphate is 0.3-0.5% by weight of the composition.

[0025] 15. The pet appetite attractant composition according to any one of items 1 to 14, further comprising an edible colloid as a stabilizer, preferably the edible colloid is selected from the following group: carrageenan, xanthan gum, guar gum, agar, gelatin, sodium alginate, locust bean gum, konjac gum, collagen and hydrolyzed collagen.

[0026] 16. The pet appetite attractant composition according to item 15, wherein the content of the edible colloid is 0.2-0.5% by weight of the composition.

[0027] 17. A pet attractant composition according to any one of items 1 to 16, wherein the pet is selected from mammals or reptiles, preferably the pet is selected from the following group: cats, dogs, rabbits, guinea pigs, hamsters, hedgehogs, turtles and lizards.

[0028] 18. The pet appetite attractant composition according to any one of items 1 to 17, wherein the composition is used:

[0029] (i) increasing the amount and / or rate of pet food intake by pets to which the composition is added, compared to a control group to which the composition is not added, preferably by 70% or more;

[0030] (ii) increasing the palatability of pet food to which the composition is added, preferably by 70% or more, compared to a control group to which the composition is not added; and / or

[0031] (iii) the safety of pet food to which the composition is added is increased compared to a control group to which the composition is not added, preferably the risk of botulinum toxin and / or its toxin in pet food is reduced to zero.

[0032] 19. A method for producing the pet appetite attractant composition described in any one of items 1 to 18, comprising:

[0033] (i) chopping the animal meat and mixing it with water to prepare meat paste;

[0034] (ii) activating the starter culture, and adding the activated starter culture to the meat paste;

[0035] (iii) adding the sugar and flavor enhancer to the mixture of step (ii) and mixing;

[0036] (iv) fermenting at 28-43° C. for 6-12 hours;

[0037] (v) adding the antibacterial agent, mixing, and leaving at room temperature for 12-72 hours; and

[0038] (vi) Optional sterilization and / or filling.

[0039] 20. A method according to claim 19, wherein in step (i) the animal meat is chopped, treated at high temperature, and mixed with an equal amount of water.

[0040] 21. The method according to item 19 or 20, wherein in step (ii), the leavening agent is dissolved in 30-35°C water at a ratio of 1:10 and then allowed to stand for 0-2.5 hours, preferably for 1-1.5 hours at room temperature.

[0041] 22. The method according to any one of items 19 to 21, wherein in step (iv) the fermentation is carried out at 30-35°C for 8-10 hours.

[0042] 23. A method according to any one of items 19-22, wherein a complex phosphate is added before, during or after step (iv) to adjust the pH.

[0043] 24. The method according to any one of items 19 to 23, wherein the pH at the end of the fermentation in step (iv) is 4.7-5.7, preferably 4.8-5.0.

[0044] 25. The method according to any one of items 19 to 24, wherein in step (v) the mixture is placed at room temperature for 24 to 36 hours.

[0045] 26. The method according to any one of items 19 to 25, wherein the sterilization in step (vi) is high temperature sterilization, pasteurization or filtration sterilization.

[0046] 27. A method for increasing the food intake and / or food intake rate of a pet, comprising mixing the pet appetite attractant composition according to any one of items 1 to 18 with pet food and then feeding the mixture to the pet.

[0047] 28. The method according to item 27, further comprising measuring the amount and / or rate of intake of the pet food to which the composition is added, and comparing it with a control group to which the composition is not added.

[0048] 29. A method for increasing the palatability and / or safety of pet food, comprising mixing the pet appetite attractant composition according to any one of items 1 to 18 with pet food and then feeding the mixture to a pet.

[0049] 30. The method according to item 29, further comprising determining the palatability and / or safety of the pet food to which the composition is added, and comparing it with a control group to which the composition is not added.

[0050] 31. A pet food, comprising or consisting of the pet appetite attractant composition according to any one of items 1 to 18.

[0051] In order to make the technical solution of the present invention clearer, the present invention is further described in detail below in conjunction with specific implementation methods. DETAILED DESCRIPTION OF THE INVENTION

[0053] 1. Pet appetite attractant composition of the present invention

[0054] In one aspect, the present invention provides a pet appetite attractant composition comprising: (1) animal meat; (2) carbohydrates; (3) a fermentation agent; (4) an antibacterial agent; and (5) a flavor enhancer.

[0055] In one embodiment, the pet attractant composition of the present invention comprises animal meat, and the animal meat is selected from the following groups: raw animal meat, cooked animal meat and raw animal meat and bone paste. In one embodiment, the animal meat is selected from the following groups: chicken, pork, beef, mutton, duck, goose and fish. In one embodiment, the animal meat is chicken. In one embodiment, the animal meat is sterilized at high temperature (e.g., 100°C, 105°C, 110°C, 115°C or 120°C). In one embodiment, the raw animal meat is raw animal offal. In one embodiment, the raw animal offal is selected from the following groups: liver, lung, stomach, large intestine, small intestine, heart, spleen, bladder, gallbladder, pancreas and kidney. In one embodiment, the cooked animal meat is made by cooking raw animal meat. In one embodiment, the cooking is selected from the following groups: steaming, boiling, roasting and frying. In one embodiment, the meat and bone paste is made by cutting, mincing and / or grinding the bones and meat of animals.

