A multi-dimensional anti-pollution composition containing oxyresveratrol suitable for sensitive skin and its application

Abstract

This application relates to the technical field of cosmetics, and specifically discloses a multi-dimensional anti-pollution composition containing resveratrol oxide for sensitive skin and its application. The multi-dimensional anti-pollution composition includes resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and Annona muricata extract. The mass ratio of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and Annona muricata extract is (0.1 - 3):(0.001 - 0.1):(1 - 10):(0.1 - 5). The anti-pollution composition provided by this application can achieve the effects of soothing, repairing, and whitening by simultaneously resisting various pollutants such as air pollution, ultraviolet rays, and heavy metals, and comprehensively solve the problems of skin sallowness and sensitivity.

Description

Technical Field

[0001] The present application relates to the technical field of cosmetics, and particularly to a multi-dimensional anti-pollution composition containing resveratrol oxide suitable for sensitive skin and its application. Background Art

[0002] The impact of environmental pollution on human skin has attracted increasing attention. For example, air pollution, ultraviolet radiation, chemical substances, etc. may all have negative effects on skin health. Fine particulate matter in the air (such as PM2.5) can penetrate the skin barrier and enter the deep layer of the skin, leading to inflammation, allergies, and accelerated skin aging. Ozone in the air can oxidize skin lipids, causing skin dullness, dryness, and a decrease in elasticity. At the same time, it may also irritate the skin, resulting in redness, swelling, and sensitivity. UVA radiation can penetrate the dermis layer and damage collagen and elastic fibers, leading to skin relaxation and wrinkle formation; UVB mainly affects the epidermis layer, which may cause sunburn, pigmentation, and an increased risk of skin cancer. Heavy metal pollutants such as lead and mercury can be absorbed through the skin, affecting skin health and even causing poisoning reactions. Certain chemical substances (such as certain components in cosmetics) can damage the skin barrier, resulting in skin dryness, allergies, and irritation.

[0003] Currently, many skin care products can only focus on a single function, such as antioxidant, moisturizing, or anti-inflammatory, lacking comprehensive solutions for anti-pollution, removing yellowish complexion, and improving sensitivity. There are few products that can effectively remove skin yellowish complexion and repair the skin at the same time. Existing research often focuses on whitening while ignoring the root causes of the yellowish complexion problem. Moreover, the anti-pollution components in existing research form a temporary protective layer on the skin surface, but cannot effectively isolate pollutants or repair skin damage for a long time. Summary of the Invention

[0004] The purpose of the present application is to overcome the deficiencies of the above-mentioned existing technologies and provide a multi-dimensional anti-pollution composition containing resveratrol oxide suitable for sensitive skin and its application. The anti-pollution composition provided by the present application can achieve the effects of soothing, repairing, and whitening by simultaneously resisting various pollutants such as air pollution, ultraviolet rays, and heavy metals, and comprehensively solve the problems of skin yellowish complexion and sensitivity.

[0005] To achieve the above purpose, the technical solution adopted by the present application is as follows:

[0006] The present application provides a multi-dimensional anti-pollution composition, which includes resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and Annona muricata extract;

[0007] The mass ratio of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and Annona muricata extract is (0.1 - 3):(0.001 - 0.1):(1 - 10):(0.1 - 5).

[0008] The inventors of the present application have found through research that the compounding of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract in specific ratios can resist multiple pollutants such as air pollution, ultraviolet rays, and heavy metals simultaneously, achieving the effects of soothing, repairing, and whitening. Among them, the combination of resveratrol oxide and epigallocatechin gallate glucoside can not only neutralize free radicals caused by pollution through antioxidant effects, reduce the oxidative damage of the skin caused by environmental pollution, protect the skin from UV radiation damage, but also jointly resist air pollution by enhancing the skin barrier, effectively reducing the oxidative stress of the skin, preventing damage caused by ultraviolet rays, and soothing skin inflammation, improving skin sensitivity and redness.

[0009] Meanwhile, the addition of chitosan oligosaccharide can promote skin moisturization, repair damaged skin barriers, help reduce skin inflammatory responses, improve skin smoothness and elasticity, and at the same time promote uniform pigmentation, reduce skin sallowness, making it more capable of coping with environmental stress.

[0010] Adding annona muricata extract to the multi-dimensional anti-pollution composition of the present application can protect the skin from environmental pollution and ultraviolet rays, significantly reduce skin redness and sensitivity caused by pollution, and it also has antibacterial properties, helping to keep the skin clean and healthy. The combined effect of the above substances can make the skin more comfortable and healthy when facing environmental pollution.

