An anti-aging radiation device for cell engineering

Abstract

The present invention discloses an anti-aging radiation device for cell engineering, which relates to the field of radiation experiment technology. It comprises a shell, a discharge ring is sealingly and slidably connected to the shell, the shell is provided with discharge holes which are distributed in an annular manner and are all sealed with the discharge ring, the shell is fixed with a distribution plate located above the discharge holes which are distributed in an annular manner, the distribution plate is provided with distribution holes which are distributed in an annular manner, the lower side of the distribution plate is rotatably connected with a shielding ring which is sealed with the distribution holes which are distributed in an annular manner, the shielding ring is provided with a light-transmitting sheet which is distributed in an annular manner, and the shell is provided with feeding holes which are distributed in an annular manner and are respectively located below the adjacent feeding holes. The present invention opens the feeding holes at the position of a single culture dish when sampling, without opening the entire culture box, thereby reducing the area where the environment inside the culture box contacts the external environment during sampling, reducing the influence of the sampling process on the environment inside the culture box, and thus improving the accuracy of the experimental results.

Description

Technical Field

[0001] The invention relates to the technical field of radiation experiments, and in particular to an anti-aging radiation device for cell engineering. Background Art

[0002] Malignant tumors are characterized by their hidden onset and difficulty in cure, which seriously threaten the health of the people. There are three existing methods for treating malignant tumors: surgical resection, radiotherapy and chemotherapy. Among them, radiotherapy uses the characteristics of some tumor cells being sensitive to radiation, and uses radiation to induce DNA damage in such cancer cells and cause cell apoptosis. One of the methods of radiotherapy is to implant radioactive particles into the tumor for treatment. However, since the same type of cancer cells has different radiosensitivities in different patients, it is necessary to understand the radiosensitivity of the cancer cells in the patient before implanting radioactive particles, that is, to understand the ability of cancer cells to resist aging under radiation conditions, and adjust the number and distribution of radioactive particles implanted in the patient's body according to radioactive experimental data. In order to understand the radiosensitivity of cancer cells, the cancer cells in the patient's body are usually sampled, and then an incubator is used to simulate the patient's internal environment and to reproduce and culture the cancer cells. After the number of cancer cells reaches a certain number, the cancer cells are exposed to radiation conditions, and the cancer cells are sampled regularly to observe their survival. However, when sampling the cancer cells in the culture dish, the incubator needs to be fully opened, resulting in the environment in the incubator being completely in contact with the external environment, destroying the environment in the incubator and affecting the accuracy of the experimental results. Summary of the invention

[0003] The present invention provides an anti-aging radiation device for cell engineering, so as to overcome the disadvantage that during the existing radioactive experiment on cells, the cell culture environment will be destroyed when sampling in the middle, thus affecting the accuracy of the experimental results.

[0004] The technical solution is: an anti-aging radiation device for cell engineering, including a shell, a discharge ring is sealingly and slidably connected to the shell, the shell is provided with discharge holes which are distributed in an annular manner and are all sealed with the discharge ring, the shell is fixedly connected with a distribution plate located above the discharge holes distributed in an annular manner, the distribution plate is provided with distribution holes distributed in an annular manner, the lower side of the distribution plate is rotatably connected with a shielding ring which is sealed with the distribution holes distributed in an annular manner, the shielding ring is provided with a light-transmitting sheet distributed in an annular manner, the shell is provided with feeding holes which are distributed in an annular manner and are respectively located below the adjacent discharge holes, and the shell is fixed with a distribution plate which is distributed in an annular manner and is respectively connected with the adjacent feeding holes. A connecting shell, wherein the connecting shell is rotatably connected to a placing piece rotatably connected to the shell, a supporting piece is sealed, slidably and rotatably connected inside the placing piece, a guide groove is provided on the supporting piece, a hemisphere slidably connected to an adjacent guide groove is provided on the placing piece, a limiting cylinder is fixedly connected to a position close to the placing piece in the shell, a sliding piece rotatably connected to an adjacent supporting piece and frictionally fitted is limited in the limiting cylinder, a power assembly providing power for the annularly distributed placing pieces is provided in the shell, a shielding assembly which is annularly distributed and reduces the contact area between the incubator and the external environment, and a supporting assembly which is annularly distributed and improves the stability of the culture dish are provided in the shell.

[0005] Furthermore, the number of the annularly distributed distribution holes is an integer multiple of the number of the annularly distributed placement pieces, and the distribution holes are not within the projection of the placement pieces on the surface of the distribution plate, so as to improve the uniformity of radiation received by cells in the culture dish.