[0056] In one embodiment, the pet attractant composition of the present invention comprises a carbohydrate selected from the group consisting of a monosaccharide, a disaccharide, an oligosaccharide (oligosaccharide) and a polysaccharide. In one embodiment, the monosaccharide is an aldose or a ketose. In one embodiment, the monosaccharide is selected from the group consisting of a triose, a tetrose, a pentose, a hexose and a heptose. In one embodiment, the monosaccharide is selected from the group consisting of aldose, erythrose, thulose, arabinose, ribose, xylose, lyxose, glucose, mannose, fructose and galactose. In one embodiment, the disaccharide is selected from the group consisting of sucrose, lactose, maltose and trehalose. In one embodiment, the oligosaccharide or oligosaccharide is a compound polymerized via 2-50, 2-30, 2-20 or 2-10 glycosidic bonds. In one embodiment, the oligosaccharide or oligosaccharide is selected from an N-glycosidic bond type or an O-glycosidic bond type. In one embodiment, the oligosaccharide or oligosaccharide is selected from the group consisting of maltooligosaccharides, isomaltooligosaccharides, cyclodextrins, fructooligosaccharides, panose, sucrose oligosaccharides, milk oligosaccharides, chitin oligosaccharides, soybean oligosaccharides, galactose oligosaccharides, fructose oligosaccharides and xylo-oligosaccharides. In one embodiment, the polysaccharide is a homopolysaccharide or a heteropolysaccharide. In one embodiment, the polysaccharide is selected from the group consisting of starch, cellulose, glycogen, chitin (chitin), inulin, agar, hyaluronic acid, chondroitin sulfate, dermatan sulfate, keratan sulfate, heparin and heparan sulfate. In one embodiment, the carbohydrate is selected from the group consisting of sucrose, glucose, maltose and fructose.

[0057] In one embodiment, the pet attractant composition of the present invention comprises a fermentation agent, which is a microorganism selected from the group consisting of Lactobacillus, Lactococcus, Pediococcus, Micrococcus, Staphylococcus, Saccharomyces and mold. In one embodiment, the Lactobacillus genus is selected from the group consisting of Lactobacillus casei, Lactobacillus crispatus, Lactobacillus delbrueckii lactis, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus johnsonii, Lactobacillus paracasei, Lactobacillus lactis, Lactobacillus plantarum, Lactobacillus curvatum, Lactobacillus sakei, Lactobacillus rhamnosus and Lactobacillus salivarius. In one embodiment, the Lactococcus genus is selected from the group consisting of Lactococcus lactis, Lactococcus gasseri and Lactococcus creamer. In one embodiment, the Pediococcus genus is selected from the group consisting of Pediococcus cerevisiae, Pediococcus acidilactici, Pediococcus pentosaceus and Pediococcus halophilus. In one embodiment, the Micrococcus is selected from the group consisting of Micrococcus luteus and Micrococcus variants. In one embodiment, the Staphylococcus is selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus saprophyticus. In one embodiment, the Saccharomyces is selected from the group consisting of Saccharomyces boulardii, Saccharomyces vinifera, Saccharomyces cerevisiae, Saccharomyces rouxii, Saccharomyces pasteurianus, Saccharomyces paradoxica, Saccharomyces cerevisiae, Saccharomyces cerevisiae and Saccharomyces lutherii. In one embodiment, the mold is selected from the group consisting of Aspergillus, Mucor, Fusarium and Penicillium.

[0058] In one embodiment, the pet attractant composition of the present invention comprises an antibacterial agent, and the antibacterial agent is selected from the following groups: inorganic acids or salts thereof, organic acids or salts thereof, fermentation products containing inorganic acids, organic acids or salts thereof, and their preparations. In one embodiment, the inorganic acid or salt thereof is nitrate. In one embodiment, the organic acid or salt thereof is selected from the following groups: acetic acid, acetate, diacetate, citric acid, citrate, lactic acid, lactate, sorbic acid, potassium sorbate, benzoic acid and sodium benzoate. In one embodiment, the fermentation product is selected from the following groups: sucrose fermentation, glucose fermentation, and vegetable juice fermentation. In one embodiment, the preparation is selected from vinegar and vinegar powder. In one embodiment, the antibacterial agent is selected from the following groups: sucrose fermentation and vinegar powder. In one embodiment, the sucrose fermentation (also known as fermented sugar) is a natural food ingredient, a natural substance made by fermenting food probiotics in white sugar and yeast extract, with unique ingredients such as organic acids, polypeptides, and proteins, and is widely used in various types of processed foods. Sucrose fermentation products can be used as seasonings to improve the quality of meat products and increase the freshness and flavor of food. At the same time, sucrose fermentation products can also effectively inhibit the growth of microorganisms and pathogenic bacteria to a certain extent, thereby extending the shelf life of food products, so it is also a natural preservative. In one embodiment, the vinegar powder is a solid food additive made from edible vinegar through processes such as neutralization, concentration and spray drying, which can not only enhance appetite, promote digestive juice secretion and gastrointestinal motility, but also inhibit the growth and reproduction of pathogenic bacteria.