[0011] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the mass ratio of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract is (0.2 - 2):(0.005 - 0.05):(4 - 8):(0.5 - 3).

[0012] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the mass ratio of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract is (0.5 - 1):(0.01 - 0.02):(6 - 7):(1 - 2).

[0013] The present application has found that when resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract are in the above mass ratio, it is beneficial to effectively isolate pollutants or repair skin damage for a long time, while achieving the effects of soothing, repairing, and whitening, and comprehensively solving the problems of skin sallowness and sensitivity.

[0014] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the multi-dimensional anti-pollution composition further includes semen vaccariae extract, adansonia digitata pulp extract, and terminalia chebula fruit extract.

[0015] The inventors of the present application have found through research that by adding specific amounts of semen vaccariae extract, baobab pulp extract, and chebula fruit extract to the present application and compounding them with resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract, it can further promote the multi-dimensional anti-pollution composition to resist various pollutants such as air pollution, ultraviolet rays, and heavy metals, and comprehensively enhance the effects of the multi-dimensional anti-pollution composition in terms of soothing, repairing, and whitening.

[0016] Adding semen vaccariae extract to the multi-dimensional anti-pollution composition can improve the soothing and anti-inflammatory effects of the multi-dimensional anti-pollution composition, effectively reduce skin redness and allergic reactions, and promote blood circulation in the skin, which helps to improve the skin's self-repair ability and overall health; adding baobab pulp extract to the multi-dimensional anti-pollution composition can enhance the strong antioxidant and moisturizing effects of the multi-dimensional anti-pollution composition, help protect the skin from pollution and ultraviolet damage, and at the same time improve the elasticity and luster of the skin; adding chebula fruit extract to the multi-dimensional anti-pollution composition can reduce skin inflammation, improve skin health, and the prepared multi-dimensional anti-pollutant helps to improve the firmness and smoothness of the skin and reduce skin yellowness.

[0017] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the mass ratio of the annona muricata extract, semen vaccariae extract, baobab pulp extract, and chebula fruit extract is (1 - 2):(0.1 - 3):(0.1 - 3):(0.01 - 1).

[0018] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the mass ratio of the annona muricata extract, semen vaccariae extract, baobab pulp extract, and chebula fruit extract is (1 - 2):(0.5 - 2):(0.5 - 2):(0.05 - 0.5).

[0019] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the mass ratio of the annona muricata extract, semen vaccariae extract, baobab pulp extract, and chebula fruit extract is (1 - 2):(1 - 1.5):(1 - 1.5):(0.1 - 0.2).

[0020] When the annona muricata extract, semen vaccariae extract, baobab pulp extract, and chebula fruit extract are combined in the above specific mass ratio, the multi-dimensional anti-pollution composition achieves better multiple functions such as soothing, repairing, and improving skin yellowness. When the semen vaccariae extract and baobab pulp extract are in excess, it will affect the synergistic effect of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract.

[0021] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the preparation method of the annona muricata extract comprises the following steps:

[0022] Take the fruits of annona muricata, crush and sieve them, then add them to an ethanol solution for ultrasonic extraction. After the ultrasonic extraction is completed, centrifuge to collect the supernatant and concentrate it to obtain the annona muricata extract.

[0023] The present application has found through research that the annona muricata extract is rich in flavonoid components and has excellent activities such as anti-inflammatory, antibacterial, and antioxidant. Especially when prepared and extracted by the method provided in the present application, the obtained annona muricata extract can better cooperate with other components to achieve more excellent comprehensive effects.

[0024] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, in the ultrasonic extraction, the temperature is 50 - 65°C, the time is 20 - 40 min, and the power is 100 - 150 W.

[0025] The inventors of the present application have conducted a large number of experimental studies on the temperature and power of ultrasonic extraction and found that using the temperature and power of ultrasonic extraction within the above ranges of the present application is beneficial to improving the extraction efficiency of the annona muricata extract and can more efficiently prepare a higher-purity annona muricata extract in a shorter time.

[0026] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, in the ethanol solution, the mass percentage of ethanol is 55 - 65%;

[0027] In the ultrasonic extraction, the solid-liquid ratio is 1 g : (10 - 20) mL.

[0028] The present application can further increase the content of flavonoids in the annona muricata extract by using the ethanol solution within the above mass concentration range and the above solid-liquid ratio, which is beneficial to the application of the annona muricata extract in the multi-dimensional anti-pollution composition.

