A colloidal gold immunochromatographic test strip for rapid diagnosis of brucellosis and preparation method thereof

Abstract

The present invention discloses a colloidal gold immunochromatographic test strip for rapid diagnosis of brucellosis and a preparation method thereof, comprising a base plate, a sample pad is installed on the top left side of the base plate, a nitrocellulose membrane is installed on the top middle side of the base plate, a binding pad is inserted between the sample pad and the nitrocellulose membrane, a wicking paper is installed on the top right side of the base plate, a QR code scanning area is provided on one side wall of the base plate, a support block is fixedly installed at the bottom of the base plate, and a non-slip pad is provided at the bottom of the two support blocks. The immune layer test strip of the present invention is more convenient to use and has good stability. During preparation, not only can the soaked binding pad be moved up and down during the soaking process of the binding pad, but also the soaking liquid can be stirred, thereby improving the soaking effect, and at the same time, after the soaking is completed, the collection net frame can be rotated clockwise and counterclockwise by a certain angle, thereby automatically collecting the soaked binding pad, thereby improving work efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of chromatography test strips, and in particular to a colloidal gold immunochromatographic test strip for rapid diagnosis of brucellosis and a preparation method thereof. Background Art

[0002] Brucellosis, also known as undulant fever, Mediterranean remittent fever, Malta fever, etc., is a zoonotic systemic infectious disease caused by Brucella. The main pathogen of brucellosis is Brucella, a Gram-negative bacterium that can be divided into many types.

[0003] When making a rapid diagnosis of brucellosis, colloidal gold immunochromatographic test strips are required for auxiliary detection. When preparing the colloidal gold immunochromatographic test strips, the conjugate pad needs to be soaked in advance. However, during the soaking process of the conjugate pad, it is difficult for the conjugate pad to fully contact with the soaking liquid, which is inconvenient to use. At the same time, after the soaking is completed, the conjugate pad still needs to be collected manually, which is cumbersome to operate and affects work efficiency. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a colloidal gold immunochromatographic test strip for rapid diagnosis of brucellosis and a preparation method thereof.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A colloidal gold immunochromatographic test strip for rapid diagnosis of brucellosis comprises a base plate, a sample pad is installed on the left side of the top of the base plate, a nitrocellulose membrane is installed on the middle side of the top of the base plate, a binding pad is inserted between the sample pad and the nitrocellulose membrane, absorbent paper is installed on the right side of the top of the base plate, a two-dimensional code scanning area is arranged on one side wall of the base plate, a support block is fixedly installed on the bottom of the base plate, and anti-slip pads are arranged on the bottoms of two of the support blocks.

[0007] A method for preparing a colloidal gold immunochromatographic test strip for rapid diagnosis of brucellosis comprises the following steps: preparation of a nitrocellulose membrane, preparation of a sample pad, preparation of a conjugate pad, and assembly of the test strip, wherein the conjugate pad is processed by a preparation device, the preparation device comprises a hollow base, a soaking box is fixedly mounted on the top of the hollow base, a top plate is arranged above the soaking box, a net box is arranged below the top plate, a lifting mechanism is arranged on the hollow base, a stirring mechanism connected to the lifting mechanism is arranged on the hollow base, and a collecting mechanism is arranged on the hollow base. The preparation of the conjugate pad comprises the following steps:

[0008] S1, placing the cut binding pad in a mesh box, mixing the diluent with the preservation solution containing the labeled complex to dilute the labeled complex soaking solution into a certain concentration and pouring it into the soaking box;

[0009] S2. The net box is moved downward by the lifting mechanism, so that the bonding pad in the net box moves downward and is immersed in the soaking liquid. The soaking liquid can be stirred by the stirring mechanism to improve the soaking effect.

[0010] S3, after the soaking is completed, the net box is moved upward by the lifting mechanism, and during this process, the soaked binding pad is collected by the collecting mechanism;

[0011] S4, performing auxiliary drying on the collected binding pad.

