A gas-liquid exchange structure, a culture device and a culture method

Abstract

The present invention discloses a gas-liquid exchange structure, a culture device and a culture method, which belong to the field of microalgae culture technology. The gas-liquid exchange structure includes a gas exchange structure and a liquid exchange structure, the gas exchange structure is located inside the liquid exchange structure, the upper part of the liquid exchange structure is located inside the culture box, the liquid exchange structure is sealed and rotatably connected to the culture box, and the lower part of the liquid exchange structure is located outside the culture box. The gas-liquid exchange structure, the culture device and the culture method described in the present invention can solve the problem of high cost of existing microalgae culture and harvesting, and facilitate the study of the influence of different external environments on microalgae culture.

Description

Technical Field

[0001] The present invention relates to the technical field of microalgae cultivation, and in particular to a gas-liquid exchange structure, a cultivation device and a cultivation method. Background Art

[0002] Currently, the economic success of microalgae farming depends on the ability to overcome production cost barriers. These barriers include high water costs and water recycling costs, nutrient and recycling costs, methods for efficient use of carbon dioxide, and microalgae harvesting costs. It is urgent to develop microalgae cultivation and microalgae harvesting technologies with low manufacturing and operating costs.

[0003] Microalgae are cultivated in an incubator. Light and temperature have a relatively large impact on the growth of microalgae. Studying the light and temperature environment suitable for the growth of microalgae is of great significance to improving the reproduction of microalgae. Summary of the invention

[0004] The purpose of the present invention is to provide a gas-liquid exchange structure, a culture device and a culture method to solve the problem of high cost of existing microalgae culture and harvesting, and to facilitate the study of the influence of different external environments on microalgae culture.

[0005] To achieve the above-mentioned objectives, the present invention provides a gas-liquid exchange structure, including a gas exchange structure and a liquid exchange structure, the gas exchange structure is located inside the liquid exchange structure, the upper part of the liquid exchange structure is located inside the incubator, the liquid exchange structure is sealed and rotatably connected to the incubator, and the lower part of the liquid exchange structure is located outside the incubator.

[0006] Preferably, the liquid exchange structure includes a sleeve, a plurality of stirring rods are arranged on the top of the sleeve, the internal cavity of the stirring rods is communicated with the internal cavity of the sleeve, the sleeve is sealed and rotatably connected to the incubator, the stirring rod is located inside the incubator, a plurality of connecting holes connecting the internal cavity of the stirring rod with the breeding space inside the incubator are arranged on the stirring rod, a liquid pipe is arranged inside the sleeve, the liquid pipe is sealed and rotatably connected to the sleeve, one end of the liquid pipe is connected to the circulation pump, the other end of the liquid pipe is communicated with the internal cavity of the stirring rod, and a valve for controlling the opening or closing of the liquid pipe is arranged on the liquid pipe.

[0007] Preferably, the gas exchange structure includes an air intake assembly and an exhaust assembly, the air intake assembly includes an inner tube, the inner tube is located inside the stirring rod, a plurality of air outlet pipes are arranged on the inner tube, the air outlet pipes pass through the stirring rod and are connected with the internal breeding space of the incubator, the air outlet pipes are arranged on a fixed ring, the fixed ring is fixedly arranged on a sleeve, the fixed ring and the sleeve are coaxially arranged, an air intake pipe is arranged below the fixed ring, the air intake pipe is located inside the liquid pipe, the top end of the air intake pipe is sealingly and rotatably connected to the fixed ring, the bottom end of the air intake pipe is provided with an air inlet, the air inlet passes through the liquid pipe and is connected to an external air pump.

[0008] Preferably, the exhaust assembly includes an exhaust pipe, which is arranged inside the air inlet pipe. The air inlet end at the top of the exhaust pipe passes through a sleeve and is connected to the breeding space inside the incubator. The exhaust pipe is sealed and rotatably connected to the sleeve. A connecting pipe is provided at the top of the exhaust pipe. The top of the connecting pipe is located above the liquid in the incubator. The bottom end of the exhaust pipe passes through the air inlet pipe and the liquid pipe and is connected to an external gas collecting tank.

[0009] Preferably, the connecting tube is a rigid air tube or a flexible air tube, a float is provided at the top of the flexible air tube, a plurality of air inlet holes are provided on the float, the air inlet holes are connected to the exhaust pipe through the flexible air tube, and the air inlet holes are located above the liquid level of the incubator.

[0010] Preferably, the incubator comprises a cover shell, which is a transparent shell, a bottom plate is arranged at the bottom of the cover shell, a sleeve is sealingly and rotatably connected to the bottom plate, a slot is arranged at the bottom of the sleeve, a card plate is arranged on the bottom plate, the card plate is sealably clamped in the slot, and the cover shell and the bottom plate are clamped together through the card plate and the slot.