[0029] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, the mesh number of the sieving is 60 - 80 meshes.

[0030] As a preferred embodiment of the multi-dimensional anti-pollution composition described in the present application, before crushing the fruits of annona muricata, it further includes a step of drying to constant weight at 45 - 55°C.

[0031] The present application also provides the application of the above multi-dimensional anti-pollution composition in the preparation of cosmetics.

[0032] The present application also provides a cosmetic, comprising the above multi-dimensional anti-pollution composition; the mass percentage of the multi-dimensional anti-pollution composition in the cosmetic is 1 - 20%.

[0033] The inventors of the present application have found through research that cosmetics prepared with the multi-dimensional anti-pollution composition, while controlling the multi-dimensional anti-pollution composition within the above mass percentage range, can enable it to have a good effect of resisting various pollutants such as air pollution, ultraviolet rays, and heavy metals, and at the same time achieve a comprehensive effect of soothing, repairing, and whitening.

[0034] Preferably, the cosmetics include at least one of lotion, emulsion, cream, facial mask, essence, and spray.

[0035] As a preferred embodiment of the cosmetics of the present application, the cosmetics are emulsion; the emulsion also includes the following components in mass percentage: thickener 0.05 - 0.3%, humectant 0.5 - 1%, pH regulator 0.01 - 0.3%, emulsifier 0.5 - 5% and emulsifier 0.5 - 3%.

[0036] As a preferred embodiment of the cosmetics of the present application, the thickener includes at least one of xanthan gum, carbomer, acryloyldimethyltaurate / VP copolymer, ammonium acryloyldimethyltaurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0037] The humectant includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerol, pullulan, and ceramide;

[0038] The pH regulator includes at least one of arginine, tromethamine, and disodium EDTA;

[0039] The emulsifier includes at least one of glyceryl caprylate / caprate, C14-22 alcohol, C12-20 alkyl glucoside, cetearyl glucoside, isononyl isononanoate, pentaerythrityl tetraester, polydimethylsiloxane, stearyl alcohol, hydroxystearic acid, polymethylsilsesquioxane, pentaerythrityl distearate, and sucrose stearate;

[0040] The emulsifier includes at least one of 1,2-hexanediol, p-hydroxyacetophenone, and acrylate / C10-30 alkyl acrylate cross-linked polymer.

[0041] Compared with the prior art, the present application has the following beneficial effects:

[0042] (1) The multi-dimensional anti-pollution composition provided by this application has comprehensive multiple functions. The technical solution of this application combines resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract to achieve multiple functions such as anti-pollution, antioxidant, soothing, repair, and improvement of skin sallowness. Moreover, semen vaccariae extract, baobab pulp extract, and terminalia chebula fruit extract are added to the composition to enhance the multiple functions. Different from the skin care products in the prior art that often can only address a single problem, this application provides a comprehensive solution that can more comprehensively meet the needs of the skin when facing environmental pollution and sensitivity problems.

[0043] (2) The multi-dimensional anti-pollution composition provided by this application is mild and suitable for sensitive skin. The plant extracts selected in this application (such as annona muricata extract, semen vaccariae extract) and chitosan oligosaccharide have good mildness and low irritation, which makes the cosmetics of this application more suitable for sensitive skin. In the prior art, many anti-pollution skin care products may cause irritation to sensitive skin, while this solution reduces the risk of irritation and improves the safety of the product by optimizing the selection and ratio of ingredients.

[0044] (3) The multi-dimensional anti-pollution composition provided by this application has long-lasting and effective anti-pollution and repair capabilities. This solution uses powerful antioxidants such as resveratrol oxide and epigallocatechin gallate glucoside, as well as other anti-inflammatory and repair ingredients such as baobab pulp extract and terminalia chebula fruit extract to provide long-lasting anti-pollution effects and repair capabilities. Many products in the prior art may only provide protection for a short period of time, while the technical solution of this application aims to achieve long-term and effective protection to improve skin health and appearance. Description of the Drawings

[0045] Figure 1 It is a comparison chart of before use and after 7 days of use for the blank application example and application example 15 in Effect Example 3. Detailed Embodiments

[0046] To better illustrate the purpose, technical solution, and advantages of this application, the following will further illustrate this application in combination with specific embodiments.

[0047] In this application, among the technical features described in an open-ended manner, it includes a closed technical solution composed of the listed features, as well as an open technical solution containing the listed features.