[0012] Preferably, the lifting mechanism includes a threaded rod that is penetrated through the top of the hollow base and rotatably connected thereto, the threaded rod is rotatably connected to the top plate, a servo motor is fixedly installed on the inner bottom of the hollow base, the output shaft of the servo motor is fixedly connected to the threaded rod, a guide rod is fixedly connected between the top plate and the hollow base, and two mounting blocks are fixedly installed on the outer side wall of the net box, one of the mounting blocks is threadedly mounted on the threaded rod, and the other mounting block is slidably mounted on the guide rod.

[0013] Preferably, the stirring mechanism includes a rotating shaft that is penetrated through the top of the hollow base and rotatably connected thereto, a stirring rod located in the immersion box is fixedly mounted on the outer wall of the rotating shaft, a cam is fixedly mounted on the outer wall of the threaded rod, two sliding rods are fixedly connected between the inner walls on both sides of the hollow base, the two sliding rods are slidably sleeved with the same sliding plate, the sliding plate is abutted against the cam, a gear is fixedly mounted on the outer wall of the rotating shaft, a rack meshing with the gear is fixedly mounted on the side wall of the sliding plate close to the gear, a first tension spring is fixedly connected between the sliding plate and one side inner wall of the hollow base, and the first tension spring is located on the side of the sliding plate away from the gear.

[0014] Preferably, the collecting mechanism includes a collecting cylinder fixedly mounted on the top of the hollow base, a long rod slidably connected to the top of the collecting cylinder is provided through the top of the collecting cylinder, a second piston slidably connected to the inner wall of the collecting cylinder is fixedly mounted on one end of the long rod located in the collecting cylinder, a connecting rod slidably connected to the long rod and located outside the collecting cylinder is provided through the long rod, an auxiliary block is fixedly mounted on the end of the connecting rod close to the net box, an anti-falling block is fixedly mounted on the end of the connecting rod away from the net box, a connecting pipe connected to the interior of the collecting cylinder is provided on the collecting cylinder, and one end of the connecting pipe connected to the collecting cylinder is located below the second piston.

[0015] Preferably, a collecting net frame is rotatably connected to a side wall of the net box close to the collecting cylinder, a side of the net box close to the collecting cylinder is provided with a resist cylinder fixedly connected to the bottom of a mounting block thereof, a resist rod is penetrated through the bottom of the resist cylinder and is slidably connected to it, a first piston slidably connected to the inner wall of the resist cylinder is fixedly installed at one end of the resist rod located in the resist cylinder, the lower end of the resist rod abuts against the top of the collecting net frame, an end of the connecting tube away from the collecting cylinder is connected to the resist cylinder, and an end of the connecting tube connected to the collecting cylinder is located above the first piston, a round rod is rotatably connected to a side wall of the mounting block fixedly connected to the resist cylinder close to the collecting cylinder, and a reset block located above the auxiliary block is fixedly installed at the bottom of the top plate.

[0016] Preferably, an opening is provided at the top of the collecting net frame, a fixed block is fixedly installed at the bottom of the collecting net frame, a collecting net box located below the collecting net frame is arranged between the two fixed blocks, and plug rods slidably connected to the two fixed blocks are penetrated by the two fixed blocks, and round blocks are fixedly installed at the ends of the two plug rods away from each other, and second tension springs are fixedly connected between the two round blocks and the adjacent fixed blocks, and holes are provided on the two side walls of the collecting net box.

[0017] Preferably, the collecting net frame is matched with the opening, the inserting rod is matched with the inserting hole, the cross sections of the auxiliary block and the reset block are both right-angled trapezoidal shapes, and the cross sections of the connecting rod and the long rod are both rectangular.

[0018] Beneficial effects of the present invention:

[0019] By providing support blocks, anti-slip mats and a QR code scanning area, the stability of the bottom plate can be improved, making it more convenient to use and easy to scan the QR code to obtain relevant information of the test strip.

