An anti-aging composition and its preparation method

Abstract

The present invention discloses an anti-aging composition and a preparation method thereof, belonging to the technical field of cosmetics. The anti-aging composition provided by the present invention comprises the following components in parts by mass: 0.001-0.01 part of freeze-dried powder of porcine umbilical cord mesenchymal stem cell secretions, 15-20 parts of extract of leaves of Argania spinosa, 10-13 parts of extract of Eucheuma muricatum, and 1-3 parts of extract of adventitious roots of Apostichopus japonicus. The anti-aging composition provided by the present invention has the effects of anti-aging and improving the migration rate of skin cells, and is overall mild and non-irritating. Applying the anti-aging composition of the present invention to cosmetics can effectively anti-aging, reduce wrinkles, improve skin elasticity and increase the water content of the stratum corneum. In addition, the components of the composition provided by the present invention are derived from natural sources, and the preparation method is simple, which is suitable for actual production.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging composition and a preparation method thereof. Background Art

[0002] Aging is a continuous process. As people age, they will inevitably age. The skin is the largest organ in the human body. Long-term exposure to the environment and various endogenous and exogenous stimuli will accelerate the aging process. During the skin aging process, other skin problems often occur, such as skin oxidation, low cell mobility, insufficient nutrition of skin cells, slow cell metabolism, etc., which lead to failure of the skin barrier. Therefore, in the anti-aging process, it is necessary to replenish water and adjust the health of the skin at the same time. Therefore, it is necessary to provide an anti-aging product with multiple functions such as anti-oxidation and improving skin cell mobility to solve a series of problems that may arise during the skin aging process. Summary of the invention

[0003] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and thereby provide an anti-aging composition which can effectively improve skin elasticity, enhance skin cell mobility, have anti-aging effects and is mild and non-irritating.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] In a first aspect, the present invention provides an anti-aging composition, comprising the following components in parts by weight:

[0006]

[0007] The present invention selects to add appropriate mass proportions of porcine umbilical cord mesenchymal stem cell secretory factor freeze-dried powder, Argania spinulosa leaf extract, Eucheuma spinulosa extract, and Apostichopus japonicus adventitious root extract to the anti-aging composition. These four components can cooperate with each other and synergize to solve the problems of skin sagging and aging caused by external environmental factors. Specifically, the components of the anti-aging composition provided by the present invention cooperate with each other, and after use, they can effectively provide the skin with the effects of improving skin elasticity, anti-oxidation, and enhancing skin cell mobility.

[0008] Porcine umbilical cord mesenchymal stem cell secretion factors contain a variety of cytokines such as SCF, TGF-β, KGF, IGF, FGF, HGF, VEGF, SOD, SIRT, etc. These cytokines play a series of anti-aging and skin barrier repairing effects through different mechanisms, such as promoting cell proliferation and differentiation, regulating immune response, promoting cell chemotaxis, regulating cell apoptosis and regulating inflammatory response.

[0009] In addition, Eucheuma spinulosa extract, Sea Cucumber adventitious root extract and Argania spinulosa leaf extract are rich in polyphenols and flavonoids, which have many positive effects on the skin such as anti-oxidation, moisturizing, nourishing, repairing, anti-inflammatory and antibacterial.

[0010] This study found that the combined use of the above components can synergistically enhance the effects and achieve excellent antioxidant and anti-aging effects.

[0011] As a preferred embodiment of the anti-aging composition of the present invention, the anti-aging composition comprises the following components in parts by weight:

[0012]

[0013] As the most preferred embodiment of the anti-aging composition of the present invention, the anti-aging composition comprises the following components by weight:

[0014]

[0015] The present invention has found that the mass fraction of each component in the anti-aging composition will affect the performance of the product. When the mass fraction of the components is further selected to be within the above range (including the endpoint value), the obtained product can more significantly improve the skin's elasticity, anti-oxidation, skin cell mobility and other effects.