[0048] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are considered continuous and include the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when the range refers to integers, it includes each integer between the minimum and maximum values of the range. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all sub-ranges subsumed therein.

[0049] In the following examples and comparative examples, unless otherwise specified, the experimental methods used are conventional methods, and the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels, and the component raw materials used in each parallel experiment are of the same kind.

[0050] The resveratrol oxide and chitosan oligosaccharide used in this application are both conventional commercially available products;

[0051] Epigallocatechin gallate glucoside: EGCG, purchased from Givaudan;

[0052] Vaccaria segetalis extract: product number L2-SCB2519, purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.;

[0053] Adansonia digitata pulp extract: product number realinpioneer3, purchased from Shaanxi Ruilin Panier Biotechnology Co., Ltd.;

[0054] Terminalia chebula fruit extract: product number SXHLHZTQW, purchased from Shaanxi Hengling Natural Biological Products Co., Ltd.

[0055] Annona squamosa seed extract: product number TK-FLZZ-001, Xi'an Tiankang Biotechnology Co., Ltd.

[0056] This application provides Annona squamosa extracts 1-4.

[0057] Among them, the preparation method of Annona squamosa extract 1 includes the following steps:

[0058] Take the fruits of Annona squamosa, dry them to constant weight at 50 °C, then pulverize and sieve them, add them to an ethanol solution (the mass percentage of ethanol is 60%), carry out ultrasonic extraction, the temperature of ultrasonic extraction is 55 °C, the time is 30 min, the power is 120 W, the solid-liquid ratio is 1 g:15 mL. After the ultrasonic extraction is completed, centrifuge to collect the supernatant and concentrate it to obtain Annona squamosa extract 1.

[0059] The preparation method of Annona squamosa extract 2 includes the following steps:

[0060] The fruits of Annona cherimola Mill. were dried to a constant weight at 50 °C, then crushed and sieved, and added to an ethanol solution (the mass percentage of ethanol was 55%) for ultrasonic extraction. The temperature of ultrasonic extraction was 50 °C, the time was 20 min, the power was 100 W, and the solid-liquid ratio was 1 g: 10 mL. After ultrasonic extraction, the supernatant was collected by centrifugation and concentrated to obtain Annona cherimola Mill. extract 2.

[0061] A preparation method of Annona cherimola Mill. extract 3, comprising the following steps:

[0062] The fruits of Annona cherimola Mill. were dried to a constant weight at 50 °C, then crushed and sieved, and added to an ethanol solution (the mass percentage of ethanol was 65%) for ultrasonic extraction. The temperature of ultrasonic extraction was 65 °C, the time was 40 min, the power was 150 W, and the solid-liquid ratio was 1 g: 20 mL. After ultrasonic extraction, the supernatant was collected by centrifugation and concentrated to obtain Annona cherimola Mill. extract 3.

[0063] A preparation method of Annona cherimola Mill. extract 4, comprising the following steps:

[0064] The fruits of Annona cherimola Mill. were dried to a constant weight at 50 °C, then crushed and sieved, and added to an ethanol solution (the mass percentage of ethanol was 55%) for ultrasonic extraction. The temperature of ultrasonic extraction was 45 °C, the time was 30 min, the power was 90 W, and the solid-liquid ratio was 1 g: 15 mL. After ultrasonic extraction, the supernatant was collected by centrifugation and concentrated to obtain Annona cherimola Mill. extract 4.

[0065] Examples 1-17 and Comparative Examples 1-19

[0066] Examples 1-17 and Comparative Examples 1-19 provided a multi-dimensional anti-pollution composition, and its components and parts by weight were shown in Table 1 and Table 2. The preparation method of the multi-dimensional anti-pollution composition comprised the following steps: weighing the preparation raw materials according to parts by weight and mixing them evenly to obtain the multi-dimensional anti-pollution composition.

[0067] Table 1

[0068]

[0069]

[0070] Table 2

[0071]

[0072]

[0073] Comparative Example 20

[0074] Compared with Example 15, the difference lies in the different composition formulas. Annona seed extract is used to replace Annona muricata leaf extract, and the other components, their preparation methods are the same as those in Example 1.

[0075] Effect Example 1: Cell experiments prove the anti-pollution effect of the multi-dimensional anti-pollution composition

[0076] Experimental method:

[0077] This effect example explores the anti-air pollution effects of the multi-dimensional anti-pollution compositions prepared in Examples 1 to 17 and Comparative Examples 1 to 20.