[0006] Furthermore, the power assembly includes a motor, which is installed in the shell, and the output shaft of the motor is fixedly connected to an active gear ring, and the shell is rotatably connected to passive gear rings distributed in an annular manner and respectively rotatably connected to adjacent placing parts, the passive gear rings are meshed with the active gear rings, and the passive gear rings are slidably connected to a three-pronged limit piece, and the placing part is provided with a groove that cooperates with the adjacent three-pronged limit piece, the three-pronged limit piece is rotatably connected to a limiting ring, and the limiting ring is fixedly connected to a control frame slidably connected to the shell, and the control frame is frictionally matched with the shell.

[0007] Furthermore, the friction force between the support member and the adjacent sliding member has a component force on the upper hemisphere of the placement member along the tangent direction of the guide groove that is greater than the component force of the gravity of the support member and the parts thereon along the tangent direction of the guide groove, which is used to control the movement of the support member.

[0008] Furthermore, the shielding assembly includes a shielding film, the shielding film is fixed to a side of the adjacent placing member away from the adjacent passive gear ring, the shielding film is fixed to an elastic ring on a side close to the central axis of the adjacent placing member, the elastic ring is fixed to a ring-shaped draw rope, the sliding member is rotatably connected to a connecting ring, the draw rope passes through the adjacent placing member and is fixed to the adjacent connecting ring.

[0009] Furthermore, the shielding film is made of elastic and light-proof material, and the elastic coefficient of the shielding film is smaller than the elastic coefficient of the elastic ring, so that the shielding film does not shield the adjacent placement piece when no force is applied.

[0010] Furthermore, the supporting assembly includes a three-pronged extrusion piece, which is slidably connected to the side of the adjacent placing piece away from the adjacent passive gear ring, and the three-pronged extrusion piece is rotatably connected to a driving ring fixed to the adjacent control frame, and the placing piece is positionally limited and slidably connected to an annularly distributed extrusion spring piece close to the adjacent three-pronged extrusion piece, and the extrusion spring piece is extruded and matched with the adjacent three-pronged extrusion piece.

[0011] Furthermore, it also includes a bulk material piece, which is rotatably connected to a position inside the shell close to the material distribution plate, the bulk material piece is in contact with the material distribution plate, and the material distribution plate cooperates with the shell to form a distribution cavity that is connected to the annularly distributed material distribution holes, and the position of the bulk material piece away from the central axis of the shell is provided with an inclined surface and a limit groove, and the side of the above-mentioned limit groove away from the central axis of the shell is located on the circle formed by the side of the annularly distributed material distribution holes away from the central axis of the shell.

[0012] Furthermore, the shielding ring is fixedly connected to and connected with a ring-shaped material discharge shell, the ring-shaped material discharge shell and the ring-shaped light-transmitting sheet are staggered, the ring-shaped material discharge shell part is fixedly connected with a connecting piece on one side of the ring-shaped material discharge shell close to the central axis of the shell body, the connecting piece is provided with a material storage groove, and the projection of the position of the material discharge shell close to the central axis of the connecting piece in the horizontal plane is located in the material storage groove.

[0013] Furthermore, the bulk material parts and the distribution plate are sealed, slidably and rotatably connected with a push rod, the push rod is located on one side of the shell and is rotatably connected to a transmission frame, a torsion spring is fixedly connected between the transmission frame and the connecting piece, the output shaft of the motor is fixedly connected to a friction wheel, the friction wheel is frictionally matched with the transmission frame, an extrusion column is slidably connected in the distribution plate, and a spring is fixedly connected between the two, the shielding ring is provided with annularly distributed semicircular grooves, the semicircular grooves are extrusion-matched with the extrusion column, the number of the semicircular grooves is three times the number of the annularly distributed light-transmitting sheets, and the light-transmitting sheets and the unloading shell correspond to adjacent semicircular grooves respectively.

[0014] The advantages and positive effects of the present invention compared with the prior art are as follows: the present invention opens the material collection hole at the position of a single culture dish when sampling, without opening the entire incubator, thereby reducing the contact area between the environment inside the incubator and the external environment during sampling, reducing the impact of the sampling process on the environment inside the incubator, and thereby improving the accuracy of the experimental results.

[0015] The culture dish in the incubator is clamped and fixed by the circumferentially distributed extrusion springs, which improves the stability of the culture dish and reduces the probability of the external environment affecting the cells in the culture dish.

[0016] The radioactive particles are dispersed to the corresponding positions through bulk materials, which improves work efficiency, reduces the length of time doctors are exposed to radiation, and reduces the damage caused to the doctors' bodies by radioactive particles.