[0020] By setting up a lifting mechanism, the servo motor can be started to move the placement net box, the collection net frame, the collection net box and the round rod downward as a whole, so that the bonding pad in the placement net box can be immersed in the immersion box. During this process, the round rod will not cause the auxiliary block to move up and down.

[0021] By setting up a lifting mechanism and a stirring mechanism, during the soaking process of the bonding pad, the bonding pad placed in the net box can be moved up and down, and the stirring rod in the soaking box can be rotated clockwise or counterclockwise. Through the mutual cooperation of the two, the bonding pad can be fully contacted with the soaking liquid, thereby improving the soaking effect of the bonding pad.

[0022] By setting up a collecting mechanism, after the soaking of the bonding pad is completed, during the process of placing the net box, collecting net frame, and the overall upward movement of the collecting net box and the round rod, the auxiliary block can be moved up and down once through the round rod, and then the collecting net frame can be rotated clockwise or counterclockwise by a certain angle, thereby realizing automatic collection of the bonding pad in the net box and improving work efficiency.

[0023] The present invention can not only make the soaked conjugate pad move up and down during the soaking process of the conjugate pad, but also stir the soaking liquid to improve the soaking effect, but also make the collection net frame rotate clockwise or counterclockwise by a certain angle after the soaking is completed, so as to automatically collect the soaked conjugate pad, thereby improving work efficiency, being more convenient to use, and making the immune layer test strip more convenient to use, and increasing the stability of the bottom plate placement. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the three-dimensional structure of a colloidal gold immunochromatographic test strip for rapid diagnosis of brucellosis proposed by the present invention;

[0025] Figure 2 It is a schematic diagram of the three-dimensional structure of the bonding pad preparation device proposed by the present invention;

[0026] Figure 3 For the present invention Figure 2 A schematic diagram of the enlarged structure at A in the middle;

[0027] Figure 4 It is a schematic diagram of the three-dimensional structure inside the soaking box of the present invention;

[0028] Figure 5 It is a schematic diagram of the three-dimensional structure inside the cylinder of the present invention;

[0029] Figure 6 It is a schematic diagram of the three-dimensional structure inside the collecting tube of the present invention;

[0030] Figure 7 It is a schematic diagram of the three-dimensional structure of the opening of the present invention.

[0031] In the figure: 1 bottom plate, 2 nitrocellulose membrane, 3 absorbent paper, 4 binding pad, 5 sample pad, 6 QR code scanning area, 7 support block, 8 anti-skid pad, 9 hollow base, 10 threaded rod, 11 top plate, 12 mounting block, 13 opening, 14 placing net box, 15 push tube, 16 connecting pipe, 17 push rod, 18 collecting net frame, 19 jack, 20 guide rod, 21 collecting tube, 22 soaking box, 23 sliding rod, 24 rotating shaft, 25 gear, 26 rack, 27 servo motor, 28 first tension spring, 29 cam, 30 sliding plate, 31 collecting net box, 32 round rod, 33 reset block, 34 stirring rod, 35 first piston, 36 auxiliary block, 37 first spring, 38 connecting rod, 39 anti-slip block, 40 long rod, 41 second spring, 42 second piston, 43 round block, 44 second tension spring, 45 plug rod, 46 fixed block. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0033] Reference Figure 1 A colloidal gold immunochromatographic test strip for rapid diagnosis of brucellosis includes a bottom plate 1, a sample pad 5 is installed on the top left side of the bottom plate 1, a nitrocellulose membrane 2 is installed on the top middle side of the bottom plate 1, a binding pad 4 is inserted between the sample pad 5 and the nitrocellulose membrane 2, an absorbent paper 3 is installed on the top right side of the bottom plate 1, a two-dimensional code scanning area 6 is arranged on one side wall of the bottom plate 1, a support block 7 is fixedly installed on the bottom of the bottom plate 1, and anti-slip pads 8 are arranged at the bottom of the two support blocks 7.