[0078] The cell line used is human keratinocyte HaCaT (Beijing Na Biotechnology), and the test conditions are: the temperature in the incubator is 37±1°C, the humidity is 90±5%, and the carbon dioxide is 5±1%; according to the grouping, cell culture and treatment are carried out, and then tests are carried out. The specific tests are CYP1A1 and barrier protein FLG gene detection, and the detection of the content of inflammatory factor IL-6. The test methods are as follows:

[0079] (1) Inoculate the cell suspension into a 96-well cell culture plate, with a density of 2000 cells / well, add 100 μL of culture medium to each well, and culture for 24 h;

[0080] (2) Collect vehicle exhaust and expose the cells to the exhaust gas for 2 h;

[0081] (3) Discard the supernatant. In the blank control group, add 100 μL of DMEM medium (2105341, Gibco), and add 100 μL of DMEM medium containing 0.01% of the corresponding sample of the multi-dimensional anti-pollution compositions prepared in Examples 1 to 17 and Comparative Examples 1 to 20 respectively;

[0082] (4) After incubating for 22 h, collect the culture medium and cells respectively;

[0083] (5) CYP1A1 and barrier protein FLG gene detection: Use an RNA extraction kit (TRIzol, Thermo Fisher) to extract the RNA of the cells, and use a reverse transcription kit to reverse transcribe the RNA into cDNA (RevertAid RT, Thermo Fisher). Use the synthesized cDNA as a template for real-time fluorescence quantitative PCR reaction (TaqMan, Thermo Fisher). The real-time fluorescence quantitative PCR method is used to detect the CYP1A1 and FLG gene expression levels of HaCaT cells in each group. Use GAPDH as an internal reference gene. The CYP1A1 primer sequences used are 5’-CTTCATCCTGGAGACCTTCC-3‘, 5’-AAGACCTCCCAGCGGGCAA-3‘, and the FLG primer sequences are 5’-TGAAGCCTATGACACCACTGA-3‘, 5’-TCCCCTACGCTTTCTTGTCCT

[0084] -3‘, the GAPDH primer sequences used were 5’-ACTTTGTCAAGCTCATTTCC-3‘ and 5’-TGCAGCGAACTTTATTGATG-3‘.

[0085] (6) Detection of inflammatory factor IL-6: The human IL-6 ELISA kit (EHC007.96, Shenzhen Xinbosheng Biotechnology Co., Ltd.) was used to detect the content of inflammatory factor IL-6 released by cells in the culture medium.

[0086] PM2.5 in the air can activate the aryl hydrocarbon receptor AHR on the skin, damage the skin barrier, and induce skin inflammation, marked by up-regulation of the CYP1A1 gene expression of the aryl hydrocarbon receptor activation signal, reduction of barrier proteins, and increase of inflammatory factors. Therefore, after PM2.5 treatment, the lower the CYP1A1 gene expression level, the more barrier proteins, and the fewer cell inflammatory factors, the stronger the anti-pollution ability of the anti-pollution composition. The anti-pollution ability of the anti-pollution composition is represented by the improvement of CYP1A1, the barrier protection ability is represented by the improvement of FLG, and the anti-inflammatory ability is represented by the improvement of IL-6. The formulas are as follows:

[0087] Improvement rate of CYP1A1 = 1 - CYP1A1 expression level 样品处理 / CYP1A1 expression level 空白对照 * 100%;

[0088] Improvement rate of FLG = FLG expression level 样品处理 / FLG expression level 空白对照 * 100% - 1;

[0089] Improvement rate of IL-6 = 1 - IL-6 content 样品处理 / IL-6 content 空白对照 * 100%

[0090] As shown in Table 3 of the results.

[0091] Table 3

[0092]

[0093]

[0094] Results: As can be seen from Table 3, the multi-dimensional anti-pollution compositions prepared in Examples 1 to 17 of the present application have strong anti-pollution ability, barrier protection ability, and anti-inflammatory ability, and can effectively isolate pollutants or repair skin damage for a long time.

[0095] Among them, compared with Example 6, Examples 7 to 9 changed the ultrasonic extraction conditions of the Annona muricata extract. The anti-pollution effects of the multi-dimensional anti-pollution compositions in Examples 6 to 8 were better than those in Example 9, indicating that the ultrasonic extraction temperature (50 - 65 °C) and power (100 - 150 W) of the present application are beneficial to improving the extraction efficiency of the Annona muricata extract, and can more efficiently prepare a higher-purity Annona muricata extract in a shorter time, thereby making the anti-pollution ability, barrier protection ability and anti-inflammatory ability of the multi-dimensional anti-pollution composition better.