[0017] The shielding ring quickly releases the blocking of the material distribution hole through the torsion spring force storage, so that all the culture dishes in the incubator receive radiation at the same time, thereby improving the accuracy of the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0019] Figure 2 It is a three-dimensional structural cross-sectional view of the housing, the discharge ring and the material distribution plate of the present invention;

[0020] Figure 3 It is a three-dimensional structural schematic diagram of the housing, the connecting shell and the placement piece of the present invention;

[0021] Figure 4 It is a schematic diagram of the three-dimensional structure of the housing, the placement member and the support member of the present invention;

[0022] Figure 5 It is a three-dimensional structural coordination diagram of the placement member and the support member of the present invention;

[0023] Figure 6 It is a three-dimensional structural schematic diagram of the support member, the limiting cylinder and the sliding member of the present invention;

[0024] Figure 7 It is a three-dimensional structural schematic diagram of the three-pronged limiting member, limiting ring and control frame of the present invention;

[0025] Figure 8 It is a schematic diagram of the three-dimensional structure of the shielding film, the elastic ring and the drawstring of the present invention;

[0026] Fig. 9 The present invention is attached Figure 4 The enlarged view of point A in the middle;

[0027] Fig.10 It is a three-dimensional structural schematic diagram of the material distribution plate, bulk material parts and material discharge shell of the present invention;

[0028] Fig.11 It is a schematic diagram of the three-dimensional structure of the material distribution plate, the shielding ring and the extrusion column of the present invention;

[0029] Fig.12 It is a schematic diagram of the three-dimensional structure of the blanking shell, the connecting piece and the push rod of the present invention;

[0030] Fig.13 It is a three-dimensional structural schematic diagram of the push rod, transmission frame and friction wheel of the present invention.

[0031] In the above drawings: 1-shell, 2-discharging ring, 201-discharging hole, 3-distributing plate, 301-distributing hole, 4-shielding ring, 401-light-transmitting sheet, 5-connecting shell, 501-feeding hole, 6-placing member, 7-support member, 701-guide groove, 8-limiting cylinder, 9-sliding member, 10-motor, 11-active gear ring, 12-passive gear ring, 13-trident limiting member, 14- Limiting ring, 15-control frame, 16-shielding film, 17-elastic ring, 18-pull rope, 19-connecting ring, 20-three-pronged extrusion piece, 21-driving ring, 22-extrusion spring piece, 23-bulk material piece, 231-distribution cavity, 24-discharging shell, 25-connecting piece, 251-storage trough, 26-push rod, 27-transmission frame, 28-friction wheel, 29-extrusion column, 291-semicircular groove. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0033] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] When conducting radioactive experiments on cancer cells, samples need to be taken at intervals. Since the incubator needs to be opened when taking samples, the environment inside the incubator comes into contact with the external environment, which destroys the environment inside the incubator and affects the accuracy of the experimental results.

[0035] Example 1: An anti-aging radiation device for cell engineering, please refer to Figure 1-Figure 6, including a shell 1, in which a cell culture system is arranged to simulate the temperature and humidity in the patient's body and the content of various substances in the gas, the inner wall of the shell 1 is provided with a radiation-isolating coating, the upper and lower parts of the outer side of the shell 1 are sealed and slidably connected with a discharge ring 2, the discharge ring 2 is a circular ring with an anti-slip groove, and the upper and middle parts of the shell 1 are provided with six discharge holes 201 distributed in an annular manner. The doctor puts the culture dish into the shell 1 through the discharge holes 201, and the discharge holes 201 are sealed and matched with the discharge ring 2 to isolate the environment inside the shell 1 from the external environment, and a distribution plate 3 is fixedly connected to the upper part of the shell 1, and the distribution plate 3 is located above the six discharge holes 201, and a plurality of distribution holes 301 distributed in an annular manner are provided on the distribution plate 3, and the distribution holes 301 are used to place radioactive particles, and a single distribution hole 301 is used to place radioactive particles. The material hole 301 can only accommodate one radioactive particle. The number of the annular distribution holes 301 is an integer multiple of the number of the annular distribution placement pieces 6 (here it is doubled, that is, the number of the distribution holes 301 is twelve), and the distribution holes 301 are not within the projection of the placement piece 6 on the surface of the distribution plate 3. The projection of the line connecting two adjacent radioactive particles on the adjacent culture dish below passes through the central axis of the culture dish (the distance between each point on the culture dish and the adjacent radioactive particle is inversely proportional to the radiation intensity received by each point on the culture dish). It is used to make the cells at various locations in the culture dish on the placement piece 6 receive the same amount of radiation when it rotates. The lower side of the distribution plate 3 is rotatably connected to a shielding ring 4, which is made of opaque material. The distribution holes 301 are sealed and matched with the shielding ring 4. The shielding ring 4 Twelve light-transmitting sheets 401 distributed in an annular pattern are arranged on the top, and the light-transmitting sheets 401 are made of glass. Six feeding holes 501 distributed in an annular pattern are arranged in the middle of the peripheral side of the shell 1, and the discharge hole 201 is located above the adjacent feeding hole 501. A connecting shell 5 distributed in an annular pattern is fixedly connected in the shell 1, and the connecting shell 5 is connected with the adjacent feeding hole 501. The connecting shell 5 is rotatably connected with a placing member 6, and the placing member 6 is rotatably connected with the shell 1. The lower seal in the placing member 6 slides and is rotatably connected with a supporting member 7, and a guide groove 701 is arranged on the supporting member 7. A hemisphere is arranged on the peripheral side of the placing member 6, and the hemisphere on the placing member 6 is slidably connected with the adjacent guiding groove 701, which is used to guide the supporting member 7 to move up and down. A limiting cylinder 8 is fixedly connected to the lower side of the shell 1 near the position of the placing member 6, and the limiting cylinder The upper and lower limit sliding connections 8 are connected with a sliding member 9, which is composed of a cylinder and three connecting blocks fixed at the bottom thereof. The upper part of the cylinder of the sliding member 9 is rotatably connected to the lower side of the middle part of the adjacent support member 7, and the two are frictionally matched. The friction force between the support member 7 and the adjacent sliding member 9 is greater than the component of the gravity of the support member 7 and its parts on the adjacent placement member 6 in the tangential direction of the upper hemisphere of the guide groove 701, which is used to make the support member 7 move up first and then stop. A power component that provides power for the six placement members 6 is arranged in the shell 1, and a shielding component that is annularly distributed and used to reduce the contact area between the incubator and the external environment and a support component that is annularly distributed and used to improve the stability during the culture dish experiment are arranged in the shell 1.