[0016] As a preferred embodiment of the anti-aging composition of the present invention, the preparation method of the porcine umbilical cord mesenchymal stem cell secretory factor lyophilized powder is to add porcine umbilical cord mesenchymal stem cells to a specific culture medium for culturing, and the specific steps are as follows:

[0017] S1. Pig umbilical cord mesenchymal stem cells were cultured at 1×10 6 The cells were inoculated in a specific culture medium and incubated at 37°C with 5% CO 2 Cultivate in an incubator, and when the porcine umbilical cord mesenchymal stem cells are close to 90% confluent, subculture, and change the medium every 12 hours;

[0018] S2, amplify the porcine umbilical cord mesenchymal stem cells to the fifth generation, collect the fifth generation porcine umbilical cord mesenchymal stem cells, and culture the fifth generation porcine umbilical cord mesenchymal stem cells at 1×10 6 The cells were inoculated into a specific culture medium containing 0.5 v / v% 1 mg / mL quercetin, cultured for 18 h, and then centrifuged to collect the supernatant, which was filtered with a 0.22 μm sterile filter, and then freeze-dried to obtain freeze-dried powder of porcine umbilical cord mesenchymal stem cell secretin.

[0019] The specific culture medium is DEME low-glucose complete culture medium supplemented with 1 v / v % NEAA.

[0020] The porcine umbilical cord mesenchymal stem cells were purchased from Shanghai Bolson Biotechnology Co., Ltd. with the product number BES2019PP.

[0021] The present invention has found that, during the culture process of pig umbilical cord mesenchymal stem cells, adding 0.5 v / v% quercetin can effectively increase the secretion of secretin from pig umbilical cord mesenchymal stem cells.

[0022] In a second aspect, the present invention provides a cosmetic comprising the anti-aging composition of the present invention.

[0023] As a preferred embodiment of the cosmetics of the present invention, the cosmetics are composed of the following components in percentage by mass:

[0024]

[0025] The balance was deionized water.

[0026] As a preferred embodiment of the cosmetics of the present invention, the cosmetics include any one of lotion, emulsion, cream, mask, essence, and spray.

[0027] In a third aspect of the present invention, the present invention provides a method for preparing a cosmetic, the preparation method specifically comprising the following steps:

[0028] T1: Mix EDTA-2Na, deionized water and glycerol evenly and stir for 6-10 minutes until they are completely dissolved to obtain product 1;

[0029] T2: heat isopropyl myristate, cetearyl alcohol, and polyglyceryl-2 distearate to 80°C and mix well to obtain product 2;

[0030] T3: Mix product 1 and product 2 evenly, cool to 40° C., add the anti-aging composition and phenoxyethanol, stir evenly, and discharge to obtain the cosmetics.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The present invention provides an anti-aging composition, in which four components can cooperate with each other and synergistically enhance the effect, thereby achieving anti-aging, improving the migration rate of skin cells, and being overall mild and non-irritating.

[0033] Specifically, firstly, the present invention provides a method for preparing freeze-dried powder of porcine umbilical cord mesenchymal stem cell secretome, and the secretion amount of porcine umbilical cord mesenchymal stem cell secretome can be effectively increased by adding quercetin to the culture medium.

[0034] Secondly, the present invention provides an anti-aging composition containing the porcine umbilical cord mesenchymal stem cell secretory factor lyophilized powder prepared by the present invention. The composition can maximize its anti-aging effects, skin cell migration rate enhancement effects, etc. within the addition range specified by the present invention through the mutual use and synergistic enhancement of the four components thereof.

[0035] Furthermore, the anti-aging composition provided by the present invention is mild and non-irritating as a whole, has a wide range of applicable objects, and has a simple preparation method, and is suitable for actual production. DETAILED DESCRIPTION

[0036] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in "secretin", "composition" and "application example" are intended to describe specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0037] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the percentages and mass fractions are calculated.