[0096] Compared with Examples 1 to 6, in Examples 10 to 15, extracts of Vaccaria segetalis, Adansonia digitata pulp and Terminalia chebula fruits were added and compounded with resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide and Annona muricata extract, which can further promote the multi-dimensional anti-pollution composition to resist various pollutants such as air pollution, ultraviolet rays and heavy metals, and comprehensively improve the effects of soothing, repairing and whitening of the multi-dimensional anti-pollution composition.

[0097] Examples 16 to 17 were inferior to Examples 10 to 15, indicating that the specific dosages of the extracts of Vaccaria segetalis, Adansonia digitata pulp and Terminalia chebula fruits have a greater impact on the anti-air pollution ability. When an excessive amount of the extracts of Vaccaria segetalis and Adansonia digitata pulp was added in Example 17, it would affect the synergistic effect of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide and Annona muricata extract. Therefore, the specific dosages of the extracts of Vaccaria segetalis and Adansonia digitata pulp can better improve the anti-air pollution ability of the multi-dimensional anti-pollution composition.

[0098] Compared with Example 15, when one of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide and Annona muricata extract was removed from the multi-dimensional anti-pollution compositions of Comparative Examples 1 to 4, the anti-pollution ability, barrier protection ability and anti-inflammatory ability of the composition were inferior to those of Example 15. When the total weight of the components remained unchanged, when at least one of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide and Annona muricata extract was removed from the multi-dimensional anti-pollution compositions of Comparative Examples 5 to 15, or when the Annona muricata extract was replaced with Annona squamosa seed extract in Comparative Example 20, the effect of the air pollution resistance ability was inferior to that of Example 15, indicating that when the components of the multi-dimensional anti-pollution composition of the present application are synergistically combined, the anti-pollution ability, barrier protection ability and anti-inflammatory ability of the multi-dimensional anti-pollution composition can be improved.

[0099] The compositions of Comparative Example 16 did not contain the extracts of Vaccaria segetalis and Adansonia digitata pulp, and Comparative Example 17 did not contain the extract of Terminalia chebula fruit, and the results were inferior to those of Example 15, indicating that adding the extracts of Vaccaria segetalis, Adansonia digitata pulp and Terminalia chebula fruit can better improve the anti-air pollution ability of the composition.

[0100] In Comparative Examples 18 to 19, the mass ratio ranges of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and Annona muricata extract are not within the scope of this application, and the air pollution resistance of the composition is poor.

[0101] Effect Example 2: Human Skin Patch Test

[0102] The multi-dimensional anti-pollution compositions prepared in Examples 1 to 17 and Comparative Examples 1 to 20 were formulated into anti-pollution emulsions as shown in Table 4. Among them, the multi-dimensional anti-pollution compositions in Application Examples 1 to 17 were respectively the multi-dimensional anti-pollution compositions prepared in Examples 1 to 17; the multi-dimensional anti-pollution compositions in Comparative Application Examples 1 to 20 were respectively the multi-dimensional anti-pollution compositions prepared in Comparative Examples 1 to 20; for example, the multi-dimensional anti-pollution composition used in Application Example 1 was the multi-dimensional anti-pollution composition in Example 1, the multi-dimensional anti-pollution composition used in Application Example 8 was the multi-dimensional anti-pollution composition in Example 8, the multi-dimensional anti-pollution composition used in Application Example 17 was the multi-dimensional anti-pollution composition in Example 17, and so on; the multi-dimensional anti-pollution compositions used in Application Examples 18 to 19 were the multi-dimensional anti-pollution compositions prepared in Example 15.

[0103] Table 4 Anti-pollution Emulsion Formulation

[0104]

[0105]

[0106] The present application provides a preparation method of an anti-pollution emulsion, and the preparation method includes the following steps:

[0107] (1) Mix and stir a humectant, a thickener, and a part of a pH regulator (disodium EDTA) with water, heat to 85 °C, and then homogenize at a rotation speed of 1300 rpm for 4 min. After homogenization, keep it warm for standby to obtain a prefabricated component A;

[0108] (2) Mix emulsifiers, heat to 75 °C, and then homogenize at a rotation speed of 1300 rpm for 4 min. After homogenization, keep it warm for standby to obtain a prefabricated component B;

[0109] (3) Mix preservatives, heat to 60 °C to melt, to obtain a prefabricated component C;

[0110] (4) Heat the prefabricated Component A to 80 °C, add the prefabricated Component B at a rotation speed of 300 rpm, stir and mix, then cool down to 60 °C, add the prefabricated Component C at a rotation speed of 300 rpm and stir and mix. Subsequently, cool down to below 45 °C, add the multi-dimensional anti-pollution composition and continue stirring for 8 min. Finally, add the remaining pH regulator (arginine) to adjust the pH to 5.5 - 7.0, then stop stirring, discharge the material, and obtain the anti-pollution emulsion.