[0036] Please refer to Figure 2-Figure 7 The power assembly includes a motor 10, which is installed in the middle of the lower side of the shell 1. The output shaft of the motor 10 is fixedly connected with an active gear ring 11. A ring-shaped passive gear ring 12 is rotatably connected in the shell 1. The passive gear ring 12 is rotatably connected to the lower side of the adjacent placement member 6. The passive gear ring 12 meshes with the active gear ring 11. The passive gear ring 12 is slidably connected with a three-pronged limit member 13 up and down. The lower side of the placement member 6 is provided with three ring-shaped grooves. The three grooves on the lower side of the placement member 6 are together limited by the adjacent three-pronged limit member 13 to enable the passive gear ring 12 to drive the adjacent placement member 6 to rotate. The three-pronged limit member 13 is rotatably connected with a limit ring 14. The limit ring 14 is fixedly connected with a control frame 15. The control frame 15 is slidably connected to the shell 1. The control frame 15 and the shell 1 are frictionally matched, and the friction between the two is greater than the gravity of the control frame 15 and all parts thereon, which is used to maintain the position of the control frame 15 relative to the shell 1.

[0037] Please refer to Figure 2 , Figure 3 , Figure 7 and Figure 8 The shielding component includes a shielding film 16 (made of existing elastic opaque material, not specifically shown in the attached drawings), the shielding film 16 is fixedly connected to the upper side of the adjacent placement piece 6, an elastic ring 17 is fixedly connected to the middle of the shielding film 16, and the elastic coefficient of the shielding film 16 is smaller than the elastic coefficient of the elastic ring 17. Initially, the shielding film 16 is stretched by the adjacent elastic ring 17. When the shielding film 16 is not subjected to force, the inner diameter of the shielding film 16 is larger than the inner diameter of the upper part of the placement piece 6. The elastic ring 17 is fixedly connected to three ring-shaped pull ropes 18 (the number of the pull ropes 18 can be changed according to actual conditions, and the number of pull ropes 18 shown in the attached drawings is three). When the elastic ring 17 is pulled to the limit state by the three adjacent pull ropes 18, the shielding film 16 does not block the adjacent placement piece 6. The three connecting blocks at the lower part of the sliding piece 9 are rotatably connected to a connecting ring 19, and the pull rope 18 passes through the adjacent placement piece 6 and is fixedly connected to the lower side of the adjacent connecting ring 19.

[0038] Please refer to Figure 3-Figure 6 and Fig. 9 The supporting assembly includes a three-pronged extrusion member 20. There is an inclined surface at the upper part of the three-pronged extrusion member 20 near its central axis, and the lower part of the inclined surface is a vertical surface. The three-pronged extrusion member 20 is slidably connected to the upper part of the adjacent placement member 6. The three-pronged extrusion member 20 is rotatably connected to a driving ring 21 fixed to the adjacent control frame 15. The upper limit sliding connection in the placement member 6 is three annularly distributed extrusion springs 22. The extrusion springs 22 are made of elastic material and are used to fix the culture dish. The extrusion springs 22 are extruded and matched with the adjacent three-pronged extrusion member 20.