[0059] In one embodiment, the pet appetite attractant composition of the present invention comprises a flavor enhancer, and the flavor enhancer is selected from the following group: yeast extract, disodium inosinate, disodium guanylate, flavor amino acids and their salts, and broth powder. In one embodiment, the yeast extract, also known as yeast extract, is the product after the yeast undergoes plasmolysis and complete autolysis using its own hydrolytic enzyme system, removing the cell wall and insoluble molecules. During the production process, the degree of hydrolysis of the yeast's own protein is strictly controlled to meet different application needs; finally, the yeast extract is concentrated into a semi-paste, paste, oil-encapsulated, microcapsule or micro-particle powdered product to meet different application needs. In one embodiment, the yeast extract is yeast extract or yeast extract powder. In one embodiment, the flavor amino acid is selected from the following group: glutamic acid, aspartic acid, phenylalanine, alanine, glycine and tyrosine. In one embodiment, the bouillon powder is selected from the group consisting of chicken bouillon powder, beef bouillon powder, pork bouillon powder, mutton bouillon powder and fish bouillon powder. In one embodiment, the flavor enhancer is selected from the group consisting of glutamic acid, sodium glutamate, aspartic acid and sodium aspartate.

[0060] In one embodiment, the animal meat is present in an amount of 30-80%, 30-75%, 30-70%, 30-65%, 30-60%, 35-80%, 35-75%, 35-70%, 35-65%, 35-60%, 40-80%, 40-75%, 40-70%, 40-65%, 40-60%, 45-80%, 45-75%, 45-70%, 45-65%, 45-60%, 50-80%, 50-75%, 50-70%, 50-65% or 50-60% by weight of the composition.

[0061] In one embodiment, the content of the sugar is 0.01-2%, 0.01-1.5%, 0.01-1%, 0.01-0.9%, 0.01-0.8%, 0.01-0.7%, 0.01-0.6%, 0.02-2%, 0.02-1.5%, 0.02-1%, 0.02-0.9%, 0.02-0.8%, 0.02-0.7%, 0.02-0.6%, 0.05-2%, 0.05-1.5%, 0.05-1%, 0.05-0.9%, 0.05-0.8% by weight of the composition , 0.05-0.7%, 0.05-0.6%, 0.08-2%, 0.08-1.5%, 0.08-1%, 0.08-0.9%, 0.08-0.8%, 0.08-0.7%, 0.08-0.6%, 0.1-2%, 0.1-1.5%, 0.1-1%, 0.1-0.9%, 0.1-0.8%, 0.1-0.7%, 0.1-0.6%, 0.2-2%, 0.2-1.5%, 0.2-1%, 0.2-0.9%, 0.2-0.8%, 0.2-0.7% or 0.2-0.6%.

[0062] In one embodiment, the leavening agent is present in an amount of 0.001-0.2%, 0.001-0.15%, 0.001-0.1%, 0.001-0.08%, 0.001-0.05%, 0.001-0.03%, 0.002-0.2%, 0.002-0.15%, 0.002-0.1%, 0.002-0.08%, 0.002-0.05%, 0.002-0.03%, 0.005-0.2%, 0.005- 0.15%, 0.005-0.1%, 0.005-0.08%, 0.005-0.05%, 0.005-0.03%, 0.008-0.2%, 0.008-0.15%, 0.008-0.1%, 0.008-0.08%, 0.008-0.05%, 0.008-0.03%, 0.01-0.2%, 0.01-0.15%, 0.01-0.1%, 0.01-0.08%, 0.01-0.05% or 0.01-0.03%.

[0063] In one embodiment, the content of the antibacterial agent is 0.01-2%, 0.01-1.5%, 0.01-1%, 0.01-0.9%, 0.01-0.8%, 0.01-0.7%, 0.01-0.6%, 0.01-0.5%, 0.02-2%, 0.02-1.5%, 0.02-1%, 0.02-0.9%, 0.02-0.8%, 0.02-0.7%, 0.02-0.6%, 0.02-0.5%, 0.05-2%, 0.05-1.5%, 0.05-1%, 0.05-0.9%, 0.05-0.8%, 0.05-0. 7%, 0.05-0.6%, 0.05-0.5%, 0.08-2%, 0.08-1.5%, 0.08-1%, 0.08-0.9%, 0.08-0.8%, 0.08-0.7%, 0.08-0.6%, 0.08-0.5%, 0.1-2%, 0.1-1.5%, 0.1-1%, 0.1-0.9%, 0.1-0.8%, 0.1-0.7%, 0.1-0.6%, 0.1-0.5%, 0.2-2%, 0.2-1.5%, 0.2-1%, 0.2-0.9%, 0.2-0.8%, 0.2-0.7%, 0.2-0.6% or 0.2-0.5%.

[0064] In one embodiment, the content of the flavor enhancer is 0.01-5%, 0.01-4.5%, 0.01-4%, 0.01-3.5%, 0.01-3%, 0.01-2.5%, 0.01-2%, 0.01-1.5%, 0.01-1%, 0.05-5%, 0.05-4.5%, 0.05-4%, 0.05-3.5%, 0.05-3%, 0.05-2.5%, 0. 0.05-2%, 0.05-1.5%, 0.05-1%, 0.1-5%, 0.1-4.5%, 0.1-4%, 0.1-3.5%, 0.1-3%, 0.1-2.5%, 0.1-2%, 0.1-1.5%, 0.1-1%, 0.2-5%, 0.2-4.5%, 0.2-4%, 0.2-3.5%, 0.2-3%, 0.2-2.5%, 0.2-2%, 0.2-1.5% or 0.2-1%.