[0011] A culture device contains the above-mentioned gas-liquid exchange structure, the interior of the box is provided with a heat-insulating partition, the partition divides the box into an electrical room and a culture room, a plurality of heat-insulating support plates are provided in the culture room, a plurality of vertical plates that divide the support plates into a plurality of placement cavities are provided above the support plates, a mounting seat is slidably provided above the support plates, the culture box is provided on the mounting seat, and a sliding mechanism that drives the mounting seat to slide on the support plate is provided in the electrical room; a heating plate and an irradiation lamp are provided in each placement cavity, a temperature sensor is provided in the placement cavity, the heating plate, the irradiation lamp and the temperature sensor are all electrically connected to a display control device provided on a sealing plate, a carbon dioxide concentration detector and an oxygen concentration detector provided inside the cover are all electrically connected to the display control device, and the sealing plate is fixed on the box to close the electrical room; a heat-insulating box door that closes the culture room is provided on the box, and a handle is provided on the box door.

[0012] Preferably, the mounting seat is provided with a plurality of mounting holes, a rotating sleeve is rotatably arranged in the mounting hole, a socket for inserting the sleeve is arranged at the center of the rotating sleeve, a limiting groove is arranged on the side wall of the socket, a limiting block matched with the limiting groove is arranged on the side wall of the sleeve, the limiting block is inserted in the limiting groove, and the rotating sleeve drives the sleeve to rotate through the limiting groove and the limiting block; a guide rail that guides the sliding of the mounting seat is arranged on the support plate, a guide groove matched with the guide rail is arranged on the lower surface of the mounting seat, the guide rail is located in the guide groove and is slidably connected with the guide groove, a rack is arranged on the guide rail, and a gear meshing with the rack is arranged on the rotating sleeve; a positioning groove for positioning the incubator is arranged on the mounting seat, a positioning platform is arranged on the bottom plate, and the positioning platform is inserted in the positioning groove.

[0013] Preferably, the sliding mechanism includes a motor, a rotating plate is provided on the output shaft of the motor, the rotating plate is hinged to the connecting plate through a transmission plate, a sliding rod corresponding to the mounting seat is provided on the connecting plate, the sliding rod is connected to the mounting seat, and the sliding rod is slidably connected to the partition.

[0014] A culture method, based on the above culture device, comprises the following steps:

[0015] S1. Insert the sleeve of the incubator into the interior of the rotating sleeve, insert the limit block into the limit groove, insert the positioning platform into the positioning groove, connect the liquid pipe to the circulation pump through a flexible water pipe, connect the air inlet to the air pump through a flexible pipe, connect the exhaust pipe to the gas collecting tank through a flexible pipe, the flexible water pipe and the flexible pipe are located between the support plate and the mounting seat, place the mounting seat on the support plate, insert the guide rail into the guide groove, mesh the gear with the rack, and connect one end of the mounting seat to the slide rod;

[0016] S2. The culture liquid is sent into the liquid pipe through the flexible water pipe by a circulation pump, enters the inner cavity of the stirring rod through the liquid pipe, and then enters the incubator through the connecting hole to cultivate microalgae in the incubator; the temperature of the heating plate in the box and the intensity or wavelength of the irradiation lamp are set by the display control device;

[0017] S3. The carbon dioxide concentration and oxygen concentration inside the incubator are detected by a carbon dioxide detector and an oxygen detector. The carbon dioxide enters the air inlet pipe through an air pump and a flexible tube, then enters the inner tube, and is discharged into the incubator through an air outlet pipe. The air outlet pipe is located at the lower part of the incubator and below the liquid surface. The carbon dioxide enters the culture solution to provide the microalgae with reaction components for photosynthesis. The oxygen generated by the photosynthesis of the microalgae overflows from the culture solution and is enriched in the upper part of the culture solution. The oxygen enters the exhaust pipe through a connecting pipe and is collected in a gas collecting tank.

[0018] S4, the motor drives the connecting plate to slide horizontally through the rotating plate and the transmission plate, the connecting plate drives the mounting seat to slide horizontally through the sliding rod, the mounting seat drives the incubator to slide horizontally, the mounting seat drives the rotating sleeve and the gear to slide, the gear drives the rotating sleeve to rotate under the action of the rack, the rotating sleeve drives the sleeve to rotate through the limiting plate and the limiting groove, the sleeve drives the stirring rod to rotate, the mounting seat slides during the sliding process, and the stirring rod stirs the culture solution, so that the carbon dioxide is evenly distributed in the culture solution, which is beneficial to the cultivation of microalgae;

[0019] S5. The circulation pump extracts the culture solution in the incubator through the liquid pipe, the stirring rod and the connecting hole to recover the biomass in the culture solution.