[0038] Some raw materials and their sources are as follows:

[0039] Porcine umbilical cord mesenchymal stem cells: purchased from Shanghai Bolsen Biotechnology Co., Ltd., catalog number BES2019PP;

[0040] Argania spinulosa leaf extract: purchased from Shaanxi Mufan Biotechnology Co., Ltd., MF-00083;

[0041] Eucheuma spinulosa extract: purchased from Yangling Ciyuan Biotechnology Co., Ltd.;

[0042] Northeast Sea Cucumber adventitious root extract: purchased from Saiye (Guangzhou) Biotechnology Co., Ltd.;

[0043] Quercetin: purchased from Jinjinle (Hunan) Chemical Co., Ltd., product number B20527;

[0044] DMEM low-glucose complete medium: purchased from Shanghai Bolson Biotechnology Co., Ltd., catalog number BES2003CMA;

[0045] NEAA: purchased from Shanghai Boersen Biotechnology Co., Ltd., product number BES2000C.

[0046] Preparation of porcine umbilical cord mesenchymal stem cell secretin lyophilized powder:

[0047] Secretin lyophilized powder 1:

[0048] S1, pig umbilical cord mesenchymal stem cells were divided into 1*10 6 The cells were inoculated in a specific culture medium at 37°C and 5% CO2 Cultivate in an incubator, and when the porcine umbilical cord mesenchymal stem cells are close to 90% confluent, subculture, and change the medium every 12 hours;

[0049] S2, amplify the porcine umbilical cord mesenchymal stem cells to the fifth generation, collect the fifth generation porcine umbilical cord mesenchymal stem cells, and multiply the fifth generation porcine umbilical cord mesenchymal stem cells by 1*10 6 The cells were inoculated into a specific culture medium containing 0.5 v / v% quercetin, cultured for 18 h, and then centrifuged to collect the supernatant, which was filtered with a 0.22 μm sterile filter, and then freeze-dried to obtain freeze-dried powder of porcine umbilical cord mesenchymal stem cell secretin.

[0050] The quercetin solution was 1 mg / mL.

[0051] Secretin freeze-dried powder①:

[0052] The difference from the secretin lyophilized powder 1 is that the specific culture medium containing 0.5 v / v% 1 mg / mL quercetin in S2 is replaced with a specific culture medium.

[0053] Secretin lyophilized powder②:

[0054] Different from the secretin 1 lyophilized powder, the specific medium containing 0.5 v / v% 1 mg / mL quercetin in S2 was replaced with a specific medium containing 0.5 v / v% 1 mg / mL rutin.

[0055] Secretin freeze-dried powder ③:

[0056] The difference from the secretin lyophilized powder 1 is that the specific culture medium containing 0.5 v / v% 1 mg / mL of quercetin in S2 is replaced with a specific culture medium containing 0.5 v / v% 1 mg / mL of proanthocyanidins.

[0057] Secretin freeze-dried powder④:

[0058] Different from the secretin lyophilized powder 1, the specific culture medium containing 0.5 v / v% 1 mg / mL of quercetin in S2 is replaced with a specific culture medium containing 0.5 v / v% 1 mg / mL of morin.

[0059] Secretin freeze-dried powder⑤:

[0060] Different from the secretin 1 lyophilized powder, the specific medium containing 0.5 v / v% 1 mg / mL quercetin in S2 was replaced with a specific medium containing 1 v / v% 1 mg / mL quercetin.

[0061] The mass proportions of the components in the anti-aging composition are as follows:

[0062] Composition 1:

[0063] 0.005 parts of porcine umbilical cord mesenchymal stem cell secretin freeze-dried powder (secretin freeze-dried powder 1), 18 parts of Argania spinulosa leaf extract, 12 parts of Eucheuma spinulosa extract, and 2 parts of Sea Cucumber adventitious root extract.

[0064] Composition 2:

[0065] 0.001 parts of porcine umbilical cord mesenchymal stem cell secretin freeze-dried powder (secretin freeze-dried powder 1), 20 parts of Argania spinulosa leaf extract, 13 parts of Eucheuma spinulosa extract, and 3 parts of Sea Cucumber adventitious root extract.