[0065] In one embodiment, the pet appetite attractant composition of the present invention further comprises a composite phosphate as a pH regulator. In one embodiment, the composite phosphate is used to adjust the pH value of the fermentation product, so that the pH value of the fermentation product remains stable, which is conducive to the formation of a rich special flavor and can further enhance the appetite attracting effect. In one embodiment, the composite phosphate is a sodium salt. In one embodiment, the composite phosphate is selected from the following group: sodium tripolyphosphate, sodium hexametaphosphate, trisodium phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium acid pyrophosphate and disodium dihydrogen pyrophosphate.

[0066] In one embodiment, the content of the complex phosphate is 0.05-2%, 0.05-1.5%, 0.05-1%, 0.05-0.9%, 0.05-0.8%, 0.05-0.7%, 0.05-0.6%, 0.05-0.5%, 0.08-2%, 0.08-1.5%, 0.08-1%, 0.08-0.9%, 0.08-0.8%, 0.08-0.7%, 0.08-0.6%, 0.08-0.5%, 0.1-2%, 0.1 -1.5%, 0.1-1%, 0.1-0.9%, 0.1-0.8%, 0.1-0.7%, 0.1-0.6%, 0.1-0.5%, 0.2-2%, 0.2-1.5%, 0.2-1%, 0.2-0.9%, 0.2-0.8%, 0.2-0.7%, 0.2-0.6%, 0.2-0.5%, 0.3-2%, 0.3-1.5%, 0.3-1%, 0.3-0.9%, 0.3-0.8%, 0.3-0.7%, 0.3-0.6% or 0.3-0.5%.

[0067] In one embodiment, the pet attractant composition of the present invention further comprises an edible colloid as a stabilizer. In one embodiment, the edible colloid is used to improve the texture of the attractant composition, making the attractant composition thicker and smoother, preventing the composition from precipitating water, and improving product stability. In one embodiment, the edible colloid is selected from the following group: carrageenan, xanthan gum, guar gum, agar, gelatin, sodium alginate, locust bean gum, konjac gum, collagen and hydrolyzed collagen.

[0068] In one embodiment, the edible colloid is present in an amount of 0.05-2%, 0.05-1.5%, 0.05-1%, 0.05-0.9%, 0.05-0.8%, 0.05-0.7%, 0.05-0.6%, 0.05-0.5%, 0.08-2%, 0.08-1.5%, 0.08-1%, 0.08-0.9%, 0.08-0.8%, 0.08-0. 7%, 0.08-0.6%, 0.08-0.5%, 0.1-2%, 0.1-1.5%, 0.1-1%, 0.1-0.9%, 0.1-0.8%, 0.1-0.7%, 0.1-0.6%, 0.1-0.5%, 0.2-2%, 0.2-1.5%, 0.2-1%, 0.2-0.9%, 0.2-0.8%, 0.2-0.7%, 0.2-0.6% or 0.2-0.5%.

[0069] In one embodiment, the pet attractant composition is used for feeding to a pet, and the pet is selected from a mammal or a reptile, and preferably the pet is selected from the following group: cats, dogs, rabbits, guinea pigs, hamsters, hedgehogs, turtles and lizards. In one embodiment, the pet attractant composition is fed to a pet alone. In one embodiment, the pet attractant composition is added to pet food and fed to the pet together with the pet food. In one embodiment, the pet attractant composition is mixed with pet food and fed to the pet.

[0070] In one embodiment, the pet attractant composition increases the pet's food intake and / or intake rate for pet food added with the composition compared to a control group without the composition. In one embodiment, the pet's food intake and / or intake rate for pet food added with the composition increases by more than 5%, more than 10%, more than 15%, more than 20%, more than 25%, more than 30%, more than 35%, more than 40%, more than 45%, more than 50%, more than 55%, more than 60%, more than 65% or more than 70% compared to a control group without the composition.

[0071] In one embodiment, the pet appetite attractant composition increases the palatability of the pet food to which the composition is added compared to a control group to which the composition is not added. In one embodiment, the palatability of the pet food to which the composition is added increases by more than 5%, more than 10%, more than 15%, more than 20%, more than 25%, more than 30%, more than 35%, more than 40%, more than 45%, more than 50%, more than 55%, more than 60%, more than 65%, or more than 70% compared to a control group to which the composition is not added.

[0072] In one embodiment, the pet appetite attractant composition increases the safety of pet food to which the composition is added compared to a control group to which the composition is not added. In one embodiment, the pet food to which the composition is added reduces the risk of botulinum toxin and / or its toxins therein to zero.

[0073] 2. Method for producing the pet appetite attractant composition of the present invention

[0074] In one aspect, the present invention also provides a method for producing the pet appetite attractant composition of the present invention, which comprises: (i) pureeing the animal meat; (ii) activating the fermentation agent and adding the activated fermentation agent to the puree; (iii) adding the sugar and flavor enhancer to the mixture of step (ii) and mixing; (iv) fermenting; (v) adding the antibacterial agent, mixing and leaving for a period of time.