[0039] When studying the anti-aging properties of cancer cells under radiation conditions, the doctor samples cancer cells from the patient's body, divides the sampling results into six parts and puts them into culture dishes respectively, then the doctor lifts the control frame 15 upward (later explained with a group of parts on the right side of the shell 1), the control frame 15 drives the limiting ring 14 and the driving ring 21 to move upward (the control frame 15 then stops under the action of the friction between it and the shell 1), the limiting ring 14 drives the three-pronged limiting member 13 to move upward, the three-pronged limiting member 13 is inserted into the grooves of the three placement members 6 and limits the placement members 6, and at the same time the driving ring 21 drives the three-pronged extrusion member 20 to move upward, the inclined surface on the three-pronged extrusion member 20 squeezes the three extrusion springs 22 to move in the direction close to the central axis of the placement member 6, until the vertical surface on the three-pronged extrusion member 20 contacts the three extrusion springs 22 (at this time, the diameter of the circle formed by the three extrusion springs 22 is smaller than the outer diameter of the culture dish), and the control frame 15 stops moving.

[0040] After the control frame 15 stops moving, the doctor starts the motor 10, and the output shaft of the motor 10 rotates counterclockwise (the rotation perspective in this article is from top to bottom) and drives the active gear ring 11 to rotate. The active gear ring 11 drives the placement member 6 to rotate through the passive gear ring 12 and the three-pronged limit member 13. The placement member 6 slides along the guide groove 701 and squeezes the support member 7 upward (at this time, due to the friction between the support member 7 and the sliding member 9, the support member 7 and the sliding member 9 simply move up and down together), until the upper side of the support member 7 contacts the placement member 6, the support member 7 stops moving up, and then the placement member 6 drives the support member 7 to rotate through the guide groove 701, causing friction between the support member 7 and the sliding member 9.

[0041] During the upward movement of the support member 7, the support member 7 drives the connecting ring 19 to move upward through the sliding member 9. The connecting ring 19 pulls the three pull ropes 18. The three pull ropes 18 jointly pull the elastic ring 17, thereby increasing the circumference of the elastic ring 17. The inner diameter of the shielding film 16 also increases under the action of its own elasticity, and the shielding film 16 releases the shielding of the upper side of the placement member 6.

[0042] After the support member 7 stops moving upward, the doctor moves the discharge ring 2 upward to release the blocking of the six discharge holes 201 by the discharge ring 2. Then the doctor places the culture dish on the upper side of the support member 7 through the six discharge holes 201 in turn. When placing the culture dish, the culture dish first contacts with the three squeezing springs 22 and squeezes its deformation. The culture dish is fixed by the three squeezing springs 22, thereby improving the stability of the culture dish. After the doctor places all six culture dishes in the shell 1, the doctor moves the discharge ring 2 downward and resets it to make the discharge ring 2 block the discharge hole 201 again. Then the doctor starts the cell culture system in the shell 1 and simulates the environment in the patient's body (temperature, humidity and the content of various substances in the gas), and starts to reproduce and culture the cells. After the cells are cultured to a certain extent, the doctor places radioactive particles in the distribution hole 301, and the radioactive particles radiate the cancer cells in the culture dish through the adjacent light-transmitting sheet 401, and then starts timing.

[0043] After the doctor has placed the radioactive particles for a period of time, the doctor pulls the control frame 15 on the right side downward, and the control frame 15 drives the three-pronged limiting member 13 to move down and reset through the limiting ring 14, so that the three-pronged limiting member 13 loses contact with the groove on the placement member 6 and releases the limiting of the placement member 6 (at this time, the placement member 6 stops rotating). At the same time, the control frame 15 drives the three-pronged extrusion member 20 to move down and reset through the driving ring 21, and releases the extrusion of the three-pronged extrusion member 20 on the extrusion spring 22, so that the extrusion spring 22 moves in the direction away from the central axis of the placement member 6 under its own reset action (at this time, the culture dish loses the extrusion of the extrusion spring 22).