[0066] Composition 3:

[0067] 0.01 parts of porcine umbilical cord mesenchymal stem cell secretin freeze-dried powder (secretin freeze-dried powder 1), 15 parts of Argania spinulosa leaf extract, 10 parts of Eucheuma spinulosa extract, and 1 part of Sea Cucumber adventitious root extract.

[0068] Composition ①:

[0069] 18 parts of Argania spinulosa leaf extract, 12 parts of Eucheuma spinulosa extract, and 2 parts of Sea Cucumber adventitious root extract.

[0070] Composition ②:

[0071] 0.0005 parts of porcine umbilical cord mesenchymal stem cell secretin freeze-dried powder (secretin freeze-dried powder 1), 12 parts of Eucheuma spinulosa extract, and 2 parts of adventitious root extract of Sea Cucumber japonicus.

[0072] Composition ③:

[0073] 0.0005 parts of porcine umbilical cord mesenchymal stem cell secretin freeze-dried powder (secretin freeze-dried powder 1), 18 parts of Argania spinulosa leaf extract, and 2 parts of sea cucumber adventitious root extract.

[0074] Composition ④:

[0075] 0.0005 parts of porcine umbilical cord mesenchymal stem cell secretin freeze-dried powder (secretin freeze-dried powder 1), 18 parts of Argania spinulosa leaf extract, and 12 parts of Eucheuma spinulosa extract.

[0076] Composition ⑤:

[0077] 0.0005 parts of porcine umbilical cord mesenchymal stem cell secretin freeze-dried powder (secretin freeze-dried powder 1).

[0078] Composition ⑥:

[0079] 18 parts of Argania spinulosa leaf extract.

[0080] Composition ⑦:

[0081] 12 parts of Eucheuma spinulosa extract.

[0082] Composition ⑧:

[0083] 2 parts of adventitious root extract of Sea Cucumber japonicus.

[0084] In the above compositions ①-⑧, the missing parts by mass of raw materials are made up with deionized water.

[0085] The cosmetic raw materials and mass percentages are as follows:

[0086] Application Example 1: 2 wt% of anti-aging composition (composition 1); 5 wt% of glycerol; 3 wt% of isopropyl myristate; 2 wt% of cetearyl alcohol; 0.2 wt% of EDTA-2Na; 0.7 wt% of polyglyceryl-2 dipolyhydroxystearate; 0.8 wt% of phenoxyethanol; the balance is deionized water.

[0087] Application Example 2: 1 wt% anti-aging composition (composition 2); 4 wt% glycerol; 4 wt% isopropyl myristate; 1 wt% cetearyl alcohol; 0.1 wt% EDTA-2Na; 1 wt% polyglyceryl-2 dipolyhydroxystearate; 0.1 wt% phenoxyethanol; the balance is deionized water.

[0088] Application Example 3: Anti-aging composition 3wt% (Composition 3); 6wt% glycerol; 2wt% isopropyl myristate; 2wt% cetearyl alcohol; 0.3wt% EDTA-2Na; 0.5wt% polyglyceryl-2 dipolyhydroxystearate; 1wt% phenoxyethanol; the balance is deionized water.

[0089] Application Example ①: anti-aging composition 2wt% (composition ①); glycerin 5wt%; isopropyl myristate 3wt%; cetearyl alcohol 2wt%; EDTA-2Na 0.2wt%; polyglyceryl-2 dipolyhydroxystearate 0.7wt%; phenoxyethanol 0.8wt%; the balance is deionized water.

[0090] Application Example ②: anti-aging composition 2wt% (composition ②); glycerin 5wt%; isopropyl myristate 3wt%; cetearyl alcohol 2wt%; EDTA-2Na 0.2wt%; polyglyceryl-2 dipolyhydroxystearate 0.7wt%; phenoxyethanol 0.8wt%; the balance is deionized water.