[0020] The advantages and positive effects of the gas-liquid exchange structure, culture device and culture method of the present invention are:

[0021] 1. The sleeve of the liquid exchange structure of the present invention is rotatably connected to the incubator, and a stirring rod is provided on the sleeve. The stirring rod stirs the culture solution, which is beneficial to uniform gas mass transfer and improves the reproduction rate of microalgae; a connecting hole is provided on the stirring rod to realize the exchange of liquid in the incubator, improve the injection of culture solution into the incubator or the recovery of biomass.

[0022] 2. The gas exchange structure of the present invention is arranged inside the liquid exchange structure, and the stirring rod drives the inner tube to rotate synchronously, so that the gas is evenly introduced into the culture liquid, which facilitates the uniform mass transfer of the gas.

[0023] 3. An exhaust pipe is arranged inside the inner tube to extract the oxygen in the incubator through the exhaust pipe. The inner tube is arranged inside the liquid tube. The liquid exchange structure and the gas exchange structure are nested, which is beneficial to reduce the gap requirement of the exchange structure, simplify the structure of the exchange structure, and facilitate the installation of the exchange structure; it is beneficial to reduce the cost of microalgae cultivation and recovery.

[0024] 4. The present invention provides a heating plate and an irradiation lamp in each placement cavity. The heating plate provides temperature for the cultivation of microalgae. Since the support plate has a heat insulation function, the temperature of the placement cavities on different support plates can be set independently and unaffected, which is convenient for studying the effects of different temperatures on microalgae cultivation. The irradiation lamp can be an LED lamp to provide light for the cultivation of microalgae. The vertical plate is used to block light, so that each placement cavity has different light conditions, which is convenient for studying the effects of different environments on microalgae cultivation.

[0025] 5. The present invention arranges a sliding mechanism in the box body to drive the mounting seat to slide. Under the action of the gear and the rack, the rotating sleeve drives the stirring rod to rotate through the sleeve, which simplifies the structure, improves the uniform distribution of carbon dioxide in the culture solution, and is beneficial to gas mass transfer.

[0026] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A cross-sectional view of the installation structure of the gas-liquid exchange structure according to an embodiment of the present invention;

[0028] Figure 2 It is a schematic diagram of a partial cross-section structure of a gas-liquid exchange structure according to an embodiment of the present invention;

[0029] Figure 3 for Figure 2 Middle A: Enlarged view;

[0030] Figure 4 The top structure of the gas-liquid exchange structure of the embodiment of the present invention is shown in FIG. Figure 1 ;

[0031] Figure 5The top structure of the gas-liquid exchange structure of the embodiment of the present invention is shown in FIG. Figure 2 ;

[0032] Figure 6 This is a schematic diagram of the bottom structure of the gas-liquid exchange structure according to an embodiment of the present invention;

[0033] Figure 7 A schematic diagram of the three-dimensional structure of a culture device according to an embodiment of the present invention;

[0034] Figure 8 It is a front view of a culture device according to an embodiment of the present invention;

[0035] Fig. 9 This is a schematic diagram of the internal structure of the culture device box according to an embodiment of the present invention;

[0036] Fig.10 This is a schematic diagram of the structure of a mounting base of a culture device according to an embodiment of the present invention;

[0037] Fig.11 This is a schematic diagram of the structure of the rotating sleeve of the culture device according to an embodiment of the present invention;

[0038] Fig.12 This is a schematic diagram of the three-dimensional structure of the culture device box according to an embodiment of the present invention;

[0039] Fig.13 This is a growth curve of microalgae cultured using the culture device according to an embodiment of the present invention.

[0040] Reference numerals

[0041] 1. Gas-liquid exchange structure; 11. Sleeve; 12. Stirring rod; 13. Fixed ring; 14. Inner tube; 15. Air outlet pipe; 16. Connecting hole; 17. Liquid pipe; 18. Air inlet pipe; 19. Air inlet; 110. Exhaust pipe; 111. First sealed bearing; 112. Valve; 113. Second sealed bearing; 114. Liquid cavity; 115. Air inlet cavity; 116. Exhaust cavity; 117. Limit block; 118. Cover shell; 119. Bottom plate; 120. Card slot; 121. Card plate; 122. Positioning platform; 123. Flexible air pipe; 124. Float;