[0111] Recruit 30 volunteers, 15 males and 15 females, aged 20 - 50 years old. Using the closed patch test method, place an equal amount (0.020 mL - 0.025 mL) of the test sample (the anti-pollution emulsion containing 20% w / w multi-dimensional anti-pollution composition) in a specific patch tester, apply it to the volunteers' arms with a low-sensitization tape, gently press to make it evenly adhere to the skin, and leave it for 24 h; the blank control group is distilled water, and the blank application example is the emulsion without the anti-pollution composition. After 24 h, remove the patch tester, and observe the skin reaction at 0.5 h, 24 h, and 48 h, and record the results. The adverse reaction grades of the skin are shown in Table 5 below.

[0112] Table 5

[0113]

[0114] After testing, the anti-pollution emulsions containing the multi-dimensional anti-pollution composition provided in Examples 1 - 17 and Comparative Examples 1 - 20 of the present application were all negative reactions after the human patch test, and they were safe and non-irritating to human skin.

[0115] Effect Example Three: Human Efficacy Test for Soothing, Repairing, and Removing Sallow Complexion (Whitening)

[0116] Experimental method: According to the "Technical Specifications for Cosmetics Safety" (2015), select 222 Asian adult testers with sensitive skin aged 18 - 60 years old, randomly divide them into 37 groups, with 6 people in each group. The volunteers use the sample (the anti-pollution emulsion of the multi-dimensional anti-pollution composition according to Table 3) or the blank application example (the anti-pollution emulsion without the multi-dimensional anti-pollution composition) on the whole face once in the morning and once in the evening every day. Data is collected when they visit on the 0th day and the 7th day. After the volunteers visit, wash their faces with facial cleanser, sit still in an air-conditioned room at a temperature of 21 ± 1 °C and a humidity of 50 ± 10% for 20 minutes, then use Visia-7 to take facial photos, and perform image analysis on the exported red area pictures to obtain the a* value; use a skin moisture loss test probe ( TM Hex) to measure the trans-epidermal water loss value TEWL of the cheekbones; use a CK skin color test probe CL400 to measure the yellowness b* value of the cheekbones.

[0117] The skin soothing ability is represented by the improvement of the a* value, the skin barrier repair ability is represented by the improvement of the TEWL value, and the skin yellowing reduction ability is represented by the improvement of the b* value. The formula is as follows: Improvement rate = [((a value on day 0 of the sample - a value on day 7 of the sample) / a value on day 0 of the sample) - ((a value on day 0 of the blank application example - a value on day 7 of the blank application example) / a value on day 0 of the blank application example)] * 100%.

[0118] The results are shown in Table 6.

[0119] Table 6

[0120]

[0121]

[0122] As can be seen from the above table, when the multi-dimensional anti-pollution composition prepared in the embodiments of the present application is prepared into an anti-pollution emulsion (Application Examples 1 to 19), the improvement rate of the a* value is more than 20.9%, the improvement rate of the TEWL value is more than 15.2%, and the improvement rate of the b* value is more than 5.5%.

[0123] In Application Examples 6 to 9 of the present application, the ultrasonic extraction conditions of the annona muricata extract are different. The improvement rates of the a* value, TEWL value, and b* value in Application Examples 6 to 8 are better than those in Example 9, indicating that using the ultrasonic extraction temperature (50 - 65 °C) and power (100 - 150 W) of the present application is beneficial to improving the extraction efficiency of the annona muricata extract, enabling the prepared anti-pollution emulsion to better achieve multiple functions such as anti-pollution, antioxidant, soothing, repair, and improvement of skin yellowing.

[0124] In Application Examples 10 to 17, extracts of vaccaria segetalis, adansonia digitata pulp, and terminalia chebula fruits are added and compounded with resveratrol oxide, epigallocatechin gallate glucoside, chitosan acetylamine, and annona muricata extract, enhancing multiple functions such as soothing, repair, and improvement of skin yellowing. Application Examples 10 to 15 are better than Application Examples 16 to 17, indicating that when the extracts of vaccaria segetalis, adansonia digitata pulp, and terminalia chebula fruits are combined in a specific mass ratio, the anti-pollution emulsion has better multiple functions such as soothing, repair, and improvement of skin yellowing.