[0034] Reference Figure 2-Figure 7 , a method for preparing a colloidal gold immunochromatographic test strip for rapid diagnosis of brucellosis, comprising the following steps: preparation of a nitrocellulose membrane 2, preparation of a sample pad 5, preparation of a binding pad 4, and assembly of the test strip, wherein the binding pad 4 is processed by a preparation device, the preparation device comprising a hollow base 9, a soaking box 22 is fixedly installed on the top of the hollow base 9, a top plate 11 is arranged above the soaking box 22, a net box 14 is arranged below the top plate 11, a lifting mechanism is arranged on the hollow base 9, the lifting mechanism comprises a threaded rod 10 which is arranged through the top of the hollow base 9 and is rotatably connected thereto, the threaded rod 10 is rotatably connected to the top plate 11, a servo motor 27 is fixedly installed on the inner bottom of the hollow base 9, the output shaft of the servo motor 27 is fixedly connected to the threaded rod 10, a guide rod 20 is fixedly connected between the top plate 11 and the hollow base 9, and two mounting blocks 12 are fixedly installed on the outer wall of the net box 14, one mounting block 12 is threadedly sleeved on the threaded rod 10, and the other mounting block 12 is slidably sleeved on the guide rod 20;

[0035] A stirring mechanism connected to the lifting mechanism is provided on the hollow base 9, and the stirring mechanism includes a rotating shaft 24 which is arranged on the top of the hollow base 9 and is rotatably connected thereto, a stirring rod 34 located in the immersion box 22 is fixedly installed on the outer wall of the rotating shaft 24, a cam 29 is fixedly installed on the outer wall of the threaded rod 10, two sliding rods 23 are fixedly connected between the inner walls on both sides of the hollow base 9, the two sliding rods 23 are slidably sleeved with the same sliding plate 30, the sliding plate 30 is against the cam 29, a gear 25 is fixedly installed on the outer wall of the rotating shaft 24, a rack 26 meshing with the gear 25 is fixedly installed on the side wall of the sliding plate 30 close to the gear 25, a first tension spring 28 is fixedly connected between the sliding plate 30 and the inner wall of one side of the hollow base 9, and the first tension spring 28 is located on the side of the sliding plate 30 away from the gear 25;

[0036] The hollow base 9 is provided with a collecting mechanism, which includes a collecting cylinder 21 fixedly mounted on the top of the hollow base 9, a long rod 40 slidably connected to the collecting cylinder 21 is provided through the top of the collecting cylinder 21, a second piston 42 slidably connected to the inner wall of the collecting cylinder 21 is fixedly mounted on one end of the long rod 40 located in the collecting cylinder 21, a connecting rod 38 slidably connected to the collecting cylinder 21 and located outside the collecting cylinder 21 is provided through the long rod 40, an auxiliary block 36 is fixedly mounted on one end of the connecting rod 38 close to the net box 14, and an anti-dropping block is fixedly mounted on the end of the connecting rod 38 away from the net box 14 Block 39, a connecting pipe 16 connected to the interior of the collecting cylinder 21 is provided on the collecting cylinder 21, and one end of the connecting pipe 16 connected to the collecting cylinder 21 is located below the second piston 42, and a collecting net frame 18 is rotatably connected to a side wall of the placing net box 14 close to the collecting cylinder 21, and a side of the placing net box 14 close to the collecting cylinder 21 is provided with a resisting cylinder 15 fixedly connected to the bottom of one of the mounting blocks 12, and a resisting rod 17 slidably connected to the bottom of the resisting cylinder 15 is penetrated and provided, and a first piston 35 slidably connected to the inner wall of the resisting cylinder 15 is fixedly installed on one end of the resisting rod 17 located in the resisting cylinder 15, The lower end of the push rod 17 is against the top of the collecting net frame 18, the end of the connecting pipe 16 away from the collecting cylinder 21 is connected to the push cylinder 15, and the end of the connecting pipe 16 connected to the collecting cylinder 21 is located above the first piston 35. The mounting block 12 fixedly connected to the push cylinder 15 is rotatably connected to a round rod 32 on a side wall close to the collecting cylinder 21, and a reset block 33 located above the auxiliary block 36 is fixedly installed at the bottom of the top plate 11. An opening 13 is opened at the top of the collecting net frame 18, and a fixed block 46 is fixedly installed at the bottom of the collecting net frame 18. A The collecting net box 31 is located below the collecting net frame 18, and the two fixing blocks 46 are penetrated by the plug rods 45 which are slidably connected thereto, and the ends of the two plug rods 45 which are away from each other are fixedly installed with round blocks 43, and the two round blocks 43 are fixedly connected to the adjacent fixing blocks 46 by the second tension spring 44, and the two side walls of the collecting net box 31 are provided with plug holes 19, the collecting net frame 18 is adapted to the opening 13, and the plug rods 45 are adapted to the plug holes 19, the cross sections of the auxiliary block 36 and the reset block 33 are both right-angled trapezoidal, and the cross sections of the connecting rod 38 and the long rod 40 are both rectangular;