[0042] 2. Cultivation device; 21. Box body; 22. Partition plate; 23. Support plate; 24. Vertical plate; 25. Heating plate; 26. Irradiation lamp; 27. Mounting seat; 28. Rotating sleeve; 29. ​​Guide rail; 210. Rack; 211. Guide groove; 212. Positioning groove; 213. Mounting hole; 214. Gear; 215. Limiting groove; 216. Motor; 217. Rotating plate; 218. Transmission plate; 219. Connecting plate; 220. Sliding rod; 221. Closing plate; 222. Display control device; 223. Box door; 224. Handle. DETAILED DESCRIPTION

[0043] In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the invented product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In the description of the present invention, it should also be noted that, unless otherwise clearly specified and limited, the terms "setting", "installation", and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0044] The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings.

[0045] like Figure 1-Figure 6 As shown. A gas-liquid exchange structure includes a gas exchange structure and a liquid exchange structure, wherein the gas exchange structure is located inside the liquid exchange structure. The upper portion of the liquid exchange structure is located inside the incubator, and the liquid exchange structure is sealed and rotatably connected to the incubator. The lower portion of the liquid exchange structure is located outside the incubator. The gas exchange structure is used for exchanging reactants and products in the incubator, and for mass transfer of gas during microalgae cultivation. The liquid exchange structure is used for filling or extracting culture liquid into the incubator, which is convenient for recovering biomass during microalgae cultivation.

[0046] The liquid exchange structure includes a sleeve 11, and a plurality of stirring rods 12 are fixedly arranged on the top of the sleeve 11. The stirring rods 12 are radially arranged on the side wall of the sleeve 11, and the internal cavity of the stirring rods 12 is connected to the internal cavity of the sleeve 11. The sleeve 11 is connected to the incubator in a sealed rotation manner through a sealed bearing. The stirring rod 12 is located inside the incubator, and the stirring rod 12 stirs the culture liquid, which is conducive to uniform gas mass transfer. The stirring rod 12 is evenly provided with a plurality of connecting holes 16 connecting the internal cavity of the stirring rod 12 and the cultivation space inside the incubator. A liquid pipe 17 is arranged inside the sleeve 11, and the liquid pipe 17 is connected to the sleeve 11 in a sealed rotation manner through a first sealed bearing 111. One end of the liquid pipe 17 is connected to a circulation pump, and the other end of the liquid pipe 17 is connected to the internal cavity of the stirring rod 12. A valve 112 for controlling the opening or closing of the liquid pipe 17 is arranged on the liquid pipe 17. The culture liquid is sent into the liquid pipe 17 through the circulation pump, and then sent into the culture box through the stirring rod 12 and the connecting hole 16, so as to realize the cultivation of microalgae and the recovery of biomass in the culture box.

[0047] The gas exchange structure includes an air intake assembly and an exhaust assembly. The air intake assembly includes an inner tube 14, which is located inside the stirring rod 12. A plurality of air outlet pipes 15 are evenly fixed on the inner tube 14, and the air outlet pipes 15 pass through the stirring rod 12 and are connected to the internal breeding space of the incubator, so as to deliver the gas in the inner tube 14 into the incubator. The air outlet pipe 15 is fixedly set on the fixing ring 13, and the fixing ring 13 is fixedly set on the sleeve 11. The sleeve 11 drives the inner tube 14 to rotate synchronously with the stirring rod 12 through the fixing ring 13, so as to improve the uniformity of gas entering the incubator. The fixing ring 13 is coaxially arranged with the sleeve 11. An air intake pipe 18 is arranged below the fixing ring 13, and the air intake pipe 18 is located inside the liquid pipe 17 and is coaxially arranged with the liquid pipe 17. The top end of the air intake pipe 18 is connected to the fixing ring 13 in a sealed rotation manner through the second sealing bearing 113. The bottom of the air inlet pipe 18 is provided with an air inlet 19, which passes through the liquid pipe 17 and is connected to an external air pump. The air pump delivers carbon dioxide into the inner tube 14 through the air inlet pipe 18, and then enters the incubator through the air outlet pipe 15 to provide reactants for the photosynthesis of the microalgae.

[0048] The exhaust assembly includes an exhaust pipe 110, and the exhaust pipe 110 is arranged inside the air inlet pipe 18. The air inlet end at the top of the exhaust pipe 110 passes through the sleeve 11 to communicate with the internal breeding space of the incubator. The exhaust pipe 110 is rotatably connected to the sleeve 11 through a sealing bearing. A connecting pipe is provided at the top of the exhaust pipe 110, and the top of the connecting pipe is located above the liquid in the incubator and is located at the top of the incubator as much as possible, which is conducive to extracting the oxygen generated by the photosynthesis of the microalgae out of the incubator. The bottom end of the exhaust pipe 110 passes through the air inlet pipe 18 and the liquid pipe 17 to communicate with the external gas collecting tank. An air pump can also be provided between the gas collecting tank and the exhaust pipe 110, which is more conducive to extracting the oxygen in the incubator.