[0091] Application Example ③: anti-aging composition 2wt% (composition ③); glycerin 5wt%; isopropyl myristate 3wt%; cetearyl alcohol 2wt%; EDTA-2Na 0.2wt%; polyglyceryl-2 dipolyhydroxystearate 0.7wt%; phenoxyethanol 0.8wt%; the balance is deionized water.

[0092] Application Example ④: anti-aging composition 2wt% (composition ④); glycerol 5wt%; isopropyl myristate 3wt%; cetearyl alcohol 2wt%; EDTA-2Na 0.2wt%; polyglycerol-2 dipolyhydroxystearate 0.7wt%; phenoxyethanol 0.8wt%; the balance is deionized water.

[0093] Application Example ⑤: anti-aging composition 2wt% (composition ⑤); glycerin 5wt%; isopropyl myristate 3wt%; cetearyl alcohol 2wt%; EDTA-2Na 0.2wt%; polyglyceryl-2 dipolyhydroxystearate 0.7wt%; phenoxyethanol 0.8wt%; the balance is deionized water.

[0094] Application Example ⑥: anti-aging composition 2wt% (composition ⑥); glycerol 5wt%; isopropyl myristate 3wt%; cetearyl alcohol 2wt%; EDTA-2Na 0.2wt%; polyglyceryl-2 dipolyhydroxystearate 0.7wt%; phenoxyethanol 0.8wt%; the balance is deionized water.

[0095] Application Example ⑦: anti-aging composition 2wt% (composition ⑦); glycerin 5wt%; isopropyl myristate 3wt%; cetearyl alcohol 2wt%; EDTA-2Na 0.2wt%; polyglyceryl-2 dipolyhydroxystearate 0.7wt%; phenoxyethanol 0.8wt%; the balance is deionized water.

[0096] Application Example ⑧: anti-aging composition 2wt% (composition ⑧); glycerin 5wt%; isopropyl myristate 3wt%; cetearyl alcohol 2wt%; EDTA-2Na 0.2wt%; polyglyceryl-2 dipolyhydroxystearate 0.7wt%; phenoxyethanol 0.8wt%; the balance is deionized water.

[0097] Blank application example: 5wt% glycerol; 3wt% isopropyl myristate; 2wt% cetearyl alcohol; 0.2wt% EDTA-2Na; 0.7wt% polyglyceryl-2 dipolyhydroxystearate; 0.8wt% phenoxyethanol; the balance is deionized water.

[0098] The preparation method of Application Example 1 is specifically as follows:

[0099] T1: Mix EDTA-2Na, deionized water and glycerol evenly and stir for 6-10 minutes until they are completely dissolved to obtain product 1;

[0100] T2: heat isopropyl myristate, cetearyl alcohol, and polyglyceryl-2 distearate to 80°C and mix well to obtain product 2;

[0101] T3: Mix product 1 and product 2 evenly, cool to 40° C., add the anti-aging composition and phenoxyethanol, stir evenly, and discharge to obtain the cosmetics.

[0102] The preparation methods of Application Examples 2-3, Application Examples ①-⑧, and Blank Application Examples were consistent with that of Application Example 1, and the missing component masses were filled with deionized water.

[0103] Test 1: ELISA to detect SCF secretion

[0104] Verify the secretin content in secretin 1 and secretin ①-⑤.

[0105] The detection was performed according to the instructions of the porcine stem cell factor (SCF) ELISA kit (Cat. No.: BES5544K) purchased from Shanghai Bolson Biotechnology Co., Ltd.