[0075] In one embodiment, in the step (i), the animal meat is chopped and mixed with water to make a puree. In one embodiment, in the step (i), the animal meat is chopped and mixed with an equal amount of water to make a puree. In one embodiment, in the step (i), the animal meat is chopped, high-temperature treated, and mixed with an equal amount of water to make a puree. In one embodiment, in the step (ii), the fermentation agent is dissolved in water, left for a period of time and then added to the puree. In one embodiment, in the step (ii), the fermentation agent is dissolved in water at 15-42°C, 20-40°C, 25-37°C or 30-35°C in a ratio of 10:1, 5:1, 2:1, 1:1, 1:2, 1:5 or 1:10, and then left for 0-2.5 hours, 0.5-2.2 hours, 0.7-2.0 hours, 0.8-1.8 hours, 0.9-1.6 hours, or preferably left at room temperature for 1-1.5 hours. In one embodiment, in step (iv), the fermentation is carried out at 20-50° C., 22-48° C., 25-46° C., 26-45° C., 27-44° C., 28-43° C., 29-42° C. or 30-37° C. for 4-24 hours, 4.5-20 hours, 5-18 hours, 5.5-15 hours, 6-12 or 8-10 hours. In one embodiment, the fermentation is carried out at 30-35° C. for 8-10 hours. In one embodiment, complex phosphates are added before, during or after step (iv) to adjust the pH.

[0076] In one embodiment, the pH at the end of fermentation in step (iv) is 4.2-6.0, 4.3-5.8, 4.4-5.6, 4.5-5.5, 4.6-5.4, 4.7-5.3 or 4.8-5.2. In one embodiment, the pH at the end of fermentation in step (iv) is 4.8-5.0. In one embodiment, the antibacterial agent is added and mixed in step (v) and then placed at room temperature for 12-72 hours, 14-70 hours, 15-65 hours, 16-60 hours, 17-55 hours, 18-50 hours, 19-45 hours, 20-40 hours, 21-39 hours, 22-38 hours, 23-37 hours or 24-36 hours. In one embodiment, the method for producing the pet attractant composition of the present invention further includes (vi) sterilization and / or filling. In one embodiment, the sterilization is selected from the group consisting of high temperature sterilization, pasteurization or filtration sterilization.

[0077] 3. Methods to increase your pet’s food intake and / or rate of food intake

[0078] In one aspect, the present invention also provides a method for increasing the food intake and / or food intake rate of a pet, comprising: feeding the pet attractant composition of the present invention to the pet. In one embodiment, the method comprises adding the pet attractant composition of the present invention to pet food and then feeding it to the pet. In one embodiment, the method comprises mixing the pet attractant composition of the present invention with pet food and then feeding it to the pet. In one embodiment, the method comprises measuring the food intake and / or food intake rate of the pet for the pet food to which the composition is added, and comparing it with a control group to which the composition is not added.

[0079] 4. Methods for increasing the palatability and / or safety of pet food

[0080] In one aspect, the present invention also provides a method for increasing the palatability and / or safety of pet food, comprising: feeding the pet attractant composition of the present invention to the pet. In one embodiment, the method comprises adding the pet attractant composition of the present invention to pet food and feeding it to the pet. In one embodiment, the method comprises mixing the pet attractant composition of the present invention with pet food and feeding it to the pet. In one embodiment, the method comprises determining the palatability and / or safety of the pet food to which the composition is added, and comparing it with a control group to which the composition is not added. In one embodiment, the increase in palatability is characterized by Δ first bite preference or Δ feeding rate.

[0081] In one embodiment, the calculation formula of Δ first bite preference is as follows:

[0082] (Number of pets in the first bite preference experimental group / total number of pets * 100%) / (Number of pets in the first bite preference control group / total number of pets * 100%)

[0083] In one embodiment, the calculation formula of the Δfeeding rate is as follows:

[0084] (Feeding rate of experimental group - feeding rate of control group) / feeding rate of control group * 100%

[0085] In one embodiment, the increase in safety is characterized by the detection of Clostridium botulinum and / or its toxins and the changes in the pet's feeding status, feces status and mental state relative to the control group.

[0086] 5. Pet food of the present invention

[0087] In one aspect, the present invention also provides a pet food containing the pet attractant composition of the present invention. In one embodiment, the pet attractant composition of the present invention accounts for 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% of the pet food (by weight). In one embodiment, the pet food of the present invention consists of the pet attractant composition of the present invention.

[0088] 6. Advantages of the present invention

[0089] There are two common methods for preparing pet appetite attractants on the market: one is to prepare by enzymatic hydrolysis and Maillard reaction, which requires many raw materials, has a complex process, has large batch differences, and has an unstable appetite attractant effect; the other is to simply add yeast extract, which has a better appetite attractant effect, but is expensive and difficult to promote. Compared with the above two methods, the appetite attractant produced by the present invention has the following advantages:

[0090] (1) The method of the present invention adopts a fermentation method, which is different from the above two attractant preparation processes. Compared with the enzymatic hydrolysis and Maillard reaction processes, the process is simple and the production efficiency is high. The quality of the attractant obtained is stable and the attractant effect is good;

[0091] (2) Compared with the method of simply adding yeast extract, the amount of yeast extract added is significantly reduced, which greatly reduces the production cost. In addition, the appetite attractant of the present invention not only has the umami taste of yeast extract, but also has the meaty flavor produced by fermentation. The two flavors are further integrated to give the product a unique flavor, and the appetite attracting effect is further improved. Therefore, the composition of the present invention is a highly palatable product;

[0092] (3) The Maillard reaction is carried out in a reactor. By heating for a long time, a complex reaction occurs between sugars and proteins, amino acids, peptides and other substances, and the reactants have a meaty aroma and are appetizing. If umami substances such as yeast extract are directly added to the reactor in order to obtain both the umami taste of yeast extract and the meaty flavor of enzymatic hydrolysis, since the main components of yeast extract are amino acids and peptides, these substances will directly participate in the Maillard reaction, and ultimately only produce meaty flavor, but it is difficult to present the umami taste of yeast extract itself;

[0093] (4) The present invention uses high temperature sterilization to extend the fermentation time to 15 days, eliminate the bad smell produced by harmful bacteria, and further improve the palatability; and

[0094] (5) The present invention controls the pH value of the fermentation to 4.8-5.0, and the obtained pet appetite attractant composition has better flavor and better palatability. DETAILED DESCRIPTION

[0095] The present invention will be further described below in conjunction with specific embodiments, but these specific embodiments cannot be understood as limiting the scope of protection of the present invention. Those skilled in the art may make various changes or modifications to these specific embodiments without departing from the scope of the technical solution of the present invention, and the changed and modified embodiments still fall within the scope of protection of the present invention.