[0049] The connecting tube can be a rigid air tube, and a plurality of air holes are evenly arranged on the rigid air tube to facilitate oxygen to enter the air tube. The connecting tube can also be a flexible air tube 123, and a float 124 is arranged at the top of the flexible air tube 123. The float 124 is provided with a plurality of air inlet holes, and the air inlet holes are connected to the exhaust pipe 110 through the flexible air tube 123. The float 124 floats above the liquid surface under the buoyancy of the liquid, and the float 124 makes the air inlet hole located above the liquid surface of the incubator, so as to facilitate the extraction of oxygen in the incubator.

[0050] The incubator includes a cover 118, which is a transparent shell, which is conducive to light penetrating the cover 118 and providing light energy for microalgae cultivation. A bottom plate 119 is provided at the bottom of the cover 118, and the sleeve 11 is connected to the bottom plate 119 in a sealed rotational manner through a sealed bearing. A card slot 120 is fixedly provided at the bottom of the sleeve 11, and a card plate 121 is fixedly provided on the bottom plate 119, and the card plate 121 is sealed and carded in the card slot 120. The cover 118 and the bottom plate 119 are connected by the card plate 121 and the card slot 120. A carbon dioxide concentration detector and an oxygen concentration detector are provided in the cover 118, which are used to detect the carbon dioxide concentration and oxygen concentration inside the incubator, so as to facilitate the control of the carbon dioxide and oxygen concentrations in the incubator through the air inlet pipe 18 and the exhaust pipe 110.

[0051] like Figure 7-Figure 9 As shown. A culture device 2 containing a gas-liquid exchange structure 1 includes a box body 21. The box body 21 is a cubic structure. The box body 21 is provided with a heat insulation layer to reduce the impact of the external environment on the internal environment of the box body 21. An insulating partition 22 is provided inside the box body 21. The partition 22 is fixed on the inner wall of the box body 21. The partition 22 divides the box body 21 into an electrical room and a culture room. A plurality of insulating support plates 23 are fixedly provided in the culture room, and a plurality of vertical plates 24 are fixedly provided above the support plate 23 to divide the support plate 23 into a plurality of placement cavities. The vertical plates 24 are fixed on the lower surface of the support plate 23 or the inner surface of the top of the box body 21. A gap is left between the bottom of the vertical plate 24 and the support plate 23 to facilitate the sliding of the mounting seat 27 on the support plate 23.

[0052] Each placement cavity is provided with a heating plate 25 and an irradiation lamp 26. The heating plate 25 is fixed on the inner wall of the box 21, and the irradiation lamp 26 is fixed on the top of the box 21 or the bottom surface of the support plate 23. The heating plate 25 provides temperature for the cultivation of microalgae. Since the support plate 23 has a heat insulation function, the temperature of the placement cavities on different support plates 23 can be set independently and is not affected, which is convenient for studying the effects of different temperatures on microalgae cultivation. The irradiation lamp 26 can use an LED lamp to provide light for the cultivation of microalgae. The vertical plate 24 is used to block the light, so that each placement cavity has different lighting conditions, which is convenient for studying the effects of different environments on microalgae cultivation. A temperature sensor is provided in the placement cavity for monitoring the temperature in the placement cavity and maintaining the temperature in the placement cavity within a set temperature range. The heating plate 25, the irradiation lamp 26, and the temperature sensor are all electrically connected to the display control device 222 provided on the sealing plate 221 using existing technology as needed. The carbon dioxide concentration detector and the oxygen concentration detector provided inside the cover 118 are both electrically connected to the display control device 222.

[0053] like Fig.12As shown. The sealing plate 221 is fixed on the box body 21 to close the electrical room. The box body 21 is provided with an insulated door 223 to close the culture room, and the door 223 is provided with a handle 224. The door 223 is hinged to the box body 21 and locked by an existing lock.