[0044] After the doctor moves the control frame 15 downward and resets it, the placement member 6 is squeezed in the opposite direction by the guide groove 701 under the action of the gravity of the culture dish and the support member 7, so that the support member 7 drives the culture dish downward and resets, and at the same time, the sliding member 9 drives the connecting ring 19 downward. At this time, the shielding film 16 is reset under the elastic action of the elastic ring 17 and the pull rope 18 is reset, so that the shielding film 16 again blocks the upper side of the placement member 6. At this time, the doctor takes out the culture dish on the upper side of the support member 7 through the material taking hole 501, and then repeats the above steps to take out six culture dishes at intervals. By observing the survival of cancer cells in the culture dishes, experimental data is obtained. The experiment is now completed, and the doctor turns off the cell culture system and the motor 10. Then the doctor takes out and recovers the radioactive particles in the dispensing hole 301.

[0045] When doctors use radioactive particles to conduct radiation experiments on cancer cells, they are usually required to distribute the radioactive particles evenly so that the radiation intensity of the radioactive particles on cancer cells in various locations in the culture dish is consistent. During this process, the doctor is in direct contact with the radioactive particles, which may cause harm to the doctor.

[0046] Example 2: Based on Example 1, please refer to Figure 2 , Fig.10 and Fig.11 , and also includes a bulk material piece 23, which is rotatably connected to the upper part of the shell 1, and the bulk material piece 23 contacts the upper side of the distribution plate 3. The upper side of the distribution plate 3 cooperates with the shell 1 to form a distribution cavity 231 connected to all the distribution holes 301. The middle part of the bulk material piece 23 is provided with two front and rear guide holes for guiding external radioactive particles to the front and rear sides of the distribution cavity 231. The position of the bulk material piece 23 away from the central axis of the shell 1 is provided with an inclined surface and a limiting groove, and the end of the above-mentioned limiting groove is located on the circle formed by the side of the annular distribution distribution holes 301 away from the central axis of the shell 1, which is used to put the radioactive particles in the limiting groove into the adjacent distribution holes 301.

[0047] Please refer to Fig.10 , Fig.12 and Fig.13 Twelve annularly distributed material shells 24 are fixedly connected and communicated with the shielding ring 4. The annularly distributed material shells 24 are staggered with the annularly distributed light-transmitting sheets 401. A connecting piece 25 is commonly fixedly connected to the lower sides of three material shells 24. A material storage groove 251 is provided on the connecting piece 25. The projection of the position of the material shell 24 close to the central axis of the connecting piece 25 in the horizontal plane is located in the material storage groove 251. The material storage groove 251 is used to collect radioactive particles.

[0048] Please refer to Figure 11-13 The middle part of the bulk material part 23 and the middle part of the material distribution plate 3 are sealed, slidably and rotatably connected with a push rod 26. The lower part of the push rod 26 is rotatably connected with a transmission frame 27. A torsion spring is fixedly connected between the transmission frame 27 and the lower side of the connecting member 25. The output shaft of the motor 10 is fixedly connected with a friction wheel 28. The friction wheel 28 and the transmission frame 27 are frictionally matched. Initially, the friction wheel 28 and the transmission frame 27 are not in contact. An extrusion column 29 is slidably connected to the left and right inside the material distribution plate 3. The right side of the extrusion column 29 is connected to the A spring is fixed between the material distribution plates 3, and an annularly distributed semicircular grooves 291 are provided on the circumferential side of the shielding ring 4. The semicircular grooves 291 are squeezed and matched with the extrusion columns 29 to limit the shielding ring 4. Initially, one of the semicircular grooves 291 corresponding to the adjacent light-transmitting sheet 401 contacts the extrusion column 29. The number of the semicircular grooves 291 is three times the number of the annularly distributed light-transmitting sheets 401, and the light-transmitting sheets 401 and the unloading shell 24 correspond to the adjacent semicircular grooves 291, respectively.

[0049] When conducting a radioactive experiment, the steps of Example 1 are repeated, the motor 10 is started, and the culture dish is placed in the shell 1. Then the doctor presses the push rod 26 downward, and the push rod 26 pushes the transmission frame 27 downward, stretching the torsion spring adjacent to the transmission frame 27. At the same time, the transmission frame 27 contacts with the friction wheel 28 and frictionally transmits the transmission. As the output shaft of the motor 10 drives the transmission frame 27 to rotate through the friction wheel 28, the torsion spring adjacent to the transmission frame 27 is twisted. The transmission frame 27 drives the shielding ring 4 to rotate counterclockwise through the adjacent torsion springs and the three connected discharge shells 24. Until the torsion force of the torsion spring overcomes the elastic force of the adjacent spring of the extrusion column 29, the adjacent torsion spring of the transmission frame 27 drives the shielding ring 4 rotates, the shielding ring 4 squeezes the extrusion column 29 to the right through the semicircular groove 291, compresses the spring adjacent to the extrusion column 29, and releases the extrusion column 29 from limiting the shielding ring 4. As the shielding ring 4 rotates, the shielding ring 4 drives all the discharge shells 24 to rotate, until the extrusion column 29 gradually corresponds to the next semicircular groove 291 and enters the above-mentioned semicircular groove 291 under the action of its adjacent spring, limiting the shielding ring 4. At this time, the discharge shell 24 corresponds to the adjacent material distribution hole 301. As the adjacent torsion spring of the transmission frame 27 continues to accumulate force and overcomes the elastic force of the adjacent spring of the extrusion column 29 again, the above-mentioned steps of rotating the shielding ring 4 are repeated, so that the shielding ring 4 blocks all the material distribution holes 301.