[0096] All chemicals or products used in the examples are commercially available.

[0097] Example 1. Appetite attractant composition of the present invention

[0098] 1.1 The pet appetite attractant composition I of the present invention comprises:

[0099] A raw meat mixture containing raw chicken breast, cooked chicken breast and chicken liver, with an added amount of 55%;

[0100] Sucrose, added at 0.25%;

[0101] A mixture of Lactobacillus lactis, Lactobacillus plantarum and Lactobacillus casei, added at 0.03%;

[0102] Vinegar powder, added at 0.3%

[0103] Yeast extract and monosodium glutamate, added at 0.4%.

[0104] 1.2 The pet appetite attractant composition II of the present invention comprises:

[0105] High temperature sterilized raw meat mixture, added in an amount of 50%;

[0106] Glucose, added at 0.5%;

[0107] A mixture of Lactobacillus lactis, Lactobacillus plantarum, Lactobacillus casei, Pediococcus acidilactici, Pediococcus pentosaceus and Saccharomyces cerevisiae, added at 0.03%;

[0108] Sucrose fermentation product, added at 0.5%;

[0109] A mixture of yeast extract, monosodium glutamate and chicken bouillon powder; added at 1%;

[0110] Compound phosphate: sodium tripolyphosphate and sodium pyrophosphate; the addition amount is 0.5%;

[0111] Guar gum, added at 0.35%.

[0112] 1.3 The specific preparation process of the pet appetite attractant compositions I and II of the present invention is as follows:

[0113] 1) Chop raw meat including raw chicken breast, cooked chicken breast, chicken liver, etc. with a chopper (optionally high temperature treatment at 100° C. for 15 minutes), and then add an equal amount of water to make meat paste;

[0114] 2) Activation of starter (bacteria): dilute the bacteria with 30-35°C pure water at a ratio of 1:10, and place at room temperature for 1-1.5 hours after fully dissolved for later use;

[0115] 3) Add the activated bacteria to the prepared meat paste and mix well;

[0116] 4) adding sugars together with yeast extract, sodium glutamate and other flavor enhancers to the meat paste of the mixed strains and further mixing;

[0117] 5) Fermentation: temperature 30-35°C, time 8-10 hours, optionally adding composite phosphate to achieve a pH value of 4.8-5.0 at the end of fermentation;

[0118] 6) adding antibacterial agent (and optional edible colloid) and mixing;

[0119] 7) Post-ripening: Cool and place for 24-48 hours;

[0120] 8) Sterilize and fill.

[0121] Example 2. Palatability of the Appetite Composition of the Present Invention

[0122] 2.1 The palatability of the attractant composition can be determined by testing the first bite preference of the pet using the double bowl method. The test process and results are as follows:

[0123] Ten cats aged 2-3 years in good health were selected and divided into five groups. Two food bowls of the same material and size were provided to each group. 30 grams of pet food was placed in each food bowl. The control group was cat food without the attractant composition, and the experimental group 1 was cat food mixed with the attractant composition I. The experimental group 2 was cat food mixed with commercial attractants. The cats were fed once on time at 2:00 p.m. every day, and the first bite preference choice of each cat was recorded.

[0124] Table 1. First bite preference test

[0125]

[0126] It can be seen that the number of cats with first bite preference in experimental group 1 is significantly better than that in the control group and experimental group 2. The Δ first bite preference value of experimental group 1 is calculated to be 88%-12%=76%, while the Δ first bite preference value of experimental group 2 is 36%-12%=24%.

[0127] 2.2 The palatability of the attractant composition can also be determined by testing the feeding rate of pets using the double bowl method. The test process and results are as follows:

[0128] 10 small dogs in good health and aged 2-3 years were selected and divided into five groups. Two food bowls of the same material and size were provided to each group. 100 grams of pet food was placed in each food bowl. The control group was dog food without the attractant composition, and the experimental group 1 was dog food mixed with the attractant composition II. The experimental group 2 was dog food mixed with commercial attractants. The dogs were fed once on time at 2:00 p.m. every day. The average remaining amount of dog food of the 10 dogs was weighed and recorded. The feeding rate could be obtained by comparing the consumed dog food with the original dog food.

[0129] Table 2. Feeding rate test

[0130]

[0131] It can be seen that the total average feeding rate of the experimental group was significantly higher than that of the control group, about 1.7 times that of the control group, and the total Δ feeding rate value was 67.3%, while the total average feeding rate of commercially available pet attractants was only slightly higher than that of the control group, and the total Δ feeding rate was only 5.4%.

[0132] Therefore, the fermented product appetite attractant of the present invention can significantly increase the palatability of pet food relative to both the control group and the commercially available appetite attractant.