[0054] like Fig.10 , Fig.11 As shown. A mounting seat 27 is slidably provided above the support plate 23, and the incubator is provided on the mounting seat 27. The mounting seat 27 is provided with a plurality of mounting holes 213, and a rotating sleeve 28 is rotatably provided in the mounting hole 213 through a bearing. In this embodiment, three mounting holes 213 are provided on each mounting seat 27. A plug hole for inserting the sleeve 11 is provided at the center of the rotating sleeve 28, and a plurality of limiting grooves 215 are evenly provided on the side wall of the plug hole. A limiting block 117 adapted to the limiting groove 215 is provided on the side wall of the sleeve 11, and the limiting block 117 is inserted into the limiting groove 215. The rotating sleeve 28 drives the sleeve to rotate through the limiting groove 215 and the limiting block 117. A positioning groove 212 for positioning the incubator is provided on the mounting seat 27, and a positioning platform 122 is fixedly provided on the bottom plate 119, and the positioning platform 122 is inserted into the positioning groove 212.

[0055] A guide rail 29 is fixedly provided on the support plate 23 to guide the sliding of the mounting seat 27. A guide groove 211 matching the guide rail 29 is fixedly provided on the lower surface of the mounting seat 27. The guide rail 29 is located in the guide groove 211 and is slidably connected with the guide groove 211. A rack 210 is fixedly provided on the guide rail 29, and a gear 214 meshing with the rack 210 is fixedly provided on the rotating sleeve 28. When the mounting seat 27 slides along the support plate 23, it drives the rotating sleeve 28 to slide synchronously. The gear 214 of the rotating sleeve 28 drives the rotating sleeve 28 to rotate under the action of the rack 210. The rotating sleeve 28 drives the sleeve 11 and the stirring rod 12 to rotate, and the culture liquid in the incubator is stirred.

[0056] The electrical room is provided with a sliding mechanism for driving the mounting seat 27 to slide on the support plate 23. The sliding mechanism includes a motor 216, which is fixedly arranged on the inner wall of the box body 21. A rotating plate 217 is fixedly arranged on the output shaft of the motor 216, and the rotating plate 217 is hinged to the connecting plate 219 through a transmission plate 218. A sliding rod 220 corresponding to the mounting seat 27 is fixedly arranged on the connecting plate 219, and the sliding rod 220 is connected to the mounting seat 27, and the sliding rod 220 is slidably connected to the partition 22. The motor 216 drives the connecting plate 219 to move horizontally through the rotating plate 217 and the transmission plate 218, and the connecting plate 219 drives the mounting plate to slide through the sliding rod 220.

[0057] The culture method based on the above culture device 2 comprises the following steps:

[0058] S1. Insert the sleeve 11 of the incubator into the interior of the rotating sleeve 28, insert the limit block 117 into the limit groove 215, and insert the positioning platform 122 into the positioning groove 212. Connect the liquid pipe 17 to the circulation pump through a flexible water pipe, connect the air inlet 19 to the air pump through a flexible pipe, and connect the exhaust pipe 110 to the gas collecting tank through a flexible pipe. The gas collecting tank, the circulation pump, and the air pump are all arranged outside the box body 21, and the flexible water pipe and the flexible pipe are located between the support plate 23 and the mounting seat 27. Place the mounting seat 27 on the support plate 23, insert the guide rail 29 into the guide groove 211, the gear 214 is meshed with the rack 210, and one end of the mounting seat 27 is connected to the slide bar 220.

[0059] S2. The culture liquid is sent into the liquid cavity 114 of the liquid pipe 17 through the circulation pump through the flexible water pipe, enters the internal cavity of the stirring rod 12 through the liquid pipe 17, and then enters the incubator through the connecting hole 16 to culture the microalgae in the incubator. The temperature of the heating plate 25 in the box 21 and the intensity or wavelength of the irradiation lamp 26 are set through the display control device 222.

[0060] S3. Detect the carbon dioxide concentration and oxygen concentration inside the incubator by using a carbon dioxide detector and an oxygen detector. Carbon dioxide enters the air inlet cavity 115 of the air inlet pipe 18 through an air pump and a flexible tube, then enters the inner tube 14, and is discharged into the incubator through the air outlet pipe 15. The air outlet pipe 15 is located at the lower part of the incubator and below the liquid surface. Carbon dioxide enters the culture solution to provide the microalgae with reaction components for photosynthesis. The oxygen produced by the photosynthesis of the microalgae overflows from the culture solution and is enriched in the upper part of the culture solution. The oxygen enters the exhaust cavity 116 of the exhaust pipe 110 through the connecting pipe and is collected in the gas collecting tank.

[0061] S4, the motor 216 drives the connecting plate 219 to slide horizontally through the rotating plate 217 and the transmission plate 218, the connecting plate 219 drives the mounting seat 27 to slide horizontally through the sliding rod 220, the mounting seat 27 drives the incubator to slide horizontally, the mounting seat 27 drives the rotating sleeve 28 and the gear 214 to slide, the gear 214 drives the rotating sleeve 28 to rotate under the action of the rack 210, the rotating sleeve 28 drives the sleeve 11 to rotate through the limiting plate and the limiting groove 215, and the sleeve 11 drives the stirring rod 12 to rotate. The incubator slides during the sliding process of the mounting seat 27, and the stirring rod 12 stirs the culture solution, so that the carbon dioxide is evenly distributed in the culture solution, which is conducive to the cultivation of microalgae.