[0050] After the shielding ring 4 blocks all the distributing holes 301, the doctor stops pressing the push rod 26, and the push rod 26 and the transmission frame 27 move up and reset under the reset action of the adjacent torsion springs. Then the doctor puts twelve radioactive particles into the bulk material piece 23, and then the radioactive particles enter the front and rear sides of the distribution cavity 231 through the two guide holes in the bulk material piece 23. At this time, the doctor rotates the bulk material piece 23 clockwise, and the bulk material piece 23 contacts the radioactive particles in the distribution cavity 231 and drives it to rotate circumferentially. While the radioactive particles rotate circumferentially, the radioactive particles move along the bulk material piece 23 in a direction away from the central axis of the shell 1 (that is, the actual movement trajectory of the radioactive particles is spiral), and the radioactive particles gradually enter the two sides of the bulk material piece 23. The doctor then stops rotating the bulk material piece 23 until the radioactive particles respectively enter the adjacent material distribution holes 301 (at this time, the sound of radioactive particles rolling along the surface of the distribution plate 3 is no longer generated when the bulk material piece 23 rotates). By rotating the bulk material piece 23, the doctor's direct contact time with the radioactive particles is reduced, thereby reducing the damage caused to the doctor by the radioactive particles.

[0051] After the doctor stops rotating the bulk material part 23, the above-mentioned step of pressing the push rod 26 is repeated, and the shielding ring 4 rotates again. Under the action of the stored force of the adjacent torsion spring of the connecting member 25, the shielding ring 4 rotates rapidly (reducing the time difference of the radiation received by the cancer cells at various locations on the culture dish and improving the uniformity of the radiation received by the cancer cells at various locations in the culture dish), and releases the blockage of the material distribution hole 301, and at the same time makes the light-transmitting sheet 401 correspond to the adjacent material distribution hole 301. At this time, the radioactive particles in the material distribution hole 301 pass through the light-transmitting sheet 401 to radiate the cells in the culture dish below it, and then repeat the steps of Example 1, take out the culture dishes at intervals, and count the experimental data. The doctor then repeats the above-mentioned step of pressing the push rod 26, and the shielding ring 4 rotates again. At this time, the discharge shell 24 corresponds to the adjacent distribution hole 301, and the radioactive particles in the distribution hole 301 enter the adjacent discharge shell 24, slide along the discharge shell 24 and finally fall into the storage trough 251. At this time, the collection of the radioactive particles is completed. The doctor then moves the discharge ring 2 upward and takes out the radioactive particles in the storage trough 251 through the discharge hole 201. By collecting the radioactive particles in the storage trough 251, it is convenient for the doctor to recover them. At the same time, the time when the doctor directly contacts the radioactive particles is reduced, thereby reducing the damage caused to the doctor by the radioactive particles.

[0052] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention are included in the protection scope of the present invention.