[0037] The preparation of the conjugate pad 4 comprises the following steps:

[0038] S1, placing the cut binding pad 4 in the placement net box 14, mixing the diluent with the preservation solution containing the labeled complex to dilute the soaking solution of the labeled complex to a certain concentration and pouring it into the soaking box 22;

[0039] S2, by means of the lifting mechanism, the net box 14 is moved downward, so that the bonding pad 4 in the net box 14 is moved downward to be immersed in the soaking liquid, and the soaking liquid can be stirred by the stirring mechanism to improve the soaking effect;

[0040] S3, after the soaking is completed, the net box 14 is moved upward by the lifting mechanism, and during this process, the soaked binding pad 4 is collected by the collecting mechanism;

[0041] S4, performing auxiliary drying on the collected binding pad 4.

[0042] When the present invention is used, in the initial state, the second piston 42 abuts against the inner bottom of the collecting cylinder 21, and the abutting rod 17 abuts against the top of the collecting net frame 18 so that the collecting net frame 18 is close to the bottom of the placing net box 14. The inserting rod 45 cooperates with the inserting hole 19 to fix the collecting net box 31 below the collecting net frame 18, and the cut binding pad 4 is placed in the placing net box 14. The diluent and the preservation liquid containing the marking complex are mixed and diluted into a soaking liquid of a certain concentration of the marking complex and poured into the soaking box 22. Then, the servo motor 27 is started to make the threaded rod 10 rotate clockwise. By setting the guide rod 20, the two mounting blocks 12, the round rod 32, the placing net box 14, and the collecting net can be rotated clockwise. The frame 18 and the collecting net box 31 move downward as a whole until the binding pad 4 in the placing net box 14 is immersed in the immersion liquid. During this process, the round rod 32 will contact the upper inclined surface of the auxiliary block 36. However, when the round rod 32 continues to move downward, the second piston 42 abuts against the inner bottom of the collecting cylinder 21, and the long rod 40 and the auxiliary block 36 cannot move downward, so that the auxiliary block 36 moves toward the direction close to the long rod 40, and the first spring 37 is compressed. Finally, the round rod 32 slides along the inclined surface of the auxiliary block 36 until it moves to the bottom of the auxiliary block 36. After the round rod 32 moves to the bottom of the auxiliary block 36, the elastic force generated by the compression of the first spring 37 can make the auxiliary block 36 return to the initial position.