[0062] S5. The circulation pump extracts the culture solution in the culture box through the liquid pipe 17, the stirring rod 12 and the connecting hole 16 to recover the biomass in the culture solution.

[0063] The gas-liquid exchange structure and culture device provided by the present invention were successfully applied to the culture of Synechocystis PCC6803 under specific culture conditions. Specifically, the culture temperature was set at 25°C and the light intensity was maintained at 8000 lux. By continuously monitoring the growth of microalgae, the following information was obtained: Fig.13 The results show that as the culture time increases, the dry weight and OD 730 The values ​​all show a significant upward trend. This not only proves that the gas-liquid exchange structure and the culture device of the present invention can effectively promote the growth of microalgae, but also shows that the culture method adopted can provide an ideal growth environment for microalgae. Therefore, the invention has significant application value and promotion potential in the field of microalgae culture technology.

[0064] Therefore, the gas-liquid exchange structure, culture device and culture method described in the present invention can solve the problem of high cost of existing microalgae culture and harvesting, and facilitate the study of the effects of different external environments on microalgae culture.

[0065] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A culture device, characterized in that: Including gas-liquid exchange structure, The gas-liquid exchange structure includes a gas exchange structure and a liquid exchange structure, wherein the gas exchange structure is located inside the liquid exchange structure, the upper portion of the liquid exchange structure is located inside the incubator, the liquid exchange structure is sealed and rotatably connected to the incubator, and the lower portion of the liquid exchange structure is located outside the incubator; The liquid exchange structure comprises a sleeve, a plurality of stirring rods are arranged on the top of the sleeve, the internal cavity of the stirring rods is communicated with the internal cavity of the sleeve, the sleeve is connected to the incubator in a sealed and rotatable manner, the stirring rod is located inside the incubator, a plurality of connecting holes are arranged on the stirring rod that connect the internal cavity of the stirring rod with the culture space inside the incubator, a liquid pipe is arranged inside the sleeve, the liquid pipe is connected to the sleeve in a sealed and rotatable manner, one end of the liquid pipe is connected to the circulation pump, the other end of the liquid pipe is connected to the internal cavity of the stirring rod, and a valve that controls the opening or closing of the liquid pipe is arranged on the liquid pipe; The gas exchange structure comprises an air intake assembly and an exhaust assembly, wherein the air intake assembly comprises an inner tube, the inner tube is located inside the stirring rod, a plurality of air outlet pipes are arranged on the inner tube, the air outlet pipes pass through the stirring rod and are communicated with the culture space inside the incubator, the air outlet pipes are arranged on a fixing ring, the fixing ring is fixedly arranged on the sleeve, the fixing ring and the sleeve are arranged coaxially, an air intake pipe is arranged below the fixing ring, the air intake pipe is located inside the liquid pipe, the top end of the air intake pipe is sealingly and rotatably connected to the fixing ring, the bottom end of the air intake pipe is arranged with an air inlet, the air inlet passes through the liquid pipe and is connected to an external air pump; The exhaust assembly comprises an exhaust pipe, which is arranged inside the air inlet pipe, an air inlet end at the top of the exhaust pipe passes through a sleeve and is connected to the culture space inside the incubator, the exhaust pipe is rotatably connected to the sleeve in a sealed manner, a connecting pipe is arranged at the top of the exhaust pipe, the top of the connecting pipe is located above the liquid in the incubator, and the bottom of the exhaust pipe passes through the air inlet pipe and the liquid pipe and is connected to the external gas collecting tank; An insulating partition is arranged inside the box body, and the partition divides the box body into an electrical room and a culture room. A plurality of insulating support plates are arranged in the culture room, and a plurality of vertical plates are arranged above the support plates to divide the support plates into a plurality of placement cavities. A mounting seat is slidably arranged above the support plate, and the culture box is arranged on the mounting seat. A sliding mechanism for driving the mounting seat to slide on the support plate is arranged in the electrical room; a heating plate and an irradiation lamp are arranged in each placement cavity, and a temperature sensor is arranged in the placement cavity. The heating plate, the irradiation lamp and the temperature sensor are all electrically connected to the display control device arranged on the sealing plate, and the carbon dioxide concentration detector and the oxygen concentration detector arranged inside the cover are all electrically connected to the display control device, and the sealing plate is fixed on the box body to close the electrical room; an insulating box door is arranged on the box body to close the culture room, and a handle is arranged on the box door; The mounting seat is provided with a plurality of mounting holes, a rotating sleeve is rotatably arranged in the mounting hole, a socket for inserting the sleeve is arranged at the center of the rotating sleeve, a limiting groove is arranged on the side wall of the socket, a limiting block matched with the limiting groove is arranged on the side wall of the sleeve, the limiting block is inserted in the limiting groove, and the rotating sleeve drives the sleeve to rotate through the limiting groove and the limiting block; a guide rail having a guiding effect on the sliding of the mounting seat is arranged on the support plate, a guide groove matched with the guide rail is arranged on the lower surface of the mounting seat, the guide rail is located in the guide groove and is slidably connected to the guide groove, a rack is arranged on the guide rail, and a gear meshing with the rack is arranged on the rotating sleeve; a positioning groove for positioning the incubator is arranged on the mounting seat, a positioning platform is arranged on the bottom plate, and the positioning platform is inserted in the positioning groove.