Claims

1. An anti-aging radiation device for cell engineering, characterized by: The invention comprises a shell, a discharge ring is sealingly and slidably connected to the shell, the shell is provided with discharge holes which are distributed in an annular manner and are all sealed with the discharge ring, the shell is fixedly connected with a distribution plate which is located above the discharge holes which are distributed in an annular manner, the distribution plate is provided with distribution holes which are distributed in an annular manner, a shielding ring which is sealed with the distribution holes which are distributed in an annular manner and are matched, the shielding ring is provided with a light-transmitting sheet which is distributed in an annular manner, the shell is provided with material taking holes which are distributed in an annular manner and are respectively located below the adjacent discharge holes, a connecting shell which is distributed in an annular manner and is respectively connected with the adjacent material taking holes is fixedly connected in the shell, and the connecting shell is rotatably connected with the lower side of the distribution plate. A placing piece is rotatably connected with the shell, a supporting piece is sealed and slidably and rotatably connected inside the placing piece, a guide groove is provided on the supporting piece, a hemisphere slidably connected with an adjacent guide groove is provided on the placing piece, a limiting cylinder is fixedly connected to a position close to the placing piece in the shell, a sliding piece rotatably connected with an adjacent supporting piece and frictionally engaged is limitedly slidably connected in the limiting cylinder, a power assembly providing power for the annularly distributed placing pieces is provided in the shell, a shielding assembly which is annularly distributed and reduces the contact area between the incubator and the external environment, and a supporting assembly which is annularly distributed and improves the stability of the culture dish are provided in the shell; The power assembly includes a motor, which is installed in the housing, the output shaft of the motor is fixedly connected to an active gear ring, the housing is rotatably connected to passive gear rings distributed in an annular manner and respectively rotatably connected to adjacent placement members, the passive gear rings mesh with the active gear rings, the passive gear rings are slidably connected to a three-pronged limiter, the placement member is provided with a groove that is limitedly matched with the adjacent three-pronged limiter, the three-pronged limiter is rotatably connected to a limit ring, the limit ring is fixedly connected to a control frame that is slidably connected to the housing, and the control frame is frictionally matched with the housing; The shielding assembly includes a shielding film, the shielding film is fixed to a side of the adjacent placement member away from the adjacent passive gear ring, the shielding film is fixed to an elastic ring on a side close to the central axis of the adjacent placement member, the elastic ring is fixed to a ring-shaped drawstring, the sliding member is rotatably connected to a connecting ring, the drawstring passes through the adjacent placement member and is fixed to the adjacent connecting ring; The supporting assembly includes a three-pronged extrusion piece, which is slidably connected to the side of the adjacent placement piece away from the adjacent passive gear ring. The three-pronged extrusion piece is rotatably connected to a driving ring fixed to the adjacent control frame. The placement piece is positionally limited and slidably connected to an annularly distributed extrusion spring sheet close to the adjacent three-pronged extrusion piece, and the extrusion spring sheet is extruded and matched with the adjacent three-pronged extrusion piece.

2. The anti-aging radiation device for cell engineering according to claim 1, characterized in that: The number of the annularly distributed material distribution holes is an integer multiple of the number of the annularly distributed placement pieces, and the material distribution holes are not within the projection of the placement pieces on the surface of the material distribution plate, so as to improve the uniformity of radiation received by cells in the culture dish.

3. The anti-aging radiation device for cell engineering according to claim 1, characterized in that: The friction force between the support and the adjacent sliding member has a component force on the upper hemisphere of the placement member along the tangent direction of the guide groove that is greater than the component force of the gravity of the support and the parts thereon along the tangent direction of the guide groove on the upper hemisphere of the placement member, which is used to control the movement of the support.

4. The anti-aging radiation device for cell engineering according to claim 1, characterized in that: The shielding film is made of an elastic, light-proof material, and the elastic coefficient of the shielding film is smaller than the elastic coefficient of the elastic ring, so that the shielding film does not shield the adjacent placement piece when no force is applied.

5. The anti-aging radiation device for cell engineering according to claim 1, characterized in that: It also includes a bulk material piece, which is rotatably connected to a position inside the shell close to the material distribution plate, the bulk material piece is in contact with the material distribution plate, and the material distribution plate cooperates with the shell to form a distribution cavity that is connected to the annularly distributed material distribution holes. The position of the bulk material piece away from the central axis of the shell is provided with an inclined surface and a limit groove, and the side of the limit groove away from the central axis of the shell is located on a circle formed by the side of the annularly distributed material distribution holes away from the central axis of the shell.

6. The anti-aging radiation device for cell engineering according to claim 5, characterized in that: A ring-shaped material discharge shell is fixedly connected to and communicated with the shielding ring. The ring-shaped material discharge shell is staggered with the ring-shaped light-transmitting sheet. A connecting piece is fixedly connected to one side of the ring-shaped material discharge shell close to the central axis of the shell body. A material storage groove is arranged on the connecting piece. The projection of the position of the material discharge shell close to the central axis of the connecting piece in the horizontal plane is located in the material storage groove.

7. The anti-aging radiation device for cell engineering according to claim 6, characterized in that: The bulk material parts and the distribution plate are sealed, slidably and rotatably connected with a push rod, and the push rod is located on one side of the shell and is rotatably connected to a transmission frame, a torsion spring is fixedly connected between the transmission frame and the connecting piece, and the output shaft of the motor is fixedly connected to a friction wheel, and the friction wheel is frictionally matched with the transmission frame, an extrusion column is slidably connected in the distribution plate, and a spring is fixedly connected between the two, and the shielding ring is provided with annularly distributed semicircular grooves, and the semicircular grooves are extrusion-matched with the extrusion column, and the number of the semicircular grooves is three times the number of the annularly distributed light-transmitting sheets, and the light-transmitting sheets and the unloading shell correspond to adjacent semicircular grooves respectively.

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