[0043] During the soaking process of the bonding pad 4, the servo motor 27 can be used to make the threaded rod 10 reciprocate clockwise and counterclockwise to rotate a certain angle, so that the bonding pad 4 placed in the net box 14 can move up and down in the soaking box 22 (the round rod 32 is always located below the auxiliary block 36 during this process). When the threaded rod 10 rotates, the cam 29 is also driven to rotate. By setting the sliding plate 30 and the first tension spring 28, the rack 26 can move back and forth. The rack 26 is meshed with the gear 25 to make the rotating shaft 24 rotate clockwise and counterclockwise. By setting the stirring rod 34, the soaking liquid in the soaking box 22 can be stirred. By the up and down movement of the bonding pad 4, the soaking effect can be improved.

[0044] After the soaking is completed, the two mounting blocks 12, the round rod 32, the placing net box 14, the collecting net frame 18 and the collecting net box 31 can be moved upward as a whole through the servo motor 27. During the upward movement of the round rod 32, the round rod 32 will contact with the bottom of the auxiliary block 36, and as the round rod 32 continues to move upward, the auxiliary block 36, the long rod 40 and the second piston 42 can move upward as a whole, the second spring 41 is compressed, and during the movement of the auxiliary block 36, the inclined surface on the auxiliary block 36 will contact with the inclined surface of the reset block 33, and as the round rod 32 continues to move upward, the auxiliary block 36 can move in the direction close to the long rod 40, the first spring 37 is compressed again, and finally the round rod 32 moves to the top of the auxiliary block 36 and returns to the initial position. When the round rod 32 moves to the top of the auxiliary block 36, the elastic force generated by the compression of the first spring 37 can make the auxiliary block 36 return to the initial position, and the elastic force generated by the compression of the second spring 41 can make the second piston 42 move downward and return to the initial position;

[0045] During the upward movement of the second piston 42, the incompressible fluid in the cylinder 15 can be sucked into the collecting cylinder 21 through the connecting pipe 16, so that the first piston 35 and the rod 17 move upward. Since the rotating connection between the collecting net frame 18 and the placing net box 14 is close to the cylinder 15 and the gravity of the collecting net box 31, the collecting net frame 18 can rotate counterclockwise (see FIG. Figure 2 The second spring 41 is compressed and the elastic force generated by the second piston 42 causes the incompressible fluid in the collecting cylinder 21 to be squeezed into the push tube 15 through the connecting pipe 16, so that the first piston 35 and the push rod 17 move downward, and the push rod 17 presses one end of the collecting frame 18, so that the collecting frame 18 can be rotated clockwise until it is horizontal, and the second tension spring 44 can be pulled out by pulling the round block 43 outward, and the insertion rod 45 is away from the insertion hole 19, so that the placing network box 14 can be removed, and the binding pad 4 in the collecting network box 31 can be easily transferred for drying.