[0125] When one of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract is removed in Comparative Application Examples 1 to 4, or when at least one of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract is removed in Comparative Application Examples 5 to 15 while the total weight of the components remains unchanged, or when the annona muricata extract in Comparative Application Example 20 is replaced with annona squamosa seed extract, the anti-pollution emulsion is inferior to Application Example 15 in terms of soothing, repairing, and whitening effects. It shows that only when the multi-dimensional anti-pollution composition of the present application adopts the components of the present application and their specific mass ratio in a synergistic combination can it better improve the multiple functions of the product such as soothing, repairing, and improving skin sallowness.

[0126] The composition of Comparative Application Example 16 does not contain semen vaccariae extract and adansonia digitata pulp extract, and Comparative Application Example 17 does not contain terminalia chebula fruit extract. The results are inferior to Application Example 15, indicating that adding semen vaccariae extract, adansonia digitata pulp extract, and terminalia chebula fruit extract can better improve the soothing, repairing, and whitening effects of the product. In Comparative Application Examples 18 to 19, the mass ratio range of resveratrol oxide, epigallocatechin gallate glucoside, chitosan oligosaccharide, and annona muricata extract is not within the scope of the present application, and the soothing, repairing, and skin sallowness improvement effects of the product are poor.

[0127] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application rather than to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present application can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A multidimensional anti-pollution composition, characterized in that: The multidimensional anti-pollution composition comprises oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract; The mass ratio of the oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract is (0.1-3): (0.001-0.1): (1-10): (0.1-5); The multidimensional anti-pollution composition also includes Vaccaria segetalis extract, Adansonia baobab pulp extract and Terminalia chebula fruit extract; The mass ratio of the cherimoya extract, the vaccaria extract, the baobab pulp extract and the terminalia chebula fruit extract is (1-2): (0.1-3): (0.1-3): (0.01-1); The preparation method of the cherimoya extract comprises the following steps: The fruit of the custard apple is crushed and sieved, and then added into an ethanol solution for ultrasonic extraction. After the ultrasonic extraction is completed, the supernatant is collected by centrifugation and concentrated to obtain the custard apple extract.

2. The multidimensional anti-pollution composition according to claim 1, characterized in that: The mass ratio of oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract is (0.2-2): (0.005-0.05): (4-8): (0.5-3).

3. The multidimensional anti-pollution composition according to claim 2, characterized in that: The mass ratio of oxidized resveratrol, epigallocatechin gallate glucoside, acetyl glucosamine and cherimoya extract is (0.5-1): (0.01-0.02): (6-7): (1-2).

4. The multidimensional anti-pollution composition according to claim 1, characterized in that The mass ratio of the Annona chebula extract, the Vaccaria segetalis extract, the Adansonia digitata pulp extract and the Terminalia chebula fruit extract is (1-2): (0.5-2): (0.5-2): (0.05-0.5).

5. The multidimensional anti-pollution composition according to claim 4, characterized in that: The mass ratio of the Annona chebula extract, the Vaccaria segetalis extract, the Adansonia digitata pulp extract and the Terminalia chebula fruit extract is (1-2): (1-1.5): (1-1.5): (0.1-0.2).

6. The multidimensional anti-pollution composition according to claim 1, characterized in that: During the ultrasonic extraction, the temperature is 50-65°C, the time is 20-40 minutes, and the power is 100-150W.

7. The multidimensional anti-pollution composition according to claim 1, characterized in that: In the ethanol solution, the mass percentage of ethanol is 55-65%; The solid-liquid ratio in ultrasonic extraction is 1g:(10~20)mL.

8. Use of the multidimensional anti-pollution composition according to any one of claims 1 to 7 in the preparation of cosmetics.

9. A cosmetic, characterized in that: It comprises the multidimensional anti-pollution composition according to any one of claims 1 to 7; the mass percentage of the multidimensional anti-pollution composition in the cosmetics is 1 to 20%.

10. The cosmetic according to claim 9, characterized in that The cosmetic is an emulsion; the emulsion also includes the following components in percentage by mass: 0.05-0.3% thickener, 0.5-1% moisturizer, 0.01-0.3% pH regulator, 0.5-5% emulsifier and 0.5-3% preservative.

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