[0133] Example 3. Safety of the food attractant composition of the present invention

[0134] During the fermentation process of meat, there is a risk of botulinum toxin and / or its toxins due to problems such as the quality of the raw meat, which may cause food poisoning. The present invention can inhibit the production of botulinum toxin and / or its toxins by adding an antibacterial agent to a mixture of sucrose fermentation product and vinegar powder, thereby increasing the safety of the attractant composition of the present invention.

[0135] 3.1 In vitro verification of safety

[0136] The attractant composition I without adding an antibacterial agent (control group 1), the attractant composition I of the present invention (experimental group 1), the attractant composition II of the present invention (experimental group 2), and the commercial attractant (experimental group 3, whose shelf life is at least more than 30 days) were left to stand at room temperature in a dry environment for 30 days. After 30 days, samples were taken to detect botulinum toxin and botulinum toxin, and the results are shown in Table 3.

[0137] Table 3. Detection results of Clostridium botulinum and its toxins

[0138] Group Botulinum toxin test results Botulinum toxin test results Control group 1 Checkout Checkout Experimental Group 1 Not detected Not detected Experimental Group 2 Not detected Not detected Experimental Group 3 Not detected Not detected

[0139] 3.2 In vivo verification of safety

[0140] Since no botulinum toxin and its toxin were detected in the attractant composition of the present invention (experimental groups 1-2) and the commercially available attractant (experimental group 3), they can be used for further in vivo safety verification experiments. The process and results of the experiments are as follows:

[0141] 30 cats in good health and aged 2-3 years were selected and randomly divided into 3 groups, 10 in each group. The cats were fed with the attractant composition I of the present invention (experimental group 1), the attractant composition I of the present invention (experimental group 2) and the commercially available attractant (experimental group 3) for 30 days respectively. In 30 days, each group randomly sampled 100 times for the cats' feeding status (number of times the feeding rate decreased by more than 15% / 100 times), fecal status (number of times soft stool or diarrhea / 100 times) and mental state (number of times of mental depression (including but not limited to not liking to exercise, curling up, reduced interest and lethargy) / 100 times). The experimental results are shown in Table 4.

[0142] Table 4. Safety in vivo validation results

[0143] Group Feeding status Stool status Mental state Experimental Group 1 1 / 100 1 / 100 2 / 100 Experimental Group 2 2 / 100 2 / 100 1 / 100 Experimental Group 3 9 / 100 6 / 100 11 / 100

[0144] The results showed that the feeding status, feces status and mental state of the pets in experimental group 1-2 were significantly better than those in experimental group 3. This proves that the antibacterial agent and food attractant composition of the present invention has good antibacterial effect and safety in addition to its unique flavor and excellent palatability, providing a reliable guarantee for the physical health of pets.

[0145] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the specific implementation of the present invention is described above, it should be understood by those skilled in the art that this is only an example, and the protection scope of the present invention is defined by the attached claims. Those skilled in the art may make various changes or modifications to the technical contents disclosed above without departing from the scope of the technical solution of the present invention, and these changes and modifications all fall within the protection scope of the present invention.

Claims

1. A pet appetite attractant composition, comprising: (1) Animal meat, which is selected from the group consisting of raw animal meat, preferably raw animal offal, cooked animal meat and raw animal meat and bone paste; (2) carbohydrates selected from the group consisting of monosaccharides, disaccharides, oligosaccharides and polysaccharides; (3) a fermentation agent selected from the group consisting of lactobacillus, lactococci, pediococci, micrococci, staphylococci, yeasts and molds; (4) an antibacterial agent selected from the group consisting of an inorganic acid or a salt thereof, an organic acid or a salt thereof, a fermentation product containing an inorganic acid, an organic acid or a salt thereof, and a preparation thereof; (5) A flavor enhancer selected from the group consisting of yeast extract, disodium inosinate, disodium guanylate, flavoring amino acids and their salts, and bouillon powder.

2. The pet appetite attractant composition according to claim 1, wherein the animal meat is selected from the group consisting of chicken, pork, beef, mutton, duck, goose and fish, preferably chicken.

3. The pet appetite attractant composition according to claim 1 or 2, wherein the meat is sterilized at high temperature.

4. The pet appetite attractant composition according to any one of claims 1 to 3, wherein the sugar is selected from the group consisting of sucrose, glucose, maltose and fructose.

5. The pet feeding attractant composition according to any one of claims 1 to 4, wherein the lactobacillus is selected from the group consisting of Lactobacillus casei, Lactobacillus crispatus, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus johnsonii, Lactobacillus paracasei, Lactobacillus lactis, Lactobacillus plantarum, Lactobacillus curvifolius, Lactobacillus sakei, Lactobacillus rhamnosus and Lactobacillus salivarius; the lactococcus is selected from the group consisting of Lactococcus lactis, Lactococcus gasseri and Lactococcus cremoris; the pediococcus is selected from the group consisting of The present invention relates to a method for preparing the present invention which is preferably selected from the group consisting of: Pediococcus cerevisiae, Pediococcus acidilactici, Pediococcus pentosaceus and Pediococcus halophilus; the genus Micrococcus is selected from the group consisting of: Micrococcus luteus and Micrococcus variants; the genus Staphylococcus is selected from the group consisting of: Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus saprophyticus; the genus Saccharomyces is selected from the group consisting of: Saccharomyces boulardii, Saccharomyces vitis vinifera, Saccharomyces rouxii, Saccharomyces pasteurianus, Saccharomyces paradoxica, Saccharomyces cerevisiae, Saccharomyces cerevisiae and Saccharomyces lutherii; the mold is selected from the group consisting of: Aspergillus, Mucor, Fusarium and Penicillium.