[0106] Preparation process of secretin: First, pig umbilical cord mesenchymal stem cells were divided into 1*10 6 The cells were inoculated into a specific culture medium at a density of 100 units and then incubated at 37°C and 5% CO 2 Cultivate in an incubator, and when the porcine umbilical cord mesenchymal stem cells are close to 90% confluent, subculture, and change the medium every 12 hours;

[0107] Then, the pig umbilical cord mesenchymal stem cells were expanded to the fifth generation, and the fifth generation pig umbilical cord mesenchymal stem cells were collected and the fifth generation pig umbilical cord mesenchymal stem cells were cultured at 1*10 6 The cells were inoculated into the culture medium at a unit inoculation density and cultured for 18 h, followed by centrifugation, collection of the supernatant, and filtration of the supernatant using a 0.22 μm sterile filter.

[0108] Among them, the specific culture medium used for secretin 1 and ①-⑤ are the specific culture medium used for secretin lyophilized powder 1 and ①-⑤ in the S1 preparation step, and the culture medium used are the culture medium used for secretin lyophilized powder 1 and ①-⑤ in the S2 preparation step; the blank group is without porcine umbilical cord mesenchymal stem cells, but only with specific culture medium.

[0109] According to the instructions of the porcine stem cell factor (SCF) ELISA kit, SCF in the test group and the blank group was detected.

[0110] Table 1 Effect of culture medium on SCF secretion by porcine umbilical cord mesenchymal stem cells

[0111]

[0112] Based on the experimental results in Table 1, it can be seen that quercetin can effectively increase the secretion of secretin by porcine umbilical cord mesenchymal stem cells. In view of the above results, secretin ①-④ did not participate in subsequent experiments; by comparing secretin 1 with secretin ⑤, it can be seen that high concentrations of quercetin have a certain inhibitory effect on the secretion of SCF by porcine umbilical cord mesenchymal stem cells, so secretin ⑤ did not participate in subsequent experiments.

[0113] Experiment 2: Cell migration assay

[0114] Verify the cell migration effects of Application Examples 1-3, Application Examples ①-⑧, and Blank Application Example.

[0115] The degree of cell migration has an important impact on wound healing and skin repair. In the process of wound healing, cell migration is an important step for epidermal cells to cover the wound, so the higher the cell migration rate, the faster the wound heals. At the same time, cell migration is also involved in the repair process after skin damage. If cell migration is impaired, it will lead to skin repair disorders, which in turn cause various skin diseases.

[0116] Press 1*10 6 Hacat cells were seeded into 12-well plates at a density of 100 units and incubated at 37°C with 5% CO 2 Incubate overnight in an incubator. When the cell plating rate in the 12-well plate reaches about 80%, scratch the sample group and the blank control group. After the scratching, wash the cells with PBS to remove dead cells, then add fresh SCF-free keratinocyte culture medium, add 50μL of 10mg / L according to Application Examples 1-3, Application Examples ①-⑧, and Blank Application Example, and take photos and record using an inverted microscope. 37℃, 5% CO 2 The cells were incubated in an incubator for 24 h, and photographed again for statistical analysis using ImageJ software.

[0117] Table 2 Cell migration rate

[0118] Experimental Group Migration rate / % Application Example 1 55.24 Application Example 2 49.17 Application Example 3 49.24 Application Example ① 35.17 Application Example ② 29.36 Application Example ③ 27.42 Application Example ④ 28.27 Application Example ⑤ 12.51 Application Example ⑥ 9.36 Application Example ⑦ 8.45 Application Example⑧ 8.93 Blank Application Example 6.27

[0119] As can be seen from Table 2, when the technical solution of the present invention is adopted, the obtained composition has a significant cell migration rate. When the raw material components are missing or the porcine umbilical cord mesenchymal stem cell secretome is extracted using an extraction process not specified by the present invention, the cell migration rate of the application example prepared by the obtained composition is significantly reduced.

[0120] Experiment 3: Human efficacy test

[0121] The cosmetics prepared by the present invention were subjected to an 8-week anti-aging test according to the method described in the literature (Zhu Liping et al., Clinical efficacy test and analysis of ginseng anti-aging mask [J], Chinese Journal of Aesthetic Medicine, 2016, 25(02): 33-36).