2. A culture device according to claim 1, characterized in that: The connecting pipe is a rigid air pipe or a flexible air pipe. A float is arranged at the top of the flexible air pipe. The float is provided with a plurality of air inlets. The air inlets are connected with the exhaust pipe through the flexible air pipe. The air inlets are located above the liquid level of the incubator.

3. A culture device according to claim 2, characterized in that: The incubator comprises a cover shell which is a transparent shell. A bottom plate is arranged at the bottom of the cover shell. A sleeve is sealingly and rotatably connected with the bottom plate. A card slot is arranged at the bottom of the sleeve. A card plate is arranged on the bottom plate. The card plate is sealably arranged in the card slot. The cover shell and the bottom plate are connected by the card plate and the card slot.

4. A culture device according to claim 3, characterized in that: The sliding mechanism includes a motor, a rotating plate is arranged on the output shaft of the motor, the rotating plate is hinged to the connecting plate through the transmission plate, a sliding rod corresponding to the mounting seat is arranged on the connecting plate, the sliding rod is connected to the mounting seat, and the sliding rod is slidably connected to the partition.

5. A cultivation method, characterized in that: The culture device according to claim 4 comprises the following steps: S1. Insert the sleeve of the incubator into the interior of the rotating sleeve, insert the limit block into the limit groove, insert the positioning platform into the positioning groove, connect the liquid pipe to the circulation pump through a flexible water pipe, connect the air inlet to the air pump through a flexible pipe, connect the exhaust pipe to the gas collecting tank through a flexible pipe, the flexible water pipe and the flexible pipe are located between the support plate and the mounting seat, place the mounting seat on the support plate, insert the guide rail into the guide groove, mesh the gear with the rack, and connect one end of the mounting seat to the slide rod; S2. The culture liquid is sent into the liquid pipe through the flexible water pipe by a circulation pump, enters the inner cavity of the stirring rod through the liquid pipe, and then enters the incubator through the connecting hole to cultivate microalgae in the incubator; the temperature of the heating plate in the box and the intensity or wavelength of the irradiation lamp are set by the display control device; S3. The carbon dioxide concentration and oxygen concentration inside the incubator are detected by a carbon dioxide detector and an oxygen detector. The carbon dioxide enters the air inlet pipe through an air pump and a flexible tube, then enters the inner tube, and is discharged into the incubator through an air outlet pipe. The air outlet pipe is located at the lower part of the incubator and below the liquid surface. The carbon dioxide enters the culture solution to provide the microalgae with reaction components for photosynthesis. The oxygen generated by the photosynthesis of the microalgae overflows from the culture solution and is enriched in the upper part of the culture solution. The oxygen enters the exhaust pipe through a connecting pipe and is collected in a gas collecting tank. S4, the motor drives the connecting plate to slide horizontally through the rotating plate and the transmission plate, the connecting plate drives the mounting seat to slide horizontally through the sliding rod, the mounting seat drives the incubator to slide horizontally, the mounting seat drives the rotating sleeve and the gear to slide, the gear drives the rotating sleeve to rotate under the action of the rack, the rotating sleeve drives the sleeve to rotate through the limiting plate and the limiting groove, the sleeve drives the stirring rod to rotate, the mounting seat slides during the sliding process, and the stirring rod stirs the culture solution, so that the carbon dioxide is evenly distributed in the culture solution, which is beneficial to the cultivation of microalgae; S5. The circulation pump extracts the culture solution in the incubator through the liquid pipe, the stirring rod and the connecting hole to recover the biomass in the culture solution.

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