6. The pet appetite attractant composition according to any one of claims 1-5, wherein the antibacterial agent is selected from the following group: acetic acid, acetate, diacetate, citric acid, citrate, lactic acid, lactate, nitrate, sorbic acid, potassium sorbate, benzoic acid, sodium benzoate, sucrose fermentation product, glucose fermentation product, vegetable juice fermentation product, vinegar and vinegar powder.

7. The pet appetite attractant composition according to any one of claims 1-6, wherein the yeast extract is yeast extract or yeast extract powder; the flavor amino acid is selected from the following group: glutamic acid, aspartic acid, phenylalanine, alanine, glycine and tyrosine; the broth powder is selected from the following group: chicken broth powder, beef broth powder, pork broth powder, mutton broth powder and fish broth powder, and preferably the flavor enhancer is selected from the following group: glutamic acid, sodium glutamate, aspartic acid and sodium aspartate.

8. The pet appetite attractant composition according to any one of claims 1 to 7, wherein: (i) the animal meat content is 50-60% by weight of the composition; and / or (ii) the sugar content is 0.2-0.6% by weight of the composition; and / or (iii) the fermenting agent is present in an amount of 0.01-0.03% by weight of the composition; and / or (iv) the antibacterial agent is present in an amount of 0.2-0.5% by weight of the composition; and / or (v) The content of the flavor enhancer is 0.2-1% by weight of the composition.

9. The pet appetite attractant composition according to any one of claims 1-8, further comprising a complex phosphate as a pH regulator, preferably the complex phosphate is a sodium salt, more preferably the complex phosphate is selected from the group consisting of sodium tripolyphosphate, sodium hexametaphosphate, trisodium phosphate, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium acid pyrophosphate and disodium dihydrogen pyrophosphate, more preferably the content of the complex phosphate is 0.3-0.5% by weight of the composition.

10. The pet appetite attractant composition according to any one of claims 1-9, further comprising an edible colloid as a stabilizer, preferably the edible colloid is selected from the following group: carrageenan, xanthan gum, guar gum, agar, gelatin, sodium alginate, locust bean gum, konjac gum, collagen and hydrolyzed collagen, more preferably the content of the edible colloid is 0.2-0.5% by weight of the composition.

11. The pet attractant composition according to any one of claims 1 to 10, wherein the pet is selected from mammals or reptiles, preferably the pet is selected from the following group: cats, dogs, rabbits, guinea pigs, hamsters, hedgehogs, turtles and lizards.

12. The pet appetite attractant composition according to any one of claims 1 to 11, wherein the composition is used: (i) increasing the amount and / or rate of pet food intake by pets to which the composition is added, compared to a control group to which the composition is not added, preferably by 70% or more; (ii) increasing the palatability of pet food to which the composition is added, preferably by 70% or more, compared to a control group to which the composition is not added; and / or (iii) the safety of pet food to which the composition is added is increased compared to a control group to which the composition is not added, preferably the risk of botulinum toxin and / or its toxin in pet food is reduced to zero.

13. A method for producing the pet appetite attractant composition according to any one of claims 1 to 12, comprising: (i) chopping the animal meat and mixing it with water to prepare meat paste; (ii) activating the starter culture, and adding the activated starter culture to the meat paste; (iii) adding the sugar and flavor enhancer to the mixture of step (ii) and mixing; (iv) fermenting at 28-43° C. for 6-12 hours; (v) adding the antibacterial agent, mixing, and leaving at room temperature for 12-72 hours; and (vi) Optional sterilization and / or filling.

14. The method according to claim 13, wherein: (a) in step (i), the animal meat is chopped, subjected to high temperature treatment, and mixed with an equal amount of water; and / or (b) dissolving the fermentation agent in step (ii) with water at 30-35° C. at a ratio of 1:10, and then leaving the mixture for 0-2.5 hours, preferably for 1-1.5 hours at room temperature; and / or (c) fermenting at 30-35° C. for 8-10 hours in step (iv); and / or (d) adding a complex phosphate to adjust the pH before, during or after step (iv); and / or (e) the pH at the end of the fermentation in step (iv) is 4.7-5.7, preferably 4.8-5.0; and / or (f) in step (v) leaving at room temperature for 24-36 hours; and / or (g) In step (vi), the sterilization is high temperature sterilization, pasteurization or filtration sterilization.

15. A method for increasing the food intake and / or food intake rate of a pet, comprising mixing the pet appetite attractant composition according to any one of claims 1 to 12 with pet food and then feeding the mixture to the pet.

16. The method according to claim 15, further comprising measuring the amount and / or rate of intake of the pet food to which the composition is added, and comparing the amount and / or rate of intake of the pet food to which the composition is not added with a control group.

17. A method for increasing the palatability and / or safety of pet food, comprising mixing the pet appetite attractant composition according to any one of claims 1 to 12 with pet food and then feeding the mixture to a pet.

18. The method of claim 17, further comprising determining the palatability and / or safety of the pet food to which the composition is added, and comparing it with a control group to which the composition is not added.

19. A pet food, comprising or consisting of the pet appetite attractant composition according to any one of claims 1 to 12.

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