[0046] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A preparation device for a colloidal gold immunochromatographic test strip conjugate pad, characterized in that: The preparation device comprises a hollow base (9), a soaking box (22) is fixedly mounted on the top of the hollow base (9), a top plate (11) is arranged above the soaking box (22), a net box (14) is arranged below the top plate (11), a lifting mechanism is arranged on the hollow base (9), a stirring mechanism connected to the lifting mechanism is arranged on the hollow base (9), the lifting mechanism comprises a threaded rod (10) penetrating the top of the hollow base (9) and rotatably connected thereto, the threaded rod (10) ) is rotatably connected to the top plate (11), a servo motor (27) is fixedly mounted on the inner bottom of the hollow base (9), an output shaft of the servo motor (27) is fixedly connected to the threaded rod (10), a guide rod (20) is fixedly connected between the top plate (11) and the hollow base (9), two mounting blocks (12) are fixedly mounted on the outer side wall of the net box (14), one of the mounting blocks (12) is threadedly mounted on the threaded rod (10), and the other mounting block (12) is slidably mounted on the guide rod (20); The hollow base (9) is provided with a collecting mechanism, the collecting mechanism comprising a collecting cylinder (21) fixedly mounted on the top of the hollow base (9), a long rod (40) slidably connected to the collecting cylinder (21) being provided through the top of the collecting cylinder (21), a second piston (42) slidably connected to the inner wall of the collecting cylinder (21) being fixedly mounted on one end of the long rod (40) located inside the collecting cylinder (21), a connecting rod (38) slidably connected to the long rod (40) and located outside the collecting cylinder (21) being provided through the long rod (40), an auxiliary block (36) being fixedly mounted on one end of the connecting rod (38) close to the placement net box (14), an anti-dropping block (39) being fixedly mounted on one end of the connecting rod (38) away from the placement net box (14), a connecting pipe (16) being connected to the interior of the collecting cylinder (21), one end of the connecting pipe (16) being connected to the collecting cylinder (21) being located below the second piston (42), the placement net box (14) being close to the collection cylinder (21) ) is rotatably connected to a side wall of the collecting cylinder (21); a side of the placing net box (14) close to the collecting cylinder (21) is provided with a resisting cylinder (15) fixedly connected to the bottom of a mounting block (12); a resisting rod (17) is provided through the bottom of the resisting cylinder (15) and is slidably connected to the resisting cylinder (15); a first piston (35) slidably connected to the inner wall of the resisting cylinder (15) is fixedly installed at one end of the resisting rod (17) located in the resisting cylinder (15); and the lower end of the resisting rod (17) is slidably connected to the collecting cylinder (21). The top of the screen frame (18) is abutted against the top of the screen frame (18), one end of the connecting pipe (16) away from the collecting cylinder (21) is connected to the anti-cylinder (15), and the end of the connecting pipe (16) connected to the anti-cylinder (15) is located above the first piston (35), and a round rod (32) is rotatably connected to a side wall of the mounting block (12) close to the collecting cylinder (21), and a reset block (33) located above the auxiliary block (36) is fixedly installed at the bottom of the top plate (11).

2. The device for preparing a colloidal gold immunochromatographic test strip conjugate pad according to claim 1, characterized in that: The top of the collecting net frame (18) is provided with an opening (13), the bottom of the collecting net frame (18) is fixedly provided with a fixing block (46), a collecting net box (31) located below the collecting net frame (18) is provided between the two fixing blocks (46), a plug rod (45) slidably connected to the two fixing blocks (46) is provided through the two fixing blocks (46), a round block (43) is fixedly provided at one end of the two plug rods (45) away from each other, a second tension spring (44) is fixedly connected between the two round blocks (43) and the adjacent fixing blocks (46), and insertion holes (19) are provided on the two side walls of the collecting net box (31), the collecting net frame (18) is adapted to fit the opening (13), and the plug rod (45) is adapted to fit the insertion hole (19).

3. The device for preparing a colloidal gold immunochromatographic test strip conjugate pad according to claim 2, characterized in that: The cross-sections of the auxiliary block (36) and the reset block (33) are both in the shape of a right-angled trapezoid, and the cross-sections of the connecting rod (38) and the long rod (40) are both in the shape of a rectangle.

4. The device for preparing a colloidal gold immunochromatographic test strip conjugate pad according to claim 3, characterized in that: The stirring mechanism comprises a rotating shaft (24) which is arranged through the top of the hollow base (9) and is rotatably connected thereto; a stirring rod (34) located in the soaking box (22) is fixedly mounted on the outer wall of the rotating shaft (24); a cam (29) is fixedly mounted on the outer wall of the threaded rod (10); two sliding rods (23) are fixedly connected between the inner walls on both sides of the hollow base (9); a same sliding plate (30) is slidably mounted on the two sliding rods (23); the sliding plate (30) abuts against the cam (29); a gear (25) is fixedly mounted on the outer wall of the rotating shaft (24); a rack (26) meshingly connected to the gear (25) is fixedly mounted on a side wall of the sliding plate (30) close to the gear (25); a first tension spring (28) is fixedly connected between the sliding plate (30) and the inner wall on one side of the hollow base (9); the first tension spring (28) is located on a side of the sliding plate (30) away from the gear (25).

Images