[0122] Ten volunteers were selected for each application case. Inclusion criteria: ① Male or female, aged 30 to 65 years old; with a certain degree of facial / eye skin sagging or fine wrinkles on the face / eyes; ② Good health, serious attitude, certain expression ability, and can truly reflect the feelings after use; ③ Voluntary participation, signing the informed consent form, strictly abide by the requirements of the research plan, use the product according to the requirements of the research plan and complete follow-up. Exclusion criteria: ① Pregnant or lactating women; ② Sensitive skin; ③ People with damaged facial skin or skin diseases that affect the observation of facial status; ④ People who have received skin treatment, beauty and other tests that may affect the results; ⑤ People who have sunburned or used hormone drugs and immunosuppressants in the past 1 month; ⑥ People who have participated in other facial clinical studies or received treatment by dermatologists currently or in the past 3 months. Termination and exclusion criteria: ① The subject requests to stop the test; ② The subject's compliance is poor; ③ The test cannot be continued due to adverse reactions or special physiological and pathological changes; ④ During the test, other skin care products that affect this experiment are used, and excessive exposure to ultraviolet rays are received.

[0123] Instrument measurement method: The test was conducted using the moisture tester Corneometer, skin moisture loss meter Tewameter, skin elasticity tester MPA580 from German CK Company, and the VISIA facial image analysis system from American Canfield Company.

[0124] ①Measurement of the moisture content of the stratum corneum of the skin (skin moisture content): Use a moisture tester to measure the moisture content of the stratum corneum of the left and right cheeks, and measure 4 times to take the average value;

[0125] ② Measurement of transepidermal water loss (TEWL) of facial skin: Use a skin water loss meter to measure the transepidermal water loss of the left and right faces, and take the average value of the measurement twice;

[0126] ③Facial skin elasticity measurement: Use a skin elasticity tester to measure the skin elasticity at the end of the left and right eyes and under the eyes, and calculate R 2 The average value, R 2 =Ua / Uf; Ua is the recovery value of the skin from the removal of negative pressure to the next continuous test when negative pressure is applied to the skin surface; Uf is the maximum stretching of the skin when negative pressure is present.

[0127] ④ Facial imaging: The VISIA facial image analysis system was used to take photos of the subject's face (front, left and right 45° directions). The wrinkle score and texture score were extracted and the average value was taken.

[0128] Table 3 Human efficacy test results

[0129]

[0130]

[0131] Test results of the water content of the stratum corneum of the skin: As shown in Table 3, compared with the water content of the stratum corneum of the skin in week 0, the water content gradually increased, and the cosmetics prepared by the present invention showed good hydrating and moisturizing effects.

[0132] TEWL value (unit: g / hm 2 ) measurement results: It can be seen from Table 3 that compared with the 0th week, the transepidermal water loss of the skin gradually decreased, and the cosmetics prepared by the present invention showed a good water-locking effect.

[0133] Test results of skin elasticity: As shown in Table 3, the skin elasticity was significantly improved compared with that in week 0, and the cosmetics prepared by the present invention showed a good effect of improving skin elasticity.

[0134] Test results of skin wrinkle scores: As shown in Table 3, compared with the volunteers in week 0, the facial wrinkle scores of the volunteers were significantly and gradually reduced, indicating that the cosmetics prepared by the present invention have a strong effect of improving facial wrinkles and fine lines, and will not produce skin tolerance due to long-term use.

[0135] Therefore, long-term use of the cosmetics prepared by the embodiments of the present invention by the subjects can increase the moisture content of the skin, reduce transepidermal water loss, improve skin elasticity, effectively improve facial wrinkles, and show good anti-aging effects.

[0136] Test 4: Safety test:

[0137] The present invention experiments verify the biosafety of Application Examples 1-3, Application Examples ①-⑧ and blank application examples.

[0138] The 2015 "Technical Specifications for Safety of Cosmetics" was used as a reference standard for the irritation evaluation of cosmetics. The test method was a skin patch test, and 120 randomly distributed people aged 16-65 were tested.

[0139] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0140] Evaluation criteria:

[0141] Grade 0: negative reaction;

[0142] Grade 1: Suspicious reaction, only slight erythema;

[0143] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0144] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0145] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0146] Test results: Skin reactions were negative in all subjects.

[0147] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. An anti-aging composition, characterized in that: The anti-aging composition is composed of the following components in parts by weight: Porcine umbilical cord mesenchymal stem cell secretin lyophilized powder 0.001-0.01 parts; 15-20 parts of Argania spinulosa leaf extract; 10-13 parts of Eucheuma spinulosa extract; 1-3 parts of adventitious root extract of sea cucumber; The preparation method of the porcine umbilical cord mesenchymal stem cell secretin lyophilized powder is as follows: S1. Inoculate porcine umbilical cord mesenchymal stem cells in a specific culture medium, and then culture them in a 37°C, 5% CO2 incubator. When the porcine umbilical cord mesenchymal stem cells are close to 90% fused, subculture them, and change the medium every 12 hours. S2, amplifying the porcine umbilical cord mesenchymal stem cells to the fifth generation, collecting the fifth generation porcine umbilical cord mesenchymal stem cells, inoculating the fifth generation porcine umbilical cord mesenchymal stem cells into a specific culture medium containing 0.5 v / v% quercetin, culturing for 18 hours, and then centrifuging and collecting the supernatant, filtering the supernatant with a 0.22 μm sterile filter, and then freeze-drying the filtrate to obtain porcine umbilical cord mesenchymal stem cell secretory factor lyophilized powder; The specific culture medium is DEME low-glucose complete culture medium supplemented with 1 v / v% NEAA; The quercetin is a 1 mg / mL quercetin aqueous solution.

2. The anti-aging composition according to claim 1, characterized in that: The porcine umbilical cord mesenchymal stem cells were purchased from Shanghai Bolson Biotechnology Co., Ltd. with the product number BES2019PP.

3. The anti-aging composition according to claim 1, characterized in that: The anti-aging composition is composed of the following components in parts by weight: Porcine umbilical cord mesenchymal stem cell secretin freeze-dried powder 0.001-0.005 parts; 15-18 parts of Argania spinulosa leaf extract; 10-12 parts of Eucheuma spinulosa extract; 1-2 parts of adventitious root extract of Sea Cucumber japonicus.

4. The anti-aging composition according to claim 1, characterized in that: The anti-aging composition is composed of the following components in parts by weight: 0.005 parts of porcine umbilical cord mesenchymal stem cell secretin lyophilized powder; Argania spinulosa leaf extract 18 parts; 12 parts of Eucheuma spinulosa extract; 2 parts of adventitious root extract of Sea Cucumber japonicus.

5. A cosmetic, characterized in that: An anti-aging composition comprising the anti-aging composition according to any one of claims 1 to 4.

6. The cosmetic according to claim 5, characterized in that: The cosmetics are composed of the following components in percentage by weight: Anti-aging composition 1-3wt%; Glycerol 4-6wt%; Isopropyl myristate 2-4wt%; Cetearyl alcohol 1-2wt%; EDTA-2Na 0.1-0.3wt%; Polyglyceryl-2 dipolyhydroxystearate 0.5-1wt%; Phenoxyethanol 0.5-1wt%; The balance was deionized water.

7. A method for preparing a cosmetic according to claim 6, characterized in that: The following steps are involved: T1: Mix EDTA-2Na, deionized water and glycerol evenly and stir for 6-10 minutes until they are completely dissolved to obtain product 1; T2: heat isopropyl myristate, cetearyl alcohol, and polyglyceryl-2 distearate to 80°C and mix well to obtain product 2; T3: Mix product 1 and product 2 evenly, cool to 40° C., add the anti-aging composition and phenoxyethanol, stir evenly, and discharge to obtain the cosmetics.

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